Publications by authors named "Qiang Zhou"

977 Publications

Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs.

Anim Nutr 2022 Jun 29;9:39-48. Epub 2021 Dec 29.

Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.

This experiment evaluated the impacts of essential oils (EO) and protease (PRO), independently or in combination, on growth performance, antioxidation, inflammation and intestinal function of weaned pigs. One hundred and sixty weaned pigs (21 d of age, BW of 6.74 ± 0.20 kg) were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen. Dietary treatments included the following: 1) control diet (CON), 2) CON with 300 mg/kg essential oils (EO), 3) CON with 500 mg/kg protease (PRO), 4) CON with 300 mg/kg essential oil and 500 mg/kg protease (EO + PRO). On d 8, one pig from each pen was selected for sampling. The remaining pigs were fed for an additional week and growth performance was monitored during this period. Dietary treatments had no marked effects ( > 0.05) on the growth performance of pigs. However, pigs receiving EO diet had higher ( < 0.05) serum glutathione peroxidase (GSH-Px) activity, and tended to decrease ( = 0.063) serum concentration of tumor necrosis factor-α (TNF-α). In addition, pigs receiving EO diet had higher ( < 0.05) abundances of phylum Actinobacteria, and genera , and lower ( < 0.05) phylum Bacteroidetes and genera in colonic digesta. Pigs receiving PRO diet decreased ( < 0.05) the serum concentration of malondialdehyde (MDA) and diamine oxidase activity, increased ( < 0.05) the villus height and the ratio of villus height to crypt depth in duodenum, increased sucrase activity in jejunal mucosa, and also increased the abundance of phylum Actinobacteria in colonic digesta. Furthermore, the synergistic effects of EO and PRO was observed ( < 0.05) for pigs with decreasing serum TNF-α concentration and increasing serum GSH-Px activity. Collectively, the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs. EO diet appeared to improve antioxidant activity and intestinal microbiota, while PRO diet improved intestinal morphology and digestive enzyme activity, and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs.
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http://dx.doi.org/10.1016/j.aninu.2021.12.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344314PMC
June 2022

Expression of Thioredoxin System Protein Induced by Silica in Rat Lung Tissue.

Biomed Environ Sci 2022 Jul;35(7):663-668

School of Public Health, North China University of Science and Technology, Tangshan 063210, Hebei, China;Xinxiang Medical University, Xinxiang 453003, Henan, China.

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http://dx.doi.org/10.3967/bes2022.087DOI Listing
July 2022

Metal-free synthesis of C2-quaternary indolinones by (NH)SO mediated oxidative dearomatization of indoles.

RSC Adv 2022 Jul 21;12(33):21022-21025. Epub 2022 Jul 21.

College of Chemistry and Life Science, Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional Molecules, Chengdu Normal University Chengdu 611130 P. R. China.

An efficient metal-free, (NH)SO mediated oxidative dearomatization of indoles for the construction of C2-quaternary indolinones was disclosed. A series of C2-quaternary indolinones derivatives with good functional group tolerance were obtained in moderate to excellent yields. This methodology provides an alternative approach for the direct generation of all-carbon quaternary centers at the C2 position of indoles. This catalytic approach represents a step-economic and convenient strategy for the oxidative dearomatization of indoles.
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http://dx.doi.org/10.1039/d2ra04191jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301541PMC
July 2022

A novel circular RNA, circIgfbp2, links neural plasticity and anxiety through targeting mitochondrial dysfunction and oxidative stress-induced synapse dysfunction after traumatic brain injury.

Mol Psychiatry 2022 Aug 2. Epub 2022 Aug 2.

Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Traumatic brain injury (TBI) can lead to different neurological and psychiatric disorders. Circular RNAs (circRNAs) are highly expressed in the nervous system and enriched in synapses; yet, the underlying role and mechanisms of circRNAs in neurological impairment and dysfunction are still not fully understood. In this study, we investigated the expression of circRNAs and their relation with neurological dysfunction after TBI. RNA-Seq was used to detect differentially expressed circRNAs in injured brain tissue, revealing that circIgfbp2 was significantly increased. Up-regulated hsa_circ_0058195, which was highly homologous to circIgfbp2, was further confirmed in the cerebral cortex specimens and serum samples of patients after TBI. Moreover, correlation analysis showed a positive correlation between hsa_circ_0058195 levels and the Self-Rating Anxiety Scale scores in these subjects. Furthermore, knockdown of circIgfbp2 in mice relieved anxiety-like behaviors and sleep disturbances induced by TBI. Knockdown of circIgfbp2 in HO treated HT22 cells alleviated mitochondrial dysfunction, while its overexpression reversed the process. Mechanistically, we discovered that circIgfbp2 targets miR-370-3p to regulate BACH1, and down-regulating BACH1 alleviated mitochondrial dysfunction and oxidative stress-induced synapse dysfunction. In conclusion, inhibition of circIgfbp2 alleviated mitochondrial dysfunction and oxidative stress-induced synapse dysfunction after TBI through the miR-370-3p/BACH1/HO-1 axis. Thus, circIgfbp2 might be a novel therapeutic target for anxiety and sleep disorders after TBI.
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http://dx.doi.org/10.1038/s41380-022-01711-7DOI Listing
August 2022

Efficacy of -3 Polyunsaturated Fatty Acids in Patients with Lung Cancer Undergoing Radiotherapy and Chemotherapy: A Meta-Analysis.

Int J Clin Pract 2022 8;2022:6564466. Epub 2022 Jul 8.

Department of Clinical Nutrition, West China Hospital of Sichuan University, Chengdu, China.

Background: Radiotherapy and chemotherapy in patients with lung cancer can lead to a series of problems such as malnutrition and inflammatory reaction. Some studies have shown that -3 polyunsaturated fatty acids (PUFAs) could improve malnutrition and regulate inflammatory reaction in these patients, but no relevant meta-analysis exists.

Methods: We systematically searched randomized controlled trials of -3 PUFAs in the adjuvant treatment of lung cancer in the PubMed, EMBASE, Cochrane Library, Web of Science, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), and Wanfang databases. Relevant outcomes were extracted, and we pooled standardized mean differences (SMDs) using a random or fixed-effects model. The risk of bias was evaluated according to the Cochrane Handbook (version 15.1). The quality of evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE).

Results: A total of 7 studies were included. The SMDs (95% CI) of body weight change, albumin change, energy intake, and protein intake at the end of intervention were 1.15 (0.50, 1.80), 0.60 (0.11, 1.09), 0.39 (-0.10, 0.89), and 0.27 (-0.04, 0.58), respectively. The SMDs (95% CI) of CRP change and TNF- change were -3.44 (-6.15, -0.73) and -1.63 (-2.53, -0.73), respectively.

Conclusions: -3 PUFAs can improve nutritional status and regulate indicators of inflammation in patients with lung cancer undergoing radiotherapy and chemotherapy. This study was registered in the PROSPERO (registration number: CRD42022307699).
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http://dx.doi.org/10.1155/2022/6564466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303080PMC
August 2022

The genome of the rice planthopper egg parasitoid wasps Anagrus nilaparvatae casts light on the chemo- and mechanosensation in parasitism.

BMC Genomics 2022 Jul 28;23(1):541. Epub 2022 Jul 28.

State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.

Background: Mymaridae is an ancient insect group and is a basal lineage of the superfamily Chalcidoidea. Species of Mymaridae have great potential for biological control. Anagrus nilaparvatae, a representative species of Mymaridae, is ideal for controlling rice planthopper due to its high rate of parasitism and ability to find hosts efficiently in paddy ridges and fields.

Results: Using both PacBio single-molecule real-time and Illumina sequencing, we sequenced and assembled the whole genome of A. nilaparvatae, a first for the family Mymaridae. The assembly consists of 394 scaffolds, totaling 488.8 Mb. The assembly is of high continuity and completeness, indicated by the N50 value of 25.4 Mb and 98.2% mapping rate of Benchmarking Universal Single-Copy Orthologs. In total, 16,894 protein-coding genes in the genome were annotated. A phylogenomic tree constructed for A. nilaparvatae and other 12 species of Hymenoptera confirmed that the family Mymaridae is sister to all remaining chalcidoids. The divergence time between A. nilaparvatae and the other seven Chalcidoidea species was dated at ~ 126.9 Mya. Chemoreceptor and mechanoreceptor genes are important in explaining parasitic behavior. We identified 17 odorant binding proteins, 11 chemosensory proteins, four Niemann-Pick type C2 proteins, 88 olfactory receptors, 12 gustatory receptors, 22 ionotropic receptors and 13 sensory neuron membrane proteins in the genome of A. nilaparvatae, which are associated with the chemosensory functions. Strikingly, there is only one pickpocket receptors and nine transient receptor potential genes in the genome that have a mechanosensory function.

Conclusions: We obtained a high-quality genome assembly for A. nilaparvatae using PacBio single-molecule real-time sequencing, which provides phylogenomic insights for its evolutionary history. The small numbers of chemo- and mechanosensory genes in A. nilaparvatae indicate the species-specific host detection and oviposition behavior of A. nilaparvatae might be regulated by relatively simple molecular pathways.
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http://dx.doi.org/10.1186/s12864-022-08656-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331105PMC
July 2022

The Efficacy of Remifentanil Combined with Propofol in Craniotomy for Tumor Was Evaluated by Wake Quality, Hemodynamics, and Adverse Reactions.

Biomed Res Int 2022 18;2022:4861043. Epub 2022 Jul 18.

Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300350, China.

In order to investigate the effect of remifentanil combined with propofol on awakening of craniotomy for tumor, a retrospective analysis is conducted. 86 patients who underwent craniotomy for brain tumor in our hospital from July 2020 to December 2021 are chosen to observe the quality of awakening, hemodynamic parameters, and the occurrence of adverse reactions. All patients are divided into group A ( = 43) and group B ( = 43) according to the use of anesthesia drugs. The intraoperative awakening quality and the hemodynamic parameters during different periods of the two groups are compared. The experimental results show that the incidence of postoperative adverse reactions in group B is significantly lower than that in group A ( < 0.05). It is clearly evident that remifentanil combined with protocol has good intraoperative wake-up effect in craniotomy for tumor and maintain the hemodynamic stability of patients. Also, it can obtain high wake-up quality and effectively reduce postoperative adverse reactions.
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http://dx.doi.org/10.1155/2022/4861043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314166PMC
July 2022

Enhanced arsenic removal by reusable hexagonal CeO/FeO nanosheets with exposed (0001) facet.

Sci Total Environ 2022 Jul 20;847:157490. Epub 2022 Jul 20.

Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing, Jiangsu 210096, China. Electronic address:

Arsenite in wastewater has caused increasing concern because of high toxicity and mobility. Iron oxides are widely available and regarded as effective adsorbents for arsenic. However, conventional iron oxides usually are only effective for arsenate (As(V)) adsorption by complexation, but not for As(III) adsorption because of their poor catalytic oxidation activities, which greatly limits arsenic removal efficiency. In this study, a uniform hexagonal FeCe bimetal oxide nanosheets (FeCeO) enclosed by high active (0001) planes was synthesized by a solvothermal method to improve the catalytic activity of FeO. The experimental results showed that adsorption capacity of FeCeO reached 61.1 mg/g for arsenic and 70 % of that at equilibrium was achieved in <10 min. Based on characterization analyses and density functional theory simulation, the new insight in oxidation and complexation mechanism of arsenic was proposed. Firstly, As(III) was adsorbed to adsorbent surface by forming stable structure of Ce-O-As or Fe-O-As, and then converted into As(V) by dissolved oxygen under the catalysis of (0001) planes densely distributed on FeO and CeO surfaces. The formed As(V) species were bound on FeCeO surface by forming bidentate and monodentate surface complexes. Finally, the safety of As-containing solution treated with FeCeO was well proved by the zebrafish embryo developmental toxicity tests.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157490DOI Listing
July 2022

VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types.

PLoS One 2022 21;17(7):e0269818. Epub 2022 Jul 21.

Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, College of Medicine, Chicago, Illinois, United States of America.

Purpose: We have previously reported that VEGF-B is more potent than VEGF-A in mediating corneal nerve growth in vitro and in vivo, and this stimulation of nerve growth appears to be different from stimulation of angiogenesis by these same ligands, at least in part due to differences in VEGF receptor activation. VEGF signaling may be modulated by a number of factors including receptor number or the formation of receptor hetero- vs. homodimers. In endothelial cells, VEGF receptor heterodimer (VEGR1/R2) activation after ligand binding and subsequent phosphorylation alters the activation of downstream signaling cascades. However, our understanding of these processes in neuronal cell types remains unclear. The purpose of this study was to identify the presence and distribution of VEGF Receptor-Ligand interactions in neuronal cells as compared to endothelial cells.

Methods: PC12 (rat neuronal cell line), MAEC (mouse aortic endothelial cell line), MVEC (mouse venous endothelial cell line) and HUVEC (human umbilical venous endothelial cell line; control group) were used. Cells were acutely stimulated either with VEGF-A (50 ng/μL) or VEGF-B (50 ng/μL) or "vehicle" (PBS; control group). We also isolated mouse trigeminal ganglion cells from thy1-YFP neurofluorescent mice. After treatment, cells were used as follows: (i) One group was fixed in 4% paraformaldehyde and processed for VEGFR1 and VEGFR2 immunostaining and visualized using confocal fluorescence microscopy and Total Internal Reflection (TIRF) microscopy; (ii) the second group was harvested in cell lysis buffer (containing anti-protease / anti-phosphatase cocktail), lysed and processed for immunoprecipitation (IP; Thermo Fisher IP kit) and immunoblotting (IB; LI-COR® Systems). Immunoprecipitated proteins were probed either with anti-VEGFR1 or anti-VEGFR2 IgG antibodies to evaluate VEGFR1-R2-heterodimerization; (iii) a third group of cells was also processed for Duolink Proximity Ligation Assay (PLA; Sigma) to assess the presence and distribution of VEGF-receptor homo- and heterodimers in neuronal and endothelial cells.

Results: TIRF and fluorescence confocal microscopy revealed the presence of VEGFR1 co-localized with VEGFR2 in endothelial and PC12 neuronal cells. Cell lysates immunoprecipitated with anti-VEGFR1 further validated the existence of VEGFR1-R2 heterodimers in PC12 neuronal cells. Neuronal cells showed higher levels of VEGFR1-R2 heterodimers as compared to endothelial cells whereas endothelial cells showed higher VEGFR2-R2 homodimers compared to neuronal cells as demonstrated by Duolink PLA. Levels of VEGFR1-R1 homodimers were very low in neuronal and endothelial cells.

Conclusions: Differences in VEGF Receptor homo- and heterodimer distribution may explain the differential role of VEGF ligands in neuronal versus endothelial cell types. This may in turn influence VEGF activity and regulation of neuronal cell homeostasis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0269818PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302817PMC
July 2022

A randomized controlled trial to evaluate efficacy and safety of early conversion to a low-dose calcineurin inhibitor combined with sirolimus in renal transplant patients.

Chin Med J (Engl) 2022 Jul 25. Epub 2022 Jul 25.

Department of Urology, Capital Medical University Beijing Chaoyang Hospital, Beijing 100020, China.

Background: The calcineurin inhibitor (CNI)-based immune maintenance regimen that is commonly used after renal transplantation has greatly improved early graft survival after transplantation; however, the long-term prognosis of grafts has not been significantly improved. The nephrotoxicity of CNI drugs is one of the main risk factors for the poor long-term prognosis of grafts. Sirolimus (SRL) has been employed as an immunosuppressant in clinical practice for over 20 years and has been found to have no nephrotoxic effects on grafts. Presently, the regimen and timing of SRL application after renal transplantation vary, and clinical data are scarce. Multicenter prospective randomized controlled studies are particularly rare. This study aims to investigate the effects of early conversion to a low-dose CNI combined with SRL on the long-term prognosis of renal transplantation.

Methods: Patients who receive four weeks of a standard regimen with CNI + mycophenolic acid (MPA) + glucocorticoid after renal transplantation in multiple transplant centers across China will be included in this study. At week 5, after the operation, patients in the experimental group will receive an additional administration of SRL, a reduction in the CNI drug doses, withdrawal of MPA medication, and maintenance of glucocorticoids. In addition, patients in the control group will receive the maintained standard of care. The patients' vital signs, routine blood tests, routine urine tests, blood biochemistry, serum creatinine, BK virus (BKV)/ cytomegalovirus (CMV), and trough concentrations of CNI drugs and SRL at the baseline and weeks 12, 24, 36, 48, 72, and 104 after conversion will be recorded. Patient survival, graft survival, and estimated glomerular filtration rate will be calculated, and concomitant medications and adverse events will also be recorded.

Conclusion: The study data will be utilized to evaluate the efficacy and safety of early conversion to low-dose CNIs combined with SRL in renal transplant patients.

Trial Registration: Chinese Clinical Trial Registry, ChiCTR1800017277.
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http://dx.doi.org/10.1097/CM9.0000000000001866DOI Listing
July 2022

Complete chloroplast genome sequences of Ying Liu & Q.E. Yang (Asteraceae).

Mitochondrial DNA B Resour 2022 12;7(7):1280-1281. Epub 2022 Jul 12.

Key Laboratory of Plant Resources Conservation and Utilization, Jishou University, Jishou, Hunan, China.

In this study, we assembled and described the complete chloroplast genome (cp) of Ying Liu & Q.E. Yang (Asteraceae) for the first time. The cp genome is 151,315 bp in length and exhibits a typical quadripartite structure, consisting of a large single-copy (LSC) region of 83,445 bp, a small single-copy (SSC) region of 18,172 bp, and a pair of inverted repeat regions (IRs) of 24,849 bp. A total of 133 genes were annotated, including 88 protein-coding genes (one pseudo gene), 37 tRNA genes (tRNAs), and eight ribosomal RNA genes (rRNAs). Further, the phylogenetic analysis based on eight complete chloroplast genomes indicates that is shown to be a sister to . The complete chloroplast genome of provides significant molecular markers for the studies on phylogeny and species identification of this genus.
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http://dx.doi.org/10.1080/23802359.2022.2097026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278445PMC
July 2022

Spheroid Formation Enhances the Regenerative Capacity of Nucleus Pulposus Cells via Regulating N-CDH and ITGβ1 Interaction.

Int J Biol Sci 2022 21;18(9):3676-3696. Epub 2022 May 21.

Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.

Nucleus pulposus (NP) degeneration is the core pathological change of intervertebral disc (IVD) degenerative diseases, but currently, no effective therapy is available. With the rapid development of biomaterials and tissue engineering in recent years, biomaterial-assisted cell transplantation becomes a promising therapy for IVD degeneration. However, the application is severely limited by the weak biological characteristics of NP cells (NPCs), such as a moderate proliferation ability, weak self-renewal capacity, and minimal extracellular matrix (ECM) synthesis capacity, caused by the current inappropriate cell seeding or grafting methods. Here, we developed a three-dimensional (3D) spheroidizing culture method to construct NPC spheroids and investigated repair and regeneration potential of these spheroids in vitro and in vivo. The in vitro biological characteristics (including cell viability and proliferation), and in vivo functions (including anti-degeneration potential and ability to induce tissue repair) of NPC spheroids and monolayer-cultured NPCs were compared. Furthermore, an RNA-seq-based transcriptome analysis and a series of function experiments were performed to elucidate the potential mechanisms of their differences that were involved in the tissue regeneration process. NPC spheroids exhibited obviously superior self-renewal and ECM synthesis capacities compared to monolayers of NPCs in vitro. In vivo, NPC spheroids generated more functional ECM components, primarily aggrecan (ACAN) and collagen type II (Col2), and markedly promoted NP regeneration in the disc degeneration model induced by partial NP excision. Additionally, the biological characteristics and functions of NPC spheroids were to some extent regulated by the interaction of N-cadherin (N-CDH) and Integrinβ1 (ITGβ1), two key mechanosensing ECM-receptors expressed on NPCs. The NPC spheroidizing culture method is beneficial for cell renewal and the generation of functional ECM in NP tissue. The molecular mechanism involved in this regeneration process is closely associated with the regulation of the N-CDH and ITGβ1 interaction-mediated ECM homeostesis. Moreover, the strategy of hydrogel-assisted NPC spheroids transplantation may potentially be used in the future treatment of IVD degeneration.
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http://dx.doi.org/10.7150/ijbs.70903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254483PMC
July 2022

Erratum: EFEMP2 suppresses epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human bladder cancer: Erratum.

Int J Biol Sci 2022 27;18(9):3798-3799. Epub 2022 May 27.

Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.

[This corrects the article DOI: 10.7150/ijbs.35541.].
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http://dx.doi.org/10.7150/ijbs.75156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254484PMC
May 2022

Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states.

Nat Commun 2022 Jul 8;13(1):3957. Epub 2022 Jul 8.

Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, 310024, Zhejiang Province, China.

Sodium-Potassium Pump (Na/K-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic sodium ions for two extracellular potassium ions through alternating changes between the E1 and E2 states. Hitherto, several steps remained unknown during the complete working cycle of NKA. Here, we report cryo-electron microscopy (cryo-EM) structures of recombinant human NKA (hNKA) in three distinct states at 2.7-3.2 Å resolution, representing the E1·3Na and E1·3Na·ATP states with cytosolic gates open and the basic E2·[2K] state, respectively. This work provides the insights into the cytoplasmic Na entrance pathway and the mechanism of cytoplasmic gate closure coupled with ATP hydrolysis, filling crucial gaps in the structural elucidation of the Post-Albers cycle of NKA.
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http://dx.doi.org/10.1038/s41467-022-31602-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270386PMC
July 2022

Tuning the nonlinearity of graphene mechanical resonators by Joule heating.

J Phys Condens Matter 2022 Jul 15;34(37). Epub 2022 Jul 15.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

As an inherent property of the device itself, nonlinearity in micro-/nano- electromechanical resonators is difficult to eliminate, and it has shown a wide range of applications in basic research, sensing and other fields. While many application scenarios require tunability of the nonlinearity, inherent nonlinearity of a mechanical resonator is difficult to be changed. Here, we report the experimental observation of a Joule heating induced tuning effect on the nonlinearity of graphene mechanical resonators. We fabricated multiple graphene mechanical resonators and detected their resonant properties by an optical interference method. The mechanical vibration of the resonators will enter from the linear to the nonlinear intervals if we enhance the external driving power to a certain value. We found that at a fixed drive power, the nonlinearity of a mechanical resonator can be tuned by applying a dc bias current on the resonator itself. The tuning mechanism could be explained by the nonlinear amplitude-frequency dependence theory. Our results may provide a research platform for the study of mechanical nonlinearity by using atomic-thin layer materials.
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http://dx.doi.org/10.1088/1361-648X/ac7dd7DOI Listing
July 2022

Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2.

Nat Microbiol 2022 07 30;7(7):1063-1074. Epub 2022 Jun 30.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Institute of Infectious Diseases and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.

Frequent outbreaks of coronaviruses underscore the need for antivirals and vaccines that can counter a broad range of coronavirus types. We isolated a human antibody named 76E1 from a COVID-19 convalescent patient, and report that it has broad-range neutralizing activity against multiple α- and β-coronaviruses, including the SARS-CoV-2 variants. 76E1 also binds its epitope in peptides from γ- and δ-coronaviruses. 76E1 cross-protects against SARS-CoV-2 and HCoV-OC43 infection in both prophylactic and therapeutic murine animal models. Structural and functional studies revealed that 76E1 targets a unique epitope within the spike protein that comprises the highly conserved S2' site and the fusion peptide. The epitope that 76E1 binds is partially buried in the structure of the SARS-CoV-2 spike trimer in the prefusion state, but is exposed when the spike protein binds to ACE2. This observation suggests that 76E1 binds to the epitope at an intermediate state of the spike trimer during the transition from the prefusion to the postfusion state, thereby blocking membrane fusion and viral entry. We hope that the identification of this crucial epitope, which can be recognized by 76E1, will guide epitope-based design of next-generation pan-coronavirus vaccines and antivirals.
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http://dx.doi.org/10.1038/s41564-022-01155-3DOI Listing
July 2022

A Novel Approach Combined with MIPO Technique for the Treatment of Type C Pilon Fractures.

Oxid Med Cell Longev 2022 14;2022:7427255. Epub 2022 Jun 14.

Department of Orthopedics, Chongqing University Three Gorges Hospital, Chongqing 404000, China.

Objective: Type C fracture is a complete intra-articular fracture, and the mainstay of treatment remains open reduction and internal fixation. The purpose of the study is to observe the clinical effect of an anterior ankle C approach (ankle-C) combined with minimal invasive plate osteosystems (MIPO) for tibial pilon fractures (AO/OTA 43C, combined with fibula fractures).

Methods: A retrospective comparative analysis was performed on the clinical data of 33 patients with C-type pilon fractures (combined fibula fractures) admitted to our department from July 2018 to July 2021, including 12 cases treated with ankle-C (a-C) approach and 21 cases with conventional approach (including combined approach). All patients were followed up for over 6 months. Visual Analogue Scale (VAS), AOFAS Ankle-Hindfoot Scale (AOFAS-AHS), wound healing time, fracture healing time, and complications were used to evaluate the clinical efficacy.

Results: The scores of VAS and AOFAS in the a-C group scored better than the conventional group ( < 0.05), especially in the extent of limited range of motion (LROM) of ankle dorsiflexion-plantarflexion in 1 month after operation and at the last follow-up ( < 0.01). Bone healing was achieved in both groups 6 months after operation, with no implant exposure or infection. Among them, 4 cases in the conventional approach group had wound healing time exceeding 2 weeks.

Conclusions: For type C pilon fractures (combined with fibula fractures), ankle-C approach combined with MIPO technique has certain advantages in ankle function recovery and soft tissue repair, which provides an alternative for the treatment of type C pilon fractures.
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http://dx.doi.org/10.1155/2022/7427255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213148PMC
June 2022

A Facile Fabrication of Lysosome-Targeting pH Fluorescent Nanosensor Based on PEGylated Polyester Block Copolymer.

Polymers (Basel) 2022 Jun 15;14(12). Epub 2022 Jun 15.

CAS Key Laboratory of Soft Matter Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China.

A novel lysosome-targeting PEGylated polyester-based fluorescent pH nanosensor is fabricated by the combination of ring-opening copolymerization (ROCOP), side-group modification and subsequent self-assembly. First, a key target amphiphilic copolymer carrier for rhodamine (Rh) pH indicator is synthesized in a facile manner by the ROCOP of phthalic anhydride with allyl glycidyl ether using mPEG-OH and -BuP/EtB as the macroinitiator and binary catalyst, respectively. Subsequently, Rh moieties are covalently attached on the polymer chain with controllable grafting degree via an efficient thiol-ene click reaction. Concurrently, the effect of catalyst systems and reaction conditions on the catalytic copolymerization performance is presented, and the quantitative introduction of Rh is described in detail. Owing to its amphiphilic characteristics, the rhodamine-functionalized polyester-based block copolymer can self-assemble into micelles. With the covalent incorporation of Rh moieties, the as-formed micelles exhibit excellent absorption and fluorescence-responsive sensitivity and selectivity towards H in the presence of various metal cations. Moreover, the as-prepared micelles with favorable water dispersibility, good pH sensitivity and excellent biocompatibility also display appreciable cell-membrane permeability, staining ability and pH detection capability for lysosomes in living cells. This work provides a new strategy for the facile synthesis of novel biocompatible polymeric fluorescent pH nanosensors for the fluorescence imaging of lysosomal pH changes.
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http://dx.doi.org/10.3390/polym14122420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231249PMC
June 2022

Integrative Analyses of Transcriptomes and Metabolomes Reveal Associated Genes and Metabolites with Flowering Regulation in Common Vetch ( L.).

Int J Mol Sci 2022 Jun 19;23(12). Epub 2022 Jun 19.

State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.

As an important source of protein for livestock and human consumption, is cultivated worldwide, but its seed production is hampered at high altitudes because of the short frost-free period. Flowering represents the transition from a vegetative to a reproductive period, and early flowering benefits plant seed production at high altitudes. However, the molecular mechanisms of flowering regulation in remain elusive. In the present study, two accessions with different flowering characteristics were used: Lan3 (early-flowering) was cultivated by our laboratory, and 503 (late-flowering) was selected from 222 accessions after three years of field experiments. The shoot samples (shoot tip length = 10 cm) of these two accessions were collected 63, 70, and 77 days after sowing, and the molecular regulatory mechanism of the flowering process was identified by integrative analyses of the transcriptomes and metabolomes. Kyoto Encyclopedia of Genes and Genomes enrichment showed that the synthesis and signal transduction of plant hormone pathways were the most enriched pathways in 4274 differentially expressed genes (DEGs) and in 259 differential metabolites between Lan3 and 503. Moreover, the contents of three metabolites related to salicylic acid biosynthesis and the transcription levels of two DEGs related to salicylic acid signal transduction in Lan3 were higher than those in 503. Further verification in various accessions indicated that salicylic acid metabolism may be involved in the flowering regulation process of . These findings provide valuable information for understanding the flowering mechanism and for promoting breeding research in .
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http://dx.doi.org/10.3390/ijms23126818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224626PMC
June 2022

Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by H-NMR Spectroscopy.

Animals (Basel) 2022 Jun 14;12(12). Epub 2022 Jun 14.

Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy.

As an assisted breeding technique, artificial insemination has become the main effective practical approach in the captive breeding programs of giant panda worldwide. The composition of seminal plasma plays an important role in the success of breeding. The present work is the first attempt to characterize, by proton magnetic resonance spectroscopy (H-NMR), the metabolome of healthy giant panda seminal plasma. A total of 35 molecules were quantified, with the concentration of 2,3-butanediol being significantly different between individuals younger than 8 years and older than 13 years, and other distinct age-related trends were highlighted by a multivariate analysis. Isopropanol's concentration was significantly linked to estrus stages. Besides, the variations in the metabolome's profile during storage were also evaluated. This study may serve as a reference for further research wishing to shed light on the biological mechanisms affecting giant panda sperm's overall quality and may ultimately lead to novel approaches to giant panda artificial insemination.
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http://dx.doi.org/10.3390/ani12121536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219455PMC
June 2022

Nanotechnology-Based Diagnostic and Therapeutic Strategies for Neuroblastoma.

Front Pharmacol 2022 2;13:908713. Epub 2022 Jun 2.

Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou University, Zhengzhou, China.

Neuroblastoma (NB), as the most common extracranial solid tumor in childhood, is one of the critical culprits affecting children's health. Given the heterogeneity and invisibility of NB tumors, the existing diagnostic and therapeutic approaches are inadequate and ineffective in early screening and prognostic improvement. With the rapid innovation and development of nanotechnology, nanomedicines have attracted widespread attention in the field of oncology research for their excellent physiological and chemical properties. In this review, we first explored the current common obstacles in the diagnosis and treatment of NB. Then we comprehensively summarized the advancements in nanotechnology-based multimodal synergistic diagnosis and treatment of NB and elucidate the underlying mechanisms. In addition, a discussion of the pending challenges in biocompatibility and toxicity of nanomedicine was conducted. Finally, we described the development and application status of nanomaterials against some of the recognized targets in the field of NB research, and pointed out prospects for nanomedicine-based precision diagnosis and therapy of NB.
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http://dx.doi.org/10.3389/fphar.2022.908713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201105PMC
June 2022

Rational exploration of fold atlas for human solute carrier proteins.

Structure 2022 Jun 1. Epub 2022 Jun 1.

Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China; Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China; Westlake AI Therapeutics Lab, Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China. Electronic address:

The solute carrier (SLC) superfamily is the largest group of proteins responsible for the transmembrane transport of substances in human cells. It includes more than 400 members that are organized into 65 families according to their physiological function and sequence similarity. Different families of SLCs can adopt the same or different folds that determine the mechanism and reflect the evolutionary relationship between SLC members. Analysis of structural data in the literature before this work showed 13 different folds in the SLC superfamily covering 40 families and 343 members. To further study their mechanism, we systematically explored the SLC superfamily to look for more folds. Based on our results, at least three new folds are found for the SLC superfamily, one of which is in the choline-like transporter family (SLC44) and has been experimentally verified. Our work has laid a foundation and provided important insights for the systematic and comprehensive study of the structure and function of SLC.
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http://dx.doi.org/10.1016/j.str.2022.05.015DOI Listing
June 2022

Direct Electro Plasmonic and Optic Modulation via a Nanoscopic Electron Reservoir.

Phys Rev Lett 2022 May;128(21):217401

School of Physics and Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan 430074, China and Institute for Quantum Science and Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan 430074, China.

Direct electrical tuning of localized plasmons at optical frequencies boasts the fascinating prospects of being ultrafast and energy efficient and having an ultrasmall footprint. However, the prospects are obscured by the grand challenge of effectively modulating the very large number of conduction electrons in three-dimensional metallic structures. Here we propose the concept of nanoscopic electron reservoir (NER) for direct electro plasmonic and electro-optic modulation. A NER is a few-to-ten-nanometer size metal feature on a metal host and supports a localized plasmon mode. We provide a general guideline to construct highly electrically susceptible NERs and theoretically demonstrate pronounced direct electrical tuning of the plasmon mode by exploiting the nonclassical effects of conduction electrons. Moreover, we show the electro-plasmonic tuning can be efficiently translated into modulation of optical scattering by utilizing the antenna effect of the metal host for the NER. Our work extends the landscape of electro plasmonic modulation and opens appealing new opportunities for quantum plasmonics.
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http://dx.doi.org/10.1103/PhysRevLett.128.217401DOI Listing
May 2022

Methylation of arsenic differs with substrates in Arcticibacter tournemirensis R1 from an As-contaminated paddy soil.

Sci Total Environ 2022 Sep 6;838(Pt 4):156527. Epub 2022 Jun 6.

Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, United States. Electronic address:

Arsenic can be methylated by arsenite (As(III)) S-adenosylmethionine methyltransferases (ArsMs) among various kingdoms of life. The intermediate product methylarsenite (MAs(III)) is highly toxic and can be utilized as an antibiotic by some microbes. ArsM gene is widely distributed in the members of every kingdom from bacteria to humans and displays a high diversity of sequence. Based on arsenic methylating capacity, ArsM proteins can be divided into two phylogenetically distinct clades (Groups 1 and 2). In this study, we show that Arcticibacter tournemirensis R1 isolated from arsenic contaminated paddy soil is resistant to both As(III) and MAs(III), but exhibits different methylation activities for As(III) and MAs(III). The A. tournemirensis R1 shows low As(III) methylation activity and produces an unknown arsenic compound. In contrast, it shows high methylation activity with MAs(III), with the main product of dimethylarsenate (DMAs(V)). An AtarsM gene is found in ars operon of A. tournemirensis R1 genome and is regulated by an atypical transcriptional repressor ArsR. Expressed in Escherichia coli AtArsM confers resistance to As(III) and MAs(III). Both in vivo and in vitro assays show that AtArsM methylates As(III) and MAs(III) to dimethyl- and trimethyl‑arsenicals. AtArsM has four conserved cysteine residues, which are present in most ArsMs and can be classified into phylogenetic group 2 family, producing trimethylated arsenic metabolites. The high arsenic methylation and volatilization activity of AtArsM provides a potential strategy for arsenic bioremediation. The methylation activity differs with As(III) and MAs(III) in A. tournemirensis R1 indicates that there may have different detoxification mechanisms for As(III) and MAs(III), which are worth investigating in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156527DOI Listing
September 2022

Structure of the cytoplasmic ring of the nuclear pore complex.

Science 2022 06 10;376(6598):eabl8280. Epub 2022 Jun 10.

Westlake Laboratory of Life Sciences and Biomedicine, Westlake University, 310024 Hangzhou, China.

INTRODUCTION The nuclear pore complex (NPC) resides on the nuclear envelope (NE) and mediates nucleocytoplasmic cargo transport. As one of the largest cellular machineries, a vertebrate NPC consists of cytoplasmic filaments, a cytoplasmic ring (CR), an inner ring, a nuclear ring, a nuclear basket, and a luminal ring. Each NPC has eight repeating subunits. Structure determination of NPC is a prerequisite for understanding its functional mechanism. In the past two decades, integrative modeling, which combines x-ray structures of individual nucleoporins and subcomplexes with cryo-electron tomography reconstructions, has played a crucial role in advancing our knowledge about the NPC. The CR has been a major focus of structural investigation. The CR subunit of human NPC was reconstructed by cryo-electron tomography through subtomogram averaging to an overall resolution of ~20 Å, with local resolution up to ~15 Å. Each CR subunit comprises two Y-shaped multicomponent complexes known as the inner and outer Y complexes. Eight inner and eight outer Y complexes assemble in a head-to-tail fashion to form the proximal and distal rings, respectively, constituting the CR scaffold. To achieve higher resolution of the CR, we used single-particle cryo-electron microscopy (cryo-EM) to image the intact NPC from the NE of oocytes. Reconstructions of the core region and the Nup358 region of the CR subunit had been achieved at average resolutions of 5 to 8 Å, allowing identification of secondary structural elements. RATIONALE Packing interactions among the components of the CR subunit were poorly defined by all previous EM maps. Additional components of the CR subunit are strongly suggested by the EM maps of 5- to 8-Å resolution but remain to be identified. Addressing these issues requires improved resolution of the cryo-EM reconstruction. Therefore, we may need to enhance sample preparation, optimize image acquisition, and develop an effective data-processing strategy. RESULTS To reduce conformational heterogeneity of the sample, we spread the opened NE onto the grids with minimal force and used the chemical cross-linker glutaraldehyde to stabilize the NPC. To alleviate orientation bias of the NPC, we tilted sample grids and imaged the sample with higher electron dose at higher angles. We improved the image-processing protocol. With these efforts, the average resolutions for the core and the Nup358 regions have been improved to 3.7 and 4.7 Å, respectively. The highest local resolution of the core region reaches 3.3 Å. In addition, a cryo-EM structure of the N-terminal α-helical domain of Nup358 has been resolved at 3.0-Å resolution. These EM maps allow the identification of five copies of Nup358, two copies of Nup93, two copies of Nup205, and two copies of Y complexes in each CR subunit. Relying on the EM maps and facilitated by AlphaFold prediction, we have generated a final model for the CR of the NPC. Our model of the CR subunit includes 19,037 amino acids in 30 nucleoporins. A previously unknown C-terminal fragment of Nup160 was found to constitute a key part of the vertex, in which the short arm, long arm, and stem of the Y complex meet. The Nup160 C-terminal fragment directly binds the β-propeller proteins Seh1 and Sec13. Two Nup205 molecules, which do not contact each other, bind the inner and outer Y complexes through distinct interfaces. Conformational elasticity of the two Nup205 molecules may underlie their versatility in binding to different nucleoporins in the proximal and distal CR rings. Two Nup93 molecules, each comprising an N-terminal extended helix and an ACE1 domain, bridge the Y complexes and Nup205. Nup93 and Nup205 together play a critical role in mediating the contacts between neighboring CR subunits. Five Nup358 molecules, each in the shape of a shrimp tail and named "the clamp," hold the stems of both Y complexes. The innate conformational elasticity allows each Nup358 clamp to adapt to a distinct local environment for optimal interactions with neighboring nucleoporins. In each CR subunit, the α-helical nucleoporins appear to provide the conformational elasticity; the 12 β-propellers may strengthen the scaffold. CONCLUSION Our EM map-based model of the CR subunit substantially expands the molecular mass over the reported composite models of vertebrate CR subunit. In addition to the Y complexes, five Nup358, two Nup205, and two Nup93 molecules constitute the key components of the CR. The improved EM maps reveal insights into the interfaces among the nucleoporins of the CR. [Figure: see text].
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http://dx.doi.org/10.1126/science.abl8280DOI Listing
June 2022

Genetic Dissection of Three Major Quantitative Trait Loci for Spike Compactness and Length in Bread Wheat ( L.).

Front Plant Sci 2022 23;13:882655. Epub 2022 May 23.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Spike compactness (SC) and length (SL) are the components of spike morphology and are strongly related to grain yield in wheat ( L.). To investigate quantitative trait loci (QTL) associated with SC and SL, a recombinant inbred lines (RIL) population derived from the cross of Bailangmai (BLM, a Tibet landrace) and Chuanyu 20 (CY20, an improved variety) was employed in six environments. Three genomic regions responsible for SC and SL traits were identified on chromosomes 2A and 2D using bulked segregant exome sequencing (BSE-Seq). By constructing genetic maps, six major QTL were repeatedly detected in more than four environments and the best linear unbiased estimation (BLUE) datasets, explaining 7.00-28.56% of the phenotypic variation and the logarithm of the odd (LOD) score varying from 2.50 to 13.22. They were co-located on three loci, designed as , , and , respectively. Based on the flanking markers, their interactions and effects on the corresponding trait and other agronomic traits were also analyzed. Comparison analysis showed that and were possibly two novel loci for SC and SL. and showed pleiotropic effects on plant height and grain morphology, while showed effects on spikelet number per spike (SNS) and grain width (GW). Based on the gene annotation, orthologous search, and spatiotemporal expression patterns of genes, and for , and and for were considered as potential candidate genes, respectively. These results will be useful for fine mapping and developing new varieties with high yield in the future.
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http://dx.doi.org/10.3389/fpls.2022.882655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168683PMC
May 2022

m 6A RNA Methylation Decreases Atherosclerotic Vulnerable Plaque Through Inducing T Cells.

Braz J Cardiovasc Surg 2022 Jun 8. Epub 2022 Jun 8.

Department of Cardiology, Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, People's Republic of China.

Introduction: Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown.

Methods: A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group).

Results: Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P<0.05). Th cells were screened by cell flow. The level of m 6A RNA methylation in the FTO knockdown group was significantly higher than in the vulnerable plaque group and empty load group (P<0.05). The levels of total cholesterol, triglyceride, and low-density lipoprotein C were higher at the 12th week than at the 1st week, but the high-density lipoprotein C level was lower at the 12th week than at the 1st week. At the 12th week, the interleukin-7 level was significantly lower in the adeno-associated virus-9 (AVV9)-FTO short hairpin RNA group than in the control and AVV9-green fluorescent protein groups (P<0.001).

Conclusion: After successfully establishing a vascular parkinsonism rabbit model, m 6A RNA methylation can decrease Th cells and vulnerable atherosclerotic plaques.
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http://dx.doi.org/10.21470/1678-9741-2021-0039DOI Listing
June 2022

Reduction of psychological cravings and anxiety in women compulsorily isolated for detoxification using autonomous sensory meridian response (ASMR).

Brain Behav 2022 07 8;12(7):e2636. Epub 2022 Jun 8.

Department of Psychology, Wenzhou Medical University, Wenzhou, China.

Objective: To explore the effects of the autonomous sensory meridian response (ASMR) on the psychological cravings and anxiety of women compulsorily isolated for detoxification.

Method: Around 122 women were recruited in a female drug detoxification center. Except for the 12-week training of ASMR, the experimental conditions of the experimental group (n = 60) were the same as those of the control group (n = 62). The addiction Stroop task was used to assess the level of psychological cravings and the State-Trait Anxiety Inventory was used to assess the level of anxiety.

Results: After the training, the decrease in state anxiety of the experimental group was larger than that of the control group, and the reaction time of the experimental group in the Stroop was also significantly lower than before the training.

Conclusions: ASMR could thus reduce to a certain extent the state anxiety and attentional bias for drug-related clues under signaling psychological cravings among women compulsorily isolated for detoxification.

Highlights: Intervention effects on psychological cravings and anxiety of women isolated for detoxification Basis for role of ASMR in regulating psychological cravings and anxiety in forced abstainers ASMR intervention reduced forced abstainers' attentional bias to drug-related clues.
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http://dx.doi.org/10.1002/brb3.2636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304838PMC
July 2022

Using large-scale multi-module NRPS to heterologously prepare highly efficient lipopeptide biosurfactants in recombinant Escherichia coli.

Enzyme Microb Technol 2022 Sep 23;159:110068. Epub 2022 May 23.

Department of Emergency, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China. Electronic address:

The apparent solubility and bioavailability of hydrophobic compounds, the main problems in the bioremediation process, can be overcome by bacteria producing biosurfactants. Lipopeptide biosurfactants, as high-efficiency biosurfactants with biological activity, can be synthesized by nonribosomal peptide synthetase (NRPS). In this study, two strains, Paenibacillus lautus A (HC_A) and Lysinibacillus fusiformis B (HC_B), which can efficiently produce lipopeptide biosurfactants, were screened. The NRPS gene sequences of these two strains were obtained by whole-genome sequencing. The module of synthetic surfactant NRPS from HC_A and the module of synthetic surfactants NRPS and sfp from HC_B were successfully cloned into plasmids and then expressed in E. coli (namely, E. coli pHC_A-NRPS and E. coli pHC_B-NRPS-sfp, respectively). Finally, stable lipopeptide biosurfactants could be expressed heterologously. Our research shows that the constructed recombinant bacteria E. coli pHC_A-NRPS and E. coli pHC_B-NRPS-sfp can heterologously express the NRPS module (graphical abstract is shown in Fig 1). Remarkably, the lipopeptide biosurfactants produced by the constructed recombinant bacteria of the NRPS gene have good emulsifying activity on tributyrin. Meanwhile, adding SiO nanoparticles and surface-modified carbon black can synergistically enhance the emulsifying effect, which has not been reported before. This research may deepen the comprehension of microbial strains that produce surfactants to emulsify environmental pollutants and perform bioremediation.
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http://dx.doi.org/10.1016/j.enzmictec.2022.110068DOI Listing
September 2022

COVID-19 and Plasmodium falciparum co-infection in a man returning from Cameroon.

Travel Med Infect Dis 2022 May 31;49:102366. Epub 2022 May 31.

Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases, KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.tmaid.2022.102366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151459PMC
May 2022
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