Publications by authors named "Qiang Wang"

4,230 Publications

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Native architecture and acclimation of photosynthetic membranes in a fast-growing cyanobacterium.

Plant Physiol 2022 Aug 10. Epub 2022 Aug 10.

Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.

Efficient solar energy conversion is ensured by the organization, physical association, and physiological coordination of various protein complexes in photosynthetic membranes. Here, we visualize the native architecture and interactions of photosynthetic complexes within the thylakoid membranes from a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) using high-resolution atomic force microscopy (AFM). In the Syn2973 thylakoid membranes, both photosystem I (PSI)-enriched domains and crystalline photosystem II (PSII) dimer arrays were observed, providing favorable membrane environments for photosynthetic electron transport. The high light (HL)-adapted thylakoid membranes accommodated a large amount of PSI complexes, without the incorporation of iron-stress-induced protein A (IsiA) assemblies and formation of IsiA-PSI supercomplexes. In the iron deficiency (Fe-)-treated thylakoid membranes, in contrast, IsiA proteins densely associated with PSI, forming the IsiA-PSI supercomplexes with varying assembly structures. Moreover, type-I NADH dehydrogenase-like complexes (NDH-1) were upregulated under the HL and Fe- conditions and established close association with PSI complexes to facilitate cyclic electron transport. Our study provides insight into the structural heterogeneity and plasticity of the photosynthetic apparatus in the context of their native membranes in Syn2973 under environmental stress. Advanced understanding of the photosynthetic membrane organization and adaptation will provide a framework for uncovering the molecular mechanisms of efficient light harvesting and energy conversion.
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http://dx.doi.org/10.1093/plphys/kiac372DOI Listing
August 2022

The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA pathways.

BMC Gastroenterol 2022 Aug 8;22(1):378. Epub 2022 Aug 8.

Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College, No. 287 Changhuai Road, Bengbu, 233030, Anhui, China.

Background: The stromal antigen 3 (STAG3) gene encodes an adhesion complex subunit that can regulate sister chromatid cohesion during cell division. Chromosome instability caused by STAG3 gene mutation may potentially promote tumor progression, but the effect of STAG3 on hepatocellular carcinoma (HCC) and the related molecular mechanism are not reported in the literature. The mechanism of the occurrence and development of HCC is not adequately understood. Therefore, the biological role of STAG3 in HCC remains to be studied, and whether STAG3 might be a sensitive therapeutic target in HCC remains to be determined.

Methods: The expression and clinical significance of STAG3 in HCC tissues and cell lines were determined by RT-qPCR and immunohistochemistry analyses. The biological functions of STAG3 in HCC were determined through in vitro and in vivo cell function tests. The molecular mechanism of STAG3 in HCC cells was then investigated by western blot assay.

Results: The mRNA expression of STAG3 was lower in most HCC cells than in normal cells. Subsequently, an immunohistochemical analysis of STAG3 was performed with 126 samples, and lower STAG3 expression was associated with worse overall survival in HCC patients. Moreover, cytofunctional tests revealed that the lentivirus-mediated overexpression of STAG3 in HCC cells inhibited cell proliferation, migration, and invasion; promoted apoptosis; induced G1/S phase arrest in vitro; and inhibited tumor growth in vivo. Furthermore, studies of the molecular mechanism suggested that the overexpression of STAG3 increased Smad3 expression and decreased CDK4, CDK6, cyclin D1, CXCR4 and RhoA expression.

Conclusion: STAG3 exhibits anticancer effects against HCC, and these effects involve the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA pathways. STAG3 is a tumor-suppressor gene that may serve as a potential target for molecular therapy, which provides a new idea for the treatment of HCC.
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http://dx.doi.org/10.1186/s12876-022-02400-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361574PMC
August 2022

Effects and mechanisms of GSG2 in esophageal cancer progression.

J Cancer Res Clin Oncol 2022 Aug 8. Epub 2022 Aug 8.

Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Jiangsu Province, No.188 Shizi street, Suzhou, 215006, China.

Background: Esophageal cancer was recognized as one of the malignant tumors with poor prognosis. Germ cell associated 2 (GSG2) has been reported to be of great significance in cell growth and tumor formation. This study aimed to investigate the biological function and molecular mechanism of GSG2 in esophageal cancer.

Methods: First, relationship between GSG2 expression and tumor characteristics in esophageal cancer patients was analyzed through immunohistochemical (IHC) staining. MTT assay, flow cytometry, cloning formation assay, wound-healing assay and Transwell assay were used to determine proliferation, apoptosis and migration of esophageal cancer cell with GSG2 knockdown in vitro. Expression of apoptosis related proteins and downstream pathway proteins after GSG2 knockdown were detected through Human Apoptosis Antibody Array and western blot analysis. The GSG2 knockdown function in vivo was explored through a xenograft tumor model.

Results: GSG2 was highly expressed in tumor tissues, which has clinical significance in predicting the malignant degree of patients with esophageal cancer. In addition, GSG2 knockdown significantly inhibited a variety of malignant biological behaviors of esophageal cancer cells, such as inhibiting proliferation, reducing colony formation, promoting apoptosis, hindering migration. The decrease of GSG2 expression in esophageal cancer cells can inhibit the xenograft tumor growth.

Conclusions: In conclusion, GSG2 was involved in esophageal cancer progression and development, which may provide an effective molecular target for the treatment of esophageal cancer in the future.
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http://dx.doi.org/10.1007/s00432-022-04260-2DOI Listing
August 2022

Human Umbilical Cord Wharton Jelly Cells Treatment Prevents Osteoporosis Induced by D-Galactose.

Int J Clin Pract 2022 20;2022:4593443. Epub 2022 Jul 20.

Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Rd., Shanghai 200120, China.

Methods: Sixteen male mice were randomly divided into 4 groups: control (ordinary feeding), D-gal (D-galactose) group, D-gal + MSC (human umbilical cord Wharton jelly cells), and D-gal + MSC-TNF groups. Except for the control group (fed with same amount of saline solution), other mice received gastric feeding of 250 mg/kg D-galactose every day for 8 weeks. TNF (10 ng/mL for 24 h) cocultured or noncocultured HUCWJCs (5 × 10) were suspended in 0.1 ml of sterile PBS and injected into tail veins every other week in D-gal + MSC-TNF and D-gal + MSC groups, respectively, and only 0.1 ml of sterile PBS for control and D-gal groups. The bone mass was detected by qPCR, ELISA, microcomputed tomography (CT), and hematoxylin-eosin staining. Proliferation, apoptosis, and differentiation of periosteal-derived osteoblasts (POB) were assessed. Transwell assay and scratch healing were performed to detect POB migration and invasion ability. The effect of HUCWJCs on POB signaling pathway expression was evaluated by immunoblotting.

Results: The malondialdehyde (MDA) in serum was higher and superoxide dismutase (SOD) was lower in the D-gal group compared to the other groups ( < 0.05). Mice in D-gal group showed significantly decreased bone mass when compared to the control group, while HUCWJCs treatment partially rescued the phenotype, as demonstrated by CT and histology ( < 0.05). Mechanically, HUCWJCs treatment partially promoted proliferation and migration and decreased apoptosis of POB induced by oxidative stress via activating the mitogen-activated protein kinase (MAPK) signaling pathway.

Conclusion: HUCWJCs can prevent the progression of osteoporosis by inhibiting oxidative stress, which may act by regulating osteoblasts fate through the MAPK signaling pathway.
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http://dx.doi.org/10.1155/2022/4593443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328953PMC
August 2022

Resting-state electroencephalography of neural oscillation and functional connectivity patterns in late-life depression.

J Affect Disord 2022 Aug 2. Epub 2022 Aug 2.

Department of Geriatric Psychiatry, the Affiliated Brain Hospital of Guangzhou Medical University, China. Electronic address:

Background: The clinical manifestations of late-life depression (LLD) are highly heterogeneous. Currently, abnormal characteristics of resting-state electroencephalography (EEG) power and functional connectivity are considered trait markers of depressive symptoms in major depression. However, the relationship between EEG spectral features and functional connectivity in LLD remains unknown.

Methods: Forty-four patients with LLD and 41 participants without depression underwent an eyes-closed resting-state EEG. EEG power spectra, alpha asymmetry, and functional connectivity were calculated and analyzed.

Results: Although alpha frontal asymmetry and cortical functional connectivity between the two groups showed no significant differences, the LLD group exhibited abnormal neural oscillation patterns of higher beta frequency activity in the parietal, central, and occipital lobes while alpha activity was increased in the parietal central electrodes.

Limitations: The number of EEG electrodes used in this study was low, and the sample size was limited.

Conclusions: Increased alpha and beta frequency band powers were observed in patients with LLD. These abnormal patterns may be associated with a disturbed balance of cortical excitation, inhibition, and hyperactivity. In the future, a neurofeedback protocol based on the findings of neural oscillation patterns in certain types of LLD should be explored.
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http://dx.doi.org/10.1016/j.jad.2022.07.055DOI Listing
August 2022

A simple and practical intraoperative ventilation technique for uniportal video-assisted thoracoscopic tracheal reconstruction: a case report.

Ann Transl Med 2022 May;10(9):529

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Cross-field endotracheal intubation is typically performed during tracheal anastomosis to maintain single-lung ventilation. To minimize obstruction of the surgical field by the cross-field tube, special equipment such as high-frequency jet ventilation (HFJV) and extracorporeal membrane oxygenation (ECMO) or advanced techniques such as non-intubated ventilation have been proposed. Here, we describe a simple and practical airway management strategy that requires only conventional ventilators and techniques. Our operation is completed under uniportal video-assisted thoracoscopic surgery (VATS).

Case Description: We report a case of tracheal adenoid cystic carcinoma (ACC) presenting with cough with bloody sputum in a 53-year-old man. Computed tomography (CT) and flexible bronchoscopy revealed an irregular polypoid neoplasm attached to the right wall of the distal trachea, which almost completely blocked the tracheal lumen. To relieve the symptoms, transbronchoscopic resection of the tumor, followed by curative resection via uniportal VATS under general anesthesia was performed. To maintain single-lung ventilation during tracheal reconstruction, we took advantage of a thin suction tube [internal diameter (ID) 3 mm; external diameter (ED) 4 mm], which was connected to a conventional ventilator. Specifically, by introducing the suction tube into the distal left main bronchus through the endotracheal tube and blowing 100% oxygen, we achieved satisfactory oxygenation throughout the anastomotic process; and the blood CO partial pressure was also acceptable. The view of the anastomotic site was far less obstructed owing to the small diameter of the suction tube, and the anastomotic process was smooth and accurate. Postoperative recovery was good, and no stenosis of the reconstructed trachea was observed at the 3-month follow-up.

Conclusions: Our technique proves to be safe and feasible for selected patients with tracheal tumors, and can be a practical choice for medical centers that are not equipped with HFJV or ECMO.
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http://dx.doi.org/10.21037/atm-21-6215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347034PMC
May 2022

An Overview of Neurological and Psychiatric Complications During Post-COVID Period: A Narrative Review.

J Inflamm Res 2022 26;15:4199-4215. Epub 2022 Jul 26.

Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, People's Republic of China.

The coronavirus disease 2019 (COVID-19), induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a multi-organ and multi-system disease with high morbidity and mortality in severe cases due to respiratory failure and severe cardiovascular events. However, the various manifestations of neurological and psychiatric (N/P) systems of COVID-19 should not be neglected. Some clinical studies have reported a high risk of N/P disorders in COVID-19 and post-COVID-19 patients and that their outcomes were positively associated with the disease severity. These clinical manifestations could attribute to direct SARS-CoV-2 invasion into the central nervous system (CNS), which is often complicated by systemic hypoxia, the dysfunctional activity of the renin-angiotensin system and other relevant pathological changes. These changes may remain long term and may even lead to persistent post-COVID consequences on the CNS, such as memory, attention and focus issues, persistent headaches, lingering loss of smell and taste, enduring muscle aches and chronic fatigue. Mild confusion and coma are serious adverse outcomes of neuropathological manifestations in COVID-19 patients, which could be diversiform and vary at different stages of the clinical course. Although lab investigations and neuro-imaging findings may help quantify the disease's risk, progress and prognosis, large-scale and persistent multicenter clinical cohort studies are needed to evaluate the impact of COVID-19 on the N/P systems. However, we used "Boolean Operators" to search for relevant research articles, reviews and clinical trials from PubMed and the ClinicalTrials dataset for "COVID-19 sequelae of N/P systems during post-COVID periods" with the time frame from December 2019 to April 2022, only found 42 in 254,716 COVID-19-related articles and 2 of 7931 clinical trials involved N/P sequelae during post-COVID periods. Due to the increasing number of infected cases and the incessant mutation characteristics of this virus, diagnostic and therapeutic guidelines for N/P manifestations should be further refined.
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http://dx.doi.org/10.2147/JIR.S375494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342586PMC
July 2022

Elevated homocysteine levels, white matter abnormalities and cognitive impairment in patients with late-life depression.

Front Aging Neurosci 2022 18;14:931560. Epub 2022 Jul 18.

Center for Geriatric Neuroscience, The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Cognitive impairment in late-life depression (LLD) is considered to be caused by neurodegenerative changes. Elevated homocysteine (Hcy) levels may be linked to cognitive abnormalities associated with LLD. The important role of white matter (WM) damage in cognitive impairment and pathogenesis in patients with LLD has been widely reported. However, no research has explored the interrelationships of these features in patients with LLD.

Objective: The goal of the study was to examine the interrelationship between Hcy levels, cognition, and variations in WM microstructure detected by diffusion tensor imaging (DTI) in patients with LLD.

Methods: We recruited 89 healthy controls (HCs) and 113 patients with LLD; then, we measured the plasma Hcy levels of participants in both groups. All individuals performed a battery of neuropsychological tests to measure cognitive ability. Seventy-four patients with LLD and 68 HCs experienced a DTI magnetic resonance imaging (MRI) scan.

Results: Patients with LLD showed significantly lower fractional anisotropy (FA) values in the bilateral inferior longitudinal fasciculus than those of healthy participants. Only in LLD patients was Hcy concentration inversely associated to FA values in the forceps minor. Finally, multiple regression analyses showed that an interaction between Hcy levels and FA values in the right cingulum of the cingulate cortex and right inferior longitudinal fasciculus were independent contributors to the executive function of patients with LLD.

Conclusion: Our results highlight the complex interplay between elevated homocysteine levels and WM abnormalities in the pathophysiology of LLD-related cognitive impairment, consistent with the neurodegeneration hypothesis.
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http://dx.doi.org/10.3389/fnagi.2022.931560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340773PMC
July 2022

One-step high-yield preparation of nitrogen- and sulfur-codoped carbon dots with applications in chromium(vi) and ascorbic acid detection.

RSC Adv 2022 Jun 13;12(30):19686-19694. Epub 2022 Jul 13.

College of Food and Biological Engineering, Jimei University Xiamen 361021 P. R. China

In this research, a nitrogen- (N) and sulfur- (S) codoped carbon dot (CDs-IPM)-based sensor was synthesized using a single-step hydrothermal method. Specifically, microcrystalline cellulose (MCC) was the main raw material, which was extracted from banana pseudo-stem-based waste, while autonomous sulfonic acid-functionalized ionic liquid (SOH-IL) and polyethylene glycol 400 (PEG 400) acted as the N, S dopant, and surface modifier, respectively. Comprehensive spectroscopic characterization of the synthesized CDs-IPM revealed the introduction of S, N atoms in the matrix with existence of surface oxygenic functional groups. The CDs-IPM possessed enhanced photoluminescence (PL) intensity, synthetic yield, and PL quantum yield (PLQY). Additionally, electron transfer between the CDs-IPM, hexavalent chromium (Cr(vi)), and subsequent ascorbic acid (AA) succeeded in turning the fluorescence on and off. The detection limit was 17 nM for Cr(vi), while it was 103 nM for AA. Our study data can simplify the process of synthesis of CDs utilizing biodegradable starting materials. The probe reported in this study may serve as a valuable addition to the field of environment monitoring by virtue of its enhanced detection sensitivity, high selectivity, and stability.
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http://dx.doi.org/10.1039/d2ra01758jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277421PMC
June 2022

The pattern glare and visual memory are disrupted in patients with major depressive disorder.

BMC Psychiatry 2022 08 2;22(1):518. Epub 2022 Aug 2.

Psychiatric Laboratory and Mental Health Center, West China Hospital of Sichuan University, Chengdu, China.

Background: Visual memory impairment is one of the most commonly complained symptoms in patients with major depressive disorder (MDD). Pattern glare is also a distorted visual phenomenon that puzzles patients with MDD. Nevertheless, how these two phenomena interact in MDD remains unknown. This study investigated the association between pattern glare and visual memory in MDD patients.

Methods: Sixty-two patients with MDD and forty-nine age-, sex- and education level-matched healthy controls (HCs) were included in this study. The Pattern Recognition Memory (PRM) test and the Brief Visual Memory Test-Revised (BVMT-R) were applied to measure visual memory. The pattern glare test including three patterns with different spatial frequencies (SFs) was used to explore pattern glare levels.

Results: Patients with MDD scored lower on the PRM-PCi, BVMT-R1, BVMT-R2, BVMT-R3, and BVMT-Rt and higher on the PRM-MCLd than HCs (all p < 0.05). Pattern glare scores for MDD patients were higher with mid-SF (p < 0.001), high-SF (p = 0.006) and mid-high SF differences (p = 0.01) than for HCs. A positive correlation between mid-SF and PRM-MCLd scores in all participants was observed (p = 0.01, r = 0.246). A negative correlation between mid-high difference scores and BVMT-R2 scores (p = 0.032, r = -0.317) was observed in HCs, but no significant correlation was observed in MDD patients.

Conclusions: The present study showed that visual memory and pattern glare are disrupted in MDD. Visual memory may be associated with pattern glare and needs to be studied in future work.
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http://dx.doi.org/10.1186/s12888-022-04167-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344705PMC
August 2022

PDGFR kinase inhibitor protects against septic death via regulation of BTLA.

Sci China Life Sci 2022 Sep 29. Epub 2022 Sep 29.

Department of Trauma Medical Center, Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Army Medical University, Chongqing, 400042, China.

Sepsis, defined as life-threatening organ failure caused by a dysregulated host response to severe infection, is a major cause of death among intensive care unit patients. Therapies targeting on immunomodulatory is a new research field in sepsis treatment. B- and T-lymphocyte attenuator (BTLA) is an inhibitory costimulatory factor molecule of B and T lymphocytes. Studies have shown that elevated expression of BTLA in lymphocytes can reduce mortality in sepsis, but its regulatory compounds and the underlying mechanism remains to be elucidated. Here, we show that treatment with CP-673451 significantly decreases mortality of septic mouse. CP-673451 is a PDGFR kinase inhibitor which can promote the expression of BTLA, inhibit the release of chemokines such as CXCL13, and reduce first the chemotaxis of B cells to the peripheral blood and vital organs. CP-673451 also inhibits both the release of cytokines and chemokines such as IL-1β, IL-6, IL-10, TNF-α, CCL1, CCL2 and CCL7 and reduces both the chemotactic ability of T cells. This suggests that CP-673451 may prevent septic death by inhibiting lymphocyte chemotaxis and alleviating "cytokine storm". In conclusion, our study provides a new therapeutic target and an effective compound for sepsis treatment.
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http://dx.doi.org/10.1007/s11427-021-2136-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345782PMC
September 2022

Electronic-Level Insight into Interfacial Effects and Their Induced Anisotropic Ion Diffusion and Ion Selectivity in Nanochannels.

ACS Appl Mater Interfaces 2022 Aug 2. Epub 2022 Aug 2.

MOE Key Laboratory of Thermal-Fluid Science and Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.

Osmotic energy conversion features directional ion migration in selective nanochannels, dominated by interfacial effects, temperature, and concentration. Current efforts emphasize membrane modification for superior reliability and durability, whereas the origin and implication of interfacial effects are unclear. This work performs ab initio molecular dynamics simulations for hydrated ion-graphene oxide interfaces by regulating the temperature and concentration. The interfacial effects associated with their induced anisotropic ion diffusion and ion selectivity are revealed. The scientific essence of the interfacial effects is an electron transfer triggered by hydrated ion-functional group interactions. The interfacial effects are clarified to include dynamic solvation structures, interfacial H-bonds, and chemical reactions. Ions possess incomplete hydration shells, and their arrangements vary from ordered to disordered to overlapped. Interfacial H-bonds restrict hydrated ions by constraining water molecules, whereas continuous reactions provide lateral pathways to generate anisotropy. Cation selectivity is further clarified by negative surface charges from hydroxyl deprotonation. Besides, temperature rise induces disordered hydrated ions as well as frequent and violent reactions, enhancing ion diffusion, selectivity, and anisotropy; excessive concentrations produce overlapped hydrated ions, more H-bonds, and inferior reactions, weakening ion diffusion, selectivity, and anisotropy. Finally, the bottom-up concept for osmotic energy conversion is summarized, and elevated temperature combined with low concentration is found to boost ion diffusion and ion selectivity synergistically. This work provides an in-depth understanding of interfacial phenomena and ion behaviors in nanochannels.
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http://dx.doi.org/10.1021/acsami.2c06687DOI Listing
August 2022

Operating interfaces to synthesize [email protected] nanoparticles by modifying the heating process.

Nanoscale 2022 Aug 2. Epub 2022 Aug 2.

Key Laboratory of Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China.

A facile strategy to operate interfaces when synthesizing [email protected] nanoparticles (NPs) has been proposed. Two interfaces are indispensable to obtain the high ordering L1-FePt structure. One is the mismatched interfaces between the initial γ-PtBi nuclei and the disordered fcc-FePt phase. The other is the grown coherent interfaces between the L1-FePt and Bi-rich phases. Increasing the heating rates when the temperature rises from 120 °C to 310 °C benefits the formation of mismatched interfaces and improves the uniformity of phases and composition in NPs. Reducing the heating rate at higher temperature ensures sufficient time for Bi to diffuse across the coherent interface, which facilitates the disorder-order transition of L1-FePt NPs. This study provides a new perspective on operating interfaces during the wet-chemical synthesis process.
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http://dx.doi.org/10.1039/d2nr01493aDOI Listing
August 2022

LINC02418 upregulates EPHA2 by competitively sponging miR-372-3p to promote 5-Fu/DDP chemoresistance in colorectal cancer.

Carcinogenesis 2022 Aug 1. Epub 2022 Aug 1.

Institute of Infection, Immunology and Tumor Microenvironment, School of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China.

Chemoresistance is a huge clinical challenge in the treatment of advanced colorectal cancer (CRC). Non-coding RNAs (ncRNAs) and messenger RNA (mRNA) are involved in CRC chemoresistance. However, the profiles of long ncRNAs (lncRNAs), microRNAs (miRNAs), mRNAs, and competing endogenous RNA (ceRNA) networks in CRC chemoresistance are still largely unknown. Here, we compared the gene expression profiles in chemo-sensitive (HCT8) and chemo-resistant (HCT8/5-fluorouracil (5-Fu) and HCT8/cisplatin (DDP)) cell lines by whole-transcriptome sequencing. The common differentially expressed (DE) RNAs in two drug-resistant cells were selected to construct lncRNA-miRNA-mRNA networks. The ceRNA network closely related to chemoresistance was further established based on the widely accepted drug resistance-associated genes enriched in three signaling pathways involved in chemoresistance. In total 52 lncRNA-miRNA-mRNA pathways were screened out, among which EPHA2 and LINC02418 were identified as hub genes; thus, LINC02418/miR-372-3p/EPHA2 were further selected and proved to affect the 5-Fu and DDP resistance of CRC. Mechanistically, LINC02418 upregulated EPHA2 by functioning as a "sponge" of miR-372-3p to modulate the chemoresistance of CRC. Collectively, our study uncovered the underlying mechanism of LINC02418/miR-372-3p/EPHA2 in 5-Fu and DDP resistance of CRC, which may provide potential therapeutic targets for improving the chemosensitivity of CRC.
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http://dx.doi.org/10.1093/carcin/bgac065DOI Listing
August 2022

Patterns of Convergence and Divergence Between Bipolar Disorder Type I and Type II: Evidence From Integrative Genomic Analyses.

Front Cell Dev Biol 2022 15;10:956265. Epub 2022 Jul 15.

Mental Health Center and Psychiatric Laboratory, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.

Genome-wide association studies (GWAS) analyses have revealed genetic evidence of bipolar disorder (BD), but little is known about the genetic structure of BD subtypes. We aimed to investigate the genetic overlap and distinction of bipolar type I (BD I) & type II (BD II) by conducting integrative post-GWAS analyses. We utilized single nucleotide polymorphism (SNP)-level approaches to uncover correlated and distinct genetic loci. Transcriptome-wide association analyses (TWAS) were then approached to pinpoint functional genes expressed in specific brain tissues and blood. Next, we performed cross-phenotype analysis, including exploring the potential causal associations between two BD subtypes and lithium responses and comparing the difference in genetic structures among four different psychiatric traits. SNP-level evidence revealed three genomic loci, , and , shared by BD I and II, and one locus () and significant gene sets involved in calcium channel activity, neural and synapsed signals that distinguished two subtypes. TWAS data implicated different genes affecting BD I and II through expression in specific brain regions (nucleus accumbens for BD I). Cross-phenotype analyses indicated that BD I and II share continuous genetic structures with schizophrenia and major depressive disorder, which help fill the gaps left by the dichotomy of mental disorders. These combined evidences illustrate genetic convergence and divergence between BD I and II and provide an underlying biological and trans-diagnostic insight into major psychiatric disorders.
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http://dx.doi.org/10.3389/fcell.2022.956265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334650PMC
July 2022

Personality traits influence the effectiveness of hypomania checklist-32 in screening for bipolar disorder.

Front Psychiatry 2022 15;13:919305. Epub 2022 Jul 15.

Department of Medical Psychology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: It is clinically challenging to distinguish bipolar disorder (BD) from major depressive disorder (MDD) in the early stages. While the hypomania checklist-32 (HCL-32) is a proper auxiliary tool that is useful to differentiate between BD and MDD, there is currently no standard cut-off value. The variations in HCL-32 cut-off values could potentially be influenced by personality traits. Therefore, the aim of this study is to explore the effect of personality traits on the screening performance of HCL-32.

Methods: In this retrospective cross-sectional study, 168 patients with BD or MDD were evaluated with the Eysenck Personality Questionnaire (EPQ) and HCL-32. The associations between demographic data, diagnosis and clinical rating scales were analyzed.

Results: Diagnosis was not associated with extraversion but was related to neuroticism. HCL-32 scores in typical extraverted patients were higher in contrast to atypical extraverted patients. The best cut-off value for BD recognition of typical and atypical extraversion groups were 15 and 12.5, respectively. In patients with MDD, HCL-32 score of typical neuroticism was higher than the atypical type, but there was no difference in patients with BD. In typical neuroticism, there was no difference in HCL-32 scores between patients with MDD and BD. But among atypical neurotic patients, HCL-32 scores of BD were higher compared to MDD, with a cut-off value of 14.5.

Limitations: This study had a small sample size.

Conclusion: HCL-32 scores were affected by personality traits, with higher scores for typical extraversion and neuroticism. Clinicians should also consider the patients' personality traits when referring to HCL-32 scores, so as to increase the recognition rate of BD and eliminate false positives.
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http://dx.doi.org/10.3389/fpsyt.2022.919305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337268PMC
July 2022

LncRNA NBR2 regulates cancer cell stemness and predicts survival in non-small cell cancer patients by downregulating TGF-β1.

Curr Pharm Biotechnol 2022 Jul 28. Epub 2022 Jul 28.

Department of Cardio-thoracic surgery, Wujin Hospital Affiliated with Jiangsu University (The Wujin Clinical College of Xuzhou Medical University), Changzhou City, Jiangsu Provence,213017, China.

Background: LncRNA NBR2 is a key regulator in cancer metabolism. However, its role in lung cancer is unknown.

Objective: This study aimed to explore the function of NBR2 in non-small cell lung cancer (NSCLC), which is the most common type of lung cancer.

Materials And Methods: Paired NSCLC and non-cancer tissues were collected form 68 patients with NSCLC. Expression of NBR2 and transforming growth factor-β1 (TGF-β1) in these samples was analyzed by RT-qPCR. The prognostic value of NBR2 for NSCLC was explored by performing a 5-year follow-up study. The interaction between NBR2 and TGF-β1 in two NSCLC cell lines was detected by overexpression assay, followed by RT-qPCR and Western blot analysis. Flow cytometry was performed to evaluate the role of NBR2 and TGF-β1 in regulating NSCLC cell stemness.

Results: NBR2 was significantly downregulated in NSCLC tissues than that in non-cancer tissues of NSCLC patients, and low expression levels of NBR2 predicted poor survival. TGF-β1 was significantly upregulated in NSCLC tissues than that in non-cancer tissues, and was inversely correlated with NBR2. Overexpression of NBR2 downregulated TGF-β1, while overexpression of TGF-β1 did not affect the expression of NBR2. Overexpression of NBR2 inhibited, while overexpression of TGF-β1 promoted NSCLC cell stemness. Overexpression of TGF-β1 attenuated the effects of overexpression of NBR2. Mechanically, NBR2 interacted with Notch1 protein to inhibit its expression, thereby inhibiting the expression of TGF-β1 and further affecting the proportion of CD133+ cells.

Conclusion: LncRNA NBR2 regulates cancer cell stemness and predicts survival in NSCLC possibly by downregulating TGF-β1 through Notch1.
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http://dx.doi.org/10.2174/1389201023666220728143410DOI Listing
July 2022

Inhibiting SRC activity attenuates kainic-acid induced mouse epilepsy via reducing NR2B phosphorylation and full-length NR2B expression.

Epilepsy Res 2022 Jul 8;185:106975. Epub 2022 Jul 8.

Department of Neurology, Zhongshan Hospital, Fudan University, Fenglin Road, Shanghai 200032, China; Department of The State Key Laboratory of Medical Neurobiology, The Institutes of Brain Science and the Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China. Electronic address:

Objective: To explore the effect of SRC activation on spontaneously recurrent seizures and to investigate the underlying mechanisms of NR2B phosphorylation.

Methods: C57BL/6 mice were injected intrahippocampally with kainic acid (KA, 0.4 μg/25 g) to induce status epilepticus (SE). Saracatinib(STB) was used as an SRC inhibitor. Spontaneously recurrent seizures were monitored from day 7 to day 14 after the KA injection. Nissl's stain and NeuN were used to detect neuron loss and Timm stain was used to evaluate mossy fibre sprouting 14 days after KA injection. We also investigated the effect of SRC on full-length expression of NR2B. MDL28170 was used to inhibit calpain activity. Western blotting and qPCR were performed to verify phosphorylation levels and expression of SRC and NR2B 24 h after KA injection.

Results: The duration of status epileptics in the SRC inhibitor group decreased significantly compared to the KA group 24 h after the injection of KA (P < 0.05). The application of the SRC inhibitor significantly reduced the degree of contralateral mossy fibre sprouting (P < 0.05) and improved the degree of neuron loss (P < 0.01) compared to the epilepsy group. Full-length NR2B levels in the ipsilateral hippocampus decreased in the epilepsy group (P < 0.01) compared to the sham group, and it further decreased in the STB inhibitor group (P < 0.01). The effect of the STB inhibitor was counteracted by simultaneous inhibition of SRC activity and calpain activation, while the level of full-length NR2B increased compared to the KA+STB group(P < 0.01). Reduction of NR2B cleavage by MDL28170 significantly increased the duration of epileptic status compared to the KA group (P < 0.05).

Significance: Our data indicated that the early application of SRC inhibitors exerted protective effects on seizure severity, loss of neurons, and sprouting of mossy fibres in KA-induced mouse epilepsy. Seizure severity attenuation due to SRC inhibition was associated with the decrease of NR2B in both the phosphorylation and full-length forms.
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http://dx.doi.org/10.1016/j.eplepsyres.2022.106975DOI Listing
July 2022

The promoting/inhibiting effect of water vapor on the selective catalytic reduction of NO.

J Hazard Mater 2022 Jul 22;439:129665. Epub 2022 Jul 22.

Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address:

In the field of nitrogen oxides (NO) abatement, developing selective catalytic reduction (SCR) catalysts that can operate stably in the practical conditions remains a big challenge because of the complexity and uncertainty of actual flue gas emissions. As water vapor is unavoidable in the actual flue gas, it is indispensable to explore its effect on the performance of SCR catalysts. Many studies have proved that the effects of HO on de-NO activity of SCR catalysts were indeed observed during SCR reactions operated under wet conditions. Whether the effect is promotive or inhibitory depends on the reaction conditions, catalyst types and reducing agents used in SCR reaction. This review focuses on the effect of HO on SCR catalysts and SCR reaction, including promoting effect, inhibiting effect, as well as the effecting mechanism. Besides, various strategies for developing a water-resistant SCR catalyst are also included. We hope that this work can give a more comprehensive insight into the effects of HO on SCR catalysts and help with the rational design of water-resistant SCR catalysts for further practical application in NO abatement field.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129665DOI Listing
July 2022

Characterizing Complex Populations of Endogenous Adeno-associated Viruses by Single-Genome Amplification.

Hum Gene Ther 2022 Jul 29. Epub 2022 Jul 29.

University of Pennsylvania Perelman School of Medicine, Gene Therapy Program, Suite 1200 TRL, 125 S. 31st Street, Philadelphia, Pennsylvania, United States, 19104;

The isolation of adeno-associated virus (AAV) genomes from biomaterials at the molecular level has traditionally relied on polymerase chain reaction-based and cloning-based techniques. However, when applied to samples containing multiple species, traditional techniques for isolating viral genomes can amplify artificial recombinants and introduce polymerase misincorporation errors. Here, we describe AAV single-genome amplification (AAV-SGA): a powerful technique to isolate, amplify, and sequence single AAV genomes from mammalian genomic DNA, which can then be used to construct vectors for gene therapy. We used AAV-SGA to precisely isolate 15 novel AAV genomes belonging AAV clades A, D, and E and the Fringe outgroup. This technique also enables investigations of AAV population dynamics and recombination events to provide insights into virus-host interactions and virus biology. Using AAV-SGA, we identified regional heterogeneity within AAV populations from different lobes of the liver of a rhesus macaque and found evidence of frequent genomic recombination between AAV populations. This study highlights the strengths of AAV-SGA and demonstrates its capability to provide valuable insights into the biology and diversity of AAVs.
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http://dx.doi.org/10.1089/hum.2022.099DOI Listing
July 2022

Influence of zeolite confinement effects on cation-π interactions in methanol-to-hydrocarbon conversion.

Chem Commun (Camb) 2022 Jul 28. Epub 2022 Jul 28.

National Center for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.

By using 2D C-C correlation MAS NMR spectroscopy and DFT calculations, the nature of cation-π interactions between cyclopentenyl cations and benzene was clarified over H-ZSM-5, H-β and H-SSZ-13 zeolites. The cation-π interactions are favored over H-β and H-SSZ-13 with large channels or cages. The zeolite structure is identified to affect the arrangements of cyclopentenyl cations and benzene in the confined environment, leading to different extents of overlapping of positive-negative charge centers and cation-π interaction strength. The stronger cation-π interactions facilitate the bimolecular reactions between cyclopentenyl cations and benzene and the formation of coke species.
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http://dx.doi.org/10.1039/d2cc02216hDOI Listing
July 2022

Circulatory Metabolomics Reveals the Association of the Metabolites With Clinical Features in the Patients With Intrahepatic Cholestasis of Pregnancy.

Front Physiol 2022 11;13:848508. Epub 2022 Jul 11.

Department of Gynecology and Obstetrics, International Collaborative Research Center for Medical Metabolomics, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University, Changsha, China.

Intrahepatic cholestasis of pregnancy (ICP) is associated with an increased risk of adverse pregnancy to the mother and fetus. As yet, the metabolic profiles and the association of the clinical features remain obscure. Fifty-seven healthy pregnant women and 52 patients with ICP were recruited in this study. Plasma samples were collected from pregnancies who received prenatal care between 30 and 36 weeks. Untargeted metabolomics to portray the metabolic profiles were performed by LC/MS. Multivariate combined with the univariate analysis was performed to screen out differential metabolites between the ICP and control groups. A de-biased sparse partial correlation (DSPC) network analysis of differential metabolites was conducted to explore the potential mutual regulation among metabolites on the basis of de-sparsified graphical lasso modeling. The pathway analysis was carried out using MetaboAnalyst. Linear regression and Pearson correlation analysis was applied to analyze correlations of bile acid levels, metabolites, newborn weights, and pregnancy outcomes in ICP patients. Conspicuous metabolic changes and choreographed metabolic profiles were disclosed: 125 annotated metabolites and 18 metabolic pathways were disturbed in ICP patients. DSPC networks indicated dense interactions among amino acids and their derivatives, bile acids, carbohydrates, and organic acids. The levels of total bile acid (TBA) were increased in ICP patients with meconium-stained amniotic fluid (MSAF) compared with those without MSAF. An abnormal tryptophan metabolism, elevated long chain saturated fatty acids and estrone sulfate levels, and a low-antioxidant capacity were relevant to increased bile acid levels. Newborn weights were significantly associated with the levels of bile acids and some metabolites of amino acids. Our study revealed the metabolomic profiles in circulation and the correlation of the metabolites with clinical features in ICP patients. Our data suggest that disturbances in metabolic pathways might be associated with adverse pregnancy outcomes.
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http://dx.doi.org/10.3389/fphys.2022.848508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309339PMC
July 2022

Synthesis and Biological Evaluation of Novel Allobetulon/Allobetulin-Nucleoside Conjugates as AntitumorAgents.

Molecules 2022 Jul 25;27(15). Epub 2022 Jul 25.

School of Medicine, Huanghe Science and Technology College, Zhengzhou 450063, China.

Allobetulin is structurally similar tobetulinic acid, inducing the apoptosis of cancer cells with low toxicity. However, both of them exhibited weak antiproliferation against several tumor cell lines. Therefore, the new series of allobetulon/allobetulin-nucleoside conjugates - were designed and synthesized for potency improvement. Compounds , , and showed promising antiproliferative activity toward six tested cell lines, compared to zidovudine, cisplatin, and oxaliplatin based on their antitumor activity results. Among them, compound exhibited much more potent antiproliferative activity against SMMC-7721, HepG2, MNK-45, SW620, and A549 human cancer cell lines than cisplatin and oxaliplatin. In the preliminary study for the mechanism of action, compound induced cell apoptosis and autophagy in SMMC cells, resulting in antiproliferation and G0/G1 cell cycle arrest by regulating protein expression levels of Bax, Bcl-2, and LC3. Consequently, the nucleoside-conjugated allobetulin () evidenced that nucleoside substitution was a viable strategy to improve allobetulin/allobetulon's antitumor activity based on our present study.
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http://dx.doi.org/10.3390/molecules27154738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329720PMC
July 2022

Arctic Ocean Amplification in a warming climate in CMIP6 models.

Sci Adv 2022 Jul 27;8(30):eabn9755. Epub 2022 Jul 27.

First Institute of Oceanography and Key Laboratory of Marine Science and Numerical Modeling, Ministry of Natural Resources, Qingdao, China.

Arctic near-surface air temperature warms much faster than the global average, a phenomenon known as Arctic Amplification. The change of the underlying Arctic Ocean could influence climate through its interaction with sea ice, atmosphere, and the global ocean, but it is less well understood. Here, we show that the upper 2000 m of the Arctic Ocean warms at 2.3 times the global mean rate within this depth range averaged over the 21st century in the Coupled Model Intercomparison Project Phase 6 Shared Socioeconomic Pathway 585 scenario. We call this phenomenon the "Arctic Ocean Amplification." The amplified Arctic Ocean warming can be attributed to a substantial increase in poleward ocean heat transport, which will continue outweighing sea surface heat loss in the future. Arctic Amplification of both the atmosphere and ocean indicates that the Arctic as a whole is one of Earth's regions most susceptible to climate change.
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http://dx.doi.org/10.1126/sciadv.abn9755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328679PMC
July 2022

Sharp Enhancement of Birefringence in Antimony Oxalates Achieved by the Cation-Anion Synergetic Interaction Strategy.

Inorg Chem 2022 Aug 27;61(31):12481-12488. Epub 2022 Jul 27.

College of Chemistry, Sichuan University, Chengdu 610065, P. R. China.

Birefringent materials with large birefringence play an important role in in laser science and technology owing to their ability to modulate polarized light. However, the lack of systematic and effective synthesis strategies severely hinders the development of novel superior birefringent materials. Herein, the cation-anion synergetic interaction strategy was proposed to successfully synthesize two excellent UV birefringent materials, RbSb(CO)F·HO and [C(NH)]Sb(CO)F·HO. Both compounds feature unprecedented [Sb(CO)F] anionic chains composed of planar π-conjugated [CO] units and a distorted SbOF complex with stereochemically active lone pairs, which induce a large optical anisotropy. Remarkably, further enhancement of birefringence in [C(NH)]Sb(CO)F·HO was achieved via cation-anion synergetic interactions between the [C(NH)] cationic groups and [Sb(CO)F] anionic chains. It exhibited a giant birefringence of [email protected] nm, twice larger than that of its analogue RbSb(CO)F·HO ([email protected] nm). A detailed structural analysis and theoretical calculations revealed that the cation-anion synergetic interaction strategy is an effective strategy for the efficient exploration of superior birefringent materials, which will guide the further exploration of new structure-driven functional materials.
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http://dx.doi.org/10.1021/acs.inorgchem.2c02262DOI Listing
August 2022

An Efficient LiDAR Point Cloud Map Coding Scheme Based on Segmentation and Frame-Inserting Network.

Sensors (Basel) 2022 Jul 7;22(14). Epub 2022 Jul 7.

College of Information and Control Engineering, Qingdao University of Technology, Qingdao 266525, China.

In this article, we present an efficient coding scheme for LiDAR point cloud maps. As a point cloud map consists of numerous single scans spliced together, by recording the time stamp and quaternion matrix of each scan during map building, we cast the point cloud map compression into the point cloud sequence compression problem. The coding architecture includes two techniques: intra-coding and inter-coding. For intra-frames, a segmentation-based intra-prediction technique is developed. For inter-frames, an interpolation-based inter-frame coding network is explored to remove temporal redundancy by generating virtual point clouds based on the decoded frames. We only need to code the difference between the original LiDAR data and the intra/inter-predicted point cloud data. The point cloud map can be reconstructed according to the decoded point cloud sequence and quaternion matrices. Experiments on the KITTI dataset show that the proposed coding scheme can largely eliminate the temporal and spatial redundancies. The point cloud map can be encoded to 1/24 of its original size with 2 mm-level precision. Our algorithm also obtains better coding performance compared with the octree and Google Draco algorithms.
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http://dx.doi.org/10.3390/s22145108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323153PMC
July 2022

Plasma Transthyretin Levels and Risk of Type 2 Diabetes Mellitus and Impaired Glucose Regulation in a Chinese Population.

Nutrients 2022 Jul 19;14(14). Epub 2022 Jul 19.

Department of Clinical Nutrition, Shandong Provincial Hospital, Shandong University, 324 Five Weft Seven Road, Jinan 250021, China.

Plasma transthyretin may be engaged in glucose regulation. We aimed to investigate the association between plasma transthyretin levels and the risk of newly diagnosed T2DM and impaired glucose regulation (IGR) in a Chinese population. We conducted a case-control study including 1244 newly diagnosed T2DM patients, 837 newly diagnosed IGR patients, and 1244 individuals with normal glucose tolerance (NGT) matched by sex and age. Multivariate logistic regression analysis was utilized to estimate the independent association of plasma transthyretin concentrations with the risk of T2DM and IGR. Plasma transthyretin concentrations were significantly higher in T2DM and IGR patients compared with control subjects ( < 0.005). After multiple adjustment and comparison with the lowest quartile of plasma transthyretin concentrations, the odds ratios (95% confidence intervals) of T2DM and IGR in the highest quartile were 2.22 (1.66, 2.98) and 2.29 (1.72, 3.05), respectively. Plasma transthyretin concentrations also showed a great performance in predicting the risk of T2DM (AUC: 0.76). Moreover, a potential nonlinear trend was observed. Our results demonstrated that higher plasma transthyretin concentrations, especially more than 290 mg/L, were associated with an increased risk of T2DM and IGR. Further studies are warranted to confirm our findings and elucidate the potential mechanisms.
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http://dx.doi.org/10.3390/nu14142953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321865PMC
July 2022

Quantitative Analysis of Drag Force for Task-Specific Micromachine at Low Reynolds Numbers.

Micromachines (Basel) 2022 Jul 18;13(7). Epub 2022 Jul 18.

Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Department of Mechanics and Engineering Structure, Wuhan University of Technology, Wuhan 430070, China.

Micromotors have spread widely in order to meet the needs of new applications, including cell operation, drug delivery, biosensing, precise surgery and environmental decontamination, due to their small size, low energy consumption and large propelling power, especially the newly designed multifunctional micromotors that combine many extra shape features in one device. Features such as rod-like receptors, dendritic biosensors and ball-like catalyzing enzymes are added to the outer surface of the tubular micromotor during fabrication to perform their special mission. However, the structural optimization of motion performance is still unclear. The main factor restricting the motion performance of the micromotors is the drag forces. The complex geometry of a micromotor makes its dynamic behavior more complicated in a fluid environment. This study aimed to design the optimum structure of tubular micromotors with minimum drag forces and obtain the magnitude of drag forces considering both the internal and external fluids of the micromotors. By using the computational fluid dynamics software Fluent 18.0 (ANSYS), the drag force and the drag coefficient of different conical micromotors were calculated. Moreover, the influence of the Reynolds numbers Re, the semi-cone angle δ and the ratios ξ and η on the drag coefficient was analyzed. The results show the drag force monotonically increased with Reynolds numbers Re and the ratio η. The extreme point of the drag curve is reached when the semi-cone angle δ is 8° and the ratio ξ is 3.846. This work provides theoretical support and guidance for optimizing the design and development of conical micromotors.
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http://dx.doi.org/10.3390/mi13071134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317653PMC
July 2022

Joint Detection of Community and Structural Hole Spanner of Networks in Hyperbolic Space.

Entropy (Basel) 2022 Jun 29;24(7). Epub 2022 Jun 29.

Wuhan Second Ship Design and Research Institute, Wuhan 430064, China.

Community detection and structural hole spanner (the node bridging different communities) identification, revealing the mesoscopic and microscopic structural properties of complex networks, have drawn much attention in recent years. As the determinant of mesoscopic structure, communities and structural hole spanners discover the clustering and hierarchy of networks, which has a key impact on transmission phenomena such as epidemic transmission, information diffusion, etc. However, most existing studies address the two tasks independently, which ignores the structural correlation between mesoscale and microscale and suffers from high computational costs. In this article, we propose an algorithm for simultaneously detecting communities and structural hole spanners via hyperbolic embedding (SDHE). Specifically, we first embed networks into a hyperbolic plane, in which, the angular distribution of the nodes reveals community structures of the embedded network. Then, we analyze the critical gap to detect communities and the angular region where structural hole spanners may exist. Finally, we identify structural hole spanners via two-step connectivity. Experimental results on synthetic networks and real networks demonstrate the effectiveness of our proposed algorithm compared with several state-of-the-art methods.
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http://dx.doi.org/10.3390/e24070894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319712PMC
June 2022

Mutation Association with Immune Checkpoint Inhibitor Outcome in Melanoma and Non-Small Cell Lung Cancer.

Cancers (Basel) 2022 Jul 19;14(14). Epub 2022 Jul 19.

Key Laboratory of Medicine and Health of Shandong Province, Department of Health Statistics, School of Public Health, Weifang Medical University, Weifang 261053, China.

Immune checkpoint inhibitors (ICIs) markedly promote the survival outcome of advanced melanoma and non-small cell lung cancer (NSCLC). Clinically, favorable ICI treatment efficacy is noticed only in a smaller proportion of patients. Heparan sulfate proteoglycan 2 (HSPG2) frequently mutates in both tumors. Herein, we aim to investigate the immunotherapeutic and immunological roles of mutations in melanoma and NSCLC. A total of 631 melanoma samples and 109 NSCLC samples with both somatic mutational profiles and clinical immunotherapy data were curated. In addition, by using The Cancer Genome Atlas data, genomic and immunological traits behind mutations were elucidated. Melanoma patients with mutations had a markedly extended ICI outcome than other patients. An association between mutations and the improved outcome was further confirmed in NSCLC. In addition, an elevated ICI response rate was presented in -mutated NSCLC patients (81.8% vs. 29.7%, = 0.002). Subsequent analyses revealed that -mutated patients had a favorable abundance of response immunocytes, an inferior abundance of suppression immunocytes, enhanced mutational burden, and interferon response-relevant signaling pathways. We uncovered that mutations were predictive of a better ICI response and associated with preferable immunogenicity, which may be considered as a genomic determinant to customize biotherapy strategies.
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http://dx.doi.org/10.3390/cancers14143495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315784PMC
July 2022
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