Publications by authors named "Qiang Wei"

1,025 Publications

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Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain.

Front Microbiol 2021 20;12:667084. Epub 2021 Apr 20.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

Porcine epidemic diarrhea virus (PEDV) is the major pathogen that causes diarrhea and high mortality in newborn piglets with devastating impact to the pig industry. Recombination and mutation are the main driving forces of viral evolution and genetic diversity of PEDV. In 2016, an outbreak of diarrhea in piglets occurred in an intensive pig farm in Central China. A novel PEDV isolate (called HNAY) was successfully isolated from clinical samples. Sequence analysis and alignment showed that HNAY possessed 21-nucleotide (nt) insertion in its S1 gene, which has never been reported in other PEDV isolates. Moreover, the sequence of the insertion was identical with the sequence fragment in PEDV N gene. Notably, the HNAY strain exhibited two unique mutations (T500A and L521Y) in the neutralizing epitopes of the S1 protein that were different from those of other PEDV variant strains and CV777-based vaccine strains. Additionally, PEDV HNAY might be derived from a natural recombination between two Chinese variant PEDV strains. Animal experiments demonstrated that HNAY displayed higher pathogenicity compared with two other clinical isolates. This study lays the foundation for better understanding of the genetic evolution and molecular pathogenesis of PEDV.
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http://dx.doi.org/10.3389/fmicb.2021.667084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093569PMC
April 2021

A comprehensive study of the effects of phthalates on marine mussels: Bioconcentration, enzymatic activities and metabolomics.

Mar Pollut Bull 2021 Apr 28;168:112393. Epub 2021 Apr 28.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, PR China; School of Marine Sciences, Ningbo University, Ningbo 315211, PR China. Electronic address:

In this study, marine mussels (Mytilus coruscus) were exposed to three typical PAEs (dimethyl phthalate [DMP], dibutyl phthalate [DBP] and di(2-ethylhexyl) phthalate [DEHP]) at a range of doses for different times to investigate the ecotoxicological effects. The accumulation of the three PAE congeners in M. coruscus exhibited the following trend: DEHP > DBP > DMP. The antioxidant response of mussel gonadal tissue was enhanced with increasing concentrations of PAEs. For the DBP and DEHP treatment groups, glutathione (GSH) worked in concert with antioxidant enzymes to protect cells against reactive oxygen species (ROS), while GSH played a prominent antioxidant role in the DMP-treated group. The metabolomics results revealed that PAE exposure disrupted the metabolic balance of mussels. Overall, PAEs affect the amino acid metabolism, lipid metabolism, energy metabolism, osmoregulation and nerve activities of mussels. Our results provide further insight into the toxicological effects of PAEs on marine organisms.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112393DOI Listing
April 2021

Retroperitoneal or transperitoneal approach in robot-assisted partial nephrectomy, which one is better?

Cancer Med 2021 May 1. Epub 2021 May 1.

Department of Urology, West China Hospital, Sichuan University, Chengdu, China.

Purpose: To systematically assess the perioperative outcomes of retroperitoneal (RP) and transperitoneal (TP) approaches in robot-assisted partial nephrectomy (RAPN), we conducted an updated meta-analysis.

Methods: A literature retrieval of multi-database including PubMed, Web of Science, Embase, Cochrane Library, and CNKI was performed to identify eligible comparative studies from the inception dates to January 2021. Perioperative outcomes included operative time (OT), estimated blood loss (EBL), warm ischemia time (WIT), postoperative length of stay (PLOS), positive surgical margin (PSM), and complications (major complications and overall complications). Outcomes of data were pooled and analyzed with Review Manager 5.4.1.

Results: Twenty-one studies involving a total of 2482 RP and 3423 TP approach RAPN patients met the inclusion criteria. Operating time (OT) (weighted mean difference [WMD] -16.60; 95% confidence interval [CI] -23.08, -10.12; p < 0.01) and PLOS (WMD -0.46 days; 95% CI -0.69, -0.23; p < 0.01) were shorter in RP-RAPN. Besides, lower EBL (WMD -21.67; 95% CI -29.74, -13.60; p < 0.05) was also found in RP-RAPN. Meanwhile, no significant differences were found in other outcomes.

Conclusions: RP-RARN was superior to TP-RAPN in patients undergoing RAPN in terms of OT, PLOS, and estimated blood loss. Besides these two approaches have no significant differences in PSMs or perioperative complications.
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http://dx.doi.org/10.1002/cam4.3888DOI Listing
May 2021

Associations between the dietary inflammatory index and urinary incontinence among women younger than 65 years.

Sci Rep 2021 Apr 29;11(1):9340. Epub 2021 Apr 29.

Department of Urology, Institute of Urology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, No. 37, Guoxue Alley, Chengdu, Sichuan, 610041, People's Republic of China.

The purpose of this study was to evaluate the association between dietary inflammatory index (DII) and urinary incontinence (UI) among a representative sample of the US women. We performed a cross-sectional analysis of women younger than 65 years using the 1999 to 2016 NHANES (National Health and Nutrition Examination Survey) populations. DII were calculated based on baseline dietary intake using 24-h dietary recalls. UI was determined and categorized by self-reported questions. Multivariable logistic regression models were used to assess the association between DII and UI. Stratified linear regression models were applied to test for interaction in prespecified subgroup of interest. A total of 13,441 women age between 20 and 65 years were included in the final analysis. Of these participants 3230 (24.03%) complained of urgency UI, 5276 (39.25%) complained of stress UI and 2028 (15.09%) complained of mixed UI. On multivariate analysis, analysis with DII categorized as quartiles revealed significantly increase odds of urgency UI in the most pro-inflammatory quartile compared to the most anti-inflammatory quartile (OR 1.24, 95% CI 1.07-1.44, P = 0.004 for trend) in full adjustment model. Similar results were observed in SUI (OR 1.14, 95% CI 1.00-1.30, P = 0.021 for trend) and MUI (OR 1.20, 95% CI 1.02-1.43, P = 0.022 for trend). More pro-inflammatory diets, as presented by higher DII scores are associated with an increased likelihood of UI in American women younger than 65 years. Further studies are needed to explore the possible physiological mechanism and evaluate the potential therapeutic implications.
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http://dx.doi.org/10.1038/s41598-021-88833-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084978PMC
April 2021

Levofloxacin Versus Ciprofloxacin in the Treatment of Urinary Tract Infections: Evidence-Based Analysis.

Front Pharmacol 2021 8;12:658095. Epub 2021 Apr 8.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Urinary tract infections (UTIs) are one of the most common bacterial infections acquired both in community and hospital. Fluoroquinolones, represented by levofloxacin and ciprofloxacin, are widely used for treatment of UTIs. However, it remains controversial for the comparison between the 2 drugs, which propelled us to conduct the first evidence-based research on this topic. To establish their relative efficacy and safety, we searched Pubmed, embase, and Web of Science for randomized controlled trials (RCTs) for UTIs. A total of 5 RCTs were finally included, involving 2,352 patients and a systematic review and meta-analysis were performed to compare the end-of-therapy and posttherapy clinical success rate, microbial eradication rate and adverse event rate. Jadad score and Review Manager 5.3.0 version were applied respectively to evaluate the study quality and heterogeneity. There was no significant difference between levofloxacin and ciprofloxacin group in end-of-therapy or posttherapy clinical success rate and microbial eradication rate ( > 0.05). As for adverse event rate, the 2 drugs were comparable and both safe for clinical use. Based on one included trial and pharmacological research, we raised hypothesis that levofloxacin was superior to ciprofloxacin for treatment of E. coli-induced chronic bacterial prostatitis (CBP) and it required a further study to prove it.
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http://dx.doi.org/10.3389/fphar.2021.658095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060646PMC
April 2021

Comparative evaluation of high-density polyethylene and polystyrene microplastics pollutants: Uptake, elimination and effects in mussel.

Mar Environ Res 2021 Apr 8;169:105329. Epub 2021 Apr 8.

College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China.

The high-density polyethylene (HDPE) and the polystyrene (PS), which are typical microplastic contaminants, are frequently detected in the environment and have potential hazard to environmental health. In this study, the accumulation, elimination, tissue distribution and potential effects of the HDPE and the PS in the mussels (Mytilus galloprovincialis) were evaluated. The HDPE and the PS were found in various tissues (digestive gland > gill > gonad ≈ muscle) with no difference in distribution patterns. The accumulation of the HDPE and the PS rapidly increased in the first 48 h exposure, and the accumulation of HDPE was higher than that of PS. After 144 h of elimination, most of the HDPE and the PS were cleared by mussels. In addition, the activities of superoxide dismutase (SOD), catalase (CAT) and the content of oxidized glutathione considerably increased, indicating that the HDPE and the PS induced oxidative stress and prevented oxidative damage in elimination. The metabolomic analysis suggested that exposure to HDPE and PS induced alterations in the metabolic profiles of mussel. Differential metabolites were involved in energy metabolism, lipid metabolism, tricarboxylic acid cycle and neurotoxic response., and Meanwhile, the PS had a lower effect on mussel metabolism during elimination, but the effect of HDPE was increased. Overall, this study elucidated that the HDPE and the PS caused adverse effects on the mussels and provided insights toward understanding the hazard of different microplastics on aquatic organisms.
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http://dx.doi.org/10.1016/j.marenvres.2021.105329DOI Listing
April 2021

Downregulation of miR-335 exhibited an oncogenic effect via promoting KDM3A/YAP1 networks in clear cell renal cell carcinoma.

Cancer Gene Ther 2021 Apr 23. Epub 2021 Apr 23.

Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cancer affecting many people worldwide. Although the 5-year survival rate is 65% in localized disease, after metastasis, the survival rate is <10%. Emerging evidence has shown that microRNAs (miRNAs) play a crucial regulatory role in the progression of ccRCC. Here, we show that miR-335, an anti-onco-miRNA, is downregulation in tumor tissue and inhibited ccRCC cell proliferation, invasion, and migration. Our studies further identify the H3K9me1/2 histone demethylase KDM3A as a new miR-335-regulated gene. We show that KDM3A is overexpressed in ccRCC, and its upregulation contributes to the carcinogenesis and metastasis of ccRCC. Moreover, with the overexpression of KDM3A, YAP1 was increased and identified as a direct downstream target of KDM3A. Enrichment of KDM3A demethylase on YAP1 promoter was confirmed by CHIP-qPCR and YAP1 was also found involved in the cell growth and metastasis inhibitory of miR-335. Together, our study establishes a new miR-335/KDM3A/YAP1 regulation axis, which provided new insight and potential targeting of the metastasized ccRCC.
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http://dx.doi.org/10.1038/s41417-021-00335-3DOI Listing
April 2021

Understanding Breast Implant Illness: Etiology is the Key.

Aesthet Surg J 2021 Apr 19. Epub 2021 Apr 19.

Department for Plastic and Reconstructive Surgery, Institute for Muskulosceletal Medicine, Westfaelische Wilhelms-University Muenster, Muenster, Germany.

As breast augmentation is one of the most popular cosmetic procedure, since their FDA approval in 1962 the safety of implant-related complications has been broadly disputed. Although decades have passed, breast implant illness still represents a poorly defined and controversial complication. With an upcoming nonscientific discussion on mainstream media and social media, revealing BII's etiology is quite urgent, finally influencing patients' decision and creating a trustful doctor-patient relationship. Up to now, little or no scientific research is available on BII and no final conclusion regarding its etiology, clinical manifestations, diagnostic criteria or treatment has been made up to now. This review aims to give an overview of etiology hypothesis of BII and seek inspiration to improve BII patients' conditions.
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http://dx.doi.org/10.1093/asj/sjab197DOI Listing
April 2021

Red and processed meat consumption and cancer outcomes: Umbrella review.

Food Chem 2021 Mar 27;356:129697. Epub 2021 Mar 27.

Department of Urology/Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

The purpose of this umbrella review was to evaluate the quality of evidence, validity and biases of the associations between red and processed meat consumption and multiple cancer outcomes according to existing systematic reviews and meta-analyses. The umbrella review identified 72 meta-analyses with 20 unique outcomes for red meat and 19 unique outcomes for processed meat. Red meat consumption was associated with increased risk of overall cancer mortality, non-Hodgkin lymphoma (NHL), bladder, breast, colorectal, endometrial, esophageal, gastric, lung and nasopharyngeal cancer. Processed meat consumption might increase the risk of overall cancer mortality, NHL, bladder, breast, colorectal, esophageal, gastric, nasopharyngeal, oral cavity and oropharynx and prostate cancer. Dose-response analyses revealed that 100 g/d increment of red meat and 50 g/d increment of processed meat consumption were associated with 11%-51% and 8%-72% higher risk of multiple cancer outcomes, respectively, and seemed to be not correlated with any benefit.
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http://dx.doi.org/10.1016/j.foodchem.2021.129697DOI Listing
March 2021

Assessment of the mechanism of drug resistance in Trichophyton mentagrophytes in response to various substances.

BMC Genomics 2021 Apr 7;22(1):250. Epub 2021 Apr 7.

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Background: Trichophyton mentagrophyte (TM), a zoonotic pathogen, has been endangering public health due to emerging drug resistance. Although increased attention is paid to this issue, there is very limited research available on drug resistance in TM. In this study, we studied the gene and proteomic changes, morphological changes, cellular fat localization, fat content changes, and biofilm of TM treated with different substances.

Results: The TM growth curve showed a positive correlation with the concentration of Fenarimol (FE), genistein (GE), clotrimazole (KM), and Miconazole nitrate salt (MK). The morphology of TM cells changed in different degrees after treatment with different substances as observed by TEM and SEM. The results showed that under KM and berberine hydrochloride (BB) treatment, a total of 3305 differentially expressed genes were detected, with the highest number in the KM-treated group (578 up-regulated and 615 down-regulated). A total of 847 proteins and 1850 peptides were identified in TM proteomics. Nile red staining showed that the fat content of TM was significantly higher in the BB-, ethidium bromide- (EB), FE-, KM-, Adriamycin hydrochloride- (YA), and MK-treated group compared to the control group. Results of the biofilm thickness showed that it gradually increased under treatment with specific concentrations of KM or BB, which may be related to the up-regulation of ERG25 and CYP related gene proteins.

Conclusions: It is suggested that in order to effectively deal with dermatomycosis caused by TM, it is necessary to inhibit the expression of ERG25 and CYP related genes and fat metabolism, which can result in the inhibition of the production of biofilm by the fungus and solve the problem of fungal drug resistance in clinical settings.
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http://dx.doi.org/10.1186/s12864-021-07520-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028809PMC
April 2021

Preliminary Assessment of the Resource and Exploitation Potential of Lower Permian Marine-Continent Transitional Facies Shale Gas in the Huainan Basin, Eastern China, Based on a Comprehensive Understanding of Geological Conditions.

ACS Omega 2021 Mar 22;6(12):8502-8516. Epub 2021 Mar 22.

School of Earth and Environment, Anhui University of Science & Technology, Huainan 232001, China.

The Huainan Basin in eastern China contains abundant shale gas resources; the Lower Permian is an exploration horizon with a high potential for shale gas in marine-continent transitional facies. However, few detailed analyses have investigated shale gas in this area. In this paper, a comprehensive investigation of the geochemical characteristics, physical properties, and gas-bearing capacities of shale reservoirs was conducted, and the resource and exploitation potential were evaluated. The results show that the cumulative thicknesses of the Shanxi Formation (P) and lower Shihezi Formation (P) are mostly greater than 35 and 65 m, respectively. The TOC contents of the P and P shale vary from 0.11 to 8.87% and from 0.22 to 14.63%, respectively; the kerogens predominantly belong to type II with minor amounts of type I or type III kerogens; average values range between 0.83 and 0.94% and between 0.82 and 1.02% in P and P, respectively; the shale samples are primarily at a low maturity, while some shale samples have entered the high-maturity stage. The shale reservoirs have low permeability and porosity in P and P, respectively. The pores of the P shale reservoir are characterized by well-developed micropores and transition pores and poorly developed mesopores, while the pores in the P shale reservoir are all characterized by well-developed micropores and transition pores and some well-developed macropores; the different pore types in the shale reservoirs developed in the organic matter, clay minerals, and pyrite, while a few endogenous fractures developed in the organic matter and structural fractures developed in the minerals. The total shale gas contents in P and P are 2.85 and 2.96 m t, respectively. The P shale reservoir has a higher hydrocarbon generation potential than P and has a lower gas generation potential. The total shale gas amounts in P and P are 3602.29-4083.04 × 10 and 2811.04-3450.77 × 10 m, respectively. Further research on shale gas exploration and exploitation for these formations needs to be performed.
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http://dx.doi.org/10.1021/acsomega.1c00290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015131PMC
March 2021

Thalamocortical Functional Connectivity in Patients With White Matter Hyperintensities.

Front Aging Neurosci 2021 18;13:632237. Epub 2021 Mar 18.

Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

White matter hyperintensities (WMH)s is a very common neuroradiological manifestation in the elderly and is an increased risk of dementia and cognitive decline. As we all know, the thalamocortical circuit plays an important part in cognition regulation. However, the role of this circuit in WMHs and its related cognitive deficits is still unclear. Eighty WMH patients and 37 healthy controls (HCs) were enrolled in the current study. WMH patients were divided into a mild WMH group ( = 33) and moderate-severe WMH group ( = 47) according to Fazekas scores. Resting-state functional magnetic resonance imaging (rs-fMRI) data of all participants were collected for thalamocortical functional connectivity (FC) analysis. The analysis was performed in two steps. First, the whole cerebral cortex was divided into six regions of interest (ROIs), which were used as seeds to investigate the changes of FC with the thalamus. Then, the subregion of the thalamus generated in the previous step was used as the seed for FC analysis with the whole brain. In the first step of FC analysis, it was found that precentral gyrus (PrCG)-interthalamic adhesion (ITA) FC values in moderate-severe WMH group were higher than those in HC and mild WMH groups. However, when compared with the HC group, the increase of PrCG-ITA FC values in mild WMH group was not statistically significant. In the second step of FC analysis, the ITA was set as the seed, and compared with the HC group, the results showed that the FC values of the ITA-medial frontal gyrus (MFG) in mild group and moderate-severe WMH groups were significantly increased. In addition, the FC values in moderate-severe group were significantly higher than those in mild group. Finally, it was also found that FC values (PrCG-ITA and ITA-MFG) were significantly correlated with neuropsychological test results for multiple cognitive functions such as memory, execution and attention in WMH patients. Abnormal thalamocortical FC was closely related with cognitive impairments in WMH patients.
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http://dx.doi.org/10.3389/fnagi.2021.632237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012554PMC
March 2021

Research Progress and Challenges in Vaccine Development against Classical Swine Fever Virus.

Viruses 2021 03 10;13(3). Epub 2021 Mar 10.

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Classical swine fever (CSF), caused by CSF virus (CSFV), is one of the most devastating viral epizootic diseases of swine in many countries. To control the disease, highly efficacious and safe live attenuated vaccines have been used for decades. However, the main drawback of these conventional vaccines is the lack of differentiability of infected from vaccinated animals (DIVA concept). Advances in biotechnology and our detailed knowledge of multiple basic science disciplines have facilitated the development of effective and safer DIVA vaccines to control CSF. To date, two types of DIVA vaccines have been developed commercially, including the subunit vaccines based on CSFV envelope glycoprotein E2 and chimeric pestivirus vaccines based on infectious cDNA clones of CSFV or bovine viral diarrhea virus (BVDV). Although inoculation of these vaccines successfully induces solid immunity against CSFV, none of them could ideally meet all demands regarding to safety, efficacy, DIVA potential, and marketability. Due to the limitations of the available choices, researchers are still striving towards the development of more advanced DIVA vaccines against CSF. This review summarizes the present status of candidate CSFV vaccines that have been developed. The strategies and approaches revealed here may also be helpful for the development of new-generation vaccines against other diseases.
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http://dx.doi.org/10.3390/v13030445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998128PMC
March 2021

Large-scale molecular epidemiological analysis of AAV in a cancer patient population.

Oncogene 2021 Apr 29;40(17):3060-3071. Epub 2021 Mar 29.

Horae Gene Therapy Center, University of Massachusetts, Medical School, Worcester, MA, USA.

Recombinant adeno-associated viruses (rAAVs) are well-established vectors for delivering therapeutic genes. However, previous reports have suggested that wild-type AAV is linked to hepatocellular carcinoma, raising concern with the safety of rAAVs. In addition, a recent long-term follow-up study in canines, which received rAAVs for factor VIII gene therapy, demonstrated vector integration into the genome of liver cells, reviving the uncertainty between AAV and cancer. To further explore this relationship, we performed large-scale molecular epidemiology of AAV in resected tumor samples and non-lesion tissues collected from 413 patients, reflecting nine carcinoma types: breast carcinoma, rectal cancer, pancreas carcinoma, brain tumor, hepatoid adenocarcinoma, hepatocellular carcinoma, gastric carcinoma, lung squamous, and adenocarcinoma. We found that over 80% of patients were AAV-positive among all nine types of carcinoma examined. Importantly, the AAV sequences detected in patient-matched tumor and adjacent non-lesion tissues showed no significant difference in incidence, abundance, and variation. In addition, no specific AAV sequences predominated in tumor samples. Our data shows that AAV genomes are equally abundant in tumors and adjacent normal tissues, but lack clonality. The finding critically adds to the epidemiological profile of AAV in humans, and provides insights that may assist rAAV-based clinical studies and gene therapy strategies.
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http://dx.doi.org/10.1038/s41388-021-01725-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087635PMC
April 2021

Effects of aboveground and belowground litter inputs on the balance of soil new and old organic carbon under the typical forests in subtropical region.

Ying Yong Sheng Tai Xue Bao 2021 Mar;32(3):825-835

Forest Ecology & Stable Isotope Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Litter is one of the most important factors controlling the accumulation, stabilization, and turnover of soil organic carbon (SOC) in forests. There is a knowledge gap of the impacts of aboveground and belowground litter inputs on the balance of new and old SOC under different forests in subtropical region. We examined the effects of aboveground and belowground litter inputs on SOC turnover using isotopic tracing technique, based on a 3-year C3 plants/C4 soil replacement experiment in natural forest (NF), Masson pine () plantation (PM) and Chinese fir () plantation (CL). Our results showed that forest types, litter treatments, and sampling time significantly affected SOC contents, δC, new and old SOC contents. Moreover, there were significant interactions between forest types and litter treatments. Litter input increased SOC content and net SOC increment, with higher sensitivity of NF than CL. Litter inputs decreased soil δC, with lower values in NF and PM compared to CL. For PM, the new SOC content in belowground litter treatment was significantly higher than that in aboveground litter treatment. The contents of old SOC were lower in belowground litter treatment than aboveground litter treatment in the NF and CL. Above- and below-ground biomass were positively correlated with SOC content and net increment. Belowground litter biomass were positively correlated with soil C/N ratio and new SOC content. Our results implied that belowground litter input had stronger effects on SOC turnover compared to aboveground litter input, with the effects varying among different forests. Our results provided new information on SOC accumulation and on sustainable management of the typical forests in subtropical region.
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http://dx.doi.org/10.13287/j.1001-9332.202103.008DOI Listing
March 2021

[Relationships Between Microplastic and Surrounding Soil in an E-Waste Zone of China].

Huan Jing Ke Xue 2021 Mar;42(3):1073-1080

School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.

Microplastic pollution is ubiquitous and has attracted significant public attention. Recent research on microplastic has focused on aquatic environments, but its impacts on soil ecosystems remain poorly understood, especially in e-waste dismantling zones. The objective of this study was to investigate the relationships between microplastic and surrounding soil in abandoned e-waste disassembling plots with different dismantling methods focusing on ecotoxicology and microbiology in Guiyu, Shantou District, Guangdong Province. The surface morphology of collected microplastics showed signs of aging and degradation, possibly due to their long-term exposure in the soil and the original disassembling methods. In addition, there were diverse metal elements at different surface positions of the same microplastic sample based on SEM-EDS analyses, indicating that some metal elements carried by microplastics are derived from the surrounding soil rather than being inherent to the microplastic. Moreover, seven heavy metals (Pb, Cd, Cr, As, Ba, Co, and Ni) inherent in microplastic were identified using ICP-OES, revealing that the concentrations varied in different sampling plots were typically higher than in the surrounding soil. In particular, the concentration of Ba in microplastic was 10 orders of magnitude higher than in soil. Indeed, Ba in the form of BaSO is widely used as a filler in numerous plastics. Furthermore, microplastic-associated microorganisms were examined using 16S rRNA sequencing, and the relationships between the top 50 genera of microplastic-bound bacteria and soil environmental factors were analyzed using the Spearman's rank correlation coefficient. Microorganisms primarily originated from the surroundings of microplastics; therefore, environmental factors could directly affect the microbial communities associated with this type of pollutant. Importantly, different dismantling methods were associated with distinct soil environmental factors, and their correlations with microplastic-associated microorganisms also varied.
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http://dx.doi.org/10.13227/j.hjkx.202007245DOI Listing
March 2021

The noncoding RNAs SNORD50A and SNORD50B-mediated TRIM21-GMPS interaction promotes the growth of p53 wild-type breast cancers by degrading p53.

Cell Death Differ 2021 Mar 19. Epub 2021 Mar 19.

Key Laboratory for Tumor Precision Medicine of Shaanxi Province and Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, PR China.

Small nucleolar RNA SNORD50A and SNORD50B (SNORD50A/B) has been reported to be recurrently deleted and function as a putative tumor suppressor in different types of cancer by binding to and suppressing the activity of the KRAS oncoproteins. Its deletion correlates with poorer patient survival. However, in this study, we surprisingly found that SNORD50A/B loss predicted a better survival in breast cancer patients carrying wild-type p53. Functional studies showed that SNORD50A/B deletion strongly inhibited the proliferation, migration, invasion and tumorigenic potential, and induced cell cycle arrest and apoptosis in p53 wild-type breast cancer cells, while exerted the opposite effects in p53 mutated breast cancer cells. This was also supported by ectopically expressing SNORD50A/B in both p53 wild-type and mutated breast cancer cells. Mechanistically, SNORD50A/B clearly enhances the interaction between E3 ubiquitin ligase TRIM21 and its substrate GMPS by forming a complex among them, thereby promoting GMPS ubiquitination and its subsequent cytoplasmic sequestration. SNORD50A/B deletion in p53 wild-type breast cancer cells will release GMPS and induce the translocation of GMPS into the nucleus, where GMPS can recruit USP7 and form a complex with p53, thereby decreasing p53 ubiquitination, stabilizing p53 proteins, and inhibiting malignant phenotypes of cancer cells. Altogether, the present study first reports that SNORD50A/B plays an oncogenic role in p53 wild-type breast cancers by mediating TRIM21-GMPS interaction.
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http://dx.doi.org/10.1038/s41418-021-00762-7DOI Listing
March 2021

SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice.

Animal Model Exp Med 2021 03 6;4(1):2-15. Epub 2021 Mar 6.

Key Laboratory of Human Disease Comparative Medicine National Health Commission of China (NHC) Institute of Laboratory Animal Science Peking Union Medicine College Chinese Academy of Medical Sciences Beijing China.

Background: Cardiovascular diseases (CVDs) and diabetes mellitus (DM) are top two chronic comorbidities that increase the severity and mortality of COVID-19. However, how SARS-CoV-2 alters the progression of chronic diseases remain unclear.

Methods: We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice. SARS-CoV-2's impacts on pathogenesis of chronic diseases were studied through histopathological, virologic and molecular biology analysis.

Results: Pre-existing CVDs resulted in viral invasion, ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection. Viral infection increased fasting blood glucose and reduced insulin response in DM model. Bone mineral density decreased shortly after infection, which associated with impaired PI3K/AKT/mTOR signaling.

Conclusion: We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases. Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection, which further aggravated the pre-existing diseases. This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection.
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http://dx.doi.org/10.1002/ame2.12155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954823PMC
March 2021

Intramedullary nails in combination with reconstruction plate in the treatment of unstable intertrochanteric femoral fractures with lateral wall damage.

Int Orthop 2021 Mar 17. Epub 2021 Mar 17.

Department of Trauma Orthopedic, The Naval Medical University, Shanghai, 200433, China.

Background: The unstable intertrochanteric femur fracture remains a challenge for surgeons. However, few studies have compared the clinical effectiveness of intramedullary nail in combination with a reconstruction plate and intramedullary nail alone in the treatment of patients with unstable intertrochanteric femoral fractures with lateral wall damage.

Methods: This study retrospectively analyzed 16 patients with 31 A3 intertrochanteric fractures treated with the intramedullary nail in combination with reconstruction plate (the study group) and 19 patients with 31 A3 intertrochanteric fractures treated with intramedullary nail alone (the control group) between January 2012 and January 2018. The operation time, intra-operative blood loss, time of fracture healing, and complication rates of post-operative fixation failure were assessed between the two groups. At the follow-up of post-operative six and 12 months, Harris hip score (HHS) and the Parker-Palmer mobility score (PPMS) were used to evaluate the functional states and mobility levels.

Results: The distribution of all basic characteristics was similar between the two groups (P ˃ 0.05). The study group had longer operation time and more intra-operative blood loss in comparison with the control group (P < 0.001), while the study group had shorter fracture healing time (P = 0.03) and lower fixation failure rate as compared with the control group. Regarding the functional outcome, the study group had higher HHSs and PPMS than the control group (P = 0.003).

Conclusions: Although intramedullary nails in combination with reconstruction plates had longer operation time and more intra-operative blood loss, it might be superior to intramedullary nail alone in terms of fracture healing time, fixation failure complication rate, and post-operative functional recovery.
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http://dx.doi.org/10.1007/s00264-021-05004-6DOI Listing
March 2021

Clinical Characteristics and Prognostic Factors of Testicular Sarcoma: A Population-Based Study.

Front Oncol 2021 25;11:614093. Epub 2021 Feb 25.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Objectives: To study clinical characteristics and factors that may affect the prognosis of testicular sarcoma patients.

Patients And Methods: In the Surveillance Epidemiology and End Results database (2006-2016), people with testicular sarcoma were enrolled in our research. Multivariable Cox proportional hazard model and Multivariable Logistic regression model were used to compare the impact of different factors on cancer-specific survival, localized metastasis, and distant metastasis.

Results: This research was based on the registry information of 158 testicular sarcoma patients. All patients with a median age of 17.00 (1.00-93.00) years were pathologically diagnosed with orchiectomy or needle biopsy specimens. Patients with Grade I, II, III, and IV testicular sarcoma accounted for 34.29% (n = 24), 10.10% (n = 7), 22.86% (n = 16), and 32.86% (n = 23) of all patients, respectively. There were 42 (30.43%), 53 (38.41%), 15 (10.87%), 20 (14.49%), 5 (3.62%), 3 (2.17%) patients with Tis, T1, T2, T3, T4, and >T4 (the invasion degree exceeded the staging system of testicular cancer) disease respectively. Among all included patients, localized metastasis occurred in 31 (20.13%) patients, distant metastasis was found in 28 (18.18%) patients during observation, and 61.69% (n = 95) had no metastasis. Thirty-two (20.25%) patients died of this cancer. According to our study, patients with distant metastasis [OR = 17.86, 95% CI (4.63-68.84), p < 0.0001] and T3 disease [OR = 4.13, 95% CI (1.10-15.53), p = 0.0359] were more likely to die of this cancer. Patients with advanced T stage were more likely to occur distant metastasis, [OR = 13.91, 95% CI (1.80-107.54), p = 0.0116] for T3 and [OR = 16.36, 95% CI (1.36-196.21), p = 0.0275] for T4.

Conclusions: According to our research, factors including metastasis and higher T stage were significantly related with poorer prognosis of testicular sarcoma. Higher T stage was also found to be a risk factor of distant metastasis. The recognization of these poor prognostic factors may allow physicians to make comprehensive and appropriate management decision for testicular sarcoma patients.
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http://dx.doi.org/10.3389/fonc.2021.614093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959772PMC
February 2021

Giant bilateral primary scrotal lipoma along with lipomas in multiple sites of the body: a case report and literature review.

Transl Androl Urol 2021 Feb;10(2):983-990

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Mesenchymal neoplasms of the scrotum are extremely rare in the clinical practices, in which lipoma is the most common benign neoplasm of them. Primary scrotal lipoma refers to the lipoma originated from scrotal wall. We reported a rare case of a 47-year-old man who suffered from bilateral giant primary scrotal lipoma along with lipomas in multiple sites of the body. Scrotal ultrasound indicated that huge hypoechogenic masses were observed in the bilateral scrotums. Contrast-enhanced computed tomography (CT) indicated increased fat density in the scrotal areas. Surgical resection was performed on both scrotal neoplasms. Diagnosis of lipoma was confirmed by the pathological examination through the morphological observation as well as the absence of murine double minute2 (MDM2) gene amplification in the fluorescence in situ hybridization (FISH) test. After five months of follow-up, the wound of the patient recovered well and no sign of local recurrence was observed. Based on the literature review, very few cases of primary scrotal lipoma were reported in the scientific literature up to date, and this is the first report of bilateral primary scrotal lipoma along with multiple lipomas of the body. We presented this case as a rare phenomenon. Although primary scrotal lipoma is rare, clinicians should take it into account when encountering similar scrotal lesions and know the methods for diagnosis and how to make differential diagnosis with other diseases, which is associated with the patient's treatment strategy and prognosis.
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http://dx.doi.org/10.21037/tau-20-1073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947464PMC
February 2021

Application of DNP-enhanced solid-state NMR to studies of amyloid-β peptide interaction with lipid membranes.

Chem Phys Lipids 2021 May 11;236:105071. Epub 2021 Mar 11.

School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. Electronic address:

The cellular membrane disruption induced by the aggregation of Aβ peptide has been proposed as a plausible cause of neuronal cell death during Alzheimer's disease. The molecular-level details of the Aβ interaction with cellular membranes were previously probed using solid state NMR (ssNMR), however, due to the limited sensitivity of the latter, studies were limited to samples with high Aβ-to-lipid ratio. The dynamic nuclear polarization (DNP) is a technique for increasing the sensitivity of NMR. In this work we demonstrate the feasibility of DNP-enhanced ssNMR studies of Aβ peptide interacting with various model liposomes: (1) a mixture of zwitterionic 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and negatively charged 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG); (2) a mixture of POPC, POPG, cholesterol, sphingomyelin and ganglioside GM1; (3) the synaptic plasma membrane vesicles (SPMVs) extracted from rat brain tissues. In addition, DNP-ssNMR was applied to capturing changes in Aβ conformation taking place upon the peptide insertion into POPG liposomes. The signal enhancements under conditions of DNP allow carrying out informative 2D ssNMR experiments with about 0.25 mg of Aβ peptides (i.e. reaching Aβ-to-lipid ratio of 1:200). In the studied liposome models, the C NMR chemical shifts at many C-labelled sites of Aβ are characteristic of β-sheets. In addition, in POPG liposomes the peptide forms hydrophobic contacts F19-L34 and F19-I32. Both the chemical shifts and hydrophobic contacts of Aβ in POPG remain the same before and after 8 h of incubation. This suggests that conformation at the C-labelled sites of the peptide is similar before and after the insertion process. Overall, our results demonstrate that DNP helps to overcome the sensitivity limitation of ssNMR, and thereby expand the applicability of ssNMR for charactering the Aβ peptide interacting with lipids.
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http://dx.doi.org/10.1016/j.chemphyslip.2021.105071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022895PMC
May 2021

Vinculin orchestrates prostate cancer progression by regulating tumor cell invasion, migration, and proliferation.

Prostate 2021 May 12;81(6):347-356. Epub 2021 Mar 12.

Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Background: Prostate cancer (PCa) is a leading cause of death in men, and effective treatment of PCa requires further development. Our study aimed to investigate the potential role of vinculin (VCL) in PCa progression in vitro and in vivo.

Methods: We investigated the methylation level of the VCL promoter based on the TCGA database. The knockdown efficacy of VCL gene expression was confirmed by quantitative polymerase chain reaction, Western blot analysis, and immunofluorescence. Furthermore, morphological changes in PCa cells were detected using phalloidin staining. The mobility of PCa cells was measured using transwell assays and high-content analysis. Moreover, cell growth and viability were determined using the colony formation and cell counting kit-8 assays. The role of VCL in tumor growth in vivo was investigated using a subcutaneous xenograft model generated by injecting tumor cells into the right flank of BALB/c nude mice.

Results: The methylation level of the VCL promoter in PCa was significantly downregulated concomitant with age and the progression of nodal metastasis. VCL expression was markedly decreased by shRNA. Importantly, VCL knockdown significantly changed the cell morphology; inhibited the migration, invasion, and movement; and repressed colony formation and viability of PCa cells in vitro. Furthermore, downregulation of VCL suppressed tumor growth in vivo.

Conclusions: Our study comprehensively evaluated the role of VCL in PCa progression in vivo and in vitro. The findings of the present study suggest that VCL can be a potential target for PCa prognosis and treatment.
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http://dx.doi.org/10.1002/pros.24113DOI Listing
May 2021

N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells.

J Immunother Cancer 2021 Mar;9(3)

Department of Urology, West China Hospital of Sichuan University, Chengdu, China

Background: Few patients with prostate cancer benefit from current immunotherapies. Therefore, we aimed to explore new strategies to change this paradigm.

Methods: Human tissues, cell lines and in vivo experiments were used to determine whether and how N-cadherin impacts the production of programmed death ligand-1 (PD-L1) and indole amine 2,3-dioxygenase (IDO-1) and whether N-cadherin can increase the production of effector (e)Treg cells. Then, we used PC3-bearing humanized non-obese diabetic/severe combined immunodeficiency IL2Rγnull (hNSG) mice with an intravenous injection of human CD34+ hematopoietic stem cells into the tail vein to evaluate whether the N-cadherin antagonist N-Ac-CHAVC-NH2 (designated ADH-1) could improve the therapeutic effect of tumor-infiltrating lymphocyte (TIL)-related treatment.

Results: N-cadherin dramatically upregulated the expression of PD-L1 and IDO-1 through IFN-γ (interferongamma) signaling and increasing the production of free fatty acids that could promote the generation of eTreg cells. In preclinical experiments, immune reconstitution mediated by TILs slowed tumor growth and extended the survival time; however, this effect disappeared after immune system suppression by PD-L1, IDO-1 and eTreg cells. Furthermore, ADH-1 effectively reduced immunosuppression and enhanced TIL-related therapy.

Conclusions: These data show that the N-cadherin antagonist ADH-1 promotes TIL antitumor responses. This important hurdle must be overcome for tumors to respond to immunotherapy.
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http://dx.doi.org/10.1136/jitc-2020-002138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949480PMC
March 2021

Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.).

Sci Rep 2021 Mar 8;11(1):5441. Epub 2021 Mar 8.

Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Nanjing, 210037, China.

Transcription factors (TFs) play crucial regulatory roles in controlling the expression of the target genes in plants. APETALA2/Ethylene-responsive factors (AP2/ERF) are part of a large superfamily of plant-specific TFs whose members are involved in the control of plant metabolism, development and responses to various biotic and abiotic stresses. However, the AP2/ERF superfamily has not been identified systematically in Masson pine (Pinus massoniana), which is one of the most important conifer in southern China. Therefore, we performed systematic identification of the AP2/ERF superfamily using transcriptome sequencing data from Masson pine. In the current study, we obtained 88 members of the AP2/ERF superfamily. All PmAP2/ERF members could be classified into 3 main families, AP2 (7 members), RAV (7 members), ERF (73 members) families, and a soloist protein. Subcellular localization assays suggested that two members of PmAP2/ERF were nuclear proteins. Based on pine wood nematode (PWN) inoculated transcriptome and qPCR analysis, we found that many members of PmAP2/ERF could respond to PWN inoculation and PWN related treatment conditions in vitro. In general, members of the AP2/ERF superfamily play an important role in the response of Masson pine responds to PWN. Furthermore, the roles of the AP2/ERF superfamily in other physiological activities of Masson pine remain to be further studied.
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http://dx.doi.org/10.1038/s41598-021-84855-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940494PMC
March 2021

Whole-genome resequencing reveals genetic characteristics of different duck breeds from the Guangxi region in China.

G3 (Bethesda) 2021 Apr;11(4)

Guangxi Key Laboratory of Livestock Genetic Improvement, Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530001, China.

Distinctive indigenous duck (Anas platyrhynchos) populations of Guangxi, China, evolved due to the geographical, cultural, and environmental variability of this region. To investigate the genetic diversity and population structure of the indigenous ducks of Guangxi, 78 individuals from eight populations were collected and sequenced by whole-genome resequencing with an average depth of ∼9.40×. The eight indigenous duck populations included four breeds and four resource populations. Moreover, the genome data of 47 individuals from two typical meat-type breeds and two native egg-type breeds were obtained from a public database. Calculation of heterozygosity, nucleotide diversity (π), Tajima's D, and FST indicated that the Guangxi populations were characterized by higher genetic diversity and lower differentiation than meat-type breeds. The highest diversity was observed in the Xilin-Ma ducks. Principal component, structure, and phylogenetic tree analyses revealed the relationship between the indigenous duck populations of Guangxi. A mild degree of differentiation was observed among the Guangxi populations, although three populations were closer to the meat or egg breeds. Indigenous populations are famous for their special flavor, small body size, and slow growth rates. Selective sweep analysis revealed the candidate genes and pathways associated with these growth traits. Our findings provide a valuable source of information regarding genetic diversity, population conservation, and genome-associated breeding of ducks.
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http://dx.doi.org/10.1093/g3journal/jkab054DOI Listing
April 2021

Combined Treatment of Radiotherapy and Immunotherapy for Urological Malignancies: Current Evidence and Clinical Considerations.

Cancer Manag Res 2021 24;13:1719-1731. Epub 2021 Feb 24.

Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.

Although it has always been believed that radiation has immunosuppressive effects, more and more preclinical and clinical trials have shown that the combination of radiotherapy and immunotherapy has a potential synergistic effect to treat cancers including urological malignancies. When radiotherapy is combined with immunotherapy, improved prognosis has been observed in different urinary tumors. However, there is no standard treatment, such as the optimal dose/fractionation and the sequence of immunotherapy and radiotherapy. In this review, we discussed the effects of radiotherapy on the cancer immune system and emphasized the synergy of radiotherapy combined with immunotherapy. Although it has significantly improved the prognosis of tumors, there are still some unresolved questions about how to best use this combination in clinical practice. Ongoing trials will provide further information on the interaction of radiotherapy combined with immunotherapy, and are expected to guide clinical practice and improve clinical outcomes.
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http://dx.doi.org/10.2147/CMAR.S288337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917304PMC
February 2021

Simple-Structured Blue Thermally Activated Delayed Fluorescence Emitter for Solution-Processed Organic Light-Emitting Diodes with External Quantum Efficiency of over 20.

ACS Appl Mater Interfaces 2021 Mar 2;13(10):12305-12312. Epub 2021 Mar 2.

Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, P. R. China.

Solution-processed organic light-emitting diodes (OLEDs) are much preferred for the manufacture of low-temperature, low-cost, large-area, and flexible lighting and displaying devices. However, these devices with high external quantum efficiency are still limited, especially for blue ones. In addition, the molecular configurations of emitters are usually complicated, indicative of high costs. In this study, two simple-structured thermally activated delayed fluorescent emitters and its polymer were synthesized with acridine as a donor and benzophenone as an acceptor. Solution-processed OLEDs were prepared based on and as doped light-emitting layer, and -based doped device could achieve maximum external quantum efficiency of up to 20.6% with blue-light emission.
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http://dx.doi.org/10.1021/acsami.1c00412DOI Listing
March 2021

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells.

J Int Med Res 2021 Feb;49(2):300060521992215

Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Objective: Circular RNAs (circRNAs) are non-coding RNAs with high cancer-specific expression and the potential for regulating tumorigenesis. CircRNA_100395 is expressed at low levels in many cancers and is involved in the regulation of tumor cell proliferation and metastasis. However, its expression and function in prostate cancer remain unclear.

Methods: Endogenous expression levels of circRNA_100395 and microRNA-1228 (miR-1228) in prostate cancer tissue samples and cell lines were detected by quantitative reverse transcription-polymerase chain reaction. Cell proliferation, invasion, and migration, cell cycle distribution, and epithelial-mesenchymal transition (EMT) were analyzed in circRNA_100395-overexpressing prostate cancer cells by Cell Counting Kit-8, flow cytometry, Transwell assay, and western blotting, respectively.

Results: CircRNA_100395 expression was downregulated in cancerous prostate tissues relative to adjacent normal tissues. CircRNA_100395 expression was negatively correlated with tumor size, Gleason score, tumor stage, and lymph node metastasis. Moreover, circRNA_100395 overexpression inhibited cell proliferation, altered cell cycle distribution, reduced cell migration and invasion abilities, and suppressed EMT in prostate cancer cells. Moreover, miR-1228 was a direct downstream target of circRNA_100395, and the anti-tumor ability of circRNA_100395 was significantly reversed by miR-1228.

Conclusion: This study identified circRNA_100395 as an anti-tumor circRNA and a potential therapeutic target for prostate cancer.
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http://dx.doi.org/10.1177/0300060521992215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917968PMC
February 2021

Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and E expressed in baculovirus expression system.

Virol J 2021 02 24;18(1):44. Epub 2021 Feb 24.

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.

Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and E are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and E were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E, or their combination (CSFV-E2 + E). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + E. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the E vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the E alone is not able to induce a protective immune response. Taken together, while the E could not confer protection against CSFV, E2 and E2 + E could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.
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http://dx.doi.org/10.1186/s12985-021-01507-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903030PMC
February 2021