Publications by authors named "Qian Yang"

1,510 Publications

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Morphological changes of macrophages and their potential contribution to tendon healing.

Colloids Surf B Biointerfaces 2021 Oct 1;209(Pt 1):112145. Epub 2021 Oct 1.

The Nanomedicine Research Laboratory, Research for Frontier Medicine and Hand Surgery Research Center, Research Center of Clinic Medicine, Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China. Electronic address:

Poor healing ability and adhesion formation greatly hinder the recovery of injured tendon function. Previously, our local sustained gene delivery system by using cyclooxygenases (COX-1 and COX-2)-engineered miRNA plasmid/nanoparticles loaded hydrogel significantly inhibited adhesion formation and promoted tendon healing. The present study aims to study morphological changes of the macrophages in the healing tendons after above treatment with the hydrogel. Firstly, we assessed the therapeutic effect of localized delivery of the hydrogel on cyclooxygenases in the injured rat Achilles tendon model. We found ultimate strengths of the healing tendons were significantly increased at week 2 and 3. We then studied the distribution of macrophages before and after tendon injury, and found macrophages were rapidly recruited into injured sites of tendons. After being isolated and cultured, macrophages were transfected with 6-Carboxyfluorescein (FAM) labeled siRNA/nanoparticles and presented a high transfection efficiency (>70%). We further compared the change of iNOS/CD206 in macrophages between negative control siRNA/nanoparticle group and COX siRNA/nanoparticle group. The major finding is that the morphology of the macrophages changed from type I macrophages to type II macrophages after transfection of COX siRNA/nanoparticles in vitro. Subsequently, rat Achilles tendon cells were cultured with supernatant collected from macrophages transfected with negative control siRNA/nanoparticles and COX siRNA/nanoparticles, and the proliferation of tendon cells was significantly increased in COX siRNA/nanoparticle supernatant group. Because type II macrophages are responsible for tissue repair, the changes in macrophage polarization from M1 to M2 may be one of the important events in promoting the tendon healing.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112145DOI Listing
October 2021

Ivabradine Ameliorates Cardiac Function in Heart Failure with Preserved and Reduced Ejection Fraction via Upregulation of miR-133a.

Oxid Med Cell Longev 2021 29;2021:1257283. Epub 2021 Sep 29.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Heart failure (HF) is a clinical syndrome caused by impairment of ventricular filling, ejection of blood, or both and is categorized as HF with reduced ejection fraction (HFrEF) or HF with preserved ejection fraction (HFpEF) based on left ventricular function. Cardiac fibrosis contributes to left ventricular dysfunction and leads to the development of HF. Ivabradine, an If current selective specific inhibitor, has been shown to improve the prognosis of patients with HF. However, the effects of ivabradine on cardiac function and fibrosis in HFpEF and HFrEF and the underlying mechanism remain unclear. In the present study, we utilized mouse models to mimic HFpEF and HFrEF and evaluated the therapeutic effects of ivabradine. By treating mice with different doses (10 mg/kg/d and 20 mg/kg/d) of ivabradine for 4 or 8 weeks, we found that a high dose of ivabradine improved cardiac diastolic function in HFpEF mice and ameliorated cardiac diastolic and systolic function and ventricular tachycardia incidence in HFrEF mice. Moreover, ivabradine significantly reduced the activation of cardiac fibroblasts and myocardial fibrosis in mice. Mechanistically, microRNA-133a, which was upregulated by ivabradine, targeted connective tissue growth factor and collagen 1 in cardiac fibroblasts and might contribute to the protective role of ivabradine. Together, our work utilized mouse models to study HFpEF and HFrEF, demonstrated the protective role of ivabradine in HFpEF and HFrEF, and elucidated the potential underlying mechanism, which provides an effective strategy for related diseases.
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http://dx.doi.org/10.1155/2021/1257283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494584PMC
September 2021

High Proteoglycan Decorin Levels Are Associated With Acute Coronary Syndrome and Provoke an Imbalanced Inflammatory Response.

Front Physiol 2021 21;12:746377. Epub 2021 Sep 21.

Department of Cardiovascular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Acute coronary syndrome (ACS) has become one of the most common causes of disability. It is thus important to identify ACS early in the disease course of patients using novel biomarkers for prompt management. Decorin (DCN) was well-acknowledged for its effect on collagen fibrillogenesis and maintaining tissue integrity. Additionally, DCN could release as secreted proteoglycan under pathological conditions. Hence, we aimed to determine the relationship between serum DCN concentration and ACS. A total of 388 patients who underwent coronary angiography (CAG) in the cardiovascular center of Ruijin Hospital between June 2016 and December 2017 were enrolled in this study. Blood samples were drawn during CAG surgery to determine the serum DCN level of patients with ACS ( = 210) and control subjects ( = 178) using enzyme-linked immunosorbent assay. We found that the serum DCN levels of ACS patients were elevated compared with those of the control subjects (13.59 ± 0.50 vs. 13.17 ± 0.38, respectively, < 0.001). Furthermore, the serum DCN level, after being adjusted with other cardiovascular factors, was independently associated with ACS. Moreover, an increased serum DCN level was positively correlated with the number of white blood cells and the level of high-sensitivity C-reactive protein ( = 0.3 and 0.11, respectively). Mechanistically, DCN might have elicited an imbalanced inflammatory response during cardiac ischemia by suppressing the expression of anti-inflammatory genes. Serum DCN is a novel biomarker of ACS and contributes to the increased inflammatory response in ischemic heart disease.
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http://dx.doi.org/10.3389/fphys.2021.746377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490816PMC
September 2021

Melatonin functions in priming of stomatal immunity in Panax notoginseng and Arabidopsis thaliana.

Plant Physiol 2021 Sep 1. Epub 2021 Sep 1.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.

Melatonin (MT) plays important roles in plant disease response, but the mechanisms are largely unknown. Here, we show that MT functions in stomatal immunity in Panax notoginseng and Arabidopsis thaliana. Biochemical analyses showed that MT-induced stomatal closure plays a prominent role in preventing invasion of bacteria Pseudomonas syringe pv. tomato (Pst) DC3000 via activation of mitogen-activated protein kinase (MAPK) and NADPH oxidase-mediated reactive oxygen species production in P. notoginseng. The first putative phytomelatonin receptor 1 (PMTR1) is a plasma membrane protein required for perceiving MT signaling in stomatal closure and activation of MAPK. Biochemical and genetic tests found PMTR1 is essential for flg22- and MT-induced MAPK activation in a heterotrimeric GTP-binding protein Gα subunit GPA1-independent manner. GPA1 functions in the same genetic pathways of FLS2/BAK1 (Flagellin Sensing 2/Brassinosteroid Insensitive 1-associated kinase 1)- as well as PMTR1-mediated flg22 and MT signaling in stomatal closure. The stomata in pmtr1 are insensitive to MT and flg22, but the application of MT induces stomatal closure and reduces the bacterial growth in fls2 and bak1 plants, indicating that PMTR1 might be a downstream signaling component in FLS2- and BAK1-mediated stomatal immunity. In summary, our results (i) demonstrate that phytomelatonin functions in the priming of stomatal immunity and (ii) provide insights into the phytomelatonin signaling transduction pathway.
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http://dx.doi.org/10.1093/plphys/kiab419DOI Listing
September 2021

Dual-depth augmented reality display with reflective polarization-dependent lenses.

Opt Express 2021 Sep;29(20):31478-31487

Vergence-accommodation conflict (VAC) is a common annoying issue in near-eye displays using stereoscopy technology to provide the perception of three-dimensional (3D) depth. By generating multiple image planes, the depth cues can be corrected to accommodate a comfortable 3D viewing experience. In this study, we propose a multi-plane optical see-through augmented reality (AR) display with customized reflective polarization-dependent lenses (PDLs). Leveraging the different optical powers of two PDLs, a proof-of-concept dual-plane AR device is realized. The proposed design paves the way to a compact, lightweight, and fatigue-free AR display.
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http://dx.doi.org/10.1364/OE.435914DOI Listing
September 2021

Correction: NocU is a cytochrome P450 oxygenase catalyzing -hydroxylation of the indolic moiety during the maturation of the thiopeptide antibiotics nocathiacins.

Org Biomol Chem 2021 Oct 14;19(39):8613. Epub 2021 Oct 14.

State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence on Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.

Correction for 'NocU is a cytochrome P450 oxygenase catalyzing -hydroxylation of the indolic moiety during the maturation of the thiopeptide antibiotics nocathiacins' by Heng Guo , , 2021, DOI: 10.1039/d1ob01284c.
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http://dx.doi.org/10.1039/d1ob90138aDOI Listing
October 2021

COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products - Liaoning Province, China, July 2020.

China CDC Wkly 2021 May;3(21):441-447

China National Center for Food Safety Risk Assessment, Beijing, China.

Few major outbreaks of coronavirus disease 2019 (COVID-19) have occurred in China after major non-pharmaceutical interventions and vaccines have been deployed and implemented. However, sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..



In July 2020, a COVID-19 outbreak occurred in Dalian, China. The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.



Virus contaminated paper surfaces could maintain infectivity for at least 17-24 days at -25 ℃. Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population. Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods. Several measures to prevent the introduction of the virus via cold-chain goods can be implemented.
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http://dx.doi.org/10.46234/ccdcw2021.114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392985PMC
May 2021

The impact of health education videos on general public's mental health and behavior during COVID-19.

Glob Health Res Policy 2021 09 30;6(1):37. Epub 2021 Sep 30.

Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.

Background: COVID-19 has seriously affected people's mental health and changed their behaviors. Previous studies for mental state and behavior promotion only targeted limited people or were not suitable for daily activity restrictions. Therefore, we decided to explore the effect of health education videos on people's mental state and health-related behaviors.

Methods: Based on WeChat, QQ, and other social media, we conducted an online survey by snowball sampling. Spearman's non-parametric method was used to analyze the correlation related to mental health problems and health-related behaviors. Besides, we used binary logistic regression analyses to examine mental health problems and health-related behaviors' predictors. We performed SPSS macro PROCESS (model 4 and model 6) to analyze mediation relationships between exposure to health education videos and depression/anxiety/health-related behaviors. These models were regarded as exploratory.

Results: Binary logistic regression analyses indicated that people who watched the health education videos were more likely to wear masks (OR 1.15, p < 0.001), disinfect (OR 1.26, p < 0.001), and take temperature (OR 1.37, p < 0.001). With higher level of posttraumatic growth (PTG) or perceived social support (PSS), people had lower percentage of depression (For PSS, OR 0.98, p < 0.001; For PTG, OR 0.98, p < 0.01) and anxiety (For PSS, OR 0.98, p < 0.001; For PTG, OR 0.98, p = 0.01) and better health behaviors. The serial multiple-mediation model supported the positive indirect effects of exposure to health education videos on the depression and three health-related behaviors through PSS and PTG (Depression: B[SE] =  - 0.0046 [0.0021], 95% CI - 0.0098, - 0.0012; Mask-wearing: B[SE] = 0.0051 [0.0023], 95% CI 0.0015, 0.0010; Disinfection: B[SE] = 0.0059 [0.0024], 95% CI 0.0024, 0.0012; Temperature-taking: B[SE] = 0.0067 [0.0026], 95% CI 0.0023, 0.0013).

Conclusion: Exposure to health education videos can improve people's self-perceived social support and inner growth and help them cope with the adverse impact of public health emergencies with better mental health and health-related behaviors.
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http://dx.doi.org/10.1186/s41256-021-00211-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482355PMC
September 2021

MNSFβ Regulates TNFα Production by Interacting with RC3H1 in Human Macrophages, and Dysfunction of MNSFβ in Decidual Macrophages Is Associated With Recurrent Pregnancy Loss.

Front Immunol 2021 13;12:691908. Epub 2021 Sep 13.

National Health Commission (NHC) of the People's Republic of China Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Pharmacy, Fudan University, Shanghai, China.

Decidual macrophages (dMϕ) are the second largest population of leukocytes at the maternal-fetal interface and play critical roles in maintaining pregnancy. Our previous studies demonstrated the active involvement of monoclonal nonspecific suppressor factor-β (MNSFβ) in embryonic implantation and pregnancy success. MNSFβ is a ubiquitously expressed ubiquitin-like protein that also exhibits immune regulatory potential, but its function in human dMϕ remains unknown. Here, we observed that the proportion of CD11c (CD11cHI) dMϕ was significantly increased in dMϕ derived from patients with recurrent pregnancy loss (RPL dMϕ) compared to those derived from normal pregnant women (Control dMϕ). The production of MNSFβ and TNFα by RPL dMϕ was also significantly increased compared to that by Control dMϕ. Conditioned medium from RPL dMϕ exerted an inhibitory effect on the invasiveness of human trophoblastic HTR8/SVneo cells, and this effect could be partially reversed by a neutralizing antibody against TNFα. Bioinformatics analysis indicated a potential interaction between MNSFβ and RC3H1, a suppressor of TNFα transcription. Immunoprecipitation experiments with human Mϕ differentiated from the human monocyte cell line Thp1 (Thp1-derived Mϕ) proved the binding of MNSFβ to RC3H1. Specific knockdown of MNSFβ in Thp1-derived Mϕ led to a marked decrease in TNFα production, which could be reversed by inhibiting RC3H1 expression. Interestingly, a significant decrease in the protein level of RC3H1 was observed in RPL dMϕ. Together, our findings indicate that aberrantly increased MNSFβ expression in dMϕ may promote TNFα production its interaction with RC3H1, and these phenomena could result in the disruption of the immune balance at the maternal-fetal interface and thus pregnancy loss.
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http://dx.doi.org/10.3389/fimmu.2021.691908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473736PMC
September 2021

Changes in serum TG levels during pregnancy and their association with postpartum hypertriglyceridemia: a population-based prospective cohort study.

Lipids Health Dis 2021 Sep 29;20(1):119. Epub 2021 Sep 29.

Department of Nutrition and Food Hygiene, School of Public Health, Capital Medical University, 100069, Beijing, P.R. China.

Background: Blood lipid increases during gestation are considered a physiological adaption, and decrease after delivery. However, some adverse pregnancy outcomes are thought to be related to gestational lipid levels. Therefore, it is necessary to have a reference range for lipid changes during gestation. The present study aims to describe triglyceride (TG) changes during pregnancy and 42 days postpartum and to find cut-off points for TG levels during the first, second, and third trimesters.

Methods: A total of 908 pregnant women were followed from recruitment to 42 days postpartum, and their serum lipids were collected at gestational weeks 6-8, 16, 24, and 36 and 42 days postpartum. The major outcome was postpartum hypertriglyceridemia. The association between gestational and postpartum TG levels was analysed by stepwise multiple linear regression. A two-stage approach including a linear mixed-effect model and linear or logistic regression was conducted to explore the contribution of the changes in TG over time in pregnancy to postpartum hypertriglyceridemia. Logistic regression was constructed to examine the association between gestational TG levels and postpartum hypertriglyceridemia. Cut-off points were calculated by receiver operating characteristic (ROC) curves.

Results: There was a tendency for serum TG to increase with gestational age and decrease at 42 days postpartum. Prepregnancy overweight, obesity, and GDM intensified this elevation. Higher TG levels at gestational weeks 6-8, 16, 24, and 36 were positively associated with a higher risk of postpartum hypertriglyceridemia [OR 4.962, 95 % CI (3.007-8.189); OR 2.076, 95 % CI (1.303-3.309); OR 1.563, 95 % CI (1.092-2.236); and OR 1.534, 95 % CI (1.208-1.946), respectively]. The trend of the change in TG over time was positively associated with the TG level and risk of postpartum hypertriglyceridemia [OR 11.660, 95 % CI (6.018-22.591)]. Based on ROC curves, the cut-off points of serum TG levels were 1.93, 2.35, and 3.08 mmol/L at gestational weeks 16, 24, and 36, respectively. Stratified analysis of prepregnancy body mass index (pre-BMI) and GDM showed that higher gestational TG was a risk factor for postpartum hypertriglyceridemia in women with normal pre-BMI and without GDM.

Conclusions: Gestational TG and its elevation were risk and predictive factors of postpartum hypertriglyceridemia, especially in pregnant women with normal pre-BMI or without GDM.
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http://dx.doi.org/10.1186/s12944-021-01549-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480071PMC
September 2021

Inhibition of organosilane/[email protected] self-healing passivator for pyrite oxidation.

Chemosphere 2021 Sep 23;287(Pt 3):132342. Epub 2021 Sep 23.

Guangdong Provincial Key Lab of Green Chemical Product Technology, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, PR China. Electronic address:

Organosilane, with functional organic groups attached to inorganic silicon atoms, exhibits excellent passivation performance for pyrite. However, a considerable number of micro-cracks will gradually appear on the surface of passivation film under long-term corrosion of oxidizing medium, resulting in a significant decrease of passivation effect. To improve the stability and long-term performance of organosilane coating, a novel passivator ([email protected]) with self-healing function was prepared to inhibit the oxidation of pyrite. We chose 3-mercaptopropyltrimethoxysilane (Prop-SH) and tetraethoxysilane (TEOS) as the host coating (PT), and attapulgite clay (ATP) loaded with 8-hydroxyquinoline (8-HQ) was used to endow the coating with better passivation and self-healing performance. The electrochemical and chemical leaching results showed that the addition of [email protected] greatly improved the passivation performance of PT coating. The passivation efficiencies of total Fe and SO reached to 88.1% and 79.2%, respectively. We also found that the protective capability of the scratched [email protected] coating can be recovered automatically through 8-HQ release from ATP. The passivation and self-healing mechanisms were investigated by FT-IR, XPS, Si NMR, and other characterization methods, which were as follows: firstly, the organosilanes hydrolyzed to form highly active silanol groups, then dehydration condensation reaction occurred between silanol molecules and [email protected] to obtain cross-linked network structure connected by Si-O-Si bonds. After that, Si-OH groups reacted with the hydroxyl groups of pyrite to form Fe-O-Si bonds, thereby an inert and dense passivation film attached to the surface of pyrite. Once the passivation film is locally damaged, 8-HQ will automatically release to repair the cracks.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132342DOI Listing
September 2021

Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors.

J Exp Clin Cancer Res 2021 Sep 27;40(1):303. Epub 2021 Sep 27.

Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.

Methods: We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au [email protected] are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer.

Results: The results display that Au [email protected] can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection.

Conclusion: Our findings demonstrate that the synthesized Au [email protected] offer a facile platform to exert antitumor and improve the diagnostic level of tumor.
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http://dx.doi.org/10.1186/s13046-021-02105-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477545PMC
September 2021

Iterative Upgrading of Small Molecular Tyrosine Kinase Inhibitors for EGFR Mutation in NSCLC: Necessity and Perspective.

Pharmaceutics 2021 Sep 18;13(9). Epub 2021 Sep 18.

Respiratory and Critical Care Medicine, Mianyang Central Hospital, Mianyang 621000, China.

Molecular targeted therapy has been reported to have fewer adverse effects, and offer a more convenient route of administration, compared with conventional chemotherapy. With the development of sequencing technology, and research on the molecular biology of lung cancer, especially whole-genome information on non-small cell lung cancer (NSCLC), various therapeutic targets have been unveiled. Among the NSCLC-driving gene mutations, epidermal growth factor receptor (EGFR) mutations are the most common, and approximately 10% of Caucasian, and more than 50% of Asian, NSCLC patients have been found to have sensitive EGFR mutations. A variety of targeted therapeutic agents for EGFR mutations have been approved for clinical applications, or are undergoing clinical trials around the world. This review focuses on: the indications of approved small molecular kinase inhibitors for EGFR mutation-positive NSCLC; the mechanisms of drug resistance and the corresponding therapeutic strategies; the principles of reasonable and precision molecular structure; and the drug development discoveries of next-generation inhibitors for EGFR.
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http://dx.doi.org/10.3390/pharmaceutics13091500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468657PMC
September 2021

Spore-Trained Dendritic Cells Enhance the Generation of Memory T Cells via ICAM1.

Cells 2021 Aug 31;10(9). Epub 2021 Aug 31.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.

Immunological memory is a cardinal feature of the immune system. The intestinal mucosa is the primary exposure and entry site of infectious organisms. For an effective and long-lasting safeguard, a robust immune memory system is required, especially by the mucosal immunity. It is well known that tissue-resident memory T cells (Trms) provide a first response against infections reencountered at mucosal tissues surfaces, where they accelerate pathogen clearance. However, their function in intestinal immunization remains to be investigated. Here, we report enhanced local mucosal and systemic immune responses through oral administration of H9N2 influenza whole inactivated virus (H9N2 WIV) plus spores. Subsequently, H9N2 WIV plus spores led to the generation of CD103 CD69 Trms, which were independent of circulating T cells during the immune period. Meanwhile, we also found that spores could stimulate Acrp30 expression in 3T3-L1 adipocytes. Moreover, spore-stimulated adipocyte supernatant also upregulated the expression of intercellular adhesion molecule-1 (ICAM1) in dendritic cells (DCs). Furthermore, the proportion of HA-tetramer cells was severely curtailed upon suppressed ICAM1 expression, which also depended on HA-loaded DCs. Taken together, our data demonstrated that spore-promoted H9N2 WIV induced an immune response by enhancing Trms populations, which were associated with the activation of ICAM1 in DCs.
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http://dx.doi.org/10.3390/cells10092267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469252PMC
August 2021

Identification and Evaluation of Autoantibody to a Novel Tumor-Associated Antigen GNA11 as a Biomarker in Esophageal Squamous Cell Carcinoma.

Front Oncol 2021 10;11:661043. Epub 2021 Sep 10.

Henan Institute of Medical and Pharmaceutical Sciences in Academy of Medical Science, Zhengzhou University, Zhengzhou, China.

The study aims to explore the diagnostic value of anti-GNA11 autoantibody in esophageal squamous cell carcinoma (ESCC) from multiple levels. Autoantibody against GNA11 with the highest diagnostic performance was screened out from the customized protein microarray. A total of 486 subjects including ESCC patients and matched normal controls were recruited in the verification and validation phases by using enzyme-linked immunosorbent assay (ELISA). Western blotting analysis was used to verify the ELISA results. Immunohistochemistry (IHC) was used to evaluate GNA11 expression in ESCC tissues and para-tumor tissues. In addition, a bioinformatics approach was adopted to investigate the mRNA expression of GNA11 in ESCC. Results indicated that the level of anti-GNA11 autoantibody in ESCC patients was significantly higher than that in the normal controls, and it can be used to distinguish ESCC patients from normal individuals in clinical subgroups ( < 0.05), as revealed by both ELISA and Western blotting. The receiver operating characteristic (ROC) curve analysis showed that anti-GNA11 autoantibody could distinguish ESCC patients from normal controls with an area under the ROC curve (AUC) of 0.653, sensitivity of 10.96%, and specificity of 98.63% in the verification cohort and with an AUC of 0.751, sensitivity of 38.24%, and specificity of 88.82% in the validation cohort. IHC manifested that the expression of GNA11 can differentiate ESCC tissues with para-tumor tissues ( < 0.05), but it cannot be used to differentiate different pathological grades and clinical stages ( > 0.05). The mRNA expression of GNA11 in ESCC patients and normal controls was different with a bioinformatics mining with The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data in Gene Expression Profiling Interactive Analysis (GEPIA). In summary, anti-GNA11 autoantibody has the potential to be a new serological marker in the diagnosis of ESCC.
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http://dx.doi.org/10.3389/fonc.2021.661043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462091PMC
September 2021

The Positive Role and Mechanism of Herbal Medicine in Parkinson's Disease.

Oxid Med Cell Longev 2021 3;2021:9923331. Epub 2021 Sep 3.

Institute of Pediatrics, Department of Neonatology, Developmental and Behavioral Pediatric Department & Child Health Care Department, Children's Hospital of Fudan University, Fudan University, Shanghai, China.

Parkinson's disease (PD) is a complex neurodegenerative disease, manifested by the progressive functional impairment of the midbrain nigral dopaminergic neurons. Due to the unclear underlying pathogenesis, disease-modifying drugs for PD remain elusive. In Asia, such as in China and India, herbal medicines have been used in the treatment of neurodegenerative disease for thousands of years, which recently attracted considerable attention because of the development of curative drugs for PD. In this review, we first summarized the pathogenic factors of PD including protein aggregation, mitochondrial dysfunction, ion accumulation, neuroinflammation, and oxidative stress, and the related recent advances. Secondly, we summarized 32 Chinese herbal medicines (belonging to 24 genera, such as , , and ), 22 Chinese traditional herbal formulations, and 3 Indian herbal medicines, of which the ethanol/water extraction or main bioactive compounds have been extensively investigated on PD models both and . We elaborately provided pictures of the representative herbs and the structural formula of the bioactive components (such as leutheroside B and astragaloside IV) of the herbal medicines. Also, we specified the potential targets of the bioactive compounds or extractions of herbs in view of the signaling pathways such as PI3K, NF-B, and AMPK which are implicated in oxidative and inflammatory stress in neurons. We consider that this knowledge of herbal medicines or their bioactive components can be favorable for the development of disease-modifying drugs for PD.
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http://dx.doi.org/10.1155/2021/9923331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457986PMC
September 2021

In silico species identification and serotyping for Cronobacter isolates by use of whole-genome sequencing data.

Int J Food Microbiol 2021 Nov 20;358:109405. Epub 2021 Sep 20.

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, People's Republic of China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, People's Republic of China; Center for Microbial Functional Genomics and Detection Technology, Ministry of Education, Tianjin, People's Republic of China. Electronic address:

Cronobacter spp. are foodborne pathogens that can cause severe infections in neonates through contaminated powdered infant formula. Accurate and rapid pathogen identification and serotyping are crucial to limit the detrimental effects of bacterial infections, and to prevent outbreaks and sporadic infections. Conventional serotyping is tedious, laborious, and time-consuming; however, with whole-genome sequencing (WGS) becoming faster and cheaper, WGS has vast potential in routine typing and surveillance. Hence, in this study, we developed a publicly available tool, CroTrait (CronobacterTraits), for in silico species identification and O serotyping of Cronobacter isolates based on WGS data. CroTrait showed excellent performance in species identification and O serotyping when 810 genomes with known species identities and 276 genomes with known O serotype were tested. Moreover, CroTrait allows rapid prediction of new potential O serotypes. We identified 11 novel potential O serotypes of Cronobacter using CroTrait. Therefore, CroTrait is a convenient and promising tool for species identification and O serotyping of Cronobacter isolates.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109405DOI Listing
November 2021

CgIL17-5 regulates the mRNA expressions of immune effectors through inducing the phosphorylation of CgMAPKs and the nuclear translocation of CgRel and CgAP-1 in the Pacific oyster Crassostrea gigas.

Dev Comp Immunol 2021 Sep 23;127:104263. Epub 2021 Sep 23.

Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Control, Dalian Ocean University, Dalian, 116023, China. Electronic address:

Interleukin-17 (IL-17) is a classic pro-inflammatory cytokine that plays an important role in the immune and inflammatory response. In the present study, the sequence feature of CgIL17-5 and its function as a pro-inflammatory factor in inducing the mRNA expressions of downstream immune effectors were investigated in oyster Crassostrea gigas. There were two tightly folded alpha helixes and two pairs of antiparallel beta-pleated sheet in the amino acid sequence of CgIL17-5. The mRNA transcripts of CgIL17-5 were constitutively distributed in all the tested tissues, with the highest level in haemocytes. The mRNA expression level of CgIL17-5 in haemocytes increased significantly at 24 h after Vibrio splendidus stimulation. CgIL17-5 protein was mainly detected in granulocytes which were the main immunocompetent haemocytes in C. gigas. The phosphorylation of mitogen-activated protein kinases (CgJNK, CgERK and CgP38) and nuclear translocation of the transcription factors (CgRel and CgAP-1) in haemocytes were induced after the oysters received an injection of recombinant CgIL17-5 for 2 h. The mRNA expression levels of CgIL-17s, CgTNF-1, Cgdefh1 and Cgdefh2 increased significantly in haemocytes. At the same time, obvious branchial swelling and cilium shedding in gills were observed at 24 h after the oysters received an injection of rCgIL17-5. All the results collectively suggested that CgIL17-5 promoted the activation of CgMAPKs and the nuclear translocation of CgRel and CgAP-1 to promote the mRNA expressions of cytokines and antibacterial peptides.
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http://dx.doi.org/10.1016/j.dci.2021.104263DOI Listing
September 2021

Alteration of the Intestinal Microbial Flora and the Serum IL-17 Level in Patients with Graves' Disease Complicated with Vitamin D Deficiency.

Int Arch Allergy Immunol 2021 Sep 20:1-10. Epub 2021 Sep 20.

Department of Endocrinology, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China.

Background: Intestinal flora is associated with Graves' disease (GD). This study explored the association of serum 25(OH)D with the diversity of the intestinal flora and serum IL-17 in GD patients.

Methods: Patients newly diagnosed with GD at 2 centers between 2018 and 2021 were consecutively included. According to their 25(OH)D levels, they were divided into the deficiency group, the insufficiency group, and the sufficiency group. Some patients with vitamin D deficiency or insufficiency were randomly selected and were matched with healthy volunteers (normal control [NC]) in terms of sex, age, and case number. The diversity and differential species of the intestinal flora and serum IL-17 levels were compared.

Results: Serum 25(OH)D negatively correlated with serum IL-17, the platelet/lymphocyte ratio, and TSH receptor antibody. The diversity of the intestinal flora decreased in the GD group, with noticeable differences in the composition of the intestinal flora when compared with the NC group. At the phylum level, the GD group exhibited a significantly lower abundance of Firmicutes but a higher abundance of Actinobacteria. At the genus level, the GD group exhibited higher relative abundances of Bifidobacterium, Collinsella, and Pediococcus but lower abundances of Roseburia and Dialister.

Conclusions: The changes in the vitamin D level and the composition of the intestinal flora may partially contribute to the development of GD.
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http://dx.doi.org/10.1159/000518949DOI Listing
September 2021

Significance of amplitude integrated electroencephalography in early stage of neonatal hypoxic-ischemic encephalopathy and cerebral function monitoring in Neonatal Intensive Care Units.

Am J Transl Res 2021 15;13(8):9437-9443. Epub 2021 Aug 15.

Department of Pediatrics, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture Enshi 445000, Hubei Province, China.

Objective: To investigate the role of amplitude integrated electroencephalography (aEEG) diagnosis in early stage of neonatal hypoxic-ischemic encephalopathy (HIE), and to evaluate the feasibility of aEEG in cerebral function monitoring in Neonatal Intensive Care Units (NICU).

Methods: 60 cases of term infants with neonatal HIE were included in the observation group, and 50 healthy term infants were enrolled as the control group. Both groups received aEEG monitoring within 6 hours after birth, and the results were analyzed.

Results: The correlation coefficient between the degree of asphyxia, SWC, SA and aEEG background activity was r = 0.571 (<0.001); r = 0.512 (<0.001) and r = 0.293 (<0.001), respectively. The correlation coefficient between HIE degree and aEEG background activity, SWC was r = 0.742 (<0.001) and r = 0.763 (<0.001), respectively. The Gessell scores of the control group at 1, 3, 6, 9, and 12 months after birth were higher than those of the mild asphyxia group and the severe asphyxia group, and the mild asphyxia group showed higher Gessell scores than the severe asphyxia group (<0.001). The predicted ROC curve of aEEG monitoring on the occurrence of neonatal HIE showed the area under the curve (AUC) = 0.6354, Std. Error = 0.05668 (95% CI: 0.5243-0.7465, = 0.0209).

Conclusion: aEEG had obvious diagnostic value in brain injury in the early stage of full-term neonates with asphyxia, and could be used to monitor the cerebral function of NICU, which is helpful for early clinical detection of brain injury of full-term neonates with asphyxia, so as to improve early diagnosis and treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430076PMC
August 2021

LXA4 protects against blue-light induced retinal degeneration in human A2E-laden RPE cells and Balb-c mice.

Ann Transl Med 2021 Aug;9(15):1249

Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.

Background: Age-related macular degeneration (AMD) is one of the leading causes of permanent visual impairment in the elderly. Blue light (BL) has been reported to cause retinal damage and contribute to the onset and development of severe AMD. N-retinylidene-N-retinylethanolamine (A2E), a lipofuscin fluorophore, accumulates with ageing in the retinal pigment epithelium (RPE) cells. Once exposed to BL, A2E easily oxidizes to A2E-epoxides, causing oxidative-stress injury to the retina. Lipoxin A4 (LXA4), an endogenous anti-antioxidant lipid, plays a key role in multiple organs by binding to the formyl-peptide receptor-like 1 (FPRL1). This study examined the protective effects of LXA4 on oxidative-stress injury induced by BL exposure, and clarified the underlying mechanisms in cultured RPE cells and Balb-c mice.

Methods: LXA4 diluent was orally administered to mice before retinal degeneration was established. Optical coherence tomography, retinal histology, and RPE cell injury were assessed.

Results: LXA4 administration significantly ameliorated retinal damage as evidenced by the thicknesses of the retinal layers and the tight junctions of RPE cells . LXA4 inhibited BL-induced reactive oxygen species (ROS) production, reduced tight junctions, and the death of A2E-laden RPE cells. LXA4 also potently increased the expression of haem oxygenase-1 (HO1) and NAD(P)H quinone oxidoreductase 1 (NQO1), probably by decreasing the association between nuclear factor erythroid 2-related factor 2 (NRF2) and Kelch-like ECH (Epichlorohydrin) -associated protein 1 (Keap1), and ameliorating NRF2 nuclear translocation and the antioxidant response element (ARE) deoxyribonucleic acid (DNA) binding activity.

Conclusions: Our results showed that LXA4 ameliorated retinal degeneration, and should be considered in the prevention and treatment of AMD.
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http://dx.doi.org/10.21037/atm-21-3390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421929PMC
August 2021

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line Originated From in .

Front Plant Sci 2021 31;12:721980. Epub 2021 Aug 31.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Crosses that lead to heterosis have been widely used in the rapeseed ( L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility () systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F population developed by crossing the CMS line with its restorer line . Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F buds, respectively, we identified a 6.25 Mb candidate interval where is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat () genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin-proteasome pathway was enriched. The top four hub genes in the protein-protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by and improve our understanding of fertility restoration.
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http://dx.doi.org/10.3389/fpls.2021.721980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438535PMC
August 2021

p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease.

Aging Cell 2021 Oct 16;20(10):e13434. Epub 2021 Sep 16.

Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia, USA.

MicroRNAs (miRNAs) are small noncoding RNAs ubiquitously expressed in the brain and regulate gene expression at the post-transcriptional level. The nuclear RNase III enzyme Drosha initiates the maturation process of miRNAs in the nucleus. Strong evidence suggests that dysregulation of miRNAs is involved in many neurological disorders including Alzheimer's disease (AD). Dysfunction of miRNA biogenesis components may be involved in the processes of those diseases. However, the role of Drosha in AD remains unknown. By using immunohistochemistry, biochemistry, and subcellular fractionation methods, we show here that the level of Drosha protein was significantly lower in the postmortem brain of human AD patients as well as in the transgenic rat model of AD. Interestingly, Drosha level was specifically reduced in neurons of the cortex and hippocampus but not in the cerebellum in the AD brain samples. In primary cortical neurons, amyloid-beta (Aβ) oligomers caused a p38 MAPK-dependent phosphorylation of Drosha, leading to its redistribution from the nucleus to the cytoplasm and a decrease in its level. This loss of Drosha function preceded Aβ-induced neuronal death. Importantly, inhibition of p38 MAPK activity or overexpression of Drosha protected neurons from Aβ oligomers-induced apoptosis. Taken together, these results establish a role for p38 MAPK-Drosha pathway in modulating neuronal viability under Aβ oligomers stress condition and implicate loss of Drosha as a key molecular change in the pathogenesis of AD.
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http://dx.doi.org/10.1111/acel.13434DOI Listing
October 2021

NocU is a cytochrome P450 oxygenase catalyzing -hydroxylation of the indolic moiety during the maturation of the thiopeptide antibiotics nocathiacins.

Org Biomol Chem 2021 Oct 6;19(38):8338-8342. Epub 2021 Oct 6.

State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence on Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.

The ribosomally synthesized and post-translationally modified peptide (RiPP) natural products include the family of thiopeptide antibiotics, where nocathiacins (NOCs) and nosiheptide (NOS) are structurally related bicyclic members featuring an indolic moiety within the side ring system. Compared with NOS, NOCs bear additional functionalities that lead to the improvement of water solubility and bioavailability, a problem inherent to most of the thiopeptide antibiotics, and thus hold potential for clinical use in anti-infective agent development. The process through which post-translational modifications (PTMs) occur to afford these functionalities remains unclear. In this study, an engineered NOS-producing strain is applied to study the function of NocU, a cytochrome P450 oxygenase unique during the PTMs in NOC biosynthesis. Benefiting from the isolation and structure characterization of nosiheptide U (NOS-U), a new NOS-type compound with an extra hydroxyl group at the indole nitrogen, we report that NocU is responsible for the -hydroxylation of the indolic moiety during the maturation of NOCs. This finding reveals the cause of structural differences at the indole nitrogen of NOCs, which will not only accelerate the biosynthetic studies of NOCs, but also promote new analog development by utilizing the compatibility of the biosynthetic machinery of thiopeptide antibiotics.
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http://dx.doi.org/10.1039/d1ob01284cDOI Listing
October 2021

Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.

Virulence 2021 12;12(1):2352-2365

Moe Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, PR China.

Porcine epidemic diarrhea virus (PEDV) is emerging as a major threat to the global swine industry. Clinical PEDV infection is associated with severe intestinal lesions, resulting in absorptive dysfunction and high mortality rates in suckling piglets. The extracellular matrix (ECM) is an important component of intestinal tissue, providing a structural framework and conveying tissue-specific signals to nearby enterocytes. In this study, we investigated the extensive ECM remodeling observed in intestinal epithelial cells infected with PEDV and elucidated the associated activated ECM receptor-related pathways. Protein-protein interaction network analysis revealed two significantly differentially expressed genes (cluster of differentiation 44 [] and serpin family E member 1 []) associated with the ECM. At the transcriptional level, both genes exhibited significant positive correlation with the extent of PEDV replication. Similarly, the expression of CD44 and PAI-1 (encoded by ) was also increased in the intestines of piglets during viral infection. Furthermore, CD44 exhibited antiviral activity by enhancing the expression of antiviral cytokines (e.g., interleukin [IL]-6, IL-18, IL-11, and antimicrobial peptide beta-defensin 1) by activating nuclear factor-κB signaling. Conversely, PAI-1 was found to promote the release of progeny virions during PEDV infection, despite a decreased intracellular viral load. Nevertheless, the underlying mechanisms are still unclear. Taken together, our results highlighted the biological roles of specific ECM-regulated genes, i.e., and in suppressing and promoting PEDV infection, thereby providing a theoretical foundation for the role of the ECM in intestinal infections and identifying potential therapeutic targets for PEDV.
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http://dx.doi.org/10.1080/21505594.2021.1972202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451458PMC
December 2021

Rare sugars: Metabolic Impacts and Mechanisms of Action - a Scoping Review.

Br J Nutr 2021 Sep 10:1-77. Epub 2021 Sep 10.

Division of Food, Nutrition and Dietetics, School of Biosciences, University of Nottingham Sutton Bonington campus, Loughborough LE12 5RD, UK.

Food manufacturers are under increasing pressure to limit the amount of free sugars in their products. Many have reformulated products to replace sucrose, glucose and fructose with alternative sweeteners, but some of these have been associated with additional health concerns. Rare sugars are "monosaccharides and their derivatives that hardly exist in nature", and there is increasing evidence that they could have health benefits. This review aimed to scope the existing literature in order to identify the most commonly researched rare sugars, to ascertain their proposed health benefits, mechanisms of action and potential uses, and to highlight knowledge gaps. A process of iterative database searching identified 55 relevant articles. The reported effects of rare sugars were noted, along with details of the research methodologies conducted. Our results indicated that the most common rare sugars investigated are D-psicose and D-tagatose, with the potential health benefits divided into three topics: glycaemic control, body composition and cardiovascular disease. All the rare sugars investigated have the potential to suppress postprandial elevation of blood glucose and improve glycaemic control in both human and animal models. Some animal studies have suggested that certain rare sugars may also improve lipid profiles, alter the gut microbiome and reduce pro-inflammatory cytokine expression. The present review demonstrates that rare sugars could play a role in reducing the development of obesity, type 2 diabetes, and/or cardiovascular disease. However, understanding of the mechanisms by which rare sugars may exert their effects is limited, and their effectiveness when used in reformulated products is unknown.
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http://dx.doi.org/10.1017/S0007114521003524DOI Listing
September 2021

Chirped dissipative solitons in driven optical resonators.

Optica 2021 Jun;8(6):861-869

Institute of Optics, University of Rochester, Rochester, New York 14627, USA.

Solitons are self-sustaining particle-like wave packets found throughout nature. Optical systems such as optical fibers and mode-locked lasers are relatively simple, are technologically important, and continue to play a major role in our understanding of the rich nonlinear dynamics of solitons. Here we present theoretical and experimental observations of a new class of optical soliton characterized by pulses with large and positive chirp in normal dispersion resonators with strong spectral filtering. Numerical simulations reveal several stable waveforms including dissipative solitons characterized by large frequency chirp. In experiments with fiber cavities driven with nanosecond pulses, chirped dissipative solitons matching predictions are observed. Remarkably, chirped pulses remain stable in low quality-factor resonators despite large dissipation, which enables new opportunities for nonlinear pattern formation. By extending pulse generation to normal dispersion systems and supporting higher pulse energies, chirped dissipative solitons will enable ultrashort pulse and frequency comb sources that are simpler and more effective for spectroscopy, communications, and metrology. Scaling laws are derived to provide simple design guidelines for generating chirped dissipative solitons in microresonator, fiber resonator, and bulk enhancement cavity platforms.
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http://dx.doi.org/10.1364/optica.419771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425384PMC
June 2021

The Middle Science: Traversing Scale In Complex Many-Body Systems.

ACS Cent Sci 2021 Aug 28;7(8):1271-1287. Epub 2021 Jul 28.

Computer Science and Engineering Department, University of Connecticut, Storrs, Connecticut 06269-4155, United States.

A roadmap is developed that integrates simulation methodology and data science methods to target new theories that traverse the multiple length- and time-scale features of many-body phenomena.
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http://dx.doi.org/10.1021/acscentsci.1c00685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393217PMC
August 2021

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J Pharmacol Exp Ther 2021 Aug 31. Epub 2021 Aug 31.

The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China

Acute respiratory distress syndrome (ARDS), a common and fatal clinical condition, is characterized by the destruction of epithelium and augmented permeability of the alveolar-capillary barrier. Resolvin conjugates in tissue regeneration (RCTR1) is an endogenous lipid mediator derived from DHA, exerting pro-resolution effects in the process of inflammation. In our research, we evaluated the role of RCTR1 in alveolar fluid clearance (AFC) in Lipopolysaccharide-induced ARDS/ALI rat model. Rats were injected with RCTR1 (5 μg/kg) via caudal veins 8h after LPS (14 mg/kg) treatment, then AFC was estimated after 1h of ventilation. Primary type II alveolar epithelial cells (AEC II) were incubated with LPS (1 ug/ml) with or without RCTR1 (10 nM) for 8 h. Our results showed that RCTR1 significantly enhanced the survival rate, promoted the AFC, and alleviated LPS-induced ARDS/ALI in vivo. Furthermore, RCTR1 remarkably elevated the protein expression of sodium channels and Na, K-ATPase, and the activity of Na, K-ATPase in vivo and in vitro. Additionally, RCTR1 also decreased Nedd4-2 level via up-regulating P-Akt expression. Besides, inhibitors of ALX, cAMP, and PI3K (BOC-2, KH-7, and LY294002) notably inhibited the effects of RCTR1 on AFC. In summary, RCTR1 enhances the protein levels of sodium channels and Na, K-ATPase, and the Na, K-ATPase activity to improve AFC in ALI through ALX/cAMP/PI3K/Nedd4-2 pathway, suggesting that RCTR1 may become a therapeutic drug for ARDS/ALI. 1.RCTR1, an endogenous lipid mediator, enhanced the rate of AFC to accelerate the resolution of inflammation in the LPS-induced murine lung injury model. 2. RCTR1 up-regulates the expression of ENaC and Na, K-ATPase in vivo and in vitro to accelerate the AFC. 3. The efficacy of RCTR1 on the ENaC and Na, K-ATPase level was in an ALX/cAMP/PI3K/Nedd4-2-dependent.
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http://dx.doi.org/10.1124/jpet.121.000712DOI Listing
August 2021
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