Publications by authors named "Qian Jin"

419 Publications

2D/1D BiOI/g-CN nanotubes heterostructure for photoelectrochemical overall water splitting.

Sci Total Environ 2022 May 23:156166. Epub 2022 May 23.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia.

To boost the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) performances, the BiOI/graphitic carbon nitride nanotubes (g-CN nanotubes) heterojunction was synthesized herein through the hydrothermal method. BiOI in-situ grew on the surface of g-CN nanotubes derived from melamine. The rapid recombination between photoexcited electrons and holes of pristine semiconductors was prevented via building the stable heterojunction. The SEM results indicated that the BiOI was wrapped around the surface of g-CN nanotubes, resulting in an optimized electronic transmission pathway. Much lower charge transfer resistance at the p-n heterojunction was demonstrated compared with pristine BiOI according to the EIS results, thus leading to the faster surface reaction rates. Moreover, the composite exhibited both outstanding OER and HER activities under illuminated conditions. This study may shed light upon establishing a bifunctional photoelectrocatalysis for photoelectrochemical water splitting based on stable 2D metal and 1D metal-free nanocomposite.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.156166DOI Listing
May 2022

Association of maternal obesity and gestational diabetes mellitus with overweight/obesity and fatty liver risk in offspring.

World J Gastroenterol 2022 Apr;28(16):1681-1691

Department of Gastroenterology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.

Background: Childhood obesity and fatty liver are associated with adverse outcomes such as diabetes, metabolic syndrome, and cardiovascular diseases in adulthood. It is very important to identify relevant risk factors and intervene as early as possible. At present, the relationship between maternal and offspring metabolic factors is conflicting.

Aim: To estimate the association of maternal obesity and gestational diabetes mellitus (GDM) with overweight/obesity and fatty liver risk in offspring at 8 years of age.

Methods: The prospective study included mothers who all had a 75-g oral glucose tolerance test at 24-28 wk of gestation and whose offspring completed follow-up at 8 years of age. Offspring birth weight, sex, height, weight, and body mass index (BMI) were measured and calculated. FibroScan-502 examination with an M probe (Echosens, Paris, France) was prospectively conducted in offspring aged 8 years from the Shanghai Prenatal Cohort Study.

Results: A total of 430 mother-child pairs were included in the analysis. A total of 62 (14.2%) mothers were classified as obese, and 48 (11.1%) were classified as having GDM. The mean age of the offspring at follow-up was 8 years old. Thirty-seven (8.6%) offspring were overweight, 14 (3.3%) had obesity, and 60 (14.0%) had fatty liver. The prevalence of overweight, obesity and fatty liver in offspring increased significantly across maternal BMI quartiles (all < 0.05). Among offspring of mothers with GDM, 12 (25.0%) were overweight, 4 (8.3%) were obese, and 12 (25.0%) had fatty liver vs. 25 (6.5%), 10 (2.6%) and 48 (12.6%), respectively, for offspring of mothers without GDM (all < 0.05). In multiple logistic regression, after adjustment for variables, the OR for fatty liver in offspring was 8.26 (95%CI: 2.38-28.75) for maternal obesity and GDM.

Conclusion: This study showed that maternal obesity can increase the odds of overweight/obesity and fatty liver in offspring, and GDM status also increases the odds of overweight/obesity in offspring. Weight management and glycemic control before and during pregnancy need to be highlighted in primary prevention of pediatric obesity and fatty liver.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3748/wjg.v28.i16.1681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048784PMC
April 2022

Exposure to nanoplastic induces cell damage and nitrogen inhibition of activated sludge: Evidence from bacterial individuals and groups.

Environ Pollut 2022 May 14;306:119471. Epub 2022 May 14.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China; College of Environment, Hohai University, Nanjing, 210098, People's Republic of China.

Wastewater treatment plants (WWTPs) are almost the only place where plastic fragments are artificially removed, resulting in mass accumulation of nanoplastics (NPs). In this research, four different concentrations (0 mg/L, 0.1 mg/L, 1 mg/L, 10 mg/L) of polystyrene nanoplastics (PS-NPs) were used to investigate the cell damage and nitrogen inhibition of activated sludge, exposed in a self-assembled SBR reactor for 30 days. Intracellular reactive oxides (ROS) and extracellular lactate dehydrogenase (LDH) increased with the rise of exposure concentration, and morphological analysis disclosed the creases, collapse, and even rupture of cell membranes. However, exposure damage (PS-NPs ≤ 1 mg/L) appeared to be reversible, attributed to that extracellular polymeric substances (EPS) secretion can thicken the three protective layers outside the membrane. PS-NPs did not disrupt the EPS chemical structure, but increased humic acid content. Prolonged exposure time (from 15 to 30 days) was directly related to the nitrogen inhibition. Due to the habitat changes under PS-NPs exposure, abundance and diversity of microorganisms in the original activated sludge decreased significantly, and the dominant phylum was occupied by Patescibacteria (PS-NPs = 10 mg/L). Changes in enzyme activities of AMO, NR, NIR, and NOR with exposure concentration may explain the conversion of nitrogen in SBR. This research broadens our horizons to understand the response mechanism of activated sludge bacteria to PS-NPs exposure individually and collectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2022.119471DOI Listing
May 2022

Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice.

Plant Physiol Biochem 2022 Jul 8;182:133-144. Epub 2022 Apr 8.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China. Electronic address:

Hydrogen sulfide (HS) improves aluminum (Al) resistance in rice, however, the underlying mechanism remains unclear. In the present study, treatment with 30-μM Al significantly inhibited rice root growth and increased the total Al content, apoplastic and cytoplasm Al concentration in the rice roots. However, pretreatment with NaHS (HS donor) reversed these negative effects. Pretreatment with NaHS significantly increased energy production under Al toxicity conditions, such as by increasing the content of ATP and nonstructural carbohydrates. In addition, NaHS stimulated the AsA-GSH cycle to decrease the peroxidation damage induced by Al toxicity. Pretreatment with NaHS significantly inhibited ethylene emissions in the rice and then inhibited pectin synthesis and increased the pectin methylation degree to reduce cell wall Al deposition. The phytohormones indole-3-acetic and brassinolide were also involved in the alleviation of Al toxicity by HS. The transcriptome results further confirmed that HS alleviates Al toxicity by increasing the pathways relating to material and energy metabolism, redox reactions, cell wall components, and signal transduction. These findings improve our understanding of how HS affects rice responses to Al toxicity, which will facilitate further studies on crop safety.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2022.04.006DOI Listing
July 2022

Effects of cluster nursing on cardiac function and quality of life in coronary heart disease patients with chronic heart failure: A protocol of randomized controlled trial.

Medicine (Baltimore) 2022 Apr 8;101(14):e29091. Epub 2022 Apr 8.

The First People's Hospital of Lianyungang, Department of Cardiovascular Medicine, No. 182 North Tongguan Road, Lianyungang, Jiangsu Province, P.R. China.

Background: Coronary heart disease (CHD) chronic heart failure has high morbidity and mortality, which poses a serious threat to patients' quality of life and life safety. For the treatment of chronic heart failure of CHD, in addition to drugs, high quality nursing measures are also very important. Cluster nursing is a high-quality nursing model based on evidence-based evidence. There is no clinical study to evaluate the effect of cluster nursing on cardiac function and quality of life of CHD patients with chronic heart failure.

Methods: This is a prospective randomized controlled trial to investigate the effects of cluster nursing on cardiac function and quality of life in patients with CHD chronic heart failure. Approved by the Clinical Research Ethics Committee of our hospital, patients will be randomly assigned to either routine nursing or cluster nursing. They will be followed up for 3 months after 4 weeks of treatment. Observation indicators include: The total effective rate of cardiac function improvement, Minnesota Living with Heart Failure Questionnaire, left ventricular ejection fraction, N-terminal pro-brain natriuretic peptide, 6-minute walk test, adverse reaction, etc. Data were analyzed using the statistical software package SPSS version 25.0.

Discussion: This study will evaluate the effects of cluster nursing on cardiac function and quality of life of CHD patients with chronic heart failure. The results of this study will provide clinical basis for establishing reasonable and effective nursing programs for CHD patients with chronic heart failure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000029091DOI Listing
April 2022

Light alters microbiota and electron transport: Evidence for enhanced mesophilic digestion of municipal sludge.

Water Res 2022 Jun 11;217:118447. Epub 2022 Apr 11.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, P. R. China; College of Environment, Hohai University, Nanjing 210098, P. R. China.

Light as an environmental factor can affect the process of anaerobic digestion, but there is no systematic study in municipal wastewater sludge mesophilic digestion. In this study, the effects of light on the performance of the anaerobic digestion system and photo-anaerobic microbiota (PAM) were evaluated in lighted anaerobic batch digesters (LABRs). The methane yield from the reactor under the dark condition (LABR0) was 179.2 mL CH/g COD, which was lower than 305.4 mL CH/g COD and 223.0 mL CH/g COD (n = 3, p < 0.05) from reactors under the light intensity of 3600 lm (LABR1) and 7200 lm (LABR2), respectively. The dominant genera in the bacterial and archaeal communities were Bacillus and Methanosarcina under light conditions, Enterococcus and Methanobacterium under dark conditions. And these two bacteria acted as electroactive bacterial genera, indicating that light changes the combination of direct interspecies electron transfer (DIET) microbial partners and activates the DIET pathway for methane production. The electron conduction pathways analysis further suggests that biological DIET (bDIET) between microbial biomass, rather than DIET via conductive material (cDIET) between microbes and conductive materials, is promoted and behaves as the dominant factor enhancing methane production under light conditions. The morphology of microorganisms and the amount and properties of EPS corroborate these views. Our findings are guided to anaerobic digester constructions under the outdoor environment with light exposure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2022.118447DOI Listing
June 2022

Surface Engineering of Cr-Doped Cobalt Molybdate toward High-Performance Hydrogen Evolution.

ACS Appl Mater Interfaces 2022 Apr 13;14(16):18607-18615. Epub 2022 Apr 13.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 201899, China.

Replacing commercial noble metal catalysts with earth-abundant metal catalysts for hydrogen production is an important research direction for electrolytic water. Improving the catalytic performance of non-noble metals while maintaining stability is a key challenge for alkaline hydrogen evolution. Herein, we combined alkali etching and surface phosphating to regulate the properties of Cr-doped CoMoO material, forming a surface structure in which amorphous cobalt phosphate and Cr-doped Co(Mo)O coexist. As expected, the as-prepared catalytic material exhibits remarkable hydrogen evolution activity in 1.0 M KOH, only requiring a low overpotential of 52.7 mV to achieve a current density of 10 mA cm, and can maintain this current density for 24 h. The characterization and analysis of the catalyst before and after the stability test reveal that the Cr doping and surface engineering (i.e., alkali etching and phosphating) synergistically increase the adsorption and dissociation of water, optimize the desorption of H, and ultimately accelerate hydrogen evolution. This work provides a new strategy for tailoring nonprecious metal materials to improve the hydrogen production from water electrolysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c03380DOI Listing
April 2022

Programming Soft Shape-Morphing Systems by Harnessing Strain Mismatch and Snap-Through Bistability: A Review.

Materials (Basel) 2022 Mar 24;15(7). Epub 2022 Mar 24.

Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province, Department of Engineering Mechanics, Zhejiang University, Hangzhou 310027, China.

Multi-modal and controllable shape-morphing constitutes the cornerstone of the functionalization of soft actuators/robots. Involving heterogeneity through material layout is a widely used strategy to generate internal mismatches in active morphing structures. Once triggered by external stimuli, the entire structure undergoes cooperative deformation by minimizing the potential energy. However, the intrinsic limitation of soft materials emerges when it comes to applications such as soft actuators or load-bearing structures that require fast response and large output force. Many researchers have explored the use of the structural principle of snap-through bistability as the morphing mechanisms. Bistable or multi-stable mechanical systems possess more than one local energy minimum and are capable of resting in any of these equilibrium states without external forces. The snap-through motion could overcome energy barriers to switch among these stable or metastable states with dramatically distinct geometries. Attributed to the energy storage and release mechanism, such snap-through transition is quite highly efficient, accompanied by fast response speed, large displacement magnitude, high manipulation strength, and moderate driving force. For example, the shape-morphing timescale of conventional hydrogel systems is usually tens of minutes, while the activation time of hydrogel actuators using the elastic snapping instability strategy can be reduced to below 1 s. By rationally embedding stimuli-responsive inclusions to offer the required trigger energy, various controllable snap-through actuations could be achieved. This review summarizes the current shape-morphing programming strategies based on mismatch strain induced by material heterogeneity, with emphasis on how to leverage snap-through bistability to broaden the applications of the shape-morphing structures in soft robotics and mechanical metamaterials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma15072397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999758PMC
March 2022

Synergistic promoting effect of increasing aquatic ammonium and CO on Microcystis aeruginosa.

Chemosphere 2022 Apr 8;301:134553. Epub 2022 Apr 8.

Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China; College of Environment, Hohai University, Nanjing, 210098, People's Republic of China.

Owing to climate change and intensive agricultural development, freshwater bodies have been affected by increases in both CO levels and chemically-reduced forms of N. However, little is known about how these changes affect cyanobacterial growth and blooms. This study explored a range of light conditions (30, 80, 130, or 200 μmol photons/m/s) wherein Microcystis aeruginosa, a widespread bloom-forming species, was exposed to different concentrations of CO (400 parts per million (ppm) and 1000 ppm) in a medium containing NH or NO-. The interactive effects of N sources and CO levels on the C/N metabolic balance and energy balance were examined to assess changes in the growth of M. aeruginosa. When the light intensity was 80 μmol photons/m/s, elevated CO could reduce intracellular reactive oxygen species (ROS) in NH-grown M. aeruginosa. Meanwhile, cell density and chlorophyll a (Chl a) increased with increasing CO levels, and the increase in Chl a was significantly greater in NH-grown M. aeruginosa than in NO--grown M. aeruginosa. Under light conditions of 200 μmol photons/m/s, elevated CO concentration caused NO--grown M. aeruginosa to be affected by a large amount of ROS, and the growth of NO--grown M. aeruginosa was finally suppressed. However, NH-grown M. aeruginosa had a smaller amount of ROS and showed improved growth as CO was elevated. This difference can be attributed to the faster metabolic pathways in the NH environment, which manifested in a lower accumulation of 2-oxoglutarate and fatty acids as CO was elevated. These findings suggest that the simultaneous increase in ammonium and CO in aquatic ecosystems confers cyanobacteria with greater advantages than the combination of nitrate and CO, which may aggravate cyanobacterial blooms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2022.134553DOI Listing
April 2022

Therapeutic application of chick early amniotic fluid: effective rescue of acute myocardial ischemic injury by intravenous administration.

Cell Regen 2022 Apr 1;11(1). Epub 2022 Apr 1.

Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China.

Myocardial regeneration has been considered a promising option for the treatment of adult myocardial injuries. Previously, a chick early amniotic fluid (ceAF) preparation was shown to contain growth-related factors that promoted embryonic growth and cellular proliferation, though the nature of the components within ceAF were not fully defined. Here we tested whether this ceAF preparation is similarly effective in the promotion of myocardial regeneration, which could provide an alternative therapeutic for intervening myocardial injury. In this study, a myocardial ischemic injury model was established in adult mice and pigs by multiple research entities, and we were able to show that ceAF can efficiently rescue damaged cardiac tissues and markedly improve cardiac function in both experimental models through intravenous administration. ceAF administration increased cell proliferation and improved angiogenesis, likely via down-regulation of Hippo-YAP signaling. Our data suggest that ceAF administration can effectively rescue ischemic heart injury, providing the key functional information for the further development of ceAF for use in attenuating myocardial injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13619-022-00110-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8975954PMC
April 2022

Identification of potential genes in endobronchial tuberculosis after bronchoscopic cryotherapy by transcriptome sequencing.

Infect Genet Evol 2022 06 15;100:105269. Epub 2022 Mar 15.

Endoscopy Clinic Center, Xi'an Chest Hospital, Xi'an 710100, Shaanxi, China. Electronic address:

Background: Bronchoscopic cryotherapy facilitates the treatment of endobronchial tuberculosis (EBTB) and helps suppress progressive bronchial stenosis. However, the molecular mechanism of bronchoscopic cryotherapy in EBTB patients has not been reported.

Methods: Transcriptome sequencing was performed to explore differentially expressed mRNAs (DEGs) in EBTB patients before and after bronchoscopic cryotherapy. Gene Ontology (GO) and KEGG analyses were carried out. Five genes (MKLN1, HIGD1A, PTGES, SKIL, and MCEMP1) were selected and validated using real-time qPCR (RT- qPCR).

Results: In transcriptome analysis, 448 DEGs with p < 0.05 and|logFC| > 1 were identified; of these, 171 and 277 DEGs were significantly up- and down-regulated after bronchoscopic cryotherapy, respectively. Results displayed 337 biological process (GO-BP), 48 cellular component (GO-CC) and 62 molecular function (GO-MF) terms and 20 KEGG pathways. RT- qPCR results were consistent with the sequencing data.

Conclusion: These data provide informative evidence and recommendations for further scientific research on bronchoscopic cryotherapy for EBTB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.meegid.2022.105269DOI Listing
June 2022

Blood Viscosity in Subjects With Type 2 Diabetes Mellitus: Roles of Hyperglycemia and Elevated Plasma Fibrinogen.

Front Physiol 2022 25;13:827428. Epub 2022 Feb 25.

Department of Engineering Mechanics, Zhejiang University, Hangzhou, China.

The viscosity of blood is an indicator in the understanding and treatment of disease. An elevated blood viscosity has been demonstrated in patients with Type 2 Diabetes Mellitus (T2DM), which might represent a risk factor for cardiovascular complications. However, the roles of glycated hemoglobin (HbA) and plasma fibrinogen levels on the elevated blood viscosity in subjects with T2DM at different chronic glycemic conditions are still not clear. Here, we evaluate the relationship between the blood viscosity and HbA as well as plasma fibrinogen levels in patients with T2DM. The experimental data show that the mean values of the T2DM blood viscosity are higher in groups with higher HbA levels, but the correlation between the T2DM blood viscosity and the HbA level is not obvious. Instead, when we investigate the influence of plasma fibrinogen level on the blood viscosity in T2DM subjects, we find that the T2DM blood viscosity is significantly and positively correlated with the plasma fibrinogen level. Further, to probe the combined effects of multiple factors (including the HbA and plasma fibrinogen levels) on the altered blood viscosity in T2DM, we regroup the experimental data based on the T2DM blood viscosity values at both the low and high shear rates, and our results suggest that the influence of the elevated HbA level on blood viscosity is quite limited, although it is an important indicator of glycemic control in T2DM patients. Instead, the elevated blood hematocrit, the enhanced red blood cell (RBC) aggregation induced by the increased plasma fibrinogen level, and the reduced RBC deformation play key roles in the determination of blood viscosity in T2DM. Together, these experimental results are helpful in identifying the key determinants for the altered T2DM blood viscosity, which can be used in future studies of the hemorheological disturbances of T2DM patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2022.827428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914209PMC
February 2022

Excellent Energy Storage Performance in Bi(FeMnTi)O Modified CaBiTiO Thin Film by Adjusting Annealing Temperature.

Nanomaterials (Basel) 2022 Feb 22;12(5). Epub 2022 Feb 22.

Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China.

Dielectric capacitors with ultrahigh power density are highly desired in modern electrical and electronic systems. However, their comprehensive performances still need to be further improved for application, such as recoverable energy storage density, efficiency and temperature stability. In this work, new lead-free bismuth layer-structured ferroelectric thin films of CaBiTiO-Bi(FeMnTi)O (CBTi-BFO) were prepared via chemical solution deposition. The CBTi-BFO film has a small crystallization temperature window and exhibits a polycrystalline bismuth layered structure with no secondary phases at annealing temperatures of 500-550 °C. The effects of annealing temperature on the energy storage performances of a series of thin films were investigated. The lower the annealing temperature of CBTi-BFO, the smaller the carrier concentration and the fewer defects, resulting in a higher intrinsic breakdown field strength of the corresponding film. Especially, the CBTi-BFO film annealed at 500 °C shows a high recoverable energy density of 82.8 J·cm and efficiency of 78.3%, which can be attributed to the very slim hysteresis loop and a relatively high electric breakdown strength. Meanwhile, the optimized CBTi-BFO film capacitor exhibits superior fatigue endurance after 10 charge-discharge cycles, a preeminent thermal stability up to 200 °C, and an outstanding frequency stability in the range of 500 Hz-20 kHz. All these excellent performances indicate that the CBTi-BFO film can be used in high energy density storage applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano12050730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911753PMC
February 2022

3D printing of tough hydrogels based on metal coordination with a two-step crosslinking strategy.

J Mater Chem B 2022 03 30;10(13):2126-2134. Epub 2022 Mar 30.

Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province, Department of Engineering Mechanics, Zhejiang University, Hangzhou 310027, China.

We demonstrate the self-supporting 3D printing of complex hydrogel structures based on simultaneous crosslinking reactions while printing. The printing strategy is based on the Schiff base reaction and metal coordination with a two-step crosslinking process. The printing ink was first prepared by dispersing oxidized sodium alginate (OSA) and adipic dihydrazide (ADH) in poly(acrylamide--acrylic acid) (P(AAm--AAc)) polymer solutions, and was mixed and printed into 3D structures with an extrusion-based coaxial printing platform. Because of the rapid chemical crosslinking reaction between the aldehyde group in OSA and the hydrazide group in ADH, the printed structures can be solidified quickly, and are further crosslinked by forming carboxyl-Fe coordination complexes to enhance their mechanical properties. The dynamic time-sweep rheological properties of the gel composed of different proportions of OSA and ADH were systematically investigated for the characteristic gelation time, and compression tests were carried out to measure the mechanical properties of the gel composed of OSA and ADH. Combining the gelation time and mechanical properties of the gel, the weight ratio of OSA and ADH was selected as 1 : 0.44 for an optimized setting in the subsequent printing. To evaluate the printability of inks, the material formula and printing parameters were systematically varied. The ink exhibited a wide printing range, self-supporting properties, and good printability. Tensile tests of the printed single fiber crosslinked by Fe show that its strength and toughness are tunable. Complex 3D structures such as pyramids, cylinders, and noses were constructed to demonstrate the printability of the ink. This printing method provides a facile approach for tough hydrogel fabrication without changing the rheological properties of the ink or sacrificing the ultimate mechanical properties of the printed materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1tb02529eDOI Listing
March 2022

A new treatment strategy for hemifacial microsomia: Auricular reconstruction with an expanded two-flap method and simultaneous mandibular distraction osteogenesis.

J Plast Reconstr Aesthet Surg 2022 Jan 31. Epub 2022 Jan 31.

Department of Maxillofacial Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Background: Owing to the complex clinical manifestations of hemifacial microsomia (HFM), multidisciplinary cooperation is required to achieve better therapeutic effects in terms of function and aesthetics. This study aimed to explore the efficacy of the expanded two-flap auricular reconstruction combined with mandibular distraction osteogenesis in the treatment of HFM.

Methods: This surgical strategy was performed in three stages. In the first stage, the retroauricular skin was expanded with a tissue expander and a mandibular distraction device was installed. In the second stage, the traditional expanded two-flap method for auricular reconstruction was adapted, and the framework was fabricated with costal cartilage and wrapped with the expanded skin flap, retroauricular fascia flap, and full-thickness skin graft. In the final stage, the tragus and lobule were rebuilt, the concha cavity deepened, and the mandibular distraction device removed.

Results: From January 2014 to November 2018, 166 HFM patients underwent auricular reconstruction with the expanded two-flap method and simultaneous mandibular extension in our hospital. The median follow-up period was 9.3 months. Of the 166 patients, 154 patients and their families were satisfied with the results, and only 16 patients experienced complications.

Conclusions: This three-stage technique of simultaneous auricular reconstruction and mandibular distraction osteogenesis is safe and effective in achieving facial symmetry, improving occlusal contact, shortening treatment course, and relieving patient's suffering, especially for HFM patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bjps.2022.01.035DOI Listing
January 2022

Prevalence and characteristics of MAFLD in Chinese adults aged 40 years or older: A community-based study.

Hepatobiliary Pancreat Dis Int 2022 Apr 31;21(2):154-161. Epub 2022 Jan 31.

Department of Gastroenterology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China. Electronic address:

Background: Nonalcoholic fatty liver disease (NAFLD) was recently proposed to be renamed metabolic dysfunction-associated fatty liver disease (MAFLD) with the diagnostic criteria revised. We investigated the similarities and differences in the prevalence and clinical characteristics of MAFLD and NAFLD in Chinese adults.

Methods: A cross-sectional study of 9980 Chinese individuals aged 40 years or older was performed between 2011 and 2012 using randomized, stratified cluster sampling in Shanghai, China. A detailed questionnaire and the results of abdominal ultrasonography, a standardized 2-h 75-g oral glucose tolerance test and blood biochemical examinations were collected.

Results: A total of 9927 subjects were included in this study. The prevalence of MAFLD (40.3%) was significantly higher than that of NAFLD (36.9%) (P < 0.05). MAFLD was highly prevalent in type 2 diabetes mellitus (T2DM) (53.8%), impaired fasting glucose (35.7%) and impaired glucose tolerance (40.9%). High risk of advanced fibrosis based on fibrosis-4 was highly prevalent (14.7%) in lean MAFLD with T2DM. Among 9927 subjects, 3481 (35.1%) fulfilled the diagnostic criteria for MAFLD and NAFLD (MAFLD+NAFLD+), 521 (5.2%) MAFLD+NAFLD-, and 181 (1.8%) MAFLD-NAFLD+. The MAFLD+NAFLD- group had more significant metabolic disorders than those in the MAFLD+NAFLD+ group (all P < 0.05). Among MAFLD-NAFLD+ subjects, 82.9% had metabolic disorders.

Conclusions: The new definition of MAFLD may better reflect the pathogenesis related to metabolism. Future research should focus on studying the natural history, pathogenesis and treatment effectivity of the overlap and non-overlap of NAFLD and MAFLD subjects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.hbpd.2022.01.006DOI Listing
April 2022

Radiosensitizing Effect of Celastrol by Inhibiting G2/M Phase Arrest Induced by the c-myc Gene of Human SW1353 Chondrosarcoma Cells: Network and Experimental Analyses.

Biomed Res Int 2022 31;2022:1948657. Epub 2022 Jan 31.

Department of Orthopedic Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.

Objective: Studies have unveiled that the components of Hook F (TWHF) such as celastrol could attenuate apoptosis and proliferation of various tumor cells. This study is focused on the radiosensitization effect and apoptotic pathways of celastrol via the inhibition of the gene and the influence of which combined with radiotherapy on the proliferation, apoptosis, invasion, and metastasis of chondrosarcoma cells.

Methods: A variety of bioinformatic tools were applied to explore the expression level and prognosis of the gene in different tumor cells and chondrosarcoma cells. We used pharmacology network to analyze the components, pathways, targets, molecular functions of TWHF and explore the relevant effective components over the MYC gene. Clone formation assay, CCK-8 assay, flow cytometry, and transwell migration assay were applied to detect the effects of celastrol on the expression of gene, cell apoptosis, and cell cycle. Radiation therapy was used to observe the radiosensitization effect of celastrol on chondrosarcoma.

Results: This study shows that the c-myc gene is overexpressed in various tumor cells and bone tumor cells to varying degrees. Celastrol can significantly inhibit the expression of the gene, induce G2/M phase arrest through regulation of G2/M phase-related proteins, and promote SW1353 cell apoptosis through the mitochondrial signaling pathway. In addition, we also found that the use of triptorubin to inhibit gene expression in combination with radiotherapy can increase the osteosarcoma cells' apoptosis rate through the mitochondrial signaling pathway significantly.

Conclusions: Our study validated the radiosensitization effect of celastrol through knocking down the expression of the c-myc gene to induce G2/M phase arrest and provides a new idea for the treatment of refractory or recurrent chondrosarcoma that is not sensitive to radiotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/1948657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820907PMC
March 2022

Transcriptome subtyping of metastatic Castration Resistance Prostate Cancer (mCRPC) for the precision therapeutics: an in silico analysis.

Prostate Cancer Prostatic Dis 2022 Jan 25. Epub 2022 Jan 25.

Departments of Urology, Huashan Hospital, Fudan University, No. 12 WuLuMuQi Middle Road, 200040, Shanghai, China.

Background: Men with metastatic prostate cancer who are treated with androgen deprivation therapy (ADT) typically develop therapeutic resistance eventually and have a dismal outcome. Moreover, the limited survival benefit observed in only a proportion of patients receiving novel therapeutics including immune checkpoint blockade and PARP inhibitors suggests the biological heterogeneity of mCRPCs.

Methods: To understand the heterogeneity of mCRPC, we analyzed the transcriptome of 231 mCRPCs and identified four disparate biological subtypes (Basal, Homologous Recombination Repair (HRR), Neuroendocrine and Luminal). The package "randomForest" was used to construct a Random Forest model. Circular plots were used for visualization of TMPRSS2-ERG fusion in each subtype.

Results: The Luminal subtype of mCRPCs has higher Androgen Receptor (AR) expression and copy number alterations as compared with the other subtypes. Genes in HRR pathway are relatively downregulated in most subtypes regardless of the genetic alterations, except for the HRR and NE subtypes, suggesting potential resistance of the HRR and NE mCRPCs to PARP inhibitor treatment. The HRR subtype has relatively more immune cell infiltration and higher expression of immune checkpoints, highlighting that the efficacy of immunotherapy should be evaluated in this particular subtype. TMPRSS2-ERG fusion is the most frequent gene fusion in all mCRPCs, and the Basal subtype has a higher frequency of this fusion than the other subtypes.

Conclusions: Our results reveal that the stratification of mCRPC according to transcriptome is informative of personalized therapeutics in the treatment of mCRPCs. The predictive capacity of the transcriptome subtyping of mCRPC warrants further exploration in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41391-022-00495-9DOI Listing
January 2022

An Analysis on Failure Reasons of Quit Smoking among Smokers in Beijing Communities-Based on a Cross-Sectional Study.

Biomed Environ Sci 2022 Jan;35(1):84-88

School of Public Health, Capital Medical University, Beijing 100069, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3967/bes2022.012DOI Listing
January 2022

Efficient basis sets for core-excited states motivated by Slater's rules.

Phys Chem Chem Phys 2022 Jan 26;24(4):2243-2250. Epub 2022 Jan 26.

Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

X-Ray photoemission spectroscopy is a commonly applied characterization technique that probes the local chemistry of atoms in molecules and materials the photoexcitation of electrons from atomic core orbitals. These measurements can be interpreted by comparison with previous literature or through the calculation of core-electron binding energies (CEBEs) for model systems. However, physically and numerically accurate description of the core-excited electronic structures demands specializations beyond routine ground state setups. Inspired by Slater's rules, we focus on developing computationally efficient and physically motivated contractions to reproduce the core-excited atomic orbitals which led to improved numerical accuracy of calculated CEBEs. When applied to carbon 1s excitations in a wide range of molecules, these core-excited basis sets produce total energy differences (ΔSCF) using a hybrid exact-exchange density functional (B3LYP) that can reproduce core-excitation energies within experimental accuracy (∼0.1 eV). Due to missing relativistic effects, heavier elements (N, O) exhibit slightly larger systematic absolute errors, but still maintain a satisfactory 0.2 eV mean average error for relative CEBEs. We also connect the known variability in the core level binding energy with local atomic charge to demonstrate how the transferability of a given model should be measured against a diverse test set. We conclude by exploring one outlier, CO, and the outlook for extending this approach to other elements.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cp03931hDOI Listing
January 2022

3D printing of a tough double-network hydrogel and its use as a scaffold to construct a tissue-like hydrogel composite.

J Mater Chem B 2022 01 19;10(3):468-476. Epub 2022 Jan 19.

Ministry of Education Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.

To mimic biological tissues with high toughness such as cartilage, it is highly desired to fabricate stable and tough hydrogels with intricate shapes to act as a structural support. Extrusion-based 3D printing is a promising method to fabricate 3D scaffolds with various architectures; however, printing tough hydrogel structures with high fidelity and resolution is still a challenge. In this work, we adopt the fast sol-to-gel transition of κ-carrageenan in the solution of acrylamide upon cooling to fix the printed scaffolds and polymerize the precursor solution to form the second network. The printed constructs of κ-carrageenan/polyacrylamide double-network gels are toughened by soaking in an aqueous solution of zirconyl chloride to form coordination complexes between the Zr ions and sulfate groups of κ-carrageenan. The obtained hydrogels are stable in water and possess good mechanical properties, with a tensile breaking stress of 1-2 MPa, breaking strain of 100-150%, and Young's modulus of 4-10 MPa. The printed grid can hold 150 times its own weight. 3D printed constructs with a high aspect ratio and shape fidelity are obtained by optimizing the printing parameters. Furthermore, a biomimetic strategy is applied to construct a hydrogel composite by filling the printed tough hydrogel scaffold with a cell-laden fibrin hydrogel as the soft substance. Chondrocytes in the hydrogel composite maintain high viability after cyclic compression, demonstrating the load-bearing capacity of the tough scaffold and favorable microenvironment for cells provided by the embedded soft fibrin gel. We envision that this printing strategy for hydrogel constructs with high toughness and good stability, as well as the method to form tough-soft hydrogel composites, can be extended to other systems to develop structural elements and scaffolds towards applications in biomedical devices and tissue engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1tb02465eDOI Listing
January 2022

5-aza-2'-deoxycytidine potentiates anti-tumor immunity in colorectal peritoneal metastasis by modulating ABC A9-mediated cholesterol accumulation in macrophages.

Theranostics 2022 1;12(2):875-890. Epub 2022 Jan 1.

Department of Biochemistry and Molecular Biology, Third Military Medical University (Army Medical University), Chongqing 400038, China.

5-aza-2'-deoxycytidine (5Aza), a DNA methyltransferase (DNMT) inhibitor, could activate tumor adaptive immunity to inhibit tumor progression. However, the molecular mechanisms by which 5Aza regulates tumor immune microenvironment are still not fully understood. The role of 5Aza in immune microenvironment of peritoneal carcinomatosis (PC) of colorectal cancer (CRC) was investigated. The effects of 5Aza on macrophage activation were studied by flow cytometry, real-time PCR, Western blotting assays, and Drug Affinity Responsive Target Stability (DARTS). The effects of 5Aza on tumor immunity were validated in stromal macrophages and T cells from CRC patients. 5Aza could stimulate the activation of macrophages toward an M1-like phenotype and subsequent activation of T cells in premetastatic fat tissues, and ultimately suppress CRC-PC in immune-competent mouse models. Mechanistically, 5Aza stimulated primary mouse macrophages toward to a M1-like phenotype characterized by the increase of p65 phosphorylation and IL-6 expression. Furthermore, we screened and identified ATP-binding cassette transporter A9 (ABC A9) as a binding target of 5Aza. 5Aza induced cholesterol accumulation, p65 phosphorylation and IL-6 expression in an ABC A9-dependent manner. Pharmacological inhibition of NF-κB, or genetic depletion of IL-6 abolished the antitumor effect of 5Aza in mice. In addition, the antitumor effect of 5Aza was synergistically potentiated by conventional chemotherapeutic drugs 5-Fu or OXP. Finally, we validated the reprogramming role of 5Aza in antitumor immunity in stromal macrophages and T cells from CRC patients. Taken together, our findings showed for the first time that 5Aza suppressed CRC-PC by regulating macrophage-dependent T cell activation in premetastatic microenvironment, meanwhile uncovered a DNA methylation-independent mechanism of 5Aza in regulating ABC A9-associated cholesterol metabolism and macrophage activation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.66420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692916PMC
March 2022

Erxian Decoction modulates Th17/Treg cells differentiation through LFA-1/ICAM-1/STAT3 pathway in menopausal dry eye disease.

Exp Eye Res 2022 02 22;215:108890. Epub 2021 Dec 22.

Department of Ophthalmology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 2011210, PR China. Electronic address:

With the development of modern societies and the ageing of the population, the treatment of menopausal dry eye disease (MDED) has become a thorny issue for the medical profession. Erxian Decoction (EXD) is a traditional Chinese medicine prescription, which has performed good clinical effect on dry eye disease. In this research, we purposed to investigate the molecular mechanisms of EXD for the treatment of MDED. A MDED rat model was established, the results indicated that high concentration of EXD could significantly improve the tear secretion and tear film stability of the animal model. Next, we found that EXD worked through the LFA-1/ICAM-1/STAT3 pathway in the body, and EXD could regulate IL-17, IL-10, CTLA-4 and TGF-β1 to get Th17/Treg balance. In vitro experiments, the results indicated that EXD affected the differentiation of CD4 T cells into Th17/Treg cells by inhibiting the expression and activation of LFA-1 on CD4 T cells, thus exerting immunotherapy effect. Our research provided the experimental basis and associated mechanisms for the clinical application of EXD in dry eye disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exer.2021.108890DOI Listing
February 2022

Effects of long-term perfluorooctane sulfonate (PFOS) exposure on activated sludge performance, composition, and its microbial community.

Environ Pollut 2022 Feb 15;295:118684. Epub 2021 Dec 15.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China; College of Environment, Hohai University, Nanjing, 210098, People's Republic of China.

The widespread presence and persistence of perfluorooctane sulfonate (PFOS) in wastewater treatment plants, as well as its toxicity and bioaccumulation potential, necessitates the investigation on their impact on bioreactor performance. A 48-day exposure test was adopted to study the effects of low (10 μg L-1) and high (1000 μg L-1) PFOS concentrations in a sequencing batch reactor on the performance, composition, and microbial community of activated sludge. The results suggested that adding PFOS at low and high concentrations lowered the removal efficiency of total nitrogen by 22.48% (p < 0.01) and 16.30% (p < 0.01) respectively, while enhanced that of total phosphorus by 1.87% (p > 0.05) and 7.07% (p < 0.05) respectively, compared with the control group. The addition of PFOS also led to the deterioration of activated sludge dewatering performance. Composition and spectroscopic measurements revealed that the PFOS dosage changed the composition of the activated sludge. Furthermore, the PFOS altered the structure and function of the activated sludge microbial community as well as key enzyme activities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.118684DOI Listing
February 2022

Inflammatory macrophages exploit unconventional pro-phagocytic integrins for phagocytosis and anti-tumor immunity.

Cell Rep 2021 12;37(11):110111

Laboratory of Molecular Oncology, Institut de recherches cliniques de Montréal (IRCM), Montréal, QC H2W 1R7, Canada; Department of Medicine, McGill University, Montréal, QC H3G 1Y6, Canada; Department of Medicine, University of Montréal, Montréal, QC H3C 3J7, Canada. Electronic address:

Blockade of the inhibitory checkpoint SIRPα-CD47 promotes phagocytosis of cancer cells by macrophages and is a promising avenue in anti-cancer therapy. Productive phagocytosis is strictly predicated on co-engagement of pro-phagocytic receptors-namely, Fc receptors (FcRs), integrin CD11b, or SLAMF7-by their ligands on cancer cells. Here, we examine whether additional pro-phagocytic receptors could be harnessed to broaden the scope of phagocytosis. Inflammatory stimuli, including multiple cytokines and Toll-like receptor (TLR) ligands, augment phagocytosis efficiency and fully alleviate the requirement of FcRs, CD11b, and SLAMF7 for phagocytosis. These effects are mediated by the unconventional pro-phagocytic integrins CD11a and CD11c, which act with CD18 to initiate actin polarization, leading to phagocytosis. Some inflammatory stimuli enable phagocytosis even in the absence of SIRPα-CD47 blockade. Higher CD11c expression in macrophage-enriched tumors correlates with improved survival in clinical studies. Thus, inflammatory macrophages exploit unconventional pro-phagocytic integrins for improved phagocytosis and anti-tumor immunity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2021.110111DOI Listing
December 2021

MicroRNA-133b Inhibition Restores EGFR Expression and Accelerates Diabetes-Impaired Wound Healing.

Oxid Med Cell Longev 2021 27;2021:9306760. Epub 2021 Nov 27.

Department of Orthopedics, Suizhou Hospital, Hubei University of Medicine, Suizhou 441300, China.

Diabetic foot ulcers (DFUs) are caused by impairments in peripheral blood vessel angiogenesis and represent a great clinical challenge. Although various innovative techniques and drugs have been developed for treating DFUs, therapeutic outcomes remain unsatisfactory. Using the GEO database, we obtained transcriptomic microarray data for DFUs and control wounds and detected a significant downregulation of epidermal growth factor receptor () in DFUs. We cultured human umbilical vein endothelial cells (HUVECs) and noted downregulated expression following high-glucose exposure . Further, we observed decreased HUVEC proliferation and migration and increased apoptosis after shRNA-mediated silencing in these cells. In mice, inhibition via focal EGFR-shRNA injection delayed wound healing. Target prediction analysis followed by dual-luciferase reporter assays indicated that microRNA-133b (miR-133b) is a putative upstream regulator of expression. Increased miR-133b expression was observed in both glucose-treated HUVECs and wounds from diabetes patients, but no such change was observed in controls. miR-133b suppression enhanced the proliferation and angiogenic potential of cultured HUVECs and also accelerated wound healing. Although angiogenesis is not the sole mechanism affected in DFU, these findings suggest that the miR-133b-induced downregulation of may contribute to delayed wound healing in diabetes. Hence, miR-133b inhibition may be a useful strategy for treating diabetic wounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9306760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643265PMC
February 2022

A Regulatory Network of Heat Shock Modules-Photosynthesis-Redox Systems in Response to Cold Stress Across a Latitudinal Gradient in Bermudagrass.

Front Plant Sci 2021 16;12:751901. Epub 2021 Nov 16.

College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Bermudagrass ( Pers.) is a wild turfgrass with various genotypes and phenotypes. In this study, 16 wild bermudagrass germplasms were collected from 16 different sites along latitudinal gradients, and different temperature treatments were compiled and used for physiological and transcriptome analysis. To explore the correlation between the key differentially expressed genes and physiological indicators, a total of 14,654 DEGs were integrated from the comparison of different temperature treatments and used for weighted gene co-expression network analysis. Through comparative transcriptome analysis and gene annotation, the results showed that differential gene expression profiles in networks are associated with the plant growth, photosystem, redox system, and transcriptional regulation to cold stress in bermudagrass. In particular, genes encoding HSP70/90 and HsfA3/A8 are not only regulated by temperature stress, but also directly or indirectly interplay with the processes of peroxide scavenging and chlorophyll synthesis under cold stress. Besides, through a weight evaluation analysis of various physiological indexes, we identified an accession of wild bermudagrass with relatively strong cold resistance. These results provide important clues and resources to further study the responses to low-temperature stress in bermudagrass.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.751901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636944PMC
November 2021

IL4/IL4R signaling promotes the osteolysis in metastatic bone of CRC through regulating the proliferation of osteoclast precursors.

Mol Med 2021 12 4;27(1):152. Epub 2021 Dec 4.

Department of Orthopedics, General Hospital of Western Theater Command, Rongdu Avenue No. 270, Chengdu, 610000, People's Republic of China.

Background: Bone metastasis of colorectal cancer (CRC) often indicates a poor prognosis. Osteolysis can be observed in metastatic sites, implying an aberrant activation of osteoclasts. However, how osteoclastogenesis is regulated in metastatic microenvironment caused by colorectal cancer is still unclear.

Methods: In this study, mice bone metastatic model of CRC was established through injection of MC-38 or CT-26 cells. BrdU assays showed primary CD115 ( +) osteoclast precursors (OCPs) proliferated at the first 2 weeks. Transcriptomic profiling was performed to identify differentially expressing genes and pathways in OCPs indirectly co-cultured with CRC cells RESULTS: The expression of IL4Rα was found to be significantly upregulated in OCPs stimulated by tumor conditioned medium (CM). Further investigation indicated that IL-4 signaling regulated proliferation of OPCs through interacting with type I IL4 receptor, and neutrophils were the main source of IL-4 in bone marrow. The proliferation of OCPs can be inhibited in IL4 deficiency mice. In addition, ERK pathway was activated by IL4/IL4R signaling. Ravoxertinib, an ERK antagonists, could significantly prevent bone destruction through inhibiting the proliferation of OCPs.

Conclusion: Our study indicates the essential role of IL4/IL4R signaling for the proliferation of OCPs in early metastasis of CRC predominantly through activating ERK pathway, which remarkedly impacts the number of osteoclasts in later stage and leads to osteolytic lesions. Moreover, Ravoxertinib could be a new therapeutical target for bone metastasis of CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s10020-021-00411-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642926PMC
December 2021

Population-attributable fractions of risk factors for all-cause dementia in China rural and urban areas: a cross-sectional study.

J Neurol 2022 Jun 28;269(6):3147-3158. Epub 2021 Nov 28.

Brain Science and Advanced Technology Institute, Wuhan University of Science and Technology, West Huangjiahu Road, Hongshan District, Wuhan, 430065, China.

Background: The prevalence of dementia in China, particularly in rural areas, is consistently increasing; however, research on population-attributable fractions (PAFs) of risk factors for dementia is scarce.

Methods: We conducted a cross-sectional survey, namely, the China Multicentre Dementia Survey (CMDS) in selected rural and urban areas from 2018 to 2020. We performed face-to-face interviews and neuropsychological and clinical assessments to reach a consensus on dementia diagnosis. Prevalence and weighted PAFs of eight modifiable risk factors (six classical: less childhood education, hearing impairment, depression, physical inactivity, diabetes, and social isolation, and two novels: olfactory decline and being unmarried) for all-cause dementia were estimated.

Results: Overall, CMDS included 17,589 respondents aged ≥ 65 years, 55.6% of whom were rural residents. The age- and sex-adjusted prevalence for all-cause dementia was 9.11% (95% CI 8.96-9.26), 5.19% (5.07-5.31), and 11.98% (11.8-12.15) in the whole, urban, and rural areas of China, respectively. Further, the overall weighted PAFs of the eight potentially modifiable risk factors were 53.72% (95% CI 52.73-54.71), 50.64% (49.4-51.89), and 56.54% (55.62-57.46) in the whole, urban, and rural areas of China, respectively. The eight risk factors' prevalence differed between rural and urban areas. Lower childhood education (PAF: 13.92%) and physical inactivity (16.99%) were primary risk factors in rural and urban areas, respectively.

Conclusions: The substantial urban-rural disparities in the prevalence of dementia and its risk factors exist, suggesting the requirement of resident-specific dementia-prevention strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00415-021-10886-yDOI Listing
June 2022

Flexible Lead-Free BaSrTiO/0.4BiFeO-0.6SrTiO Dielectric Film Capacitor with High Energy Storage Performance.

Nanomaterials (Basel) 2021 Nov 14;11(11). Epub 2021 Nov 14.

Institute for Superconducting and Electronic Materials, Australian Institute for Innovative Materials, University of Wollongong, Innovation Campus, North Wollongong, NSW 2500, Australia.

Ferroelectric thin film capacitors have triggered great interest in pulsed power systems because of their high-power density and ultrafast charge-discharge speed, but less attention has been paid to the realization of flexible capacitors for wearable electronics and power systems. In this work, a flexible BaSrTiO/0.4BiFeO-0.6SrTiO thin film capacitor is synthesized on mica substrate. It possesses an energy storage density of ~ 62 J cm, combined with an efficiency of ~ 74% due to the moderate breakdown strength (3000 kV cm) and the strong relaxor behavior. The energy storage performances for the film capacitor are also very stable over a broad temperature range (-50-200 °C) and frequency range (500 Hz-20 kHz). Moreover, the and are stabilized after 10 fatigue cycles. Additionally, the superior energy storage capability can be well maintained under a small bending radius ( = 2 mm), or after 10 mechanical bending cycles. These results reveal that the BaSrTiO/0.4BiFeO-0.6SrTiO film capacitors in this work have great potential for use in flexible microenergy storage systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11113065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622521PMC
November 2021
-->