Publications by authors named "Qian Lu"

704 Publications

Poly(carbazole-co-1,4-dimethoxybenzene): Synthesis, Electrochemiluminescence Performance, and Application in Detection of Fe.

Polymers (Basel) 2022 Jul 27;14(15). Epub 2022 Jul 27.

School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

In this study, four polycarbazole derivatives (PCMB-Ds) with different alkyl side chains were designed and synthesized via Wittig-Horner reaction. A novel solid-phase electrochemiluminescence (ECL) system was prepared by immobilizing PCMB-D on an indium tin oxide (ITO) electrode with polyvinylidene fluoride (PVDF) in the presence of tripropylamine (TPrA). It could be found that the increase in alkyl side chain length had little effect on the ECL signal of PCMB-D, while the increase in the degree of polymerization (DP) greatly enhanced the ECL signal. Furthermore, the P-3/ITO ECL sensor based on the polyoctylcarbazole derivative (P-3) with the best ECL performance was successfully constructed and detected Fe under the optimal experimental conditions. The ECL signal steadily diminished with the increased concentration of Fe because of the competition and complexation between Fe and P-3 under the condition of pH 7.4. This P-3/ITO platform could realize a highly sensitive and selective detection of Fe with a wide detection range (from 6 × 10 mol/L to 1 × 10 mol/L) and low detection limit of 2 × 10 mol/L, which could allow the detection of Fe in multiple scenarios, and would have a great application prospect.
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http://dx.doi.org/10.3390/polym14153045DOI Listing
July 2022

Effects of Market Incentives and Livelihood Dependence on Farmers' Multi-Stage Pesticide Application Behavior-A Case Study of Four Provinces in China.

Int J Environ Res Public Health 2022 Aug 1;19(15). Epub 2022 Aug 1.

College of Economics and Management, Northwest A&F University, Xianyang 712100, China.

Improvement in pesticide application and efficiency structure has long been recognized as having great significance in reducing pollution, ensuring food safety, and promoting green agricultural development. Based on theoretical analysis, using the survey data of 766 farmers in key tea areas in Shaanxi, Sichuan, Zhejiang, and Anhui provinces in China, the study empirically analyzes the influence of market incentives and livelihood dependence on farmers' multi-stage pesticide application behavior. More specifically, the study employed ordered probit analysis to craft its findings. The dependent variable of this study is the multi-stage pesticide application problem of farmers, and the core independent variables are market incentives and livelihood dependence, and the judgment is based on the core variable coefficients of the econometric model of farmers at each stage. The study found the following: (i) Market incentives significantly prompted some farmers to give up synthetic pesticide application and farmers tend to choose green pesticides in the type of pesticide application. (ii) Livelihood dependence meant that the proportion of tea income significantly prompts farmers to apply pesticides, and also creates a tendency for farmers to choose green and low-toxic pesticides in the type of pesticide application. The planting period tends to have a moderate impact on applying green and low-toxic pesticides. (iii) The interaction term of market incentives and the proportion of tea income has no significant impact on farmers' multi-stage pesticide application behavior. The interaction term of market incentives and planting years has impacted negatively on whether farmers apply pesticides, and has no significant impact on farmers' choice of pesticide application types, but makes farmers increase the amount of green and low-toxic pesticides. (iv) The education level of the household head significantly promotes farmers to choose green and low-toxic pesticides. Seemingly, the brand effect of pesticides significantly encourages farmers to choose green and low-toxic pesticides. In external support, technical training significantly encourages farmers to choose green and low-toxic pesticides. Furthermore, better infrastructure and local market conditions significantly encourage farmers to reduce the use of conventional pesticides.
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http://dx.doi.org/10.3390/ijerph19159431DOI Listing
August 2022

Fatostatin inhibits SREBP2-mediated cholesterol uptake via LDLR against selective estrogen receptor α modulator-induced hepatic lipid accumulation.

Chem Biol Interact 2022 Aug 6;365:110091. Epub 2022 Aug 6.

New Drug Screening Center, Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Estrogen receptor α (ERα) plays a key role in the adaptive response of liver metabolism to energy demands, especially in controlling lipid metabolism. Tamoxifen (TMX), a main drug for the treatment of ER-positive breast cancer in clinical, is a selective ER modulator (SERM). However, accordingly, the long-term use of TMX would lead to nonalcoholic fatty liver (NAFLD) in clinical, which had no definite treatment up to now. Fatostatin (Fato), an inhibitor of sterol-regulatory element binding protein 2 (SREBP2), was reported as a synergistic inhibitor of ER-positive breast cancer with TMX, but the hepatic lipid regulation of this combination is still unknown. Herein, we aimed to explore the effect and mechanism of Fato against TMX-induced NAFLD. The results identified that hepatic cholesterol content increase was the main reason for TMX-induced NAFLD. It was caused by the upregulation of circulating cholesterol uptake mediated by low density lipoprotein receptor (LDLR) in liver, which resulted from the activation of SREBP2. Meanwhile, Fato could inhibit activation of SREBP2-LDLR pathway, alleviating TMX-induced hepatic cholesterol accumulation. In summary, these results provided a new insight into the mechanism of TMX-induced NAFLD. Moreover, it supported the combination of Fato and TMX for the treatment of ER-positive breast cancer to reduce the adverse effect of TMX in clinical.
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http://dx.doi.org/10.1016/j.cbi.2022.110091DOI Listing
August 2022

Trans-arterial positive ICG staining-guided laparoscopic liver watershed resection for hepatocellular carcinoma.

Front Oncol 2022 22;12:966626. Epub 2022 Jul 22.

Center of Hepatobiliary Pancreatic Disease, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

Introduction: Anatomical liver resection is the optimal treatment for patients with resectable hepatocellular carcinoma (HCC). Laparoscopic Couinaud liver segment resection could be performed easily as liver segments could be stained by ultrasound-guided indocyanine green (ICG) injection into the corresponding segment portal vein. Several smaller liver anatomical units (liver watersheds) have been identified (such as S8v, S8d, S4a, and S4b). However, since portal veins of liver watersheds are too thin to be identified under ultrasound, the boundaries of these liver watersheds could not be stained intraoperatively, making laparoscopic resection of these liver watersheds demanding. Digital subtraction angiography (DSA) could identify arteries of liver watersheds with a diameter of less than 2 mm. Yet, its usage for liver watershed staining has not been explored so far.

Purpose: The aim of this study is to explore the possibility of positive liver watershed staining trans-arterial ICG injection under DSA examination for navigating laparoscopic watershed-oriented hepatic resection.

Methods: We describe, in a step-by-step approach, the application of trans-arterial ICG injection to stain aimed liver watershed during laparoscopic anatomical hepatectomy. The efficiency and safety of the technique are illustrated and discussed in comparison with the laparoscopic anatomical liver resection ultrasound-guided liver segment staining.

Results: Eight of 10 HCC patients received successful trans-arterial liver watershed staining. The success rate of the trans-artery staining approach was 80%, higher than that of the ultrasound-guided portal vein staining approach (60%). Longer surgical duration was found in patients who underwent the trans-artery staining approach (305.3 ± 23.2 min vs. 268.4 ± 34.7 min in patients who underwent the ultrasound-guided portal vein staining approach, = 0.004). No significant difference was found in major morbidity, reoperation rate, hospital stay duration, and 30-day and 90-day mortality between the 2 groups.

Conclusions: Trans-arterial ICG staining is safe and feasible for staining the aimed liver watershed, navigating watershed-oriented hepatic resection under fluorescence laparoscopy for surgeons.
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http://dx.doi.org/10.3389/fonc.2022.966626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354495PMC
July 2022

Construction of Circadian Clock Signature for Tumor Microenvironment in Predicting Survival for Cutaneous Melanoma.

Curr Pharm Des 2022 Aug 2. Epub 2022 Aug 2.

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China.

Objective: We explored circadian clock-related genes (CCRG) to establish a risk model and identify associations with the tumor immune microenvironment in cutaneous melanoma (CM).

Methods: Circadian clock genes were downloaded from Circadian Gene Database. To explore CM-related circadian clock genes, we combined multivariate cox regression associated with least absolute shrinkage and selection operator (LASSO) regression in the Cancer Genome Atlas (TCGA) and validated it in the GSE65904 dataset. Time-dependent receiver operating characteristic curve (ROC) and Kaplan-Meier analysis were calculated to determine a CCRG risk score model. In addition, the overall survival nomograms of clinicopathological factors and circadian clock-associated gene signatures. Additionally, we evaluated the connection between circadian clock-associated genes with immune checkpoint inhibitors and immune cell infiltration.

Results: Two circadian-clock-related signatures were established. The risk model included SEMA4D (p < 0.001, HR: 0.709, 95% CI: 0.581 to 0.867) and SOD-2 (p<=0.009, HR: 0.790, 95% CI: 0.663 to 0.944) in patients with TCGA melanoma. The risk model was based on two CCRGs enriched in base excision repair, glycosylphosphatidyl (GPI ), and one carbon of the folate pathway. The overall survival was lower in the high-risk group. In addition, the circadian-clock signature may be able to evaluate the immunotherapy response.

Conclusions: We developed and validated a circadian signature to characterize the clinical significance and tumor microenvironment of cutaneous melanoma, revealing that circadian rhythms may impact cutaneous melanoma.
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http://dx.doi.org/10.2174/1381612828666220802114517DOI Listing
August 2022

Association between body composition and survival in head and neck cancer patients undergoing radiotherapy.

Head Neck 2022 Sep;44(9):2046-2054

Division of Medical and Surgical Nursing, School of Nursing, Peking University, Beijing, China.

Background: Body composition may influence the prognosis of head and neck cancer (HNC) patients. To find out the most crucial factors in this relationship, we explored the association between body composition and survival.

Methods: In this prospective longitudinal study, HNC patients who underwent radiotherapy (RT) from March 2017 to December 2018 were recruited. The association between body composition and survival was analyzed using Cox proportional hazard regression.

Results: Final analysis included 316 patients, with a median follow-up of 34.4 months. Multivariable analysis revealed that weight loss 6 months before RT and body composition changes during RT did not affect the survival outcome. However, patients with low appendicular skeletal muscle mass index (ASMI) before RT exhibited poor overall survival (OS). ASMI before RT was an independent prognostic factor for OS.

Conclusions: Body composition loss was common during RT, and ASMI before RT independently influenced the survival outcomes of HNC patients.
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http://dx.doi.org/10.1002/hed.27065DOI Listing
September 2022

The Characteristics of Fear of Recurrence and the Effect of Cognitive-Behavioral Stress Management Intervention in Patients after Radiofrequency Ablation of Atrial Fibrillation.

Evid Based Complement Alternat Med 2022 20;2022:6916302. Epub 2022 Jul 20.

Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou 215031, China.

The aim of this study was to grasp the current situation of fear of recurrence in patients after radiofrequency ablation of atrial fibrillation and to explore the application effect of cognitive-behavioral stress management in patients with fear of recurrence after radiofrequency ablation of atrial fibrillation. From July 2020 to July 2021, 150 patients with fear of recurrence of atrial fibrillation after radiofrequency ablation in our hospital were divided into a control group and an intervention group, with 75 cases in each group. Both groups received conventional nursing methods, and the intervention group received 8 weeks of cognitive-behavioral stress management. Before the intervention and after the intervention, the general situation questionnaire, the Chinese version of the Fear Disease Progression Simplified Scale (FoP-Q-SF), the Perceived Stress Scale (CPSS), and the Positive Psychological Capital Questionnaire (PPQ) were used for evaluation. Before the intervention, there were no significant differences in general data, degree of fear of recurrence, stress perception, and psychological capital scores between the two groups ( > 0.05). After 8 weeks of cognitive-behavioral stress management intervention, the fear recurrence, the total score of stress perception, and the scores of each dimension in the intervention group were significantly lower than those in the control group, and the psychological capital score was significantly higher than that in the control group, with statistically significant differences ( < 0.05). Cognitive-behavioral stress management method can significantly reduce the fear of recurrence and stress level of patients after radiofrequency ablation of atrial fibrillation, and improve their psychological capital level.
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http://dx.doi.org/10.1155/2022/6916302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328943PMC
July 2022

A multimodal model fusing multiphase contrast-enhanced CT and clinical characteristics for predicting lymph node metastases of pancreatic cancer.

Phys Med Biol 2022 Jul 29. Epub 2022 Jul 29.

School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Medical Image Processing, Guangdong Province Engineering Laboratory for Medical Imaging and Diagnostic Technology, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou, Guangdong, 510515, CHINA.

Objective: To develop a multimodal model that combines multiphase contrast-enhanced computed tomography (CECT) imaging and clinical characteristics, including experts' experience, to preoperatively predict lymph node metastasis in pancreatic cancer patients.

Methods: We proposed a new classifier fusion strategy based on a new evidential reasoning rule (CFS-nER) by combining nomogram weights into a previous evidential reasoning rule-based classifier fusion strategy. Three kernelled support tensor machine-based classifiers with plain, arterial, and venous phases of CECT as the inputs, respectively, were constructed. They were then fused based on the CFS-nER to construct a fusion model of multiphase CECT. The clinical characteristics were analyzed by univariate and multivariable logistic regression to screen risk factors, which were used to construct correspondent risk factor-based classifiers. Finally, the fusion model of the three phases of CECT and each risk factor-based classifier were fused further to construct the multimodal model based on our CFS-nER, named MMM-nER. This study consisted of 186 patients diagnosed with pancreatic cancer from four clinical centers in China, 88 (47.31%) of whom had lymph node metastasis.

Results: The fusion model of the three phases of CECT performed better overall than single and two-phase fusion models; this implies that the three considered phases of CECT were supplementary and complemented one another. The MMM-nER further improved the predictive performance, which implies that our MMM-nER can complement the supplementary information between CECT and clinical characteristics. The MMM-nER had better predictive performance than based on previous classifier fusion strategies, which presents the advantage of our CFS-nER.

Conclusion: We proposed a new CFS-nER, based on which the fusion model of the three phases of CECT and MMM-nER were constructed and performed better than all compared methods. MMM-nER achieved an encouraging performance, implying that it can assist clinicians in noninvasively and preoperatively evaluating the lymph node status of pancreatic cancer.
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http://dx.doi.org/10.1088/1361-6560/ac858eDOI Listing
July 2022

Exploration of dose tapering strategies for methadone maintenance treatment based on relapse risks: A subpopulation treatment effect pattern plot (STEPP) analysis.

Drug Alcohol Depend 2022 Jul 21;238:109581. Epub 2022 Jul 21.

Department of Medical Statistics, Sun Yat-sen University, Guangzhou, Guangdong, PR China. Electronic address:

Background: Half of methadone maintenance treatment (MMT) participants experience a tapering phase, however, the guidelines vary from country to country and lack individualized strategies based on relapse risk. A detailed and individualized tapering strategy is needed in China. This study aims to explore dose tapering strategies for Chinese individuals with different relapse risks.

Methods: A retrospective study was conducted from 2006 to 2017 at nine MMT clinics in Guangdong, China, involving 549 participants. The end point was the first relapse within 12 months of the start of tapering. Relapse risks before tapering for each participant were determined from a Cox model.

Results: Out of 549 participants, 173 (31.5 %) relapsed within 12 months after tapering. Findings indicated that a taper dose of less than 5 mg/week is better than other taper doses. Subpopulation treatment effect pattern plot (STEPP) methodology revealed different tapering strategies benefit participants according to relapse risk before tapering. Overall, findings indicated that a less than 5 mg/week reduction in MMT dose is better than reductions of other amounts. For participants with a low relapse risk before tapering, a reduction of less than 2.5 mg/week in MMT dose is better than a 2.5-5 mg/week reduction.

Conclusions: A taper dose of less than 5 mg/week appears to be the best dose tapering strategy for Chinese participants. Furthermore, for participants with a low relapse risk, a more gradual taper dose (less than 2.5 mg/week) works better than 2.5-5 mg/week. This benefit was not seen in participants with a high relapse risk before tapering.
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http://dx.doi.org/10.1016/j.drugalcdep.2022.109581DOI Listing
July 2022

Psychometric Properties of the Fertility Intention Scale among Patients with Breast Cancer of Childbearing Age in Mainland China.

Asia Pac J Oncol Nurs 2022 Aug 3;9(8):100100. Epub 2022 Jun 3.

Division of Medical & Surgical Nursing, Peking University School of Nursing, Beijing, China.

Objective: There are no valid assessment instruments assessing fertility intention among breast cancer survivors in mainland China. Therefore, this study aims to examine the psychometric properties of the Taiwanese version of the Fertility Intention Scale (FIS) among female patients with breast cancer of childbearing age in mainland China.

Methods: Two hundred and sixty-four female patients with breast cancer of childbearing age were recruited from two tertiary hospitals in Tianjin and Baoding, of which 32 patients completed the survey twice. Confirmatory factor analysis was adopted to assess construct validity. Correlations between the Reproductive Concerns After Cancer scale and FIS scores were calculated using Spearman correlation for convergent validity. The known-group validity of the FIS was verified using Mann-Whitney U test to compare the FIS scores between patients with or without the intention to conceive. Moreover, reliabilities were examined using Cronbach's alpha and intra-class correlation coefficient.

Results: Confirmatory factor analysis showed a good model fit to previous factor structures (χ/df = 3.19, root mean square error of approximation = 0.091, comparative fitting index = 0.980, Tucker-Lewis index = 0.975), and no FIS item was dropped. The FIS scores were weak negatively correlated with the Reproductive Concerns After Cancer scale scores ( = -0.172, < 0.01). The convergent validity of FIS was not satisfactory. Differences were noted between patients with or without the intention to conceive (50.62 ± 6.35 vs. 45.98 ± 7.19, < 0.01). The FIS showed acceptable known-group validity. The internal consistency (' = 0.824) and the test-retest reliability ( = 0.863, < 0.01) of the FIS were also acceptable.

Conclusions: Overall, the FIS provides a comprehensive evaluation of the fertility intention among patients with breast cancer of childbearing age in mainland China. However, the convergent validity was not satisfactory; thus, further revision and validation may be required in the future.
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http://dx.doi.org/10.1016/j.apjon.2022.100100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289818PMC
August 2022

Temperature control and intermittent time-set protocol optimization for minimizing tissue carbonization in microwave ablation.

Int J Hyperthermia 2022 ;39(1):868-879

Department of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, China.

Purpose: The charring tissue formation in the ablated lesion during the microwave ablation (MWA) of tumors would induce various unwanted inflammatory responses. This paper aimed to deliver appropriate thermal dose for effective ablations while preventing tissue carbonization by optimizing the treatment protocol during MWA with the set combinations of temperature control and pulsed microwave energy delivery.

Material And Methods: The thermal phase transition of porcine liver tissues were recorded by differential scanning calorimetry (DSC) to determine the temperature threshold during microwave output control. MWA was performed by an in-house built system with the ease of microwave output parameter adjustment and real-time temperature monitoring. The effects of continuous and pulsed microwave deliveries as well as various intermittent time-set of MWA were evaluated by measuring the dimensions of the coagulation zone and the carbonization zone.

Results: The DSC scans demonstrated that the porcine liver tissues have been in a state of endothermic heat during the heating process, where the maximum absorbed heat occurred at the temperature of 105 °C ± 5 °C. The temperature control during MWA resulted in effective coagulative necrosis while preventing tissue carbonization, after setting 100 °C as the upper threshold temperature and 60 °C as the lower threshold. Both the numerical simulation and experiments have shown that, upon the optimization of the time-set parameters in the periodic intermittent pulsed microwave output, the tissue carbonization was significantly diminished.

Conclusion: This study developed a straight-forward anti-carbonization strategy in MWA by modulating the pulsing mode and intermittent time. The programmed protocols of intermittent pulsing MWA have demonstrated its potentials toward future expansion of MWA technology in clinical application.
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http://dx.doi.org/10.1080/02656736.2022.2075041DOI Listing
July 2022

Genome-wide identification of drought-responsive microRNAs and their target genes in Chinese jujube by deep sequencing.

Genes Genomics 2022 Jul 12. Epub 2022 Jul 12.

Gansu Academy of Forestry Sciences, Lanzhou, 730020, People's Republic of China.

Background: MicroRNAs (miRNAs) are about 21 snucleotide (nt) long, non-coding RNAs that play an important role in plant abiotic stress responses. Chinese jujube is a native fruit tree in China, which is also an admittedly drought-resistant plant. But the drought-related miRNAs have little been reported in jujube.

Objective: To identify possibly drought-responsive microRNAs and their target genes in Chinese Jujube.

Methods: Twelve small RNA libraries were constructed from two jujube genotypes both drought treated and control samples with three replicates to identify known and novel miRNAs in Chinese Jujube, DESeq2 was used to identify expression pattern of miRNAs between drought treatment and control samples, TargetFinder program was used to predict potential target genes of conserved and novel miRNAs, RT-qPCR were used to analysis the expression levels of drought-related miRNAs and their potential targets. The RNA ligase-mediated RLM-5' RACE experiments were performed to validate predicted target genes of drought-related miRNAs.

Results: 43 known miRNAs and 431 novel miRNAs were identified in Chinese jujube. Expression analysis showed that 28 miRNAs were differential expressed under drought stress in jujube variety "Dongzao", including 21 up-regulated miRNAs and 7 down-regulated miRNAs, 61 miRNAs were differential expressed under drought stress in Chinese jujube variety "Zanhuangdazao", including 23 up-regulated miRNAs and 37 down-regulated miRNAs. Depend on miRNAs target prediction, functional annotation and expression analysis, we identified 9 drought-related miRNAs, and 7 target genes of 6 miRNAs were confirmed using the modified 5'-RACE method. Also, RT-qPCR analyses revealed that relative expression of those miRNAs and their targets have negative tendency.

Conclusion: We identified 6 drought-related miRNAs by high-throughout sequencing and target gene annotation from Chinese jujube, and targets of those miRNAs were confirmed by the modified 5'-RACE method. These findings provide molecular evidence for enhancing drought tolerance in Chinese jujube and other plants.
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http://dx.doi.org/10.1007/s13258-022-01274-5DOI Listing
July 2022

Hesperetin attenuates UVA-induced photodamage in human dermal fibroblast cells.

J Cosmet Dermatol 2022 Jul 11. Epub 2022 Jul 11.

Department of dermatology, The first affiliated hospital of Nanjing Medical University, Nanjing, China.

Background: Ultraviolet A (UVA) radiation causes skin damage. Recently, natural compounds have become an interest to protect skin from UV-induced photodamages.

Methods: In this study, we investigated the protective effects of hesperetin, a citrus flavonoid, on UVA-induced oxidative stress, inflammation, apoptosis, and photoaging.

Results: Our results showed that hesperetin increased the cell viability, suppressed the intracellular ROS levels, and decreased the expression of MMPs including MMP-1 and MMP-3, pro-inflammatory cytokines including IL-6 and COX-2 in UVA-irradiated HDFs. Besides, hesperetin exerted an anti-apoptotic effect by increasing expression of anti-apoptotic protein Bcl-2 and decreasing expression of pro-apoptotic protein Bax. Moreover, these anti-photodamage effects were mediated by inhibition of ERK, p38/AP-1, and NF-κb/p65 phosphorylation.

Conclusion: Therefore, hesperetin may be useful in the prevention of UVA-induced skin damage.
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http://dx.doi.org/10.1111/jocd.15230DOI Listing
July 2022

Phosphorylcholine zwitterionic shell-detachable mixed micelles for enhanced cancerous cellular uptakes and increased DOX release.

J Mater Chem B 2022 Jul 27;10(29):5624-5632. Epub 2022 Jul 27.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, P. R. China.

To further enhance the cancerous cellular uptakes and increase the drug release of the drug loaded micelles, herein, we fabricated a series of mixed micelles with different mass ratios using two amphiphilic copolymers P(DMAEMA--MaPCL) and PCL-SS-PMPC. The mixed micelles showed a prolonged circulation time due to the zwitterionic shells in a physiological environment (pH 7.4). In addition, because of the protonation of tertiary amine groups in PDMAEMA and the breakage of the disulfide bond in PMPC-SS-PCL in a tumor microenvironment, the mixed micelles aggregated, which led to enhanced cancerous cellular penetration and increased DOX release. Moreover, cytotoxicity assay showed that the mixed micelles had good biocompatibility to L929, HeLa and MCF-7 cells, even at a concentration of up to 1 mg mL. Furthermore, enhanced antitumour activity and cellular uptake of HeLa and MCF-7 cells were detected after loading with DOX, which was determined by confocal laser scanning microscopy (CLSM) and flow cytometry (FC), especially for the [email protected] 3 micelles (20% mass ratio of the P(DMAEMA--MaPCL)). Therefore, the mixed strategy provides a simple and efficient ways to promote anticancer drug delivery.
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http://dx.doi.org/10.1039/d2tb01061eDOI Listing
July 2022

PCycDB: a comprehensive and accurate database for fast analysis of phosphorus cycling genes.

Microbiome 2022 07 4;10(1):101. Epub 2022 Jul 4.

Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, 510006, China.

Background: Phosphorus (P) is one of the most essential macronutrients on the planet, and microorganisms (including bacteria and archaea) play a key role in P cycling in all living things and ecosystems. However, our comprehensive understanding of key P cycling genes (PCGs) and microorganisms (PCMs) as well as their ecological functions remains elusive even with the rapid advancement of metagenome sequencing technologies. One of major challenges is a lack of a comprehensive and accurately annotated P cycling functional gene database.

Results: In this study, we constructed a well-curated P cycling database (PCycDB) covering 139 gene families and 10 P metabolic processes, including several previously ignored PCGs such as pafA encoding phosphate-insensitive phosphatase, ptxABCD (phosphite-related genes), and novel aepXVWPS genes for 2-aminoethylphosphonate transporters. We achieved an annotation accuracy, positive predictive value (PPV), sensitivity, specificity, and negative predictive value (NPV) of 99.8%, 96.1%, 99.9%, 99.8%, and 99.9%, respectively, for simulated gene datasets. Compared to other orthology databases, PCycDB is more accurate, more comprehensive, and faster to profile the PCGs. We used PCycDB to analyze P cycling microbial communities from representative natural and engineered environments and showed that PCycDB could apply to different environments.

Conclusions: We demonstrate that PCycDB is a powerful tool for advancing our understanding of microbially driven P cycling in the environment with high coverage, high accuracy, and rapid analysis of metagenome sequencing data. The PCycDB is available at https://github.com/ZengJiaxiong/Phosphorus-cycling-database . Video Abstract.
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http://dx.doi.org/10.1186/s40168-022-01292-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252087PMC
July 2022

An investigation of knowledge, attitude and practice towards simulation among clinical nursing teachers in China: A cross-sectional study.

Nurse Educ Pract 2022 Jun 28;63:103395. Epub 2022 Jun 28.

Division of Medical & Surgical Nursing, School of Nursing, Peking University, #38 Xueyuan Road, Haidian District, Beijing 100191, China. Electronic address:

Aim: This study aimed to investigate the knowledge, attitude and practice towards simulation among CNTs in China and analyze the influencing factors.

Background: In China, simulation is now increasingly used by Clinical Nursing Teachers (CNTs) who are mainly responsible for nurses' continuing education, novice nurses training and nursing students practice in clinical settings. Over the past 20 years, enrolment scale of nursing education was expanded greatly, and the number of registered nurses increased from 1.22 to 4.10 million in China. To contribute to nurses' or nursing students' professional development and successful transition to competent practitioners, CNTs are now being challenged to use simulation to develop more learner-centered educational programs, which could better facilitate learner engagement. As faculty preparation is a prerequisite for conducting quality simulation, it is critical that CNTs have the requisite knowledge and skills to use simulation to its full potential. However, data on CNTs' knowledge, attitude and practice towards simulation is limited.

Design: A cross-sectional study.

Methods: A total of 342 CNTs from six tertiary affiliated teaching hospitals of a medical university in Beijing, China, participated in the study. Electronic questionnaire of Nurse Educators' Knowledge, Attitude and Practice towards Simulation (NEKAPS) was delivered to CNTs. Analysis of variance, t-test were used to analyze data.

Results: The mean score of knowledge and attitude for all participants was 60.01 ± 13.42 and 81.40 ± 19.99, respectively. The mean practice score was 70.65 ± 22.05 for 162 participants (47.4 %) who had conducted simulation in their hospitals. Participants with higher educational level (F=3.476, p = 0.032) and nursing career length≤ 15 years (t = 2.676, p = 0.008) had higher knowledge scores. However, there were no significant differences among different groups for attitudes. Participants with age ≤ 35 years old (t = 3.355, p = 0.001), nursing teaching length ≤ 10 years (t = 2.073, p = 0.041) and advanced beginner nurses (t = 3.212, p = 0.002) presented higher practice scores.

Conclusions: CNTs had a low level of knowledge on simulation. Their attitudes towards simulation were positive, whereas their practices were still lacking. It is highlighted an urgent need for standardized training of CNTs to improve their knowledge and practice towards simulation, so that they can optimize the simulation programs they provide for nurses or nursing students.
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http://dx.doi.org/10.1016/j.nepr.2022.103395DOI Listing
June 2022

TRIM33 drives prostate tumor growth by stabilizing androgen receptor from Skp2-mediated degradation.

EMBO Rep 2022 Aug 4;23(8):e53468. Epub 2022 Jul 4.

Cancer Centre, University of Macau, Taipa, Macau SAR.

Androgen receptor (AR) is a master transcription factor that drives prostate cancer (PCa) development and progression. Alterations in the expression or activity of AR coregulators significantly impact the outcome of the disease. Using a proteomics approach, we identified the tripartite motif-containing 33 (TRIM33) as a novel transcriptional coactivator of AR. We demonstrate that TRIM33 facilitates AR chromatin binding to directly regulate a transcription program that promotes PCa progression. TRIM33 further stabilizes AR by protecting it from Skp2-mediated ubiquitination and proteasomal degradation. We also show that TRIM33 is essential for PCa tumor growth by avoiding cell-cycle arrest and apoptosis, and TRIM33 knockdown sensitizes PCa cells to AR antagonists. In clinical analyses, we find TRIM33 upregulated in multiple PCa patient cohorts. Finally, we uncover an AR-TRIM33-coactivated gene signature highly expressed in PCa tumors and predict disease recurrence. Overall, our results reveal that TRIM33 is an oncogenic AR coactivator in PCa and a potential therapeutic target for PCa treatment.
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http://dx.doi.org/10.15252/embr.202153468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346471PMC
August 2022

Risk and main contributing factors for constipation in patients with gastrointestinal cancer: a multicenter cross-sectional study in China.

Support Care Cancer 2022 Jul 2. Epub 2022 Jul 2.

Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing), Nursing Department, Peking University Cancer Hospital & Institute, NO.52 Fucheng Road, Haidian District, Beijing, 100142, China.

Background And Objectives: The purpose of this study is to determine the risk of constipation and to identify the significant risk factors for constipation in patients with gastrointestinal cancer using the Chinese version of the constipation risk assessment scale (CRAS-C), as well as to explore the complementary constipation risk factors in patients with gastrointestinal cancer, to improve the specificity of the CRAS-C in this population, and finally to provide a theoretical basis for constipation prevention.

Research Design And Methods: A cross-sectional study involving multiple centers was conducted. A total of 190 patients with gastrointestinal cancer completed surveys that included demographic information, defecation habits, and the CRAS-C. The mean, SD, median, maximum, minimum, frequency, and percentage were used as indicators for the constipation risk and significant risk factors. The t test and Chi-square tests were used to analyze constipation indicators in patients with gastrointestinal cancer.

Results: The mean (SD) age of the 190 participants was 61.68 (12.35) years. The total CRAS-C mean (SD) score was 13.22 (4.69). Fifty-one patients (26.8%) scored lower than 11, who were at the low-risk level of constipation. One hundred thirty-nine patients (73.2%) scored 11 or above, who were at the high-risk level of constipation. The top 10 factors were insufficient liquid intake (81.1%), failure to consume bran products daily (78.9%), insufficient fiber intake (77.9%), antiemetics (74.7%), cytotoxic chemotherapy (52.6%), colorectal/abdominal diseases (42.6%), female (35.3%), opioid analgesics(26.8%), calcium channel blockers (16.3%), and endocrine disorders (14.2%). Chi-square test showed that constipating for most of the past 3 months, ascites and ECOG score were complementary constipation risk factors in gastrointestinal cancer patients.

Conclusion: The findings indicate that most gastrointestinal cancer patients were at a high risk of constipation. There are also several complementary constipation risk factors, and CRAS-C can be further revised in future studies to make it more specific in gastrointestinal cancer patients. Integrating CRAS into the pathway of constipation management, carrying out constipation risk screening for hospitalized cancer patients, and building systematic constipation prevention plan based on risk assessment are important to reduce the incidence of constipation in patients with gastrointestinal cancer and improve the quality of life.
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http://dx.doi.org/10.1007/s00520-022-07255-4DOI Listing
July 2022

Experiences of breast cancer survivors with lymphedema self-management: a systematic review of qualitative studies.

J Cancer Surviv 2022 Jun 30. Epub 2022 Jun 30.

Peking University School of Nursing, Beijing, China.

Purpose: This study systematically identified, evaluated, and synthesized qualitative literature on the experiences of breast cancer survivors with lymphedema self-management.

Methods: This systematic review followed the Joanna Briggs Institute meta-aggregation approach and was guided by the ENTREQ, graded according to the ConQual approach, and evaluated using the Joanna Briggs Institute Qualitative Assessment and Review Instrument (JBI-QARI). Qualitative studies related to the experiences in lymphedema self-management among breast cancer survivors conducted until March 9, 2022, were searched. The selected studies were reviewed independently, and the data were synthesized collaboratively into core themes.

Results: A total of 24 studies were included, and 85 findings resulted in three synthesis findings: (a) breast cancer survivors face challenges in lymphedema self-management, (b) breast cancer survivors are entangled in rebuilding a new balance between different roles, and (c) breast cancer survivors seek internal and external resources to regulate negative emotions.

Conclusions: Lymphedema self-management is a lifetime commitment and a challenge for breast cancer survivors, who find it difficult to adhere to self-management and cope with their problems. They require timely and continuous effective self-management education, and instrumental and emotional support from others, particularly healthcare providers and family members.

Implications For Cancer Survivors: Timely self-management education and access to lymphedema treatment and related resources are important for survivors to prevent and manage lymphedema. Breast cancer survivors should develop coping skills, and family members should participate in survivors' lymphedema self-management.
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http://dx.doi.org/10.1007/s11764-022-01225-9DOI Listing
June 2022

Cancer disparities in the context of rurality: risk factors and screening across various U.S. rural classification codes.

Cancer Causes Control 2022 Aug 30;33(8):1095-1105. Epub 2022 Jun 30.

Department of Obstetrics, Gynecology & Reproductive Biology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.

Purpose: Prior cancer research is limited by inconsistencies in defining rurality. The purpose of this study was to describe the prevalence of cancer risk factors and cancer screening behaviors across various county-based rural classification codes, including measures reflecting a continuum, to inform our understanding of cancer disparities according to the extent of rurality.

Methods: Using an ecological cross-sectional design, we examined differences in cancer risk factors and cancer screening behaviors from the Behavioral Risk Factor Surveillance System and National Health Interview Survey (2008-2013) across rural counties and between rural and urban counties using four rural-urban classification codes for counties and county-equivalents in 2013: U.S. Office of Management and Budget, National Center for Health Statistics, USDA Economic Research Service's rural-urban continuum codes, and Urban Influence Codes.

Results: Although a rural-to-urban gradient was not consistently evident across all classification codes, the prevalence of smoking, obesity, physical inactivity, and binge alcohol use increased (all p < 0.03), while colorectal, cervical and breast cancer screening decreased (all p < 0.001) with increasing rurality. Differences in the prevalence of risk factors and screening behaviors across rural areas were greater than differences between rural and urban counties for obesity (2.4% vs. 1.5%), physical activity (2.9% vs. 2.5%), binge alcohol use (3.4% vs. 0.4%), cervical cancer screening (6.8% vs. 4.0%), and colorectal cancer screening (4.4% vs. 3.8%).

Conclusions: Rural cancer disparities persist across multiple rural-urban classification codes, with marked variation in cancer risk factors and screening evident within rural regions. Focusing only on a rural-urban dichotomy may not sufficiently capture subpopulations of rural residents at greater risk for cancer and cancer-related mortality.
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http://dx.doi.org/10.1007/s10552-022-01599-2DOI Listing
August 2022

Effects of Comamonas testosteroni on dissipation of polycyclic aromatic hydrocarbons and the response of endogenous bacteria for soil bioremediation.

Environ Sci Pollut Res Int 2022 Jun 25. Epub 2022 Jun 25.

College of Life Sciences and Technology, Harbin Normal University, Harbin, 150025, China.

Bioremediation is a promising method of treating polycyclic aromatic hydrocarbons (PAHs) in contaminated soil; however, the understanding of the efficiency and the way of microbial inoculants work in complex soil environments is limited. Comamonas testosteroni (Ct) strains could efficiently degrade PAHs, especially naphthalene (Nap) and phenanthrene (Phe). This study aimed to explore the functional role of Ct in soil indigenous microorganisms and analyze the effect of Ct addition on PAHs concentration in PAH-contaminated soil. The results showed that inoculation with Ct degraded naphthalene (Nap), phenanthrene (Phe), and benzo [α] pyrene (BaP) significantly; the degradation rates were 63.38%, 81.18%, and 37.98% on day 25, respectively, suggesting that the low molecular weights of Nap and Phe were more easily degraded by microorganisms than those of BaP. We speculated that BaP and Phe might be converted into Nap for further degradation, which is the main reason for the low degradation rate of Nap detected after 10-25 days. Network analysis showed that inoculation with Ct significantly increased bacteria community abundance closely related to PAHs. Structural equation models confirmed that Steroidobacter, as functional bacteria, could affect the degradation of Nap and BaP. Inoculated Ct effectively enhanced the synergy among indigenous bacteria to degrade PAHs. This finding will help understand the function of inoculated Ct strains in PAH-contaminated soil at the laboratory level.
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http://dx.doi.org/10.1007/s11356-022-21497-zDOI Listing
June 2022

Cholesterol and matrisome pathways dysregulated in astrocytes and microglia.

Cell 2022 06;185(13):2213-2233.e25

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Nash Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Ronald M. Loeb Center for Alzheimer's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address:

The impact of apolipoprotein E ε4 (APOE4), the strongest genetic risk factor for Alzheimer's disease (AD), on human brain cellular function remains unclear. Here, we investigated the effects of APOE4 on brain cell types derived from population and isogenic human induced pluripotent stem cells, post-mortem brain, and APOE targeted replacement mice. Population and isogenic models demonstrate that APOE4 local haplotype, rather than a single risk allele, contributes to risk. Global transcriptomic analyses reveal human-specific, APOE4-driven lipid metabolic dysregulation in astrocytes and microglia. APOE4 enhances de novo cholesterol synthesis despite elevated intracellular cholesterol due to lysosomal cholesterol sequestration in astrocytes. Further, matrisome dysregulation is associated with upregulated chemotaxis, glial activation, and lipid biosynthesis in astrocytes co-cultured with neurons, which recapitulates altered astrocyte matrisome signaling in human brain. Thus, APOE4 initiates glia-specific cell and non-cell autonomous dysregulation that may contribute to increased AD risk.
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http://dx.doi.org/10.1016/j.cell.2022.05.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340815PMC
June 2022

Dual-Active [email protected] Nanozymes for Glucose Detection and Intracellular HO Modulation.

Langmuir 2022 Jul 22;38(26):8077-8086. Epub 2022 Jun 22.

China School of Chemical Engineering, Sichuan University, Chengdu 610065, P. R. China.

As a nanozyme, gold nanoparticles have some advantages compared with natural enzymes, such as stable structure, adjustable catalytic activity, multifunctionality, and recyclability. Due to their special dimension, they are easy to aggregate rapidly and lose their catalytic performance when exposed to normal saline or special pH environment. To avoid such a situation, [email protected] nanozymes with core-shell structure are constructed and their mimic peroxidase and glucose oxidase enzymatic activities are investigated. Kinetic examinations manifest that [email protected] nanozymes exhibited a high affinity for 3,3,5,5-tetramethylbenzidine (TMB), hydrogen peroxide (HO), and glucose. These predominant peroxidase-like and glucose-like oxidase [email protected] catalytic activities are successfully used in the detection of HO or glucose (LOD is 2.43 mM or 5.07 mM). Otherwise, the potential [email protected] nanozymes are provided with a clear ability for decomposing the intracellular HO in living cells. And it could protect cells from oxidative stress damage with inducing by HO. Therefore, it is easy to consider that [email protected] nanozymes show a certain possibility to retard cell senescence and increase the production of the hydroxyl radical which could prevent carcinogenesis of the cell.
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http://dx.doi.org/10.1021/acs.langmuir.2c00911DOI Listing
July 2022

Unexpected detection of a submillimeter early hepatocellular carcinoma focus by intraoperative near-infrared fluorescence imaging-a case report.

Ann Transl Med 2022 May;10(10):617

Nanjing Nuoyuan Medical Devices Co., Ltd., Nanjing, China.

Background: Prognosis of hepatocellular carcinoma (HCC) is closely related to residual tumor cells and tissues after tumor resection. Thus, close monitoring to ensure complete removal of residual tumor is fundamental. In this regard, intraoperative near-infrared fluorescence (NIRF) imaging has been of great assistance to surgeons for precision cancer surgeries. However, up to now, the identification of tiny lesions has not been reported. Herein, we report our findings on the case of an ultra-small HCC focus of about 430 µm that was successfully detected using NIRF during real-time monitored liver cancer surgery. The patient had a background of hepatitis B cirrhosis, which is the most phenomenon in China. Surgeons usually unable to distinguish sclerotic nodules from small tumor tissue with the naked eyes.

Case Description: A 55-year-old man with chronic hepatitis B infection was preoperatively diagnosed with a space-occupying liver lesion. A fluorescence signal was detected on the surface of the liver through the NIRF imaging system which had not been found by preoperative computed tomography (CT) and ultrasound examination. We subsequently tested the residual liver surface and observed a high signal point, less than 1 mm in the right anterior lobe of the liver. Histopathological examination revealed that the tiny fluorescent spot belong to an early HCC focus.

Conclusions: Based on these results, we think indocyanine green (ICG)-NIRF imaging may be used as a routine intraoperative detection method for liver cancer surgery in order to remove any residual tumor cells and tissue, hence minimizing further risk of remnant tumor regrowth.
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http://dx.doi.org/10.21037/atm-22-2338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201127PMC
May 2022

Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure.

J Transl Med 2022 06 11;20(1):267. Epub 2022 Jun 11.

Key Laboratory of Arrhythmias of the Ministry of Education of China, Research Center for Translational Medicine, Heart Failure Institute, Shanghai East Hospital, Jinzhou Medical University & Tongji University School of Medicine, 150 Jimo Road, Shanghai, 200120, China.

Heart failure (HF), as the leading cause of death, is continuing to increase along with the aging of the general population all over the world. Identification of diagnostic biomarkers for early detection of HF is considered as the most effective way to reduce the risk and mortality. Herein, we collected plasma samples from HF patients (n = 40) before and after medical therapy to determine the change of circulating miRNAs through a quantitative real-time PCR (QRT-PCR)-based miRNA screening analysis. miR-30a-5p and miR-654-5p were identified as the most significantly changed miRNAs in the plasma of patients upon treatment. In consistence, miR-30a-5p showed upregulation and miR-654-5p showed downregulation in the circulation of 30 HF patients, compared to 15 normal controls in the training phase, from which a two-circulating miRNA model was developed for HF diagnosis. Next, we performed the model validation using an independent cohort including 50 HF patients and 30 controls. As high as 98.75% of sensitivity and 95.00% of specificity were achieved. A comparison between the miRNA model and NT-pro BNP in diagnostic accuracy of HF indicated an upward trend of the miRNA model. Moreover, change of the two miRNAs was further verified in association with the therapeutic effect of HF patients, in which miR-30a-5p showed decrease while miR-654-5p showed increase in the plasma of patients after LVAD implantation. In conclusion, the current study not only identified circulating miR-654-5p for the first time as a novel biomarker of HF, but also developed a novel 2-circulating miRNA model with promising potentials for diagnosis and prognosis of HF patients, and in association with therapeutic effects as well.
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http://dx.doi.org/10.1186/s12967-022-03465-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188190PMC
June 2022

Better preparation is required for proactive advance care planning.

BMJ Support Palliat Care 2022 Jun 9. Epub 2022 Jun 9.

School of Nursing, Peking University, Beijing, China

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http://dx.doi.org/10.1136/bmjspcare-2022-003746DOI Listing
June 2022

Safety of inactivated COVID-19 vaccine in tuberous sclerosis complex patients with epilepsy treated with rapamycin.

Seizure 2022 Jul 16;99:71-74. Epub 2022 May 16.

Senior Department of Pediatrics, Chinese PLA General Hospital, Department of Pediatrics, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; Center for Brain Disorders Research, Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China. Electronic address:

Purpose: To assess the safety of inactivated coronavirus 2019 disease (COVID-19) vaccine in tuberous sclerosis complex (TSC) patients with epilepsy.

Methods: All patients with epilepsy were selected from Efficacy and Safety of Sirolimus in Pediatric Patients with Tuberous Sclerosis (ESOSPIT) project and younger than 17 years old. The patients were treated with mTOR inhibitors (rapamycin). A total of 44 patients who completed the two-dose inactivated COVID-19 vaccine between July 7, 2021, and January 1, 2022, were enrolled.

Results: The median age of seizure onset was 23 months. About two-thirds of patients have focal seizures. Thirty-three patients use antiseizure medications. The mean duration of rapamycin treatment was 55.59 ± 18.42 months. Adverse reactions within 28 days after injection occurred in 11 patients (25%), all were under 12 years old. Injection site pain was the most reported event (20.45%), which was mild in severity and improved within one day. All patients had no seizure-related changes after vaccination.

Conclusion: This study shows that the inactivated COVID-19 vaccine was well tolerated and safe in TSC patients with epilepsy, as well as for those treated with mTOR inhibitors.
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http://dx.doi.org/10.1016/j.seizure.2022.05.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110005PMC
July 2022

Observational Subseasonal Variability of the PM Concentration in the Beijing-Tianjin-Hebei Area during the January 2021 Sudden Stratospheric Warming.

Adv Atmos Sci 2022 16;39(10):1623-1636. Epub 2022 May 16.

Key Laboratory of Meteorological Disaster, Ministry of Education (KLME) / Joint International Research Laboratory of Climate and Environment Change (ILCEC) / Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters (CIC-FEMD), Nanjing University of Information Science and Technology, Nanjing, 210044 China.

It is still not well understood if subseasonal variability of the local PM in the Beijing-Tianjin-Hebei (BTH) region is affected by the stratospheric state. Using PM observations and the ERA5 reanalysis, the evolution of the air quality in BTH during the January 2021 sudden stratospheric warming (SSW) is explored. The subseasonal variability of the PM concentration after the SSW onset is evidently enhanced. Stratospheric circumpolar easterly anomalies lasted for 53 days during the January-February 2021 SSW with two evident stratospheric pulses arriving at the ground. During the tropospheric wave weakening period and the intermittent period of dormant stratospheric pulses, the East Asian winter monsoon weakened, anomalous temperature inversion developed in the lower troposphere, anomalous surface southerlies prevailed, atmospheric moisture increased, and the boundary layer top height lowered, all of which favor the accumulation of pollutant particulates, leading to two periods of pollution processes in the BTH region. In the phase of strengthened East Asian winter monsoon around the very beginning of the SSW and another two periods when stratospheric pulses had reached the near surface, opposite-signed circulation patterns and meteorological conditions were observed, which helped to dilute and diffuse air pollutants in the BTH region. As a result, the air quality was excellent during the two periods when the stratospheric pulse had reached the near surface. The increased subseasonal variation of the regional pollutant particulates after the SSW onset highlights the important role of the stratosphere in the regional environment and provides implications for the environmental prediction.
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http://dx.doi.org/10.1007/s00376-022-1393-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109736PMC
May 2022

Machine Learning for Prediction of Outcomes in Cardiogenic Shock.

Front Cardiovasc Med 2022 6;9:849688. Epub 2022 May 6.

Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

Objective: The management of cardiogenic shock (CS) in the elderly remains a major clinical challenge. Existing clinical prediction models have not performed well in assessing the prognosis of elderly patients with CS. This study aims to build a predictive model, which could better predict the 30-day mortality of elderly patients with CS.

Methods: We extracted data from the Medical Information Mart for Intensive Care III version 1.4 (MIMIC-III) as the training set and the data of validation sets were collected from the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University. Three models, including the cox regression model, the Least Absolute Shrinkage and Selection Operator (LASSO) regression model, and the CoxBoost model, were established using the training set. Through the comparison of area under the receiver operating characteristic (ROC) curve (AUC), C index, net reclassification improvement (NRI), integrated discrimination improvement (IDI), and median improvement in risk score, the best model was selected. Then for external validation, compared the best model with the simplified acute physiology score II (SAPSII) and the CardShock risk score.

Results: A total of 919 patients were included in the study, of which 804 patients were in the training set and 115 patients were in the verification set. Using the training set, we built three models: the cox regression model including 6 predictors, the LASSO regression model including 4 predictors, and the CoxBoost model including 16 predictors. Among them, the CoxBoost model had good discrimination [AUC: 0.730; C index: 0.6958 (0.6657, 0.7259)]. Compared with the CoxBoost model, the NRI, IDI, and median improvement in risk score of other models were all<0. In the validation set, the CoxBoost model was also well-discriminated [AUC: 0.770; C index: 0.7713 (0.6751, 0.8675)]. Compared with the CoxBoost model, the NRI, IDI, and median improvement in risk score of SAPS II and the CardShock risk score were all < 0. And we constructed a dynamic nomogram to visually display the model.

Conclusion: In conclusion, this study showed that in predicting the 30-day mortality of elderly CS patients, the CoxBoost model was superior to the Cox regression model, LASSO regression model, SAPS II, and the CardShock risk score.
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http://dx.doi.org/10.3389/fcvm.2022.849688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120613PMC
May 2022

Hepatic cortical blindness: a case report and comprehensive literature review.

Am J Med Sci 2022 May 16. Epub 2022 May 16.

Department of Gastroenterology, The Affiliated Hospital of Northwest University, Xi'an No.3 Hospital, Xi'an, Shaanxi, China.

Hepatic cortical blindness is an unusual clinical complication of hepatic encephalopathy, and its epidemiology has not been studied in detail. Herein, we present a case report of a 37-year-old male with liver cirrhosis and immunoglobulin A nephropathy who noted visual impairment after eating high-protein food. In addition to the case report, all previously documented cases of hepatic cortical blindness occurring across the globe published from inception until August 2019 were reviewed. The incidence of hepatic cortical blindness is low, and diagnoses are easily missed. The diagnosis of hepatic cortical blindness is dependent on symptoms, and patients may experience several attacks of vision loss. Most patients experience complete blindness, but vision loss recovers within 10 minutes to one month. Early detection and therapy for hepatic encephalopathy may improve patient outcome.
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http://dx.doi.org/10.1016/j.amjms.2022.04.034DOI Listing
May 2022
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