Publications by authors named "Qianqian Huang"

100 Publications

UHPLC/MS-Based Serum Metabolomics Reveals the Mechanism of Radiation-Induced Thrombocytopenia in Mice.

Int J Mol Sci 2022 Jul 20;23(14). Epub 2022 Jul 20.

School of Pharmacy, Southwest Medical University, Luzhou 646000, China.

Radiation-induced thrombocytopenia is a common and life-threatening side effect of ionizing radiation (IR) therapy. However, the underlying pathological mechanisms remain unclear. In the present study, irradiation was demonstrated to significantly reduce platelet levels, inhibit megakaryocyte differentiation, and promote the apoptosis of bone marrow (BM) cells. A metabolomics approach and a UHPLC-QTOF MS system were subsequently employed for the comprehensive analysis of serum metabolic profiles of normal and irradiated mice. A total of 66 metabolites were significantly altered, of which 56 were up-regulated and 10 were down-regulated in irradiated mice compared to normal mice on day 11 after irradiation. Pathway analysis revealed that disorders in glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, sphingolipid metabolism, inositol phosphate metabolism, and tryptophan metabolism were involved in radiation-induced thrombocytopenia. In addition, three important differential metabolites, namely L-tryptophan, LysoPC (17:0), and D-sphinganine, which were up-regulated in irradiated mice, significantly induced the apoptosis of K562 cells. L-tryptophan inhibited megakaryocyte differentiation of K562 cells. Finally, serum metabolomics was performed on day 30 (i.e., when the platelet levels in irradiated mice recovered to normal levels). The contents of L-tryptophan, LysoPC (17:0), and D-sphinganine in normal and irradiated mice did not significantly differ on day 30 after irradiation. In conclusion, radiation can cause metabolic disorders, which are highly correlated with the apoptosis of hematopoietic cells and inhibition of megakaryocyte differentiation, ultimately resulting in thrombocytopenia. Further, the metabolites, L-tryptophan, LysoPC (17:0), and D-sphinganine can serve as biomarkers for radiation-induced thrombocytopenia.
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http://dx.doi.org/10.3390/ijms23147978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319504PMC
July 2022

Nanoparticle-Driven Controllable Mitochondrial Regulation through Lysosome-Mitochondria Interactome.

ACS Nano 2022 Jul 25. Epub 2022 Jul 25.

CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Sciences and National Center for Nanoscience and Technology of China, Beijing 100190, China.

Precise subcellular manipulation remains challenging in quantitative biological studies. After target modification and hierarchical assembly, nanoparticles can be functionalized for intracellular investigation. However, it remains unclear whether nanoparticles themselves can progressively manipulate subcellular processes, especially organellar networks. Mitochondria act as the energetic supply, whose fission dynamics are often modulated by molecular reagents. Here, using different-sized gold nanoparticles (AuNPs) as a model, we demonstrated the nanoparticle-driven controllable regulation on mitochondria. Compared with molecular reagents, AuNPs could induce size-dependent mitochondrial fission without detectable cell injury, and this process was reversible along with intracellular AuNPs' clearance. Mechanistically, it was attributed to the AuNPs-induced enhanced organelle interactome between lysosomes and mitochondria. Lysosomal accumulation of AuNPs induced lysosomal swelling and lysosomal motility alterations, promoting mitochondrial fission through the increased "kiss" events during the "kiss-and-run" moving of the lysosome-mitochondria interactome. This study highlights the fundamental understanding to fully explore the intrinsic capability of nanoparticles by engineering their basic properties. Also, it provides practical guidance to investigate the delicate nanolevel regulation on biological processes.
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http://dx.doi.org/10.1021/acsnano.2c04078DOI Listing
July 2022

Asafoetida exerts neuroprotective effect on oxidative stress induced apoptosis through PI3K/Akt/GSK3β/Nrf2/HO-1 pathway.

Chin Med 2022 Jul 6;17(1):83. Epub 2022 Jul 6.

School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.

Background: Alzheimer's Disease (AD) is a serious neurodegenerative disease and there is currently no effective treatment for AD progression. The use of TCM as a potential treatment strategy for AD is an evolving field of investigation. Asafoetida (ASF), an oleo-gum-resin isolated from Ferula assa-foetida root, has been proven to possess antioxidative potential and neuroprotective effects, which is closely associated with the neurological disorders. However, the efficacy and further mechanisms of ASF in AD experimental models are still unclear.

Methods: A cognitive impairment of mouse model induced by scopolamine was established to determine the neuroprotective effects of ASF in vivo, as shown by behavioral tests, biochemical assays, Nissl staining, TUNEL staining, Immunohistochemistry, western blot and qPCR. Furthermore, the PC12 cells stimulated by HO were applied to explore the underlying mechanisms of ASF-mediated efficacy. Then, the UPLCM analysis and integrated network pharmacology approach was utilized to identified the main constitutes of ASF and the potential target of ASF against AD, respectively. And the main identified targets were validated in vitro by western blot, qPCR and immunofluorescence staining.

Results: In vivo, ASF treatment significantly ameliorated cognitive impairment induced by scopolamine, as evidenced by improving learning and memory abilities, and reducing neuronal injury, cholinergic system impairment, oxidative stress and apoptosis in the hippocampus of mice. In vitro, our results validated that ASF can dose-dependently attenuated HO-induced pathological oxidative stress in PC12 cells by inhibiting ROS and MDA production, as well as promoting the activities of SOD, CAT, GSH. We also found that ASF can significantly suppressed the apoptosis rate of PC12 cells increased by HO exposure, which was confirmed by flow cytometry analysis. Moreover, treatment with ASF obviously attenuated HO-induced increase in caspase-3 and Bax expression levels, as well as decrease in Bcl-2 protein expression. KEGG enrichment analysis indicated that the PI3K/Akt/GSK3β/Nrf2 /HO-1pathway may be involved in the regulation of cognitive impairment by ASF. The results of western blot, qPCR and immunofluorescence staining of vitro assay proved it.

Conclusions: Collectively, our work first uncovered the significant neuroprotective effect of ASF in treating AD in vivo. Then, we processed a series of vitro experiments to clarify the biological mechanism action. These data demonstrate that ASF can inhibit oxidative stress induced neuronal apoptosis to foster the prevention of AD both in vivo and in vitro, and it may exert the function of inhibiting AD through PI3K/Akt/GSK3β/Nrf2/HO-1pathway.
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http://dx.doi.org/10.1186/s13020-022-00630-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258148PMC
July 2022

Strategy for colorimetric and reversible recognition of strong acid in solution, solid, and dyed fabric conditions: Substitution of aminophenoxy groups to phthalocyanine.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 28;280:121565. Epub 2022 Jun 28.

Department of Molecular Science and Technology, Ajou University, Suwon 16499, Republic of Korea. Electronic address:

A series of novel peripherally tetra- and octa-substituted copper phthalocyanines (CuPcs) bearing various aminophenoxy groups was designed and synthesized for detecting strong Brønsted acids. Octa-(diethyl-aminophenoxy)-substituted CuPc 5 exhibited excellent HCl detection capability with high sensitivity (limit of detection: 240 ppb), rapid (<2s), and selectivity for strong acids in versatile conditions including solution, solid, and dyed fabric. Furthermore, CuPc 5 noted reusability in recyclable tests with HCl and NH, demonstrating its great potential for practical detection of HCl and ammonia gas leak under various environments. Based on systemic characterizations based on UV-Vis absorption spectra and NMR, we suggest that the proton of HCl associated with the N atom of CuPc 5, and the proton sensing abilities are directly related to the dissociation constants of the amine groups. The steric hindrance of alkyl chains and molar absorption coefficient of the CuPc species in THF solvent, as well as the HO content of the solvent system, also affected the sensing performance. Due to less bulky nature of diethyl-amino groups having higher pK and stronger basicity, CuPc 5 featured effective recognition of strong acids with pK value less than -2.0 (K > 100). To the best of our knowledge, this is the first demonstration of pKa-sensitive colorimetric chemosensor using CuPc backbone, in particular for distinguishing strong Brønsted acids such as HCl.
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http://dx.doi.org/10.1016/j.saa.2022.121565DOI Listing
November 2022

Construction and Biological Evaluation of Multiple Modification Hollow Mesoporous Silicone Doxorubicin Nanodrug Delivery System.

AAPS PharmSciTech 2022 Jun 27;23(6):180. Epub 2022 Jun 27.

College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, Anhui, China.

The combination of functionalized nanoparticles and chemotherapy drugs can effectively target tumor tissue, which can improve efficacy and reduce toxicity. In this article, [email protected] nanoparticles (phosphopeptide-modified polydopamine encapsulates doxorubicin-loaded hollow mesoporous organosilica nanoparticles) were constructed that based on multiple modification hollow mesoporous organosilica nanoparticles (HMONs). The [email protected] nanoparticles retain the biological functions of phosphorylated peptide while exhibiting biological safety that are suitable for effective drug delivery and stimulus responsive release. The degradation behaviors showed that [email protected] has dual-responsive to drug release characteristics of pH and glutathione (GSH). In addition, the prepared [email protected] nanoparticles have good biological safety, and their anti-tumor efficacy was significantly better than doxorubicin (DOX). This provided new research ideas for the construction of targeted nanodrug delivery systems based on mesoporous silicon. Scheme 1 The preparation of [email protected] and the process of drug release under multiple responses. (A) Schematic diagram of the synthesis process of [email protected] (B) The process in which nanoparticles enter the cell and decompose and release DOX in response to pH and GSH.
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http://dx.doi.org/10.1208/s12249-022-02226-8DOI Listing
June 2022

Improving the applicability of the thermo-physiological human simulator by correcting its local set point skin temperatures.

Int J Biometeorol 2022 Aug 25;66(8):1639-1651. Epub 2022 Jun 25.

College of Fashion and Design, Donghua University, West Yan'an Road 1882, Shanghai, 200051, China.

The thermo-physiological human simulator has been used in many regions for estimating thermal behavior of the locals. The applicability of the human simulator to populations from different regions is, however, questioned due to its lack of consideration for the ethnic diversities in thermoregulation. This study checked the potential of improving the applicability of the Newton human simulator, one of the most popular simulators, by correcting its local set point skin temperatures according to the target population (Chinese as an example). First, new set point skin temperatures were obtained by conducting tests with 101 Chinese under a thermal neutral condition. Then, simulator tests using the original and new set point skin temperatures were conducted separately for evaluating thermal responses of the Chinese under non-neutral conditions. The evaluated skin and core temperatures by the simulators were compared with those measured from the real human tests. It demonstrated that the evaluated skin temperatures are positively related with the set point skin temperatures of the simulator. Adjusting set point skin temperatures according to the Chinese improved the prediction performance of the local skin temperatures, with the root-mean-square-deviation being reduced for over 50% of the body segments. The proposed idea of correcting local set point skin temperatures would contribute to evaluating the thermal interaction between human body and its surroundings with a higher accuracy.
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http://dx.doi.org/10.1007/s00484-022-02307-0DOI Listing
August 2022

The Efficacy of Extract in the Treatment of Insomnia: A Systematic Review and Meta-Analysis.

Front Neurol 2022 6;13:730311. Epub 2022 Jun 6.

Department of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.

Aim: This systematic review and meta-analysis was performed to assess the effect of () extract in the treatment of insomnia.

Methods: PubMed, EMBASE, and Cochrane Library databases, as well as references of related articles, were searched. Finally, four articles with five clinical trials including 224 patients were included in the analysis.

Results: The obtained results indicated a greater improvement in total PSQI scores (MD,-4.67; = 0.0002), subjective sleep quality score (MD,-0.91; = 0.003), sleep duration score (MD,-0.77; < 0.00001), and ISI score (MD,-6.30; = 0.009) in the Viola extract group compared with the placebo group. However, the Viola extract group did not significantly differ in sleep latency (MD,-0.85; = 0.08), habitual sleep efficiency (MD,-0.61; = 0.21), sleep disturbances (MD,-0.36; = 0.11), and daytime dysfunction (MD,-0.94; = 0.12) from the placebo group.

Conclusions: extract supplement led to a greater improvement in total PSQI scores, subjective sleep quality score, sleep duration score, and ISI score compared with the placebo group of patients with chronic insomnia.
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http://dx.doi.org/10.3389/fneur.2022.730311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207380PMC
June 2022

The spatio-temporal change in soil P and P-solubilizing bacteria under clover mulching in apple orchards of Loess Plateau.

Chemosphere 2022 Oct 13;304:135334. Epub 2022 Jun 13.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A & F University, Yangling, 712100, Shaanxi, China.

Cover crop is an effective practice for improving soil quality and increase soil nutrients. However, the spatio-temporal change of soil phosphorus (P) components and P-solubilizing microorganisms in the process of grass succession is not evident. Here, we studied the variation of soil P components and P-solubilizing bacteria at 0-60 cm soil layer under clean tillage (CT) and white clover (WC, Trifolium repens L.) grown for 5, 9, and 14 years in an apple test station on the Loess Plateau, China. This study suggested that clover cover could effectively increase the total P, available P (AP), microbial P, organic P (Po), and inorganic P (Al-P, Ca-P, Ca-P and Fe-P) in topsoil (0-20 cm) and AP, Po and inorganic P at 20-40 cm soil layer to improve the soil P bioavailability. The effects of WC living mulch on the soil P forms were more significant with the increase in grass growing years, but this effect was difficult to extend to deep soil. In addition, the WC treatments were beneficial to the growth of P-solubilizing microorganisms in surface soil and improved the alkaline phosphatase activity at 0-40 cm soil layer, mainly including Bacillus, Bradyrhizobium, Nocardioides, Sphingomonas and Streptomyces. This study provided a perspective on the dynamic changes of soil P forms and P-solubilizing microorganisms and under long-term cover crop.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135334DOI Listing
October 2022

Carbon dots derived from polysaccharide as an effective "on-off" fluorescence sensor for chromium (VI) detection.

J Pharm Anal 2022 Feb 1;12(1):104-112. Epub 2021 May 1.

Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine, Hefei, 230012, China.

Chromium is a harmful contaminant showing mutagenicity and carcinogenicity. Therefore, detection of chromium requires the development of low-cost and high-sensitivity sensors. Herein, blue-fluorescent carbon quantum dots were synthesized by one-step hydrothermal method from alkali-soluble polysaccharide, which is green source, cheap and easy to obtain, and has no pharmacological activity due to low water solubility. These carbon quantum dots exhibit good fluorescence stability, water solubility, anti-interference and low cytotoxicity, and can be specifically combined with the detection of Cr(VI) to form a non-fluorescent complex that causes fluorescence quenching, so they can be used as a label-free nanosensor. High-sensitivity detection of Cr(VI) was achieved through internal filtering and static quenching effects. The fluorescence quenching degree of carbon dots fluorescent probe showed a good linear relationship with Cr(VI) concentration in the range of 1-100 μM. The linear equation was / = 0.9942 + 0.01472 [Cr(VI)] (  = 0.9922), and the detection limit can be as low as 0.25 μM (S/N = 3), which has been successfully applied to Cr(VI) detection in actual water samples herein.
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http://dx.doi.org/10.1016/j.jpha.2021.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073324PMC
February 2022

Brain Functional Topology Alteration in Right Lateral Occipital Cortex Is Associated With Upper Extremity Motor Recovery.

Front Neurol 2022 3;13:780966. Epub 2022 Mar 3.

Rehabilitation Medicine Center, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Stroke is a chief cause of sudden brain damage that severely disrupts the whole-brain network. However, the potential mechanisms of motor recovery after stroke are uncertain and the prognosis of poststroke upper extremity recovery is still a challenge. This study investigated the global and local topological properties of the brain functional connectome in patients with subacute ischemic stroke and their associations with the clinical measurements. A total of 57 patients, consisting of 29 left-sided and 28 right-sided stroke patients, and 32 age- and gender-matched healthy controls (HCs) were recruited to undergo a resting-state functional magnetic resonance imaging (rs-fMRI) study; patients were also clinically evaluated with the Upper Extremity Fugl-Meyer Assessment (FMA_UE). The assessment was repeated at 15 weeks to assess upper extremity functional recovery for the patient remaining in the study (12 left- 20 right-sided stroke patients). Global graph topological disruption indices of stroke patients were significantly decreased compared with HCs but these indices were not significantly associated with FMA_UE. In addition, local brain network structure of stroke patients was altered, and the altered regions were dependent on the stroke site. Significant associations between local degree and motor performance and its recovery were observed in the right lateral occipital cortex (R LOC) in the right-sided stroke patients. Our findings suggested that brain functional topologies alterations in R LOC are promising as prognostic biomarkers for right-sided subacute stroke. This cortical area might be a potential target to be further validated for non-invasive brain stimulation treatment to improve poststroke upper extremity recovery.
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http://dx.doi.org/10.3389/fneur.2022.780966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927543PMC
March 2022

Chinese Herbal Extracts Exert Neuroprotective Effect in Alzheimer's Disease Mouse Through the Dopaminergic Synapse/Apoptosis Signaling Pathway.

Front Pharmacol 2022 28;13:817213. Epub 2022 Feb 28.

School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.

Alzheimer's disease (AD) as an age-related, irreversible neurodegenerative disease, characterized by cognitive dysfunction, has become progressively serious with a global rise in life expectancy. As the failure of drug elaboration, considerable research effort has been devoted to developing therapeutic strategies for treating AD. TCM is gaining attention as a potential treatment for AD. Blume, Willd., Cistanche deserticola Ma, Rehmannia lutinosa (Gaertn.)DC., Aiton, and Curcuma longa L. (GPCRAC) are all well-known Chinese herbs with neuroprotective benefits and are widely used in traditional Chinese decoction for AD therapy. However, the efficacy and further mechanisms of GPCRAC extracts in AD experimental models are still unclear. The purpose of this study was to investigate the synergistic protective efficacy of GPCRAC extracts (composed of extracts from these six Chinese medicines), and the protein targets mediated by GPCRAC extracts in treating AD. Scopolamine-induced cognitive impairment mouse model was established to determine the neuroprotective effects of GPCRAC extracts , as shown by behavioral tests and cerebral cholinergic function assays. To identify the potential molecular mechanism of GPCRAC extracts against AD, label-free quantitative proteomics coupled with tandem mass spectrometry (LC-MS/MS) were performed. The integrated bioinformatics analysis was applied to screen the core differentially expressed proteins in vital canonical pathways. Critical altered proteins were validated by qPCR and Western blotting. Administration of GPCRAC extracts significantly recovered scopolamine-induced cognitive impairment, as evidenced by the improved learning and memory ability, increased Ach content and ChAT activity, as well as decreased AchE activity in the hippocampus of mice. In total, 390 proteins with fold-change>1.2 or <0.83 and < 0.05 were identified as significant differentially expressed proteins, of which 110 were significantly up-regulated and 25 were significantly down-regulated between control and model group. By mapping the significantly regulated proteins, we identified five hub proteins: PPP2CA, Gsk3β, PP3CC, PRKACA, and BCL-2 that were associated with dopaminergic synapse and apoptosis signaling pathway, respectively. Western blotting and QPCR demonstrate that the expression levels of these core proteins could be significantly improved by the administration of GPCRAC extracts. These pathways and some of the identified proteins are implicated in AD pathogenesis. Administration of GPCRAC extracts was effective on alleviating scopolamine-induced cognitive impairment, which might be through modulation of dopaminergic synapse and apoptosis signaling pathway. Consequently, our quantitative proteome data obtained from scopolamine-treated model mice successfully characterized AD-related biological alterations and proposed novel protein biomarkers for AD.
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http://dx.doi.org/10.3389/fphar.2022.817213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918930PMC
February 2022

Characterization of a Recombinant Laccase B from MX2 and Its Application for Decolorization of Dyes.

Molecules 2022 Feb 27;27(5). Epub 2022 Feb 27.

The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.

is able to secrete laccase isoenzymes including constitutive and inducible forms, and has potential application for bioremediation of environmental pollutants. Here, an inducible group B laccase from MX2 was heterologously expressed in , and its yield reached 2.59 U/mL after 5 days of methanol inducing culture. The optimal pH and temperature of recombinant laccase (rLac1) to 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were 2.5 and 60 °C, respectively. Metal ions showed different effect on rLac1 which Mg, Cu, and K increased enzyme activity as their concentration increased, whereas Zn, Na, and Fe inhibited enzyme activity as their concentration increased. rLac1 showed good tolerance to organic solvents, and more than 42% of its initial activity remained in 10% organic solvents. Additionally, rLac1 exhibited a more efficient decolorization ability for remazol brilliant blue R (RBBR) than for acid red 1 (AR1), crystal violet (CV), and neutral red (NR). Molecular docking results showed RBBR has a stronger binding affinity with laccase than other dyes by interacting with substrate binding cavity of enzyme. The results indicated rLac1 may be a potential candidate for dye removal from textile wastewater.
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http://dx.doi.org/10.3390/molecules27051581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912056PMC
February 2022

Spatiotemporal Tracing of the Cellular Internalization Process of Rod-Shaped Nanostructures.

ACS Nano 2022 03 22;16(3):4059-4071. Epub 2022 Feb 22.

Laboratory of Controllable Nanopharmaceuticals, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P.R. China.

Endocytosis, as one of the main ways for nanostructures enter cells, is affected by several aspects, and shape is an especially critical aspect during the endocytosis of nanostructures. However, it has remained challenging to capture the dynamic internalization behaviors of rod-shaped nanostructures while also probing the mechanical aspects of the internalization. Here, using the atomic force microscopy-based force tracing technique, transmission electron microscopy, and molecular dynamic simulation, we mapped the detailed internalization behaviors of rod-shaped nanostructures with different aspect ratios at the single-particle level. We found that the gold nanorod is endocytosed in a noncontinuous and force-rebound rotation manner, herein named "intermittent rotation". The force tracing test indicated that the internalization force (∼81 pN, ∼108 pN, and ∼157 pN) and time (∼0.56 s, ∼0.66 s, and ∼1.14 s for a 12.10 nm × 11.96 nm gold nanosphere and 26.15 nm × 13.05 nm and 48.71 nm × 12.45 nm gold nanorods, respectively) are positively correlated with the aspect ratios. However, internalization speed is negatively correlated with internalization time, irrespective of the aspect ratio. Further, the energy analysis suggested that intermittent rotation from the horizontal to vertical direction can reduce energy dissipation during the internalization process. Thus, to overcome the energy barrier of internalization, the number and angle of rotation increases with aspect ratios. Our findings provide critical missing evidence of rod-shaped nanostructure's internalization, which is essential for fundamentally understanding the internalization mechanism in living cells.
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http://dx.doi.org/10.1021/acsnano.1c09684DOI Listing
March 2022

Eucommia, Cuscuta, and Drynaria Extracts Ameliorate Glucocorticoid-Induced Osteoporosis by Inhibiting Osteoclastogenesis Through PI3K/Akt Pathway.

Front Pharmacol 2021 4;12:772944. Epub 2022 Feb 4.

Shenzhen Research Institute, Beijing University of Chinese Medicine, Shenzhen, China.

Osteoporosis is one of the most common diseases in the world which resulted in heavy socioeconomic burden and a public health threat. Glucocorticoid-induced osteoporosis (GIO) is the most common secondary reason of osteoporosis. Therapeutic strategies using traditional Chinese medicine are under investigation for osteoporosis, with efforts to improve efficacy and clarify the mechanism. The combination of Eucommia, Cuscuta, and Drynaria is widely used in traditional Chinese decoction for osteoporosis treatment, but the experimental efficacy and mechanism are still unclear. Administration of E.C.D. extracts (Eucommia, Cuscuta, and Drynaria) in experimental GIO rats resulted in decreased urinal calcium, phosphorus loss, and decreased expression of RANKL, CTX in serum, increased serum calcium, phosphorus, and OPG level. E.C.D. extracts also improved bone density, structural integrity, and biomechanical function in experimental GIO rats. These finding were associated with E.C.D. extracts' treatment efficacy to GIO . The balance between osteoclast and osteoblast activity is essential for bone remodeling and bone related disease. The E.C.D. extracts inhibited Raw 264.7 cell differentiation to osteoclast . On the other hand, it promoted OPG expression of bone marrow mesenchymal stromal cells (MSCs) which can suppress the osteoclast genesis. E.C.D. extracts also increased the Wnt1 and Runx2 expression which are related to osteoblast formation. It also regulated the paracrine effect of MSC to inhibit osteoclast differentiation. The analysis of HPLC and comprehensive pharmacology identified the constituents of E.C.D. extracts and the potential osteoporosis-related targets mediated by E.C.D. extracts. The KEGG enrichment analysis suggested that PI3K/Akt pathway may be involved in the regulation osteoclast genesis by E.C.D. extracts and the result of Western blot of assays proved it. Collectively, these data demonstrate E.C.D. extracts can inhibit osteoclast differentiation to foster experimental osteoporosis both and and it may exert the function of inhibiting osteoclast differentiation through PI3K/Akt pathway.
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http://dx.doi.org/10.3389/fphar.2021.772944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855109PMC
February 2022

Dual-Facets Emissive Quantum-Dot Light-Emitting Diode Based on AZO Electrode.

Materials (Basel) 2022 Jan 19;15(3). Epub 2022 Jan 19.

School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.

We report on a green, dual emissive quantum-dot light-emitting diode (QLED) using alumina (Al)-doped ZnO (AZO) to adjust the band offset between the cathode and QD-emitting layers. The dual emissive QLED structure was designed by enhancing the efficient hole injection/transfer and slowing down the electron injection/transfer from AZO to the QD. The QLEDs presented a maximum luminance of 9450 cd/m, corresponding to a power efficiency of 15.7 lm/W, a current efficiency of 25.5 cd/A, as well as a turn-on voltage of 2.3 V. It is worth noting that the performance of the dual emissive QLED is comparable to that of a single emissive QLED. Therefore, there is a 1.3-fold enhancement in the performance of the QLED based on the AZO cathode due to the balanced charge injection/transfer.
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http://dx.doi.org/10.3390/ma15030740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836426PMC
January 2022

ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway.

Cell Death Discov 2022 Feb 8;8(1):51. Epub 2022 Feb 8.

Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, P. R. China.

Hair follicle-derived mesenchymal stem cells (HF-MSCs) show considerable therapeutic potential for liver cirrhosis (LC). To improve the effectiveness of naïve HF-MSC treatments on LC, we used bioinformatic tools to identify an exogenous gene targeting HSCs among the differentially expressed genes (DEGs) in LC to modify HF-MSCs. Extracellular matrix protein 1 (ECM1) was identified as a DEG that was significantly downregulated in the cirrhotic liver. Then, ECM1-overexpressing HF-MSCs (ECM1-HF-MSCs) were transplanted into mice with LC to explore the effectiveness and correlated mechanism of gene-overexpressing HF-MSCs on LC. The results showed that ECM1-HF-MSCs significantly improved liver function and liver pathological injury in LC after cell therapy relative to the other treatment groups. Moreover, we found that ECM1-HF-MSCs homed to the injured liver and expressed the hepatocyte-specific surface markers ALB, CK18, and AFP. In addition, hepatic stellate cell (HSC) activation was significantly inhibited in the cell treatment groups in vivo and in vitro, especially in the ECM1-HF-MSC group. Additionally, TGF-β/Smad signal inhibition was the most significant in the ECM1-HF-MSC group in vivo and in vitro. The findings indicate that the genetic modification of HF-MSCs with bioinformatic tools may provide a broad perspective for precision treatment of LC.
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http://dx.doi.org/10.1038/s41420-022-00846-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827057PMC
February 2022

Maturation Process and Characterization of a Novel Thermostable and Halotolerant Subtilisin-Like Protease with High Collagenolytic Activity but Low Gelatinolytic Activity.

Appl Environ Microbiol 2022 02 1;88(3):e0218421. Epub 2021 Dec 1.

State Key Laboratory of Virology, College of Life Sciences, Wuhan Universitygrid.49470.3e, Wuhan, China.

Enzymatic degradation of collagen is of great industrial and environmental significance; however, little is known about thermophile-derived collagenolytic proteases. Here, we report a novel collagenolytic protease (TSS) from thermophilic sp. WF146. The TSS precursor comprises a signal peptide, an N-terminal propeptide, a subtilisin-like catalytic domain, a β-jelly roll (βJR) domain, and a prepeptidase C-terminal (PPC) domain. The maturation of TSS involves a stepwise autoprocessing of the N-terminal propeptide and the PPC domain, and the βJR rather than the PPC domain is necessary for correct folding of the enzyme. Purified mature TSS displayed optimal activity at 70°C and pH 9.0, a half-life of 1.5 h at 75°C, and an increased thermostability as the NaCl concentration increased up to 4 M. TSS possesses an increased number of surface acidic residues and ion pairs, as well as four Ca-binding sites, which contribute to its high thermostability and halotolerance. At high temperatures, TSS exhibited high activity toward insoluble type I collagen and azocoll but showed a low gelatinolytic activity, with a strong preference for Arg and Gly at the P1 and P1' positions, respectively. Both the βJR and PPC domains could bind but not swell collagen, and thus facilitate TSS-mediated collagenolysis via improving the accessibility of the enzyme to the substrate. Additionally, TSS has the ability to efficiently degrade fish scale collagen at high temperatures. Proteolytic degradation of collagen at high temperatures has the advantages of increasing degradation efficiency and minimizing the risk of microbial contamination. Reports on thermostable collagenolytic proteases are limited, and their maturation and catalytic mechanisms remain to be elucidated. Our results demonstrate that the thermophile-derived TSS matures in an autocatalytic manner and represents one of the most thermostable collagenolytic proteases reported so far. At elevated temperatures, TSS prefers hydrolyzing insoluble heat-denatured collagen rather than gelatin, providing new insight into the mechanism of collagen degradation by thermostable collagenolytic proteases. Moreover, TSS has the potential to be used in recycling collagen-rich wastes such as fish scales.
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http://dx.doi.org/10.1128/aem.02184-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824211PMC
February 2022

Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.

J Ethnopharmacol 2022 May 29;289:115045. Epub 2022 Jan 29.

School of Life Sciences, Beijing University of Chinese Medicine, Liangxiang University Town, Fangshan District, Beijing, 102488, China; Shenzhen Research Institute, Beijing University of Chinese Medicine, Pingshan District, Shenzhen, 518118, China. Electronic address:

Ethnopharmacological Relevance: Goiter with hypothyroidism occurs in several thyroid diseases. Xiao-Luo-Wan (XLW), which contains Scrophularia ningpoensis Hemsl., Fritillaria thunbergii Miq. and Ostrea gigas Thunberg, has been used as an effective Chinese medicine for the treatment of goiters in China for hundreds of years. Based on clinical observations and experimental studies, XLW also exerts a certain effect on hypothyroidism. However, the therapeutic mechanism of XLW remains unclear.

Aim Of The Study: The present study aimed to investigate the therapeutic effect of XLW on propylthiouracil (PTU)-induced goiter with hypothyroidism in rats and to uncover the underlying molecular mechanism using ultra high-performance liquid chromatography-mass spectrometry (UPLC/MS), network pharmacology, and molecular docking simulations.

Materials And Methods: After successful modeling, the remaining rats were randomly divided into a model group, an Euthyrox group, an XLW group, and a control group. The corresponding drugs were given by gavage for four consecutive weeks. The growth status was monitored, the relative thyroid weight was calculated, and the total serum T3, T4, and TSH content were detected. Hematoxylin-eosin (H&E) staining was used to observe the pathological changes in the thyroid glands. The chemical components of the XLW were identified by UPLC/MS and the putative targets of XLW were predicted using multiple databases. We performed network pharmacology based on the intersection of goiter/hypothyroidism-related targets and XLW targets. Then, we performed KEGG pathway enrichment analysis, and key targets were further screened using protein-protein interaction (PPI) networks. Finally, molecular docking was used to predict the binding ability of XLW identified components and the key targets.

Results: XLW significantly increased the levels of T3 and T4, and reduced TSH, increased body weight, and decreased swollen thyroid glands in PTU-induced rats. XLW promoted the morphological recovery of thyroid follicles and epithelial cells. Twenty-one main chemical components of XLW were identified using UPLC/MS. 270 potential gene targets of XLW and 717 known targets of goiter/hypothyroidism disease were obtained by searching the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), Swiss Target Prediction, and UniProt databases. A total of 83 KEGG pathways were enriched with phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) and RAS signaling pathways. PPI analysis revealed nine key targets of kinase-protein kinase B (AKT) 1, interleukin (IL) 6, vascular endothelial growth factor A (VEGFA), tumor necrosis factor (TNF), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), epidermal growth factor receptor (EGFR), GTPase HRas (HRAS), matrix metalloproteinase (MMP) 9, and heat shock protein 90 alpha family class A member 1 (HSP90AA1). Molecular docking verified which drug components had good binding ability to key targets (all ≤5 kcal/mol).

Conclusion: For PTU-induced goiter with hypothyroidism in rats, XLW improves thyroid function, reduces goiter, increases body weight, and promotes the recovery of thyroid follicles and epithelial cells. The underlying molecular mechanism suggests that XLW may regulate thyroid hormone signaling by regulating the PI3K-AKT, RAS, and other signaling pathways. This study provides a pharmacological and biological basis for using XLW to treat goiter with hypothyroidism.
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http://dx.doi.org/10.1016/j.jep.2022.115045DOI Listing
May 2022

Tumor-Derived Extracellular Vesicles Regulate Cancer Progression in the Tumor Microenvironment.

Front Mol Biosci 2021 4;8:796385. Epub 2022 Jan 4.

College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.

Extracellular vesicles (EVs) are nanosized particles released by numerous kinds of cells, which are now increasingly considered as essential vehicles of cell-to-cell communication and biomarkers in disease diagnosis and treatment. They contain a variety of biomolecular components, including lipids, proteins and nucleic acids. These functional molecules can be transmitted between tumor cells and other stromal cells such as endothelial cells, fibroblasts and immune cells utilizing EVs. As a result, tumor-derived EVs can deliver molecules to remodel the tumor microenvironment, thereby influencing cancer progression. On the one hand, tumor-derived EVs reprogram functions of endothelial cells, promote cancer-associated fibroblasts transformation, induce resistance to therapy and inhibit the immune response to form a pro-tumorigenic environment. On the other hand, tumor-derived EVs stimulate the immune response to create an anti-tumoral environment. This article focuses on presenting a comprehensive and critical overview of the potential role of tumor-derived EVs-mediated communication in the tumor microenvironment.
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http://dx.doi.org/10.3389/fmolb.2021.796385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764126PMC
January 2022

Epimedium protects against dyszoospermia in mice with Pex3 knockout by exerting antioxidant effects and regulating the expression level of P16.

Cell Death Dis 2022 01 20;13(1):69. Epub 2022 Jan 20.

Reproductive Medicine Center of the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Oxidative stress (OS) is one of the primary factors leading to male infertility. Oral administration of antioxidants has thus far been found to significantly improve the quality of human sperm. Therefore, antioxidant treatment has become the consensus among international experts on male infertility. In this study, peroxisomal biogenesis factor 3 (Pex3)-knockout (KO, -/-) mice were used as a model to compare the efficacy of three types of traditional Chinese medicine (TCM) granules (Epimedium [YYH], Cuscuta [TSZ], and Rhodiola [HJT]) for male reproductive function rescue. YYH was revealed to be the best and exerted a rescue effect on Pex3-/- mice with spermatogenesis defects. In addition, YYH prominently reduced ROS levels in the testes, inhibited DNA oxidative damage in spermatogenic cells, promoted the proliferation of spermatogenic cells, and inhibited apoptosis in Pex3-/- male mice. Furthermore, the mechanism by which YYH ameliorated dyszoospermia was confirmed via the establishment of cyclin-dependent kinase inhibitor 2 A (P16Ink4a)-KO mice. Specifically, Pex3-/- mice produced elevated amounts of ROS, which damaged germ cell DNA and further activated the signaling pathway of the cell senescence regulatory protein P16-CDK6, resulting in cell cycle arrest and eventually contributing to spermatogenesis dysfunction. YYH supplementation partially corrected the associated phenotype in gene KO mice by affecting P16 expression levels, thus improving the reproductive outcome to a certain extent.
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http://dx.doi.org/10.1038/s41419-021-04435-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776794PMC
January 2022

Integrated Analysis of Angiogenesis-Mediated Tumor Immune Microenvironment Pattern in Hepatocellular Carcinoma (HCC) and a Novel Prognostic Model Construction to Predict Patient Outcome.

Med Sci Monit 2021 Dec 13;27:e934937. Epub 2021 Dec 13.

Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).

BACKGROUND Hepatocellular carcinoma (HCC) is a malignant tumor which is famous for its high heterogeneity and complex pathogenesis. Angiogenesis is an important driver of tumor progression and immune-suppressive microenvironment formation. MATERIAL AND METHODS A training set was acquired from the TCGA-LIHC cohort. An angiogenesis-active subtype was identified by consensus clustering analysis. The tumor subtype's immune microenvironment pattern was analyzed using quanTIseq. DEGs-mediated biology function was analyzed by enrichment analysis based on GO and KEGG. A prognostic model was constructed using LASSO Cox regression analysis and validated by 2 external datasets derived from GEO and ICGC. Quantitative real-time PCR assay was conducted to analyze CDCA8's expression status in the HCC line and normal liver cell line. RESULTS In HCC, patients with the angiogenesis-active subtype had a poor prognosis. Angiogenesis can shape the tumor microenvironment into high-M2 microphage infiltration and activity pattern. Here, we identified an angiogenesis-active HCC subtype and constructed an angiogenesis feature-based prognostic model to predict patient outcome. The external validation sets were enrolled to verify the accuracy of this model. CONCLUSIONS Our research demonstrated angiogenesis can confer the tumor immune-suppressive characteristic. We provide a robust method to evaluate the HCC's angiogenesis potential and help identify the angiogenesis-active subtype. Validation in the external validation cohort further confirmed the accuracy of our prognostic model.
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http://dx.doi.org/10.12659/MSM.934937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679551PMC
December 2021

Generation of 64-fs L-band stretched pulses from an all-fibre Er-doped laser.

Opt Express 2021 Oct;29(22):34892-34899

We demonstrate an L-band all-fibre erbium-doped laser mode locked by nonlinear polarisation rotation and working in the stretched-pulse regime. The use of a single segment of gain fibre with appropriate length and dispersion and a Brewster fibre grating optimised for the L band as an in-fibre polariser enables the generation of pulses at 1.59-μm central wavelength, which can be linearly compressed to 64-fs duration. Numerical simulations of the laser model support our experimental findings. Our laser design gives a route towards low-cost and low-complexity fibre-integrated laser sources for applications requiring L-band ultrashort pulses.
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http://dx.doi.org/10.1364/OE.434546DOI Listing
October 2021

Transient soliton dynamics in a mode-locked fiber laser: from stationary to pulsation.

Opt Lett 2021 Nov;46(22):5683-5686

We report the experimental observation of the transformation process from a stationary soliton to a pulsating soliton in a mode-locked fiber laser. The detailed soliton dynamics, including soliton parameter changings and sideband variation in the transition, are analyzed by the dispersive Fourier transformation technique. This Letter unveils the great enhancement of intracavity periodic perturbations resulting from the slight increase of soliton intensity induced soliton modulation instability, leading to the occurrence of parametric sidebands and multi-periodic pulsing instability. Our experimental results can shed some light on the intrinsic mechanism of soliton pulsation, as well as contribute to the study of soliton stability and laser dynamics.
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http://dx.doi.org/10.1364/OL.442740DOI Listing
November 2021

Vacuum sealing drainage combined with free anterolateral femoral skin flap grafting in 16 cases of pediatric soft tissue damage to the foot and ankle.

Transl Pediatr 2021 Oct;10(10):2489-2495

Department of Pediatrics, Tongde Hospital of Zhejiang Province, Hangzhou, China.

Background: To investigate the clinical experience and discuss the use of vacuum sealing drainage (VSD) combined with free anterolateral femoral flap grafting to treat pediatric foot and ankle soft tissue traumas.

Methods: This was a retrospective analysis of 16 pediatric patients with foot and ankle soft tissue traumas treated with free anterolateral femoral flap grafting from January 2015 to January 2018. After successful debridement and VSD, the clinical efficacy of the procedure was evaluated by observing parameters such as the color, shape, and texture of the flap on the receptor site.

Results: All patients were followed up for 23-32 months after surgery. Of the 16 patients, six had a flat flap appearance, while the remaining ten patients required flap repair surgery 6-9 months after the procedure. Eight of these ten patients had a satisfactory repair, and the remaining two patients had further repair surgery 9-10 months after the flap grafting. The postoperative results of all patients were satisfactory; no local infection, good flap survival, soft texture, glossy elasticity, and flat appearance. Statistical results showed only one case of local necrosis at the distal end of the flap, and the wound healed at stage II after redebridement and dressing change.

Conclusions: VSD combined with free anterolateral femoral flap grafting is an easy and reliable operation with a good prognosis and excellent clinical utility and feasibility for treating pediatric patients with foot and ankle soft tissue traumas.
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http://dx.doi.org/10.21037/tp-21-399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578767PMC
October 2021

Regulatory effects of miR-188-5p/XRCC5 on the progression of natural killer/T-cell lymphoma.

J BUON 2021 Sep-Oct;26(5):2033-2039

Department of Hematopathology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.

Purpose: Natural killer/T cell lymphoma (NKTCL) is a malignant condition. The molecular pathological mechanism of NKTCL has not been well studied. In this article we tried to study the role of microRNA-188-5p (miR-188-5p) in NKTCL.

Methods: The expression level of miR-188-5p and XRCC5 was examined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay and colony formation assay were used to assess the ability of cell proliferation. Dual luciferase reporter assay was used to examine the down-stream target of miR-188-5p. Western blotting was utilized to determine XRCC5 expression level.

Results: miR-188-5p was down-regulated in NKTCL. High expression of miR-188-5p accelerated cell proliferation. XRCC5 was one of the down-stream targets. Our data indicated that miR-188-5p suppressed NKTCL progression via regulating XRCC5 expression.

Conclusions: This research elucidated that miR-188-5p suppressed tumor progression in NKTCL by regulating XRCC5. Our data may provide more evidence in looking for novel therapeutic targets.
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February 2022

Renalase gene polymorphisms and plasma levels are associated with preeclampsia: a hospital-based study in the Chinese cohort.

Women Health 2021 Nov-Dec;61(10):957-967. Epub 2021 Nov 1.

Department of Obstetrics, Daqing People's Hospital, Daqing, Heilongjiang, China.

Preeclampsia (PE) is one of the major contributors to maternal and fetal mortality worldwide. Many host-related biomolecules regulate the pathophysiology of PE. The current study aims to examine the role of the renalase in PE manifestations. A total of 384 Chinese women consisting of subjects with normotensive pregnancy (n = 105), women with PE (n = 121), and healthy women (n = 158) were included in the study. Serum renalase level was measured in all subjects by ELISA. Renalase gene polymorphisms (rs10887800, rs2576178, and rs2296545) were genotyped by PCR-RFLP. The pregnant women had elevated renalase levels compared to healthy controls and subjects with PE. Renalase level was negatively correlated with systolic and diastolic blood pressure. Interestingly, renalase was positively correlated with the glomerular filtration rate. Prevalence of homozygous mutant (GG) and minor allele (G) for rs10887800 and rs2576178 renalase gene polymorphisms were significantly higher in PE patients compared to normotensive pregnant women and healthy controls. Furthermore, an association of G-G-C haplotype with susceptibility to PE was also noticed. A low level of renalase may be associated with an increased risk of PE during pregnancy. Renalase gene polymorphisms (rs10887800 and rs2576178) are correlated with serum renalase and are associated with predisposition to PE development in the Chinese cohort.
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http://dx.doi.org/10.1080/03630242.2021.1994512DOI Listing
February 2022

Composition and Protein Precipitation Capacity of Condensed Tannins in Purple Prairie Clover (.).

Front Plant Sci 2021 28;12:715282. Epub 2021 Sep 28.

Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, Canada.

This study aimed to determine the concentration and composition of condensed tannins (CT) in different tissues of purple prairie clover (PPC; Vent.) at different maturities and to determine their protein-precipitating capacity. The compositions of CT were elucidated after thiolysis with benzyl mercaptan followed by high-performance liquid-chromatography (HPLC) and H-C heteronuclear single quantum coherence (HSQC) NMR spectroscopy. The results indicated that PPC flowering heads contained the highest CT concentration. Purple prairie clover CT consisted mainly of epicatechin (EC) and epigallocatechin (EGC) subunits. CT in the leaves were composed of more EC and less EGC than CT in stems and flowering heads at both the early flowering (EF) and late flowering (LF) head stages. The mean degree of polymerization was the highest for CT in stems and increased with maturity. CT isolated from PPC leaves at the early flowering head stage exhibited the greatest biological activity in terms of protein precipitation. Overall, the CT in PPC were predominantly procyanidins and the concentration and composition varied among the plant tissues and with maturity.
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http://dx.doi.org/10.3389/fpls.2021.715282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509305PMC
September 2021

The Clinical Efficacy and Safety of 11 Commonly Used Treatment Strategies Improving Arrhythmia of CHD in China: A Network Meta-Analysis.

Front Pharmacol 2021 20;12:741716. Epub 2021 Sep 20.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Arrhythmia which as a common complication of CHD, has a high incidence. At present, more and more anti-arrhythmic drugs are used in clinical practice. However, which drug has the best efficacy and high safety is still unknown. Therefore, we decided to use NMA to solve this problem. We searched CNKI, Wanfang database, VIP database, Pubmed, Embase and Cochrane libraries, and collected all RCTs of arrhythmia of CHD, and used RevMan (5.3) and Stata (13.0) to carry out this NMA. The primary outcome indicator of this study is efficiency; the secondary outcome indicator is the incidence of adverse reactions. A total of 134 RCTs, 13,951 patients, and 11 treatment strategies were included in this NMA. The results show that all treatment strategies can effectively improve the arrhythmia of patients. Among them, PMA+AM, AM+AT, AM+WG have higher effective rates, and PMA+AM, WG+ME, SC+ME have better safety. The effectiveness and safety of the treatment strategies which combined TCM and chemical drugs, are significantly better than that of using chemical drugs alone. The treatment strategy of combination of multiple drugs usually has higher efficiency and safety. PMA+AM seems to be the most recommended treatment strategy. In addition, the rational combination of TCM and chemical drugs may provide potential benefit. : https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42021229693.
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http://dx.doi.org/10.3389/fphar.2021.741716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488134PMC
September 2021

Wavelength-tunable L-band mode-locked fiber laser using a long-period fiber grating.

Opt Express 2021 Aug;29(17):26332-26339

We demonstrate an L-band wavelength-tunable passively mode-locked fiber laser using a single long-period fiber grating (LPFG) as a narrow-band optical attenuator (NBOA). Through bending the LPFG, the central wavelength can be continuously tuned from 1582.02 to 1597.29 nm, while the output power only varies from 1.465 to 1.057 mW, approximately a rate of 22 µW/nm variation. This is the first time that LPFG is functioned as a NBOA in mode-locked fiber lasers, showing the great advantage of less impact on output power variation reduction. Besides, the total cavity length is 5.08 m, which is the shortest length yet reported in wavelength-tunable mode-locked fiber lasers. The wavelength tuning could also be realized at harmonic mode locking with tuning range of 14.69 nm under 5 harmonic.
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http://dx.doi.org/10.1364/OE.433298DOI Listing
August 2021

TP73-AS1 promotes malignant progression of NK/T cell lymphoma by regulating DKK1 methylation.

J BUON 2021 Jul-Aug;26(4):1530-1535

Department of Hematology, Guizhou Provincial People's Hospital, Guiyang, China.

Purpose: Long non-coding RNA (lncRNA) TP73-AS1 is abnormally expressed in multiple types of tumors, which is able to mediate tumor cell signals. This study aims to explore the role of TP73-AS1 in affecting biological functions of NK/T-cell lymphoma (NKTCL) and DKK1 methylation.

Methods: TP73-AS1 levels in peripheral blood of NKTCL patients and healthy volunteers was detected by quantitative real-time polymerase chain reaction (qRT-PCR). After knockdown of TP73-AS1, proliferative and migratory abilities in SNK-6 and HANK-1 cells were assessed by cell counting kit-8 (CCK-8) and Transwell assay, respectively. Regulatory effect of TP73-AS1 on DKK1 methylation in NKTCL cells was evaluated through methylation-specific PCR (MSP), dual-luciferase reporter assay and RNA Binding Protein Immunoprecipitation (RIP). Rescue experiments were conducted to further validate the interaction between TP73-AS1 and DKK1.

Results: TP73-AS1 level was higher in peripheral blood of NKTCL patients than that of healthy volunteers. Knockdown of TP73-AS1 in vitro weakened proliferative and migratory functions of NKTCL cells. TP73-AS1 induced methylation of DKK1 promoter through DNMT1/DNMT3, thus regulating NKTCL cell functions.

Conclusions: TP73-AS1 level was higher in peripheral blood of NKTCL patients. Through inducing methylation of DKK1 promoter, TP73-AS1 aggravates the malignant progression of NKTCL.
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January 2022
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