Publications by authors named "Qian Sun"

1,023 Publications

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Construction of Digital Platform of Religious and Cultural Resources Using Deep Learning and Its Big Data Analysis.

Authors:
Qian Sun

Comput Intell Neurosci 2022 30;2022:4258577. Epub 2022 Jul 30.

School of Political Science and Public Administration, Shandong University, Qingdao 266237, China.

This article analyzes the difficulties associated with the preservation and transmission of religious cultural resources and the difficulties encountered in the new development environment and background. It does so in light of the current state of religious, cultural resources. The protection, growth, and use of religious and cultural resources against the backdrop of the digital era are elaborated upon and critically analyzed in this article. Based on the foregoing discussion, this article conducts a thorough analysis of the development of a digital platform for religious and cultural resources and its big data analysis, and it also suggests an image feature extraction algorithm based on DL. This article develops a clustering CNN based on the network with PCA vector as convolution kernel, which clusters small images and computes principal component vectors according to categories, generating multiple groups of convolution kernels to extract more features so that the input image can select feature extractors adaptively. Simulation and comparative analysis are used in this article to confirm the algorithm's effectiveness. Compared to the conventional NN algorithm, simulation results indicate that this algorithm is more accurate, with a maximum accuracy of about 95.14 percent. It has some reference value for the research that will be done in relation to the creation of the next digital platform for religious and cultural resources.
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http://dx.doi.org/10.1155/2022/4258577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356798PMC
August 2022

Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation.

Oxid Med Cell Longev 2022 23;2022:9306614. Epub 2022 Jul 23.

Department of Immunology, Department of Biotherapy, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.

As a previously discovered target of DNA damage, Na/H exchanger 1 (NHE1) plays a role in regulation of intracellular pH (pH) through the extrusion of intracellular proton (H) in exchange for extracellular sodium (Na). Its abnormal expression and dysfunction have been reported in solid tumor and hematopoietic malignancies. Here, we reported that suppression of NHE1 in BCR-ABL hematopoietic malignancies' K562 cells treated with Etoposide was manipulated by miR-19 and c-MYC. Inhibition of miR-19 or c-MYC enhanced the expression of NHE1 and sensitized K562 cells to Etoposide . The nude mouse transplantation model was also performed to confirm the enhanced sensitivity of K562 cells to Etoposide by inhibiting the miR-19 or c-MYC pathway. TCGA analysis conferred a negative correlation between miR-19 level and leukemia patients' survival. Thus, our results provided a potential management by which the c-MYC-miRNA 19 pathway might have a crucial impact on sensitizing K562 cells to Etoposide in the therapeutic approaches.
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http://dx.doi.org/10.1155/2022/9306614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338868PMC
August 2022

Assessment of chemo-mechanical impacts of CO sequestration on the caprock formation in Farnsworth oil field, Texas.

Sci Rep 2022 Jul 29;12(1):13023. Epub 2022 Jul 29.

New Mexico Institute of Mining and Technology, 801 Leroy Place, Socorro, NM, 87801, USA.

This study evaluates the chemo-mechanical influence of injected CO on the Morrow B sandstone reservoir and the upper Morrow shale caprock utilizing data from the inverted 5-spot pattern centered on Well 13-10A within the Farnsworth unit (FWU). This study also seeks to evaluate the integrity of the caprock and the long-term CO storage capability of the FWU. The inverted 5-spot pattern was extracted from the field-scale model and tuned with the available field observed data before the modeling work. Two coupled numerical simulation models were utilized to continue the study. First, a coupled hydro-geochemical model was constructed to simulate the dissolution and precipitation of formation minerals by modeling three intra-aqueous and six mineral reactions. In addition, a coupled hydro-geomechanical model was constructed and employed to study the effects of stress changes on the caprock's porosity, permeability, and ground displacement. The Mohr-Coulomb circle and failure envelope were used to determine caprock failure. In this work, the CO-WAG injection is followed by the historical field-observed strategy. During the forecasting period, a Water Alternating Gas (WAG) injection ratio of 1:3 was utilized with a baseline bottom-hole pressure constraint of 5500 psi for 20 years. A post-injection period of 1000 years was simulated to monitor the CO plume and its effects on the CO storage reservoir and caprock integrity. The simulation results indicated that the impacts of the geochemical reactions on the porosity of the caprock were insignificant as it experienced a decrease of about 0.0003% at the end of the 1000-year post-injection monitoring. On the other hand, the maximum stress-induced porosity change was about a 1.4% increase, resulting in about 4% in permeability change. It was estimated that about 3.3% of the sequestered CO in the formation interacted with the caprock. Despite these petrophysical property alterations and CO interactions in the caprock, the caprock still maintained its elastic properties and was determined to be far from its failure.
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http://dx.doi.org/10.1038/s41598-022-16990-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338033PMC
July 2022

Teaching Analysis for Visual Communication Design with the Perspective of Digital Technology.

Comput Math Methods Med 2022 19;2022:2411811. Epub 2022 Jul 19.

Tangshan Normal University, Tangshan 063000, China.

The turn of contemporary visual culture has led to the expansion of the connotation and scope of visual communication design (VCD) education, the generation of new artistic concepts and forms, and the great changes in the subject education system. VCD instruction now has an enhanced teaching environment and operational platform because of the rapid advancement of digital technology. Digital technology is expected to break through traditional learning methods in the future and will be more widely integrated into VCD courses. A topic that must be addressed and explored in the reform and growth of VCD education is how to build a fairer and more inclusive college art education subject system. Therefore, it is particularly important to design a complete VCD teaching evaluation system. In this paper, artificial intelligence technology is applied to the teaching quality evaluation (TQE) system, and a scientific and reliable TQE model is obtained. The main works of this paper are as follows: (1) analyze the background and significance of TQE research, and systematically expound the domestic and foreign research status of TQE, genetic algorithm, and neural network. (2) Using an adaptive mutation evolutionary method, this research builds a TQE system for the VCD course and produces a BPNN model. The adaptive mutation genetic algorithm's convergence speed is considerably faster than the regular genetic algorithms, the optimized neural network's performance is also superior, and the model has a faster convergence time and better prediction accuracy.
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http://dx.doi.org/10.1155/2022/2411811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325565PMC
August 2022

Rab11a-Regulated Exosomes Inhibit Bacterial Infection through the Activation of Antilipopolysaccharide Factors in Crustaceans.

J Immunol 2022 Jul 27. Epub 2022 Jul 27.

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China;

Exosomes, secreted by most cells, are critical antimicrobial immune factors in animals. Recent studies of certain key regulators of vesicular transport, the Rab GTPases, have linked Rab dysfunction to regulation of innate immune signaling. However, the relationship between exosomes and Rab GTPases, resulting in antimicrobial activity in vertebrates and invertebrates during pathogenic infection, has not been addressed. In this study, Rab11a was reported to have a protective effect on the survival rate of mud crabs after s challenge through the stimulation of exosome secretion and modulation of anti-LPS factor (ALF) expression. Furthermore, 14-3-3 was confirmed to be densely packaged in exosomes after s infection, which could recruit the MyD88 and TLR by binding the Toll/IL-1R domain to the plasma membrane, promoting the translocation of Dorsal from the cytoplasm into the nucleus, and thereby regulating ALFs expression in the hemocytes of mud crab in response to the bacterial infection. The findings therefore provide, to our knowledge, a novel mechanism that underlies the cross-talk between Rab11a-regulated exosome formation and ALFs expression in innate immune response in invertebrates, with a crustacean species, mud crab , as a model study.
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http://dx.doi.org/10.4049/jimmunol.2200094DOI Listing
July 2022

Assessment of the Effect of Selenium Supplementation on Production of Selected Cytokines in Women with Hashimoto's Thyroiditis.

Nutrients 2022 Jul 13;14(14). Epub 2022 Jul 13.

Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland.

The impact of selenium on the course of Hashimoto's thyroiditis (HT) was mainly assessed by monitoring the titer of antithyroid autoantibodies in most of the studies conducted hitherto. On the other hand, the imbalance in activity of T cells such as Th1, Th2, Th17, and Treg may be relevant in the pathogenesis of this disease. Hence, the assessment of changes in the secretion of cytokines by these cells during selenium supplementation in patients with HT seems to be an important issue and was the main goal of this study. A further aim was to search for correlations among these cytokines, as well as markers of thyroid function, selenium/iodine status in the body, and other biochemical parameters. The group of 29 women with newly diagnosed Hashimoto's thyroiditis was supplemented with selenium in a dose of 100 µg/day for 6 months. Immunological parameters: interferon γ, tumor necrosis factor α, chemokine CXCL10, interleukin 4, interleukin 1β, interleukin 17, transforming growth factor β, and C-reactive protein, as well as selenium status parameters were determined in serum twice, i.e., before and after supplementation. Selenium supplementation was associated with a change in the production of two cytokines: interferon γ and interleukin 1β, for which a decrease and an increase in concentration were observed, respectively. The partial least squares (PLS) model revealed the presence of many relevant correlations among analyzed parameters. The stage of HT development, degree of thyroid dysfunction, and selenium supplementation of diet are interdependent factors which shape the profile of some cytokines secreted by cells participating in the autoimmunity process.
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http://dx.doi.org/10.3390/nu14142869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317246PMC
July 2022

Serum Free Zinc Is Associated With Vaccination Response to SARS-CoV-2.

Front Immunol 2022 30;13:906551. Epub 2022 Jun 30.

Institute for Experimental Endocrinology, Berlin Institute of Health, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany.

Background: Zinc (Zn) is an essential trace element with high relevance for the immune system, and its deficiency is associated with elevated infection risk and severe disease course. The association of Zn status with the immune response to SARS-CoV-2 vaccination is unknown.

Methods: A cohort of adult health care workers (n=126) received two doses of BNT162B2, and provided up to four serum samples over a time course of 6 months. Total SARS-CoV-2 IgG and neutralizing antibody potency was determined, along with total as well as free Zn concentrations.

Results: The SARS-CoV-2 antibodies showed the expected rise in response to vaccination, and decreased toward the last sampling point, with highest levels measured three weeks after the second dose. Total serum Zn concentrations were relatively stable over time, and showed no significant association with SARS-CoV-2 antibodies. Baseline total serum Zn concentration and supplemental intake of Zn were both unrelated to the antibody response to SARS-CoV-2 vaccination. Time resolved analysis of free Zn indicated a similar dynamic as the humoral response. A positive correlation was observed between free Zn concentrations and both the induced antibodies and neutralizing antibody potency.

Conclusion: While the biomarkers of Zn status and supplemental Zn intake appeared unrelated to the humoral immune response to SARS-CoV-2 vaccination, the observed correlation of free Zn to the induced antibodies indicates a diagnostic value of this novel biomarker for the immune system.
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http://dx.doi.org/10.3389/fimmu.2022.906551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280661PMC
June 2022

UPR signaling molecule, ATF6 is a poor prognostic marker in gastric cancer.

Asian J Surg 2022 Jul 11. Epub 2022 Jul 11.

Medical College of Yangzhou University, Yangzhou, 225001, China; Department of General Surgery, Northern Jiangsu People's Hospital, Clinical Medical College, Yangzhou University, Institute of General Surgery Yangzhou University, Nantong Road No.98, Yangzhou, 225001, China; General Surgery Institute of Yangzhou, Yangzhou University, Yangzhou, 225001, China; Northern Jiangsu People's Hospital, Yangzhou, 225001, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.057DOI Listing
July 2022

ITCH facilitates proteasomal degradation of TXNIP in hypoxia- induced lung cancer cells.

Thorac Cancer 2022 Aug 10;13(15):2235-2247. Epub 2022 Jul 10.

Department of Medical Oncology, Jinling Hospital, Nanjing, China.

Background: Lung cancer (LC) is one of the most common cancers and a leading cause of cancer-related deaths worldwide. In many pathological conditions, particularly in the tumor microenvironment, cells and tissues frequently exist in a hypoxic state. Here, we evaluated Itchy E3 ubiquitin protein ligase (ITCH) expression in LC cells following hypoxia treatment.

Methods: LC cell lines were treated with hypoxic condition. Cell migration, invasion, inflammation, reactive oxygen species (ROS) production, and apoptosis of LC cells were determined by wound healing assay, Transwell invasive assay, ELISA, DCFH-DA staining, and flow cytometry, respectively. qPCR and WB were used to determine the expression of ITCH and TXNIP. Co-IP was performed to assess the interaction between ITCH and TXNIP.

Results: ITCH expression was downregulated in LC cells under hypoxic conditions. Next, LC cells were subjected to hypoxic conditions and changes in cell viability and metastasis were determined. Hypoxic conditions resulted in increased migration and invasion abilities of LC cells. Intracellular reactive oxygen species (ROS) production, inflammation, and apoptosis were also promoted by hypoxia. We found that ITCH overexpression led to the proteasomal degradation of thioredoxin-interacting protein (TXNIP), whereas the expression of the ITCH C830A mutant did not affect TXNIP levels in LC cells. The gain-of-function experiment demonstrated that migration, invasion, ROS generation, inflammation, and apoptosis of hypoxia-conditioned LC cells were ameliorated by ITCH overexpression, whereas the ITCH C830A mutant did not cause any changes in these phenotypes. Furthermore, the contribution of TXNIP knockdown and ITCH overexpression to the hypoxia-induced features in LC cells with ITCH C830A was found to be similar.

Conclusion: Our results suggest a novel mechanism underlying the changes in ITCH-mediated malignant phenotypes of hypoxia-conditioned LC cells via TXNIP.
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http://dx.doi.org/10.1111/1759-7714.14552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346185PMC
August 2022

Esterase-responsive and size-optimized prodrug nanoparticles for effective intracranial drug delivery and glioblastoma treatment.

Nanomedicine 2022 Jul 8;44:102581. Epub 2022 Jul 8.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China. Electronic address:

Glioblastoma multiforme (GBM) is the intracranial malignancy with the highest rates of morbidity and mortality. Chemotherapy is often ineffective against GBM due to the presence of the blood-brain barrier (BBB); however, the application of nanotechnology is expected to overcome this limitation. Poly(lactic-co-glycolic acid) (PLGA) is a degradable and nontoxic functional polymer with good biocompatibility that is widely used in the pharmaceutical industry. Previous studies have shown that the ability of PLGA nanoparticles (NPs) to penetrate the BBB is largely determined by their size; however, determination of the optimal PLGA NP size requires further research. Here, we report a tandutinib-based prodrug (proTan), which responds to the GBM microenvironment, that was combined with NPs to overcome the BBB. AMD3100-PLGA NPs loaded with proTan inhibited tumor growth and effectively prolonged the survival of tumor-bearing mice.
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http://dx.doi.org/10.1016/j.nano.2022.102581DOI Listing
July 2022

Recent Progress on Regulating Strategies for the Strengthening and Toughening of High-Strength Aluminum Alloys.

Materials (Basel) 2022 Jul 5;15(13). Epub 2022 Jul 5.

Hubei Key Laboratory of Advanced Technology for Automotive Components, Wuhan University of Technology, Wuhan 430070, China.

Due to their high strength, high toughness, and corrosion resistance, high-strength aluminum alloys have attracted great scientific and technological attention in the fields of aerospace, navigation, high-speed railways, and automobiles. However, the fracture toughness and impact toughness of high-strength aluminum alloys decrease when their strength increases. In order to solve the above contradiction, there are currently three main control strategies: adjusting the alloying elements, developing new heat treatment processes, and using different deformation methods. This paper first analyzes the existing problems in the preparation of high-strength aluminum alloys, summarizes the strengthening and toughening mechanisms in high-strength aluminum alloys, and analyzes the feasibility of matching high-strength aluminum alloys in strength and toughness. Then, this paper summarizes the research progress towards adjusting the technology of high-strength aluminum alloys based on theoretical analysis and experimental verification, including the adjustment of process parameters and the resulting mechanical properties, as well as new ideas for research on high-strength aluminum alloys. Finally, the main unsolved problems, challenges, and future research directions for the strengthening and toughening of high-strength aluminum alloys are systematically emphasized. It is expected that this work could provide feasible new ideas for the development of high-strength and high-toughness aluminum alloys with high reliability and long service life.
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http://dx.doi.org/10.3390/ma15134725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268124PMC
July 2022

Experimental and Simulation Study for the Influence of Thermal Pre-Deformation on Subsequent Aging Precipitation Kinetics of Al-Zn-Mg-Cu Alloy.

Materials (Basel) 2022 Jul 1;15(13). Epub 2022 Jul 1.

School of Automotive Engineering, Wuhan University of Technology, Wuhan 430070, China.

Deformation and heat treatment are important means to strengthen aluminum alloys. However, the influence mechanism of pre-strain on aging precipitation kinetics and its effect on mechanical properties are still not clear. In this work, uniaxial isothermal tensile tests with different strains and artificial aging treatments for Al-Zn-Mg-Cu alloys have been carried out. Then, a model describing the precipitates kinetic behavior has been developed to investigate the effect of thermal pre-strain on subsequent aging precipitation kinetics and peak aging microhardness based on the microstructure characterization by TEM, SAXS and XRD tests. In addition, the role of dislocations on the aging precipitation kinetics is also explored. The experimental results show that the peak aging microhardness of the Al-Zn-Mg-Cu alloy reveals a tendency to decrease and increase and then the peak aging time firstly decreases and then keeps almost constant with the increase in the strain. The calculations demonstrate that the precipitate average size almost remains unchanged, while the precipitate volume fraction decreases and then increases with the increase in strain, which is consistent with the change in peak aging microhardness. It also indicates that dislocations can promote precipitate nucleation and growth, while the actual effect depends on the dislocation density, which is closely dependent on the pre-deformation condition, especially for the precipitate nucleation. In particular, when the dislocation density after thermal pre-deformation is not enough, it will slightly inhibit precipitate nucleation but promote precipitate growth, which could shorten the peak aging time, with the peak aging strength being guaranteed.
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http://dx.doi.org/10.3390/ma15134634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267310PMC
July 2022

Induced aging, structural change, and adsorption behavior modifications of microplastics by microalgae.

Environ Int 2022 Jun 28;166:107382. Epub 2022 Jun 28.

CAS Key Laboratory of Urban Pollutant Conversion, Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

The effects of microalgal biofouling on microplastic (MP) may differ from bacterial biofouling. In this study, the influence of microalgae on MP surface alteration, structural change, and adsorption of organic micropollutants was evaluated. Virgin polyethylene (PE), polyvinyl chloride (PVC), and polyamide (PA) were each immersed in algal photobioreactor and river freshwater for 30 days to simulate algal and river microbe biofouling respectively. Consequently, their physicochemical changes and adsorption potential of a mixture of six bisphenol analogues (BPA, BPS, BPE, BPB, BPF, BPAF) and two parabens (propyl-paraben, benzyl-paraben) were investigated. Owing to the algal bioactive compounds, major microalgae-induced biofouling and more MP aging than the river microbe aging were observed through fractures, pits, cracks, and algal attachments. Intrusion of algal organic matter and scission of polymeric functional groups were revealed during microalgal immersion and the potential MP aging pathways were proposed. Algal biofouling considerably altered the intrinsic properties of the MPs, consequently the adsorption capacity of PE and PVC was enhanced by 3.04-6.72 and 2.14-8.72 times, respectively. Adsorption process onto algal-aged MPs was pH-dependent, endothermic, non-spontaneous, and favored by hydrogen bonds. Meanwhile, the amide group in PA structure was conducive to organic micropollutant adsorption, which was likely reduced by algal aging and the erosion of the N-H stretching. Moreover, higher adsorption capacities of organic micropollutants were shown by the algal-biofilm PE and PVC than virgin and river microbial biofilm MPs. This study discloses that, biofouling and aging of MPs by microalgae through their bioactive components would engender more incidences on MP properties, organic micropollutants adsorption with associated environmental and health hazards.
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http://dx.doi.org/10.1016/j.envint.2022.107382DOI Listing
June 2022

Suppressing singlet-triplet annihilation processes to achieve highly efficient deep-blue AIE-based OLEDs.

Mater Horiz 2022 Jul 5. Epub 2022 Jul 5.

Center for Aggregation-Induced Emission, Institute of Polymer Optoelectronic Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, People's Republic of China.

Aggregation-induced emission (AIE) materials are attractive for the fabrication of high efficiency organic light-emitting diodes (OLEDs) by harnessing "hot excitons" from the high-lying triplet exciton states (T, ≥ 2) and high photoluminescence (PL) quantum efficiency in solid films. However, the electroluminescence (EL) efficiency of most AIE-based OLEDs does not meet our expectation due to some unrevealed exciton loss processes. Herein, we further enhance the efficiency of blue AIE-based OLEDs, and find experimentally and theoretically that the serious exciton loss is caused by the quenching of radiative singlet excitons and long-lived triplet excitons [singlet-triplet annihilation (STA)]. In order to suppress the STA process, 1-(2,5-dimethyl-4-(1-pyrenyl)phenyl)pyrene (DMPPP) with triplet-triplet annihilation up-conversion was doped in two AIE emitters to reduce the triplet excitons on the lowest triplet excited state (T) of AIE molecules. It can be seen that the external quantum efficiency (EQE) of the resulting blue OLEDs was enhanced to 11.8% with CIE coordinates of (0.15, 0.07) and a negligible efficiency roll-off, realizing the efficiency breakthrough of deep-blue AIE-based OLEDs. This work establishes a physical insight in revealing the exciton loss processes and the fabrication of high-performance AIE-based OLEDs.
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http://dx.doi.org/10.1039/d2mh00627hDOI Listing
July 2022

Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD+CD+ NKT-Like Cells.

J Oncol 2022 23;2022:8724933. Epub 2022 Jun 23.

Department of Immunology and Biotherapy, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

CD+CD+ natural killer T (NKT)-like cells have an immune function of T cells and NK cells, which play an important role in antitumor and antiviral immune responses. This study aims to establish a CD+CD+ NKT-like cell model by simulating the memory NK effect induced by cytokines IL-12, IL-15, and IL-18 (IL-12/15/18) and explore the formation mechanism. Our study found that the IL-12/15/18 preactivated CD+CD+ NKT-like cells exhibited enhanced IFN- production in response to restimulation with IL-12/15/18 for 6h on day 7. The intrinsic potential of these trained cells was significantly improved, showing an increase in IFN-, TNF-, and cell proliferation potential. The IFN- release, granzyme B level, and proliferation ability significantly increased when stimulated by NK-cell-sensitive K562 tumor cells. Among these cytokines, the combination of IL-12/15/18 was particularly effective. After the preactivation of IL-12/15/18, some cell surface proteins related to function and differentiation, such as CD11b, CD62 L, NKp46, NKG2A, and CD127, showed an evident and consistent change trend. The CDK4/6 inhibitor can effectively weaken this effect, and the expression of cyclin D1, Rb protein phosphorylation, and E2F-1 decreased significantly. Our work revealed that cytokine IL-12/15/18 can induce CD+CD+ NKT-like cells to obtain enhanced training immunity, which was a memory-like phenomenon.
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http://dx.doi.org/10.1155/2022/8724933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246603PMC
June 2022

Comparison of Conventional Logistic Regression and Machine Learning Methods for Predicting Delayed Cerebral Ischemia After Aneurysmal Subarachnoid Hemorrhage: A Multicentric Observational Cohort Study.

Front Aging Neurosci 2022 17;14:857521. Epub 2022 Jun 17.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Timely and accurate prediction of delayed cerebral ischemia is critical for improving the prognosis of patients with aneurysmal subarachnoid hemorrhage. Machine learning (ML) algorithms are increasingly regarded as having a higher prediction power than conventional logistic regression (LR). This study aims to construct LR and ML models and compare their prediction power on delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH).

Methods: This was a multicenter, retrospective, observational cohort study that enrolled patients with aneurysmal subarachnoid hemorrhage from five hospitals in China. A total of 404 aSAH patients were prospectively enrolled. We randomly divided the patients into training ( = 303) and validation cohorts ( = 101) according to a ratio of 75-25%. One LR and six popular ML algorithms were used to construct models. The area under the receiver operating characteristic curve (AUC), accuracy, balanced accuracy, confusion matrix, sensitivity, specificity, calibration curve, and Hosmer-Lemeshow test were used to assess and compare the model performance. Finally, we calculated each feature of importance.

Results: A total of 112 (27.7%) patients developed DCI. Our results showed that conventional LR with an AUC value of 0.824 (95%CI: 0.73-0.91) in the validation cohort outperformed k-nearest neighbor, decision tree, support vector machine, and extreme gradient boosting model with the AUCs of 0.792 (95%CI: 0.68-0.9, = 0.46), 0.675 (95%CI: 0.56-0.79, < 0.01), 0.677 (95%CI: 0.57-0.77, < 0.01), and 0.78 (95%CI: 0.68-0.87, = 0.50). However, random forest (RF) and artificial neural network model with the same AUC (0.858, 95%CI: 0.78-0.93, = 0.26) were better than the LR. The accuracy and the balanced accuracy of the RF were 20.8% and 11% higher than the latter, and the RF also showed good calibration in the validation cohort (Hosmer-Lemeshow: = 0.203). We found that the CT value of subarachnoid hemorrhage, WBC count, neutrophil count, CT value of cerebral edema, and monocyte count were the five most important features for DCI prediction in the RF model. We then developed an online prediction tool (https://dynamic-nomogram.shinyapps.io/DynNomapp-DCI/) based on important features to calculate DCI risk precisely.

Conclusions: In this multicenter study, we found that several ML methods, particularly RF, outperformed conventional LR. Furthermore, an online prediction tool based on the RF model was developed to identify patients at high risk for DCI after SAH and facilitate timely interventions.

Clinical Trial Registration: http://www.chictr.org.cn, Unique identifier: ChiCTR2100044448.
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http://dx.doi.org/10.3389/fnagi.2022.857521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247265PMC
June 2022

The prognostic role of NKG2A expression for patients with chronic myeloid leukemia after treatment discontinuation.

Leuk Lymphoma 2022 Jun 25:1-11. Epub 2022 Jun 25.

Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.

This study aims to evaluate the possibility of tyrosine kinase inhibitors (TKIs) discontinuation in chronic myeloid leukemia (CML) patients who obtained sustained deep molecular response (DMR) and to explore the prognostic role of NK cells in treatment-free remission (TFR). Sixty CML patients who discontinued TKI treatment were enrolled, and we also investigated the immune profiles in 27 CML patients after TKI cessation. Of the 60 patients, the estimated TFR rate was 60.8% [95% CI: 49.5-74.8%] at 12 months. Patients who had longer TKI duration, major molecular response, and DMR maintenance time had a significantly higher TFR rate. And a higher percentage of NKG2ANK cells and NKG2ACD56CD16NK cells were independent prognostic factors of TFR in multivariate analysis. These results indicate the practicality of the cessation of TKIs and patients with stable NK cell counts accompanied by higher cytotoxicity and increased killing capacity are more inclined to get sustained treatment-free survival.
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http://dx.doi.org/10.1080/10428194.2022.2090549DOI Listing
June 2022

Selenium Status in Paediatric Patients with Neurodevelopmental Diseases.

Nutrients 2022 Jun 8;14(12). Epub 2022 Jun 8.

Institute for Experimental Endocrinology, Charité-Universitätsmedizin Berlin, 13353 Berlin, Germany.

Neurodevelopmental diseases are often associated with other comorbidities, especially inflammatory processes. The disease may affect the trace element (TE) status, which in turn may affect disease severity and progression. Selenium (Se) is an essential TE required for the biosynthesis of selenoproteins including the transporter selenoprotein P (SELENOP) and extracellular glutathione peroxidase (GPX3). SELENOP deficiency in transgenic mice resulted in a Se status-dependent phenotype characterized by impaired growth and disturbed neuronal development, with epileptic seizures on a Se-deficient diet. Therefore, we hypothesized that Se and SELENOP deficiencies may be prevalent in paediatric patients with a neurodevelopmental disease. In an exploratory cross-sectional study, serum samples from children with neurodevelopmental diseases ( = 147) were analysed for total serum Se, copper (Cu), and zinc (Zn) concentrations as well as for the TE biomarkers SELENOP, ceruloplasmin (CP), and GPX3 activity. Children with epilepsy displayed elevated Cu and Zn concentrations but no dysregulation of serum Se status. Significantly reduced SELENOP concentrations were found in association with intellectual disability (mean ± SD (standard deviation); 3.9 ± 0.9 mg/L vs. 4.4 ± 1.2 mg/L, = 0.015). A particularly low GPX3 activity (mean ± SD; 172.4 ± 36.5 vs. 192.6 ± 46.8 U/L, = 0.012) was observed in phacomatoses. Autoantibodies to SELENOP, known to impair Se transport, were not detected in any of the children. In conclusion, there was no general association between Se deficiency and epilepsy in this observational analysis, which does not exclude its relevance to individual cases. Sufficiently high SELENOP concentrations seem to be of relevance to the support of normal mental development. Decreased GPX3 activity in phacomatoses may be relevant to the characteristic skin lesions and merits further analysis. Longitudinal studies are needed to determine whether the observed differences are relevant to disease progression and whether correcting a diagnosed TE deficiency may confer health benefits to affected children.
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http://dx.doi.org/10.3390/nu14122375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227519PMC
June 2022

Effectiveness of Dietary Supplement for Skin Moisturizing in Healthy Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Front Nutr 2022 2;9:895192. Epub 2022 Jun 2.

Dermatology Department, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: The dietary supplement industry offers many oral cosmetics that purportedly assist in skin moisturization often with unclear evidence supporting efficacy and safety. To update the accessible proofs pertaining to the safety and effectiveness of oral dietary supplements to facilitate skin moisturizing via an all-around review and meta-analysis.

Methods: Three on-line databases [Pubmed, Embase, and Cochrane Library (CENTRAL)] were retrieved from January 2000 to November 2021. An overall 66 randomized controlled trials (RCTs) of skin care were recognized. Meta-analysis was performed for dietary supplements with four or more available research.

Results: Oral collagen or ceramide resulted in a statistically significant increase in skin hydration and a decrease in transepidermal water loss (TEWL) compared to placebo. No benefits regarding the improvement of skin conditions in terms of water content and TEWL were observed for lactic acid bacteria or Lactobacillus fermented foods. A statistically significant and positive effect on skin hydration was observed for both hyaluronan and procyanidin, with an unknown effect on TEWL due to insufficient RCTs. There was a non-significant improvement in the water content of stratum corneum for astaxanthin based on subgroup analyses. Among the dietary supplements trialed in ≤ 3 RCTs, the judgment regarding their effects on skin moisturizing was prevented by inconsistent conclusions as well as insufficient research. All food supplements were safe throughout the research (normally ≤ 24 weeks).

Conclusion: Oral dietary supplements, including collagen, ceramides, hyaluronan, and procyanidin, were proven to be effective for skin moisturization. At present, for skin moisturization, the proofs supporting the recommendation of other dietary supplements, such as lactic acid bacteria and astaxanthin, are insufficient.

Systematic Review Registration: http://www.crd.york.ac.uk/PROSPERO/ identifier CRD42021290818.
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http://dx.doi.org/10.3389/fnut.2022.895192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201759PMC
June 2022

Identification of the glutamine synthetase (GS) gene family in four wheat species and functional analysis of Ta4D.GSe in Arabidopsis thaliana.

Plant Mol Biol 2022 Jun 18. Epub 2022 Jun 18.

College of Agronomy, Qingdao Agricultural University, Qingdao, 266109, China.

Drought stress can negatively impact crop yield and quality. Improving wheat yields under drought stress is a major objective of agronomic research. Glutamine synthetase (GS) is a key enzyme of nitrogen metabolism that is critical to plant growth and development in abiotic stress response. However, to date, no systemic characterization of the GS genes has yet been conducted in wheat and its close relatives. We identified a total of 15 GS genes in Triticum aestivum (2n = 6x = 42; AABBDD), as well as 9 GS genes in Triticum dicoccoides (2n = 4x = 28; AABB), 6 in Aegilops tauschii (2n = 2x = 14; DD), and 5 in Triticum urartu (2n = 2x = 14; AA). The 35 GSs were further clustered into five lineages according to the phylogenetic tree. Synteny analysis revealed that the three subgenomes in bread wheat retained extensive synteny between bread wheat and its three relative species. We identified three up-regulated TaGSs (Ta4A.GSe, Ta4B.GSe, and Ta4D.GSe) from transcriptome data after drought and salt stress. Ta4D.GSe was subsequently used for further functional studies, and its subcellular localization were determined in Arabidopsis protoplasts. Its overexpression in Arabidopsis enhanced drought tolerance by increasing the ability of scavenging of reactive oxygen species (ROS) and osmotic adjustment. We identified GS gene family in four wheat species and performed comparative analyses of their relationships, chromosome locations, conserved motif, gene structure, and synteny. The subcellular localization of Ta4D.GSe was detected and its drought tolerance function was demonstrated. Taken together, these findings provide insight into the potential functional roles of the GS genes in abiotic stress tolerance. KEY MESSAGE: This report clearly shows detailed characterization of GS gene family in four wheat species and demonstrates that Ta4D.GSe plays an important role in enhancing drought tolerance by improving the scavenging of ROS and osmotic adjustment ability in Arabidopsis.
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http://dx.doi.org/10.1007/s11103-022-01287-4DOI Listing
June 2022

Effects of Blood Culture Aerobic/Anaerobic Bottle Collection Patterns from Both Sides of the Body on Positive Blood Culture Rate and Time-to-Positivity.

Infect Drug Resist 2022 10;15:2995-3004. Epub 2022 Jun 10.

The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Background: Although the principles for blood cultures (BCs) guidelines provide a recommendation for collection patterns, the complexity of clinical practice occasionally prompts clinicians to adopt non-standard collection patterns. Here, we investigate the influences of different BC collection patterns on detection of pathogens.

Methods: The BC collection patterns of 96 hospitals were surveyed online. And a retrospective study of BC data from a tertiary hospital was conducted.

Results: The results showed that 53.1% of hospitals adopted the recommended patterns. Among the 1439 episodes of true-positive BCs, 67.4% were found in both the left- and right-sided bottles; 58.2% were found in both aerobic and anaerobic bottles.

Conclusion: The present study suggested that the rate of standard collection patterns of blood culture was low and the non-standard collection patterns were associated with decreased detection of pathogens. Simultaneous collection of blood on the left and right sides was recommended as an effective pattern of BC collection.
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http://dx.doi.org/10.2147/IDR.S358675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197171PMC
June 2022

PGAM1 regulation of ASS1 contributes to the progression of breast cancer through the cAMP/AMPK/CEBPB pathway.

Mol Oncol 2022 Aug 27;16(15):2843-2860. Epub 2022 Jun 27.

Department of Immunology, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, China.

Phosphoglycerate mutase 1 (PGAM1) is a crucial glycolytic enzyme, and its expression status has been confirmed to be associated with tumor progression and metastasis. However, the precise role and other biological functions of PGAM1 remain unclear. Here, we report that PGAM1 expression is upregulated and related to poor prognosis in patients with breast cancer (BC). Functional experiments showed that knockdown of PGAM1 could suppress the proliferation, invasion, migration, and epithelial-mesenchymal transition of BC cells. Through RNA sequencing, we found that argininosuccinate synthase 1 (ASS1) expression was markedly upregulated in BC cells following PGAM1 knockdown, and it is required to suppress the malignant biological behavior of BC cells. Importantly, we demonstrated that PGAM1 negatively regulates ASS1 expression through the cAMP/AMPK/CEBPB axis. In vivo experiments further validated that PGAM1 promoted tumor growth in BC by altering ASS1 expression. Finally, immunohistochemical analysis showed that downregulated ASS1 levels were associated with PGAM1 expression and poor prognosis in patients with BC. Our study provides new insight into the regulatory mechanism of PGAM1-mediated BC progression that might shed new light on potential targets and combination therapeutic strategies for BC treatment.
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http://dx.doi.org/10.1002/1878-0261.13259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348593PMC
August 2022

SGLT2 inhibitor-empagliflozin treatment ameliorates diabetic retinopathy manifestations and exerts protective effects associated with augmenting branched chain amino acids catabolism and transportation in db/db mice.

Biomed Pharmacother 2022 Aug 4;152:113222. Epub 2022 Jun 4.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China; Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China. Electronic address:

Empagliflozin (EMPA) is the first sodium-glucose co-transporter 2 inhibitor to significantly reduce cardiovascular and kidney complications in type 2 diabetes mellitus. Given this, we speculate that EMPA may have the potential to intervene in diabetic retinopathy (DR), which is another diabetes-specific microvascular complication. Db/db mice were treated with EMPA for different periods to observe the retinas and related mechanisms. EMPA effectively balanced body weight and blood glucose levels, mitigated ocular edema and microaneurysm in db/db mice. EMPA significantly inhibited oxidative stress, apoptosis and recovered tight junction in diabetic retinas. MS/MS analyses showed that EMPA suppressed aberrant branched-chain amino acid (BCAAs) accumulation in db/db retinas, which led to the inhibition of the mammalian target of rapamycin activation, downregulation of inflammation, and angiogenic factors, including TNF-ɑ, IL-6, VCAM-1, and VEGF induced by diabetes. Furthermore, branched-chain α-keto acids (BCKAs), which are catabolites of BCAAs, were increased in diabetic retinas and decreased with EMPA application. Moreover, branched-chain ketoacid dehydrogenase kinase (BCKDK) was enhanced, BCKDHA and BCKDHB were decreased in diabetic retinas. This could be reversed by EMPA treatment, thus promoting BCAAs catabolism to decrease BCAAs and BCKAs accumulation in diabetic retinas. The high levels of BCAAs in the plasma and enhanced L-type amino acid transporter 1 (LAT1) were responsible for the high levels of BCAAs in diabetic retinas, which could be inhibited by EMPA. Overall, EMPA could ameliorate DR manifestations. The normalization of BCAAs catabolism and intake may play a role in this process. This study supports EMPA as a protective drug against DR.
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http://dx.doi.org/10.1016/j.biopha.2022.113222DOI Listing
August 2022

The impact of the opening of high-speed rail on corporate financing constraints.

PLoS One 2022 6;17(6):e0268994. Epub 2022 Jun 6.

School of Economics and Management, Beijing Jiaotong University, Beijing, China.

In this paper, the data of A-share non-financial listed companies from 2008 to 2019 are used to study the impact of the opening of high-speed railway on corporate financing constraints with the difference-in-differences model. The research results show that the opening of high-speed rail can effectively alleviate the financing constraints faced by enterprises. Through the analysis of its influence mechanism, it is found that the opening of high-speed rail can reduce the degree of information asymmetry and agency conflicts between enterprises and their stakeholders, and thereby ease corporate financing constraints. At the same time, the opening of the high-speed rail has a more significant effect on alleviating the financing constraints of non-state-controlled enterprises, technology-intensive enterprises, and enterprises in inland areas. This research is not only a supplement to the economic consequences related to the opening of the high-speed rail, but also a further expansion of the research on the factors affecting corporate financing constraints.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268994PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200357PMC
June 2022

Prognostic Significance of the Stage at Which an MRD-Negative Status Is Achieved for Patients With Multiple Myeloma Who Received ASCT.

Front Oncol 2022 18;12:776920. Epub 2022 May 18.

Department of Hematology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objective: To explore the prognostic significance of the stage at which a minimal residual disease (MRD)-negative status is achieved for patients with newly diagnosed multiple myeloma (NDMM) who received autologous hematopoietic stem cell transplantation (ASCT).

Cases And Methods: A retrospective analysis of 186 NDMM patients who received "induction therapy-ASCT-maintenance therapy" in our center and achieved an MRD-negative status was performed. Patients were divided into three groups, A (induction therapy), B (3 months after ASCT), and C (maintenance therapy), according to the stage at which an MRD-negative status was achieved.

Results: The median time to progression (TTP) of 186 patients was not reached; the median overall survival (OS) was 113.8 months. The median TTP of the patients in three groups was not reached (P=0.013), and the median OS of the patients in three groups was not reached, not reached, and 71.2 months, respectively (P=0.026). Among patients with standard-risk cytogenetics, the median TTP of those in all three groups was not reached (P=0.121), and the median OS of the patients in three groups was not reached, not reached, and 99.6 months, respectively (P=0.091). Among patients with high-risk cytogenetics, the median TTP of those in three groups was not reached, 53.9 months, and 35.8 months (P=0.060), and the median OS was not reached, 71.2 months, and 60.2 months, respectively (P=0.625). Among patients with R-ISS stage I-II, the median TTP of those in three groups was not reached (P=0.174), and the median OS of the patients in three groups was not reached, not reached, and 99.6 months, respectively (P=0.186). Among the 29 patients with R-ISS stage III, the median TTP of those in the 3 groups were unreached, unreached, and 35.1 months (P<0.001), and the median OS was unreached, unreached, and 48.5 months, respectively (P=0.020). In all enrolled patients, the stage of reaching MRD-negative was an independent prognostic factor for TTP, rather than a prognostic factor for OS. The stage of reaching MRD-negative in patients with R-ISS III was an independent prognostic factor for OS.

Conclusion: For the same patients who are MRD-negative, the prognoses of those who achieve an MRD-negative status at different groups are different. The stage at which an MRD-negative status is achieved can predict the prognosis of patients with R-ISS stage III.
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http://dx.doi.org/10.3389/fonc.2022.776920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157574PMC
May 2022

Tumor-Resident T Cells, Associated With Tertiary Lymphoid Structure Maturity, Improve Survival in Patients With Stage III Lung Adenocarcinoma.

Front Immunol 2022 19;13:877689. Epub 2022 May 19.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Tertiary lymphoid structure (TLS) and tumor-resident memory T cells (T) play crucial roles in the anti-tumor immune response, facilitating a good prognosis in patients with cancer. However, there have been no reports on the relationship between T and TLS maturity. In this study, we detected T and the maturity of TLS by immunofluorescence staining and analyzed the relationship between their distribution and proportion in patients with lung adenocarcinoma (LUAD). The proportion of T within TLSs was significantly higher than that outside and was positively correlated with the survival of patients. In addition, the proportions of CD4CD103 T and CD8CD103 T were significantly increased with the gradually maturation of TLSs. We divided the patients into three levels (grade 1, grade 2, and grade 3) according to the presence of increasing maturation of TLSs. The proportion of CD103 T in grade 3 patients was significantly higher than that in grade 1 and grade 2 patients, suggesting a close relationship between CD103 T and TLS maturity. Furthermore, positive prognosis was associated with grade 3 patients that exhibited CD103 phenotype.
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http://dx.doi.org/10.3389/fimmu.2022.877689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161276PMC
May 2022

BCAS3 accelerates glioblastoma tumorigenesis by restraining the P53/GADD45α signaling pathway.

Exp Cell Res 2022 Aug 1;417(2):113231. Epub 2022 Jun 1.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China; Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China. Electronic address:

As in many other cancers, highly malignant proliferation and disordered cell division play irreplaceable roles in the exceedingly easy recurrence and complex progression of glioblastoma multiforme (GBM); however, mechanistic studies of the numerous regulators involved in this process are still insufficiently thorough. The role of BCAS3 has been studied in other cancers, but its role in GBM is unclear. Here, our goal was to investigate the expression pattern of BCAS3 in GBM and its potential mechanism of action. Using TCGA database and human GBM samples, we found that BCAS3 expression was up-regulated in GBM, and its high expression predicted poor prognosis. To further investigate the relationship between BCAS3 and GBM characteristics, we up-regulated and down-regulated BCAS3 expression in GBM to detect its effect on cell proliferation and cell cycle. At the same time, we established U87 cells stably overexpressing BCAS3 and generated an intracranial xenograft model to investigate the Potential role of BCAS3 in vivo. Finally, based on in vitro cell experiments and in vivo GBM xenograft models, we observed that BCAS3 significantly regulates GBM cell proliferation and cell cycle and that this regulation is associated with p53/GADD45α Signaling pathway. Taken together, our findings suggest that BCAS3 is inextricably linked to the progression of GBM and that targeting BCAS3 may have therapeutic effects in GBM patients.
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http://dx.doi.org/10.1016/j.yexcr.2022.113231DOI Listing
August 2022

Mesh plug erosion into the small intestine after inguinal hernia repair: A case report.

World J Clin Cases 2022 Apr;10(12):3944-3950

Department of General Surgery, the Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China.

Background: Mesh plug (MP) erosion into the intra-abdominal organs is a rare but serious long-term complication after inguinal hernia repair (IHR), and may lead to aggravation of symptoms if not treated promptly. It is difficult to diagnose MP erosion as there are no obvious specific clinical manifestations, and surgery is often needed for confirmation. In recent years, with the increased understanding of postoperative complications, MP eroding into the intra-abdominal organs has been a cause for concern among surgeons.

Case Summary: A 50-year-old man was referred to the Department of General Surgery with the complaint of abdominal pain in the right lower quadrant for 2 d. He had a surgical history of right open IHR and partial thyroidectomy performed 20 years and 15 years ago, respectively. Computed tomography revealed a circinate high-density image with short segmental thickening of the ileum stuck to the abdominal wall, and no evidence of recurrent inguinal hernia. Laparoscopic abdominal exploration confirmed adhesion of the middle segmental portion of the ileal loop to the right inguinal abdominal wall; the rest of the small intestine was normal. Further exploration revealed migration of the polypropylene MP into the intraperitoneal cavity and formation of granulation tissue around the plug, which eroded the ileum. Partial resection of the ileum, including the MP and end-to-side anastomosis with an anastomat, was performed.

Conclusion: Surgeons should aim to improve their ability to predict patients at high risk for MP erosion after IHR.
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http://dx.doi.org/10.12998/wjcc.v10.i12.3944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100743PMC
April 2022

A Novel Karyoplasmic Ratio-Based Automatic Recognition Method for Identifying Glioma Circulating Tumor Cells.

Front Oncol 2022 13;12:893769. Epub 2022 May 13.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Detection of circulating tumor cells (CTCs) is a promising technology in tumor management; however, the slow development of CTC identification methods hinders their clinical utility. Moreover, CTC detection is currently challenging owing to major issues such as isolation and correct identification. To improve the identification efficiency of glioma CTCs, we developed a karyoplasmic ratio (KR)-based identification method and constructed an automatic recognition algorithm. We also intended to determine the correlation between high-KR CTC and patients' clinical characteristics.

Methods: CTCs were isolated from the peripheral blood samples of 68 glioma patients and analyzed using DNA-seq and immunofluorescence staining. Subsequently, the clinical information of both glioma patients and matched individuals was collected for analyses. ROC curve was performed to evaluate the efficiency of the KR-based identification method. Finally, CTC images were captured and used for developing a CTC recognition algorithm.

Results: KR was a better parameter than cell size for identifying glioma CTCs. We demonstrated that low CTC counts were independently associated with isocitrate dehydrogenase (IDH) mutations ( = 0.024) and 1p19q co-deletion status ( = 0.05), highlighting its utility in predicting oligodendroglioma (area under the curve = 0.770). The accuracy, sensitivity, and specificity of our algorithm were 93.4%, 81.0%, and 97.4%, respectively, whereas the precision and F1 score were 90.9% and 85.7%, respectively.

Conclusion: Our findings remarkably increased the efficiency of detecting glioma CTCs and revealed a correlation between CTC counts and patients' clinical characteristics. This will allow researchers to further investigate the clinical utility of CTCs. Moreover, our automatic recognition algorithm can maintain high precision in the CTC identification process, shorten the time and cost, and significantly reduce the burden on clinicians.
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http://dx.doi.org/10.3389/fonc.2022.893769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137408PMC
May 2022

Psychometric properties of the Chinese version of the trust between People and Automation Scale (TPAS) in Chinese adults.

Psicol Reflex Crit 2022 May 30;35(1):15. Epub 2022 May 30.

Department of Developmental and Behavioral Pediatrics, Pediatric Translational Medicine Institution, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Trust in automation plays a leading role in human-automation interaction. As there lack of scales measuring trust in automation in China, the purpose of this study was to adapt the trust between People and Automation Scale (TPAS) into Chinese and to demonstrate its psychometric properties among Chinese adults. A total of 310 Chinese adults were randomly selected as sample 1, and 508 Chinese adults as sample 2. Results of the item analysis revealed that each item had a good quality, and the exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) suggested that the two-factor model with 12 items was the best fitting model. In addition, the TPAS was positively correlated with Interpersonal Trust Scale (ITS), proving good evidence based on relations to other variables to support the TPAS. In sum, the study suggested that the Chinese version of the TPAS could be used as an effective tool to assess trust in automation in the Chinese context.
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http://dx.doi.org/10.1186/s41155-022-00219-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148861PMC
May 2022
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