Publications by authors named "Qiang Gao"

976 Publications

Amidoxime functionalized PVDF-based chelating membranes enable synchronous elimination of heavy metals and organic contaminants from wastewater.

J Environ Manage 2022 Sep 2;318:115643. Epub 2022 Jul 2.

School of Textile and Material Engineering, Dalian Polytechnic University, Dalian, China.

Aiming at the synchronous elimination of heavy metals and organic contaminants from wastewater, the amidoxime functionalized PVDF-based chelating membrane was fabricated in this study. The structure and morphology of the chelating membrane were characterized using infrared spectroscopy (FT-IR), nuclear magnetic resonance spectrometer (NMR) and scanning electron microscopy (SEM). The SEM results show that the chemical modification with amidoxime groups did not damage the structure of the PVDF-based membrane. The chelating membrane has a high removal efficiency for Cu (77.33%) and Pb (79.23%) owing to the chemisorption through coordination bonds. However, the chelating membrane exhibits a low removal efficiency for Cd (29.88%) due to the physical adsorption. The chelating membrane has a high rejection efficiency of BSA (95.17%) and lysozyme (70.09%), which is attributed to the sieving effect and increased hydrophobicity. Furthermore, the membrane performance for simultaneously removing metals and proteins from simulated wastewater was examined. The interaction of metal ions with proteins (BSA and lysozyme) can enhance the ion removal efficiency of the chelated membrane, but decrease the protein rejection efficiency due to the destructive effect. The amidoxime functionalized PVDF-based chelating membrane has a high potential for application in wastewater treatment.
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http://dx.doi.org/10.1016/j.jenvman.2022.115643DOI Listing
September 2022

rTMS for poststroke pusher syndrome: study protocol for a randomised, patient-blinded controlled clinical trial.

BMJ Open 2022 Aug 10;12(8):e064905. Epub 2022 Aug 10.

Rehabilitation Medicine Center, Sichuan University West China Hospital, Chengdu, Sichuan, China

Introduction: Poststroke pusher syndrome (PS) prevalence is high. Patients with PS require longer rehabilitation with prolonged length of stay. Effective treatment of PS remains a challenge for rehabilitation professionals. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neuromodulation technique that is effective and recommended in the clinical guidelines of stroke rehabilitation. However, the role of rTMS for PS has not been examined. The study is to assess the efficacy of a specific rTMS programme for patients with PS in reducing pushing behaviour, enhancing motor recovery and improving mobility, as well as testing the safety of rTMS for patients with PS.

Methods And Analysis: A randomised, patient and assessor blinded sham-controlled trial with two parallel groups will be conducted. Thirty-four eligible patients with PS will be randomly allocated to receive either rTMS or sham rTMS for 3 weeks. The primary assessment outcome is the pushing behaviour measured by the Burke Lateropulsion Scale and Scale for Contraversive Pushing. The secondary outcomes are the motor functions and mobility measured by the Fugl-Meyer Assessment Scale (motor domain) and Modified Rivermead Mobility Index, and any adverse events. Assessment will be performed at baseline and 1 week, 2 weeks and 3 weeks after intervention. Repeated-measures analysis of variance will be used for data analysis with the level of significance level set at 0.05.

Ethics And Dissemination: The protocol has been approved by the Biomedical Ethics Committee of West China Hospital, Sichuan University on 23 March 2022 (2022-133). The trial findings will be published in peer-reviewed journals.

Trial Registration Number: Chinese Clinical Trial Registry (ChiCTR2200058015).
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http://dx.doi.org/10.1136/bmjopen-2022-064905DOI Listing
August 2022

Insights Into miRNA-mRNA Regulatory Mechanisms of Cold Adaptation in : Ubiquitin-Mediated Proteolysis Is Pivotal for Adaptive Energy Metabolism.

Front Genet 2022 22;13:903995. Epub 2022 Jul 22.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

This study aimed to understand cold stress adaptations mechanism in fish. Thus, the transcriptional response to cold conditions in was evaluated using RNA-seq and microRNA (miRNA)-seq analyses. Low-temperature (LT) group was cultivated outdoors in waters cooled to 2-4°C for 3 weeks, while individuals in the control temperature (CT) group were exposed to 14-16°C. Significantly different responses were observed in both mRNA and miRNA expression profiles, with more mRNAs (1,833 and 1,869 mRNAs were up- and downregulated, respectively) and fewer miRNAs (15 and 6 were up- and downregulated, respectively) observed in the LT group individuals relative to the CT group individuals. A miRNA-mRNA network involved in the regulation of responses to cold stress was constructed; this network included ubiquitin-mediated proteolysis, protein processing, and oxidative phosphorylation. These results provided new insights into mechanisms of cold tolerance by fish, including decreased metabolic activity in addition to proteolysis.
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http://dx.doi.org/10.3389/fgene.2022.903995DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354851PMC
July 2022

Recent progress on covalent organic framework materials as CO reduction electrocatalysts.

Front Chem 2022 22;10:942492. Epub 2022 Jul 22.

Jiangsu Engineering and Technology Research Center of VOCs Treatment, Environmental Engineering College, Nanjing Polytechnic Institute, Nanjing, JS, China.

CO emission caused by fuel combustion and human activity has caused severe climate change and other subsequent pollutions around the world. Carbon neutralization various novel technologies to alleviate the CO level in the atmosphere has thus become one of the major topics in modern research field. These advanced technologies cover CO capture, storage and conversion, ., and electrocatalytic CO reduction reaction (CORR) by heterogeneous catalysts is among the most promising methods since it could utilize renewable energy and generate valuable fuels and chemicals. Covalent organic frameworks (COFs) represent crystalline organic polymers with highly rigid, conjugated structures and tunable porosity, which exhibit significant potential as heterogeneous electrocatalysts for CORR. This review briefly introduces related pioneering works in COF-based materials for electrocatalytic CORR in recent years and provides a basis for future design and synthesis of highly active and selective COF-based electrocatalysts in this direction.
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http://dx.doi.org/10.3389/fchem.2022.942492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355711PMC
July 2022

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks.

Front Bioeng Biotechnol 2022 19;10:966598. Epub 2022 Jul 19.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

With the rapid development of synthetic biology, a variety of biopolymers can be obtained by recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one with promising material properties, such as biodegradability and biocompatibility against the petrol-based plastics. This study reviews the recent studies focusing on the microbial synthesis of PHA, including chassis engineering, pathways engineering for various substrates utilization and PHA monomer synthesis, and PHA synthase modification. In particular, advances in metabolic engineering of dominant workhorses, for example and with outstanding PHA accumulation capability, were summarized and discussed, providing a full landscape of diverse PHA biosynthesis. Meanwhile, we also introduced the recent efforts focusing on structural analysis and mutagenesis of PHA synthase, which significantly determines the polymerization activity of varied monomer structures and PHA molecular weight. Besides, perspectives and solutions were thus proposed for achieving scale-up PHA of low cost with customized material property in the coming future.
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http://dx.doi.org/10.3389/fbioe.2022.966598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343942PMC
July 2022

Enhancement of LncRNA-HFRL expression induces cardiomyocyte inflammation, proliferation, and fibrosis via the sequestering of miR-149-5p-mediated collagen 22A inhibition.

Ann Transl Med 2022 May;10(9):523

Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Long non-coding ribonucleic acids (lncRNAs) are believed to play crucial roles in cardiovascular diseases; however, details of the underlying mechanisms by which this occurs remain unclear.

Methods: A mouse heart failure (HF) model was established using isoproterenol (ISO), and confirmed by immunostaining and echocardiography. RNA-sequencing was performed to screen the differential lncRNA expression profiles and heart failure relative lncRNA (HFRL) was selected as the target which was validated by quantitative real-time polymerase chain reaction (qRT-PCR). In HL-1 cells, the cardiac function, inflammatory, and fibrosis-related genes expression changes were examined by qRT-PCR after silencing of HFRL by lentivirus. Meanwhile, Cell Counting Kit-8 (CCK-8) assays were used to detect the effects of HFRL on the cell proliferation and viability. Reactive oxygen species (ROS) assays were also used to explore the role of HFRL in oxidative damage. Next, bioinformatics analysis was conducted to predict the potential binding microRNAs (mmu-miR-149-5p) to HFRL, which was confirmed by RNA-pulldown assays. The target gene of miR-149-5p was also predicted and further validated by Dual-luciferase reporter assays, qRT-PCR, and western blot. To investigate the synergistic regulatory effect of HFRL and miR-149-5p, HL-1 cells were infected with the lentivirus of HFRL with or without simultaneous knockdown of miR-149-5p. Then, qRT-PCR and western blot were used to examine cardiac function, inflammatory, and fibrosis-related gene expression changes, respectively. In HL-1 cells, CCK-8 assays were performed to detect the proliferation and viability. ROS assays were used to explore the oxidative damage.

Results: The administration of ISO induced mice fibrosis, inflammation, and HF. The results showed that knockdown of HFRL suppressed cardiomyocyte proliferation and viability, attenuated inflammatory, cardiac function, and fibrosis-related gene expression, and promoted oxidative damage. HFRL was found to bind to mmu-miR-149-5p and inversely target the 3'-untranscripted region of the collagen gene. Thus, HFRL affected cardiomyocyte inflammation, proliferation, viability, oxidative damage, and pro-fibrotic function via sequestration to miR-149-5p.

Conclusions: The HFRL/miR-149-5p axis plays an important role in regulating cardiac inflammation, proliferation, and fibrosis via a synergistic effect, which suggests that HFRL might be a novel target for HF.
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http://dx.doi.org/10.21037/atm-22-1756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347043PMC
May 2022

FGL2-MCOLN3-autophagy axis-triggered neutrophil extracellular traps exacerbate liver injury in fulminant viral hepatitis.

Cell Mol Gastroenterol Hepatol 2022 Aug 1. Epub 2022 Aug 1.

Department and institute of infectious diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; National Medical Center for Major Public Health Events, Wuhan, China; State Key Laboratory for Zoonotic Diseases. Electronic address:

Background & Aims: Fulminant viral hepatitis (FVH) is a life-threatening disease, but its pathogenesis is not fully understood. Neutrophil extracellular traps (NETs) were an unrecognized link between inflammation and coagulation, which are two main features of FVH. Here, we aim to investigate the role and mechanism of NETs in the pathogenesis of FVH.

Methods: A mice model of FVH was established by murine hepatitis virus strain-3 (MHV-3) infection. Liver leukocytes of infected or uninfected mice were used for single cell RNA sequencing and whole transcriptome sequencing. NETs depletion was achieved using DNase1. Acetaminophen was used to establish a mice model of non-virus caused acute liver failure. Clinically, NETs-related markers in liver, plasma and peripheral neutrophils were assessed in patients with HBV-related acute liver injury (HBV-ALI).

Results: Increased hepatic NETs formation was observed in MHV-3-infected mice but not in acetaminophen-treated mice. NETs depletion improved the liver damage and survival rate in FVH by inhibiting hepatic fibrin deposition and inflammation. Adoptive transfer experiment showed that neutrophil-specific FGL2 promoted NETs formation. FGL2 was found to directly interact with mucolipin3 (MCOLN3), which regulated calcium influx and initiated autophagy, leading to NETs formation. Clinically, elevated plasma NETs level was associated with coagulation dysfunction in patients with HBV-ALI. Colocalization of FGL2, NETs and fibrin in liver was observed in these patients.

Conclusion: NETs aggravated liver injury in FVH by promoting fibrin deposition and inflammation. NETs formation was regulated by the FGL2-MCOLN3-autophagy axis. Targeting NETs may provide a new strategy for the treatment of FVH.
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http://dx.doi.org/10.1016/j.jcmgh.2022.07.014DOI Listing
August 2022

PHD3 inhibits cell proliferation through hydroxylation of PAX2 at proline 9.

Acta Biochim Biophys Sin (Shanghai) 2022 May;54(5):708-715

Cancer Institute, the Affiliated Hospital of Qingdao University, School of Basic Medicine of Qingdao University, Qingdao 266061, China.

The oncoprotein transcription factor paired box 2 (PAX2) is aberrantly expressed in cancers, but the underlying mechanism remains elusive. Prolyl hydroxylase 3 (PHD3) hydroxylates the proline residue of HIFα, mediating HIFα degradation. The von Hippel-Lindau protein (pVHL) is an E3 ligase which mediates ubiquitination and degradation of hydroxylated HIFα. PHD3 and pVHL are found to inhibit the expression of PAX2, however, the molecular mechanism is unclear. Here we demonstrate that PHD3 hydroxylates PAX2 at proline 9, which is required for pVHL to mediate PAX2 ubiquitination and degradation. Overexpression of PHD3 enhances prolyl hydroxylation, ubiquitination and degradation of PAX2 with little effect on those of PAX2(P9A). PHD3 does not influence PAX2 expression in -null cells. pVHL binds to PAX2 and enhances PAX2 ubiquitination and degradation. However, pVHL does not bind with PAX2(P9A) and cannot enhance its ubiquitination and degradation. Our results suggest that proline 9 hydroxylation is a prerequisite for PAX2 degradation by pVHL. Functional studies indicate that introduction of PAX2 into -null COS-7 cells promotes cell proliferation, which is suppressed by co-expression of PHD3 but not by hydroxylase-deficient PHD3(H196A). PHD3 inhibits PAX2-induced, but not PAX2(P9A)-induced proliferation of COS-7 cells. These results suggest that PHD3 hydroxylates PAX2, followed by pVHL-mediated PAX2 ubiquitination and degradation. This study also suggests that PHD3 inhibits cell proliferation through downregulating PAX2.
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http://dx.doi.org/10.3724/abbs.2022043DOI Listing
May 2022

Chromosome-level assembly of Gymnocypris eckloni genome.

Sci Data 2022 08 2;9(1):464. Epub 2022 Aug 2.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China.

Gymnocypris eckloni is widely distributed in isolated lakes and the upper reaches of the Yellow River and play significant roles in the trophic web of freshwater communities. In this study, we generated a chromosome-level genome of G. eckloni using PacBio, Illumina and Hi-C sequencing data. The genome consists of 23 pseudo-chromosomes that contain 918.68 Mb of sequence, with a scaffold N50 length of 43.54 Mb. In total, 23,157 genes were annotated, representing 94.80% of the total predicted protein-coding genes. The phylogenetic analysis showed that G. eckloni was most closely related to C. carpio with an estimated divergence time of ~34.8 million years ago. For G. eckloni, we identified a high-quality genome at the chromosome level. This genome will serve as a valuable genomic resource for future research on the evolution and ecology of the schizothoracine fish in the Qinghai-Tibetan Plateau.
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http://dx.doi.org/10.1038/s41597-022-01595-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346132PMC
August 2022

The genome of Eustoma grandiflorum reveals the whole-genome triplication event contributing to ornamental traits in cultivated lisianthus.

Plant Biotechnol J 2022 Jul 25. Epub 2022 Jul 25.

Floriculture Research Institute, Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Yunnan, 650205, Kunming, China.

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http://dx.doi.org/10.1111/pbi.13899DOI Listing
July 2022

LaeA regulates morphological development and ochratoxin A biosynthesis in Aspergillus niger.

Mycotoxin Res 2022 Jul 26. Epub 2022 Jul 26.

College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

The global regulator LaeA and its orthologs govern the morphogenetic development and secondary metabolism of several filamentous ascomycetes. In Aspergillus niger, it has been shown that an LaeA ortholog (AnLaeA) regulates the production of citric acid and secondary metabolites. In this work, we constructed AnlaeA disruption and overexpression strains to investigate the roles of AnLaeA in morphological development and ochratoxin A (OTA) biosynthesis in A. niger. Phenotypic observation, chemical analysis, and gene expression analysis indicated that AnLaeA acts as a negative regulator of conidial morphogenesis and positively regulates gene expression of the OTA cluster in A. niger grown in CYA medium. However, it was observed that the upregulation of gene expression of the OTA cluster does not necessarily increase OTA production. Our results contribute to a better understanding of the AnlaeA regulatory mechanism and suggest the AnlaeA gene as a potential target for developing control strategies for A. niger infection and OTA biosynthesis.
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http://dx.doi.org/10.1007/s12550-022-00463-1DOI Listing
July 2022

Phase interface engineering of metal selenides heterostructure for enhanced lithium-ion storage and electrocatalysis.

J Colloid Interface Sci 2022 Jul 2;627:716-729. Epub 2022 Jul 2.

School of Materials Science and Engineering, Shandong University of Technology, Zibo 255000, PR China.

Biphasic or multiphase heterostructures hold attractive prospects in engineering advanced electrode materials for energy-related applications owing to the appealing synergistic effect; however, they still suffer from unsatisfied electrochemical activity and reaction kinetics. Herein, guided by density functional theory calculation, a well-engineered selenides heterostructure with high-density NiSe-NiSe biphasic interfaces that fastened in N, O-codoped carbon matrix, was developed for high-performance lithium storage and electrocatalysis. By controlled selenylation of metal-organic framework (MOF), a series of [email protected] hybrids ([email protected], NiSe/[email protected], NiSe/[email protected], and [email protected]) with tunable biphasic components and grain sizes were prepared. Abundant two-phase interfaces with higher interface density are generated inside the NiSe/NiSe-1 induced by much smaller nanograins in comparison with the NiSe/NiSe-2, so that significant charge redistribution and faster electrons/Li ions transfer kinetics are achieved within the selenides, which are proved by the mutual verification of experiment and theoretical analysis. Benefitting from this optimized heterointerfaces, the NiSe/[email protected] electrode manifests reduced polarization, superior rate capability, and prolonged cyclic stability (621.3 mAh g at 1 A g for 1000 cycles; 362.3 mAh g at 4 A g for 2000 cycles) with respect to the NiSe/[email protected], as well as excellent performance in LiCoO//NiSe/[email protected] full cell. Detailed electrochemical analysis confirmed rapid electrons/Li diffusion rates and more pseudocapacitive energy for the NiSe/[email protected] Therefore, the NiSe/[email protected] showcases superior hydrogen evolution reaction (HER) and lithium storage performance. This work demonstrates the significance of interface modulation to boost the electrochemical performance of multiphase heterostructures for energy storage and conversion.
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http://dx.doi.org/10.1016/j.jcis.2022.06.177DOI Listing
July 2022

Multimodule characterization of immune subgroups in intrahepatic cholangiocarcinoma reveals distinct therapeutic vulnerabilities.

J Immunother Cancer 2022 Jul;10(7)

Jinshan Hospital Center for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, China

Background: Immune microenvironment is well recognized as a critical regulator across cancer types, despite its complex roles in different disease conditions. Intrahepatic cholangiocarcinoma (iCCA) is characterized by a tumor-reactive milieu, emphasizing a deep insight into its immunogenomic profile to provide prognostic and therapeutic implications.

Methods: We performed genomic, transcriptomic, and proteomic characterization of 255 paired iCCA and adjacent liver tissues. We validated our findings through H&E staining (n=177), multiplex immunostaining (n=188), single-cell RNA sequencing (scRNA-seq) (n=10), functional studies, and transposon-based mouse models.

Results: Integrated multimodule data identified three immune subgroups with distinct clinical, genetic, and molecular features, designated as IG1 (immune-suppressive, 25.1%), IG2 (immune-exclusion, 42.7%), and IG3 (immune-activated, 32.2%). IG1 was characterized by excessive infiltration of neutrophils and immature dendritic cells (DCs). The hallmark of IG2 was the relatively higher tumor-proliferative activity and tumor purity. IG3 exhibited an enrichment of adaptive immune cells, natural killer cells, and activated DCs. These immune subgroups were significantly associated with prognosis and validated in two independent cohorts. Tumors with mutations were enriched in IG1 and associated with myeloid inflammation-dominated immunosuppression. Although tumor mutation burden was relatively higher in IG2, loss of heterozygosity in human leucocyte antigen and defects in antigen presentation undermined the recognition of neoantigens, contributing to immune-exclusion behavior. Pathological analysis confirmed that tumor-infiltrating lymphocytes and tertiary lymphoid structures were both predominant in IG3. Hepatitis B virus (HBV)-related samples tended to be under-represented in IG1, and scRNA-seq analyses implied that HBV infection indeed alleviated myeloid inflammation and reinvigorated antitumor immunity.

Conclusions: Our study elucidates that the immunogenomic traits of iCCA are intrinsically heterogeneous among patients, posing great challenge and opportunity for the application of personalized immunotherapy.
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http://dx.doi.org/10.1136/jitc-2022-004892DOI Listing
July 2022

The optimum parameters and neuroimaging mechanism of repetitive transcranial magnetic stimulation to post-stroke cognitive impairment, a protocol of an orthogonally-designed randomized controlled trial.

PLoS One 2022 21;17(7):e0271283. Epub 2022 Jul 21.

Department of Integrated Traditional and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Objective: Repetitive Transcranial Magnetic Stimulation (rTMS) has been used in cognition impairment due to various neuropsychiatric disorders. However, its optimum parameters and the neuroimaging mechanism are still of uncertainty. In order to simulate a study setting as close to real world as possible, the present study introduces a new orthogonally-designed protocol, consisting of the rTMS intervention with four key parameters (stimulating site, frequency, intensity and pulse number) and three different levels in each one, and aims to investigate the optimum parameters and the brain activity and connectivity in default mode network (DMN), dorsal attention network (DAN), central executive network (CEN) following rTMS intervention to post-stroke cognition impairment (PSCI).

Methods: A single-center, orthogonally-designed, triple-blind randomized controlled trial will be conducted and forty-five PSCI patients will be recruited and randomly assigned to one of nine active rTMS groups based on four rTMS paraments: stimulating site, frequency, intensity and pulse number. Neuropsychological, activities of daily living, quality of life and functional magnetic resonance imaging (fMRI) evaluations were be performed pre-, post- and 3 months after rTMS.

Discussion: This study evaluates the optimum parameters of rTMS for patients with post-stroke cognition impairment and explores the alteration of neural function in DMN, DAN, CEN brain network. These results would facilitate the standardized application of rTMS in cognition impairment rehabilitation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0271283PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302729PMC
July 2022

Geospatial immune heterogeneity reflects the diverse tumor-immune interactions in intrahepatic cholangiocarcinoma.

Cancer Discov 2022 Jul 19. Epub 2022 Jul 19.

Liver Cancer Institute, Zhong Shan Hospital and Shanghai Medical School, Fudan University,, Shanghai, China.

Intrahepatic cholangiocarcinoma (iCCA) exhibits extensive intratumoral heterogeneity and extremely high mortality rate. Here, we performed WES, RNA-seq, TCR-seq and multiplexed immunofluorescence on 207 tumor regions from 45 iCCA patients. Over half of iCCA displayed intratumoral heterogeneity of immune infiltration, and iCCA were classified into sparsely, heterogeneously, and highly infiltrated subgroups with distinct immunogenomic characteristics. Sparsely infiltrated tumors displayed active copy number loss of clonal neoantigens and heterogeneous immune infiltration played an important role in the subclonal evolution across tumor subregions. Highly infiltrated tumors were characterized by extensive immune activation and similar TCR repertoire across tumor subregions, but counteracted with T cell exhaustion and pervasive antigen presentation defects. Notably, FGFR2 mutations and fusions correlated with low mutation burden and reduced immune infiltration. Our work sculpted the dynamic tumor-immune interactions and developed a robust classification system to divide iCCA patients into high and low immune evasion groups with different prognosis.
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http://dx.doi.org/10.1158/2159-8290.CD-21-1640DOI Listing
July 2022

The "Hand as Foot" teaching method in the anatomical relationship of inferior thyroid artery and recurrent laryngeal nerve.

Asian J Surg 2022 Jul 14. Epub 2022 Jul 14.

Department of Thyroid Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, 256603, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.163DOI Listing
July 2022

The "Hand as Foot" teaching method in the anatomy of the common carotid artery, internal jugular vein and thyroid gland.

Asian J Surg 2022 Jul 14. Epub 2022 Jul 14.

Department of Thyroid Surgery, Binzhou Medical University Hospital, No. 661 Huanghe 2nd Road, Binzhou, Shandong, 256603, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.164DOI Listing
July 2022

The "Hand as Foot" teaching method in the anatomy of the thoracic duct.

Asian J Surg 2022 Jul 14. Epub 2022 Jul 14.

Department of Thyroid Surgery, Binzhou Medical University Hospital, No. 661 Huanghe 2nd Road, Binzhou, Shandong, 256603, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.169DOI Listing
July 2022

In Situ Fabrication of Magnetic and Hierarchically Porous Carbon Films for Efficient Electromagnetic Wave Shielding and Absorption.

ACS Appl Mater Interfaces 2022 Jul 14. Epub 2022 Jul 14.

School of Materials Science and Engineering, Xi'an Polytechnic University, Xi'an 710048, China.

Carbon-based materials have been recognized as a promising method to eliminate electromagnetic interference (EMI) shielding and electromagnetic (EM) wave absorption. However, developing lightweight, ultrathin, and efficient EM wave-shielding and wave-absorbing materials remains a challenge. Herein, a series of magnetic porous carbon composite films with a hierarchical network structure were fabricated via pyrolysis of porous polyimide (PI) films containing magnetic metallic salts of Fe(acac) and Ni(acac). After pyrolysis, the obtained uniform porous carbon films (CFs) possess a favorable EMI-shielding efficiency (SE) of 46 dB in the X-band with a thickness of ∼0.3 mm. In addition, a higher EMI SE of 58 dB can be achieved by increasing the thickness of the porous CF-20Ni to 0.53 mm. Moreover, the CF-20Ni composites also present effective EM wave-absorbing performance of RL = - 30.2 dB with a loading amount of 20 wt % at 13.0 GHz owing to the hierarchically conductive carbon skeleton, magnetic Ni nanoparticles, and dielectric interlaced carbon nanotube cluster within the micropores. These novel lightweight and ultrathin porous CFs are expected to be attractive candidates for efficient EM wave absorption and EMI shielding.
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http://dx.doi.org/10.1021/acsami.2c05286DOI Listing
July 2022

Market incentives, carbon quota allocation and carbon emission reduction: Evidence from China's carbon trading pilot policy.

J Environ Manage 2022 Jul 9;319:115650. Epub 2022 Jul 9.

School of International Trade and Economics, Anhui University of Finance and Economics, No. 962, Caoshan Road, 233030, Bengbu, China. Electronic address:

As a major carbon dioxide-emitting country, China set carbon trading market to reduce enterprise carbon emissions through the rational allocation of carbon quotas among different enterprises and regions. The market has also conducted a preliminary exploration for the country to achieve carbon dioxide emissions peak in 2030 and carbon neutrality in 2060 while actively addressing the challenges of global climate change. This study analysed the emission reduction effect of China's carbon trading pilot policy, especially the role of carbon quota and carbon trading price. The analysis used county-level panel data from 1997 to 2017, regarded the implementation of the carbon trading pilot policy as a quasi-natural experiment, and used the difference-in-differences method. The results showed that, first, the policy implementation not only reduced regional carbon emissions but also inhibited carbon dioxide emissions per capita, with long-term effects. Second, the carbon emission reduction effect brought by the carbon pilot policy showed significant heterogeneous results with the different degrees of regional carbon emissions and environmental supervision. The effect was greater in areas with higher carbon emission density and stronger legal supervision. Third, the difference in carbon quota allocations resulted in different emission reduction effects, among which the historical method had the strongest effect. The carbon quota price and number of enterprises participating carbon trading market were the key factors affecting carbon emission reduction.
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http://dx.doi.org/10.1016/j.jenvman.2022.115650DOI Listing
July 2022

Comparative genome anatomy reveals evolutionary insights into a unique amphitriploid fish.

Nat Ecol Evol 2022 Jul 11. Epub 2022 Jul 11.

State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, University of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Triploids are rare in nature because of difficulties in meiotic and gametogenic processes, especially in vertebrates. The Carassius complex of cyprinid teleosts contains sexual tetraploid crucian carp/goldfish (C. auratus) and unisexual hexaploid gibel carp/Prussian carp (C. gibelio) lineages, providing a valuable model for studying the evolution and maintenance mechanism of unisexual polyploids in vertebrates. Here we sequence the genomes of the two species and assemble their haplotypes, which contain two subgenomes (A and B), to the chromosome level. Sequencing coverage analysis reveals that C. gibelio is an amphitriploid (AAABBB) with two triploid sets of chromosomes; each set is derived from a different ancestor. Resequencing data from different strains of C. gibelio show that unisexual reproduction has been maintained for over 0.82 million years. Comparative genomics show intensive expansion and alterations of meiotic cell cycle-related genes and an oocyte-specific histone variant. Cytological assays indicate that C. gibelio produces unreduced oocytes by an alternative ameiotic pathway; however, sporadic homologous recombination and a high rate of gene conversion also exist in C. gibelio. These genomic changes might have facilitated purging deleterious mutations and maintaining genome stability in this unisexual amphitriploid fish. Overall, the current results provide novel insights into the evolutionary mechanisms of the reproductive success in unisexual polyploid vertebrates.
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http://dx.doi.org/10.1038/s41559-022-01813-zDOI Listing
July 2022

Response of microbiota and immune function to different hypotonic stress levels in giant freshwater prawn Macrobrachium rosenbergii post-larvae.

Sci Total Environ 2022 Jul 8:157258. Epub 2022 Jul 8.

Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.

This study explored the effects of different hypotonic stress levels on antioxidant capacity, microbial composition, and gene expression of Macrobrachium rosenbergii post-larvae. The salinity of the control group was 15 ‰ (S15), and the hypotonic stress groups included three levels of 10 ‰ (S10), 8 ‰ (S8), and 6 ‰ (S6). Different hypotonic stress levels caused oxidative damage in post-larvae, evidenced by decreased superoxide dismutase (SOD) and anti-superoxide anion free radical (ASAFR). They increased malondialdehyde (MDA), nitric oxide (NO), and inducible nitric oxide synthase (iNOS) levels. Microbiological analysis exhibited that different hypotonic stress levels significantly changed microbial composition and diversity. The microbial composition in the water environment where post-larvae living was different from post-larvae. The pathogenic bacteria, including Vibrio and Flavobacterium, were abundant in S6. Transcriptome analysis showed 2, 7967, 297 DEGs, including 1, 3564, 27 up-regulated genes and 1, 4403, 270 down-regulated genes in S10, S8, and S6 groups, respectively. KEGG enrichment results showed that immune and glucose metabolism-related pathways were enriched significantly. Correlation network analysis demonstrated close interactions among antioxidant parameters, microbes, and differentially-expressed genes. In conclusion, hypotonic stress reduced the antioxidant capacity, caused oxidative damage, and altered microbial composition in M. rosenbergii post-larvae. Moreover, when the salinity is below 8 ‰, hypotonic stress impairs the immune system of M. rosenbergii post-larvae.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157258DOI Listing
July 2022

An integrative pan-cancer analysis of the molecular and biological features of glycosyltransferases.

Clin Transl Med 2022 Jul;12(7):e872

Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of Carcinogenesis and Cancer Invasion (Ministry of Education), Fudan University, Shanghai, P. R. China.

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http://dx.doi.org/10.1002/ctm2.872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270580PMC
July 2022

Sensitive and rapid determination of heat shock protein 70 using lateral flow immunostrips and upconversion nanoparticle fluorescence probes.

Analyst 2022 Jul 22;147(15):3444-3450. Epub 2022 Jul 22.

Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, Tulane University School of Medicine, New Orleans, LA, USA.

Heat shock protein 70 (Hsp70), belonging to the heat shock protein (HSP) family, is reported to be a potential diagnostic biomarker. In this work, a lateral flow immunostrip was fabricated for the sensitive and rapid determination of Hsp70 by the incorporation of fluorescence and upconversion nanoparticle probes. The upconversion nanoparticles (UCNPs, size ∼39 nm, = 980 nm; = 540 nm) consisting of a NaYF4:Yb/Er core and polyacrylic acid-modified shell were covalently coupled with Hsp70 antibodies to form the signal probe, which was characterized by dynamic light scattering and zeta potential analyses. The lateral flow assay (LFA) was constructed based on the sandwich-type immunoassay using a sample pad, a test pad, and an adsorption pad on a PVP backing. Hsp70 antibody, IgG antibody and the signal probe were separately dropped on the test zone, the control zone of the test pad, and the sample pad, respectively. In the sandwich LFA, since two antibodies bind to Hsp70 antigenic epitopes, specific binding, it provided superior specificity and high sensitivity, making it an ideal sensing platform for complex samples like serum Hsp70 samples. The important parameters for the preparation of the lateral flow immunostrips were optimized. Under the optimized conditions, Hsp70 can be detected using the increased fluorescence intensity of UCNPs with a wide linear range from 0.11 to 12 ng mL, low detection limit of 0.06 ng mL, small sample volume (120 μL), short assay time (15 min) and good reproducibility. The fluorescence method was successfully applied in the determination of Hsp70 in serum samples with good recovery. By combining the accessibility of the lateral flow immunostrips and upconversion nanoparticles, the fluorescence method can serve as a point-of-care testing method for protein assays with high sensitivity and fast detection.
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http://dx.doi.org/10.1039/d2an00742hDOI Listing
July 2022

Varicella outbreaks in schools and kindergartens in Shanghai, China from 2011 to 2020.

PLoS One 2022 30;17(6):e0270630. Epub 2022 Jun 30.

Department of Immunization, Huangpu District Center for Disease Control and Prevention, Shanghai, China.

Background: Varicella is a contagious disease caused by varicella-zoster virus and varicella vaccine (VarV) is the most effective way to prevent and control varicella. Despite high VarV coverage there were still varicella outbreaks in schools and kindergartens. We aim to analyze the epidemiological characteristics of varicella outbreaks in Huangpu District, Shanghai, China from 2011 to 2020.

Methods: For varicella outbreaks, case information and vaccination history were collected. Mann-Kendall test and descriptive methods were used to analyzed the trend and epidemiological catachrestic of varicella outbreaks.

Results: A total of 57 varicella outbreaks were reported from 2011 to 2020, including 30 outbreaks (52.6%) in primary schools. The results of the Mann-Kendall trend test (z = 1.97, p = 0.049) showed an upward trend in the number of cases during the study period, but the trend change was not statistically significant. Emergency vaccination was carried out in 42 (73.7%) outbreaks which influenced the duration of the epidemic (F = 4.53, p = 0.0379). A total of 573 varicella cases were reported, including 357 cases (62.3%) who had received at least one dose of varicella vaccine.

Conclusions: The number of varicella outbreaks has not changed significantly in the decade from 2011 to 2020. The strategy of varicella vaccination, the development and application of varicella vaccine, and the control measures after the occurrence of varicella outbreaks need to be optimized. In addition to vaccination, as a disease transmitted by contact, quarantine measures, good personal hygiene, environmental disinfection, and ventilation are also important.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270630PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246396PMC
June 2022

A rhabdovirus accessory protein inhibits jasmonic acid signaling in plants to attract insect vectors.

Plant Physiol 2022 Jun 30. Epub 2022 Jun 30.

State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

Plant rhabdoviruses heavily rely on insect vectors for transmission between sessile plants. However, little is known about the underlying mechanisms of insect attraction and transmission of plant rhabdoviruses. In this study, we used an arthropod-borne cytorhabdovirus, Barley yellow striate mosaic virus (BYSMV), to demonstrate the molecular mechanisms of a rhabdovirus accessory protein in improving plant attractiveness to insect vectors. Here, we found that BYSMV-infected barley (Hordeum vulgare L.) plants attracted more insect vectors than mock-treated plants. Interestingly, overexpression of BYSMV P6, an accessory protein, in transgenic wheat (Triticum aestivum L.) plants substantially increased host attractiveness to insect vectors through inhibiting the jasmonic acid (JA) signaling pathway. The BYSMV P6 protein interacted with the constitutive photomorphogenesis 9 signalosome subunit 5 (CSN5) of barley plants in vivo and in vitro, and negatively affected CSN5-mediated deRUBylation of cullin1 (CUL1). Consequently, the defective CUL1-based SCF ubiquitin E3 ligases could not mediate degradation of jasmonate ZIM-domain proteins (JAZs), resulting in compromised JA signaling and increased insect attraction. Overexpression of BYSMV P6 also inhibited JA signaling in transgenic Arabidopsis (Arabidopsis thaliana) plants to attract insects. Our results provide insight into how a plant cytorhabdovirus subverts plant JA signaling to attract insect vectors.
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http://dx.doi.org/10.1093/plphys/kiac319DOI Listing
June 2022

Salinity and seasonality shaping free-living and particle-associated bacterioplankton community assembly in lakeshores of the northeastern Qinghai-Tibet Plateau.

Environ Res 2022 Jun 24;214(Pt 1):113717. Epub 2022 Jun 24.

Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai, China; Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming, 650091, China.

Microorganisms in lakeshore zones are essential for pollution interception and biodiversity maintenance. However, the biogeographic patterns of bacterioplankton communities in lakeshore zones and the mechanisms that driving them are poorly understood. We analyzed the 16 S rRNA gene sequences of particle-associated (PA) and free-living (FL) bacterioplankton communities in the lakeshore zones of 14 alpine lakes in two seasons on Qinghai-Tibet Plateau to investigate the bacterial diversity, composition and assembly processes. Our results revealed that PA and FL bacterioplankton communities were driven by both seasonality and salinity in the lakeshores on Qinghai-Tibet Plateau. Compared to FL bacterioplankton, PA bacterioplankton communities were more susceptible to seasonality than spatial salinity. FL bacterioplankton communities were more salinity constrained than the PA counterpart. Besides, the Stegen null model analyses have validated a quantitative bias on stochastic processes at different spatial scales. At a regional scale, stochasticity was the predominant assembly process in both PA and FL bacterioplankton. While at a subregional scale, dispersal limitation was the main contributor of stochastic processes for PA bacterioplankton in summer and heterogeneous selection was the dominant deterministic processes in winter, whereas the community assembly of FL bacterioplankton was more stochastic processes (i.e., dispersal limitation) dominated in the freshwater type but deterministic process (i.e., heterogeneous selection) increased with increasing salinity. Our study provides new insights into both significant spatiotemporal patterns and distinct assembly processes of PA and FL bacterioplankton in alpine lakeshores on the northeastern Qinghai-Tibet Plateau.
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http://dx.doi.org/10.1016/j.envres.2022.113717DOI Listing
June 2022

Investigation on the Temperature Control Accuracy of a Print Head for Extrusion 3D Printing and Its Improved Design.

Biomedicines 2022 May 25;10(6). Epub 2022 May 25.

School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.

For the extrusion 3D printing process, the printing temperature has a significant impact on the filament formation process because the rheological properties of the printed materials are extremely thermal sensitive, which requires a high temperature control accuracy of the print head. This paper presents a numerical and experimental investigation on the temperature field of a homemade print head. A finite element simulation model for analyzing the temperature field of the print head was established, by which the temperature distribution inside the print head can be acquired. Moreover, to improve the temperature control accuracy, an improved configuration was proposed, and two schemes were compared. The temperature control error dropped from 28% to 6.2% with the improved print head, which was verified experimentally. Furthermore, printing trials were conducted by the optimized print head. The filament diameter could be regulated by changing the temperature of the print head, which validates the feasibility to control the filament diameter during the extrusion process via temperature regulation.
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http://dx.doi.org/10.3390/biomedicines10061233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220126PMC
May 2022

Fine mapping of the tiller inhibition gene TIN5 in Triticum urartu.

Theor Appl Genet 2022 Aug 22;135(8):2665-2673. Epub 2022 Jun 22.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

Key Message: A tiller inhibition gene TIN5 was delimited to an approximate 2.1 Mb region on chromosome Tu7 that contains 24 annotated genes. Grain yield in wheat (Triticum aestivum L.) is a polygenic trait representing many developmental processes and their interactions with the environments. Among them, tillering capacity is an important agronomic trait for plant architecture and grain yield, but the genetic basis of tiller formation in wheat remains largely unknown. In this study, we identified a tiller inhibition 5 (tin5) mutant from ethyl methane sulfonate treated G1812 (Triticum urartu Thumanjan ex Gandilyan). A mapping population was constructed with tin5/G3146. Based on the sequence differences between G1812 and G3146, large insertions and deletions (≥ 5 bp) were selected and verified, and a skeleton physical map was constructed with genome-wide 168 polymorphic InDel markers. Genetic analysis revealed that the low-tiller phenotype was controlled by a single recessive locus, which we named TIN5. This locus was mapped to a 2.1-Mb region that contained 24 annotated genes on chromosome Tu7. Among these annotated genes, only TuG1812G0700004539 showed a non-synonymous polymorphism between tin5 and the wild type. Our finding will facilitate its map-based cloning and pave the way for an in-depth analysis of the underlying genetic basis of tiller formation and regulation patterns.
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http://dx.doi.org/10.1007/s00122-022-04140-wDOI Listing
August 2022

Biomimetic lignin-protein adhesive with dynamic covalent/hydrogen hybrid networks enables high bonding performance and wood-based panel recycling.

Int J Biol Macromol 2022 Aug 10;214:230-240. Epub 2022 Jun 10.

MOE Key Laboratory of Wooden Material Science and Application & Beijing Key, Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address:

Exploring the reusability of wood-based panels is imperative in the wood industry for sustainable development and carbon balance. Non-reusable adhesives make wood-based panel recycling difficult. In this study, inspired by the adhesion and de-adhesion behavior of snail slime, we built dynamic covalent/hydrogen hybrid networks into adhesive system for achieving both high bonding performance and reusability. Specifically, the softwood lignin was purified and pretreated by ultrasonication to form a catechol structure (UAL) and then combined with soybean protein to develop a 100 % bio-based wood adhesive. The catechol structure of UAL formed dynamic covalent bonds (CN) with the amino groups of the protein to improve the water resistance and formed multiple hydrogen bonds as a sacrificial network to improve the toughness of the adhesive. Thus, the wet shear strength of plywood bonded by the resultant adhesive improved by 101.4 % to 1.37 MPa. The adhesive also exhibited flame retardancy (LOI = 37.7 %), mildew resistance (60 h), and antibacterial performance (inhibition zone = 8 mm). Notably, owing to the rearrangement of dynamic covalent/hydrogen hybrid networks and the thermoplastic property of UAL, the resultant adhesive was reusable (3 cycles) and degradable (2 months), which provides a potential method for the reuse of wood-based panels.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.042DOI Listing
August 2022
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