Publications by authors named "Qiang Yang"

440 Publications

Enhancing Ferromagnetism and Tuning Electronic Properties of CrI Monolayers by Adsorption of Transition-Metal Atoms.

ACS Appl Mater Interfaces 2021 Apr 27. Epub 2021 Apr 27.

SEU-FEI Nano-Pico Center, Key Laboratory of MEMS of Ministry of Education, Collaborative Innovation Center for Micro/Nano Fabrication, Device and System, Southeast University, Nanjing 210096, China.

Among first experimentally discovered two-dimensional (2D) ferromagnetic materials, chromium triiodide (CrI) monolayers have attracted particular attention due to their potential applications in electronics and spintronics. However, the Curie temperature of the CrI monolayer is below room temperature, which greatly limits practical development of the devices. Herein, using density functional theory calculation, we explore how the electronic and magnetic properties of CrI monolayers change upon adsorption of 3d transition-metal (TM) atoms (from Sc to Zn). Our results indicate that the electronic properties of the TM-CrI system can be tuned from semiconductor to metal/half-metal/spin gapless semiconductor depending on the choice of the adsorbed TM atoms. Moreover, the adsorption can improve the ferromagnetic stability of CrI monolayers by increasing both magnetic moments and . Notably, of CrI with Sc and V adatoms can be increased by nearly a factor of 3. We suggest postsynthesis doping of 2D CrI by deposition of TM atoms as a new route toward potential applications of TM-CrI systems in nanoelectronic and spintronic devices.
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http://dx.doi.org/10.1021/acsami.1c01701DOI Listing
April 2021

Analysis of the Cd(II) Adsorption Performance and Mechanisms by Soybean Root Biochar: Effect of Pyrolysis Temperatures.

Bull Environ Contam Toxicol 2021 Apr 20. Epub 2021 Apr 20.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, People's Republic of China.

As one of the most harmful environmental pollutants, cadmium (Cd) has arisen much interest, and many researches have been carried out to study the adsorption of heavy metals by biochar, but the mechanisms were poorly explored and the roles components in biochar played are still indistinct. In this study, we evaluated the adsorption capacities and mechanisms of soybean root biochar pyrolyzed at four different temperatures. The results indicate the biochar properties are significantly determined by pyrolysis temperature, which affects the removal mechanisms of Cd(II) consequently. Microstructure characteristics and mechanism analysis further suggest that Cd(II)-π interactions and sulfur-containing functional groups are the main mechanisms of Cd(II) adsorption. This work shows a new perspective to explain the adsorption mechanisms onto biochar adsorbents and has a benefit for the exploitation of economical and effective adsorbents for Cd(II) removal based on biochars.
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http://dx.doi.org/10.1007/s00128-021-03235-2DOI Listing
April 2021

Complete genome sequence analysis of the peanut pathogen Ralstonia solanacearum strain Rs-P.362200.

BMC Microbiol 2021 Apr 19;21(1):118. Epub 2021 Apr 19.

College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Background: Bacterial wilt caused by Ralstonia solanacearum species complex is an important soil-borne disease worldwide that affects more than 450 plant species, including peanut, leading to great yield and quality losses. However, there are no effective measures to control bacterial wilt. The reason is the lack of research on the pathogenic mechanism of bacterial wilt.

Results: Here, we report the complete genome of a toxic Ralstonia solanacearum species complex strain, Rs-P.362200, a peanut pathogen, with a total genome size of 5.86 Mb, encoding 5056 genes and the average G + C content of 67%. Among the coding genes, 75 type III effector proteins and 12 pseudogenes were predicted. Phylogenetic analysis of 41 strains including Rs-P.362200 shows that genetic distance mainly depended on geographic origins then phylotypes and host species, which associated with the complexity of the strain. The distribution and numbers of effectors and other virulence factors changed among different strains. Comparative genomic analysis showed that 29 families of 113 genes were unique to this strain compared with the other four pathogenic strains. Through the analysis of specific genes, two homologous genes (gene ID: 2_657 and 3_83), encoding virulence protein (such as RipP1) may be associated with the host range of the Rs-P.362200 strain. It was found that the bacteria contained 30 pathogenicity islands and 6 prophages containing 378 genes, 7 effectors and 363 genes, 8 effectors, respectively, which may be related to the mechanism of horizontal gene transfer and pathogenicity evaluation. Although the hosts of HA4-1 and Rs-P.362200 strains are the same, they have specific genes to their own genomes. The number of genomic islands and prophages in HA4-1 genome is more than that in Rs-P.36220, indicating a rapid change of the bacterial wilt pathogens.

Conclusion: The complete genome sequence analysis of peanut bacterial wilt pathogen enhanced the information of R. solanacearum genome. This research lays a theoretical foundation for future research on the interaction between Ralstonia solanacearum and peanut.
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http://dx.doi.org/10.1186/s12866-021-02157-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056632PMC
April 2021

Impact of heat stress and a feed supplement on hormonal and inflammatory responses of dairy cows.

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

Department of Animal and Dairy Science, University of Georgia, Tifton 31793. Electronic address:

The aim of this trial was to evaluate the effects of an immunomodulatory supplement (OmniGen AF, OG; Phibro Animal Health Corp.) and heat stress on hormonal, inflammatory, and immunological responses of lactating dairy cows. Sixty multiparous Holstein cows were randomly assigned to 4 treatments in a 2 × 2 factorial arrangement using 2 environments: cooled using fans and misters, or noncooled, and 2 top-dressed feed supplements (56 g/d): OG or a placebo (CTL). Temperature-humidity index averaged 78 during the 8-wk trial. Blood was drawn to analyze cortisol, prolactin, and circulating tumor necrosis factor (TNF)-α and IL10. Peripheral blood mononuclear cells (PBMC) were isolated and stimulated with hydrocortisone, prolactin, or lipopolysaccharide (LPS), individually or in several combinations, to assess induced proliferation and cytokine production. At d 52, 6 cows per treatment were injected i.v. with an LPS bolus (ivLPS) to assess hormone and cytokine responses. For cooled cows, feeding OG increased plasma cortisol concentration relative to CTL. Noncooled cows fed CTL had lower circulating TNF-α concentrations than noncooled-OG and cooled-CTL cows, with cooled-OG intermediate. Hydrocortisone+LPS-stimulated PBMC from OG cows tended to proliferate more than CTL. Relative to cooled cows, PBMC from noncooled cows produced more TNF-α and IL10 when stimulated with LPS. Following ivLPS, cooled-OG cows had a greater cortisol response than the other treatments. In conclusion, OG supplementation enhanced cortisol release under basal condition and induced inflammation with cooling compared with CTL. This suggests that heat stress inhibits OG-mediated cortisol release. Heat stress seemed to enhance the inflammatory responses of PBMC from lactating cows. However, OG supplementation promoted PBMC proliferation under stress, or in the presence of hydrocortisone.
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http://dx.doi.org/10.3168/jds.2021-20162DOI Listing
April 2021

Retrospective analysis of the incidence and predictive factors of parametrial involvement in FIGO IB1 cervical cancer.

J Gynecol Obstet Hum Reprod 2021 Apr 10;50(8):102145. Epub 2021 Apr 10.

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, China. Electronic address:

Background And Objectives: Radical surgery is the standard primary treatment for patients with stage IB1 (FIGO 2009 staging) cervical cancer due to latent parametrial involvement. Recent studies suggested that less radical surgery was applicable for patients with no or low risk of parametrial involvement. In this study, we aimed to determine the incidence and possible predictive factors of parametrial involvement in patients with stage IB1 cervical cancer so as to evaluate whether less radical surgery was suitable for selected patients.

Methods: Clinical data of patients who underwent type C radical hysterectomy with pelvic lymphadenectomy and diagnosed as stage IB1 cervical cancer at Union Hospital, Wuhan, China from October 2014 to December 2017 were collected and analysed retrospectively. The incidence of parametrial involvement was calculated and the risk factors for parametrial involvement were evaluated by univariate and multivariate logistic regression.

Results: Among 282 eligible patients, 33 (11.7%) had parametrial involvement. Postmenopause, lymphovascular space invasion (LVSI), lymph node metastasis (LNM), deep stromal invasion (outer 1/3) and tumor size larger than 2 cm were statistically associated with parametrial involvement. Multivariate analysis showed that LNM (OR = 11.431; 95%CI: 3.455 - 37.821), deep stromal invasion (OR = 6.080; 95%CI: 1.814 - 20.382) and LVSI (OR = 7.147; 95%CI: 1.863-27.411) remained as independent risk factors for parametrial involvement in patients with stage IB1 cervical cancer.

Conclusions: The incidence of parametrial involvement in stage IB1 cervical cancer is non-negligible. Only LNM, LVSI and deep stromal invasion were independent predictors, which were not easy to evaluate accurately before surgery. Less radical surgery requires modified pre-treatment evaluation methods and prospective data support.
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http://dx.doi.org/10.1016/j.jogoh.2021.102145DOI Listing
April 2021

Efficacy of Electroacupuncture Combined with Tropisetron in Treating Carboprost Tromethamine-Induced Nausea and Vomiting during Cesarean Section under Lumbar Anesthesia.

Complement Med Res 2021 Mar 30:1-7. Epub 2021 Mar 30.

Department of Anesthesiology, Cangzhou Central Hospital, Cangzhou, China.

Objective: We evaluated the efficacy of electroacupuncture combined with tropisetron in treating carboprost tromethamine-induced nausea and vomiting during cesarean section under lumbar anesthesia.

Methods: A total of 264 patients aged 22-40 years were enrolled, who received carboprost tromethamine and suffered nausea and vomiting during cesarean section under lumbar anesthesia. The patients were divided randomly into the control group, electroacupuncture group, tropisetron group, and electroacupuncture + tropisetron group.

Results: Compared to the control group, the nausea and vomiting scores decreased at T3 in both the electroacupuncture and electroacupuncture + tropisetron groups, and decreased at T4 in the electroacupuncture group, tropisetron group, and electroacupuncture + tropisetron group; the motilin, gastrin, and 5-hydroxytryptamine (5-HT) levels decreased at T5 in the other 3 groups. Compared to the electroacupuncture + tropisetron group, the nausea and vomiting scores increased at T3 in the control and tropisetron groups, and increased at T4 in the other 3 groups; the motilin, gastrin, and 5-HT levels increased at T5.

Conclusions: Our study suggested that electroacupuncture combined with tropisetron could effectively relieve carboprost tromethamine-induced nausea and vomiting during cesarean section under lumbar anesthesia. The effect was better than its single application, and the reduced 5-HT, motilin, and gastrin levels might be involved in the underlying mechanism.
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http://dx.doi.org/10.1159/000514946DOI Listing
March 2021

Introducing hydroxyl groups as cellulose-binding sites into polymeric solid acids to improve their catalytic performance in hydrolyzing cellulose.

Carbohydr Polym 2021 Jun 5;261:117895. Epub 2021 Mar 5.

Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI, 53706, United States. Electronic address:

Effective hydrolysis of cellulose to glucose is a crucial step to produce fuels and chemicals from lignocellulosic biomass. Solid acids are promising alternatives of cellulases and homogenous acids for hydrolyzing cellulose. In this study, porous polymeric solid acids bearing hydroxyl and sulfonic acid groups were fabricated for cellulose hydrolysis in water through the low-cost Friedel-Crafts "knitting" polymerization of hydroxyl-containing aromatic monomers followed by sulfonation. The synthesized bifunctional solid acids could effectively hydrolyze microcrystalline cellulose (Avicel) to glucose by as high as 93 % at 120 °C within 48 h and ball-milled Avicel by 98 % at 120 °C in 24 h. The evidence from this study indicated that the outstanding catalytic performance of the solid acids was attributed to the porous structure (large surface area) and the presence of the hydroxyl (cellulose-binding group) in the solid acids.
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http://dx.doi.org/10.1016/j.carbpol.2021.117895DOI Listing
June 2021

Heterogeneous Interface Design to Enhance the Photocatalytic Performance.

Inorg Chem 2021 Apr 25;60(7):5063-5070. Epub 2021 Mar 25.

Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology, Mianyang 621010, China.

Heterojunction photocatalysts, which can relieve the low carrier separation efficiency and insufficient light absorption ability of one catalyst, have received extensive attention. To construct an ideal heterojunction for photocatalysis, most previous studies focused on energy band structure engineering to prolong charge carrier lifetime and increase the reaction rates, which are critical to increase the photocatalytic activity. Here, the heterojunction interface was surprisingly found to be another important factor to affect the photocatalytic performance. We design three heterojunction interface models of α-FeO/BiO, corresponding to "ring-to-face", "face-to-face", and "rod-to-face". By tuning the heterogeneous interfaces, the photocatalytic performance of composites was significantly improved. On the basis of the type I energy band structures, the optimized face-to-face model realized a photocatalytic efficiency of 90.8% that of pure α-FeO (<30%) for degradation of methylene blue and a higher efficiency (80%) for degrading tetracycline within 60 min, which were superior to most Fe/Bi/O-based photocatalytic heterojunctions. Furthermore, the results disclosed that the enhanced performance was owing to the sufficient interfacial contact and low interfacial resistance of the face-to-face model, which provided sufficient channels for efficient charge transfer. This work offers a new direction of tuning heterojunction interface for designing composite photocatalysts.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00093DOI Listing
April 2021

Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).

Biochem J 2021 Apr;478(8):1571-1583

Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, U.S.A.

The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems. Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation. In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end. We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones. We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm. FUT8 could also fucosylate paucimannose structures when they are on glycoprotein substrates. Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers. We also found that sialylation of N-glycans significantly reduced their activity as a substrate of FUT8. Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation. Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.
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http://dx.doi.org/10.1042/BCJ20210138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062310PMC
April 2021

Substrate Topography Regulates Differentiation of Annulus Fibrosus-Derived Stem Cells via CAV1-YAP-Mediated Mechanotransduction.

ACS Biomater Sci Eng 2021 03 30;7(3):862-871. Epub 2020 Mar 30.

Department of Orthopaedic Surgery, Orthopaedic Institute, The First Affiliated Hospital, Medical College, Soochow University, Suzhou, Jiangsu 215007, China.

Regeneration of annulus fibrosus (AF) through tissue engineering techniques shows promise as a treatment for patients with degenerative disc disease (DDD). Yet, it remains challenging because of the intrinsic heterogeneity of AF tissue and shortage of in-depth knowledge of its structure-function correlation. In the current study, we fabricated fibrous poly(ether carbonate urethane)urea (PECUU) scaffolds with various fiber sizes to mimic the microstructural feature of native AF and aimed to regulate the differentiation of AF-derived stem cells (AFSCs) by controlling the topographical cues of the scaffold. We found that the morphology of AFSCs varied significantly on scaffolds with various fiber sizes. Meanwhile, the expression of the phenotypic marker genes of outer AF was up-regulated on scaffolds with large fibers. Meanwhile, enhanced expression of the phenotypic marker genes of inner AF was seen on scaffolds with small fibers. Such topography-dependent gene expression in AFSCs approximated the biochemical profile of AF tissue in various zones. Moreover, cell spreading and nucleus translocation of Yes-associated protein (YAP) were facilitated with increased fiber size. Formation and maturation of focal adhesions of AFSCs were also promoted. We also found that Caveolin-1 (CAV1) positively modulated the mechano-responses of YAP in response to substrate topography. In conclusion, depending on the activation of the CAV1-YAP mechanotransduction axis, tuning the fiber size of scaffolds can effectively induce changes in cell shape, adhesions, and extracellular matrix expression. This work may therefore provide new insights in the design of novel materials toward AF tissue regeneration.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01823DOI Listing
March 2021

Self-Assembled DNA Nanostructure as a Carrier for Targeted siRNA Delivery in Glioma Cells.

Int J Nanomedicine 2021 3;16:1805-1817. Epub 2021 Mar 3.

Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, People's Republic of China.

Introduction: RNA interference is a promising therapy in glioma treatment. However, the application of RNA interference has been limited in glioma therapy by RNA instability and the lack of tumor targeting. Here, we report a novel DNA tetrahedron, which can effectively deliver small interfering RNA to glioma cells and induce apoptosis.

Methods: siRNA, a small interfering RNA that can suppress the expression of survivin in glioma, was loaded into the DNA tetrahedron (TDN). To enhance the ability of active targeting of this nanoparticle, we modified one side of the DNA nanostructure with aptamer as1411 (As-TDN-R), which can selectively recognize the nucleolin in the cytomembrane of tumor cells. The modified nanoparticles were characterized by agarose gel electrophoresis, dynamic light scattering, and transmission electron microscopy. The serum stability was evaluated by agarose gel electrophoresis. Nucleolin was detected by Western blot and immunofluorescence, and targeted cellular uptake was examined by flow cytometry. The TUNEL assay, flow cytometry, and Western Blot were used to detect apoptosis in U87 cells. The gene silencing of survivin was examined by qPCR, Western Blot, and immunofluorescence.

Results: As-TDN-R alone showed better stability towards siRNA, indicating that TDN was a good siRNA protector. Compared with TDN alone, there was increased intercellular uptake of As-TDN-R by U87 cells, evidenced by overexpressed nucleolin in glioma cell lines. TUNEL assay, flow cytometry, and Western Blot revealed increased apoptosis in the As-TDN-R group. The downregulation of survivin protein and mRNA expression levels indicated that As-TDN-R effectively silenced the target gene.

Conclusion: The novel nanoparticle can serve as a good carrier for targeting siRNA delivery in glioma. Further exploration of the DNA nanostructure can greatly promote the application of DNA-based drug systems in glioma.
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http://dx.doi.org/10.2147/IJN.S295598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938230PMC
March 2021

The Relationship Between Fear of COVID-19 and Online Aggressive Behavior: A Moderated Mediation Model.

Front Psychol 2021 15;12:589615. Epub 2021 Feb 15.

School of Education, Jiangxi Normal University, Nanchang, China.

Amid the COVID-19 pandemic, fear has run rampant across the globe. To curb the spread of the virus, several governments have taken measures to drastically transition businesses, work, and schooling to virtual settings. While such transitions are warranted and well-intended, these measures may come with unforeseen consequences. Namely, one's fear of COVID-19 may more readily manifest as aggressive behaviors in an otherwise incognito virtual social ecology. In the current research, a moderated mediation model examined the mechanisms underlying the relation between fear of COVID-19 and overt and relational aggressive online behavior among Chinese college students. Utilizing a large sample of Chinese college students ( = 2,799), results indicated that moral disengagement mediated the effect of fear of COVID-19 on college students' overt and relational online aggressive behavior. A positive family cohesion buffered the effect of moral disengagement on relational aggressive behavior, but only for females. The findings, theoretical contributions, and practical implications of the present paper are also discussed.
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http://dx.doi.org/10.3389/fpsyg.2021.589615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917050PMC
February 2021

Percutaneous endoscopic lumbar discectomy compared with other surgeries for lumbar disc herniation: A meta-analysis.

Medicine (Baltimore) 2021 Mar;100(9):e24747

Department of Spine Surgery, Tianjin Hospital, Tianjin.

Objective: This meta-analysis was performed to investigate whether percutaneous endoscopic lumbar discectomy (PELD) had a superior effect than other surgeries in the treatment of patients with lumbar disc herniation (LDH).

Method: We searched PubMed, Embase, and Web of Science through February 2018 to identify eligible studies that compared the effects and complications between PELD and other surgical interventions in LDH. The outcomes included success rate, recurrence rate, complication rate, operation time, hospital stay, blood loss, visual analog scale (VAS) score for back pain and leg pain, 12-item Short Form Health Survey (SF12) physical component score, mental component score, Japanese Orthopaedic Association Score, Oswestry Disability Index. A random-effects or fixed-effects model was used to pool the estimate, according to the heterogeneity among the included studies.

Results: Fourteen studies (involving 2,528 patients) were included in this meta-analysis. Compared with other surgeries, PELD had favorable clinical outcomes for LDH, including shorter operation time (weight mean difference, WMD=-18.14 minutes, 95%CI: -25.24, -11.05; P < .001) and hospital stay (WMD = -2.59 days, 95%CI: -3.87, -1.31; P < .001), less blood loss (WMD = -30.14 ml, 95%CI: -43.16, -17.13; P < .001), and improved SF12- mental component score (WMD = 2.28, 95%CI: 0.50, 4.06; P = .012)) and SF12- physical component score (WMD = 1.04, 95%CI: 0.37, 1.71; P = .02). However, it also was associated with a significantly higher rate of recurrent disc herniation (relative risk [RR] = 1.65, 95%CI: 1.08, 2.52; P = .021). There were no significant differences between the PELD group and other surgical group in terms of success rate (RR = 1.01, 95%CI: 0.97, 1.04; P = .733), complication rate (RR = 0.86, 95%CI: 0.63, 1.18; P = .361), Japanese Orthopaedic Association Score score (WMD = 0.19, 95%CI: -1.90, 2.27; P = .861), visual analog scale score for back pain (WMD = -0.17, 95%CI: -0.55, 0.21; P = .384) and leg pain (WMD = 0.00, 95%CI: -0.10, 0.10; P = .991), and Oswestry Disability Index score (WMD = -0.29, 95%CI: -1.00, 0.43; P = .434).

Conclusion: PELD was associated with better effects and similar complications with other surgeries in LDH. However, it also resulted in a higher recurrence rate. Considering the potential limitations in the present study, further large-scale, well-performed randomized trials are needed to verify our findings.
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http://dx.doi.org/10.1097/MD.0000000000024747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939231PMC
March 2021

The production, detection, and origin of irisin and its effect on bone cells.

Int J Biol Macromol 2021 May 27;178:316-324. Epub 2021 Feb 27.

Department of Spine Surgery, Tianjin Hospital, 406 Jiefang South Road, Hexi District, Tianjin 300211, China. Electronic address:

Irisin is a muscle factor discovered in 2012 that plays an important role in many tissues, including bone. Eight years since its discovery, there are still many controversies regarding its molecular biology, detection, and effects on bone. This article summarizes the points raised to date, and discusses the mechanisms by which irisin regulates bone cells. The information reviewed here provides a useful foundation for future research.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.02.181DOI Listing
May 2021

Study on microbial communities and higher alcohol formations in the fermentation of Chinese Xiaoqu Baijiu produced by traditional and new mechanical technologies.

Food Res Int 2021 Feb 10;140:109876. Epub 2020 Nov 10.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address:

Microorganisms play an important role in the flavor formation of Chinese Baijiu. Mechanization is the way to develop Baijiu. Therefore, it is necessary to study the effects of mechanization on microbial community and flavor in Baijiu production. The microbial communities exhibited differences between two technologies with the fermentation, and the numbers of yeasts and bacteria in new mechanical technology were significantly higher than those in traditional technology at the peak of fermentation. Both metagenomic and metatranscriptomic analyses showed 5 core microorganisms in the fermentation, namely Saccharomyces cerevisiae, Rhizopus delemar, Pichia kudriavzevii, Lactobacillus helveticus, and Rhizopus oryzae. S. cerevisiae was generally regarded to be the most dominant yeast in Baijiu fermentation, but our metatranscriptomic data showed that P. kudriavzevii was more active than S. cerevisiae. These two analyses indicated that higher initiation abundance of S. cerevisiae and P. kudriavzevii and lower initiation abundance of R. delemar and R. oryzae were observed in traditional technology than in new technology, and that Lactobacillus displayed apparent advantages in traditional technology, whereas Lactobacillus and yeast exhibited obvious advantages in new technology at the end of fermentation. In addition to S. cerevisiae, other microorganisms including non-saccharomyces yeasts, molds, and bacteria were involved in higher alcohol formation. This work provides insight into the microbial dynamics and higher alcohol formation, as well as an efficient strategy for process improvement in Baijiu fermentation.
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http://dx.doi.org/10.1016/j.foodres.2020.109876DOI Listing
February 2021

A review of treatment technologies for produced water in offshore oil and gas fields.

Sci Total Environ 2021 Jun 30;775:145485. Epub 2021 Jan 30.

School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address:

Offshore oil and gas production is increasingly growing popular globally. Produced water (PW), which is the largest byproduct of oil and gas production, is a complex mixture of dissolved and undissolved organic and inorganic substances. PW contributes considerably to oil pollution in the offshore petroleum and gas industry owing to the organic substances, which mainly include hydrocarbons; this is a major concern to researchers because of the long-term adverse effects on the ecosystem. Since the development of offshore petroleum and gas industry, the PW treatment process has been classified into pretreatment, standard-reaching treatment, and advanced purification treatment based on the characteristics of PW and has been coupled with the environmental, economic, and regulatory considerations. The mechanism, design principle, application, and development of conventional technologies for PW treatment, such as gravity and enhanced gravity sedimentation, hydrocyclone, gas flotation, and medium filtration, are summarized in this study. Novel methods for further application, such as tubular separation, combined fibers coalescence, and membrane separation, are also discussed. Enhancement of treatment with multiple physical fields and environmentally friendly chemical agents, coupled with information control technology, would be the preferred PW treatment approach in the future. Moreover, the PW treatment system should be green, efficient, secure, and intelligent to satisfy the large-scale, unmanned, and abyssal exploration of offshore oil and gas production in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145485DOI Listing
June 2021

A graphene-coated silk-spandex fabric strain sensor for human movement monitoring and recognition.

Nanotechnology 2021 Feb 18. Epub 2021 Feb 18.

College of Electrical Engineering, Zhejiang University, Hangzhou, Zhejiang, CHINA.

Flexible and stretchable sensors are emerging and promising wearable devices for human motion monitoring. Manufacturing a flexible and stretchable strain sensor with desirable electromechanical performance and excellent skin compatibility plays an important role in building a smart wearable system. In this paper, a graphene-coated silk-spandex (GCSS) fabric strain sensor is prepared by reducing graphene oxide (GO). The sensor functions as a result of conductive fiber extending and woven structure deforming. The conductive fabric can be stretched towards 60% with high sensitivity, and its performance remains constant after a 1000-cycle test. Based on its superior performance, the GCSS is successfully employed for detecting full-range human movement and can provide data for a deep learning based gesture recognition. This work offers a desirable method to fabricate low-cost strain sensors for industrial applications such as human movement detection and advanced information science.
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http://dx.doi.org/10.1088/1361-6528/abe788DOI Listing
February 2021

Multi-Channel Data Acquisition Card under New Acquisition and Transmission Architecture of High Frequency Ground Wave Radar.

Sensors (Basel) 2021 Feb 5;21(4). Epub 2021 Feb 5.

School of Information Science and Engineering, Harbin Institute of Technology at Weihai, Weihai 264209, China.

It is known that the data acquisition and processing system plays an important role in radar target detection system. In order to meet the requirements of real-time processing and accurate transmission of echo signals in high-frequency ground-wave radar (HFGWR) systems, a new acquisition and transmission framework utilizing the designed acquisition card based on the PCIe (peripheral component interconnect express) has been designed and is presented in this paper. The Xilinx FPGA (Field-Programmable Gate Array) chip Kintex7-XC7K325T is adopted as a hardware carrier in acquisition card. The hardware's composition, analog front-end circuit, the DMA (Direct Memory Access) transmission, FPGA structure, ADC (Analog-to-Digital Converter) chip, and performance test of this card are showed and discussed. Currently, the acquisition card has been accomplished and applied in the practical system of HFGWR.
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http://dx.doi.org/10.3390/s21041128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915965PMC
February 2021

Fiber coalescence treatment of oily wastewater: A new theory and application.

J Hazard Mater 2021 Jun 21;412:125188. Epub 2021 Jan 21.

National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address:

Oil pollution from produced water in the offshore petroleum industry is one of the most serious marine pollutants worldwide, and efficient separation technology is crucial for the control of oil pollutant emission. Medium coalescence is an efficient oil-water separation technology, but its theory is lacking and the development is slow. In this work, the microscopic mechanism of fiber coalescence was revealed, and found that the effective collision positions were the three-phase contact line and the exposed fiber surface. Further, a theoretical model for calculating the separation performance of a fiber bed was established. For a given inlet droplet size distribution and bed geometric parameters, the outlet droplet size distribution and the total separation efficiency of the fiber bed can be predicted. Then, an Ω-shaped woven method composed of oil-wet fibers and oil-phobic fibers was designed and the separation performance of the fiber beds prepared by the method and the influence law of various parameters were clarified through macroscopic experiment. Finally, the novel technology achieved its first engineering application on an offshore platform, with the average oil content of the outlet was less than 25 mg/L, which could reform the current treatment process of produced water.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125188DOI Listing
June 2021

Abundance and mobility of metal(loid)s in reservoir sediments of Singe Tsangpo and Yarlung Tsangpo in Tibet, China: Implications for ecological risk.

Environ Geochem Health 2021 Feb 4. Epub 2021 Feb 4.

School of Environmental Science and Engineering, Southern University of Science and Technology of China, Shenzhen, 518055, China.

Geogenic arsenic enrichment in soil and river sediments of Tibet compared to its upper crustal abundance has been observed, raising the question whether other trace elements are also enriched and thus may pose ecological risks. Because human activities are limited, the reservoir sediments after the recent construction of the Shiquan dam on the Singe Tsangpo (ST) and the Zam dam on the Yarlung Tsangpo (YT) collect and thus represent material sourced from 14,870 km and 157,668 km of drainage areas, respectively. Bulk concentrations of the metalloid (As) and 13 metals (Li, Be, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, Cs, and Pb) are analyzed for 123 samples from 9 mostly silty sediment cores (depth: 11-20 cm) from the Shiquan Reservoir and for 250 samples from 13 mostly sandy sediment cores (depth: 9-28 cm) from the Zam Reservoir. These elemental concentrations are normalized to the upper crustal Fe abundance of 3.9% to arrive at a regional sediment geochemical background value for each element. The regional background values of most elements in the ST drainage and the YT drainage are comparable with the upper crustal abundance. However, three elements (Li, As, and Cs) in both drainage basins display significant enrichment compared to their respective upper crustal abundance. Sequential leaching of a subset of sediment samples from the ST (n = 18) and YT (n = 29) drainages reveals that chemical fractions of metals and metalloids in these two reservoirs are similar, with most of the elements dominated by the residual fraction with low mobility. Taken together, the ecological risks of the most studied elements in the reservoir sediments are likely low pending further aquatic bioavailability investigations, except that As, Cu, Pb, and Be deserve more attention due to their elevated levels in mobile fractions.
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http://dx.doi.org/10.1007/s10653-021-00810-8DOI Listing
February 2021

Exploring the risk-taking tendency among migrant workers in the COVID-19 pandemic: The role of ontological security.

Work 2021 ;68(2):269-283

School of Economics and Management, Southwest Jiaotong University, Chengdu, China.

Background: The COVID-19 pandemic has changed the social environment of most laborers around the world and has profoundly affected people's ontological security and behavior choices. Among them, the migrant workers are one of the groups most affected by the pandemic.

Objective: This study explored the mechanism of the impact of the scarcity of ontological security caused by the pandemic on the risk-taking tendency of migrant workers in China through two studies.

Methods: This study adopts two experimental method, with 514 participants in the first study and 357 participants in the second study.

Results: The results show that the pandemic-induced scarcity perception of ontological security promotes their risk-taking tendency, and the migrant workers' cognitive reflection ability, sense of unfairness and expected benefits play a significant mediating role in this process. The scarcity perception of ontological security promotes migrant workers' risk-taking tendency by reducing the cognitive reflection ability, triggering the sense of unfairness and overstating expected benefits.

Conclusions: The conclusion of this study can help migrant workers, enterprises and government to avoid potential workplace and social bad behavior.
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http://dx.doi.org/10.3233/WOR-205017DOI Listing
March 2021

Exploring a combined Escherichia coli-based glycosylation and in vitro transglycosylation approach for expression of glycosylated interferon alpha.

Bioorg Med Chem 2021 Mar 22;33:116037. Epub 2021 Jan 22.

Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA. Electronic address:

The conventional use of E. coli system for protein expression is limited to non-glycosylated proteins. While yeast, insect and mammalian systems are available to produce heterologous glycoproteins, developing an engineered E. coli-based glycosylation platform will provide a faster, more economical, and more convenient alternative. In this work, we present a two-step approach for production of a homogeneously glycosylated eukaryotic protein using the E. coli expression system. Human interferon α-2b (IFNα) is used as a model protein to illustrate this glycosylation scheme. In the first step, the N-glycosyltransferase from Actinobacillus pleuropneumoniae (ApNGT) is co-expressed for in vivo transfer of a glucose residue to IFNα at an NX(S/T) N-glycosylation sequon. Several E. coli systems were examined to evaluate the efficiency of IFNα N-glucosylation. In the second step, the N-glucosylated protein is efficiently elaborated with biantennary sialylated complex-type N-glycan using an in vitro chemoenzymatic method. The N-glycosylated IFNα product was found to be biologically active and displayed significantly improved proteolytic stability. This work presents a feasible E. coli-based glycosylation machinery for producing therapeutic eukaryotic glycoproteins.
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http://dx.doi.org/10.1016/j.bmc.2021.116037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923244PMC
March 2021

Full Endoscopic Lumbar Foraminoplasty with Periendoscopic Visualized Trephine Technique for Lumbar Disc Herniation with Migration and/or Foraminal or Lateral Recess Stenosis.

World Neurosurg 2021 Apr 27;148:e658-e666. Epub 2021 Jan 27.

Department of Spine Surgery, Tianjin Hospital, Tianjin University, Tianjin, China. Electronic address:

Purpose: To introduce a newly designed periendoscopic visualized trephine system for foraminoplasty in treating lumbar disc herniation with migration and/or foraminal or lateral recess stenosis, and report early clinical outcomes evaluated by the Patient-Reported Outcomes Measurement Information System (PROMIS).

Methods: A total of 25 patients who underwent transforaminal endoscopic lumbar discectomy with foraminoplasty using a periendoscopic visualized trephine from June 2019 to January 2020 were retrospectively reviewed. PROMIS pain interference and physical function were selected as outcome measures.

Results: The average age of the 25 patients (16 males, 9 females) was 32.0 ± 7.5 years (20-48 years). All patients were successfully followed up with the mean time of 10.1 ± 2.8 months (6-12 months). PROMIS pain interference scores decreased significantly from mean 67.0 ± 3.4 preoperatively to 37.5 ± 5.4 at the final follow-up (P < 0.01), and PROMIS physical function scores improved significantly from mean 29.2 ± 5.5 preoperatively to 59.3 ± 3.7 at the final follow-up (P < 0.01). No neural or vascular complication occurred.

Conclusion: Full endoscopic lumbar foraminoplasty with a periendoscopic visualized trephine technique is safe and effective for treating lumbar disc herniation with migration and/or lateral recess or foraminal stenosis, with improved flexibility and convenience and decreased radiation exposure.
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http://dx.doi.org/10.1016/j.wneu.2021.01.062DOI Listing
April 2021

Decompressive Hemicraniectomy Associated With Ultrasound-Guided Minimally Invasive Puncture and Drainage Has Better Feasibility Than the Traditional Hematoma Evacuation for Deteriorating Spontaneous Intracranial Hemorrhage in the Basal Ganglia Region: A Retrospective Observational Cohort Study.

Front Neurol 2020 12;11:561781. Epub 2021 Jan 12.

Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Spontaneous intracerebral hemorrhage (ICH) is a devastating disease with higher mortality and disability rates; however, ideal surgical management is still to be determined for critical ICH. The purpose of this study was to prove the feasibility and unique clinical value of a novel combination, decompressive hemicraniectomy associated with ultrasound-guided minimally invasive puncture and drainage (DH + MIPD), for deteriorating ICH in the basal ganglia region. According to the enrollment criteria, 168 ICH patients were analyzed retrospectively, of which 86 patients received DH + MIPD and 82 patients received DH associated with traditional hematoma evacuation as the control group. The change process of three parameters, including hematoma size, peri-hematoma edema, and intracranial pressure (ICP), in a period of time after operation, as well as the short- and long-term therapeutic effect, was compared. The DH + MIPD method could effectively achieve the evacuation rate of hematoma up to 87% at 5 days post-operation and had the significant advantages of minimal injury to cerebral tissue, less degree of edema, better effect of decreasing ICP, shorter operation time, less blood loss, and lower mortality compared with the control method. The DH + MIPD group had a significantly higher survival rate within 1 year post-operation ( = 0.007) and better functional outcome at 90 and 180 days post-operation ( = 0.004). A subgroup analysis pointed out that the DH + MIPD method had a definite survival advantage for critical ICH patients older than 60 years old and with hematoma located in the left dominant hemisphere. Our results proved the better feasibility of DH + MIPD on hematoma evacuation and implicated its significant advantages of reducing mortality and improving functional recovery. This method provides one more choice for the individualized therapy of ICH in the basal ganglia region.
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http://dx.doi.org/10.3389/fneur.2020.561781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835255PMC
January 2021

Genome-wide transcriptome profiling indicates the putative mechanism underlying enhanced grain size in a wheat mutant.

3 Biotech 2021 Feb 11;11(2):54. Epub 2021 Jan 11.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130 Sichuan China.

Grain size is an important trait for crops. The endogenous hormones brassinosteroids (BRs) play key roles in grain size and mass. In this study, we identified an ethyl methylsulfonate (EMS) mutant wheat line, , with increased grain size, 1000-grain weight, and protein content, but decreased starch content, compared with the levels in the wild type (WT). Comparative transcriptomic analysis of and WT at four developmental stages [9, 15, 20, and 25 days post-anthesis (DPA)] revealed a total of 264, 267, 771, and 1038 differentially expressed genes (DEGs) at these stages. Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis showed that some DEGs from the comparison at 15 DPA were involved in the pathway of "brassinosteroid biosynthesis," and eight genes involved in BR biosynthesis and signal transduction were significantly upregulated in during at least one stage. This indicated that the enhanced BR signaling in might have contributed to its increased grain size via network interactions. The expression of seed storage protein (SSP)-encoding genes in was upregulated, mostly at 15 and 20 DPA, while most of the starch synthetase genes showed lower expression in at all stages, compared with that in WT. The expression patterns of starch synthase genes and seed storage protein-encoding genes paralleled the decreased level of starch and increased storage protein content of , which might be related to the increased seed weight and wrinkled phenotype.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-020-02579-6.
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http://dx.doi.org/10.1007/s13205-020-02579-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801549PMC
February 2021

Role of the Calcified Cartilage Layer of an Integrated Trilayered Silk Fibroin Scaffold Used to Regenerate Osteochondral Defects in Rabbit Knees.

ACS Biomater Sci Eng 2020 02 10;6(2):1208-1216. Epub 2020 Jan 10.

Department of Spine Surgery, Tianjin Hospital, Tianjin University, 406 Jiefang Nan Road, Hexi District, Tianjin 300211, People's Republic of China.

The repair of osteochondral defects remains challenging, given the complexity of native osteochondral tissue and the limited self-repair capacity of cartilage. Osteochondral tissue engineering is a promising strategy. Here, we fabricated a biomimetic osteochondral scaffold using silk fibroin and hydroxyapatite, including a calcified cartilage layer (CCL). We studied the role played by the CCL in terms of cell viability in vivo. We established osteochondral defects in rabbit knees to investigate the effects of CCL-containing scaffolds with or without adipose tissue-derived stem cells (ADSCs). We evaluated osteochondral tissue regeneration by calculating gross observational scores, via histological and immunohistochemical assessments, by performing quantitative biochemical and biomechanical analyses of new osteochondral tissue, and via microcomputed tomography of new bone at 4, 8, and 12 weeks after surgery. In terms of surface roughness and integrity, the CCL + ADSCs group was better than the CCL and the non-CCL + ADSCs groups at all time points tested; the glycosaminoglycan and collagen type II levels of the CCL + ADSCs group were highest, reflecting the important role played by the CCL in cartilage tissue repair. Subchondral bone smoothness was better in the CCL + ADSCs group than in the non-CCL + ADSCs and CCL groups. The CCL promoted smooth subchondral bone regeneration but did not obviously affect bone strength or quality. In conclusion, a biomimetic osteochondral scaffold with a CCL, combined with autologous ADSCs, satisfactorily regenerated a rabbit osteochondral defect. The CCL enhances cartilage and subchondral bone regeneration.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01661DOI Listing
February 2020

Ultrasound-Mediated Long-Circulating Nanopolymer Delivery of Therapeutic siRNA and Antisense MicroRNAs Leads to Enhanced Paclitaxel Sensitivity in Epithelial Ovarian Cancer Chemotherapy.

ACS Biomater Sci Eng 2020 07 3;6(7):4036-4050. Epub 2020 Jun 3.

Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Epithelial ovarian cancer (EOC) is one of the leading malignant tumors that seriously threaten women's health. The development of new drugs or increasing the sensitivities of current chemotherapy drugs is critically needed. The purpose of this study was to assess the synergistic effects of two silencing RNAs [salt-inducible kinase 2 (SIK2) siRNA and antisense-microRNA21 (anti-miR21)] encapsulated in long-circulating folate-lipid-poly(lactic--glycolic acid) (PLGA) hybrid nanopolymers (FaLPHNPs) administered using an ultrasound- and microbubble (US-MB)-mediated approach to sensitize human EOC xenografts to paclitaxel (PTX). In the assays, this lipid-PLGA hybrid nanopolymer exhibited an extended circulation profile (: ∼8.5 h); US-MB-mediated complementary delivery of FaLPHNPs resulted in a significant reduction in EOC cell (OVCR3, A2780, and SKOV3) proliferation. , there was a 2.5-fold increase ( < 0.05) in RNA delivery in EOC xenografts, which resulted in a notable inhibition of tumor growth compared with that in the non-ultrasound-mediated and PTX alone-treated controls. We validated the therapeutic roles of SIK2, the target gene in treating advanced ovarian cancer, and anti-miR21 by evaluating the significant inhibition of tumor growth upon SIK2 silencing and inhibition of endogenous miR21 function. In summary, the results of this study revealed that US-MB-mediated codelivery of SIK2 siRNA, and anti-miR21 encapsulated in a folate-lipid-PLGA hybrid polymer nanoparticle could significantly improve the sensitivity of EOC tumors to PTX and is a highly effective approach for treating EOC in complementary experiments. Further research of this strategy could lead to better treatment results for patients with EOC.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00330DOI Listing
July 2020

Gene Therapy for Drug-Resistant Glioblastoma via Lipid-Polymer Hybrid Nanoparticles Combined with Focused Ultrasound.

Int J Nanomedicine 2021 8;16:185-199. Epub 2021 Jan 8.

Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China.

Background: Therapy for glioblastoma (GBM) has always been very challenging, not only because of the presence of the blood-brain barrier (BBB) but also due to susceptibility to drug resistance. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) has revolutionized gene editing technology and is capable of treating a variety of genetic diseases, including human tumors, but there is a lack of safe and effective targeting delivery systems in vivo, especially in the central nervous system (CNS).

Methods: Lipid-polymer hybrid nanoparticles (LPHNs-cRGD) were constructed for efficient and targeting delivery of CRISPR/Cas9 plasmids targeting O6-methylguanine-DNA methyltransferase (MGMT), a drug-resistance gene to temozolomide (TMZ). Focused ultrasound (FUS)-microbubbles (MBs) were used to non-invasively and locally open the BBB to further facilitate gene delivery into glioblastoma in vivo. The gene editing efficiency and drug sensitivity changes were evaluated both in vitro and in vivo.

Results: The gene-loaded LPHNs-cRGD were successfully synthesized and could protect pCas9/MGMT from enzyme degradation. LPHNs-cRGD could target GBM cells and mediate the transfection of pCas9/MGMT to downregulate the expression of MGMT, resulting in an increased sensitivity of GBM cells to TMZ. MBs-LPHNs-cRGD complexes could safely and locally increase the permeability of the BBB with FUS irradiation in vivo and facilitated the accumulation of nanoparticles at the tumor region in orthotopic tumor-bearing mice. Furthermore, the FUS-assisted MBs-LPHNs-cRGD enhanced the therapeutic effects of TMZ in glioblastoma, inhibited tumor growth, and prolonged survival of tumor-bearing mice, with a high level of biosafety.

Conclusion: In this work, we constructed LPHNs-cRGD for targeting delivery of the CRISPR/Cas9 system, in combination with FUS-MBs to open the BBB. The MBs-LPHNs-cRGD delivery system could be a potential alternative for efficient targeting gene delivery for the treatment of glioblastoma.
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http://dx.doi.org/10.2147/IJN.S286221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802796PMC
January 2021

Epidemic Rumination and Resilience on College Students' Depressive Symptoms During the COVID-19 Pandemic: The Mediating Role of Fatigue.

Front Public Health 2020 9;8:560983. Epub 2020 Dec 9.

School of Education, Jiangxi Normal University, Nanchang, China.

The restriction of numerous sectors of society and the uncertainty surrounding the development of the COVID-19 pandemic have resulted in adverse psychological states to college students isolated at home. In this study, we explored the mediating role of fatigue in the effects of epidemic rumination and resilience on depressive symptoms as well as how epidemic rumination and resilience may interact with one another. A large sample of Chinese college students ( = 1,293) completed measures on epidemic rumination, resilience, fatigue, and depressive symptoms. Results indicated depressive symptomology was positively predicted by epidemic rumination while negatively predicted by resilience. In both cases, fatigue partially mediated these effects and positively predicted depressive symptoms. Unexpectedly, epidemic rumination and resilience interacted in a manner where the effect of rumination on fatigue became stronger as resiliency increased. Theoretical and practical implications are provided to further interpret the results.
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http://dx.doi.org/10.3389/fpubh.2020.560983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755644PMC
January 2021

A New Posterior Extensor Attachment-Point Reconstruction Technique for Cervical Spondylotic Myelopathy Involving C2 Segment: Clinical Outcome and Safety.

J Neurol Surg A Cent Eur Neurosurg 2021 Mar 22;82(2):169-175. Epub 2020 Dec 22.

Department of Spine Surgery, Tianjin Hospital, Tianjin, China.

Background And Study Aim:  Cervical spondylotic myelopathy (CSM) is a common degenerative disease that mainly occurs in elder patients, leading to different degrees of neurological dysfunction. Spinal cord involvement is mainly distributed at the C3-C7 segments, but it may also involve up to the C2 level. This study aimed to assess the clinical efficacy and safety of open-door laminoplasty using a new extensor attachment-point reconstruction technique for treating CSM involving the C2 segment.

Patients And Methods:  Fifty-nine patients with CSM involving the C2 segment and undergoing open-door laminoplasty were included in this retrospective study. Based on the titanium plate used in the operation, patients were divided into two groups, a reconstructed titanium plate fixation (RPF) group ( = 28) and a conventional titanium plate fixation (CPF) group ( = 31). Improvements in neurological function, cervical range of motion (ROM), cervical curvature index (CCI), preservation of posterior cervical muscle mass, and axial symptoms were compared between the two groups.

Results:  There were no significant differences in operative time and intraoperative blood loss between the groups ( > 0.05). The Japanese Orthopaedic Association (JOA) score significantly increased in both groups postsurgery ( < 0.05); the neurological recovery rate was similar between the two groups (64.1 ± 13.3% vs. 65.9 ± 14.7%,  > 0.05). There was no significant loss of cervical ROM in either group ( > 0.05). The anteroposterior dural sac diameter at the C2 level was significantly enlarged in both groups ( < 0.05). Alternatively, CCI was significantly reduced in the CRP group ( < 0.05) but unchanged in the RPF group ( > 0.05). The cross-sectional area of the posterior cervical muscles was also significantly reduced in the CPF group ( < 0.05) but maintained in the RPF group ( > 0.05). Finally, axial symptoms were more severe in the CPF group than in the RPF group ( < 0.05).

Conclusion:  Laminoplasty is an effective surgical procedure for CSM involving the C2 segment. The reconstructed titanium plate achieved superior maintenance of cervical curvature and reduced both muscle atrophy and severity of axial symptoms compared with titanium conventional plates.
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http://dx.doi.org/10.1055/s-0040-1719102DOI Listing
March 2021