Publications by authors named "Qian Tang"

255 Publications

Ghrelin ameliorates cardiac fibrosis after myocardial infarction by regulating the Nrf2/NADPH/ROS pathway.

Peptides 2021 Jul 24:170613. Epub 2021 Jul 24.

The affiliated hospital of Shanxi University of Traditional Chinese Medicine, Xianyang, China. Electronic address:

To evaluate the role of ghrelin in cardiac fibrosis after myocardial infarction (MI) and to investigate the underlying mechanisms of ghrelin-regulated Nrf2/NADPH/ROS pathway-mediated cardioprotection, the profile of Nrf2, fibrosis markers, and oxidative stress markers were characterized in a rat model of MI and Angiotensin II (Ang II)-stimulated cardiac fibroblasts (CFs). The effects of ghrelin on cardiac function, fibrosis and oxidative stress were investigated after MI in vivo. The role of ghrelin in CF migration and proliferation was evaluated in Ang II-stimulated CFs in vitro. Inhibition of ghrelin receptors using the antagonist, D-Lys-GHRP-6, in addition to ghrelin was employed in MI and CFs to investigate the direct effect of ghrelin on cardiac fibrosis. Loss function of Nrf2 in CFs was performed to investigate the effect of ghrelin-regulated Nrf2 on oxidative stress and cardiac fibrosis. Ghrelin improved the post-MI cardiac function and reduced cardiac fibrosis. This phenotype is associated with the upregulation of Nrf2 and downregulation of fibrotic proteins, NADPH oxidase and ROS production. In line with in vivo findings, ghrelin attenuated Ang II-stimulated CF migration, proliferation, and oxidative stress in vitro. Inhibition of the ghrelin receptor or knockdown of Nrf2 abolished the beneficial effects of ghrelin on MI or Ang II-stimulated cardiac fibroblasts. In conclusion, ghrelin ameliorates post-MI and Ang II-induced cardiac fibrosis by activating Nrf2, which in turn inhibits the NADPH/ROS pathway.
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http://dx.doi.org/10.1016/j.peptides.2021.170613DOI Listing
July 2021

[Predictive analysis of quality markers of Coptidis Rhizoma based on network pharmacology and multivariate statistical analysis].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(11):2718-2727

State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine Tianjin 301617,China.

Coptidis Rhizoma, as a bulk medicinal material, is in great demand in clinical practice. Its quality is uneven in the market due to the mixture of genuine, counterfeit and adulterants. Therefore, it is particularly important to establish a quality control system for Coptidis Rhizoma. Based on the concept of Chinese medicine quality marker(Q-marker), the potential quality markers of Coptidis Rhizoma were analyzed and predicted from the perspective of chemistry and pharmacology. The sources of the Q-markers of Coptidis Rhizoma were identified by literature retrieval. The potential Q-markers were then screened through the visualization of the "components-targets-pathways" network. High performance liquid chromatography(HPLC) was used to establish a multi-indicator qualitative and quantitative control method featuring fingerprints for 10 batches of Coptidis Rhizoma. A supervised mode of orthogonality partial least squares method-discriminant analysis(OPLS-DA) was used to screen the main marker components that caused differences between groups. The literature review results showed that the alkaloids were the main source of Coptidis Rhizoma Q-markers.The fingerprints of 13 common peaks were successfully established, and berberine, palmatine, berberine and epiberberine were selected as Q-markers of Coptidis Rhizoma, and their contents were determined.Based on the concept of the Q-marker of traditional Chinese medicine, the four components can be selected as the Q-marker of Coptidis Rhizoma after comprehensive consideration. The results of this study are not only conducive to the quality evaluation of Coptidis Rhizoma on the market, but also provide a reference for the overall quality control of Coptidis Rhizoma and lay foundation for the future exploration of the mechanism of Coptidis Rhizoma.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210125.302DOI Listing
June 2021

RNA demethylation increases the yield and biomass of rice and potato plants in field trials.

Nat Biotechnol 2021 Jul 22. Epub 2021 Jul 22.

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

RNA N-methyladenosine (mA) modifications are essential in plants. Here, we show that transgenic expression of the human RNA demethylase FTO in rice caused a more than threefold increase in grain yield under greenhouse conditions. In field trials, transgenic expression of FTO in rice and potato caused ~50% increases in yield and biomass. We demonstrate that the presence of FTO stimulates root meristem cell proliferation and tiller bud formation and promotes photosynthetic efficiency and drought tolerance but has no effect on mature cell size, shoot meristem cell proliferation, root diameter, plant height or ploidy. FTO mediates substantial mA demethylation (around 7% of demethylation in poly(A) RNA and around 35% decrease of mA in non-ribosomal nuclear RNA) in plant RNA, inducing chromatin openness and transcriptional activation. Therefore, modulation of plant RNA mA methylation is a promising strategy to dramatically improve plant growth and crop yield.
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http://dx.doi.org/10.1038/s41587-021-00982-9DOI Listing
July 2021

A Mobile and Self-Powered Micro-Flow Pump Based on Triboelectricity Driven Electroosmosis.

Adv Mater 2021 Jul 16:e2102765. Epub 2021 Jul 16.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

Electroosmotic pumps have been widely used in microfluidic systems. However, traditional high-voltage (HV)-sources are bulky in size and induce numerous accessional reactions, which largely reduce the system's portability and efficiency. Herein, a motion-controlled, highly efficient micro-flow pump based on triboelectricity driven electroosmosis is reported. Utilizing the triboelectric nanogenerator (TENG), a strong electric field can be formed between two electrodes in the microfluidic channel with an electric double layer, thus driving the controllable electroosmotic flow by biomechanical movements. The performance and operation mechanism of this triboelectric electroosmotic pump (TEOP) is systematically studied and analyzed using a basic free-standing mode TENG. The TEOP produces ≈600 nL min micro-flow with a Joule heat down to 1.76 J cm nL compared with ≈50 nL min and 8.12 J cm nL for an HV-source. The advantages of economy, efficiency, portability, and safety render the TEOP a more conducive option to achieve wider applications in motion-activated micro/nanofluidic transportation and manipulation.
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http://dx.doi.org/10.1002/adma.202102765DOI Listing
July 2021

Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens.

Plant Biotechnol J 2021 Jul 15. Epub 2021 Jul 15.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, China.

Sclerotinia sclerotiorum and Botrytis cinerea are typical necrotrophic pathogens that can attack more than 700 and 3000 plant species, respectively, and cause huge economic losses across numerous crops. In particular, the absence of resistant cultivars makes the stem rot because of S. sclerotiorum the major threat of rapeseed (Brassica napus) worldwide along with Botrytis. Previously, we identified an effector-like protein (SsSSVP1) from S. sclerotiorum and a homologue of SsSSVP1 on B. cinerea genome and found that SsSSVP1 could interact with BnQCR8 of rapeseed, a subunit of the cytochrome b-c1 complex. In this study, we found that BnQCR8 has eight homologous copies in rapeseed cultivar Westar and reduced the copy number of BnQCR8 using CRISPR/Cas9 to improve rapeseed resistance against S. sclerotiorum. Mutants with one or more copies of BnQCR8 edited showed strong resistance against S. sclerotiorum and B. cinerea. BnQCR8-edited mutants did not show significant difference from Westar in terms of respiration and agronomic traits tested, including the plant shape, flowering time, silique size, seed number, thousand seed weight and seed oil content. These traits make it possible to use these mutants directly for commercial production. Our study highlights a common gene for breeding of rapeseed to unravel the key hindrance of rapeseed production caused by S. sclerotiorum and B. cinerea. In contrast to previously established methodologies, our findings provide a novel strategy to develop crops with high resistance against multiple pathogens by editing only a single gene that encodes the common target of pathogen effectors.
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http://dx.doi.org/10.1111/pbi.13667DOI Listing
July 2021

Decreased Physiological Serum Total Bile Acid Concentrations in Patients with Type 2 Diabetic Peripheral Neuropathy.

Diabetes Metab Syndr Obes 2021 28;14:2883-2892. Epub 2021 Jun 28.

Southwest Medical University, Luzhou, Sichuan, 646000, People's Republic of China.

Purpose: Bile acids, amphipathic cholesterol metabolites, have been reported to have cytoprotective and neuroprotective effects in humans and animal models. The relationship of physiological serum total bile acid (TBA) levels with diabetic peripheral neuropathy (DPN), however, has not been determined. The purpose of this study was to investigate the relationship between physiological serum TBA and DPN.

Patients And Methods: In total, 856 patients with type 2 diabetes mellitus (T2DM) aged 20-89 years were enrolled in this cross-sectional study. Serum TBA was measured, and its relationship with DPN and other parameters was analyzed.

Results: T2DM patients with DPN had significantly lower serum TBA compared with those without (<0.01). Serum TBA was negatively associated with glycated hemoglobin A1C, plateletcrit, fibrinogen, urine albumin-to-creatinine ratio, vibration perception thresholds, and prevalence of DPN, peripheral arterial disease, and diabetic foot ulceration after adjustment for age, sex, and body mass index (<0.01 or <0.05). A graded association with prevalence of DPN and increase in serum TBA quartiles was observed ( for trend <0.01), and there was an 48.2% decreased risk of DPN in the highest quartile of serum TBA versus the lowest quartile (95% CI 0.299-0.617; =0.000) after multivariate adjustment. Receiver-operating characteristic analysis revealed that the optimal cutoff point of serum TBA to indicate DPN was 2.85 μmol/L (sensitivity 77.6% and specificity 45.6%).

Conclusion: These findings suggest that lower physiological serum TBA level may be associated with the prevalence of DPN in T2DM patients and may be a potential biomarker for DPN.
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http://dx.doi.org/10.2147/DMSO.S313488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254093PMC
June 2021

Association of remnant cholesterol with chronic kidney disease in middle-aged and elderly Chinese: a population-based study.

Acta Diabetol 2021 Jun 28. Epub 2021 Jun 28.

Department of Endocrinology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.

Aims: Limited data regarding the association between remnant cholesterol (RC) and chronic kidney disease (CKD), largely based on an estimated glomerular filtration rate (eGFR) of < 60 mL/min/1.73 m (low eGFR), have yielded inconsistent results, and no report has demonstrated the relationship of RC with CKD [defined as low eGFR and/or albuminuria (defined as urinary albumin-to-creatinine ratio (ACR) ≥ 30 mg/g)] in Chinese general middle-aged and elderly population. Hence, we aimed to investigate the association between RC and CKD in such population.

Methods: In total, 7356 Chinese participants aged ≥ 40 years were recruited from five regional communities in Luzhou city between May 2011 and December 2011. Fasting RC was calculated from the lipid profile measured by standard laboratory procedures. Multivariate logistic regression models were used to evaluate the possible association between RC and CKD.

Results: Participants in the highest quartile of RC had higher body mass index, systolic and diastolic blood pressure, total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), fasting and 2-h postload blood glucose, glycated hemoglobin A1C, prevalence of prediabetes, type 2 diabetes mellitus (T2DM), hypertension, CKD, albuminuria, low eGFR, and lower high-density lipoprotein cholesterol (HDL-C) and eGFR compared with those in the lowest quartile (all P for trend < 0.01). Multivariate logistic regression analysis demonstrated that the risk of CKD gradually increased across RC quartiles (P for trend < 0.01), and participants in the highest quartile of RC were at a significantly increased risk of prevalent CKD compared to those in the lowest quartile in total subjects (odds rate: 1.344, 95% confidence intervals 1.097-1.648, P < 0.01). In subgroup analysis, significant relation between RC level and increased risk of prevalent CKD was detected in women, subjects with overweight/obesity, non-prediabetes, hypertension, normal HDL-C, appropriate and high LDL-C, and without cardiovascular disease (CVD) events after multiple adjustments.

Conclusions: Higher RC is independently associated with increased risk of prevalent CKD, and RC might serve as a new risk biomarker for CKD in a general middle-aged and elderly Chinese population, especially in women, subjects with overweight/obesity, non-prediabetes, hypertension, normal HDL-C, appropriate and high LDL-C, and without CVD events.
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http://dx.doi.org/10.1007/s00592-021-01765-zDOI Listing
June 2021

Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis.

Plant Methods 2021 Jun 22;17(1):63. Epub 2021 Jun 22.

Department of Horticultural Science and Microbial and Plant Genomics Institute, University of Minnesota, St. Paul, MN, USA.

Background: The plant hormone auxin plays a central role in regulation of plant growth and response to environmental stimuli. Multiple pathways have been proposed for biosynthesis of indole-3-acetic acid (IAA), the primary auxin in a number of plant species. However, utilization of these different pathways under various environmental conditions and developmental time points remains largely unknown.

Results: Monitoring incorporation of stable isotopes from labeled precursors into proposed intermediates provides a method to trace pathway utilization and characterize new biosynthetic routes to auxin. These techniques can be aided by addition of chemical inhibitors to target specific steps or entire pathways of auxin synthesis.

Conclusions: Here we describe techniques for pathway analysis in Arabidopsis thaliana seedlings using multiple stable isotope-labeled precursors and chemical inhibitors coupled with highly sensitive liquid chromatography-mass spectrometry (LC-MS) methods. These methods should prove to be useful to researchers studying routes of IAA biosynthesis in vivo in a variety of plant tissues.
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http://dx.doi.org/10.1186/s13007-021-00763-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220744PMC
June 2021

Magnetic Array Assisted Triboelectric Nanogenerator Sensor for Real-Time Gesture Interaction.

Nanomicro Lett 2021 Jan 5;13(1):51. Epub 2021 Jan 5.

Department of Applied Physics, State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, Chongqing University, Chongqing, 400044, People's Republic of China.

In human-machine interaction, robotic hands are useful in many scenarios. To operate robotic hands via gestures instead of handles will greatly improve the convenience and intuition of human-machine interaction. Here, we present a magnetic array assisted sliding triboelectric sensor for achieving a real-time gesture interaction between a human hand and robotic hand. With a finger's traction movement of flexion or extension, the sensor can induce positive/negative pulse signals. Through counting the pulses in unit time, the degree, speed, and direction of finger motion can be judged in real-time. The magnetic array plays an important role in generating the quantifiable pulses. The designed two parts of magnetic array can transform sliding motion into contact-separation and constrain the sliding pathway, respectively, thus improve the durability, low speed signal amplitude, and stability of the system. This direct quantization approach and optimization of wearable gesture sensor provide a new strategy for achieving a natural, intuitive, and real-time human-robotic interaction.
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http://dx.doi.org/10.1007/s40820-020-00575-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187499PMC
January 2021

Downregulation of ACE2 expression by SARS-CoV-2 worsens the prognosis of KIRC and KIRP patients via metabolism and immunoregulation.

Int J Biol Sci 2021 10;17(8):1925-1939. Epub 2021 May 10.

School of Pharmacy, Jinan University, Guangzhou 510630, China.

Angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) allow entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into host cells and play essential roles in cancer therapy. However, the functions of ACE2 and TMPRSS2 in kidney cancer remain unclear, especially as kidneys are targets for SARS-CoV-2 infection. UCSC Xena project, the Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) databases (GSE30589 and GSE59185) were searched for gene expression in human tissues, gene expression data, and clinical information. Several bioinformatics methods were utilized to analyze the correlation between ACE2 and TMPRSS2 with respect to the prognosis of kidney renal clear cell carcinoma (KIRC) and kidney renal papillary cell carcinoma (KIRP). ACE2 expression was significantly upregulated in tumor tissue, while its downregulation was associated with low survival in KIRC and KIRP patients. TMPRSS2 was downregulated in KIRC and KIRP, and its expression was not correlated with patient survival. According to clinical risk factor-based prediction models, ACE2 exhibits predictive accuracy for kidney cancer prognosis and is correlated with metabolism and immune infiltration. In an animal model, ACE2 expression was remarkably downregulated in SARS-CoV-2-infected cells compared to in the control. ACE2 expression is highly correlated with various metabolic pathways and is involved in immune infiltration.it plays a crucial role than TMPRSS2 in diagnosing and prognosis of kidney cancer patients. The overlap in ACE2 expression between kidney cancer and SARS-CoV-2 infection suggests that patients with KIRC or KIRP are at high risk of developing serious symptoms.
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http://dx.doi.org/10.7150/ijbs.57802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193256PMC
June 2021

Disruption of postnatal neurogenesis and adult-stage suppression of synaptic plasticity in the hippocampal dentate gyrus after developmental exposure to sterigmatocystin in rats.

Toxicol Lett 2021 Oct 11;349:69-83. Epub 2021 Jun 11.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan. Electronic address:

Exposure to sterigmatocystin (STC) raises concerns on developmental neurological disorders. The present study investigated the effects of maternal oral STC exposure on postnatal hippocampal neurogenesis of offspring in rats. Dams were exposed to STC (1.7, 5.0, and 15.0 ppm in diet) from gestational day 6 until day 21 post-delivery (weaning), and offspring were maintained without STC exposure until adulthood on postnatal day (PND) 77, in accordance with OECD chemical testing guideline Test No. 426. On PND 21, 15.0-ppm STC decreased type-3 neural progenitor cell numbers in the subgranular zone (SGZ) due to suppressed proliferation. Increased γ-H2AX-immunoreactive () cell numbers in the SGZ and Ercc1 upregulation and Brip1 downregulation in the dentate gyrus suggested induction of DNA double-strand breaks in SGZ cells. Upregulation of Apex1 and Ogg1 and downregulation of antioxidant genes downstream of NRF2-Keap1 signaling suggested induction of oxidative DNA damage. Increased p21 SGZ cell numbers and suppressed cholinergic signaling through CHRNB2-containing receptors in GABAergic interneurons suggested potential neurogenesis suppression mechanisms. Multiple mechanisms involving N-methyl-d-aspartate (NMDA) receptor-mediated glutamatergic signaling and various GABAergic interneuron subpopulations, including CHRNA7-expressing somatostatin interneurons activated by BDNF-TrkB signaling, may be involved in ameliorating the neurogenesis. Upregulation of Arc, Ptgs2, and genes encoding NMDA receptors and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors suggested synaptic plasticity facilitation. On PND 77, ARC granule cells decreased, and Nos2 was upregulated following 15.0 ppm STC exposure, suggesting oxidative stress-mediated synaptic plasticity suppression. Inverse pattern in gene expression changes in vesicular glutamate transporter isoforms, Slc17a7 and Slc17a6, from weaning might also be responsible for the synaptic plasticity suppression. The no-observed-adverse-effect level of maternal oral STC exposure for offspring neurogenesis was determined to be 5.0 ppm, translating to 0.34-0.85 mg/kg body weight/day.
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http://dx.doi.org/10.1016/j.toxlet.2021.06.006DOI Listing
October 2021

Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells.

J Agric Food Chem 2021 Jun 7;69(24):6810-6819. Epub 2021 Jun 7.

In the dairy industry, glutamine (Gln) is often used as a feed additive to increase milk yield and quality; however, the molecular regulation underneath needs further clarification. Here, with bovine mammary epithelial cells (BMECs), the effects and mechanisms of Gln on cell growth and casein synthesis were assessed. When Gln was added or depleted from BMECs, both cell growth and β-casein (CSN2) expression were increased or decreased, respectively. Overexpressing or inhibiting the mechanistic target of rapamycin (mTOR) revealed that Gln regulated cell growth and CSN2 synthesis through the mTORC1 pathway. A similar intervention of ADP-ribosylation factor 1 (Arf1) uncovered that Gln activated the mTORC1 pathway through Arf1. We next observed that both guanine nucleotide exchange factors, Cytohesin-1/2/3 (CYTH1/2/3, CYTHs) and ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), interacted with Arf1. Inhibiting CYTHs or ARFGAP1 showed that Gln supplement or depletion activated or inactivated Arf1 through CYTHs or ARFGAP1, respectively. Collectively, this study demonstrated that Gln positively regulated cell growth and casein synthesis in BMECs, which works through the CYTHs/ARFGAP1-Arf1-mTORC1 pathway. These results greatly enhanced current understanding regarding the regulation of the mTOR pathway and provided new insights for the processes of cell growth and casein synthesis by amino acids, particularly Gln.
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http://dx.doi.org/10.1021/acs.jafc.1c02223DOI Listing
June 2021

Assessment of a novel aminated magnetic adsorbent with excellent adsorption capacity for dyes and drugs.

J Environ Manage 2021 Sep 1;293:112809. Epub 2021 Jun 1.

College of Civil Engineering, Sichuan Agricultural University, Chengdu, 611830, China.

Dyes and drugs with high toxicity and low biodegradability pose risk to human health and ecological security, and should be purified efficiently from effluents before discharge. Traditional adsorbents are limited by the insufficient active adsorption sites and low stability. In this study, a novel aminated magnetic adsorbent (MCTs) was fabricated via two cross-linking steps using chitosan and triethylenetetramine to fill the gaps between current adsorbent and performance requirements. The morphological and physicochemical characteristics of the as-prepared MCTs were determined and identified with the aid of several characterization techniques. The adsorption performance of dyes and drugs was also investigated and represented by their adsorption capacities. In particular, the adsorption capacities of Congo Red, Chicago Sky Blue, Reactive Brilliant Red, and Ibuprofen were 583.11, 465.01, 403.12, and 291.71 mg/g, respectively. They also remained at around 80% after four reuse cycles. MCTs were adsorbed via a monolayer spontaneous chemical reaction, and hydrogen bonding and electrostatic interaction were the dominant adsorption mechanisms. These results demonstrated that the preparation of MCTs via two cross-linking steps enhanced the adsorbents' adsorption capacity, reusability, and stability. They provided a new perspective for the preparation of high-efficient adsorbents and the purification of dye- and drug-polluted wastewater.
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http://dx.doi.org/10.1016/j.jenvman.2021.112809DOI Listing
September 2021

Erratum: Microporous polysaccharide multilayer coated BCP composite scaffolds with immobilised calcitriol promote osteoporotic bone regeneration both and : Erratum.

Theranostics 2021 20;11(13):6524-6525. Epub 2021 Apr 20.

Key Laboratory of Orthopaedics of Zhejiang Province, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109, Xueyuanxi road, 325027 Wenzhou, China.

[This corrects the article DOI: 10.7150/thno.29566.].
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http://dx.doi.org/10.7150/thno.61641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120221PMC
April 2021

Effects of chromium stress on the rhizosphere microbial community composition of Cyperus alternifolius.

Ecotoxicol Environ Saf 2021 Apr 29;218:112253. Epub 2021 Apr 29.

College of Eco-environment Engineering, Guizhou Minzu University, Guiyang 550025, China; Institute of Karst Wetland Ecology, Guizhou Minzu University, Guiyang 550025, China.

Wetland plants are often used as the main body of soil, and the rhizosphere is a hot spot migration and transformation. Response mechanism to rhizosphere microorganisms on chromium(Cr) stressing could help improve the phytoremediation system. Cyperus alternifolius(CA) is selected as the research object by Cr-stress treatments and uncontaminated treatments with different cultivated pattern, included sole cultivated pattern(CAI), two-cultivated pattern (CAII), three-cultivated pattern (CAIII), and the un-planted blank samples (CK). 16s rRNA gene sequencing and metagenomic sequencing are performed to measure rhizosphere microbial community. And Five common enzymes in rhizosphere soils were observed: β-1,4-glucosidase (BG), β-N-acetylglucosaminidase (NAG), β-1,4-xylosidase (BX), cellobiohydrolase (CBH) and Leucine amino peptidase (LAP) in the rhizosphere. The results show that Gammaproteobacteria, Actinobacteria, Alphaproteobacteria, Gemmatimonadetes, Deltaproteobacteria are top five (63.97%) of the total sequence number. Wetland plants enriched a large amount of soil Cr in themselves, and the rhizosphere microorganisms don't show significant difference in community structure after affecting. 10.48% variation of microbial community is caused by Cr-stress. Acidovorax showed a great potential for chromium resistance. BX involvement in tolerance processes indirectly affects microbial communities (P < 0.01), there is a strong linear relationship between enzyme activity and the plants accumulating Cr and microbial community within 15.58% variant. The material accumulation and microbial quantity of CAIII are relatively low, but high biodiversity remains after affecting. These results provide references for in-depth understanding of rhizosphere microbial response to heavy metal pollution in wetland phytoremediation and interaction between wetland plants and rhizosphere microorganisms.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112253DOI Listing
April 2021

Upregulation of polymeric immunoglobulin receptor expression in flounder (Paralichthys olivaceus) gill cells by cytokine tumor necrosis factor-α via activating PI3K and NF-κB signaling pathways.

Mol Immunol 2021 Jul 23;135:170-182. Epub 2021 Apr 23.

Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao, 266003, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, PR China. Electronic address:

The polymeric immunoglobulin receptor (pIgR) transports secretory immunoglobulins across mucosal epithelial cells into external secretions, playing critical roles in mucosal surface defenses, but the regulation mechanism of pIgR expression is not clarified in teleost fish. In this study, the dynamic changes of flounder (Paralichthys olivaceus) pIgR (fpIgR) and pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) mRNA expression in mucosal tissues were first analyzed post inactivated Vibrio anguillarum immunization, and increased production of TNF-α was found to correlate with increased expression of fpIgR. To determine that cytokine TNF-α influenced fpIgR expression, following confirming that natural fpIgR expressed on flounder gill (FG) cells, FG cells were incubated with various concentrations of recombinant TNF-α for different time, the results showed that the expressions of fpIgR were significantly upregulated at gene and protein levels in a dose-dependent and time-dependent manner, and similar change trend was observed for free secretory component (SC) secreted by fpIgR into the culture supernatant. After FG cells were treated with TNF-α, specific phosphoinositide 3-kinase (PI3K) inhibitor wortmannin, nuclear factor kappa-B (NF-κB) inhibitor Bay11-7082, and the mixtures of TNF-α and wortmannin / Bay11-7082 respectively, the fpIgR protein and mRNA levels, together with SC secretion, obviously decreased in wortmannin- and Bay11-7082-treated cells compared with the untreated control, and cotreatment with wortmannin / Bay11-7082 plus TNF-α resulted in lower expression compared with that upon treatment with TNF-α alone, indicating that the inhibition of PI3K and NF-κB both blocked the ability of TNF-α to increase cellular fpIgR and SC levels. Furthermore, the gene expressions of PI3K and NF-κB were upregulated and present a tendency to increase first and then decrease after TNF-α treatment of FG cells; However, the expression of PI3K mRNA was inhibited significantly by wortmannin but not by Bay11-7082, and the expression of NF-κB mRNA was suppressed obviously by Bay11-7082 but not by wortmannin, suggesting that inhibition of PI3K or NF-κB had no influence on each other. All these results collectively revealed that TNF-α could transcriptionally upregulate fpIgR expression and SC production, and this TNF-α-induced pIgR expression was regulated by complex mechanisms that involved PI3K and NF-κB signaling pathways, which provided evidences for pro-inflammatory cytokine TNF-α acting as a regulator in pIgR expression and better understanding of regulation mechanism of pIgR expression in teleost fish.
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http://dx.doi.org/10.1016/j.molimm.2021.04.011DOI Listing
July 2021

Multi-Mode Reconfigurable DNA-Based Chemical Reaction Circuits for Soft Matter Computing and Control.

Angew Chem Int Ed Engl 2021 06 1;60(27):15013-15019. Epub 2021 Jun 1.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.

Developing smart material systems for performing different tasks in diverse environments remains challenging. Here, we show that by integrating stimuli-responsive soft materials with multi-mode reconfigurable DNA-based chemical reaction circuits (D-CRCs), it can control size change of microgels with multiple reaction pathways and adapt expansion behaviors to meet diverse environments. We first use pH-responsive intramolecular conformational switches for regulating DNA strand displacement reactions (SDRs). The ability to regulate SDRs with tunable pH-dependence allows to build dynamic chemical reaction networks with diverse reaction pathways. We confirm that the designed DNA switching circuits are reconfigurable at different pH and perform different logic operations, and the swelling of DNA switching circuit-integrated microgel systems can be programmably directed by D-CRCs. Our approach provides insight into building smart responsive materials and fabricating autonomous soft robots.
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http://dx.doi.org/10.1002/anie.202102169DOI Listing
June 2021

Induction of cellular senescence as a late effect and BDNF-TrkB signaling-mediated ameliorating effect on disruption of hippocampal neurogenesis after developmental exposure to lead acetate in rats.

Toxicology 2021 05 20;456:152782. Epub 2021 Apr 20.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan. Electronic address:

Lead (Pb) exposure causes cognitive deficits in children. The present study investigated the effect of developmental exposure to Pb acetate (PbAc) on postnatal hippocampal neurogenesis. Pregnant rats were administered drinking water containing 0, 2000, or 4000 ppm PbAc from gestational day 6 until day 21 post-delivery (weaning), and offspring were maintained without PbAc exposure until adulthood on postnatal day (PND) 77. There was a dose-related accumulation of Pb in the offspring brain at weaning, while Pb was mainly excreted in adulthood. In the hippocampus, metallothionein I/II immunoreactive glia were increased through adulthood as a neuroprotective response to accumulated Pb, accompanied by increased astrocyte and microglia numbers in adulthood, suggesting sustained neural damage. Gene expression changes suggested elevated oxidative stress at weaning and suppression of the antioxidant system in adulthood, as well as continued neuroinflammatory responses. At weaning, granule cell apoptosis was increased and numbers of type-3 neural progenitor cells (NPCs) were decreased. By contrast, type-2a and type-2b NPCs were increased, suggesting suppressed differentiation to type-3 NPCs. In adulthood, there were increased numbers of immature granule cells. In the hilus of the dentate gyrus, somatostatin interneurons were increased at weaning, while calbindin-D-29K interneurons were increased throughout adulthood, suggesting a strengthened interneuron regulatory system against the suppressed differentiation at weaning. In the dentate gyrus, Bdnf, Ntrk2, and Chrna7 gene expression were upregulated and numbers of hilar TrkB interneurons increased at weaning. These findings suggest activation of BDNF-TrkB signaling to increase somatostatin interneurons and promote cholinergic signaling, thus increasing later production of immature granule cells. In adulthood, Pcna and Apex1 gene expression were downregulated and Chek1 and cyclin-dependent kinase inhibitor expression were upregulated. Furthermore, there was an increase in γ-H2AX SGZ cells, suggesting induction of cellular senescence of SGZ cells due to Pb genotoxicity.
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http://dx.doi.org/10.1016/j.tox.2021.152782DOI Listing
May 2021

A 28-day repeated oral dose toxicity study of enniatin complex in mice.

J Toxicol Sci 2021 ;46(4):157-165

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology.

Enniatins are so-called "emerging mycotoxins" that commonly occur in milligrams per kilogram levels in grains and their derived products, as well as in fish, dried fruits, nuts, spices, cocoa, and coffee. The present study investigated the 28-day repeated oral dose toxicity of enniatin complex in CD1(ICR) mice. Enniatin B, enniatin B1, and enniatin A1 at a ratio of 4:4:1 were administered to male and female mice at doses of 0 (vehicle controls), 0.8, 4, and 20 mg/kg body weight/day. In life parameters did not change during the study period, with the exception of slight reductions in food consumption in male mice administered 4 and 20 mg/kg and in female mice administered 20 mg/kg. Body and organ weights did not change, and no alterations in hematology, blood biochemistry, or histopathology parameters were observed at the end of the administration period. Thus, we determined that the no-observed-adverse-effect level of enniatin complex was 20 mg/kg/day for both sexes under the present experimental conditions.
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http://dx.doi.org/10.2131/jts.46.157DOI Listing
January 2021

A hollow visible-light-responsive surface molecularly imprinted polymer for the detection of chlorpyrifos in vegetables and fruits.

Food Chem 2021 Sep 23;355:129656. Epub 2021 Mar 23.

The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address:

A visible-light-responsive azobenzene derivative, 3,5-dichloro-4-((2,6-dichloro-4-(methacryloyloxy)phenyl)diazenyl)benzoic acid, was synthesized and used as the functional monomer to fabricate a visible-light-responsive core-shell structured surface molecularly imprinted polymer ([email protected]). After removal of the sacrificial PS-co-PMAA core, a hollow structured surface molecularly imprinted polymer (HVSMIP) was obtained. Both the [email protected] and HVSMIP were used for the detection of chlorpyrifos, a moderately toxic organophosphate pesticide. They exhibited good visible-light-responsive properties (550 nm for trans→cis and 440 nm for cis→trans isomerization for an azobenzene chromophore) in ethanol/water (9:1, v/v). Compared with the [email protected], the HVSMIP had a larger surface area, pore volume, binding capacity, imprinting effect, maximum chemical binding capacity, dissociation constant, and photo-isomerization rate. The HVSMIP was applied to detect trace chlorpyrifos in fruit and vegetable samples. This was achieved by measuring the trans→cis rate constant of the HVSMIP in the sample solution, with good recoveries, low relative standard deviations, and a low detection limit.
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http://dx.doi.org/10.1016/j.foodchem.2021.129656DOI Listing
September 2021

The Neural Stem Cell Properties of PKD2L1 Cerebrospinal Fluid-Contacting Neurons .

Front Cell Neurosci 2021 15;15:630882. Epub 2021 Mar 15.

Department of Orthopedics Traumatic, The Affiliated Hospital of Guizhou Medical University, Guiyang City, China.

Cerebrospinal fluid-touching neurons (CSF-cNs) exist in the region surrounding the central canal of the spinal cord, which locate in the adult neurogenic niche. Previous research showed that CSF-cNs expressed the molecular markers of immature neural cells . Here, we explored the potential of CSF-cNs as neural stem cell . We first found that PKD2L1 CSF-cNs, isolating by FACS using the molecular marker PKD2L1 of CSF-cNs, expressed neural stem cells markers like Nestin, Sox2, and GFAP by immunofluorescence staining. PKD2L1 CSF-cNs were able to form neurospheres and passaged . Immunofluorescence staining showed that the neurospheres forming by PKD2L1 CSF-cNs also expressed neural stem cell markers Nestin, Sox2 and GFAP. The neurospheres expressed proliferation markers Ki67 and PCNA by immunofluorescence staining, indicating that the neurospheres forming by PKD2L1 CSF-cNs were proliferative. The neurospheres, forming by CSF-cNs, had the ability of differentiation into neurons, astrocytes, and oligodendrocytes. Collectively, our data suggested that PKD2L1 CSF-cNs have the properties of neural stem cells and may provide a promising approach for the repair of spinal cord injury.
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http://dx.doi.org/10.3389/fncel.2021.630882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005615PMC
March 2021

Genome-wide markers redeem the lost identity of a heavily managed gamebird.

Proc Biol Sci 2021 03 24;288(1947):20210285. Epub 2021 Mar 24.

Department of Biology, University of Pisa, Via A. Volta 4, 56126 Pisa, Italy.

Heavily managed wildlife may suffer from genetic homogenization and reshuffling of locally adapted genotypes with non-native ones. This phenomenon often affects natural populations by reducing their evolutionary potential and speeding up the ongoing biodiversity crisis. For decades, the red-legged partridge (), an intensively managed gamebird of conservation concern and considerable socio-economic importance, has been subjected to extensive releases of farm-reared hybrids with the chukar partridge () and translocations irrespective of subspecific affinity. These practices have led to serious concerns that the genetic integrity and biogeographic structure of most red-legged partridge populations are irreversibly affected, as suggested by previous studies based on few genetic markers. Using over 168 000 genome-wide loci and a sampling across the entire range, we detected unexpectedly limited and spatially uneven chukar introgression as well as significant intraspecific structure. We demonstrate that species widely feared to have irretrievably lost their genetic identity are likely to be much less affected by unsuitable management practices than previously assumed. Our results spell the need for a radical re-think on animal conservation, possibly restoring native status to populations long treated as compromised. Our study exemplifies how the application of innovative conservation-genomic methods is key to solving wildlife management problems dealing with introgressive hybridization worldwide.
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http://dx.doi.org/10.1098/rspb.2021.0285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059960PMC
March 2021

[Contributors to Air Pollutant Emission Changes in Autumn and Winter in Beijing-Tianjin-Hebei and Surrounding Areas].

Huan Jing Ke Xue 2021 Apr;42(4):1591-1599

School of Environment, Tsinghua University, Beijing 100084, China.

Based on the air pollution emission inventory technical methodology, this study conducted a quantitative analysis on the changes in major air pollutant emissions in Beijing-Tianjin-Hebei and its surrounding areas from the 'New Year Haze' in the autumn and winter of 2016-2017 to the 'Pandemic Haze' in the autumn and winter of 2019-2020. The contributions of the implementation of air pollution prevention and control policies and the COVID-19 pandemic to major air pollutant emission reductions were studied, and their impacts on the regional air quality under adverse meteorological conditions were simulated using an air quality model. The results showed that from the 'New Year Haze' in Dec 2016-Jan 2017 to the 'Pandemic Haze' in Jan-Feb 2020, the major air pollutant emissions in the region had dropped by approximately 50%, and the average concentration of PM was potentially reduced by more than 40% under adverse meteorological conditions. The most effective emission reduction measures included the clean heating project and raising the standards in key industrial sectors, such as the iron and steel industry, coal-fired boilers, and power plants, which contributed 67.1% and 53.4% of the emission reductions in SO and PM, respectively. The COVID-19 pandemic predominantly affected the mobile sources and light industry, which contributed 71.9% and 68.2% of the emission reductions in NO and VOCs, respectively. The implementation of air pollution prevention and control policies contributed substantially to the improvement of regional air quality, which effectively reduced the intensity and extent of the heavy pollution process under unfavorable meteorological conditions. The regional average PM concentration was reduced by 26%, and the number of days experiencing heavy pollution decreased by 44%. Due to the impacts of the COVID-19 pandemic, the average PM concentration in the region was reduced by an additional 24%, and the duration and extent of heavy pollution decreased even further.
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http://dx.doi.org/10.13227/j.hjkx.202007218DOI Listing
April 2021

Distribution characteristics of bioaerosols inside pig houses and the respiratory tract of pigs.

Ecotoxicol Environ Saf 2021 Apr 5;212:112006. Epub 2021 Feb 5.

Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Particulate matter (PM) is a carrier of many substances. Microorganisms are vital constituents contained in PM, and their varieties and concentrations are closely connected to human health and animal production. This study aimed to investigate the distribution characteristics of bioaerosols inside a pig house and in the respiratory tract of pigs. Environmental indices inside a nursery pig house were monitored in winter, including temperature, relative humidity, total suspended particulate (TSP), PM, PM, NH, CO, CO and NO. The concentrations of airborne culturable bacteria, fungi and Escherichia coli were detected. Then, 16S rRNA sequencing technology was applied to identify different-sized bioaerosols and bacteria in the respiratory tract of piglets. The results showed that the concentration of airborne culturable bacteria inside the pig house was significantly higher than that outside, and no significant difference was found among culturable fungi and Escherichia coli. The 16S rRNA results showed that the bacterial aerosols presented high similarity to the bacteria in the respiratory tract of piglets. The airborne bacterial aerosols within the size range of 1.1-3.3 µm showed high similarity to the bacteria in the lower respiratory tract (bronchus and lung) of piglets. In addition, four potential pathogenic bacterial genera (Escherichia-Shigella, Streptococcus, Acinetobacter, Pseudomonas) were identified both in the bacterial aerosols and the respiratory tract of piglets. These results will provide a significant scientific basis for exploring the potential risk of aerosols from animal houses to human and animal health.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112006DOI Listing
April 2021

Wearable triboelectric sensors for biomedical monitoring and human-machine interface.

iScience 2021 Jan 5;24(1):102027. Epub 2021 Jan 5.

Department of Applied Physics, Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, Chongqing University, Chongqing 400044, P.R. China.

A growing advocacy of healthy and quality life makes wearable electronics spring up. Triboelectric nanogenerator (TENG) has developed as an energy harvesting technology and as an advanced sensor technology in wearable electronics. The triboelectric sensor (TS) is sensitive to the mechanical motion and driven by the motion itself. Therefore, TS is capable of monitoring certain vital signs and kinds of movements of human body. Based on these monitoring, novel human-machine interfaces (HMIs) can be established. In this review, a comprehensive overview of some key progresses in this field over last 5 years are presented. Several main aspects of biomedical monitoring based on TSs are classified: pulse/cardiac/micro-motion, respiration/airflow/vibration, and pressure/tactile/body movement. The major types of HMIs taking these biomedical monitoring as basis are introduced accordingly: eye movement, voice/auditory, gesture/joint movement, and touch/tactile based HMIs. Finally, the current limitations and future trends are put forward for biomedical monitoring and HMIs based on TSs.
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http://dx.doi.org/10.1016/j.isci.2020.102027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820136PMC
January 2021

Impacts of meteorology and emission variations on the heavy air pollution episode in North China around the 2020 Spring Festival.

Sci China Earth Sci 2021 Jan 12:1-11. Epub 2021 Jan 12.

Center of Air Modeling and Systems Analysis, Chinese Academy for Environmental Planning, Beijing, 100012 China.

Based on the Weather Research and Forecasting model and the Models-3 community multi-scale air quality model (WRF-CMAQ), this study analyzes the impacts of meteorological conditions and changes in air pollutant emissions on the heavy air pollution episode occurred over North China around the 2020 Spring Festival (January to Februray 2020). Regional reductions in air pollutant emissions required to eliminate the PM heavy pollution episode are also quantified. Our results found that meteorological conditions for the Beijing-Tianjin-Hebei and surrounding "2+26" cities are the worst during the heavy pollution episode around the 2020 Spring Festival as compared with two other typical heavy pollution episodes that occurred after 2015. However, because of the substantial reductions in air pollutant emissions in the "2+26" cities in recent years, and the 32% extra reduction in emissions during January to February 2020 compared with the baseline emission levels of the autumn and winter of 2019 to 2020, the maximum PM level during this heavy pollution episode around the 2020 Spring Festival was much lower than that in the other two typical episodes. Yet, these emission reductions are still not enough to eliminate regional heavy pollution episodes. Compared with the actual emission levels during January to February 2020, a 20% extra reduction in air pollutant emissions in the "2+26" cities (or a 45% extra reduction compared with baseline emission levels of the autumn and winter of 2019 to 2020) could help to generally eliminate regionwide severe pollution episodes, and avoid heavy pollution episodes that last three or more consecutive days in Beijing; a 40% extra reduction in emissions (or a 60% extra reduction compared with baseline emission levels of the autumn and winter of 2019 to 2020) could help to generally eliminate regionwide and continuous heavy pollution episodes. Our analysis finds that during the clean period after the heavy pollution episode around the 2020 Spring Festival, the regionwide heavy pollution episode would only occur with at least a 10-fold increase in air pollutant emissions.
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http://dx.doi.org/10.1007/s11430-020-9683-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807214PMC
January 2021

CircBACH1/let-7a-5p axis enhances the proliferation and metastasis of colorectal cancer by upregulating CREB5 expression.

J Gastrointest Oncol 2020 Dec;11(6):1186-1199

The Fourth Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai, China.

Background: In this study, we investigated the influences of circBACH1 on the proliferation, metastasis, migration, and apoptosis of human colorectal cancer LoVo cells and explored the molecular mechanism of its effect to guide the clinical diagnosis, treatment, and follow-up of colorectal cancer.

Methods: The expression of circBACH1 in colorectal cancer cells was measured to determine the high expression of BACH1 in colorectal cancer (CRC). LoVo was selected for a follow-up experiment. Then, quantificational reverse transcription-polymerase chain reaction (qRT-PCR) and biotinylated let-7a-5p probes were used to confirm that the expression of let-7a-5p was lowered in colorectal cancer, and let-7a-5p was the downstream target of BACH1 in CRC. Cell counting Kit-8 (CCK-8), Transwell, and wound repair experiments confirmed that BACH1 augmented the proliferation, migration, and metastasis of CRC by regulating let-7a-5p. The apoptosis rate was measured by flow cytometry. It was concluded that BACH1 inhibited apoptosis by regulating let-7a-5p in CRC. The results of the bioinformatics analysis showed that CREB5 was overexpressed in CRC by qRT-PCR and Western blot. The results of qRT-PCR, CCK-8 assay, Transwell assay, and flow cytometry showed that let-7a-5p inhibited the proliferation, migration, and invasion of CRC cells through targeting CREB5 and augmented cell apoptosis. According to tumor growth and the determination of CREB5 by Western blot, BACH1 can affect the proliferation of CRC cells through CREB5.

Results: Overall, our study confirmed that BACH1 and CREB5 increased, while the expression of let-7a-5p was lowered in colorectal cancer cells. These different expressions enhance the proliferation, metastasis, and migration of colorectal cancer cells and inhibit colorectal cancer cells' apoptosis.

Conclusions: Our study clearly illustrates the molecular mechanism of circBACH1 acting on colorectal cancer, which can be used as a therapeutic target to augment colorectal cancer treatment.
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http://dx.doi.org/10.21037/jgo-20-498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807282PMC
December 2020

Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation.

Korean J Pain 2021 Jan;34(1):47-57

Key Laboratory of Neuroscience, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, China.

Background: Lumbar disc herniation (LDH) is a common cause of radicular pain, but the mechanism is not clear. In this study, we investigated the engagement of toll-like receptor 4 (TLR4) and the nuclear factor-kappa B (NF-κB) in radicular pain and its possible mechanisms.

Methods: An LDH model was induced by autologous nucleus pulposus (NP) implantation, which was obtained from coccygeal vertebra, then relocated in the lumbar 4/5 spinal nerve roots of rats. Mechanical and thermal pain behaviors were assessed by using von Frey filaments and hotplate test respectively. The protein level of TLR4 and phosphorylated-p65 (p-p65) was evaluated by western blotting analysis and immunofluorescence staining. Spinal microglia activation was evaluated by immunofluorescence staining of specific relevant markers. The expression of pro- and anti-inflammatory cytokines in the spinal dorsal horn was measured by enzyme linked immunosorbent assay.

Results: Spinal expression of TLR4 and p-NF-κB (p-p65) was significantly increased after NP implantation, lasting up to 14 days. TLR4 was mainly expressed in spinal microglia, but not astrocytes or neurons. TLR4 antagonist TAK242 decreased spinal expression of p-p65. TAK242 or NF-κB inhibitor pyrrolidinedithiocarbamic acid alleviated mechanical and thermal pain behaviors, inhibited spinal microglia activation, moderated spinal inflammatory response manifested by decreasing interleukin (IL)-1β, IL-6, tumor necrosis factor-α expression and increasing IL-10 expression in the spinal dorsal horn.

Conclusions: The study revealed that TLR4/NF-κB pathway participated in radicular pain by encouraging spinal microglia activation and inflammatory response.
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http://dx.doi.org/10.3344/kjp.2021.34.1.47DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783850PMC
January 2021

Well-dispersed platelet lysate entrapped nanoparticles incorporate with injectable PDLLA-PEG-PDLLA triblock for preferable cartilage engineering application.

Biomaterials 2021 01 15;268:120605. Epub 2020 Dec 15.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Shanghai Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic address:

Platelet lysate (PL) as a cost-effective cocktail of growth factors is an emerging ingredient in regenerative medicine, especially in cartilage tissue engineering. However, most studies fail to pay attention to PL's intrinsic characteristics and incorporate it directly with scaffolds or hydrogels by simple mixture. Currently, the particle size distribution of PL was determined to be scattered. Directly introducing PL into a thermosensitive poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PLEL) hydrogel disturbed its sol-gel transition. Electrostatic self-assembly heparin (Hep) and ε-poly-l-lysine (EPL) nanoparticles (NPs) were fabricated to improve the dispersity of PL. Such PL-NPs-incorporated PLEL gels retained the initial gelling capacity and showed a long-term PL-releasing ability. Moreover, the PL-loaded composite hydrogels inhibited the inflammatory response and dedifferentiation of IL-1β-induced chondrocytes. For in vivo applications, the [email protected] system ameliorated the early cartilage degeneration and promoted cartilage repair in the late stage of osteoarthritis. RNA sequencing analysis indicated that PL's protective effects might be associated with modulating hyaluronan synthase 1 (HAS-1) expression. Taken together, these results suggest that well-dispersed PL by Hep/EPL NPs is a preferable approach for its incorporation into hydrogels and the constructed [email protected] system is a promising cell-free and stepwise treatment option for cartilage tissue engineering.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120605DOI Listing
January 2021

Exploring the perspective of nano-TiO in hydrophobic modified cationic flocculant preparation: Reaction kinetics and emulsified oil removal performance.

Chemosphere 2021 Jan 23;263:128066. Epub 2020 Aug 23.

College of Civil Engineering, Sichuan Agricultural University, Chengdu, 611830, China; Sichuan Higher Education Engineering Research Center for Disaster Prevention and Mitigation of Village Construction, Department of Municipal Engineering, Chengdu, 611830, China.

To reduce the polymerization difficulty of hydrophobic modified copolymers, a hydrophobic modified cationic flocculant was fabricated using nano-TiO as initiator with acrylamide (AM) and methyl acryloxyethyl dimethyl benzyl ammonium chloride (DML) as monomers, and named it PAD. The copolymers were characterized by scanning electron microscopy (SEM), nuclear magnetic resonance (H NMR), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TG). Results verified that PAD was synthesized successfully and nano-TiO was more conducive to DML grafting than traditional photo-initiators. Reaction kinetics demonstrated that the polymerization process was a typical precipitation polymerization initiated by free radicals. Flocculation performance of flocculant on simulated emulsified oil was evaluated and optimized. The simulation results indicated that the flocculation performance of PAD was superior to traditional flocculant, which was attributed to the higher content of DML in PAD. The maximum removal rate of emulsified oil could reach 92.10%, and the corresponding turbidity removal rate was 93.54%. Further, the mechanism studies suggested that the removal of emulsified oil was realized by the synergistic effects of electric neutralization, demulsification, hydrophobic association and adsorption bridging. The findings of this study showed that nano-TiO exhibited a promising prospect in the field of polymer-initiated polymerization.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128066DOI Listing
January 2021
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