Publications by authors named "Qiang Wang"

3,565 Publications

  • Page 1 of 1

Scale ultrasound-guided radial artery cannulation in infant: A randomized controlled trial.

J Vasc Access 2021 Jun 21:11297298211024053. Epub 2021 Jun 21.

Chongqing Key Laboratory of Pediatrics, Department of Anesthesiology, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: Cannulation of the radial artery can be extremely challenging in infants. Scale ultrasound can provide accurate arterial location and guidance for operators. We hypothesized that scale ultrasound helps increase the initial success rate of radial artery cannulation in this population.

Method: Seventy-six infants aged 0-3 months who needed arterial puncture after general anesthesia were randomly divided into two groups (1:1 ratio): the scale ultrasound group and the traditional ultrasound group. The primary endpoints were the success rate of the first attempt and the total success rate of arterial cannulation. The secondary endpoints were the time during arterial puncture and the incidence of vascular complications.

Results: The success rate of the first attempt and the total success rate of arterial cannulation were 92.1% (35/38) versus 50% (19/38) and 100% (38/38) versus 86.8% (33/38) in the scale ultrasound and traditional ultrasound group ( < 0.005), respectively. The median time to ultrasound location, needle entry into the radial artery, and successful cannulation in the scale ultrasound group were significantly shorter than those in the traditional ultrasound group: 10 (8.0, 17.2) s, 15 (11.7, 20) s, and 65 (53.8, 78.5) s vs 30 (26.5, 43.5) s, 35 (23, 51) s, and 224.5 (123.5, 356) s ( < 0.001), respectively. The incidence of hematoma was higher in the traditional group ( < 0.005).

Conclusions: Scale ultrasound-guided radial arterial cannulation can significantly improved initial success rate and overall success rate, shorten puncture time in infant, compared with that achieved with the use of traditional ultrasound guidance.
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http://dx.doi.org/10.1177/11297298211024053DOI Listing
June 2021

A New Free-Standing Aqueous Zinc-Ion Capacitor Based on MnO-CNTs Cathode and MXene Anode.

Nanomicro Lett 2019 Aug 26;11(1):70. Epub 2019 Aug 26.

School of Sciences, Hubei University of Automotive Technology, No. 167 Checheng West Road, Shiyan, 442002, Hubei Province, People's Republic of China.

Restricted by their energy storage mechanism, current energy storage devices have certain drawbacks, such as low power density for batteries and low energy density for supercapacitors. Fortunately, the nearest ion capacitors, such as lithium-ion and sodium-ion capacitors containing battery-type and capacitor-type electrodes, may allow achieving both high energy and power densities. For the inspiration, a new zinc-ion capacitor (ZIC) has been designed and realized by assembling the free-standing manganese dioxide-carbon nanotubes (MnO-CNTs) battery-type cathode and MXene (TiCT) capacitor-type anode in an aqueous electrolyte. The ZIC can avoid the insecurity issues that frequently occurred in lithium-ion and sodium-ion capacitors in organic electrolytes. As expected, the ZIC in an aqueous liquid electrolyte exhibits excellent electrochemical performance (based on the total weight of cathode and anode), such as a high specific capacitance of 115.1 F g (1 mV s), high energy density of 98.6 Wh kg (77.5 W kg), high power density of 2480.6 W kg (29.7 Wh kg), and high capacitance retention of ~ 83.6% of its initial capacitance (15,000 cycles). Even in an aqueous gel electrolyte, the ZIC also exhibits excellent performance. This work provides an essential strategy for designing next-generation high-performance energy storage devices.
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http://dx.doi.org/10.1007/s40820-019-0301-1DOI Listing
August 2019

Disturbance compensation of a multiaperture imaging system based on a coupling rotating prism using an improved model compensation control.

Appl Opt 2021 Jun;60(16):4798-4806

In this paper, a cascade double-loop control (DLC) combined with modeling compensation methods is proposed to improve the tracking precision of the multiaperture imaging system (MAIS). The application of the flexible thin-wall ring mechanism in the coupling rotating prism (CRP) system causes a series of tracking and pointing challenges. Disturbances such as friction, shaft deformation, and model perturbation significantly deteriorate the tracking and pointing accuracy of the CRP. Two different modeling compensation methods that are interfaced with classical DLC are proposed to guarantee the tracking precision of the MAIS. Moreover, the disturbance observation and compensation performance of two different modeling compensation methods are analyzed and compared. Finally, simulation and experiment results indicate that the proposed control methods, especially model compensation based on speed close-loop control, obtain the best performance for disturbance rejection in the MAIS.
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http://dx.doi.org/10.1364/AO.425300DOI Listing
June 2021

Vortex states in an acoustic Weyl crystal with a topological lattice defect.

Nat Commun 2021 Jun 16;12(1):3654. Epub 2021 Jun 16.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore.

Crystalline materials can host topological lattice defects that are robust against local deformations, and such defects can interact in interesting ways with the topological features of the underlying band structure. We design and implement a three dimensional acoustic Weyl metamaterial hosting robust modes bound to a one-dimensional topological lattice defect. The modes are related to topological features of the bulk bands, and carry nonzero orbital angular momentum locked to the direction of propagation. They span a range of axial wavenumbers defined by the projections of two bulk Weyl points to a one-dimensional subspace, in a manner analogous to the formation of Fermi arc surface states. We use acoustic experiments to probe their dispersion relation, orbital angular momentum locked waveguiding, and ability to emit acoustic vortices into free space. These results point to new possibilities for creating and exploiting topological modes in three-dimensional structures through the interplay between band topology in momentum space and topological lattice defects in real space.
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http://dx.doi.org/10.1038/s41467-021-23963-7DOI Listing
June 2021

Divergent camptothecin biosynthetic pathway in Ophiorrhiza pumila.

BMC Biol 2021 Jun 16;19(1):122. Epub 2021 Jun 16.

Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China.

Background: The anticancer drug camptothecin (CPT), first isolated from Camptotheca acuminata, was subsequently discovered in unrelated plants, including Ophiorrhiza pumila. Unlike known monoterpene indole alkaloids, CPT in C. acuminata is biosynthesized via the key intermediate strictosidinic acid, but how O. pumila synthesizes CPT has not been determined.

Results: In this study, we used nontargeted metabolite profiling to show that 3α-(S)-strictosidine and 3-(S), 21-(S)-strictosidinic acid coexist in O. pumila. After identifying the enzymes OpLAMT, OpSLS, and OpSTR as participants in CPT biosynthesis, we compared these enzymes to their homologues from two other representative CPT-producing plants, C. acuminata and Nothapodytes nimmoniana, to elucidate their phylogenetic relationship. Finally, using labelled intermediates to resolve the CPT biosynthesis pathway in O. pumila, we showed that 3α-(S)-strictosidine, not 3-(S), 21-(S)-strictosidinic acid, is the exclusive intermediate in CPT biosynthesis.

Conclusions: In our study, we found that O. pumila, another representative CPT-producing plant, exhibits metabolite diversity in its central intermediates consisting of both 3-(S), 21-(S)-strictosidinic acid and 3α-(S)-strictosidine and utilizes 3α-(S)-strictosidine as the exclusive intermediate in the CPT biosynthetic pathway, which differs from C. acuminata. Our results show that enzymes likely to be involved in CPT biosynthesis in O. pumila, C. acuminata, and N. nimmoniana have evolved divergently. Overall, our new data regarding CPT biosynthesis in O. pumila suggest evolutionary divergence in CPT-producing plants. These results shed new light on CPT biosynthesis and pave the way towards its industrial production through enzymatic or metabolic engineering approaches.
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http://dx.doi.org/10.1186/s12915-021-01051-yDOI Listing
June 2021

Bioactive sesquiterpenes from Inula helenium.

Bioorg Chem 2021 Jun 6;114:105066. Epub 2021 Jun 6.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China. Electronic address:

Twenty-one eudesmane-type sesquiterpenes, including five new compounds, were isolated from the roots of Inula helenium. The structures of the new compounds (1-5) were determined by extensive spectroscopic data interpretation, single-crystal X-ray diffraction analysis and ECD calculations. Six compounds can synergistically enhance cisplatin effect against ovarian cancer cells, the structure - activity relationship for the synergistic effect of these compounds with cisplatin was revealed for the first time, which provides useful clues to develop novel sensitizers to overcome drug resistance in cancer. In addition, fifteen sesquiterpenes exhibited significant anti-inflammatory activity, which provided promising candidates for development of anti-inflammatory agent.
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http://dx.doi.org/10.1016/j.bioorg.2021.105066DOI Listing
June 2021

Protective Effect of Hydroxysafflor Yellow A against Chronic Mild Stress-induced Memory Impairments by Suppressing Tau Phosphorylation in Mice.

Curr Med Sci 2021 Jun 15. Epub 2021 Jun 15.

Institute of Pathophysiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.

Chronic stress plays a critical role in the etiology of sporadic Alzheimer's disease (AD). However, there are currently no effective drugs that can target chronic stress to prevent AD. In this study, we explored the neuroprotective effect of hydroxysafflor yellow A (HSYA) against chronic mild stress (CMS)-induced memory impairments in mice and the underlying mechanism. The Morris water maze test showed that HSYA significantly reduced CMS-induced learning and memory impairments in mice. HSYA increased the expression of brain-derived neurotrophic factor (BDNF) and activated downstream tropomyosin-related kinase B (TrkB) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B(Akt)/mammalian target of rapamycin (mTOR) signaling. HSYA decreased the expression of regulator of calcineurin 1-1L (RCAN1-1L) that could promote the activity of glycogen synthase kinase-3β (GSK-3β). HSYA also attenuated tau phosphorylation by inhibiting the activity of GSK-3β and cyclin-dependent kinase-5 (Cdk5). Our data indicated that HSYA has protective effects against CMS-induced BDNF downregulation, tau phosphorylation and memory impairments. HSYA may be a promising therapeutic candidate for AD by targeting chronic stress.
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http://dx.doi.org/10.1007/s11596-021-2369-3DOI Listing
June 2021

Superhydrophobic Polymer Topography Design Assisted by Machine Learning Algorithms.

ACS Appl Mater Interfaces 2021 Jun 15. Epub 2021 Jun 15.

Digital Manufacturing and Design (DManD), Engineering Product Development Pillar, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore.

Superhydrophobic surfaces have been largely achieved through various surface topographies. Both empirical and numerical simulations have been reported to help understand and design superhydrophobic surfaces. Many such successful surfaces have also been achieved using bioinspired and biomimetic designs. Despite this, identifying the right surface texture to meet the requirements of specific applications is not a straightforward task. Here, we report a hybrid approach that includes experimental methods, numerical simulations, and machine learning (ML) algorithms to create design maps for superhydrophobic polymer topographies. Two design objectives to investigate superhydrophobic properties were the maximum water contact angle (WCA) and Laplace pressure. The design parameters were the geometries of an isotropic pillar structure in micrometer and sub-micrometer length scales. The finite element method (FEM) was validated by the experimental data and employed to generate a labeled dataset for ML training. Artificial neural network (ANN) models were then trained on the labeled database for the topographic parameters (width , height , and pitch ) with the corresponding WCA and Laplace pressure. The ANN models yielded a series of nonlinear relationships between the topographic design parameters and the WCA and Laplace pressure and substantial differences between the micrometer and sub-micrometer length scales. Design maps that span the topography design parameters provide optimal design or tradeoff parameters. This research demonstrates the potential of ANN as a rapid design tool for surface topography exploration.
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http://dx.doi.org/10.1021/acsami.1c04473DOI Listing
June 2021

Guadipyr, a new insecticide, induces microbiota dysbiosis and immune disorders in the midgut of silkworms (Bombyx mori).

Environ Pollut 2021 Jun 5;286:117531. Epub 2021 Jun 5.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China. Electronic address:

Guadipyr, which combines neonicotinoid and semicarbazone functional groups in one molecule, exhibits good activity on several pests and high acute and chronic toxicity to silkworms (Bombyx mori). In this report, the effects of low-dose guadipyr on the midgut microbiota and immune system of silkworms were studied. Results showed that the structure and richness of the midgut microbiota of silkworms were altered after being treated with 5.25 mg/L (1/10 of LC) of guadipyr. The abundance of Pseudomonas was evidently increased, whereas Curvibacter was substantially reduced, which might be related to the growth and immunity of silkworms. The expression of key genes in the Toll, IMD, and JAK/STAT pathways, which ultimately led to the downregulation of antimicrobial peptide genes (AMPs), such as CecA, Defensin1, Leb, and glv2, was reduced upon guadipyr exposure. Simultaneously, the suppression of steroid hormone 20-hydroxyecdysone receptor and response genes, such as BR-C Z4, was detected in the exposed groups. The decreased expression of these immune regulatory pathway-related and 20-hydroxyecdysone signal pathway-related genes indicated that the immune system of silkworms was affected by low-dose guadipyr. Our results revealed the negative effects of guadipyr on silkworms and highlighted the unneglectable toxicity of low-dose guadipyr to this economic insect. Given the risk, it is necessary to control the application of guadipyr in or around the mulberry fields.
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http://dx.doi.org/10.1016/j.envpol.2021.117531DOI Listing
June 2021

Evolution of pure hydrocarbon hosts: simpler structure, higher performance and universal application in RGB phosphorescent organic light-emitting diodes.

Chem Sci 2020 May 5;11(19):4887-4894. Epub 2020 May 5.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University Suzhou Jiangsu 215123 China

In the field of phosphorescent organic light-emitting diodes (PhOLEDs), designing high-efficiency universal host materials for red, green and blue (RGB) phosphors has been quite a challenge. To date, most of the high-efficiency universal hosts reported incorporate heteroatoms, which have a crucial role in the device performance. However, the introduction of different kinds of heterocycles increases the design complexity and cost of the target material and also creates potential instability in the device performance. In this work, we show that pure aromatic hydrocarbon hosts designed with the 9,9'-spirobifluorene scaffold are high-efficiency and versatile hosts for PhOLEDs. With external quantum efficiencies of 27.3%, 26.0% and 27.1% for RGB PhOLEDs respectively, this work not only reports the first examples of high-efficiency pure hydrocarbon materials used as hosts in RGB PhOLEDs but also the highest performance reported to date for a universal host (including heteroatom-based hosts). This work shows that the PHC design strategy is promising for the future development of the OLED industry as a high-performance and low-cost option.
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http://dx.doi.org/10.1039/d0sc01238fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159222PMC
May 2020

Risk factors and prevention of postoperative pancreatic fistula after insulinoma enucleation:a retrospective study from a high-volume center.

Pancreatology 2021 Jun 5. Epub 2021 Jun 5.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, 100730, China. Electronic address:

Background/objectives: Enucleation is an effective surgical method to treat pancreatic insulinoma, however, the incidence of clinically relevant postoperative pancreatic fistula (CR-POPF) is high. We aim to investigate the risk factors for CR-POPF which have not been well characterized and develop effective methods to prevent CR-POPF after enucleation.

Methods: This retrospective cohort study included 161 patients diagnosed with insulinoma from June 2016 to July 2020 in Peking Union Medical College Hospital. The risk factors for CR-POPF were evaluated and the role of prophylactic pre-operative pancreatic stent to prevent the occurrence of CR-POPF after enucleation of pancreatic insulinoma were explored.

Results: A cohort of 161 insulinoma cases were reviewed. The CT or MRI imaging reports could be tracked in 108 cases. A total of 96 patients underwent surgery, while 81 experienced pancreatic enucleation. Univariate and multivariate analyses showed that the distance from insulinoma to the main pancreatic duct (MPD) ≤2 mm was an independent risk factor for CR-POPF (p = 0.003, OR = 6.011, 95% Cl 1.852-19.512). The pre-operative pancreatic stent substantially reduced the incidence of CR-POPF in patients with tumor located in proximity to (distance ≤2 mm) the MPD (CR-POPF of the stented group vs the non-stented group: 37.5% vs 71.4%, p = 0.028).

Conclusions: The distance from insulinoma to MPD ≤2 mm is a predictive factor for CR-POPF after enucleation. Pancreatic duct stenting may benefit patients with insulinoma in proximity to the MPD by enabling a lower CR-POPF rate, so it should be considered before the enucleation of the insulinoma in proximity to the MPD (distance ≤2 mm).
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http://dx.doi.org/10.1016/j.pan.2021.06.001DOI Listing
June 2021

The efficacy and safety of tranexamic acid in high tibial osteotomy: a systematic review and meta-analysis.

J Orthop Surg Res 2021 Jun 11;16(1):373. Epub 2021 Jun 11.

Department of Orthopedics, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China.

Objective: The present meta-analysis was conducted to evaluate the efficacy and safety of the application of tranexamic acid (TXA) in patients undergoing high tibial osteotomy (HTO).

Methods: PubMed (MEDLINE), EMBASE, and Cochrane Library were systematically searched for relevant literature from inception until 1 February 2021. A combined searching strategy of subject words and random words was adopted. After testing for potential publication bias and/or heterogeneity, we aggregated variables by using the random-effect model. The primary comparison outcome measures were total blood loss, hemoglobin decrease, drain output, wound complications, thrombotic events, and blood transfusion rate of the TXA group versus control. The meta-analysis was performed using the RevMan 5.3 software.

Results: A total of 5 studies were included involving 532 patients. The results showed that there were significant differences in the two groups concerning total blood loss (95% confidence interval [CI] - 332.74 to - 146.46, P < 0.00001), hemoglobin decrease on postoperative day (POD) 1, 2, and 5 (POD 1 95% CI - 1.34 to - 0.63, P < 0.00001; POD 2 95% CI - 1.07 to - 0.68, P < 0.00001; POD 5 95% CI - 1.46 to - 0.84, P < 0.00001), drain output (POD total 95% CI - 195.86 to - 69.41, P < 0.00001) and wound complications (RR = 0.34, 95% CI 0.12 to 0.97, P = 0.04). Nonsignificant differences were found in the incidence of thromboembolic events (RR = 0.46, 95% CI 0.09 to 2.41, P = 0.36) and blood transfusion rate (RR = 0.25, 95% CI 0.03 to 2.27, P = 0.22).

Conclusions: This meta-analysis of the available evidence demonstrated that TXA could reduce total blood loss, hemoglobin decrease, drain output, and wound complications without increasing the incidence of thromboembolic events in patients undergoing HTO. But there is no obvious evidence that TXA could reduce blood transfusion rates. Further studies, including more large-scale and well-designed randomized controlled trials, are warranted to assess the efficacy and safety issues of routine TXA use in HTO patients.
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http://dx.doi.org/10.1186/s13018-021-02512-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194157PMC
June 2021

Solid-state NMR studies of internuclear correlations for characterizing catalytic materials.

Chem Soc Rev 2021 Jun 11. Epub 2021 Jun 11.

National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China. and University of Chinese Academy of Sciences, Beijing 100049, China.

Understanding the nature of heterogeneous catalysts is critical for the rational design of highly active catalysts, which necessitates in-depth characterization of the structure and properties of catalysts as well as reaction mechanisms. Solid-state NMR correlation spectroscopy is becoming increasingly recognized as a powerful tool in the study of catalysts and catalytic reactions because of its capability to provide atomic-level insights into the structure, interaction and dynamics of molecules by establishing connectivity and proximity between the same or distinct nuclei. This tutorial review focuses on the fundamentals and state-of-the-art applications of solid-state NMR correlation techniques to structural characterization of catalytic materials including zeolites, metal oxides, organometallic complexes and MOFs as well as relevant studies regarding synthesis, synergistic catalysis, host-guest interactions and reaction mechanisms. Various correlation NMR methods that have been employed to address the challenging issues in heterogeneous catalysis are highlighted. This review concludes with outlooks on the promising applications and potential developments of solid-state NMR correlation spectroscopy in catalytic materials.
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http://dx.doi.org/10.1039/d0cs01130dDOI Listing
June 2021

A novel multi-site damage localization method based on near-field signal subspace fitting using uniform linear sensor array.

Ultrasonics 2021 Jun 6;116:106485. Epub 2021 Jun 6.

College of Automation and College of Artificial Intelligence, Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, People's Republic of China.

Damage diagnose imaging methods based on guided waves have been widely developed to depict the position and area of damage in structural health monitoring (SHM). Among them, compact sensor array based imaging methods have been gradually applied with high accuracy, such as phased array imaging, spatial filter imaging, multiple signal classification imaging and so on. However, when the multi-site damage appears nearly, the performance of these methods usually degrades. On one hand, limited to the array aperture, traditional methods cannot distinguish adjacent damage sites. On another hand, some subspace decomposition based methods also fail, suffering from the correlation between scattered array signals from different damage sites. To realize localization of multi-site damage, a novel near-field signal subspace fitting based multi-site damage localization method is proposed in this paper. This method constructs the cost function, which describe the equivalence relation between the signal subspace and array manifold matrix. Then localization of multi-site damage could be realized through multidimensional search for the optimization of cost function. The proposed method is verified on the aluminum panel with 3 damage sites. Experimental results show that the proposed method can realize multi-site damage localization with the angle errors less than 4° and the distance errors less than 35 mm.
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http://dx.doi.org/10.1016/j.ultras.2021.106485DOI Listing
June 2021

Waterjet in Bacterial Clearance of Diabetic Lower Extremity Contaminated Wounds: A Retrospective Cohort Study.

Int J Low Extrem Wounds 2021 Jun 9:15347346211024204. Epub 2021 Jun 9.

92323Zhongshan Hospital, Fudan University, Shanghai, China.

The waterjet debridement is now a standard practice in contaminated or infected diabetic lower extremity wounds. The bacterial clearance of the waterjet debridement remains an important parameter that should be predicted in this application. This study aimed to investigate the waterjet in reducing the diabetic lower extremity wound contaminants. A retrospective cohort study was conducted. Patients' etiology and pathogen diagnosis were established as diabetic lower extremity contaminated wound. The high-power waterjet (Versajet™, Smith-Nephew) was used in the treatment group and conventional surgical methods were used in the control group. The bacteriological swab samples were collected before and after the debridement. The results of bacterial culture were analyzed. A total of 74 patients were included in our study, 40 patients in the treatment group and 34 in the control group. Patient characteristics were well matched. The preoperative bacteriological swab samples of the 2 groups showed no significant difference between each other with a value of .1022. The culture result of postoperative bacteriological swab samples in the treatment group was significantly lower than control with a value of .0099. The odds of bacterial clearance were greater in the treatment group than in the control group (odds ratio, 5.139; 95% confidence interval, 1.386-18.41). As demonstrated by this retrospective research, waterjet debridement reduced the bacterial load in the diabetic lower extremity contaminated wounds.
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http://dx.doi.org/10.1177/15347346211024204DOI Listing
June 2021

[Relationship between IKZF3 Gene Single Nucleotide Polymorphisms and Childhood Acute Lymphoblastic Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):690-695

Department of Hematology,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China,E-mail:

Objective: To investigate the relationship between single nucleotide polymorphisms (SNPs) of IKAROS family Zinc finger 3 (IKZF3) gene and the risk of acute lymphoblastic leukemia (ALL) in children.

Methods: The peripheral blood samples from 286 children with ALL and 382 healthy children were collected and divided into ALL group and control group, respectively. The genotypes of IKZF3 gene at rs62066988 C > T and rs12946510 C > T were detected by quantitative PCR with TaqMan detection system, and their correlation with ALL was analyzed.

Results: The distribution frequencies of CC, CT and TT genotypes at rs62066988 in ALL group were 58.39%, 37.06% and 4.55%, respectively, while those in control group were 69.19%, 27.68% and 3.13%, respectively. The distribution frequencies of CC, CT and TT genotypes at rs12946510 in ALL group were 58.16%, 34.75% and 7.09%, respectively, while those in control group were 55.76%, 37.43% and 6.81%, respectively. Compared with the control group, the distribution frequency of CT/TT genotype at rs62066988 was significantly increased in the ALL group (OR=1.59, 95%CI: 1.16-2.19, P=0.004). However, there was no significant difference in the distribution of rs12946510 C > T polymorphism between ALL group and control group.

Conclusion: The CT/TT genotype of IKZF3 at the site of rs62066988 is associated with the increased risk of ALL in children.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.006DOI Listing
June 2021

A semi-supervised zero-shot image classification method based on soft-target.

Neural Netw 2021 May 25;143:88-96. Epub 2021 May 25.

Center for OPTical IMagery Analysis and Learning, Northwestern Polytechnical University, Xi'an, China. Electronic address:

Zero-shot learning (ZSL) aims at training a classification model with data only from seen categories to recognize data from disjoint unseen categories. Domain shift and generalization capability are two fundamental challenges in ZSL. In this paper, we address them with a novel Soft-Target Semi-supervised Classification (STSC) model. Specifically, an autoencoder network is leveraged, where both labeled seen data from the seen categories and unlabeled ancillary data collected from Internet or other datasets are employed as two branches, respectively. For the branch of labeled seen data, side information are employed as the latent vectors to separately connect the input of encoder and the output of decoder. In this way, visual and side information are implicitly aligned. For the branch of unlabeled ancillary data, it explicitly strengthens the reconstruction ability of the network. Meanwhile, these ancillary data can be viewed as a smooth to the domain distribution, which contributes to the alleviation of the domain shift problem. To further guarantee the generation ability, a Softmax-T loss function is proposed by making full use of the soft target. Extensive experiments on three benchmark datasets show the superiority of the proposed approach under tasks of both traditional zero-shot learning and generalized zero-shot learning.
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http://dx.doi.org/10.1016/j.neunet.2021.05.019DOI Listing
May 2021

Regulatory mechanisms of lipid biosynthesis in microalgae.

Authors:
Hui Chen Qiang Wang

Biol Rev Camb Philos Soc 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.

Microalgal lipids are highly promising feedstocks for biofuel production. Microalgal lipids, especially triacylglycerol, and practical applications of these compounds have received increasing attention in recent years. For the commercial use of microalgal lipids to be feasible, many fundamental biological questions must be addressed based on detailed studies of algal biology, including how lipid biosynthesis occurs and is regulated. Here, we review the current understanding of microalgal lipid biosynthesis, with a focus on the underlying regulatory mechanisms. We also present possible solutions for overcoming various obstacles to understanding the basic biology of microalgal lipid biosynthesis and the practical application of microalgae-based lipids. This review will provide a theoretical reference for both algal researchers and decision makers regarding the future directions of microalgal research, particularly pertaining to microalgal-based lipid biosynthesis.
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http://dx.doi.org/10.1111/brv.12759DOI Listing
June 2021

Thiol/methylthio-functionalized porous aromatic frameworks for simultaneous capture of aromatic pollutants and Hg(II) from water.

J Hazard Mater 2021 Jun 1;418:126244. Epub 2021 Jun 1.

Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, United States.

Simultaneously capturing organic pollutants and heavy metal can greatly reduce the water remediation time and cost, however it is still a great challenge presently. Herein, two novel thiol/methylthio-functionalized porous aromatic frameworks were synthesized as sorbents via the Sonogashira-Hagihara reaction of 1,3,5-triethynylbenzene and 1,3,5-tris(4-bromophenyl) benzene, the subsequent chloromethylation of the phenyl rings, and the final nucleophile substitution of -Cl groups by NaSH/NaSMe. These two sorbents were characterized by FT-IR spectra, energy dispersive X-ray spectra, scanning electron microscope, nitrogen adsorption analysis, thermo-gravimetric analysis, and elemental analyses. Adsorption experiments displayed that new sorbents had high uptake abilities and fast adsorption kinetics for aromatic pollutants and mercury (II) (Hg(II)). The maximum adsorption capacity (Q) of toluene and m-xylene on both new sorbents were 531.9-571.4 mg/g with the kinetic binding rate constants (k) of 0.00276-0.02422 g/mg/min, and the Q values of Hg(II) were 148.1-180.3 mg/g with k of 0.00592-0.01573 g/mg/min. Moreover, new sorbents indicated high simultaneous uptake abilities for these pollutants with good reusability, and finally they were successfully applied to the simultaneous remediation of these pollutants in two simulated sewages with high and low concentration, indicating their great practical application potential in wastewater remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126244DOI Listing
June 2021

Krüppel like factor 10 prevents intervertebral disc degeneration via TGF-β signaling pathway both and .

J Orthop Translat 2021 Jul 15;29:19-29. Epub 2021 May 15.

Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.

Background: Krüppel like factor 10 (KLF10), which is also known as TGF-β Inducible Early Gene-1 (TIEG1), plays a crucial role in regulating cell proliferation, cell apoptosis and inflammatory reaction in human carcinoma cells. Moreover, KLF10 knockout in mice leads to severe defects associated with muscle, skeleton and heart etc. However, the function of KLF10 in intervertebral disc degeneration (IVDD) has not been reported yet.

Methods: The relationship between KLF10 and IVDD were investigated in nucleus pulposus (NP) tissues from human and rats. The role of KLF10 in NP cells was explored via loss or gain of function experiments. IVDD rat models were constructed through needle puncture and the effects of KLF10 in IVDD model of rats were investigated via intradiscal injection of KLF10.

Results: We first found that KLF10 was lowly expressed in degenerative NP tissues and the level of KLF10 showed negative correlation with the disc grades of IVDD patients. Loss or gain of function experiments demonstrated that KLF10 could inhibit apoptosis and enhance migration and proliferation of IL-1β induced NP cells. And KLF10 overexpression reduced extracellular matrix (ECM) degeneration and enhanced ECM synthesis, whereas knockdown of KLF10 resulted in adverse effects. These positive effects of KLF10 could be reversed by the inhibition of TGF-β signaling pathway. , KLF10 overexpression alleviated IVDD.

Conclusions: This is the first study to reveal that KLF10 was dysregulated in IVDD and overexpressed KLF10 could alleviate IVDD by regulating TGF-β signaling pathway both and , which were involved in prohibiting apoptosis, promoting proliferation and migration of NP cells.The translational potential of this article: Overexpression of KLF10 might be an effective therapeutic strategy in the treatment of IVDD.
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http://dx.doi.org/10.1016/j.jot.2021.04.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141503PMC
July 2021

Fluorescent dissolved organic matter facilitates the phytoavailability of copper in the coastal wetlands influenced by artificial topography.

Sci Total Environ 2021 May 26;790:147855. Epub 2021 May 26.

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, PR China; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, PR China. Electronic address:

Dissolved organic matter (DOM) is a crucial driver in ecosystem services and a central part of the carbon transport and biological cycle in land-sea interaction. DOM exhibits characteristic environmental behavior in the coastal zone, but its sustainability is affected by expanding artificial topography (AT) construction. It requires combining analyses on AT-induced response of field fluorescent DOM (fDOM) and its quenching pattern under metal-complexation. Herein, we conducted systemic investigations into the spatiotemporal dynamics of fDOM compositions with further in-lab verification to study its Cu-binding capacity. We detected three humid-like fDOM components sensitive to AT. The total fDOM intensity was positively correlated with low molecular weight organic acid (LMWOA) extractable Cu and the Cu pools in above-ground biomass. The enriched fDOM serves as an ecological engineer by increasing the Cu mobility, confirmed by an in-lab fluorescence titration. The application of LMWOA greatly enhanced the intensity of one fDOM component, elevated its conditional stability constant, and decreased its quenched proportion, implying that LMWOA might extract part of Cu from fDOM complexation. The present work provides an "fDOM-LMWOA pump" explanation to suggest that fDOM is a novel ecological regulator on vegetation growth under the AT-induced matter accumulation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147855DOI Listing
May 2021

TSHZ3 functions as a tumor suppressor by DNA methylation in colorectal cancer.

Clin Res Hepatol Gastroenterol 2021 Jun 2:101725. Epub 2021 Jun 2.

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. Electronic address:

Objective: Teashirt zinc finger homeobox 3 (TSHZ3) is currently reported to be aberrantly expressed in several tumors, but the detailed functions and epigenetic mechanisms of TSHZ3 in colorectal cancer (CRC) remain unclear.

Materials And Methods: In this study, the TSHZ3 expression in 118 CRC and normal adjacent tissues (NATs) was evaluated, and the methylation status of the TSZH3 promoter region in CRC tissues and cell lines was also analyzed.

Results: The results of PCR analysis showed that TSHZ3 was significantly down-regulated in CRC tissues, and patients with low TSHZ3 levels had a poorer 5-year overall survival (OS) rate. Analyzing the promoter sequence (-1000∼0) by MethPrimer, TSHZ3 promoter was found to harbor abundant of CpG islands. The methylation specific PCR (MSP) analysis presented a relatively hypermethylated status of THSZ3 promoter in CRC samples. The data of MSP and bisulfite sequencing PCR (BSP) also confirmed that CpG sites of TSHZ3 promoter were methylated in CRC cells, and the DNA methyltransferase (DNMT) inhibitor 5-aza-2'-deoxycytidine (5-Aza) could effectively restored the TSHZ3 expression in vitro. Functionally, the proliferation, apoptosis and metastasis of CRC cells were regulated by TSZH3 over-expression, and the suppressing effects of TSHZ3 in CRC were also confirmed in a xenograft mouse model.

Conlusions: Our results indicated that promoter methylation was one of the mechanisms contributing to the down-regulation of TSHZ3 in CRC, and TSZH3 might served as a potential tumor suppressor gene in the development and progression of CRC.
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http://dx.doi.org/10.1016/j.clinre.2021.101725DOI Listing
June 2021

Supercapacitive brophene-graphene aerogel as elastic-electrochemical dielectric layer for sensitive pressure sensors.

J Colloid Interface Sci 2021 May 25;601:355-364. Epub 2021 May 25.

Science and Technology Institute, Hubei Key Laboratory of Advanced Textile Materials & Application, Laboratory for Electron Microscopy, Wuhan Textile University, Wuhan 430073, Hubei, People's Republic of China.

A sensitive pressure sensor based on ultralight and superelastic supercapacitive borophene-graphene aerogel as dielectric layer is reported. The borophene-graphene aerogel not only combines large specific surface area of reduced graphene oxide and high conductivity of borophene, but also exhibits rich porous structure. The strong synergy and intercalation between two different two-dimensional materials benefit electron transfer and electrolyte ion diffusion. On the one hand, the aerogel exhibits greater mass specific capacitance of 330 F g than pure graphene aerogel. More importantly, serving as dielectric layer for pressure sensors with a symmetrical structure, the sensor represents ultra-high sensitivity (0.90 KPa) in the pressure range (<3 KPa), ultra-rapid response time (~110 ms), ultra-low detection limit as 8.7 Pa and excellent working stability after 1000 cycles. In practical application, the sensor demonstrates great performance in monitoring human physiological signals, and agricultural applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.116DOI Listing
May 2021

Systems pharmacological study based on UHPLC-Q-Orbitrap-HRMS, network pharmacology and experimental validation to explore the potential mechanisms of Danggui-Shaoyao-San against atherosclerosis.

J Ethnopharmacol 2021 Jun 2;278:114278. Epub 2021 Jun 2.

Department of Traditional Chinese Medicine, People's Hospital of Yangjiang, Yangjiang, 529500, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China; Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, 110032, China. Electronic address:

Ethnopharmacological Relevance: Atherosclerosis (AS) plays an important role in the pathogenesis of cardiovascular and cerebrovascular diseases. Danggui-Shaoyao-San (DSS) is not only a representative Chinese formula to treat gynecological disorder, but also found its use in AS-related diseases. However, the active ingredients and the anti-AS effects are vague yet.

Aim Of The Study: An integrated strategy combined ultrahigh-performance liquid chromatography quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS), network pharmacology and experiments was carried out to investigate the potential materials and pharmacological mechanisms of DSS for AS.

Materials And Methods: First, UHPLC-Q-Orbitrap-HRMS was applied to identify the active compositions of DSS. Then, the putative targets of DSS relevant to AS were predicted from TCMSP and BATMAN, which were further determined through bioinformatic analyses, including protein-protein interactions (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG). Finally, Western blot, qPCR and ELISA were carried out for target validation in human umbilical vein endothelial cells (HUVECs).

Results: A total of 37 active ingredients of DSS, connecting 47 key targets were identified. The functional enrichment showed that DSS may treat AS through regulating a series of signaling pathways which involving inflammatory responses, immune systems and metabolism. The in vitro experiment revealed that DSS ameliorated AS mainly through anti-inflammatory effects, by reducing the levels of vascular cell adhesion molecule-1 (VCAM1), intercellular adhesion molecule-1 (ICAM1), IL-6, TNF-α, cyclooxygenase-2 (Cox-2) and IL-1β. DSS also inhibited the phosphorylation of IκB-α, NF-κB (p65), p38 and JNK in lipopolysaccharide (LPS)-induced HUVEC injury model. Moreover, as the main bioactive compounds of DSS, paeoniflorin (PF), ferulic acid (FA) and pachymic acid (PA) inhibited IL-6 and TNF-α secretion as well as IκB-α, NF-κB (p65), p38 and JNK activation. All these findings were consistent with the predicted targets and pathways.

Conclusion: Collectively, the basic pharmacological effects and relevant mechanisms of DSS in the treatment of AS were revealed. The results suggest that DSS is a potential drug for AS treatment, and PF, FA, PA may be the core compositions contributing to the pharmacological function of this formula.
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http://dx.doi.org/10.1016/j.jep.2021.114278DOI Listing
June 2021

[Effect of electroacupuncture on expression of α-syn in colon and substantia nigra of Parkinson's disease mice].

Zhen Ci Yan Jiu 2021 May;46(5):362-7

College of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; Shaanxi Key Laboratory of Acupuncture and Herbal Medicine, Xianyang 712046, Shaanxi Province; Key Laboratory of Neurobiology (Acupuncture), Xianyang 712046, Shaanxi Province.

Objective: To observe the effect of electroacupuncture(EA) intervention on the behavior, colon and midbrain substantia nigra α-synuclein(α-syn) expression in Parkinson's disease (PD) mice, so as to explore the mechanism of early EA on prevention of PD.

Methods: C57BL/6J mice were randomly divided into blank, model and EA groups, with 8 mice in each group. The PD mouse model was established by subcutaneous injection of rotenone. Mice in the EA group received EA (2 Hz, 1 mA) at "Quchi"(LI11), "Shangjuxu"(ST37) and acupuncture at "Shenting" (GV24)and "Tianshu"(ST25) for 10 min once daily, with 7 d for a course, a total of 8 courses. Behavioral tests of hanging and climbing poles were carried out before, during and after modeling. The expressions of a-syn in substantia nigra and colon were detected by Western blot and immunohistochemistry or immunofluorescence.

Results: After 8 weeks of modeling, the mice in the model group showed symptoms such as listlessness, dim hair color, coldness and curling up. Compared with the blank group, the hanging score of the model group was significantly lower (<0.01), the pole climbing time and the expressions of α-syn protein in colon and substantia nigra were significantly increased (<0.01). Following intervention, mice in the EA group were always in good mental state, with shiny fur and stable body weight. The hanging scores was significantly higher and the pole climbing time and the expressions of α-syn protein in colon and substantia nigra were significantly down-regulated (<0.05, <0.01) in the EA group relevant to the model group.

Conclusion: Early EA intervention can delay the onset of PD induced by rotenone, which may be related to its effect in reducing the expression of α-syn in midbrain substantia nigra and colon.
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http://dx.doi.org/10.13702/j.1000-0607.200672DOI Listing
May 2021

Effects of Substituting Types of Physical Activity on Body Fat Mass and Work Efficiency among Workers.

Int J Environ Res Public Health 2021 May 12;18(10). Epub 2021 May 12.

Faculty of Sports Science, Sendai University, Miyagi 9891693, Japan.

Low levels of physical activity (PA) not only increase healt h risks but also affect employee productivity. Although daily activity is interdependent with work productivity and personal health, few studies have examined how substituting physical activities would affect health and work efficiency. The present study aimed to investigate how substituting sedentary behaviors (SB) with increased PA and increasing the intensity of low-level activities during waking times affects the body fat mass and work efficiency of employees. Data were collected from 224 Japanese employees. SB, light physical activity (LPA), moderate physical activity (MPA), and vigorous physical activity (VPA) were measured using a tri-axial accelerometer, and body fat mass and work efficiency were also assessed. Analyses of the effects of substituting behaviors were based on the methods used in the isotemporal substitution model. Body fat mass decreased after substituting behaviors for 30 min per day: from SB to VPA (β = -4.800, 95% CI = -7.500; -2.100), from LPA to VPA (β = -4.680, 95% CI = -7.350; -1.980), and from MPA to VPA (β = -4.920, 95% CI = -7.680; -2.190). For work efficiency and physical activities, a higher work efficiency score was observed when substituting SB with LPA (β = 0.120, 95% CI = 0.030; 0.240), and a lower work efficiency score was observed when substituting LPA with VPA (β = -0.660, 95% CI = -1.350; -0.030). These results should help achieve greater results in promoting health and increasing work productivity by properly distributing and practicing daily physical activities during work hours.
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http://dx.doi.org/10.3390/ijerph18105101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151033PMC
May 2021

Effect of different CAD/CAM cutting depths on the post-fatigue load-bearing capacity of novel multilayer zirconia restorations.

J Dent 2021 May 29:103709. Epub 2021 May 29.

School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China. Electronic address:

Objectives: To investigate the post-fatigue load-bearing capacity of restorations milled at various cutting depths in a novel strength- and color-gradient multilayer zirconia CAD/CAM disk.

Methods: Identical crowns were divided into 4 groups (n=25 per group): crowns milled at 3 CAD/CAM cutting depths in multilayer zirconia disks as 3 experiment groups and homogeneous zirconia crowns as a control group. The color differences between various cutting depths were measured. In each group, crowns were tilted at 15° and subjected to fatigue loading for various numbers of cycles (0, 10, 2 × 10, 3 × 10 or 4 × 10, n=5 per subgroup). All surviving crowns were subsequently submitted to a static fracture test to determine load-bearing capacity. The data were analyzed by ANOVA and Weibull distribution analysis. Failure modes were observed with SEM.

Results: For a novel multilayer zirconia, a deeper CAD/CAM cutting depth corresponded to a higher load-bearing capacity but a darker color. After 10 cycles of fatigue loading, there was not a significant reduction in fracture load. However, after 4 × 10 cycles, the load-bearing capacity of all crowns decreased significantly by approximately 50%. For multilayer and homogeneous zirconia materials, similar failure mode and Weibull modulus were identified.

Conclusions: Restorations can be milled at various CAD/CAM cutting depths in a novel multilayer zirconia disk depending on the durability and aesthetic requirements. The load-bearing capacity of multilayer restorations degraded significantly after 4 × 10cycles of fatigue loading, but not after 10cycles.

Clinical Significance: For a novel multilayer zirconia disk, a posterior restoration can be milled from the middle or bottom of the disk to improve its load-bearing capacity. For an anterior restoration, to meet the aesthetic requirements, milling the entire thickness of a multilayer disk is recommended.
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http://dx.doi.org/10.1016/j.jdent.2021.103709DOI Listing
May 2021

Effect of Acetylene Links on Electronic and Optical Properties of Semiconducting Graphynes.

ACS Omega 2021 Apr 19;6(16):10997-11004. Epub 2021 Apr 19.

Department of Mechanic Engineering & Engineering Science, National University of Singapore, Singapore 117575, Singapore.

The family of graphynes, novel two-dimensional semiconductors with various and fascinating chemical and physical properties, has attracted great interest from both scientific and industrial communities. Currently, the focus is on graphdiyne or graphyne-2. In this work, we systematically study the effect of acetylene, i.e., carbon-carbon triple bond, links on the electronic and optical properties of a series of graphynes (graphyne-, where = 1-5, the number of acetylene bonds) using ab initio calculations. We find an even-odd pattern, i.e., = 1, 3, 5 and = 2, 4 having different features, which has not been discovered in studying graphyne or graphdiyne alone. It is found that as the number of acetylene bonds increases, the electron effective mass increases continuously in the low-energy range because of the flatter conduction band induced by the longer acetylene links. Meanwhile, longer acetylene links result in a larger red shift of the imaginary part of the dielectric function, loss function, and extinction coefficient. In this work, we propose an effective method to tune and manipulate both the electronic and optical properties of graphynes for the applications in optoelectronic devices and photochemical catalysis.
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http://dx.doi.org/10.1021/acsomega.1c00840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153916PMC
April 2021

Screening, construction, and serological identification of the diagnostic antigen molecule EG-06283 for the diagnosis of Echinococcus granulosus.

Parasitol Res 2021 May 27. Epub 2021 May 27.

Scientific Technology Center of Ningxia Medical University, Yinchuan, 750004, Ningxia, China.

Several strategies exist to prevent and control echinococcosis, a global parasitic disease. However, most treatments are ineffective and adverse effects are common. Therefore, we aimed to screen protoscolex antigen molecules of Echinococcus granulosus to identify a diagnostic biomarker for hydatid disease. Published E. granulosus transcriptome sequencing data were analyzed to screen for antigen molecules that are highly expressed in protoscoleces but not in oncospheres. The membrane protein EG-06283 (annotated as Frizzled-4) was selected from 16 antigens, and its gene fragment was subjected to codon optimization and synthesis. rEG-06283 expression was induced in the pET-24a/EG-06283/BL21 strain; subsequently, the protein was purified and subcutaneously injected into ICR mice at weeks 0, 2, 4, and 6. Blood sampling occurred periodically to quantify serum immunoglobulin G (IgG) levels via enzyme-linked immunosorbent assays (ELISA). Immunogenicity was determined by western blot assays using sera from normal mice and mice with secondary hydatid infections. The antigen's immune reactivity and diagnostic value were validated using sera of patients with hydatid disease. ELISA results confirmed that the antigen molecule induced specific IgG production in mice, resulting in significantly higher levels than those in the adjuvant and control groups (P < 0.05). The western blot results indicated that the protein was recognized by antibodies in the sera of mice with hydatid infection and the antisera of immunized mice. Quantification of protein levels in the sera of patients with hydatid disease significantly differed from levels in healthy participants (P < 0.05). These results indicate that rEG-06283 is a potential diagnostic antigen for E. granulosus infections.
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http://dx.doi.org/10.1007/s00436-021-07183-yDOI Listing
May 2021

The differential demographic pattern of coronavirus disease 2019 fatality outside Hubei and from six hospitals in Hubei, China: a descriptive analysis.

BMC Infect Dis 2021 May 26;21(1):481. Epub 2021 May 26.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.

Background: The coronavirus disease 2019 (COVID-19) epidemic has been largely controlled in China, to the point where case fatality rate (CFR) data can be comprehensively evaluated.

Methods: Data on confirmed patients, with a final outcome reported as of 29 March 2020, were obtained from official websites and other internet sources. The hospitalized CFR (HCFR) was estimated, epidemiological features described, and risk factors for a fatal outcome identified.

Results: The overall HCFR in China was estimated to be 4.6% (95% CI 4.5-4.8%, P < 0.001). It increased with age and was higher in males than females. Although the highest HCFR observed was in male patients ≥70 years old, the relative risks for death outcome by sex varied across age groups, and the greatest HCFR risk ratio for males vs. females was shown in the age group of 50-60 years, higher than age groups of 60-70 and ≥ 70 years. Differential age/sex HCFR patterns across geographical regions were found: the age effect on HCFR was greater in other provinces outside Hubei than in Wuhan. An effect of longer interval from symptom onset to admission was only observed outside Hubei, not in Wuhan. By performing multivariate analysis and survival analysis, the higher HCFR was associated with older age (both P < 0.001), and male sex (both P < 0.001). Only in regions outside Hubei, longer interval from symptom onset to admission, were associated with higher HCFR.

Conclusions: This up-to-date and comprehensive picture of COVID-19 HCFR and its drivers will help healthcare givers target limited medical resources to patients with high risk of fatality.
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http://dx.doi.org/10.1186/s12879-021-06187-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153527PMC
May 2021