Publications by authors named "Qian Wu"

1,048 Publications

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Transcriptomic encoding of sensorimotor transformation in the midbrain.

Elife 2021 Jul 28;10. Epub 2021 Jul 28.

Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Sensorimotor transformation, a process that converts sensory stimuli into motor actions, is critical for the brain to initiate behaviors. Although the circuitry involved in sensorimotor transformation has been well delineated, the molecular logic behind this process remains poorly understood. Here, we performed high-throughput and circuit-specific single-cell transcriptomic analyses of neurons in the superior colliculus (SC), a midbrain structure implicated in early sensorimotor transformation. We found that SC neurons in distinct laminae express discrete marker genes. Of particular interest, and are key markers that define glutamatergic projection neurons in the optic nerve (Op) and intermediate gray (InG) layers, respectively. The Cbln2+ neurons responded to visual stimuli mimicking cruising predators, while the Pitx2+ neurons encoded prey-derived vibrissal tactile cues. By forming distinct input and output connections with other brain areas, these neuronal subtypes independently mediate behaviors of predator avoidance and prey capture. Our results reveal that, in the midbrain, sensorimotor transformation for different behaviors may be performed by separate circuit modules that are molecularly defined by distinct transcriptomic codes.
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http://dx.doi.org/10.7554/eLife.69825DOI Listing
July 2021

Catechin Inhibits the Release of Advanced Glycation End Products during Glycated Bovine Serum Albumin Digestion and Corresponding Mechanisms .

J Agric Food Chem 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Chang jiang West Road, Hefei, Anhui 230036, China.

Glycated proteins are the main source of dietary advanced glycation end products (AGEs). Glycated proteins are enzymatically hydrolyzed in the gastrointestinal tract, which releases more absorbable and smaller potentially harmful AGEs. This study investigated the inhibitory effect of catechin on AGE release from glycated bovine serum albumin (G-BSA) during gastrointestinal digestion. Catechin inhibited AGE release during gastrointestinal digestion, especially in the gastric digestion stage. Additionally, catechin altered these peptides in the small intestine by reducing G-BSA digestibility. The proposed mechanism involves interactions between catechin and G-BSA/digestive enzymes, inhibiting digestive enzyme activity and changing the conformation of G-BSA. Catechin reduced G-BSA β-sheet content and protected the helical conformation. Moreover, catechin enhanced the antioxidant capacity of G-BSA, which could attenuate postprandial oxidative stress in the gastrointestinal tract caused by the release of AGEs. This study improves our understanding of the nutritional and health effects of catechin on dietary AGEs during gastrointestinal digestion.
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http://dx.doi.org/10.1021/acs.jafc.1c03348DOI Listing
July 2021

Study on the bioaccessibility and bioavailability of Cd in contaminated rice in vitro and in vivo.

J Food Sci 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of Education, Wuhan Polytechnic University, Wuhan, Hubei, China.

Cadmium (Cd) is a widespread heavy metal pollutant in the environment that damages human health. In this study, the bioaccessibility and bioavailability of Cd in different Cd-contaminated rice (low pollution level cadmium rice (Rice-L, 0.111 mg/kg), medium pollution level cadmium rice (Rice-M, 0.400 mg/kg), and high pollution level cadmium rice (Rice-H, 0.655 mg/kg)) were estimated and determined by an in vitro digestion model Rijksinstituut voor volksgezondheiden milieu (RIVM), Caco-2 cell model, and mouse model. The results indicated that Cd in the oral cavity (15.65-28.28%) displayed the lowest bioaccessibility comparing with small intestine (90.04-94.73%) and the stomach (99.30-100.70%) in vitro after cooking. In addition, the results showed that the bioaccessibility of Cd in CdCl , CdCl +normal rice (Rice-N), Rice-H, Rice-M, Rice-L group were 99.29%, 92.57%, 90.04%, 94.73%, and 91.11%, respectively; the in vitro bioavailability of Cd in CdCl , CdCl +Rice-N, Rice-H, Rice-M, and Rice-L group were 27.50%, 20.78%, 21.90%, 26.90%, 36.46%, respectively, we found that the group of CdCl is significantly higher than CdCl +Rice-N and Rice-H (p < 0.05), while the targets hazard quotient (THQ) value of rice ingested without considering the in vitro bioavailability is 2.7-4.6 times than the THQ value with considered and the relative bioavailability (RBA) of Cd in Rice-L, Rice-M, Rice-H are 80.25%, 64.32%, and 60.91%, respectively. These results indicate that the rice substrate has impact on the bioaccessibility and bioavailability of Cd, and might overestimate the health risks of Cd if bioavailability was not considered. PRACTICAL APPLICATION: Studying the bioaccessibility and bioavailability of cadmium in rice is a promising strategy to obtain a more accurate human health risk assessment of cadmium exposure in rice, as well as provide a theoretical basis for the formulation of cadmium limit standard in grain, which was also conducive to the rational and full utilization of rice resources in China.
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http://dx.doi.org/10.1111/1750-3841.15829DOI Listing
July 2021

Sulforaphane protects intestinal epithelial cells against lipopolysaccharide-induced injury by activating the AMPK/SIRT1/PGC-1ɑ pathway.

Bioengineered 2021 Dec;12(1):4349-4360

Department of Pharmacy, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, China.

The naturally occurring isothiocyanate sulforaphane, found in vegetables, shows promising anti-inflammatory, anti-apoptosis, and anti-oxidative effects. Whether sulforaphane protects against lipopolysaccharide (LPS)-induced injury in intestinal epithelial cells is unclear. The present study examines the ability of sulforaphane to protect Caco-2 cultures from LPS-induced injury, as well as the mechanism involved. Caco-2 cells were incubated for 24 h with 1 μg/mL LPS and different concentrations of sulforaphane (0.1-10 μM). Then, various indicators of oxidative stress, inflammation, apoptosis, and intestinal permeability were assayed. Sulforaphane increased cell viability and reduced lactate dehydrogenase activity in LPS-treated Caco-2 cells in a concentration-dependent manner. Sulforaphane weakened LPS-induced increases in intestinal epithelial cell permeability and oxidative stress (based on assays of reactive oxygen species, DMA, and HO), and it increased levels of antioxidants (SOD, GPx, CAT and T-AOC). At the same time, sulforaphane weakened the ability of LPS to induce production of inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) and the pro-apoptotic caspases-3 and -9. Sulforaphane also upregulated p-AMPK, SIRT1, and PGC-1ɑ, whose inhibitors antagonized the compound's protective effects. Sulforaphane can protect intestinal epithelial cells against LPS-induced changes in intestinal permeability, oxidative stress, inflammation, and apoptosis. It appears to act by activating the AMPK/SIRT1/PGC-1ɑ pathway. The drug therefore shows potential for preventing LPS-induced intestinal injury.
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http://dx.doi.org/10.1080/21655979.2021.1952368DOI Listing
December 2021

The Association Between Polymorphisms and Treatment Response in Patients With Epilepsy.

Front Pharmacol 2021 9;12:701575. Epub 2021 Jul 9.

Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Epilepsy is a debilitating brain disease with complex inheritance and frequent treatment resistance. However, the role of single nucleotide polymorphisms (SNPs) in epilepsy treatment remains unknown. This study aimed to explore the genetic association of SNPs with treatment response in patients with epilepsy in a Han Chinese population. We first examined the associations between SNPs and epilepsy in 1000 Han Chinese and the associations between SNPs and drug-resistant epilepsy in 450 subjects. Expression quantitative trait loci analysis was then conducted using 16 drug-resistant epileptic brain tissue samples and results from the BrainCloud database (http://eqtl.brainseq.org). The allelic frequencies of rs140820592 were different between the epilepsy and control groups ( = 0.002) after Bonferroni correction. The rs140820592 was associated with significantly lower epilepsy risk among 1,000 subjects in the dominant model after adjusting for gender and age and Bonferroni correction (OR = 0.542, 95%CI = 0.358-0.819, = 0.004). The rs140820592 also conferred significantly lower risk of drug-resistant epilepsy among 450 subjects using the same dominant model after adjusting for gender and age and Bonferroni correction (OR = 0.260, 95%CI = 0.103-0.653, = 0.004). Expression quantitative trait loci analysis revealed that rs140820592 was associated with expression level in drug-resistant epileptic brain tissues ( = 0.012), and this result was further verified in the BrainCloud database (http://eqtl.brainseq.org) ( = 2.3214 × 10). The rs140820592 may influence the risks of epilepsy and drug-resistant epilepsy by regulating expression in brain tissues.
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http://dx.doi.org/10.3389/fphar.2021.701575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299048PMC
July 2021

Depressive symptoms and 5-year incident metabolic syndrome among older adults.

Sci Rep 2021 Jul 21;11(1):14842. Epub 2021 Jul 21.

School of Public Health, Medical College of Soochow University, 199 Ren Ai Road, Suzhou, 215123, China.

Little is known regarding the association between depressive symptoms and metabolic syndrome (MetS) among older Chinese adults. This study aimed to examine the association of depressive symptoms with MetS and its components among Chinese elderly. Based on whether they showed depressive symptoms at baseline, 262 age-gender-matched participants from a community-based cohort study were included. The presence of depressive symptoms was measured using the nine-item Patient Health Questionnaire (PHQ-9). MetS was defined according to the Adult Treatment Panel III of the National Cholesterol Education Program. Linear regression and logistic regression analyses were performed to assess associations of depressive symptoms with MetS and its components. The incidence of MetS among the participants with depressive symptoms at baseline was 15.27% (20/131). The association of the presence of depressive symptoms with MetS was significant (odds ratio [OR] = 2.53, 95% confidence intervals [CI] = 1.07, 5.95). There was a negative association between depressive symptoms and hypertension (OR = 0.04, 95% CI = 0.002, 0.98). The change in mean arterial pressure varies approximately 1.03 mmHg with a 1-point change in PHQ-9 score. In this study, baseline depressive symptoms were associated with subsequent MetS. The presence of depressive symptoms was negatively associated with elevated mean arterial pressure.
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http://dx.doi.org/10.1038/s41598-021-94503-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295297PMC
July 2021

Usefulness of readout-segmented EPI-based diffusion tensor imaging of lacrimal gland for detection and disease staging in thyroid-associated ophthalmopathy.

BMC Ophthalmol 2021 Jul 20;21(1):281. Epub 2021 Jul 20.

Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Address: No. 300, Guangzhou Rd, Gulou district, Nanjing, P.R. China.

Background: Dysfunction of lacrimal gland (LG) gains increasing attention in patients with thyroid-associated ophthalmopathy (TAO), while the underlying pathological change is still not fully established. This study aimed to evaluate the utility of readout-segmented echo-planar imaging (rs-EPI)-based diffusion tensor imaging (DTI) in non-invasively detecting microstructural alterations of LG in patients with TAO, as well as in discriminating disease activity.

Methods: Thirty TAO patients and 15 age- and sex- matched healthy controls, who underwent rs-EPI-based DTI, were retrospectively enrolled. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) of LG, and clinical-endocrinological variables were collected and compared. The correlations between FA and ADC values of LG and serum thyroid biochemical markers were also assessed.

Results: TAO group showed significantly lower FA (P < 0.001) and higher ADC (P = 0.014) of LG than healthy group. Active subgroup had significantly lower FA (P < 0.001) and higher ADC (P < 0.001) than inactive subgroup. In TAO group, FA of LG was significantly and negatively correlated with TRAb (r=-0.475, P = 0.008), while ADC of LG showed no significant correlation (P > 0.05). The area under receiver operating characteristic curve of FA was significantly greater than that under curve of ADC for discriminating disease activity (0.832 vs. 0.570, P = 0.009).

Conclusions: rs-EPI-based DTI is a useful tool to characterize the microstructural change of LG in patients with TAO. The derived metrics, particularly FA, can help to reveal disease activity.
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http://dx.doi.org/10.1186/s12886-021-02044-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290601PMC
July 2021

Association between autoimmune encephalitis and epilepsy: Systematic review and meta-analysis.

Seizure 2021 Jul 12;91:346-359. Epub 2021 Jul 12.

Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, PR China. Electronic address:

Background: Diverse neuronal antibodies are related to autoimmune encephalitis (AE) and AE-related epilepsy. However, the epidemiological characteristics of AE, AE-associated antibodies, and AE-related seizures are still unclear.

Aims: This research evaluated the relationship between AE, AE-related seizures, and neuronal antibodies, as well as the morbidity of AE with early incidence.

Methods: The PubMed, Embase, Cochrane, and Web of Science databases were searched. Pooled estimates and 95% confidence intervals (CIs) were calculated using a random-effects model.

Results: Of the 4,869 citations identified, 100 articles were reviewed in full, and 42 subgroups were analyzed. The overall incidence of AE patients with seizures was 42% (95% CI: 0.40-0.44), and among them, the incidence of epilepsy in anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis patients was 73% (95% CI: 0.70-0.77). Subsequently, we found that the prevalence of AE as the cause of epilepsy within the pooled period was 1% (95% CI: 0.01-0.02), while the overall positive rate of neuronal antibodies in epilepsy patients was 4% (95% CI: 0.03-0.05). Additionally, the detection rates of different antibodies among epilepsy patients were as follows: anti-NMDAR, 1%; anti-leucine-rich glioma inactivated 1 (LGI1), 1%; anti-contactin-associated protein-like 2 (CASPR2), 2%.

Conclusion: Based on our findings, neuronal antibodies may serve as a bridge to study AE and immune-related epilepsy. To further understand the differences in outcomes following different treatment measures, and to provide more information for public health policy and prevention, more research is needed to improve the accuracy of estimations.
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http://dx.doi.org/10.1016/j.seizure.2021.07.005DOI Listing
July 2021

Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.

PLoS One 2021 15;16(7):e0254711. Epub 2021 Jul 15.

College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, China.

GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species' GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254711PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282010PMC
July 2021

Data storage using peptide sequences.

Nat Commun 2021 07 13;12(1):4242. Epub 2021 Jul 13.

State Key Laboratory of Chemical Biology and Drug Discovery, Research Institute for Future Food and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, China.

Humankind is generating digital data at an exponential rate. These data are typically stored using electronic, magnetic or optical devices, which require large physical spaces and cannot last for a very long time. Here we report the use of peptide sequences for data storage, which can be durable and of high storage density. With the selection of suitable constitutive amino acids, designs of address codes and error-correction schemes to protect the order and integrity of the stored data, optimization of the analytical protocol and development of a software to effectively recover peptide sequences from the tandem mass spectra, we demonstrated the feasibility of this method by successfully storing and retrieving a text file and the music file Silent Night with 40 and 511 18-mer peptides respectively. This method for the first time links data storage with the peptide synthesis industry and proteomics techniques, and is expected to stimulate the development of relevant fields.
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http://dx.doi.org/10.1038/s41467-021-24496-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277807PMC
July 2021

A single luminescence center ultra-broadband near-infrared LiScGeO:Cr phosphor for biological tissue penetration.

Dalton Trans 2021 Jul;50(29):10092-10101

National-Local Joint Engineering Laboratory of New Energy Photoelectric Devices, Hebei Key Laboratory of Optic-electronic Information and Materials, College of Physics Science & Technology, Hebei University, Baoding 071002, China.

In this work, in order to meet the application of near-infrared phosphor-converted light emitting diodes (pc-LEDs), an ultra-broadband emission phosphor, LiScGeO4:Cr, was synthesized. Its FWHM reaches 335 nm, and its emission spectrum ranges from 800 nm to 1650 nm, which almost covers the entire near-infrared second window (NIR-II). The broadband emission is thought to be caused by the 4T2 → 4A2 transition of the Cr3+ ion. This transition occurs due to the olivine structure of the crystal, which causes the Cr3+ ions to inhabit a low-symmetric crystal field, and the crystal field strength is very weak. NIR pc-LEDs were fabricated by combining a 460 nm blue LED with this phosphor, which penetrates 4 cm thick beef. The results indicate that there may be a potential application for this phosphor in the field of biological tissue penetration and non-destructive testing.
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http://dx.doi.org/10.1039/d1dt01244dDOI Listing
July 2021

Non-causal effects of smoking and alcohol use on the risk of systemic lupus erythematosus.

Autoimmun Rev 2021 Jul 6;20(9):102890. Epub 2021 Jul 6.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China; Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, 81 Meishan Road, Hefei 230032, Anhui, China. Electronic address:

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http://dx.doi.org/10.1016/j.autrev.2021.102890DOI Listing
July 2021

Interleukin-10 level is associated with post-stroke depression in acute ischaemic stroke patients.

J Affect Disord 2021 Jun 25;293:254-260. Epub 2021 Jun 25.

Department of Rehabilitation, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Background: Interleukin-10 (IL-10) is a pathophysiological factor in acute ischaemic stroke (AIS) and is relevant to mood disorders after stroke. We evaluated the predictive value of IL-10 in patients with post-stroke depression (PSD).

Methods: A total of 350 stroke patients were recruited at baseline, and 151 AIS patients were screened and completed a 1-month follow-up. Serum IL-10 levels were measured within 24 h of admission. We used the 17-item Hamilton Depression Scale (HAMD-17) to evaluate depression symptoms; PSD was defined as an HAMD score ≥ 7.

Results: Fifty-one (33.8%) patients showed a more serious stroke degree, larger infarction volume, and poorer daily life activities and prognosis (P < 0.05) and were diagnosed with PSD at the 1-month follow-up. Their IL-10 level decreased significantly compared to the non-PSD group (P < 0.001). After adjusting for confounders, IL-10 could be used as an independent predictor for PSD with an adjusted odds ratio (OR) of 0.615 (95% CI 0.410-0.923, P = 0.019). In addition, the optimal cut-off value of IL-10 was 0.615 pg/mL based on an area under the receiver operating characteristic curve of 0.692 (95% CI 0.604-0.781, P < 0.001), demonstrating that IL-10 could predict the occurrence of PSD. Moreover, IL-10 was an indicator of stroke severity, living ability, and functional outcomes (P < 0.05).

Limitations: IL-10 was only measured upon admission; dynamic changes need to be further monitored. This was also a single-centre study with a relatively small sample.

Conclusions: Lower IL-10 levels may be used to predict PSD.
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http://dx.doi.org/10.1016/j.jad.2021.06.037DOI Listing
June 2021

Maternal genetic structure of a neolithic population of the Yangshao culture.

J Genet Genomics 2021 May 19. Epub 2021 May 19.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, Beijing 100044, China; University of Chinese Academy of Sciences 100049, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.04.005DOI Listing
May 2021

Sustained calcium ion release from bioceramics promotes CaSR-mediated M2 macrophage polarization for osteoinduction.

J Leukoc Biol 2021 Jun 28. Epub 2021 Jun 28.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Innate immune cells, especially macrophages, play a dual role in tissue repair and the defense against foreign bodies. Although biphasic calcium phosphate (BCP) ceramics have been confirmed as an excellent osteoimmunoregulatory biomaterial, it is unclear whether the ions release of BCP directly affects macrophage polarization and the mechanism by which the ions release is involved in osteoimmunomodulation. Herein, we verified the superior osteoinductive capacity of BCP in wild-type mice and showed its inability to promote this process in macrophage-deficient (LysM ) mice. Moreover, scanning electron microscopy, ion release curve, and calcein AM-staining results confirmed that BCP-released Ca in a sustained manner, thereby maintaining the long-term induction of M2 macrophage polarization and promoting the differentiation of mesenchymal stem cells into osteoblasts during osteogenesis. Furthermore, Ca targeted the Wnt/β-catenin signaling pathway and activated Arg1 and IL-10 (M2 marker genes) transcription through the calcium-sensing receptor (CaSR) in macrophages. Under treatment with a CaSR antagonist, macrophages cultured with the BCP fluid extract exhibited lower Ca intake and weaker M2 macrophage polarization. These findings underscore the critical role of macrophages in bone regeneration and clarify the molecular mechanisms of Ca -mediated osteoinduction by biomaterials, which is of great significance for the future design of biomaterial-oriented tissue regeneration engineering.
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http://dx.doi.org/10.1002/JLB.3MA0321-739RDOI Listing
June 2021

Interference of Illusory Contour Perception by a Distractor.

Front Psychol 2021 11;12:526972. Epub 2021 Jun 11.

Department of Psychology, Sun Yat-sen University, Guangzhou, China.

The visual system is capable of recognizing objects when object information is widely separated in space, as revealed by the Kanizsa-type illusory contours (ICs). Attentional involvement in perception of ICs is an important topic, and the present study examined whether and how the processing of ICs is interfered with by a distractor. Discrimination between thin and short deformations of an illusory circle was investigated in the absence or presence of a central dynamic patch, with difficulty of discrimination varied in three levels (easy, medium, and hard). Reaction time (RT) was significantly shorter in the absence compared to the presence of the distractor in the easy and medium conditions. Correct rate (CR) was significantly higher in the absence compared to the presence of the distractor in the easy condition, and the magnitude of the difference between CRs of distracted and non-distracted responses significantly reduced as task difficulty increased. These results suggested that perception of ICs is more likely to be vulnerable to distraction when more attentional resources remain available. The present finding supports that attention is engaged in perception of ICs and that distraction of IC processing is associated with perceptual load.
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http://dx.doi.org/10.3389/fpsyg.2021.526972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231925PMC
June 2021

Disrupted Spontaneous Neural Activity in Patients With Thyroid-Associated Ophthalmopathy: A Resting-State fMRI Study Using Amplitude of Low-Frequency Fluctuation.

Front Hum Neurosci 2021 11;15:676967. Epub 2021 Jun 11.

Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: The purpose of the study was to investigate the brain functional alteration in patients with thyroid-associated ophthalmopathy (TAO) by evaluating the spontaneous neural activity changes using resting-state functional magnetic resonance imaging (rs-fMRI) with the amplitude of low-frequency fluctuation (ALFF) method.

Materials And Methods: The rs-fMRI data of 30 TAO patients (15 active and 15 inactive) and 15 healthy controls (HCs) were included for analyses. The ALFF values were calculated and compared among groups. Correlations between ALFF values and clinical metrics were assessed.

Results: Compared with HCs, active TAOs showed significantly decreased ALFF values in the left middle occipital gyrus, superior occipital gyrus, and cuneus. Compared with inactive TAOs, active TAOs showed significantly increased ALFF values in the bilateral precuneus. Additionally, inactive TAOs showed significantly decreased ALFF values in the left middle occipital gyrus, superior occipital gyrus, cuneus, and bilateral precuneus than HCs. The ALFF value in the right precuneus of TAOs was positively correlated with clinical activity score ( = 0.583, < 0.001) and Mini-Mental State Examination (MMSE) score ( = 0.377, = 0.040), and negatively correlated with disease duration ( = -0.382, = 0.037). Moreover, the ALFF value in the left middle occipital gyrus of TAOs was positively correlated with visual acuity ( = 0.441, = 0.015).

Conclusion: TAO patients had altered spontaneous brain activities in the left occipital lobe and bilateral precuneus. The neuropsychological aspect of the disease should be noticed during clinical diagnosis and treatment.
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http://dx.doi.org/10.3389/fnhum.2021.676967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226248PMC
June 2021

Reactive oxygen FIB spin milling enables correlative workflow for 3D super-resolution light microscopy and serial FIB/SEM of cultured cells.

Sci Rep 2021 Jun 23;11(1):13162. Epub 2021 Jun 23.

Thermo Fisher Scientific, 5350 NE Dawson Creek Drive, Hillsboro, OR, 97124, USA.

Correlative light and electron microscopy (CLEM) is a powerful tool for defining the ultrastructural context of molecularly-labeled biological specimens, particularly when superresolution fluorescence microscopy (SRM) is used for CLEM. Current CLEM, however, is limited by the stark differences in sample preparation requirements between the two modalities. For CLEM using SRM, the small region of interest (ROI) of either or both modalities also leads to low success rate and imaging throughput. To overcome these limitations, here we present a CLEM workflow based on a novel focused ion beam/scanning electron microscope (FIB/SEM) compatible with common SRM for imaging biological specimen with ultrahigh 3D resolution and improved imaging throughput. By using a reactive oxygen source in a plasma FIB (PFIB) and a rotating sample stage, the novel FIB/SEM was able to achieve several hundreds of micrometer large area 3D analysis of resin embedded cells through a process named oxygen serial spin mill (OSSM). Compared with current FIB mechanisms, OSSM offers gentle erosion, highly consistent slice thickness, reduced charging during SEM imaging, and improved SEM contrast without increasing the dose of post-staining and fixation. These characteristics of OSSM-SEM allowed us to pair it with interferometric photoactivated localization microscopy (iPALM), a recent SRM technique that affords 10-20 nm isotropic spatial resolution on hydrated samples, for 3D CLEM imaging. We demonstrate a CLEM workflow generalizable to using other SRM strategies using mitochondria in human osteosarcoma (U2OS) cells as a model system, where immunostained TOM20, a marker for the mitochondrial outer membrane, was used for iPALM. Owing to the large scan area of OSSM-SEM, it is now possible to select as many FOVs as needed for iPALM and conveniently re-locate them in EM, this improving the imaging throughput. The significantly reduced dose of post-fixation also helped to better preserve the sample ultrastructures as evidenced by the excellent 3D registration between OSSM-SEM and iPALM images and by the accurate localization of TOM20 (by iPALM) to the peripheries of mitochondria (by OSSM-SEM). These advantages make OSSM-SEM an ideal modality for CLEM applications. As OSSM-SEM is still in development, we also discuss some of the remaining issues and the implications to biological imaging with SEM alone or with CLEM.
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http://dx.doi.org/10.1038/s41598-021-92608-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222390PMC
June 2021

Reduced emergency room visits and improved medication adherence of an integrated oncology pharmaceutical care practice in China.

J Oncol Pharm Pract 2021 Jun 24:10781552211027218. Epub 2021 Jun 24.

Department of Pharmacy, Huashan Hospital, Fudan University, Shanghai, China.

Objective: We described our initial experience of a new integrated oncology phamaceutical care practice to enhance the quality of pharmacy service and patient care in Huashan hospital. A retrospective study was performed from August 2019 to September 2020. Patients were described as integrated pharmacy service group and routine care group. Medication adherence of patients in integrated pharmacy service group was recorded by the online management system. Patient satisfaction and the cumulative incidence of emergency room (ER) and outpatient visit were evaluated between two groups. In total, 323 patients received the integrating oncology pharmacy service. The percentage of the patients missing administration every day was reduced from 29.7% to 0.3% within a 40-day monitoring and intervention period. There was a significant difference on patient satisfaction with pharmacy service in two groups ( 0.05). Fewer patients in the integrated pharmacy service group visited clinic and ER compared with routine care group (33.1% 59.2%; 0.05).

Conclusions: As a new practice model, the integrated program is adopted to provide patient care and ongoing monitoring for cancer patients. The practice model delivers high continuity of care for cancer patients and improves communication and collaboration between healthcare professionals and oncology patients. The practice also provides the potential of developing hospital pharmaceutical service and optimizing disease prevention and treatment strategies.
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http://dx.doi.org/10.1177/10781552211027218DOI Listing
June 2021

Features of CT and EUS in mesenteric plexiform neurofibroma with Neurofibromatosis type I: A case report.

Radiol Case Rep 2021 Aug 9;16(8):2103-2107. Epub 2021 Jun 9.

Department of Radiology, the Second Affiliated Hospital of Soochow University, Suzhou, China.

Plexiform neurofibroma(PNF) is a rare benign tumor of the peripheral nerve, belonging to a subtype of neurofibroma. PNF is common in the head, neck and trunk. It is uncommonly observed in the mesentery. We report a case of mesenteric PNF in a 64-year-old man history of neurofibromatosis type I(NF1), which caused abdomen pain. In addition, the computer tomography(CT) and endoscopic ultrasonography(EUS) manifestations of mesenteric PNF were analyzed. The imaging appearance of a mesenteric plexiform neurofibroma is that many low-density (CT) /mixed echo (EUS) soft tissue masses surrounding the superior mesenteric artery, but not surrounding the superior mesenteric vein. Our case adds to the limited literature regarding NF1 presenting with mesenteric PNF. The computer tomography and endoscopic ultrasonography may facilitate confirma diagnosis of mesenteric PNF.
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http://dx.doi.org/10.1016/j.radcr.2021.04.071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203574PMC
August 2021

A Phase II Study Evaluating the Safety and Efficacy of Sunitinib Malate in Combination With Weekly Paclitaxel Followed by Doxorubicin and Daily Oral Cyclophosphamide Plus G-CSF as Neoadjuvant Chemotherapy for Locally Advanced or Inflammatory Breast Cancer.

Clin Breast Cancer 2021 May 24. Epub 2021 May 24.

Medical Oncology, University of Washington, Seattle, WA; Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA. Electronic address:

Introduction: Neoadjuvant chemotherapy is standard treatment for locally advanced breast cancer (LABC) or inflammatory breast cancer (IBC). We hypothesized that adding sunitinib, a tyrosine kinase inhibitor with antitumor and antiangiogenic activity, to an anthracycline and taxane regimen would improve pathologic complete response (pCR) rates to a prespecified endpoint of 45% in patients with HER2-negative LABC or IBC.

Methods: We conducted a multicenter, phase II trial of neoadjuvant sunitinib with paclitaxel (S+T) followed by doxorubicin and cyclophosphamide plus G-CSF for patients with HER2-negative LABC or IBC. Patients received sunitinib 25 mg PO daily with paclitaxel 80 mg/m IV weekly ×12 followed by doxorubicin 24 mg/m IV weekly + cyclophosphamide 60 mg/m PO daily with G-CSF support. Response was evaluated using pCR in the breast and the CPS + EG score (clinical-pathologic scoring + estrogen receptor [ER] and grade).

Results: Seventy patients enrolled, and 66 were evaluable for efficacy. Eighteen patients (27%) had pCR in the breast (10 had ER disease and 8 had triple-negative disease). When defining response as pCR and/or CPS + EG score ≤2, 31 (47%) were responders. In pateints with ER positive disease, 23 (64%) were responders. The most common toxicities were cytopenias and fatigue.

Conclusions: Neoadjuvant S+T followed by AC+G-CSF was safe and tolerable in LABC and IBC. The study did not meet the prespecified endpoint for pCR; however, 47% were responders using pCR and/or CPS + EG score ≤2. ER positive patients had the highest response rate (64%). The addition of sunitinib to neoadjuvant chemotherapy may provide promising incremental benefit for patients with ER positive LABC.
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http://dx.doi.org/10.1016/j.clbc.2021.05.009DOI Listing
May 2021

Characterization and phylogenetic analysis of the complete chloroplast genome sequence of (Asteraceae), a rare and endangered wild plant species in China.

Mitochondrial DNA B Resour 2021 Jun 7;6(7):1934-1936. Epub 2021 Jun 7.

College of Life Sciences, Taizhou University, Jiaojiang, China.

(Asteraceae) is a rare wild plant species narrowly distributed in Zhejiang province, China. In our present study, we assembled its complete chloroplast genome using high-throughput sequencing data. The results indicated that the whole chloroplast genome of was 153,396 bp in length. Its large single copy, small single copy, and inverted region sequences were 84,575 bp, 18,451 bp, and 25,185 bp. The chloroplast genome was composed of 134 genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes, and two pseudogenes. Phylogenetic analysis results showed that was grouped with , with 100% bootstrap support.
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http://dx.doi.org/10.1080/23802359.2021.1936671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189106PMC
June 2021

Indoor location technology based on LED visible light and QR code.

Authors:
Qian Wu Yanxiao He

Appl Opt 2021 Jun;60(16):4606-4612

For the current problems of extremely complex visible light indoor positioning algorithms and devices and low positioning accuracy, this paper proposes an improved indoor positioning method combining light-emitting diode (LED) visible light and quick response (QR) codes. First, initial positioning is achieved by loading a QR code image containing LED position information on the LED light, which is recognized and decoded by the receiver. Then the position of the receiver is precisely located by proposing an improved indoor positioning method. The experiment shows the smallest average error of 4.0326 cm in positioning, which greatly improves the indoor positioning accuracy.
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http://dx.doi.org/10.1364/AO.426233DOI Listing
June 2021

Comparison of the Wiltse Approach and Percutaneous Pedicle Screw Fixation Under O-arm Navigation for the Treatment of Thoracolumbar Fractures.

Orthop Surg 2021 Jul 17;13(5):1618-1627. Epub 2021 Jun 17.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objectives: The aim of this study was to evaluate the clinical outcomes of the Wiltse approach and percutaneous pedicle screw placement under O-arm navigation for the treatment of thoracolumbar fracture.

Methods: We enrolled a total of 54 patients with neurologically intact thoracolumbar fracture who received minimally invasive treatments between October 2014 and October 2018 in this retrospective study. Among these, 28 patients (22 males and six females, with a mean age of 48.6 ± 9.6 years) were treated with pedicle screw fixation through the Wiltse approach (WPSF), and another 26 (15 males and 11 females, with a mean age of 45.7 ± 10.6 years) received percutaneous pedicle screw fixation under O-arm navigation (OPSF). Statistical methods were used to perform a detailed comparison of clinical outcomes, radiologic findings, and complications between the two groups obtained preoperatively, postoperatively, and at last follow-up.

Results: All patients underwent surgery successfully and finished a follow-up of more than 12 months. No serious complications, such as infection, blood vessel injury, or spinal cord or nerve root injury occurred. Visual analog scale (VAS) scores, Oswestry disability index (ODI) scores, local Cobb angle (LCA), vertebral wedge angle (VWA), and R value were notably improved after surgery, though there was no clear discrepancy between the groups at each time point (P > 0.05). During the follow-up period, no patients developed neurological impairment or implant-related complications, and no patients underwent revision surgery. The WPSF group had a significantly shorter operation time than the OPSF group (68.1 ± 9.8 vs 76.1 ± 9.0 minutes, P = 0.005). Moreover, the WPSF group showed less cost of surgery than the WPSF group (48142.1 ± 1430.1 vs 59035.4 ± 1152.7 CNY, P < 0.001). There were no significant differences between the two groups in terms of the intraoperative bleeding, length of incision, or postoperative hospitalization time (P > 0.05). The accuracy of pedicle screw placement was 95.2% (160/168) in the WPSF group and 96.8% (151/156) in the OPSF group, with no significant difference between the groups (P = 0.432).

Conclusion: Both WPSF and OPSF were safe and effective for the treatment of thoracolumbar fracture. Although the two groups showed favorable clinical and radiologic outcomes through to final follow-up, we recommended the minimally invasive WPSF given its shorter operation time and lower cost of surgery.
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http://dx.doi.org/10.1111/os.13053DOI Listing
July 2021

Study on toxicological effect and the mechanism of cadmium in rice and inorganic cadmium on ICR mice.

Toxicol Res (Camb) 2021 May 1;10(3):639-650. Epub 2021 Jun 1.

Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of Education, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan 430023, Hubei, China.

Cadmium (Cd) exposure may induce chronic intoxication, but the harm of cadmium in rice to human at chronic low-level Cd exposure remains unclear. This study employed a mouse model to investigate the toxicity and mechanism of cadmium in rice and CdCl. After 8-week exposure to Cd (CdCl and Cd-contaminated rice), the biochemical indicators and oxidation indicators in the serum and liver of mice were determined, and used mRNA sequencing to investigate the mechanism of different forms of Cd. Results showed that the cadmium concentration of the liver in the CdCl + Rice-N group (CdCl mixed with feed and normal rice, 0.4mg/kg.bw) was higher than that in the Rice-H group (0.4mg/kg.bw). However, the cadmium concentration of the kidneys in the Rice-H group was higher than that in the CdCl + Rice-N group. Our study demonstrated that Cd-treated (Cd in rice and CdCl) ICR mice generated obviously tissues injury, such as the increased biochemical studies, the activity of antioxidant enzymes debasement. Simultaneously, our data also indicated that there existed difference of the hepatic toxicity between Cd in rice and CdCl. By means of transcriptomics, we discovered that CdCl and Cd in rice may affect different gene expression at the molecular level. We hope to provide some theoretical basis for the revision of food security standards.
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http://dx.doi.org/10.1093/toxres/tfab043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201557PMC
May 2021

The Sw-5b NLR nucleotide-binding domain plays a role in oligomerization and its self-association is important for the activation of cell death signaling.

J Exp Bot 2021 Jun 11. Epub 2021 Jun 11.

Key Laboratory of Plant Immunity, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.

Plant and animal intracellular nucleotide-binding and leucine-rich repeat (NLR) receptors play important roles in sensing pathogens and activating defense signaling. However, the molecular mechanisms underlying the activation of host defense signaling by NLR proteins remain largely unknown. Many previous studies have determined that the coil-coil (CC) or Toll and interleukin-1 receptor/resistance protein (TIR) domain of NLR proteins and their dimerization/oligomerization are critical for activating downstream defense signaling. In this study, we demonstrated that the nucleotide-binding (NB) domain of the tomato Sw-5b NLR alone can activate downstream defense signaling, leading to elicitor-independent cell death. Sw-5b NB domains can self-associate, and this self-association is crucial for activating cell death signaling. The self-association was strongly compromised after the introduction of a K568R mutation into the P-loop of the NB domain. Consequently, the NB K568R mutant induced cell death very weakly. The NB CΔ20 mutant lacking the C-terminal 20 amino acids can self-associate but cannot activate cell death signaling. The NB CΔ20 mutant also interfered with wild-type NB domain self-association, leading to compromised cell death induction. By contrast, the NB K568R mutant did not interfere with wild-type NB domain self-association and its ability to induce cell death. Structural modeling of Sw-5b suggests that NB domains associate with one another and likely participate in oligomerization. As Sw-5b-triggered cell death is dependent on helper NLR proteins, we propose that the Sw-5b NB domain acts as a nucleation point for the assembly of an oligomeric resistosome, probably by recruiting downstream helper partners, to trigger defense signaling.
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http://dx.doi.org/10.1093/jxb/erab279DOI Listing
June 2021

Novel Long Non-coding RNA and LASSO Prediction Model to Better Identify Pulmonary Tuberculosis: A Case-Control Study in China.

Front Mol Biosci 2021 25;8:632185. Epub 2021 May 25.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Introduction: The insufficient understanding and misdiagnosis of clinically diagnosed pulmonary tuberculosis (PTB) without an aetiological evidence is a major problem in the diagnosis of tuberculosis (TB). This study aims to confirm the value of Long non-coding RNA (lncRNA) n344917 in the diagnosis of PTB and construct a rapid, accurate, and universal prediction model.

Methods: A total of 536 patients were prospectively and consecutively recruited, including clinically diagnosed PTB, PTB with an aetiological evidence and non-TB disease controls, who were admitted to West China hospital from Dec 2014 to Dec 2017. The expression levels of lncRNA n344917 of all patients were analyzed using reverse transcriptase quantitative real-time PCR. Then, the laboratory findings, electronic health record (EHR) information and expression levels of n344917 were used to construct a prediction model through the Least Absolute Shrinkage and Selection Operator algorithm and multivariate logistic regression.

Results: The factors of n344917, age, CT calcification, cough, TBIGRA, low-grade fever and weight loss were included in the prediction model. It had good discrimination (area under the curve = 0.88, cutoff = 0.657, sensitivity = 88.98%, specificity = 86.43%, positive predictive value = 85.61%, and negative predictive value = 89.63%), consistency and clinical availability. It also showed a good replicability in the validation cohort. Finally, it was encapsulated as an open-source and free web-based application for clinical use and is available online at https://ziruinptb.shinyapps.io/shiny/.

Conclusion: Combining the novel potential molecular biomarker n344917, laboratory and EHR variables, this web-based prediction model could serve as a user-friendly, accurate platform to improve the clinical diagnosis of PTB.
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http://dx.doi.org/10.3389/fmolb.2021.632185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185277PMC
May 2021

High-resolution metabolomic biomarkers for lung cancer diagnosis and prognosis.

Sci Rep 2021 Jun 3;11(1):11805. Epub 2021 Jun 3.

Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming, 650118, Yunnan, China.

Lung cancer is the leading cause of human cancer mortality due to the lack of early diagnosis technology. The low-dose computed tomography scan (LDCT) is one of the main techniques to screen cancers. However, LDCT still has a risk of radiation exposure and it is not suitable for the general public. In this study, plasma metabolic profiles of lung cancer were performed using a comprehensive metabolomic method with different liquid chromatography methods coupled with a Q-Exactive high-resolution mass spectrometer. Metabolites with different polarities (amino acids, fatty acids, and acylcarnitines) can be detected and identified as differential metabolites of lung cancer in small volumes of plasma. Logistic regression models were further developed to identify cancer stages and types using those significant biomarkers. Using the Variable Importance in Projection (VIP) and the area under the curve (AUC) scores, we have successfully identified the top 5, 10, and 20 metabolites that can be used to differentiate lung cancer stages and types. The discrimination accuracy and AUC score can be as high as 0.829 and 0.869 using the five most significant metabolites. This study demonstrated that using 5 + metabolites (Palmitic acid, Heptadecanoic acid, 4-Oxoproline, Tridecanoic acid, Ornithine, and etc.) has the potential for early lung cancer screening. This finding is useful for transferring the diagnostic technology onto a point-of-care device for lung cancer diagnosis and prognosis.
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http://dx.doi.org/10.1038/s41598-021-91276-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175557PMC
June 2021

Texture analysis of orbital magnetic resonance imaging for monitoring and predicting treatment response to glucocorticoids in patients with thyroid-associated ophthalmopathy.

Endocr Connect 2021 Jun 24;10(7):676-684. Epub 2021 Jun 24.

Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To evaluate the value of MRI-based texture analysis of extraocular muscle (EOM) and orbital fat (OF) in monitoring and predicting the response to glucocorticoid (GC) therapy in patients with thyroid-associated ophthalmopathy (TAO).

Methods: Thirty-seven active and moderate-to-severe TAO patients (responders, n = 23; unresponders, n = 14) were retrospectively enrolled. MRI-based texture parameters (entropy, uniformity, skewness and kurtosis) of EOM and OF were measured before and after GC therapy, and compared between groups. Correlations between the changes of clinical activity score (CAS) and imaging parameters before and after treatment were assessed. Receiver operating characteristic curves were used to evaluate the predictive value of identified variables.

Results: Responsive TAOs showed significantly decreased entropy and increased uniformity at EOM and OF after GC therapy (P < 0.01), while unresponders showed no significance. Changes of entropy and uniformity at EOM and OF were significantly correlated with changes of CAS before and after treatment (P < 0.05). Responders showed significantly lower entropy and higher uniformity at EOM than unresponders before treatment (P < 0.01). Entropy and uniformity of EOM and disease duration were identified as independent predictors for responsive TAOs. Combination of all three variables demonstrated optimal efficiency (area under curve, 0.802) and sensitivity (82.6%), and disease duration alone demonstrated optimal specificity (100%) for predicting responsive TAOs.

Conclusion: MRI-based texture analysis can reflect histopathological heterogeneity of orbital tissues. It could be useful for monitoring and predicting the response to GC in TAO patients.
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http://dx.doi.org/10.1530/EC-21-0162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240707PMC
June 2021

Risk Score for Predicting Dysphagia in Patients After Neurosurgery: A Prospective Observational Trial.

Front Neurol 2021 11;12:605687. Epub 2021 May 11.

Department of Nursing, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Acquired dysphagia is common in patients with tracheal intubation and neurological disease, leading to increased mortality. This study aimed to ascertain the risk factors and develop a prediction model for acquired dysphagia in patients after neurosurgery. A multicenter prospective observational study was performed on 293 patients who underwent neurosurgery. A standardized swallowing assessment was performed bedside within 24 h of extubation, and logistic regression analysis with a best subset selection strategy was performed to select predictors. A nomogram model was then established and verified. The incidence of acquired dysphagia in our study was 23.2% (68/293). Among the variables, days of neurointensive care unit (NICU) stay [odds ratio (OR), 1.433; 95% confidence interval (CI), 1.141-1.882; = 0.005], tracheal intubation duration (OR, 1.021; CI, 1.001-1.062; = 0.175), use of a nasogastric feeding tube (OR, 9.131; CI, 1.364-62.289; = 0.021), and Acute Physiology and Chronic Health Evaluation (APACHE)-II C score (OR, 1.709; CI, 1.421-2.148; < 0.001) were selected as risk predictors for dysphagia and included in the nomogram model. The area under the receiver operating characteristic curve was 0.980 (CI, 0.965-0.996) in the training set and 0.971 (0.937-1) in the validation set, with Brier scores of 0.045 and 0.056, respectively. Patients who stay longer in the NICU, have a longer duration of tracheal intubation, require a nasogastric feeding tube, and have higher APACHE-II C scores after neurosurgery are likely to develop dysphagia. This developed model is a convenient and efficient tool for predicting the development of dysphagia.
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http://dx.doi.org/10.3389/fneur.2021.605687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144441PMC
May 2021
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