Publications by authors named "Qiang Zhang"

4,187 Publications

  • Page 1 of 1

Sequentially Regulating the Structural Transformation of Copper Metal-Organic Frameworks (Cu-MOFs) for Controlling Site-Selective Reaction.

ACS Appl Mater Interfaces 2022 Aug 8. Epub 2022 Aug 8.

Center for Advanced Materials Research and Henan Key Laboratory of Functional Salt Materials, Zhongyuan University of Technology, Zhengzhou 450007, China.

Regulating atomically precise sites in catalysts to achieve site-selective reactions is remarkable but challenging. In this work, a convenient and facile solid-gas/liquid reaction strategy was used to construct controllable active sites in metal-organic frameworks (MOFs) to guide an orientation site-selective reaction. A flexible Cu-MOF- with dynamics originating from an anionic and tailorable framework could undergo a reversible structural transformation to engineer a topologically equivalent mixed-valent CuCu-MOF- via a solid-gas/liquid oxidation/reduction process. More importantly, Cu-MOF- and CuCu-MOF- could further execute the solid-gas/liquid reaction under ammonia vapor/solution to generate Cu-MOF-. Furthermore, the transformation from Cu-MOF- to CuCu-MOF- and Cu-MOF- served as controllable catalysts to facilitate site-selective reactions to realize direct C-N bond arylations. The results demonstrated that Cu-MOF- and Cu-MOF- possessed well-defined platforms with uniformly and accurately active sites to attain a "turn-on/off" process via different reaction routes, providing the desired site-selective ring-opening products.
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http://dx.doi.org/10.1021/acsami.2c09290DOI Listing
August 2022

Operando Quantified Lithium Plating Determination Enabled by Dynamic Capacitance Measurement in Working Li-Ion Batteries.

Angew Chem Int Ed Engl 2022 Aug 8. Epub 2022 Aug 8.

Tsinghua University, Department of Chemical Engineering, No.1, Tsinghua Road, 100084, Beijing, CHINA.

The access to full performance of state-of-the-art Li-ion batteries (LIBs) is hindered by the mysterious lithium plating behavior. A rapid quantified lithium plating determination method compatible with actual working conditions is an urgent necessity for safe working LIBs. In this contribution, the relationship between electrical double layer (EDL) capacitance and electrochemical active surface area (ECSA) of graphite anodes during the Li-ion intercalation and Li plating processes is unveiled. We propose an operando lithium plating determination method based on the dynamic capacitance measurement (DCM) test. Reasonable selection of alternating current (AC) frequency protects the anodic responses from the interference of cathodic responses, which allows DCM to be applied in practical LIBs. The onset of lithium plating can be quantitatively traced, demonstrating the promise for real-time operando determination for lithium plating in a working battery.
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http://dx.doi.org/10.1002/anie.202210365DOI Listing
August 2022

Factors Affecting the Perception of Strabismus Among Pediatric Ophthalmologists.

J Pediatr Ophthalmol Strabismus 2022 Aug 8:1-7. Epub 2022 Aug 8.

Purpose: To define potential factors that influence the perceived urgency of strabismus surgery with a specific focus on the contributions of gender, degree of strabismus, and direction of strabismus.

Methods: An electronic survey was sent to members of the American Association for Pediatric Ophthalmology and Strabismus (AAPOS). Respondents provided demographic information and ranked eight photographs of adults digitally altered to create varying degrees of strabismus according to perceived urgency for surgery.

Results: Pediatric ophthalmologists ranked deviations of increasing size with increasing treatment urgency. Men were perceived with higher urgency for treatment compared to women in the smaller angles of strabismus. No consistent preference for type of deviation was found.

Conclusions: The gender of the patient and the amount of misalignment may influence the urgency of surgical management among strabismus surgeons. .
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http://dx.doi.org/10.3928/01913913-20220615-01DOI Listing
August 2022

Excessive mechanical stress induced temporomandibular joint osteoarthritis via osteoclasts-mediated osteogenic differentiation of BMSCs.

J Oral Rehabil 2022 Aug 6. Epub 2022 Aug 6.

Department of Orthodontics II, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, People's Republic of China.

Background: Bone homeostasis is a dynamic process maintained by osteoblasts and osteoclasts, which may be regulated by excessive mechanical stress (EMS).

Objectives: Our study aims to explore the relationship between osteogenic differentiation of BMSCs and EMS-activated osteoclast differentiation of RAW 264.7 cells in order to optimize orthodontic treatment.

Methods: We established the model of EMS in vivo and in vitro. In vivo, HE, Safranin-O staining, micro-CT, and immunofluorescence double-labeling were utilized to assess the changes in condylar, the distributions of osteoblasts, osteoclasts and MAPKs. In vitro, the effects of EMS-activated osteoclast differentiation exerting on osteogenic differentiation of BMSCs were observed by Western Blot, qRT-PCR and Alizarin Red staining. Furthermore, the role of MAPKs in this progress was explored by using inhibitors of MAPKs and co-culture supernatants.

Results: In vivo, EMS led to the degradation of condylar cartilage and destruction of subchondral bone, diagnosed as temporomandibular joint osteoarthritis (TMJ OA). Osteoclasts and osteoblasts were both enriched in subchondral bone, but osteoclast predominated. The expressions of p-JNK, p-ERK1/2, and p-p38 were all activated in vitro and in vivo, which were localized mainly in the Trap+ area in subchondral bone. Interestingly, only the inactivation of p-ERK1/2 in osteoclasts significantly inhibited the osteogenic differentiation of BMSCs in vitro. This revealed that p-ERK1/2 played a key role in the osteoclasts-induced osteogenic differentiation of BMSCs.

Conclusion: Our results proved that EMS led to TMJ OA, in which up-regulated p-ERK1/2 in osteoclasts was mechanosensitive and facilitated the osteogenic differentiation of BMSCs.
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http://dx.doi.org/10.1111/joor.13360DOI Listing
August 2022

Output Enhancement of Triboelectric Nanogenerators Based on Hierarchically Regular Cadmium Coordination Polymers for Photocycloaddition.

Inorg Chem 2022 Aug 5. Epub 2022 Aug 5.

Center for Advanced Materials Research, Henan Key Laboratory of Functional Salt Materials, Zhongyuan University of Technology, Zhengzhou 450007, China.

Exploiting the well-arranged and tunable frameworks of crystalline materials, we herein report coordination polymers (CPs) with modulated hierarchical structures as triboelectric materials to construct and extend the application scope of triboelectric nanogenerators (TENGs). Different lengths and shapes of bridging ligands [4,4'-bpa = 1,2-bis(4-pyridyl)ethane, 4,4'-bpe = 1,2-bis(4-pyridyl)ethene, and 4,4'-bpp = 1,3-di(2-pyridyl)propane for , , and , respectively] were used to construct Cd-CP-based hierarchical frameworks. These compounds were used as triboelectric materials, and their electronic structure contributions were determined by the output of the corresponding TENGs. The results indicated that -TENG with the 4,4'-bpe ligand had the highest output, attributed to the improvement in the electron activity due to the π-conjugation group in the bridging ligand, which was further verified by density functional theory calculations. Furthermore, @PVDF (PVDF = polyvinylidene fluoride) composite films with different concentrations of Cd-CP were prepared. Detailed electrical characterizations revealed that the arrangement of 12% active constituents of Cd-CP- effectively enhanced the output performance of @PVDF-TENG, which could light up an ultraviolet lamp plate to successfully execute the [2 + 2] photocycloaddition.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01810DOI Listing
August 2022

An anti-bacterial and anti-cancer fibrous membrane with multiple therapeutic effects for prevention of pancreatic cancer recurrence.

Biomater Adv 2022 Jun 2;137:212831. Epub 2022 May 2.

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China. Electronic address:

Adjuvant systemic chemotherapy with gemcitabine (GEM) is recognized as the standard of care to improve the prognosis of patients with resected pancreatic cancer (PC); however, it is greatly limited by poor absorption of chemotherapy agents. Moreover, surgical site infection and Gammaproteobacteria-induced GEM resistance further decrease the chemotherapy efficacy and increase the risk of recurrence and even mortality. Here, we develop an implantable anti-bacterial and anti-cancer fibrous membrane (AAFM) to inhibit PC recurrence in a well-coordinated manner. Our AAFM can be readily prepared via simple co-electrospinning of GEM and poly-L-lactic acid (PLLA) and subsequent tannic acid (TA)-mediated in-situ generation of silver nanoparticles (AgNPs). The resultant membrane presents highly porous fibrous morphology and appropriate mechanical performance. Most importantly, we find the surface-deposited TA/AgNP complexes can exert multiple therapeutic effects: (1) they can act as a fence to extend GEM diffusion route, achieving a sustained drug release; (2) they can fight the pathogenic microorganisms in the local microenvironment and prevent infectious complications and alleviate Gammaproteobacteria-induced chemotherapy resistance; (3) they can combat residual cancer cells to synchronously strengthen the effectiveness of GEM-based chemotherapy. Altogether, our AAFM provides a proof-of-concept demonstration of the integrated anti-cancer and anti-bacterial strategy for enhanced therapeutic efficacy and will inspire the design of other high-performance implants for prevention of tumor relapse.
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http://dx.doi.org/10.1016/j.bioadv.2022.212831DOI Listing
June 2022

Codon-optimized FAM132b gene therapy prevents dietary obesity by blockading adrenergic response and insulin action.

Int J Obes (Lond) 2022 Aug 3. Epub 2022 Aug 3.

The Key Laboratory of Adolescent Health Assessment and Exercise Intervention (Ministry of Education), East China Normal University, Shanghai, 200241, China.

Background: FAM132b (myonectin) has been identified as a muscle-derived myokine with exercise and has hormone activity in circulation to regulate iron homeostasis and lipid metabolism via unknown receptors. Here, we aim to explore the potential of adeno-associated virus to deliver FAM132b in vivo to develop a gene therapy against obesity.

Methods: Adeno-associated virus AAV9 were engineered to induce overexpression of FAM132b with two mutations, A136T and P159A. Then, AAV9 was delivered into high-fat diet mice through tail vein, and glucose homeostasis and obesity development of mice were observed. Methods of structural biology were used to predict the action site or receptor of the FAM132b mutant.

Results: Treatment of high-fat diet-fed mice with AAV9 improved glucose intolerance and insulin resistance, and resulted in reductions in body weight, fat depot, and adipocyte size. Codon-optimized FAM132b (coFAM132b) reduced the glycemic response to epinephrine (EPI) in the whole body and increased the lipolytic response to EPI in adipose tissues. However, FAM132b knockdown by shRNA significantly increased the glycemic response to EPI in vivo and reduced adipocyte response to EPI and adipose tissue browning. Structural analysis predicted that the FAM132b mutant with A136T and P159A may form a weak bond with β2 adrenergic receptor (ADRB2) and may have more affinity for insulin and insulin-receptor complexes.

Conclusions: Our study underscores the potential of FAM132b gene therapy with codon optimization to treat obesity by modulating the adrenergic response and insulin action. Both structural biological analysis and in vivo experiments suggest that the adrenergic response and insulin action are most likely blockaded by FAM132b mutants.
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http://dx.doi.org/10.1038/s41366-022-01189-xDOI Listing
August 2022

Steam-assisted crystallization of highly dispersed nanosized hierarchical zeolites from solid raw materials and their catalytic performance in lactide production.

Chem Sci 2022 Jul 21;13(27):8052-8059. Epub 2022 Jun 21.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University 2699 Qianjin Street Changchun 130012 P. R. China

A solvent-free route based on solid raw materials affords higher product yield and lower waste production compared to the traditional hydrothermal synthesis. However, the as-made zeolites usually present blocky aggregation states, limiting their mass transfer and exposure of active sites in catalytic applications. Herein, highly dispersed nanosized hierarchical Beta zeolites with varied Si/Al ratios were prepared steam-assisted crystallization from ball-milled solid raw materials. Thanks to the sufficient mixing of solid raw materials and favorable migration of solid mixture, nanosized Beta zeolites are obtained that are assembled from nanoparticles (∼15 nm) and possess abundant interconnected intraparticle mesopores. The strategy can also be extended to synthesize nanosized hierarchical ZSM-5 zeolites. The as-prepared Beta zeolite (Si/Al = 10) exhibits outstanding catalytic performance in conversion of lactic acid to lactide (as high as 77.5% in yield). This work provides avenues for simple and cost-efficient synthesis of highly dispersed nanosized hierarchical zeolites, promising their important catalytic applications.
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http://dx.doi.org/10.1039/d2sc02823aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278505PMC
July 2022

The Impact of Heat Treatment of Quercetin and Myricetin on their Activities to Alleviate the Acrylamide-Induced Cytotoxicity and Barrier Loss in IEC-6 Cells.

Plant Foods Hum Nutr 2022 Aug 2. Epub 2022 Aug 2.

College of Food Engineering, Harbin University of Commerce, 150028, Harbin, People's Republic of China.

Two flavonols quercetin and myricetin were assessed for their in vitro activities to attenuate the acrylamide-induced cytotoxicity and barrier loss in rat intestinal epithelial (IEC-6) cells and to identify whether heat treatment of the flavonols might cause activity changes. The results showed that the flavonols could alleviate the acrylamide-caused cell injury, resulting in higher cell viability, lower lactate dehydrogenase release, and less formation of reactive oxygen species. Meanwhile, the flavonols could antagonize the acrylamide-induced barrier dysfunction via decreasing the paracellular permeability, increasing the transepithelial resistance of cell monolayer, and enhancing the expression of three tight junction proteins namely occludin, claudin-1, and zonula occludens-1. The flavonols also could down-regulate the expression of JNK/Src proteins and thus cause lower relative protein ratios of p-JNK/JNK and p-Src/Src, resulting in a suppressed JNK/Src activation. Totally, quercetin was more potent than myricetin to exert these assessed activities, while the heated flavonols obtained lower activity than the unheated ones. It is thus concluded that the flavonols had beneficial activities towards the intestinal epithelial cells with acrylamide exposure by alleviating the acrylamide-induced cytotoxicity and barrier disruption, while heat treatment of the flavonols was unfavorable because it led to a reduced flavonol activity to the cells.
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http://dx.doi.org/10.1007/s11130-022-00994-zDOI Listing
August 2022

Circular RNA circTTBK2 facilitates non-small-cell lung cancer malignancy through the miR-873-5p/TEAD1/DERL1 axis.

Epigenomics 2022 Aug 2. Epub 2022 Aug 2.

Department of Respiratory & Critical Care Medicine, The Second Hospital of Jilin University, Changchun, Jilin Province, 130000, P.R. China.

The underlying mechanisms by which circular RNAs (circRNAs) regulate non-small-cell lung cancer (NSCLC) progression remain elusive. This study investigated the role of circRNA circTTBK2 in NSCLC tumorigenesis. Quantitative reverse transcriptase polymerase chain reaction analysis of circTTBK2 in NSCLC tissues and cell lines was performed. Cell proliferation, migration, invasion and tumorigenesis were confirmed and using CCK-8, EdU incorporation, Transwell assays and xenograft technique. The circTTBK2/miR-873-5p/TEAD1/DERL1 axis was verified by RNA immunoprecipitation, chromatin immunoprecipitation and luciferase reporter assays. Overexpressed circTTBK2 in NSCLC tissues indicates poor prognosis of NSCLC patients. circTTBK2 harbors miR-873-5p, and miR-873-5p directly targets TEAD1. TEAD1 transcriptionally activates DERL1. This study revealed a novel machinery of circTTBK2/miR-873-5p/TEAD1/DERL1 for NSCLC tumorigenesis.
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http://dx.doi.org/10.2217/epi-2021-0480DOI Listing
August 2022

Efficacy of Traditional Chinese Medicine Injection in Preventing Oxaliplatin-Induced Peripheral Neurotoxicity: An Analysis of Evidence from 3598 Patients.

Evid Based Complement Alternat Med 2022 22;2022:6875253. Epub 2022 Jul 22.

Department of Oncology, Army Medical Center of PLA, Chongqing 400042, China.

Background: Oxaliplatin is an effective chemotherapeutic agent for the treatment of malignant tumors. However, severe oxaliplatin-induced peripheral neurotoxicity (OIPN) has been well documented. Traditional Chinese medicine injections (TCMIs) have shown significant efficacy in preventing OIPN. However, it is difficult for clinicians to determine the differences in the efficacy of various TCMIs in preventing OIPN. The aim of this study was to compare the efficacy of various TCMIs in preventing OIPN through a network meta-analysis (NMA) to further inform clinical decision-making.

Methods: The Chinese Journal Full Text Database, Chinese Biomedical Literature Database, Wanfang Data Knowledge Service Platform, Chinese Science and Technology Journal Full Text Database, the Cochrane Library, Web of Science, PubMed, and Embase databases were searched for randomized controlled trials (RCTs) of TCMIs for OIPN prevention. The retrieval time was from the establishment of the database to April 12, 2021. NMA was performed using Stata 14.0 software after 2 evaluators independently screened the literature, extracted information, and evaluated the risk of bias of the included studies.

Results: A total of 45 eligible RCTs involving 3598 cancer patients and 13 TCMIs were included. The 13 TCMIs included Xiaoaiping injection (XAPI), compound kushen injection (CKSI), Aidi injection (ADI), Brucea javanica oil emulsion injection (BJOEI), Shenmai injection (SMI), Kangai injection (KAI), injection (AI), elemene emulsion injection (EEI), Shenfu injection (SFI), Shenqi Fuzheng injection (SIFZI), Kanglaite injection (KLEI), Huachansu injection (HCSI), and lentinan injection (LI). NMA results showed that AI was superior to AD and SIFZI was superior to ADI in reducing the incidence of grade I neurotoxicity. SIFZI was superior to EEI and ADI, and BJOEI was superior to chemotherapy alone in reducing the incidence of grade II neurotoxicity. SMI was superior to LI and CKSI in reducing the incidence of grade III neurotoxicity. SIFZI was superior to LI, BJOEI, XAPI, EEI, SMI, chemotherapy alone, HCSI, KLEI, and ADI in reducing the total incidence of grade I-IV neurotoxicity. SFI was superior to ADI. Based on the SUCRA values, AI was the most likely intervention to reduce the incidence of grade I neurotoxicity, SIFZI was the most likely intervention to reduce the total incidence of grade II and I-IV neurotoxicity, and SMI was the most likely intervention to reduce the incidence of grade III and IV neurotoxicity.

Conclusion: TCMIs can prevent OIPN to some extent, among which SIFZI, SMI, and AI may be the most promising TCMIs. However, given the limitations of current studies, more well-designed, high-quality clinical trials will be needed in the future to validate the benefits of TCMIs.
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http://dx.doi.org/10.1155/2022/6875253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337932PMC
July 2022

Changes in Gallbladder Contractile Function and its Influencing Factors After Minimally Invasive Gallbladder-Preserving Surgery for Cholecystitis With Incarcerated Gallstones.

Front Surg 2022 14;9:926141. Epub 2022 Jul 14.

Department of Hepatobiliary Surgery, Aerospace Center Hospital, Beijing, China.

Background: Cholecystitis with incarcerated gallstones (CIG) is a type of acute abdomen in the field of hepatobiliary surgery. Whether gallbladder-preserving surgery (GPS) can be performed to treat it, however, depends on the improvement of gallbladder contractile function. The present study aimed to investigate the changes in gallbladder contractile function and its influencing factors after minimally invasive GPS for CIG.

Methods: A total of 95 patients with CIG treated in the Aerospace Center Hospital between May 2017 and May 2019 were enrolled as the study subjects. All patients received minimally invasive GPS. The patients' operation-related conditions (including stone removal success rate, duration of surgery, intraoperative blood loss, etc.), changes in gallbladder contractile function, and influencing factors of GPS were analyzed.

Results: Among the 95 patients included in the study, the success rate of stone removal was 100%, the duration of surgery was 76.0 ± 26.5 min, and the intraoperative blood loss was 10.17 ± 4.43 ml. The rate of good gallbladder contractile function at one and two years after surgery was significantly higher than before surgery ( < 0.05). Age, duration of surgery, stone recurrence, and diabetes were the independent risk factors for postoperative gallbladder contractile function ( < 0.05).

Conclusion: Minimally invasive GPS for patients with CIG has a good curative effect. The changes in gallbladder contractile function after the surgery are influenced by many factors.
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http://dx.doi.org/10.3389/fsurg.2022.926141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329626PMC
July 2022

Jujuboside A inhibits oxidative stress damage and enhances immunomodulatory capacity of human umbilical cord mesenchymal stem cells through up-regulating IDO expression.

Chin J Nat Med 2022 Jul;20(7):494-505

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. Electronic address:

Impaired immunomodulatory capacity and oxidative stress are the key factors limiting the effectiveness of mesenchymal stem cell transplantation therapy. The present study was aimed to investigate the effects of jujuboside A (JuA) on the protective effect and immunomodulatory capacity of human umbilical cord mesenchymal stem cells (hUC-MSCs). Hydrogen peroxide was used to establish an oxidative damage model of hUC-MSCs, while PBMCs isolated from rats were used to evaluate the effect of JuA pre-treatment on the immunomodulatory capacity of hUC-MSCs. Furthermore, Hoechst 33258 staining, lactate dehydrogenase test, measurement of malondialdehyde, Western blot, high-performance liquid chromatography; and flow cytometry were performed. Our results indicated that JuA (25 μmol·L) promoted the proliferation of hUC-MSCs, but did not affect the differentiating capability of these cells. JuA pre-treatment inhibited apoptosis, prevented oxidative damage, and up-regulated the protein expression of nuclear factor-erythroid factor 2-related factor 2 and heme oxygenase 1 in hUC-MSCs in which oxidative stress was induced with HO. In addition, JuA pre-treatment enhanced the inhibitory effect of hUC-MSCs against abnormally activated PBMCs, which was related to stimulation of the expression and activity of indoleamine 2,3-dioxygenase. In conclusion, our results demonstrate that JuA pre-treatment can enhance the survival and immunomodulatory ability through pathways related to oxidative stress, providing a new option for the improvement of hUC-MSCs in the clinical setting.
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http://dx.doi.org/10.1016/S1875-5364(22)60176-6DOI Listing
July 2022

STTG-net: a Spatio-temporal network for human motion prediction based on transformer and graph convolution network.

Vis Comput Ind Biomed Art 2022 Jul 29;5(1):19. Epub 2022 Jul 29.

School of Computer Science and Technology, Dalian University of Technology, Dalian, 116024, China.

In recent years, human motion prediction has become an active research topic in computer vision. However, owing to the complexity and stochastic nature of human motion, it remains a challenging problem. In previous works, human motion prediction has always been treated as a typical inter-sequence problem, and most works have aimed to capture the temporal dependence between successive frames. However, although these approaches focused on the effects of the temporal dimension, they rarely considered the correlation between different joints in space. Thus, the spatio-temporal coupling of human joints is considered, to propose a novel spatio-temporal network based on a transformer and a gragh convolutional network (GCN) (STTG-Net). The temporal transformer is used to capture the global temporal dependencies, and the spatial GCN module is used to establish local spatial correlations between the joints for each frame. To overcome the problems of error accumulation and discontinuity in the motion prediction, a revision method based on fusion strategy is also proposed, in which the current prediction frame is fused with the previous frame. The experimental results show that the proposed prediction method has less prediction error and the prediction motion is smoother than previous prediction methods. The effectiveness of the proposed method is also demonstrated comparing it with the state-of-the-art method on the Human3.6 M dataset.
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http://dx.doi.org/10.1186/s42492-022-00112-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338210PMC
July 2022

Hidden Local Symmetry Breaking in a Kagome-Lattice Magnetic Weyl Semimetal.

J Am Chem Soc 2022 Jul 28. Epub 2022 Jul 28.

Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.

Exploring the relationship between intriguing physical properties and structural complexity is a central topic in studying modern functional materials. CoSnS, a newly discovered kagome-lattice magnetic Weyl semimetal, has triggered intense interest owing to the intimate coupling between topological semimetallic states and peculiar magnetic properties. However, the origins of the magnetic phase separation and spin glass state below in this ordered compound are two unresolved yet important puzzles in understanding its magnetism. Here, we report the discovery of local symmetry breaking surprisingly co-emerges with the onset of ferromagnetic order in CoSnS, by a combined use of neutron total scattering and half-polarized neutron diffraction. An anisotropic distortion of the cobalt kagome lattice at the atomic/nano level is also found, with distinct distortion directions among the two Co1 and four Co2 atoms. The mismatch of local and average symmetries occurs below , indicating that CoSnS evolves to an intrinsically lattice disordered system when the ferromagnetic order is established. The local symmetry breaking with intrinsic lattice disorder provides new understanding of the puzzling magnetic properties. Our density functional theory (DFT) calculation indicates that the local symmetry breaking is expected to reorient local ferromagnetic moments, unveiling the existence of the ferromagnetic instability associated with the lattice instability. Furthermore, DFT calculation unveils that the local symmetry breaking could affect the Weyl property by breaking the mirror plane. Our findings highlight the fundamentally important role that the local symmetry breaking plays in advancing our understanding on the magnetic and topological properties in CoSnS, which may draw attention to explore the overlooked local symmetry breaking in CoSnS, its derivatives and more broadly in other topological Dirac/Weyl semimetals and kagome-lattice magnets.
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http://dx.doi.org/10.1021/jacs.2c05665DOI Listing
July 2022

Proteomic and Phosphoproteomic Landscapes of Acute Myeloid Leukemia.

Blood 2022 Jul 27. Epub 2022 Jul 27.

Washington University School of Medicine, St Louis, Missouri, United States.

We have developed a deep-scale proteome and phosphoproteome database from 44 representative Acute Myeloid Leukemia (AML) patients from the LAML TCGA dataset, and 6 healthy bone marrow derived controls. After confirming data quality, we orthogonally validated several previously undescribed features of AML revealed by the proteomic data. We identified examples of post-transcriptionally regulated proteins both globally (i.e. in all AML samples), and also, in patients with recurrent AML driver mutations. For example, samples with IDH1/2 mutations displayed elevated levels of the 2‑oxoglutarate-dependent histone demethylases KDM4A/B/C, despite no changes in mRNA levels for these genes; we confirmed this finding in vitro. In samples with NPMc mutations, we identified several nuclear importins with post‑transcriptionally increased protein abundance, and showed that they interact with NPMc, but not wildtype NPM1. We identified two cell surface proteins (CD180 and MRC1/CD206) expressed on AML blasts of many patients (but not healthy CD34+ stem/progenitor cells) that could represent novel targets for immunologic therapies, and confirmed these targets via flow cytometry. Finally, we detected nearly 30,000 phosphosites in these samples; globally, AML samples were associated with the abnormal phosphorylation of specific residues in PTPN11, STAT3, AKT1 and PRKCD. FLT3‑TKD samples were associated with increased phosphorylation of activating tyrosines on the cytoplasmic Src-family tyrosine kinases FGR and HCK, and related signaling proteins. PML-RARA-initiated AML samples displayed a unique phosphorylation signature, and TP53-mutant samples showed abundant phosphorylation of serine-183 on TP53 itself. This publicly available database will serve as a foundation for further investigations of protein dysregulation in AML pathogenesis.
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http://dx.doi.org/10.1182/blood.2022016033DOI Listing
July 2022

Growth, Yield and Photosynthetic Performance of Winter Wheat as Affected by Co-Application of Nitrogen Fertilizer and Organic Manures.

Life (Basel) 2022 Jul 6;12(7). Epub 2022 Jul 6.

Agricultural Institute, Centre for Agricultural Research, ELKH, 2462 Martonvásár, Hungary.

The application of organic manures was found to be beneficial, however, the integrated use of organic manures with chemical nitrogen fertilizers has proven more sustainable in increasing the photosynthetic attributes and grain yield of the winter-wheat crop. A multi-factor split-plot design was adopted, nitrogen and manure fertilizer treatments were set in the sub-plots, including nitrogen-gradient treatment of T1:0 kg N ha, T2:100 kg N ha, T3:200 kg N ha, and T4:300 kg N ha (pure nitrogen -fertilizer application) The 25% reduction in nitrogen combined with the manure-fertilizer application includes T5:75 kg N ha nitrogen and 25 kg N ha manure, T6:150 kg N ha nitrogen and 50 kg N ha manure, and T7:225 kg N ha nitrogen and 75 kg N ha manure. The maximum results of the total chlorophyll content and photosynthetic rate were 5.73 mg/g FW and 68.13 m mol m s, observed under T4 in Zhongmai 175, as compared to Jindong 22 at the heading stage. However, the maximum results of intercellular CO concentration were 1998.47 μmol mol, observed under T3 in Jindong 22, as compared to Zhongmai 175 at the tillering stage. The maximum results of LAI were 5.35 (cm), observed under T7 in Jindong 22, as compared to Zhongmai 175 at the booting stage. However, the maximum results of Tr and Gs were 6.31 mmol HO m s and 0.90 HO mol m s, respectively, observed under T7 in Zhongmai 175 as compared to Jindong 22 at the flowering stage. The results revealed that grain yield 8696.93 kg ha, grains spike 51.33 (g), and 1000-grain weight 39.27 (g) were significantly higher, under T3 in Zhongmai 175, as compared to Jindong 22. Moreover, the spike number plot of 656.67 m was significantly higher in Jindong 22, as compared to Zhongmai 175. It was concluded from the study that the combined application of nitrogen and manure fertilizers in winter wheat is significant for enhancing seed at the jointing and flowering stages. For increased grain yield and higher economic return, Zhongmai 175 outperformed the other cultivars examined. This research brings awareness toward the nitrogen-fertilizer-management approach established for farmers' practice, which might be observed as an instruction to increase agricultural management for the winter-wheat-growth season.
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http://dx.doi.org/10.3390/life12071000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319553PMC
July 2022

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.

Brain Sci 2022 Jul 15;12(7). Epub 2022 Jul 15.

Department of Emergency Medicine, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China.

Predicting neurological outcomes after cardiac arrest remains a major issue. This study aimed to identify novel biomarkers capable of predicting neurological prognosis after cardiac arrest. Expression profiles of GSE29540 and GSE92696 were downloaded from the Gene Expression Omnibus (GEO) database to obtain differentially expressed genes (DEGs) between high and low brain performance category (CPC) scoring subgroups. Weighted gene co-expression network analysis (WGCNA) was used to screen key gene modules and crossover genes in these datasets. The protein-protein interaction (PPI) network of crossover genes was constructed from the STRING database. Based on the PPI network, the most important hub genes were identified by the cytoHubba plugin of Cytoscape software. Eight hub genes (RPL27, EEF1B2, PFDN5, RBX1, PSMD14, HINT1, SNRPD2, and RPL26) were finally screened and validated, which were downregulated in the group with poor neurological prognosis. In addition, GSEA identified critical pathways associated with these genes. Finally, a Pearson correlation analysis showed that the mRNA expression of hub genes EEF1B2, PSMD14, RPFDN5, RBX1, and SNRPD2 were significantly and positively correlated with NDS scores in rats. Our work could provide comprehensive insights into understanding pathogenesis and potential new biomarkers for predicting neurological outcomes after cardiac arrest.
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http://dx.doi.org/10.3390/brainsci12070928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316619PMC
July 2022

Gender differences affect the location of the patellar tendon attachment site for tibial rotational alignment in total knee arthroplasty.

J Orthop Surg Res 2022 Jul 26;17(1):362. Epub 2022 Jul 26.

Department of Orthopedic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 Huaihai Road, Xuzhou, 221002, Jiangsu, China.

Purpose: This study was carried out to investigate the accuracy of referring different locations of the patellar tendon attachment site and the geometrical center of the osteotomy surface for tibial rotational alignment and observe the influences of gender differences on the results.

Methods: Computed tomography scans of 135 osteoarthritis patients (82 females and 53 males) with varus deformity was obtained to reconstruct three-dimensional (3D) models preoperatively. The medial boundary, medial one-sixth, and medial one-third of the patellar tendon attachment site were marked on the tibia. These points were projected on the tibial osteotomy plane and connected to the geometrical center (GC) of the osteotomy plane or the middle of the posterior cruciate ligament (PCL) to construct six tibial rotational axes (Akagi line, MBPT, MSPT1, MSPT2, MTPT1 and MTPT2). The mismatch angle between the vertical line of the SEA projected on the proximal tibial osteotomy surface and six different reference axes was measured. In additional, the effect of gender differences on rotational alignment for tibial component were assessed.

Results: Relative to the SEA, rotational mismatch angles were - 1.8° ± 5.1° (Akagi line), - 2.5° ± 5.3° (MBPT), 2.8° ± 5.3° (MSPT1), 4.5° ± 5.4° (MSPT2), 7.3° ± 5.4° (MTPT1), and 11.6° ± 5.8° (MTPT2) for different tibial rotational axes in all patients. All measurements differed significantly between the male and female. The tibial rotational axes with the least mean absolute deviation for the female or male were Akagi line or MSPT, respectively. There was no significant difference in whether the GC of the osteotomy surface or the midpoint of PCL termination was chosen as the posterior anatomical landmark when the medial boundary or medial one-sixth point of the patellar tendon attachment site was selected as the anterior anatomical landmark.

Conclusion: When referring patellar tendon attachment site as anterior anatomical landmarks for tibial rotational alignment, the influence of gender difference on the accuracy needs to be taken into account. The geometric center of the tibial osteotomy plane can be used as a substitute for the middle of the PCL termination when reference the medial boundary or medial one-sixth of the patellar tendon attachment site.
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http://dx.doi.org/10.1186/s13018-022-03248-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327362PMC
July 2022

Association Between ABO Blood Group and Venous Thromboembolism Risk in Patients With Peripherally Inserted Central Catheters: A Meta-analysis and Systematic Review.

Front Oncol 2022 8;12:906427. Epub 2022 Jul 8.

Department of Oncology, Army Medical Center of PLA, Chongqing, China.

Background: Previous studies have evaluated the association between ABO blood group and venous thromboembolism (VTE) risk in patients with peripherally inserted central catheters (PICCs). However, it remains unclear whether ABO blood groups are associated with PICC-associated VTE risk. Therefore, we conducted a meta-analysis of related studies to elucidate the potential role of ABO blood group as a risk factor for PICC-associated VTE.

Methods: All detectable case-control and cohort studies comparing the role of ABO blood group as a risk factor for PICC-associated VTE were collected for this analysis by searching PubMed, Embase, CNKI, Web of Science, and Wanfang. We conducted a meta-analysis of the eligible studies and computed the summary risk estimates with random or fixed effects models.

Results: A total of four studies involving 7,804 patients were included. Meta-analysis of the studies showed that the risk of PICC-associated VTE was significantly higher in blood types A [odds ratio (OR)=1.54, 95% CI=1.17-2.03), p=0.002], B (OR=2.35, 95% CI=1.71-3.23, p<0.0001), and AB (OR=2.55, 95% CI=1.68-3.88, p<0.0001) and lower in blood types O (OR=0.58, 95% CI=0.45-0.74, p<0.0001). Subgroup analysis based on ethnicity demonstrated that blood type O may be a genetic protective factor for PICC-associated VTE in Asians. Among Caucasians, individuals with blood types B and AB have a higher risk of PICC-associated VTE. Blood types A, B, and AB are risk factors for PICC-associated VTE in Asians.

Conclusions: Blood type O is associated with a decreased risk of PICC-associated VTE, especially in Asian populations. Moreover, blood types A, B, and AB are risk factors for PICC-associated VTE.
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http://dx.doi.org/10.3389/fonc.2022.906427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304918PMC
July 2022

Seminal plasma extracellular vesicles tRF-Val-AAC-010 can serve as a predictive factor of successful microdissection testicular sperm extraction in patients with non-obstructive azoospermia.

Reprod Biol Endocrinol 2022 Jul 22;20(1):106. Epub 2022 Jul 22.

Xuzhou Engineering Research Center of Medical Genetics and Transformation, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.

Background: There is a lack of biomarkers for distinguishing non-obstructive azoospermia (NOA) patients with successful sperm retrieval (Sp+) from those with failed sperm retrieval (Sp-). This study aimed to determine the potential of extracellular vesicles tRNA-derived small RNA (tsRNA) as a novel non-invasive biomarker for successful sperm retrieval by microdissection testicular sperm extraction (mTESE).

Methods: The study included 18 patients with NOA with successful sperm retrieval (Sp+) and 23 patients with NOA with failed sperm retrieval (Sp-), 15 obstructive azoospermia (OA) patients, 5 idiopathic oligospermia (IO) patients, and 12 healthy people. Seminal plasma extracellular vesicles tsRNA levels were used in a two-stage case-control study (screened by tsRNA sequencing on Illumina NextSeq instrument and validated by qRT-PCR). The bioinformatic analysis was performed to determine the role of tsRNA in the pathogenesis of non-obstructive azoospermia.

Results: Two tsRNAs (tRF-Val-AAC-010: AUC = 0.96, specificity = 80%, sensitivity = 95%; tRF-Pro-AGG-003: AUC = 0.96, specificity = 87%, sensitivity = 95%) were found to have high predictive accuracy for distinguishing the origin of azoospermia. In addition, the extracellular vesicles tRF-Val-AAC-010 resulted in high predictive ability (AUC = 0.89, sensitivity = 72%, specificity = 91%, P < 0.0001) in predicting the presence of sperm in non-obstructive azoospermia undergoing mTESE. Finally, bioinformatic analysis revealed that tRF-Val-AAC-010 were involved in spermatogenesis.

Conclusions: This study identified that the extracellular vesicles tRF-Val-AAC-010 and tRF-Pro-AGG-003 are biomarkers for the diagnosis of non-obstructive azoospermia, and that tRF-Val-AAC-010 as a potential non-invasive biomarker for predicting the presence of sperm in non-obstructive azoospermia testicular tissue.
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http://dx.doi.org/10.1186/s12958-022-00978-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308200PMC
July 2022

Gastric bypass was performed with a novel through-the-scope twin clip under endoscopy.

Endoscopy 2022 Jul 21. Epub 2022 Jul 21.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

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http://dx.doi.org/10.1055/a-1866-3582DOI Listing
July 2022

A Survival Status Classification Model for Osteosarcoma Patients Based on E-CNN-SVM and Multisource Data Fusion.

Comput Intell Neurosci 2022 9;2022:9464182. Epub 2022 Jul 9.

School of Artificial Intelligence and Computer Science, Jiangnan University, Wuxi, Jiangsu 214122, China.

Traditional algorithms have the following drawbacks: (1) they only focus on a certain aspect of genetic data or local feature data of osteosarcoma patients, and the extracted feature information is not considered as a whole; (2) they do not equalize the sample data between categories; (3) the generalization ability of the model is weak, and it is difficult to perform the task of classifying the survival status of osteosarcoma patients better. In this context, this paper designs a survival status prediction model for osteosarcoma patients based on E-CNN-SVM and multisource data fusion, taking into full consideration the characteristics of the small number of samples, high dimensionality, and interclass imbalance of osteosarcoma patients' genetic data. The model fuses four gene sequencing data highly correlated with bone tumors using the random forest algorithm in a dimensionality reduction and then equalizes the data using a hybrid sampling method combining the SMOTE algorithm and the TomekLink algorithm; secondly, the CNN model with the incentive module is used to further extract features from the data for more accurate extraction of characteristic information; finally, the data are passed to the SVM model to further improve the stability and classification performance of the model. The model has been demonstrated to be more effective in improving the accuracy of the classification of patients with osteosarcoma.
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http://dx.doi.org/10.1155/2022/9464182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288314PMC
July 2022

Identification by RNA-Seq of let-7 clusters as prenatal biomarkers for nonsyndromic cleft lip with palate.

Ann N Y Acad Sci 2022 Jul 19. Epub 2022 Jul 19.

Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, PR China.

Nonsyndromic cleft lip with palate (nsCLP) is a common congenital malformation; however, early prenatal diagnosis is challenging and pathogenesis remains unclear. The purpose of this study was to determine the diagnostic potential of miRNAs in plasma-derived exosomes and whole plasma of pregnant women to identify nsCLP and an underlying mechanism. Combined RNA sequencing analysis was performed on samples from plasma exosomes and whole plasma of pregnant women carrying normal fetuses or fetuses with nsCLP in an ongoing birth cohort, in addition to lip samples from nsCLP fetuses and healthy controls. Eight let-7 cluster miRNAs (hsa-let-7a-3p, hsa-let-7a-5p, hsa-let-7c-5p, hsa-let-7d-3p, hsa-let-7d-5p, hsa-let-7e-5p, hsa-let-7f-5p, and hsa-miR-98-5p) in plasma exosomes from pregnant women provided higher sensitivity/specificity for diagnosing fetal nsCLP than those in plasma. Area under the receiver operating characteristic curve value of the eight miRNAs from plasma exosomes was 0.992. Among them, hsa-let-7a-3p showed better diagnostic capability and was downregulated in nsCLP fetal lip tissues. Upstream and downstream target genes of hsa-let-7a-3p were screened and confirmed. Our work highlights the potential clinical application value of let-7 clusters in predicting nsCLP and associates as a new regulatory axis (EN2-LIN28A-hsa-let-7a-3p-HHIP-GLI2) with human nsCLP pathogenesis.
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http://dx.doi.org/10.1111/nyas.14868DOI Listing
July 2022

A Pilot Randomized Clinical Trial of a Goals-of-Care Decision Aid for Surrogates of Severe Acute Brain Injury Patients.

Neurology 2022 Jul 19. Epub 2022 Jul 19.

Division of Neurocritical Care and Emergency Neurology, Department of Neurology, Yale School of Medicine, New Haven, CT.

Background And Objectives: Breakdowns in clinician-family communication in neurologic intensive care units (neuroICUs) are common, particularly for goals-of-care decisions to continue or withdraw life-sustaining treatments while considering long-term prognoses. Shared decision-making interventions (decision aids [DAs]) may prevent this problem and increase patient-centered care, yet none are currently available. We assessed the feasibility, acceptability, and perceived usefulness of a DA for goals-of-care communication with surrogate decision-makers for critically ill severe acute brain injury (SABI) patients after hemispheric acute ischemic stroke, intracerebral hemorrhage, or traumatic brain injury.

Methods: We conducted a parallel-arm, unblinded, patient-level randomized, controlled pilot trial at two tertiary-care U.S. neuroICUs and randomized surrogate participants 1:1 to a tailored paper-based DA provided to surrogates prior to clinician-family goals-of-care meetings or usual care (no intervention prior to clinician-family meetings). The primary outcomes were feasibility of deploying the DA (recruitment, participation, retention), acceptability, and perceived usefulness of the DA among surrogates. Exploratory outcomes included outcome of surrogate goals-of-care decision, code-status changes during admission, patients' 3-month functional outcome, and surrogates' 3-month validated psychological outcomes.

Results: We approached 83 surrogates of 58 patients and enrolled 66 surrogates of 41 patients (80% consent rate). Of 66 surrogates, 45 remained in the study at 3 months (68% retention). Of the 33 surrogates randomized to intervention, 27 were able to receive the DA, and 25 subsequently read the DA (93% participation). 82% rated the DA's acceptability as good or excellent (median Acceptability score 2 [IQR 2;3]); 96% found it useful for goals-of-care decision-making. In the DA group, there was a trend towards fewer comfort-care decisions (27% vs. 56%, p=0.1) and fewer code-status changes (no change, 73% vs. 44%, p=0.02). At 3 months, fewer patients in the DA group had died (33% vs. 69%, p=0.05; median GOS 3 vs.1, p=0.05). Regardless of intervention, 3-month psychological outcomes were significantly worse among surrogates who had chosen continuation-of-care.

Discussion: A goals-of-care DA to support ICU shared decision-making for patients with SABI is feasible to deploy and well-perceived by surrogates. A larger trial is feasible to conduct, although surrogates who select continuation-of-care deserve additional psychosocial support.

Clinical Trials Registration: Clinicaltrials.gov NCT03833375 CLASSIFICATION OF EVIDENCE: This study provides Class IV evidence that the use of a DA explaining the goals-of-care decision and the treatment options is acceptable and useful to surrogates of incapacitated critically ill patients with ischemic stroke, intracerebral hemorrhage, or traumatic brain injury.
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http://dx.doi.org/10.1212/WNL.0000000000200937DOI Listing
July 2022

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16 levels.

Nat Cell Biol 2022 Aug 18;24(8):1202-1210. Epub 2022 Jul 18.

Immunology, Microenvironment and Metastasis Program, The Wistar Institute, Philadelphia, PA, USA.

Cellular senescence plays a causal role in ageing and, in mice, depletion of p16-expressing senescent cells delays ageing-associated disorders. Adenosine deaminases acting on RNA (ADARs) are RNA-editing enzymes that are also implicated as important regulators of human ageing, and ADAR inactivation causes age-associated pathologies such as neurodegeneration in model organisms. However, the role, if any, of ADARs in cellular senescence is unknown. Here we show that ADAR1 is post-transcriptionally downregulated by autophagic degradation to promote senescence through p16 upregulation. The ADAR1 downregulation is sufficient to drive senescence in both in vitro and in vivo models. Senescence induced by ADAR1 downregulation is p16-dependent and independent of its RNA-editing function. Mechanistically, ADAR1 promotes SIRT1 expression by affecting its RNA stability through HuR, an RNA-binding protein that increases the half-life and steady-state levels of its target mRNAs. SIRT1 in turn antagonizes translation of mRNA encoding p16. Hence, downregulation of ADAR1 and SIRT1 mediates p16 upregulation by enhancing its mRNA translation. Finally, Adar1 is downregulated during ageing of mouse tissues such as brain, ovary and intestine, and Adar1 expression correlates with Sirt1 expression in these tissues in mice. Together, our study reveals an RNA-editing-independent role for ADAR1 in the regulation of senescence by post-transcriptionally controlling p16 expression.
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http://dx.doi.org/10.1038/s41556-022-00959-zDOI Listing
August 2022

[Granulation of Yangxue Qingnao Granules in fluidized bed based on near-infrared spectroscopy].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(14):3806-3815

State Key Laboratory of Innovative Key Technologies of Traditional Chinese Medicine Tianjin 300410, China.

To realize the real-time monitoring of the production process of Yangxue Qingnao Granules and improve the inter-batch consistency of granule quality in the granulation process, this study established a near-infrared quantitative prediction model of moisture, particle size, bulk density, and angle of repose in the fluidized bed granulation process of Yangxue Qingnao Granules based on near-infrared spectroscopy(NIRS). The near-infrared spectra were collected from 355 samples in 12 batches in the granulation process by integrating the sphere detection module of the near-infrared spectrometer. In combination with the pretreatment methods such as the first derivative, multiplicative scatter correction(MSC), and standard normal variate(SNV), the model was established by partial least squares(PLS) regression. The root mean square error of prediction(RMSEP) of moisture was 0.347 and R_P~2 was 0.935. The RMSEP of the D_(50) particle size model was 38.4 and R_P~2 was 0.980. The RMSEPs of bulk density and angle of repose were 0.018 8 and 0.879, with R_P~2 of 0.085 9 and 0.958. The results showed that the prediction of the PLS quantitative model combined with NIRS was accurate, and this model can be applied to the monitoring of key quality attributes in the fluidized bed granulation of Chinese medicinal granules in the production scale.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211227.301DOI Listing
July 2022

WAL3 encoding a PLS-type PPR protein regulates chloroplast development in rice.

Plant Sci 2022 Jul 16;323:111382. Epub 2022 Jul 16.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. Electronic address:

Chloroplast development is a complex process that is critical for the growth and development of plants. Pentapeptide repeat (PPR) proteins contain large members but only few of them have been characterized in rice. In this study, we identified a new PLS-type protein, WAL3 (Whole Albino Leaf on Chromosome 3), playing important roles in plant chloroplast development. Knockout of WAL3 gene in Nipponbare variety caused abnormal chloroplast development and showed an albino lethal phenotype. Expression analysis showed that WAL3 gene was constitutively expressed with the highest expression in leaves. The WAL3 protein localized in chloroplasts and affected the splicing of multiple group II introns. Transcriptome sequencing showed that WAL3 involved in multiple metabolic pathways including the chlorophyll synthesis and photosynthetic related metabolic pathways. The decreased abundance of photosynthesis-related proteins in wal3 mutants indicated WAL3 influence photosynthesis. In summary, our study revealed that WAL3 is essential for chloroplast development in rice.
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http://dx.doi.org/10.1016/j.plantsci.2022.111382DOI Listing
July 2022

Yifei Sanjie Formula Treats Chronic Obstructive Pulmonary Disease by Remodeling Pulmonary Microbiota.

Front Med (Lausanne) 2022 29;9:927607. Epub 2022 Jun 29.

Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Kunming, China.

Chronic obstructive pulmonary disease (COPD) is one of the most common pulmonary diseases. Evidence suggests that dysbiosis of pulmonary microbiota leads to the COPD pathological process. Yifei Sanjie Formula (YS) is widely used to treat diseases in respiratory systems, yet little is known about its mechanisms. In the present study, we first established the fingerprint of YS as the background for UHPLC-QTOF-MS. Components were detected, including alkaloids, amino acid derivatives, phenylpropanoids, flavonoids, terpenoids, organic acids, phenols, and the like. The therapeutic effect of YS on COPD was evaluated, and the pulmonary function and ventilatory dysfunction (EF50, TV, and MV) were improved after the administration of YS. Further, the influx of lymphocytes was inhibited in pulmonary parenchyma, accompanied by down-regulation of inflammation cytokines the NLRP3/caspase-1/IL-1β signaling pathway. The severity of pulmonary pathological damage was reversed. Disturbed pulmonary microbiota was discovered to involve an increased relative abundance of and and a decreased relative abundance of and in COPD animals. However, the subversive effect was shown. The abundance and diversity of pulmonary microflora were remodeled, especially increasing beneficial genua and , as well as downregulating pathogenic genua and in the YS group. Environmental factor correlation analysis showed that growing pulmonary microbiota was positively correlated with the inflammatory factor, referring to and , as well as negatively correlated with the inflammatory factor, referring to and . These results suggest that the effects of YS involved remodeling lung microbes and anti-inflammatory signal pathways, revealing that intervention microbiota and an anti-inflammatory may be a potential therapeutic strategy for COPD.
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http://dx.doi.org/10.3389/fmed.2022.927607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277004PMC
June 2022

Ferroptosis and Autophagy-Related Genes in the Pathogenesis of Ischemic Cardiomyopathy.

Front Cardiovasc Med 2022 30;9:906753. Epub 2022 Jun 30.

School of Medicine, Nankai University, Tianjin, China.

Background: Obesity plays an important role in type 2 diabetes mellitus (T2DM) and myocardial infarction (MI). Ferroptosis and ferritinophagy are related to metabolic pathways, such as fatty acid metabolism and mitochondrial respiration. We aimed to investigate the ferroptosis- and autophagy-related differentially expressed genes (DEGs) that might be potential targets for MI progression.

Methods: GSE116250 was analyzed to obtain DEGs. A Venn diagram was used to obtain the overlapping ferroptosis- and autophagy-related DEGs. The enrichment pathway analysis was performed and the hub genes were obtained. Pivotal miRNAs, transcription factors, and drugs with the hub genes interactions were also predicted. The MI mice model was constructed, and qPCR analysis and single-cell sequencing were used to validate the hub genes.

Results: Utilizing the limma package and the Venn diagram, 26 ferroptosis-related and 29 autophagy-related DEGs were obtained. The list of ferroptosis-related DEGs was analyzed, which were involved in the cellular response to a toxic substance, cellular oxidant detoxification, and the IL-17 signaling pathway. The list of autophagy-related DEGs was involved in the regulation of autophagy, the regulation of JAK-STAT signaling pathway, and the regulation of MAPK cascade. In the protein-protein interaction network, the hub DEGs, such as IL-6, PTGS2, JUN, NQO1, NOS3, LEPR, NAMPT, CDKN2A, CDKN1A, and Snai1, were obtained. After validation using qPCR analysis in the MI mice model and single-cell sequencing, the 10 hub genes can be the potential targets for MI deterioration.

Conclusion: The screened hub genes, IL-6, PTGS2, JUN, NQO1, NOS3, LEPR, NAMPT, CDKN2A, CDKN1A, and Snai1, may be therapeutic targets for patients with MI and may prevent adverse cardiovascular events.
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http://dx.doi.org/10.3389/fcvm.2022.906753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279674PMC
June 2022
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