Publications by authors named "Qiang Hu"

329 Publications

Core-shell nanostructured Zn-Co-O@CoS arrays for high-performance hybrid supercapacitors.

Dalton Trans 2021 Apr 24;50(14):4923-4931. Epub 2021 Mar 24.

Ecology and Health Institute, Hangzhou Vocational and Technical College, Hangzhou 310018, China.

Although zinc oxide (ZnO) with wide distribution is one of the most attractive energy storage materials, the low electronic conductivity and insufficient active sites of bulk ZnO increase the internal resistance and reduce the capacity of electrodes for supercapacitors. Herein, CoS nanosheets are coated on the surface of heterostructured ZnO/CoO nanowires to synthesize a core-shell Zn-Co-O@CoS electrode by a three-step method. The built-in electric field formed between ZnO and CoO can enhance the conductivity of the composite electrode. The coating of amorphous CoS can also provide sufficient active sites and improve the chemical stability of ZnO/CoO nanowires. As a result, the as-prepared Zn-Co-O@CoS electrode delivers a high specific capacity of 1190 C g, which is 7 times higher than that of the pristine ZnO electrode. Besides, a hybrid supercapacitor (HSC) with the Zn-Co-O@CoS electrode exhibits a high energy density of 56.8 W h kg at a power density of 771.6 W kg. Furthermore, we assembled a solar-charging power system by combining the HSC and monocrystalline silicon plates to prove the practicability of the device, which can power a toy electric fan successfully. This study provides an effective idea and strategy for preparing Zn-based supercapacitor electrodes with low cost and deep discharge.
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http://dx.doi.org/10.1039/d1dt00584gDOI Listing
April 2021

The preconditioning of lithium promotes mesenchymal stem cell-based therapy for the degenerated intervertebral disc via upregulating cellular ROS.

Stem Cell Res Ther 2021 Apr 14;12(1):239. Epub 2021 Apr 14.

Department of Orthopaedics, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, #88 Jiefang Road, Hangzhou, China.

Adipose-derived stem cell (ADSC) is one of the most widely used candidate cell for intervertebral disc (IVD) degeneration-related disease. However, the poor survival and low differentiation efficacy in stressed host microenvironment limit the therapeutic effects of ADSC-based therapy. The preconditioning has been found effective to boost the proliferation and the functioning of stem cells in varying pathological condition. Lithium is a common anti-depression drug and has been proved effective to enhance stem cell functioning. In this study, the effects of preconditioning using LiCl on the cellular behavior of ADSC was investigated, and specially in a degenerative IVD-like condition.

Method: The cellular toxicity on rat ADSC was assessed by detecting lactate dehydrogenase (LDH) production after treatment with a varying concentration of lithium chloride (LiCl). The proliferative capacity of ADSC was determined by detecting Ki67 expression and the relative cell number of ADSC. Then, the preconditioned ADSC was challenged by a degenerative IVD-like condition. And the cell viability as well as the nucleus pulpous (NP) cell differentiation efficacy of preconditioned ADSC was evaluated by detecting the major marker expression and extracellular matrix (ECM) deposit. The therapeutic effects of preconditioned ADSC were evaluated using an IVD degeneration rat model, and the NP morphology and ECM content were assessed.

Results: A concentration range of 1-10 mmol/L of LiCl was applied in the following study, since a higher concentration of LiCl causes a major cell death (about 40%). The relative cell number was similar between preconditioned groups and the control group after preconditioning. The Ki67 expression was elevated after preconditioning. Consistently, the preconditioned ADSC showed stronger proliferation capacity. Besides, the preconditioned groups exhibit higher expression of NP markers than the control group after NP cell induction. Moreover, the preconditioning of LiCl reduced the cell death and promoted ECM deposits, when challenged with a degenerative IVD-like culture. Mechanically, the preconditioning of LiCl induced an increased cellular reactive oxidative species (ROS) level and activation of ERK1/2, which was found closely related to the enhanced cell survival and ECM deposits after preconditioning. The treatment with preconditioned ADSC showed better therapeutic effects than control ADSC transplantation, with better NP preservation and ECM deposits.

Conclusion: These results suggest that the preconditioning with a medium level of LiCl boosts the cell proliferation and differentiation efficacy under a normal or hostile culture condition via the activation of cellular ROS/ERK axis. It is a promising pre-treatment of ADSC to promote the cell functioning and the following regenerative capacity, with superior therapeutic effects than untreated ADSC transplantation.
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http://dx.doi.org/10.1186/s13287-021-02306-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048279PMC
April 2021

A CMOS Hall sensor modeling with readout circuitry and microcontroller processing for magnetic detection.

Rev Sci Instrum 2021 Mar;92(3):034707

James Watt School of Engineering, University of Glasgow, G12 8QQ Glasgow, United Kingdom.

A Hall sensor array system for magnetic field detection and analysis is realized in X-FAB 0.18 μm CMOS technology. Magnetic field detection is attributed to the magnetization of metal coils to metal particles and the sensing characteristics of the Hall sensor array. The system puts forward a complete solution from Hall sensors, analog front-end circuit, analog-to-digital converter (ADC) to microcontroller unit. Using Ansoft Maxwell and COMSOL Multiphysics software for simulation verification, the minimum diameter of magnetic particles that can be detected in the system is 2 μm. The measured signal to noise and distortion ratio, spurious free dynamic range, and effective number of bits of the proposed ADC are 70.61 dB, 90.08 dB, and 11.44-bit, respectively. The microsystem based on STM32 combines hardware and software design, which can effectively adjust the motion parameters and realize the real-time display in the LCD screen of the magnetic field and voltage information. Compared to the prior system, the portability, cost, and efficiency have been considerably improved, which is aimed at the rapid measurement of heavy metal particles such as Fe, Co, and Ni in ambient air and blood.
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http://dx.doi.org/10.1063/5.0038295DOI Listing
March 2021

Food waste treating by biochar-assisted high-solid anaerobic digestion coupled with steam gasification: Enhanced bioenergy generation and porous biochar production.

Bioresour Technol 2021 Jul 26;331:125051. Epub 2021 Mar 26.

National Engineering Research Center for Nanotechnology, Shanghai Jiao Tong University, Shanghai 200241, PR China.

A food waste treating system was proposed in this study by combining biochar-assisted high-solid anaerobic digestion and subsequent steam gasification of the digestate. The effect of solid level, biochar dosage in anaerobic digestion on the properties of biogas, syngas, and final biochar products were investigated. Results showed that at a high total solid level and biochar dosage of 25 g/L and 50 g/L, the accumulative methane yield reached 110.3 mL CH/g VS and 126.7 mL CH/g VS, respectively. From steam gasification of different digestates under 850 °C for 15 min, a maximum of 34.92 mmol/g for the hydrogen yield and 11.44 MJ/m for the higher heating value could be obtained for the syngas. Furthermore, the by-product produced from steam gasification was a nutrient-enriched porous biochar, which was suitable to be used as compost. This study demonstrated a pathway for food waste treating to produce methane-enriched biogas, hydrogen-enriched syngas, and nutrient-enriched biochar.
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http://dx.doi.org/10.1016/j.biortech.2021.125051DOI Listing
July 2021

A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga .

Plants (Basel) 2021 Mar 31;10(4). Epub 2021 Mar 31.

Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the main constituent lipids of thylakoid and chloroplast envelop membranes. Many microalgae can accumulate large amounts of triacylglycerols (TAGs) under adverse environmental conditions, which is accompanied by degradation of the photosynthetic membrane lipids. However, the process mediating the conversion from galactolipids to TAG remains largely unknown. In this study, we performed genetic and biochemical analyses of galactosyl hydrolases (CrGH) identified in the proteome of lipid bodies of the green microalga . The recombinant CrGH was confirmed to possess galactosyl hydrolase activity by using o-nitrophenyl-β-D-galactoside as the substrate, and the Michaelis constant (Km) and Kcat of CrGH were 13.98 μM and 3.62 s, respectively. Comparative lipidomic analyses showed that the content of MGDG and DGDG increased by 14.42% and 24.88%, respectively, in the -deficient mutant as compared with that of the wild type cc4533 grown under high light stress conditions, and meanwhile, the TAG content decreased by 32.20%. Up-regulation of CrGH at both a gene expression and protein level was observed under high light stress (HL) conditions. In addition, CrGH was detected in multiple subcellular localizations, including the chloroplast envelope, mitochondria, and endoplasmic reticulum membranes. This study uncovered a new paradigm mediated by the multi-localized CrGH for the conversion of the photosynthetic membranes to TAGs.
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http://dx.doi.org/10.3390/plants10040675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065580PMC
March 2021

Comment on: Impact of anastomotic leak on long-term survival in patients undergoing gastrectomy for gastric cancer.

Br J Surg 2021 Apr;108(3):e141

Department of Traditional Chinese Medicine, Community Health Service Center of Guali Town of Xiaoshan, 180 Guayu RD, Hangzhou, 311241, China.

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http://dx.doi.org/10.1093/bjs/znaa157DOI Listing
April 2021

Bioconductor toolchain for reproducible bioinformatics pipelines using Rcwl and RcwlPipelines.

Bioinformatics 2021 Mar 27. Epub 2021 Mar 27.

Department of Biostatistics & Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY.

Summary: The Common Workflow Language (CWL) is used to provide portable and reproducible data analysis workflows across different tools and computing environments. We have developed Rcwl, an R interface to CWL, to provide easier development, use, and maintenance of CWL pipelines from within R. We have also collected more than 100 pre-built tools and pipelines in RcwlPipelines, ready to be queried and used by researchers in their own analysis. A single-cell RNA sequencing preprocessing pipeline demonstrates use of the software.

Availability: Project website: https://rcwl.org (Rcwl: https://bioconductor.org/packages/Rcwl; RcwlPipelines: https://bioconductor.org/packages/RcwlPipelines).

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab208DOI Listing
March 2021

Comment on: "Laparoscopic treatment (reTAPP) for recurrence after laparoscopic inguinal hernia repair".

Hernia 2021 Mar 25. Epub 2021 Mar 25.

Department of General Surgery, Tongde Hospital of Zhejiang Province, 234 Gucui RD, Hangzhou, 310012, China.

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http://dx.doi.org/10.1007/s10029-021-02386-9DOI Listing
March 2021

Comment on "Early postoperative outcomes among patients with delayed surgeries after preoperative positive test for SARS-CoV-2: A case-control study from a single institution".

J Surg Oncol 2021 Mar 22. Epub 2021 Mar 22.

Department of General Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, China.

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http://dx.doi.org/10.1002/jso.26414DOI Listing
March 2021

A comparison between manual and artificial intelligence-based automatic positioning in CT imaging for COVID-19 patients.

Eur Radiol 2021 Mar 19. Epub 2021 Mar 19.

Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, NO.169 Donghu Road, Wuchang District, Wuhan, 430071, Hubei Province, People's Republic of China.

Objective: To analyze and compare the imaging workflow, radiation dose, and image quality for COVID-19 patients examined using either the conventional manual positioning (MP) method or an AI-based automatic positioning (AP) method.

Materials And Methods: One hundred twenty-seven adult COVID-19 patients underwent chest CT scans on a CT scanner using the same scan protocol except with the manual positioning (MP group) for the initial scan and an AI-based automatic positioning method (AP group) for the follow-up scan. Radiation dose, patient positioning time, and off-center distance of the two groups were recorded and compared. Image noise and signal-to-noise ratio (SNR) were assessed by three experienced radiologists and were compared between the two groups.

Results: The AP operation was successful for all patients in the AP group and reduced the total positioning time by 28% compared with the MP group. Compared with the MP group, the AP group had significantly less patient off-center distance (AP 1.56 cm ± 0.83 vs. MP 4.05 cm ± 2.40, p < 0.001) and higher proportion of positioning accuracy (AP 99% vs. MP 92%), resulting in 16% radiation dose reduction (AP 6.1 mSv ± 1.3 vs. MP 7.3 mSv ± 1.2, p < 0.001) and 9% image noise reduction in erector spinae and lower noise and higher SNR for lesions in the pulmonary peripheral areas.

Conclusion: The AI-based automatic positioning and centering in CT imaging is a promising new technique for reducing radiation dose and optimizing imaging workflow and image quality in imaging the chest.

Key Points: • The AI-based automatic positioning (AP) operation was successful for all patients in our study. • AP method reduced the total positioning time by 28% compared with the manual positioning (MP). • AP method had less patient off-center distance and higher proportion of positioning accuracy than MP method, resulting in 16% radiation dose reduction and 9% image noise reduction in erector spinae.
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http://dx.doi.org/10.1007/s00330-020-07629-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975236PMC
March 2021

Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR-24-3p/LRG1 axis in retinoblastoma cells.

Exp Ther Med 2021 Apr 18;21(4):367. Epub 2021 Feb 18.

Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.

Retinoblastoma (RB) is the most common primary intraocular cancer type that occurs during retinal development in childhood. Previous studies have reported that long non-coding RNAs (lncRNAs) are involved in the development of RB. Therefore, the aim of the present study was to investigate the effects and underlying regulatory mechanisms of nuclear paraspeckle assembly transcript 1 (NEAT1) in RB. The expression levels of NEAT1, microRNA (miR)-24-3p and leucine-rich-α-2-glycoprotein (LRG1) were detected using reverse transcription-quantitative PCR (RT-qPCR). Moreover, the protein expression levels of LRG1, matrix metalloproteinase 9, N-cadherin and E-cadherin were detected via western blotting. Furthermore, cell migration and invasion abilities were evaluated via Transwell assays. The targeting relationships between miR-24-3p and NEAT1 or LRG1 were predicted using online software and confirmed via dual-luciferase reporter assay. In the present study, NEAT1 and LRG1 were upregulated, and miR-24-3p was downregulated in RB tissues and cells compared with the corresponding healthy tissues and cells. Moreover, miR-24-3p was identified as a target of NEAT and LRG1 was demonstrated to be a direct target gene of miR-24-3p. Knockdown of NEAT1 or LRG1 significantly suppressed RB cell migration and invasion ability, while the effects were reversed by an miR-24-3p inhibitor. In addition, the downregulation of LRG1 caused by miR-24-3p was restored following the overexpression of NEAT1 in RB cells. It was also demonstrated that NEAT1 knockdown inhibited the epithelial-to-mesenchymal transition (EMT) pathway by inhibiting the expression of LRG via targeting miR-24-3p. In conclusion, the present results suggest that silencing of NEAT1 suppresses cell migration, invasion and the EMT process by downregulating LRG1 expression via sponging miR-24-3p in RB, thus indicating that NEAT1 may be a potential candidate for RB treatment.
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http://dx.doi.org/10.3892/etm.2021.9798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903428PMC
April 2021

Interaction between the cell walls of microalgal host and fungal carbohydrate-activate enzymes is essential for the pathogenic parasitism process.

Environ Microbiol 2021 Mar 15. Epub 2021 Mar 15.

Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Fungi can parasitize microalgae, exerting profound impacts on both the aquatic ecosystems and microalgal mass cultures. In this study, the unicellular green alga Haematococcus pluvialis and the blastocladialean fungus Paraphysoderma sedebokerense, were used as a model system to address the mechanisms underlying the fungal parasitism on the algal host. High-throughput metabolic assay indicated that P. sedebokerense can utilize several carbon sources with a preference for mannose, glucose and their oligosaccharides, which was compatible with the profile of the host algal cell walls enriched with glucan and mannan. The results of dual transcriptomics analysis suggested that P. sedebokerense can up-regulate a large number of putative carbohydrate-activate enzymes (CAZymes) encoding genes, including those coding for the endo-1,4-β-glucanase and endo-1,4-β-mannanase during the infection process. The cell walls of H. pluvialis can be decomposed by both P. sedebokerense and commercial CAZymes (e.g. cellulase and endo-1,4-β-mannanase) to produce mannooligomers, while several putative parasitism-related genes of P. sedebokerense can be in turn up-regulated by mannooligomers. In addition, the parasitism can be blocked through interfering the selected CAZymes including glucanase, mannanase, and lysozyme with the specific inhibitors, which provided a framework for screening suitable compounds for pathogen mitigation in algal mass culture. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15465DOI Listing
March 2021

Rational design of flower-like Co-Zn LDH@Co(HPO) heterojunctions as advanced electrode materials for supercapacitors.

Dalton Trans 2021 Apr 15;50(13):4643-4650. Epub 2021 Mar 15.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610066, China.

Layered double hydroxides (LDHs) with high theoretical specific capacity have been considered as one of the most promising candidates for high-performance supercapacitors. However, the low electronic conductivity and insufficient active sites hinder the further large-scale application of bulk LDHs. Here, we successfully synthesized heterostructured Co-Zn LDH@Co(HPO) nanoflowers by a simple hydrothermal method. As the amount of Co(HPO) in the whole heterostructure increases, the nanosheets steadily evolve into nanoflowers with a high surface area, providing more electrochemically active sites. Moreover, the built-in electric field formed between Co-Zn LDH and Co(HPO) improves the conductivity of the composite electrode. As a result, the as-prepared Co-Zn LDH@Co(HPO) shows a high specific capacity of 919 C g at a current density of 1 A g. A hybrid supercapacitor (HSC) with activated carbon (AC) as the negative electrode and Co-Zn LDH@Co(HPO) as the positive electrode delivers an energy density of 30.4 W h kg at a power density of 400 W kg, and 95.3% of the initial capacity is retained after 5000 cycles. This study provides a novel synthesis strategy for constructing heterojunctions to enhance the energy storage properties of LDH-based materials.
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http://dx.doi.org/10.1039/d0dt03966gDOI Listing
April 2021

On the Quasi-Three Dimensional Configuration of Magnetic Clouds.

Geophys Res Lett 2021 Jan 21;48(2):e2020GL090630. Epub 2021 Jan 21.

Physics Department Montana State University Bozeman MT USA.

We develop an optimization approach to model the magnetic field configuration of magnetic clouds, based on a linear force-free formulation in three dimensions. Such a solution, dubbed the Freidberg solution, is kin to the axisymmetric Lundquist solution, but with more general "helical symmetry." The merit of our approach is demonstrated via its application to two case studies of in situ measured magnetic clouds. Both yield results of reduced  ≈ 1. Case 1 shows a winding flux rope configuration with one major polarity. Case 2 exhibits a double-helix configuration with two flux bundles winding around each other and rooted on regions of mixed polarities. This study demonstrates the three-dimensional complexity of the magnetic cloud structures.
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http://dx.doi.org/10.1029/2020GL090630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900963PMC
January 2021

Outcomes from adult veno-arterial extracorporeal membrane oxygenation in a cardiovascular disease center from 2009 to 2019.

Perfusion 2021 Feb 15:267659121993365. Epub 2021 Feb 15.

Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Fuwai Hospital, Beijing, China.

Introduction: Extracorporeal membrane oxygenation (ECMO) is an imperative short-term cardiopulmonary support device now. We aimed to provide a single-center experience of veno-arterial (V-A) ECMO management and identify the risk factors of in-hospital mortality.

Methods: We conducted a retrospective review of adult patients who received V-A ECMO between 2009 and 2019 in a cardiovascular disease center. The risk factor analysis of in-hospital mortality was conducted.

Results: The study reviewed 236 patients, with an overall survival rate of 68.2%. The survivors' blood lactate concentration is significantly lower than non-survivors [7.4 (7.8) vs 11.1 (9.7),  = 0.002]. Patients who received heart transplantation were with higher in-hospital survival rate. Survivors developed less hepatic dysfunction, acute kidney injury and myocardial damage [23 (14.3%) vs 19 (25.3%),  = 0.039; 81 (50.3%) vs 51 (68%),  = 0.011; 24 (14.9%) vs 22 (29.3%),  = 0.009, respectively], with higher rate of continuous renal replacement therapy (CRRT) [56 (34.8%) vs 53 (70.7%),  < 0.001]. Fewer survivors' 24 hours and total chest drainage was over 1000 mL, and the rate of re-exploration as well as red blood cell and platelet transfusion were lower in survivors. In multivariate analysis, female, pre-ECMO blood lactate concentration, hyperlipidemia, CRRT, and 24 hours chest drainage ⩾ 1000 mL were risk factors of early mortality.

Conclusions: By providing a general description of V-A ECMO practice at a single-center in China. Post-heart transplant graft failure was associated with numerically, the greatest survival in our practice. Furthermore, female sex, pre-ECMO blood lactate concentration, hyperlipidemia, CRRT, and high blood loss in chest drains are predictors of mortality in patients who undergo V-A ECMO.
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http://dx.doi.org/10.1177/0267659121993365DOI Listing
February 2021

Comment on "post-discharge oral nutritional supplements with dietary advice in patients at nutritional risk after surgery for gastric cancer: A randomized clinical trial".

Clin Nutr 2021 Mar 29;40(3):1438. Epub 2021 Jan 29.

Department of General Surgery, Tongde Hospital of Zhejiang Province, 234 Gucui RD, Hangzhou, 310012, China. Electronic address:

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http://dx.doi.org/10.1016/j.clnu.2021.01.034DOI Listing
March 2021

Assessment of a novel oleaginous filamentous microalga Klebsormidium sp. Lgx80 (Streptophyta, Klebsormidiales) for biomass and lipid production.

J Phycol 2021 Feb 2. Epub 2021 Feb 2.

SDIC Microalgae Biotechnology Center, SDIC Biotech Investment Co. LTD, Beijing, 100000, China.

Commercial cultivation of eukaryotic microalgae has so far employed a unicellular form of species only (e.g., Chlorella pyrenoidosa, Dunaliella salina and Haematococcus pluvialis). In this study, we assessed the feasibility of using the filamentous eukaryotic microalga Klebsormidium sp. LGX80 as a new cultivar for biomass and lipid production. The effects of different forms and concentrations of nitrogen on growth and lipid production of Klebsormidium sp. LGX80 were studied by using a glass column (ø4.5×60 cm) photobioreactor under laboratory conditions. Growth and lipid production of the new strain were further evaluated in a pilot-scale tubular photobioreactor outdoors. The results showed that when supplied with urea as a source of nitrogen Klebsormidium sp. LGX80 yielded a final biomass concentration of 8.49±0.10 g · L in which a cellular lipid content of was 59.2±0.4% DW. Under such conditions, the biomass and lipid productivities were 471.7±5.9 and 248.1±0.0 mg · L · d , respectively. Fatty acid analysis revealed that the main fatty acids of Klebsormidium sp. LGX80 were palmitic acid (C16:0), linoleic acid (C18:2ω6) and linolenic acid (C18:3ω3), of which linoleic acid (C18:2ω6) accounted for up to 67.5±0.1% of total fatty acids. When grown in a 13,000 liter tubular photobioreactor outdoors with an initial nitrogen concentration of 3 mM urea, Klebsormidium sp. LGX80 reached the highest biomass concentration of 2.63±0.09 g · L with the cells containing 38.0±0.5% lipids (% DW), resulting in the volumetric biomass and lipid productivities of 147.2±3.6 and 37.9±0.9 mg · L d respectively. The results of light:dark cycle experiment showed that a durative and prolonged light irradiation hindered the biosynthesis of chlorophyll a and chlorophyll b in the cells, but promoted the carotenoid accumulation. These results suggested that Klebsormidium sp. LGX80 can be a potential oleaginous filamentous microalga for commercial production of microalgal oils.
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http://dx.doi.org/10.1111/jpy.13137DOI Listing
February 2021

Ultradeep sequencing differentiates patterns of skin clonal mutations associated with sun-exposure status and skin cancer burden.

Sci Adv 2021 Jan 1;7(1). Epub 2021 Jan 1.

Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.

In ultraviolet (UV) radiation-exposed skin, mutations fuel clonal cell growth. The relationship between UV exposure and the accumulation of clonal mutations (CMs) and the correlation between CMs and skin cancer risk are largely unexplored. We characterized 450 individual-matched sun-exposed (SE) and non-SE (NE) normal human skin samples. The number and relative contribution of CMs were significantly different between SE and NE areas. Furthermore, we identified hotspots in , , and where mutations were significantly associated with UV exposure. In the normal skin from patients with cutaneous squamous cell carcinoma, we found that the cancer burden was associated with the UV-induced mutations, with the difference mostly conferred by the low-frequency CMs. These findings provide previously unknown information on UV's carcinogenic effect and pave the road for future development of quantitative assessment of subclinical UV damage and skin cancer risk.
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http://dx.doi.org/10.1126/sciadv.abd7703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775785PMC
January 2021

MUC1-C integrates activation of the IFN-γ pathway with suppression of the tumor immune microenvironment in triple-negative breast cancer.

J Immunother Cancer 2021 Jan;9(1)

Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA

Background: Immune checkpoint inhibitors (ICIs) have had a profound impact on the treatment of many tumors; however, their effectiveness against triple-negative breast cancers (TNBCs) has been limited. One factor limiting responsiveness of TNBCs to ICIs is a lack of functional tumor-infiltrating lymphocytes (TILs) in 'non-inflamed' or 'cold' tumor immune microenvironments (TIMEs), although by unknown mechanisms. Targeting MUC1-C in a mouse transgenic TNBC tumor model increases cytotoxic tumor-infiltrating CD8+ T cells (CTLs), supporting a role for MUC1-C in immune evasion. The basis for these findings and whether they extend to human TNBCs are not known.

Methods: Human TNBC cells silenced for MUC1-C using short hairpin RNAs (shRNAs) were analyzed for the effects of MUC1-C on global transcriptional profiles. Differential expression and rank order analysis was used for gene set enrichment analysis (GSEA). Gene expression was confirmed by quantitative reverse-transcription PCR and immunoblotting. The The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA) and Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) datasets were analyzed for effects of MUC1 on GSEA, cell-type enrichment, and tumor immune dysfunction and exclusion. Single-cell scRNA-seq datasets of TNBC samples were analyzed for normalized expression associations between MUC1 and selected genes within tumor cells.

Results: Our results demonstrate that MUC1-C is a master regulator of the TNBC transcriptome and that MUC1-C-induced gene expression is driven by STAT1 and IRF1. We found that MUC1-C activates the inflammatory interferon (IFN)-γ-driven JAK1→STAT1→IRF1 pathway and induces the IDO1 and COX2/PTGS2 effectors, which play key roles in immunosuppression. Involvement of MUC1-C in activating the immunosuppressive IFN-γ pathway was extended by analysis of human bulk and scRNA-seq datasets. We further demonstrate that MUC1 associates with the depletion and dysfunction of CD8+ T cells in the TNBC TIME.

Conclusions: These findings demonstrate that MUC1-C integrates activation of the immunosuppressive IFN-γ pathway with depletion of TILs in the TNBC TIME and provide support for MUC1-C as a potential target for improving TNBC treatment alone and in combination with ICIs. Of translational significance, MUC1-C is a druggable target with chimeric antigen receptor (CAR) T cells, antibody-drug conjugates (ADCs) and a functional inhibitor that are under clinical development.
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http://dx.doi.org/10.1136/jitc-2020-002115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839859PMC
January 2021

Oliceridine for the Management of Acute Postoperative Pain.

Ann Pharmacother 2021 Jan 11:1060028020987679. Epub 2021 Jan 11.

Linyi Central Hospital, Shandong, China.

Objective: To review the pharmacological characteristics, clinical evidence, and place in the management of acute postoperative pain severe enough to require an intravenous opioid.

Data Sources: A comprehensive literature search was conducted in PubMed (January 2000 to December 1, 2020). Key search terms included or . Other sources were derived from product labeling and ClinicalTrials.gov.

Study Selection And Data Extraction: All English-language articles identified from the data sources were reviewed and evaluated. Phase I, II, and III clinical trials were included.

Data Synthesis: Oliceridine is a novel selective µ-receptor G-protein pathway modulator. It has the property of activating G-protein signaling while causing low β-arrestin recruitment to the µ-receptor. Intravenous oliceridine showed statistically superior analgesia than placebo in patients with moderate or severe pain after surgery, with a favorable safety and tolerability profile regarding respiratory and gastrointestinal adverse effects, compared with morphine.

Relevance To Patient Care And Clinical Practice: The analgesic capacity of oliceridine is at least comparable to that of morphine at clinically relevant dosages, with a rapid onset of action. Also, it may be associated with a lower incidence of adverse events at dosing regimens associated with comparable analgesia. These data suggest that oliceridine may provide an important new treatment option for the management of moderate to severe postoperative pain where an intravenous opioid is warranted.

Conclusion: Oliceridine has obvious analgesic effects in patients with moderate or severe pain after surgery; additionally, it has a favorable safety and tolerability profile.
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http://dx.doi.org/10.1177/1060028020987679DOI Listing
January 2021

Recurrent thrombosis in the lower extremities after thrombectomy in a patient with polycythemia vera: A case report.

World J Clin Cases 2020 Dec;8(24):6473-6479

Department of Vascular Surgery, People's Hospital of Quzhou, Quzhou 324000, Zhejiang Province, China.

Background: Acute arterial embolism of the extremities is a surgical emergency. Atrial fibrillation is the major etiology of acute arterial embolism of the extremities. Emergency femoral artery thrombectomy can successfully treat this issue. However, polycythemia vera (PV) may sometimes explain this medical emergency. Recurrent thrombosis in the lower extremities after thrombectomy can be found in patients with PV, and reoperation is needed for this condition.

Case Summary: A 68-year-old man in China suffered from sudden pain in the left lower extremity for 14 h. The examination in the emergency department showed a diagnosis of acute arterial embolism of the extremities combined with PV. The patient's complaint disappeared after repeat emergency thrombectomy.

Conclusion: Patients with acute arterial embolism of the extremities should be treated carefully, especially those who have recurrent thrombosis after emergency thrombectomy. Clinicians should be aware of PV, a rare cause of acute arterial embolism of the extremities. The combination of thrombectomy, phlebotomy, and antiplatelet and anticoagulant drugs may be a suitable therapeutic regimen for these patients.
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http://dx.doi.org/10.12998/wjcc.v8.i24.6473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760424PMC
December 2020

Comment on "The impact of sarcopenia on oncologic abdominal wall reconstruction".

J Surg Oncol 2021 02 23;123(2):694-695. Epub 2020 Dec 23.

Department of Traditional Chinese Medicine, Community Health Service Center of Guali Town of Xiaoshan, Hangzhou, China.

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http://dx.doi.org/10.1002/jso.26219DOI Listing
February 2021

Research on the NIQAS device for hazardous goods identification based on PGNAA technology.

Appl Radiat Isot 2021 Mar 15;169:109445. Epub 2020 Dec 15.

Institute of Nuclear Analytical Technology, College of Materials Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China.

In the research, the Nuclide Identification and Quantitative Analysis System (NIQAS) for the identification of hazardous goods was designed and optimized by MCNP simulations. With the D-T neutron generator and HPGe detector set as the core of device, the modules, including reflector, moderator and shielding, etc., were optimized by a Signal-to-Noise Ratio (SNR) evaluation method. Moreover, the NIQAS device was set up for measuring the hazardous goods, and the type identification was discussed based on the established logical decision. Finally, a Graphical User Interface (GUI) was designed and the minimum detection time was further investigated.
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http://dx.doi.org/10.1016/j.apradiso.2020.109445DOI Listing
March 2021

Diversity in Androgen Receptor Action Among Treatment-naïve Prostate Cancers Is Reflected in Treatment Response Predictions and Molecular Subtypes.

Eur Urol Open Sci 2020 Dec 6;22:34-44. Epub 2020 Nov 6.

Department of Cancer Biology, Cleveland Clinic, Cleveland, OH, USA.

Background: Metastatic prostate cancer (CaP) treatments are evolving rapidly but without evidence-based biomarkers to predict responses, and to maximize remissions and survival.

Objective: To determine the activity of androgen receptor (AR), the target for default first-line systemic treatment, in localized treatment-naïve CaP and its association with clinical risk factors, molecular markers, CaP subtypes, and predictors of treatment response.

Design Setting And Participants: We examined 452 bona fide AR target genes in clinical-grade expression profiles from 6532 such CaPs collected between 2013 and 2017 by US physicians ordering the Decipher RP test. Results were validated in three independent smaller cohorts ( = 73, 90, and 127) and clinical CaP AR ChIP-Seq data. Association with CaP differentiation and progression was analyzed in independent datasets.

Outcome Measurements And Statistical Analysis: Unsupervised clustering of CaPs based on AR target gene expression was aligned with clinical variables, differentiation scores, molecular subtypes, and predictors of response to hormonal therapy, radiotherapy, and chemotherapy. AR target gene sets were analyzed via Gene Set Enrichment Analysis for differentiation and treatment resistance, Ingenuity Pathway Analysis for associated biology, and Cistrome for genomic AR binding site (ARBS) composition.

Results And Limitations: Expression of eight AR target gene subsignatures gave rise to five CaP clusters, which were preferentially associated with CaP molecular subtypes, differentiation, and predictors of treatment response rather than with clinical variables. Subsignatures differed in contribution to CaP progression, luminal/basal differentiation, CaP biology, and ARBS composition. Validation in prospective trials and optimized quantitation are needed for clinical implementation.

Conclusions: Measurement of AR activity patterns in treatment-naïve CaP may serve as a first branch of an evidence-based decision tree to optimize personalized treatment plans.

Patient Summary: Treatment options for metastatic prostate cancer are increasing without information needed to choose the right treatment for the right patient. We found variation in the behavior of the target for the default first-line therapy before treatment, which may help optimize treatment plans.
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http://dx.doi.org/10.1016/j.euros.2020.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723342PMC
December 2020

Experimental Study on the Explosion Characteristics of CH/O/N Mixtures with Different Oxygen Enrichment Coefficients and Ignition Positions.

ACS Omega 2020 Dec 17;5(47):30495-30501. Epub 2020 Nov 17.

School of Mechanical and Power Engineering, Henan Polytechnic University, Jiaozuo 454003, PR China.

In this paper, the flame propagation characteristics and overpressure oscillation characteristics of CH explosion were studied under different ignition positions (IPs) and oxygen enrichment conditions in a half-open tube. The distances between the IP and the closed end of the tube are 0, 250, 500, and 750 mm. The oxygen enrichment coefficient (φ) values used in the experiment are 0.21, 0.3, and 0.4. The experimental results show that the IP and oxygen enrichment coefficient have an important influence on the flame structure and overpressure oscillation. Only when the oxygen enrichment coefficient φ = 0.21, a tulip flame will be formed. The IP close to the outlet can make the air participate in the combustion more quickly. With the increase of the oxygen enrichment coefficient, the combustion-induced rapid phase transition phenomenon is more likely to occur, and the maximum overpressure value and the overpressure rise rate of flame will increase. It is worth noting that after increasing the oxygen enrichment coefficient, the IP has less influence than the oxygen enrichment coefficient on the overpressure rise rate.
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http://dx.doi.org/10.1021/acsomega.0c04346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711701PMC
December 2020

Biochar industry to circular economy.

Sci Total Environ 2021 Feb 19;757:143820. Epub 2020 Nov 19.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore. Electronic address:

Biochar, produced as a by-product of pyrolysis/gasification of waste biomass, shows great potential to reduce the environment impact, address the climate change issue, and establish a circular economy model. Despite the promising outlook, the research on the benefits of biochar remains highly debated. This has been attributed to the heterogeneity of biochar itself, with its inherent physical, chemical and biological properties highly influenced by production variables such as feedstock types and treating conditions. Hence, to enable meaningful comparison of results, establishment of an agreed international standard to govern the production of biochar for specific uses is necessary. In this study, we analyzed four key uses of biochar: 1) in agriculture and horticulture, 2) as construction material, 3) as activated carbon, and 4) in anaerobic digestion. Then the guidelines for the properties of biochar, especially for the concentrations of toxic heavy metals, for its environmental friendly application were proposed in the context of Singapore. The international status of the biochar industry code of practice, feedback from Singapore local industry and government agencies, as well as future perspectives for the biochar industry were explained.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143820DOI Listing
February 2021

[Effects of substrate on growth and lipid accumulation of Tribonema sp. FACHB-1786].

Sheng Wu Gong Cheng Xue Bao 2020 Nov;36(11):2478-2493

Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China.

Filamentous microalga Tribonema sp. has the advantages of highly resistance to zooplankton-predation, easy harvesting, and high cellular lipid content, in particular large amounts of palmitoleic acid (PA) and eicosapentaenoic acid (EPA). Therefore, Tribonema sp. is considered as a promising biomass feedstock to produce biodiesel and high-value products. In this work, we studied the effect of different concentrations of nitrogen (NaNO₃: 255-3 060 mg/L), phosphorus (K₂HPO₄: 4-240 mg/L), iron ((NH₄)₃FeC₁₂H₁₀O₁₄: 0.6-12 mg/L) and magnesium (MgSO₄: 7.5-450 mg/L) on the biomass, lipid content, and fatty acid composition of Tribonema sp. FACHB-1786, aiming at enhancing cell lipid productivity. The growth of Tribonema sp. had a positive correlation with the concentration of magnesium, and the maximum biomass of Tribonema sp. (under the condition of 450 mg/L MgSO₄) was 8.09 g/L, much greater than those reported in previous studies using the same and other Tribonema species under autotrophic conditions. Different nitrogen concentrations exerted no significant effect on algal growth (P > 0.05), but a higher nitrogen concentration resulted in a greater amount of lipid in the cells. The maximum volumetric productivities of total lipids (319. 6 mg/(L·d)), palmitoleic acid (135.7 mg/(L·d)), and eicosapentaenoic acid (24.2 mg/(L·d)) of Tribonema sp. were obtained when the concentrations of NaNO₃, K₂HPO₄, (NH₄)₃FeC₁₂H₁₀O₁₄, and MgSO₄ were 765 mg/L, 80 mg/L, 6 mg/L, and 75 mg/L, respectively. This study will provide a reference for substrate optimization for Tribonema sp. growth and lipid production.
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http://dx.doi.org/10.13345/j.cjb.200264DOI Listing
November 2020

Mechanical Bowel Preparation for Colorectal Surgery: Is It Necessary?

Dis Colon Rectum 2020 09;63(9):e511

Department of General Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, China.

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http://dx.doi.org/10.1097/DCR.0000000000001607DOI Listing
September 2020

The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.

Carbohydr Polym 2021 Jan 28;252:117137. Epub 2020 Sep 28.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan, 430070, PR China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, 430070, PR China. Electronic address:

Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives. BC and PHB can be combined into a composite with enhanced mechanical properties. The synthesis processes of BC/PHB composites described until now are complicated with multiple steps. Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ. This co-culture approach ensured a certain level of control over the synthesis process. By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %. The fabricated composites were networks of BC fibers connecting PHB particles. BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC. The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
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http://dx.doi.org/10.1016/j.carbpol.2020.117137DOI Listing
January 2021

Effects of Liposomal Simvastatin Nanoparticles on Vascular Endothelial Function and Arterial Smooth Muscle Cell Apoptosis in Rats with Arteriosclerotic Occlusive Disease of Lower Limb via P38 Mitogen-Activated Protein Kinase Nuclear Factor Kappa-B Pathway.

J Nanosci Nanotechnol 2021 Feb;21(2):1169-1175

Department of Vascular Surgery, Quzhou People's Hospital, Quzhou City, 324000, Zhejiang Province, China.

This article prepared a simvastatin-NLCs for the treatment of arteriosclerotic occlusive disease of lower limbs. Taking the size distribution, polydispersity coefficient, encapsulation efficiency and drug loading of simvastatin-NLCs as evaluation indicators, various prescription factors of simvastatin- NLCs were investigated. The release behavior and stability of simvastatin-NLCs were also investigated. A hyperlipidemia rat model was established using high-fat diets. SD rats fed ordinary diet were set as normal control groups. 20 rats, 20 in the simvastatin group and 20 in the simvastatin nanocarrier group. After 5 weeks of drug intervention, the rats were sacrificed and the aorta was taken to determine the smooth muscle cell apoptosis rate. Studies have shown that simvastatin nanocarriers can more effectively reduce blood lipids in hyperlipidemia rats, increase the rate of smooth muscle cell apoptosis in hyperlipidemia rats, and delay the onset of atherosclerosis.
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http://dx.doi.org/10.1166/jnn.2021.18632DOI Listing
February 2021