Publications by authors named "Qiang Chen"

1,866 Publications

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Over-expression of IL1R2 in PBMCs of Patients with Coronary Artery Disease and Its Clinical Significance.

Anatol J Cardiol 2022 Aug 9. Epub 2022 Aug 9.

Department of Cardiology, Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, China.

Background: IL-1 has been widely explored and played a role in regulating inflammatory and immune responses to various disorders. Nevertheless, the role of interleukin-1 recep- tor type II, a protein-coding gene of interleukin-1 in coronary artery disease patients with peripheral blood mononuclear cells, persists to be undetermined.

Methods: Our study discovered the IL-1 receptor type II expression through gene expres- sion omnibus (GEO) public repository based on bioinformatics tools and further valida- tion was carried out between coronary artery disease patients and healthy participants using peripheral blood mononuclear cells samples in Second Hospital of Tianjin Medical University. A total of 180 participants, comprising 90 cases of coronary artery disease and 90 samples of healthy control were retrospectively evaluated and the correlation of IL-1 receptor type II was observed between serum levels of oxidized low-density lipopro- tein and SYNTAX score. Furthermore, the clinical significance of IL-1 receptor type II was evaluated in peripheral blood mononuclear cells of coronary artery disease patients by the receiver operating curve using the area under the curve.

Results: IL-1 receptor type II was markedly overexpressed in peripheral blood mono- nuclear cells and severe patients with coronary artery disease compared to the healthy control participants. Meanwhile, a positive correlation of IL-1 receptor type II expression was significantly observed between SYNTAX score and oxidized low-density lipoprotein of coronary artery disease patients. Further, the receiver operating curve achieved a sig- nificantly higher area under the curve of 0.813 in coronary artery disease patients with peripheral blood mononuclear cells. Thus, IL-1 receptor type II expressions were not only directly correlated with peripheral blood mononuclear cells but also showed potential significance in coronary artery disease patients.

Conclusion: IL-1 receptor type II might be involved in the immune/inflammatory responses of coronary artery disease accompanied by other cytokine receptor genes.
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http://dx.doi.org/10.5152/AnatolJCardiol.2022.1241DOI Listing
August 2022

Stroke risk evaluation for patients with atrial fibrillation: Insights from left atrial appendage with fluid-structure interaction analysis.

Comput Biol Med 2022 Jul 30;148:105897. Epub 2022 Jul 30.

School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Brisbane, QLD4001, Australia. Electronic address:

The majority of cardioembolic strokes in patients with non-valvular atrial fibrillation (NVAF) are resulted from clot formation in the left atrial appendage (LAA). Current stroke risk stratification is based on the overall risks estimated from demographic and clinical profiles but not on individual anatomy or physiology. We aim to explore the differences in LAA morphological and hemodynamic parameters by comparing patients with and without a stroke history. Thirty-nine patients with persistent NVAF were included. Of these, 17 patients without a stroke history (non-stroke group) were compared with 22 patients with a history of stroke (stroke group). Their LAA geometric models were first reconstructed, and the morphological parameters were then measured. Furthermore, their LAA hemodynamic parameters were calculated by fluid-structure interaction analysis. Moreover, particle residual rates (PRR) and blood renewal rates (BRR) analyses were also employed to characterize the thrombogenesis dynamics. The results showed that compared to the non-stroke group, the stroke group had significant smaller LAA tortuosity and LAA orifice area, and significantly lower LAA orifice velocities (0.16 ± 0.10 vs 0.15 ± 0.06 cm/s; p = 0.044), but higher PRR (14.58 ± 9.43 vs 9.25 ± 4.67; p = 0.040) and BRR (52.41 ± 18.11 vs 38.36 ± 24.07; p = 0.044). These LAA morphological and hemodynamic parameters may be used to assess stroke risk in patients with NVAF.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105897DOI Listing
July 2022

Promoting public engagement with household waste separation through government social media: A case study of Shanghai.

J Environ Manage 2022 Aug 3;320:115825. Epub 2022 Aug 3.

Faculty of Computer Science, Dalhousie University, Halifax, Canada.

Government agencies around the world are increasingly using social media to promote public engagement with government-initiated activities, such as Household Waste Separation (HWS). However, existing studies fail to examine how governments can use their official social media accounts to promote public engagement with HWS. This pioneering study investigates the effects of dialogic loop, media richness, content theme, and emotional valence on public engagement with HWS through Government Social Media (GSM). Moreover, the influencing mechanisms are explored by examining the moderating role of social media capital. In total, 1795 posts were scraped from the official Sina Weibo accounts of seventeen Shanghai governments and later analyzed. Results reveal that dialogic loop positively predicts public engagement with HWS through GSM, but media richness negatively affects public engagement. Content themes have significantly different impacts on the level of public engagement with HWS through GSM. Unexpectedly, the effect of emotional valence is not significant. This study is the first of its kind to demonstrate the moderating role of social media capital which is seen to weaken the positive effect of dialogic loop on public engagement with HWS through GSM and mitigate the negative effect of media richness.
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http://dx.doi.org/10.1016/j.jenvman.2022.115825DOI Listing
August 2022

Adjustment of the GRACE score by the triglyceride glucose index improves the prediction of clinical outcomes in patients with acute coronary syndrome undergoing percutaneous coronary intervention.

Cardiovasc Diabetol 2022 Aug 5;21(1):145. Epub 2022 Aug 5.

Department of Cardiology, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610014, Sichuan, China.

Background: The Global Registry of Acute Coronary Events (GRACE) score derived from clinical parameters at the time of hospital discharge is a powerful predictor of long-term mortality and reinfarction after acute coronary syndrome (ACS). The triglyceride glucose (TyG) index, which is a simple and reliable surrogate marker of insulin resistance, has been demonstrated to be an independent predictor of long-term adverse major adverse cardiac events, irrespective of diabetes mellitus. We investigate whether the addition of the TyG index improves the predictive ability of the GRACE score after percutaneous coronary intervention (PCI) in ACS patients regardless of diabetes mellitus.

Method: A retrospective cohort of 986 ACS patients undergoing PCI was enrolled in the present analyses. The GRACE score for discharge to 6 months and the TyG index were calculated. The primary endpoint was the composite of MACEs, including all-cause death and nonfatal myocardial infarction. Patients were stratified according to the primary endpoint and the tertiles of the TyG index. Cumulative curves were calculated using the Kaplan-Meier method. Multivariate Cox regression was adopted to identify predictors of MACEs. The predictive value of the GRACE score alone and combined with the TyG index or fasting blood glucose (FBG) was estimated by the area under the receiver‑operating characteristic curve, likelihood ratio test, Akaike's information criteria, continuous net reclassification improvement (NRI), and integrated discrimination improvement (IDI). Internal validation was assessed using the means of bootstrap method with 1000 bootstrapped samples.

Results: During a median follow-up of 30.72 months ((interquartile range, 26.13 to 35.07 months), 90 patients developed MACEs, more frequently in the patients with a higher TyG index. Multivariate Cox hazards regression analysis found that the TyG index, but not FBG was an independent predictor of MACEs (hazard ratio 1.6542; 95% CI 1.1555-2.3681; P = 0.006) in all types of ACS regardless of diabetes mellitus when included in the same model as GRACE score. Furthermore, Kaplan-Meier analysis revealed that the incidence of the primary endpoint rose with increasing TyG index tertiles (log-rank, P < 0.01). Adjustment the GRACE score by the TyG index improved the predictive ability for MACEs (increase in C-statistic value from 0.735 to 0.744; NRI, 0.282, 95% CI 0.028-0.426, P = 0.02; IDI, 0.019, 95% CI 0.004-0.046, P = 0.01). Likelihood ratio test showed that the TyG index significantly improved the prognostic ability of the GRACE score (χ = 12.37, 1 df; P < 0.001). The results remained consistent when the models were confirmed by internal bootstrap validation method.

Conclusion: The TyG index, but not FBG is an independent predictor of long-term MACEs after PCI in all types of ACS patients regardless of diabetes mellitus after adjusting for the GRACE score, and improves the ability of the GRACE score to stratify risk and predict prognosis of ACS patients undergoing PCI.
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http://dx.doi.org/10.1186/s12933-022-01582-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356419PMC
August 2022

Pretracheal Lymph Node Subdivision in Predicting Contralateral Central Lymph Node Metastasis for Unilateral Papillary Thyroid Carcinoma: Preliminary Results.

Front Endocrinol (Lausanne) 2022 18;13:921845. Epub 2022 Jul 18.

Department of Thyroid and Parathyroid Surgery Center, West China Hospital, Sichuan University, Chengdu, China.

Background: The aims of this study were to assess the clinical value of pretracheal lymph node subdivision in identifying patients with contralateral central lymph node metastasis (CLNM) and risk factors for occult contralateral CLNM in unilateral PTC.

Methods: A total of 139 unilateral PTC patients with a clinically node-negative neck (cN0) who underwent bilateral central neck dissection (CND) were prospectively enrolled. Intraoperatively, the pretracheal region was further divided into ipsilateral and contralateral subregions. Ipsilateral and contralateral pretracheal lymph nodes (LNs) as well as other CLNs (prelaryngeal, ipsilateral paratracheal and contralateral paratracheal) were labeled separately and sent for pathological examination. Demographic and clinicopathologic variables were analyzed to identify factors predictive of contralateral CLNM.

Results: Of 139 patients, bilateral CLNM was present in 37 (26.6%) patients. Contralateral pretracheal LNM was significantly associated with contralateral CLNM. In multivariate analysis, prelaryngeal LNM ( = 0.004, odds ratio = 3.457) and contralateral pretracheal LNM ( = 0.006, odds ratio = 3.362) were identified as risk factors for contralateral CLNM. Neither neck recurrence nor distant metastasis was observed within the mean follow-up duration of 9.1 ± 1.8 months.

Conclusions: In most unilateral cN0 PTCs, performing ipsilateral CND is appropriate, while patients presenting with evident nodal disease intraoperatively or preoperatively in the contralateral central neck should undergo bilateral CND. Intraoperative re-evaluation of prelaryngeal and contralateral pretracheal LNs may be helpful in determining the extent of CND.
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http://dx.doi.org/10.3389/fendo.2022.921845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339796PMC
August 2022

Effects of pulmonary fissure completeness on major outcomes in children after video-assisted thoracoscopic congenital lung malformation lobectomy.

BMC Pediatr 2022 08 1;22(1):462. Epub 2022 Aug 1.

Department of Cardiac Surgery, Fujian Branch of Shanghai Children's Medical Center, 966 Hengyu Road, Fuzhou City, Fujian Province, China.

We performed a single-centre retrospective analysis using data from databases that were prospectively maintained in our centre between January 2019 and September 2021. Patients were divided into two groups based on the degree of pulmonary fissure completeness (PFC), using the fissure development scoring system. Patients with grades 2 or 3 PFC were considered to have incomplete pulmonary fissures and were included in Group A, and patients with grades 0 and 1 were considered to have complete pulmonary fissures and were included in Group B. The differences in demographics, perioperative characteristics and clinic outcomes between the two groups were evaluated. Multivariate logistic regression analysis was performed. A total of 213 patients with congenital lung malformation (CLM) underwent video-assisted thoracoscopic lobectomy. There were 30 patients in Group A and 183 patients in Group B. Our data showed that compared with Group B, Group A had a higher incidence of complications, especially Clavien-Dindo grade II and grade III complications. The degree of PFC was significantly correlated with the length of chest tube drainage and postoperative hospital stay. Multivariate logistic regression analysis showed that the degree of PFC could be used to predict the incidence of postoperative complications.ConclusionsThe degree of PFC is a predictor of the incidence of complications after thoracoscopic lobectomy in children with CLM.
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http://dx.doi.org/10.1186/s12887-022-03527-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341063PMC
August 2022

Rescue high-frequency oscillatory ventilation combined with intermittent mandatory ventilation for infants with acute respiratory distress syndrome after congenital heart surgery.

Cardiol Young 2022 Aug 1:1-7. Epub 2022 Aug 1.

Department of Cardiac Surgery, Fujian Branch of Shanghai Children's Medical Center, Fuzhou, China.

Background: This study aimed to evaluate the efficacy and safety of high-frequency oscillation ventilation combined with intermittent mandatory ventilation in infants with acute respiratory distress syndrome after congenital heart surgery.

Methods: We retrospectively analysed the clinical data of 32 infants who were ventilated due to acute respiratory distress syndrome after congenital heart surgery between January, 2020 and January, 2022. We adopted high-frequency oscillation ventilation combined with intermittent mandatory ventilation as the rescue ventilation mode for infants who were failing conventional mechanical ventilation.

Results: After rescue high-frequency oscillation ventilation combined with intermittent mandatory ventilation, the dynamic compliance (Cdyn), PaO and PaO/FiO ratio of the infants improved compared with conventional mechanical ventilation (p < 0.05). Moreover, high-frequency oscillation ventilation combined with intermittent mandatory ventilation resulted in a significant decrease in arterial-alveolar oxygen difference (AaDO), FiO, and oxygenation index (p < 0.05). No significant effect on haemodynamic parameters was observed. Moreover, no serious complications occurred in the two groups.

Conclusion: Rescue high-frequency oscillation ventilation combined with intermittent mandatory ventilation significantly improved oxygenation in infants who failed conventional mechanical ventilation for acute respiratory distress syndrome after congenital heart surgery. Thus, this strategy is considered safe and feasible. However, further studies must be conducted to confirm the efficacy and safety of high-frequency oscillation ventilation combined with intermittent mandatory ventilation as a rescue perioperative respiratory support strategy for CHD.
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http://dx.doi.org/10.1017/S1047951122002396DOI Listing
August 2022

Silencing Promotes the Proliferation of Spermatogonia in the Testis of Experimental Varicocele Rats.

Evid Based Complement Alternat Med 2022 19;2022:7121245. Epub 2022 Jul 19.

Department of Andrology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.

Varicocele is regarded as the main factor that contributes to male infertility. This study aimed to explore the effect of on spermatogonia in the testis of experimental varicocele rats. The experimental varicocele model was established in rats and treated using different ligation methods. mRNA expression profile analysis was performed on the left testicular tissue isolated from different groups, and differentially expressed genes (DEGs) were analysed by bioinformatics methods and identified by qRT-PCR. The effect of , the screened DEG, on the proliferation of spermatogonia was evaluated by CCK-8 assay. The expression level of the c-kit was measured by the immunofluorescence assay and the expression levels of , , and were detected by qRT-PCR and western blotting. Five DEGs (i.e., , , , , and ) were screened using the comprehensive analysis of mRNA high-throughput sequencing data. and were significantly downregulated, and , , and were dramatically upregulated in the testicular tissue of varicocele rats. The target DEG was obtained through identification by using qRT-PCR. assays revealed that the proliferation of spermatogonia was significantly facilitated by the silencing of , which resulted in the downregulation of , , and . In conclusion, silencing facilitated the proliferation of spermatogonia in the testis of experimental varicocele rats.
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http://dx.doi.org/10.1155/2022/7121245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325628PMC
July 2022

A bottom-up approach from medium-sized bilayer boron nanoclusters to bilayer borophene nanomaterials.

Nanoscale 2022 Aug 11;14(31):11443-11451. Epub 2022 Aug 11.

Nanocluster Laboratory, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.

Inspired by the experimentally observed bilayer B and theoretically predicted bilayer B-B and based on extensive density functional theory calculations, we report herein a series of novel medium-sized bilayer boron nanoclusters B (I), B (II), B (III), B (IV), B (V), B (VI), B (VII), and B (VIII) which are the most stable isomers of the systems reported to date effectively stabilized by optimum numbers of interlayer B-B σ bonds between the inward-buckled atoms on top and bottom layers. Detailed bonding analyses indicate that these bilayer species follow the universal bonding pattern of σ + π double delocalization, rendering three-dimensional aromaticity in the systems. More interestingly, the AA-stacked bilayer structural motif in B (VII) with a B bilayer hexagonal prism at the center can be extended to form bilayer B (IX), B (X), B (XI), B (XII), and B (XIII) which contain one or multiple conjoined B bilayer hexagonal prisms sharing interwoven zig-zag boron triple chains between them. Such bilayer species or their close-lying AB isomers can be viewed as embryos of the newly reported most stable freestanding BL-α bilayer borophenes and quasi-freestanding bilayer borophenes on Ag(111) which are composed of interwoven zig-zag boron triple chains shared by conjoined BL B hexagonal prisms, presenting a bottom-up approach from medium-sized bilayer boron nanoclusters to two-dimensional bilayer borophene nanomaterials.
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http://dx.doi.org/10.1039/d2nr02950bDOI Listing
August 2022

Case Report: A Case of Late-Onset Combined Methylmalonic Acidemia and Hyperhomocysteinemia Induced by a Vegetarian Diet.

Front Pediatr 2022 12;10:896177. Epub 2022 Jul 12.

Department of Pediatrics, Baoding No. 1 Central Hospital, Baoding, China.

Methylmalonic acidemia is a rare autosomal recessive metabolic disease. However, because of the atypical clinical symptoms, the type of late-onset methylmalonic academia is often misdiagnosed. Especially when the blood vitamin B12 and folic acid levels are normal, it is not easy to think of this disease. Herein we report a 9-year-old girl who developed normally on a relatively balanced diet before 7 years of age. However, she presented with fatigue and attention deficit when she followed a vegetarian diet. Laboratory examination showed moderate macrocytic anemia, high levels of homocysteine, high level of propionylcarnitine/acetylcarnitine, urinary methylmalonic acid and methyl citrate. Gene mutation analysis showed c.609G > A and c.80A > G compound heterozygous mutations in the gene, supported late-onset combined methylmalonic academia with homocysteinemia. Then treatment performed with add meat to the diet, vitamin B12, folic acid betaine and L-carnitine supplement. One week later, the child's clinical symptoms and the laboratory examinations were significantly improved.
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http://dx.doi.org/10.3389/fped.2022.896177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315243PMC
July 2022

Tough Wet Adhesion of Hydrogen-Bond-Based Hydrogel with On-Demand Debonding and Efficient Hemostasis.

ACS Appl Mater Interfaces 2022 Aug 28;14(31):36166-36177. Epub 2022 Jul 28.

Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, Zhejiang, P. R. China.

Hydrogels have been widely used in wet tissues. However, the insufficient adhesion of hydrogels for wound hemostasis remains a grand challenge. Herein, a facile yet effective strategy is developed to fabricate tough wet adhesion of hydrogen-bond-based hydrogel (PAAcVI hydrogel) using copolymerization of acrylic acid and 1-vinylimidazole in dimethyl sulfoxide followed by solvent exchange with water. The PAAcVI hydrogel shows equally robust adhesion (>400 J m) to both wet and dry tissues. Moreover, the PAAcVI hydrogel also exhibits strong long-term stable adhesion underwater and in various wet environments. Meanwhile, the adhesion of PAAcVI hydrogel can be adjusted through Zn-ion-mediated on-demand debonding, which makes it easy to peel off from the tissue reducing pain during dressing removal and avoiding secondary injury. The PAAcVI hydrogel displays efficient hemostasis in the mice-tail docking model and mice-liver bleeding model. This hydrogen-bond-based hydrogel shows tough wet adhesion, and its adhesion is controllable, demonstrating its promising application in moisture-resistant adhesives, medical adhesives, and hemostatic materials.
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http://dx.doi.org/10.1021/acsami.2c10202DOI Listing
August 2022

Immune Cytolytic Activity for Comprehensive Insights of the Immune Landscape in Endometrial Carcinoma.

J Oncol 2022 18;2022:9060243. Epub 2022 Jul 18.

Department of Clinical Pathology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, China.

Immune checkpoint blockade (ICB) has been explored as a therapeutic strategy to recover the antitumor immune activities against endometrial cancer (EC) escaping from immune surveillance. Increasing evidence has indicated that microsatellite instability (MSI) is a promising biomarker to stratify patients for the ICB therapy. However, even in patients with MSI-High (MSI-H) endometrial cancers, PD-L1 inhibitors, avelumab, and durvalumab have shown only 27% of response rates. Therefore, there is an urgent need to discover new biomarkers for a predictive response to ICB therapy. In this study, we demonstrated that the immune cytolytic activity (CYT) index was significantly correlated with the development and response to immunotherapy in EC. The data showed that higher CYT was significantly associated with better clinical outcome, more antitumor infiltrating immune cells, fewer somatic copy number alterations, but a higher TMB (Tumor mutational burden) status. Furthermore, CYT-high EC was notably relevant to the high expression of various immune checkpoint molecules and showed more effective responses to ICB treatment. Taken together, this study provided new insights into the connection between diverse genetic events and the immune microenvironment in EC and indicated that the CYT status might be a promising biomarker to stratify patients with EC for ICB therapy.
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http://dx.doi.org/10.1155/2022/9060243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313908PMC
July 2022

LINC00265 Promotes Metastasis and Progression of Hepatocellular Carcinoma by Interacting with E2F1 at The Promoter of .

Cell J 2022 Jun 29;24(6):294-301. Epub 2022 Jun 29.

Department of Psychiatry, Guangxi Zhuang Autonomous Region Brain Hospital, Liuzhou, Guangxi, China. Email:

Objective: This study aimed to explore biological function of long intergenic non-protein coding RNA 265 (LINC00265) in hepatocellular carcinoma (HCC) cells, and evaluate its potential function as a biomarker.

Materials And Methods: In this experimental study, GEPIA database and Kaplan-Meier Plotter database were employed to analyze LINC00265 expression in HCC tissue samples and its predicting value for prognosis. LINC00265 expression in HCC tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). After overexpressing and knocking-down of LINC00265 in HCC cells, cell counting kit-8 (CCK-8) and 5-Ethynyl-2'- deoxyuridine (EdU) assays were adopted to detect proliferation of HCC cells. Transwell assay was used to detect migration and invasion of HCC cells. Interaction of LINC00265 with E2F transcription factor 1 (E2F1) was verified by the catRAPID online analysis tool, RNA pull-down experiment and RNA binding protein immunoprecipitation (RIP) assay. Binding of E2F1 to the promoter region of cyclin-dependent kinases 2 () was detected by dual-luciferase reporter assay and chromatin immunoprecipitation. Regulatory effects of LINC00265 and E2F1 on expression were probed by Western blot.

Results: LINC00265 expression was increased in HCC tissues and cells. LINC00265 overexpression promoted proliferation, migration and invasion of HCC cells, and knocking-down LINC00265 worked oppositely. LINC00265 could bind to E2F1 and it could enhance combination of E2F1 and promoter regions, thus promoting CDK2 transcription. LINC00265 overexpression promoted expression of in HCC cells.

Conclusion: Our data suggested that LINC00265 can promote malignant behaviors of HCC cells by recruiting E2F1 to the promoter region of .
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http://dx.doi.org/10.22074/cellj.2022.8035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315211PMC
June 2022

The Variance of Photophysical Properties of Tetraphenylethene and Its Derivatives during Their Transitions from Dissolved States to Solid States.

Polymers (Basel) 2022 Jul 15;14(14). Epub 2022 Jul 15.

Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.

The study of aggregation-induced emission luminogens (AIEgens) shows promising perspectives explored in lighting, optical sensors, and biological therapies. Due to their unique feature of intense emissions in aggregated solid states, it smoothly circumvents the weaknesses in fluorescent dyes, which include aggregation-caused quenching of emission and poor photobleaching character. However, our present knowledge of the AIE phenomena still cannot comprehensively explain the mechanism behind the substantially enhanced emission in their aggregated solid states. Herein, to systematically study the mechanism, the typical AIEgens tetraphenylethene (TPE) was chosen, to elucidate its photophysical properties, the TPE in THF/HO binary solvents, TPE in THF solvents depending on concentration, and the following direct conversion from a dissolved state to a precipitated solid state were analyzed. Moreover, the TPE derivatives were also investigated to supply more evidence to better decipher the generally optical behaviors of TPE and its derivatives. For instance, the TPE derivative was homogeneously dispersed into tetraethyl orthosilicate to monitor the variance of photophysical properties during sol-gel processing. Consequently, TPE and its derivatives are hypothesized to abide by the anti-Kasha rule in dissolved states. In addition, the factors primarily influencing the nonlinear emission shifting of TPE and its derivatives are also discussed.
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http://dx.doi.org/10.3390/polym14142880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320569PMC
July 2022

Initiator enhancement of mandrel degradation for ICF target fabrication.

iScience 2022 Aug 9;25(8):104733. Epub 2022 Jul 9.

Institute of Atomic and Molecular Physics, Jilin University, Changchun 130012, China.

Poly-α-methylstyrene (PAMS), as an ideal mandrel material used in the fabrication of inertial confinement fusion (ICF) targets, its efficient degradation is the key to the quality of targets. However, there is a great challenge to achieve enhanced degradation. Here, we proposed the strategy to optimize the degradation of PAMS microspheres using di--butyl peroxide (DTBP) as a degradation initiator. Experimentally, monodisperse PAMS microspheres with DTBP were controllably prepared by a microfluidic-based microencapsulation technique. Thermogravimetric results show that DTBP largely decreases the initial degradation temperature from 550 K to 450 K, which effectively promotes the thermal degradation of PAMS microspheres. Theoretically, DTBP can reduce the activation energy of degradation. Moreover, the potential energy surfaces were used to describe the degradation process at the atomic level. Our work brings a new direction for the study of mandrel degradation in ICF targets fabrication, and also provides a valuable reference for solving the pollution of waste plastics.
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http://dx.doi.org/10.1016/j.isci.2022.104733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307930PMC
August 2022

COVID-19 Pandemic: Insights into Interactions between SARS-CoV-2 Infection and MAFLD.

Int J Biol Sci 2022 11;18(12):4756-4767. Epub 2022 Jul 11.

Cancer Center, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China.

COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become an ongoing global health pandemic. Since 2019, the pandemic continues to cast a long shadow on all aspects of our lives, bringing huge health and economic burdens to all societies. With our in-depth understanding of COVID-19, from the initial respiratory tract to the later gastrointestinal tract and cardiovascular systems, the multiorgan involvement of this infectious disease has been discovered. Metabolic dysfunction-associated fatty liver disease (MAFLD), formerly named nonalcoholic fatty liver disease (NAFLD), is a major health issue closely related to metabolic dysfunctions, affecting a quarter of the world's adult population. The association of COVID-19 with MAFLD has received increasing attention, as MAFLD is a potential risk factor for SARS-CoV-2 infection and severe COVID-19 symptoms. In this review, we provide an update on the interactions between COVID-19 and MAFLD and its underlying mechanisms.
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http://dx.doi.org/10.7150/ijbs.72461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305262PMC
July 2022

Physiological and Biochemical Adaptations to High Altitude in Tibetan Frogs, .

Front Physiol 2022 6;13:942037. Epub 2022 Jul 6.

School of Life Sciences, Lanzhou University, Lanzhou, China.

The Xizang plateau frog, (Anura: Dicroglossidae), is endemic to the Tibetan Plateau, ranging from 2,850 to 5,100 m above sea level. The present study explores physiological and biochemical adaptations to high altitude in this species with a particular emphasis on parameters of hematology, oxidative stress, and antioxidant defense in adult and juvenile collected from high (4,600 m a.s.l) and low (3,400 m a.s.l) altitudes. Hematological results showed that hemoglobin concentration ([Hb]), hematocrit (Hct), and red blood cell (RBC) counts were significantly higher in high-altitude . High-altitude juveniles had lower RBC sizes than low-altitude juveniles. Higher levels of GSH and GSSG were indicated only in juveniles from high altitude, not in adults. High-altitude individuals also showed lower oxidative damage, assessed as malondialdehyde (MDA) and carbonyl groups (CG) in the liver. High-altitude adults also showed higher activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione-S-transferase (GST) as well as total antioxidant capacity (T-AOC) in the liver as compared to low-altitude adults. Moreover, higher GPX activity and T-AOC were observed in the heart and brain of high-altitude adults. Liver CAT, GPX, and T-AOC showed significant increases in high-altitude juveniles. Vitamin C content was also higher in the heart of high-altitude frogs compared to low-altitude individuals. In summary, the high-altitude population of showed more robust hematological parameters, less oxidative damage, and stronger antioxidant defenses than the low-altitude population, all contributing to increased protection for survival in high-altitude environments.
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http://dx.doi.org/10.3389/fphys.2022.942037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298763PMC
July 2022

Epidemiology and genetic characterization of human metapneumovirus in pediatric patients from Hangzhou China.

J Med Virol 2022 Jul 24. Epub 2022 Jul 24.

Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, Zhejiang, China.

Human metapneumovirus (HMPV), which is distributed worldwide, is a significant viral respiratory pathogen responsible for causing acute respiratory tract infections (ARTIs) in children. The aim of the present study was to investigate the epidemiological and genetic characteristics of HMPV in pediatric patients in Hangzhou China following the peak of onset of coronavirus disease 2019 (COVID-19). A total of 1442 throat swabs were collected from the pediatric patients with a diagnosis of ARTI from November 2020 to March 2021. The following viruses were detected by real-time polymerase chain reaction analysis: HMPV, RSV, adenovirus, hPIV1-3, influenza A, and influenza B. A two-step method was used to amplify the F genes of the HMPV-positive samples. Following sequencing, phylogenetic analyses were conducted using the MEGA version 7 software package. Among the 1442 samples, 103 (7.14%) were positive for HMPV. No significant differences were observed in the gender distribution. The highest incidence of HMPV occurred in children older than 6 years and the lowest was noted in children younger than 6 months. Lower respiratory tract infections were diagnosed at a higher rate than upper respiratory tract infections in HMPV-infected children. Only 10 HMPV-infected children (5.41%) were inpatients compared with 93 outpatients (7.39%). Co-infection was observed in 31 HMPV-positive samples including 24 samples of double infection and seven samples of triple infection. A total of 61F gene fragments of HMPV, which were approximately 727 bp in length were successfully sequenced. All the HMPVs belonged to the genotype B and were clustered into subgenotypes B1 (1.6%, 1/61) and B2 (98.4%, 60/61). A total of four specific amino acid substitutions were noted as follows: aa280, aa296, aa392, and aa396. These substitutions were present between sequences derived from the subgenotypes B1 and B2 in the fusion open reading frame from position 244 to 429. In conclusion, the present study provided significant information regarding the epidemiological and genetic characteristics of HMPV in children living in Hangzhou. Following the first peak of the COVID-19 pandemic, HMPV was considered an important viral respiratory pathogen present in children with ARTI.
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http://dx.doi.org/10.1002/jmv.28024DOI Listing
July 2022

Boosting the peroxidase-like activity of Pt nanozymes by a synergistic effect of TiC nanosheets for dual mechanism detection.

Dalton Trans 2022 Aug 9;51(31):11693-11702. Epub 2022 Aug 9.

Key Laboratory for Micro-Nano Materials for Energy Storage and Conversion of Henan Province, College of Chemical and Materials Engineering, Institute of Surface Micro and Nano Materials, Xuchang University, Xuchang, Henan 461000, P. R. China.

Nanozymes show great promise in bioanalysis and therapeutics, which largely rely on high catalytic efficiency and selectivity. Inspired by the interfacial electronic interaction between noble metals and transition metal carbides, Pt nanozymes are modified with TiC MXene nanosheets to construct TiC/Pt hybrids with synergistically enhanced catalytic activity. Although TiC does not have oxidase and peroxidase-like activity, it can greatly selectively enhance the peroxidase-like activity of Pt nanozymes. Near-infrared irradiation can further increase specifically the peroxidase-like activity of TiC/Pt. The optimal peroxidase-like activity of TiC/Pt is 6 times higher than Pt in the dark and 7.9 times higher than Pt under illumination. This catalytic enhancement is attributed to the interplay of the strong interfacial electron effect and unique photothermal effect of TiC. Using the superior peroxidase-like activity of TiC/Pt, dual mechanism colorimetric methods based on cascade reaction and inhibitory effect are developed for specific detection of glucose and glutathione with a limit of detection of 1.0 μM and 0.0089 μM, respectively. Our work provides an effective means to improve the catalytic activity and selectivity of nanozymes by introduction of an ideal supporter, which will be of value for the design of efficient nanozymes.
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http://dx.doi.org/10.1039/d2dt01696fDOI Listing
August 2022

Deep Learning Models for Segmenting Non-perfusion Area of Color Fundus Photographs in Patients With Branch Retinal Vein Occlusion.

Front Med (Lausanne) 2022 30;9:794045. Epub 2022 Jun 30.

Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To develop artificial intelligence (AI)-based deep learning (DL) models for automatically detecting the ischemia type and the non-perfusion area (NPA) from color fundus photographs (CFPs) of patients with branch retinal vein occlusion (BRVO).

Methods: This was a retrospective analysis of 274 CFPs from patients diagnosed with BRVO. All DL models were trained using a deep convolutional neural network (CNN) based on 45 degree CFPs covering the fovea and the optic disk. We first trained a DL algorithm to identify BRVO patients with or without the necessity of retinal photocoagulation from 219 CFPs and validated the algorithm on 55 CFPs. Next, we trained another DL algorithm to segment NPA from 104 CFPs and validated it on 29 CFPs, in which the NPA was manually delineated by 3 experienced ophthalmologists according to fundus fluorescein angiography. Both DL models have been cross-validated 5-fold. The recall, precision, accuracy, and area under the curve (AUC) were used to evaluate the DL models in comparison with three types of independent ophthalmologists of different seniority.

Results: In the first DL model, the recall, precision, accuracy, and area under the curve (AUC) were 0.75 ± 0.08, 0.80 ± 0.07, 0.79 ± 0.02, and 0.82 ± 0.03, respectively, for predicting the necessity of laser photocoagulation for BRVO CFPs. The second DL model was able to segment NPA in CFPs of BRVO with an AUC of 0.96 ± 0.02. The recall, precision, and accuracy for segmenting NPA was 0.74 ± 0.05, 0.87 ± 0.02, and 0.89 ± 0.02, respectively. The performance of the second DL model was nearly comparable with the senior doctors and significantly better than the residents.

Conclusion: These results indicate that the DL models can directly identify and segment retinal NPA from the CFPs of patients with BRVO, which can further guide laser photocoagulation. Further research is needed to identify NPA of the peripheral retina in BRVO, or other diseases, such as diabetic retinopathy.
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http://dx.doi.org/10.3389/fmed.2022.794045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279621PMC
June 2022

Efficacy, safety, and tolerability of lebrikizumab in adolescent patients with uncontrolled asthma (ACOUSTICS).

Clin Transl Allergy 2022 Jul 14;12(7):e12176. Epub 2022 Jul 14.

Roche Products Ltd Welwyn Garden City UK.

Background: Lebrikizumab is a monoclonal antibody that modulates activity of interleukin-13. The Phase 3 ACOUSTICS study assessed lebrikizumab efficacy and safety in adolescents with uncontrolled asthma despite standard-of-care treatment.

Methods: Adolescents (aged 12-17 years) with uncontrolled asthma, prebronchodilator forced expiratory volume in 1 s 40%-90% predicted, and stable background therapy were randomised 1:1:1 to receive lebrikizumab 125 or 37.5 mg or placebo subcutaneously once every 4 weeks. Primary efficacy endpoint was asthma exacerbation rate over 52 weeks.

Results: Between August 2013 and July 2016, 579 patients were screened and 346 were randomised; 224 (65%) completed the study with 52 weeks of treatment. Lebrikizumab 125 mg ( = 116) reduced the exacerbation rate at 52 weeks versus placebo ( = 117; adjusted rate ratio [RR] 0.49 [95% CI 0.28-0.83]; 51% rate reduction). Lebrikizumab 37.5 mg ( = 113) was less effective at reducing exacerbations (RR 0.60 [95% CI 0.35-1.03]; 40% rate reduction). In patients with blood eosinophil counts ≥300 cells/μl, both lebrikizumab doses reduced exacerbations (125 mg: RR 0.44 [95% CI 0.21-0.89]; 37.5 mg: 0.42 [95% CI 0.19-0.93]). Treatment-emergent adverse events, serious adverse events, and adverse events leading to study discontinuation occurred in 155 (68%), 7 (3%), and 5 (2%) of 229 patients who received lebrikizumab (both 125 and 37.5 mg doses) and in 72 (62%), 4 (3%), and 1 (1%) of 117 who received placebo, respectively. No deaths occurred.

Conclusion: Lebrikizumab 125 mg reduced asthma exacerbation rates in adolescents with uncontrolled asthma. However, the study was prematurely terminated (sponsor's decision) potentially limiting interpretation of results.

Clinical Trial Registration: NCT01875003 (www.ClinicalTrials.gov).
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http://dx.doi.org/10.1002/clt2.12176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281483PMC
July 2022

Using Human Milk Fortifiers to Improve the Preoperative Nutritional Status of Infants With Non-restricted Ventricular Septal Defect.

Front Pediatr 2022 27;10:900012. Epub 2022 Jun 27.

Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fuzhou, China.

Objective: To explore the effects of human milk fortifier (HMF) on improving the preoperative nutritional status of infants with non-restricted ventricular septal defect (VSD).

Methods: A prospective randomized controlled study was conducted in a provincial hospital in China. Participants were randomly divided into an intervention group ( = 29) and a control group ( = 29). HMFs were added proportionally to the infants' feeds in the intervention group based on breastfeeding status, and the infants in the control group received exclusive breastfeeding as needed. The nutritional status of the two groups was compared 1 month after the intervention.

Results: Compared with the control group, the weight, head circumference, height, albumin level, and prealbumin level of the human milk fortifier group were significantly higher 1 month after the intervention ( < 0.05). The STRONGkids score of the HMF group was significantly lower than that of the non-HMF group ( < 0.05). There was no significant difference in pneumonia, liver insufficiency, feeding intolerance, or jaundice between the two groups.

Conclusion: The addition of HMFs based on the breastfeeding status of infants with non-restricted VSD can improve the preoperative nutritional status and does not increase the incidence of gastrointestinal complications.

Clinical Trial Registration: http://www.chictr.org.cn/index.aspx, identifier: ChiCTR2000041135.
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http://dx.doi.org/10.3389/fped.2022.900012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271765PMC
June 2022

The Efficacy of Minocycline Hydrochloride Combined with Multiple Antibiotic Paste in Elderly Patients with Chronic Periodontitis and Concomitant Pulp Lesions.

Evid Based Complement Alternat Med 2022 4;2022:7604741. Epub 2022 Jul 4.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Objective: To explore the efficacy of Minocycline hydrochloride combined with multiple antibiotic pastes in the treatment of chronic periodontitis with concomitant pulp lesions in elderly patients, as well as to analyze the changes in the levels of inflammatory markers.

Methods: 100 patients suffering from chronic periodontitis with concomitant pulp lesions treated in our hospital from July 2018 to January 2020 were selected as the study subjects, and were randomly assigned to either an experimental group ( = 50) or a control group ( = 50). Patients in the experimental group were treated with Minocycline hydrochloride combined with multiple antibiotic pastes, while those in the control group were treated with Minocycline hydrochloride alone. The treatment efficacy in the two groups was then assessed using such factors as periodontal probing depth, dental plaque index, gingival index, periodontal attachment level, and the presence or absence of adverse effects. We also measured and compared the levels of inflammatory markers such as CRP, TNF-, IL-1, IL-5, IL-6, and others in both groups, before treatment, as well as after a week of treatment.

Results: There was a statistically significant difference in the treatment efficacy between the two groups ( < 0.05), with a higher treatment efficacy seen in the experimental group as compared to the control group. When compared to the control group, the experimental group had lower depths of periodontal probing, lower dental plaque indices, lower gingival indices, lower periodontal attachment levels, as well a significantly lower incidence of adverse reactions ( < 0.05). Before treatment, the levels of inflammatory markers such as CRP, TNF-, IL-1, IL-5, and IL-6 were elevated in both groups. After a week of treatment, there were reductions in the levels of inflammatory markers in both groups, but a more significant reduction was seen in the experimental than in the control group ( < 0.05).

Conclusion: Minocycline hydrochloride combined with various antibiotic pastes is more effective in the treatment of chronic periodontitis with concomitant pulp lesions in elderly patients than monotherapy with Minocycline hydrochloride alone.
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http://dx.doi.org/10.1155/2022/7604741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273348PMC
July 2022

Internally inlaid SaCas9 base editors enable window specific base editing.

Theranostics 2022 6;12(10):4767-4778. Epub 2022 Jun 6.

Laboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan university, Renmin Nanlu 17, Chengdu 610041, Sichuan, China.

Base editors composed of catalytic defective Cas9 and cytosine or adenosine deaminase are powerful tools to convert bases in a genome. However, the fixed and narrow editing window of current base editors has impeded their utility. To increase the scope and diversify the editing patterns is quite necessary. We designed a subset of base editors derived from SaCas9 in which deaminase was inlaid into various locations of the SaCas9 protein. The resulting base editors were characterized with multiple genomic sites and were found to have distinct editing features to the N-terminal SaCas9 CBE (Sa-CBE-N). Among them, Sa-CBE-693, in which a cytosine deaminase was inserted between amino acids 693 and 694, showed an increased editing efficiency and a significantly expanded editing window ranging from bases 2-18. This feature enhanced the editing efficiency of enhancer that contains multiple consensus bases in a 15-bp fragment. Another variant, Sa-CBE-125, displayed backward-shifted editing window, which we showed was particularly powerful in editing cytosines that were accompanied with unintended bystander cytosines at their 5' side. Additionally, these editors showed reduced Cas9 independent DNA off-target editing compared with Sa-CBE-N. Our inlaid base editors improved the targeting scope and diversified the editing pattern.
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http://dx.doi.org/10.7150/thno.70869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254239PMC
June 2022

Motion artifact removal in coronary CT angiography based on generative adversarial networks.

Eur Radiol 2022 Jul 12. Epub 2022 Jul 12.

Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Haining Rd.100, Shanghai, 200080, China.

Objectives: Coronary motion artifacts affect the diagnostic accuracy of coronary CT angiography (CCTA), especially in the mid right coronary artery (mRCA). The purpose is to correct CCTA motion artifacts of the mRCA using a GAN (generative adversarial network).

Methods: We included 313 patients with CCTA scans, who had paired motion-affected and motion-free reference images at different R-R interval phases in the same cardiac cycle and included another 53 CCTA cases with invasive coronary angiography (ICA) comparison. Pix2pix, an image-to-image conversion GAN, was trained by the motion-affected and motion-free reference pairs to generate motion-free images from the motion-affected images. Peak signal-to-noise ratio (PSNR), structural similarity (SSIM), Dice similarity coefficient (DSC), and Hausdorff distance (HD) were calculated to evaluate the image quality of GAN-generated images.

Results: At the image level, the median of PSNR, SSIM, DSC, and HD of GAN-generated images were 26.1 (interquartile: 24.4-27.5), 0.860 (0.830-0.882), 0.783 (0.714-0.825), and 4.47 (3.00-4.47), respectively, significantly better than the motion-affected images (p < 0.001). At the patient level, the image quality results were similar. GAN-generated images improved the motion artifact alleviation score (4 vs. 1, p < 0.001) and overall image quality score (4 vs. 1, p < 0.001) than those of the motion-affected images. In patients with ICA comparison, GAN-generated images achieved accuracy of 81%, 85%, and 70% in identifying no, < 50%, and ≥ 50% stenosis, respectively, higher than 66%, 72%, and 68% for the motion-affected images.

Conclusion: Generative adversarial network-generated CCTA images greatly improved the image quality and diagnostic accuracy compared to motion-affected images.

Key Points: • A generative adversarial network greatly reduced motion artifacts in coronary CT angiography and improved image quality. • GAN-generated images improved diagnosis accuracy of identifying no, < 50%, and ≥ 50% stenosis.
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http://dx.doi.org/10.1007/s00330-022-08971-5DOI Listing
July 2022

Epigenetics in Alzheimer's Disease.

Front Aging Neurosci 2022 23;14:911635. Epub 2022 Jun 23.

Guangxi Key Lab of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin, China.

Alzheimer's disease (AD) is a neurodegenerative disease with unknown pathogenesis and complex pathological manifestations. At present, a large number of studies on targeted drugs for the typical pathological phenomenon of AD (Aβ) have ended in failure. Although there are some drugs on the market that indirectly act on AD, their efficacy is very low and the side effects are substantial, so there is an urgent need to develop a new strategy for the treatment of AD. An increasing number of studies have confirmed epigenetic changes in AD. Although it is not clear whether these epigenetic changes are the cause or result of AD, they provide a new avenue of treatment for medical researchers worldwide. This article summarizes various epigenetic changes in AD, including DNA methylation, histone modification and miRNA, and concludes that epigenetics has great potential as a new target for the treatment of AD.
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http://dx.doi.org/10.3389/fnagi.2022.911635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260511PMC
June 2022

Effect of High-Frequency Oscillatory Ventilation, Combined With Prone Positioning, in Infants With Acute Respiratory Distress Syndrome After Congenital Heart Surgery: A Prospective Randomized Controlled Trial.

J Cardiothorac Vasc Anesth 2022 Jun 18. Epub 2022 Jun 18.

Department of Cardiac Surgery, Fujian Branch of Shanghai Children's Medical Center, Fuzhou, China; Fujian Children's Hospital, Fuzhou, China; Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fuzhou, China. Electronic address:

Objectives: This study aimed to evaluate the effect of high-frequency oscillatory ventilation, (HFOV) combined with prone positioning, on oxygenation and pulmonary ventilation in infants with acute respiratory distress syndrome (ARDS) after congenital heart surgery.

Design: A randomized controlled trial.

Setting: A single-center study at a tertiary teaching hospital.

Participants: Patients with postoperative ARDS after congenital heart disease were divided randomly into the following 2 groups: HFOV combined with prone position (HFOV-PP), and HFOV combined with supine position (HFOV-SP).

Interventions: The primary outcomes were the PaO/FO ratio and the oxygenation index after the intervention, and the secondary outcomes were respiratory variables, hemodynamics, complications, and other short-term outcomes.

Results: Sixty-five eligible infants with ARDS were randomized to either the HFOV-PP (n = 32) or HFOV-SP (n = 33) group. No significant difference in baseline data was found between the 2 groups (p > 0.05). Oxygenation was improved in both groups after HFOV intervention. Compared with the HFOV-SP group, the HFOV-PP group had significantly increased PaO/FO and oxygenation index and a shorter duration of invasive ventilation and length of cardiac intensive care unit stay. No serious complications occurred in the 2 groups.

Conclusion: HFOV-PP significantly improved oxygenation in infants with ARDS after cardiac surgery and had no serious complications.
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http://dx.doi.org/10.1053/j.jvca.2022.06.010DOI Listing
June 2022

Dynamic Tensile Behavior of Woven C/SiC Composite: Experiments and Strain-Rate-Dependent Yield Criterion.

Materials (Basel) 2022 Jul 4;15(13). Epub 2022 Jul 4.

School of Mechanical Engineering, Southeast University, Nanjing 211189, China.

This paper studies the yield behavior of a woven carbon-fiber-reinforced silicon-matrix (C/SiC) composite under dynamic tensile loading. Experiments were carried out to obtain the tensile properties of the C/SiC composite at a strain rate range of 2 × 10/s to 99.4/s. A strain-rate-dependent yield criterion based on the distortional strain energy density theory is established to describe the yield behavior. The interval uncertainty is considered for a more reliable yield prediction. Experimental results show that the yield stress, elastic modulus, and yield strain of the C/SiC composite grow with the increasing strain rate. The failure mode transitions from progressive crack extension to uneven fiber bundle breakage. The predicted results by the yield criterion match well with experimental data. Experimental results are enveloped within the uncertainty level of 45% in the critical distortional energy density, corresponding to an uncertainty of 14% and 11% in the yield stress and yield strain, respectively. With the support of the proposed strain-rate-dependent yield criterion, the yield behavior of the C/SiC composite under dynamic loading conditions can be predicted with reasonable accuracy.
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http://dx.doi.org/10.3390/ma15134679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267425PMC
July 2022

Description of the novel HLA-A allele, HLA-A*02:937 in a Chinese individual.

HLA 2022 Jul 7. Epub 2022 Jul 7.

Clinical Transfusion Research Center, Institute of Blood Transfusion, CAMS & PUMC, Chengdu, China.

HLA-A*02:937 has one nucleotide change compared with HLA-A*02:07:01:01 in codon 182 of exon 3 (ACG>GCG).
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http://dx.doi.org/10.1111/tan.14735DOI Listing
July 2022

Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model.

Redox Biol 2022 08 2;54:102392. Epub 2022 Jul 2.

Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu, China. Electronic address:

Granulocyte-monocyte progenitors (GMPs) differentiate into both neutrophils and monocytes. Recently, uni-potential neutrophil progenitors have been identified both in mice and humans using an array of surface markers. However, how human GMPs commit to neutrophil progenitors and the regulatory mechanisms of fate determination remain incompletely understood. In the present study, we established a human neutrophil deficiency model using the small molecule alpha-lipoic acid. Using this neutrophil deficiency model, we determined that the neutrophil progenitor commitment process from CD371 CD115 GMPs defined by CD34 and CD15 and discovered that critical signals generated by RNA splicing and rRNA biogenesis regulate the process of early commitment for human early neutrophil progenitors derived from CD371 CD115 GMPs. These processes were elucidated by single-cell RNA sequencing both in vitro and in vivo derived cells. Sequentially, we identified that the transcription factor ELK1 is essential for human neutrophil lineage commitment using the alpha-lipoic acid (ALA)-inducing neutrophil deficiency model. Finally, we also revealed differential roles for long-ELK1 and short-ELK1, balanced by SF3B1, in the commitment process of neutrophil progenitors. Taken together, we discovered a novel function of ALA in regulating neutrophil lineage specification and identified that the SF3B1-ELK axis regulates the commitment of human neutrophil progenitors from CD371 CD115 GMPs.
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http://dx.doi.org/10.1016/j.redox.2022.102392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287745PMC
August 2022
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