Publications by authors named "Qian He"

484 Publications

induction and characterisation of tetraploid drumstick tree ( Lam.).

Open Life Sci 2020 18;15(1):840-847. Epub 2020 Nov 18.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangzhou, 510642, China.

Artificial induction of polyploidy is widely used in breeding programmes to improve the agronomic traits. The drumstick tree ( Lam.) has a range of potential commercial uses, as the vegetative organs have high nutritional, medicinal, and feed values. In the present study, tetraploidisation in drumstick tree was performed by treating leaf segments with colchicine and subsequently verifying the ploidy levels. For polyploidisation, explant survival and regeneration rates were affected more by exposure time than by colchicine concentration, and the highest polyploidisation efficiency was observed at 500 mg/L colchicine for 3 days, which yielded 21% tetraploids. The morphological characteristics and contents of seven fodder-related nutrients (crude protein, ether extract, ash, acid detergent fibre, neutral detergent fibre, calcium, and phosphorus) in the leaves and shoots were compared between tetraploid and diploid drumstick trees. The resulting tetraploids showed significantly enhanced leaf and stomatal size. In addition, the contents of seven fodder-related nutrients were higher, although to varying degrees, in tetraploids than in diploids. The results indicated that the tetraploid produced in this study exhibited superior agronomical traits and improved biomass yield than diploids, and may represent excellent raw materials for fodder to enhance biomass and nutrition.
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http://dx.doi.org/10.1515/biol-2020-0087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747515PMC
November 2020

Spectroscopic investigations of the changes in ligand conformation during the synthesis of soy protein-templated fluorescent gold nanoclusters.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 19;255:119725. Epub 2021 Mar 19.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Jiangsu 214122, China. Electronic address:

In this paper, the potential relationship between fluorescence and changes in the ligand conformation observed during the synthesis of soy protein-templated fluorescent gold nanoclusters (SP-AuNCs) was studied using a series of spectroscopic techniques. The results show that the determinants of the fluorescence effect in SP-AuNCs changed with the reaction time during the synthesis process. In the early stage of the reaction (within 60 min), the fluorescence intensity was dominated by the Au nucleus, followed by the combination of the Au nucleus and protein ligand. The structure of the protein ligand also underwent a transition from ordered to disordered to ordered. At the same time, its role in the reaction also changed from providing the reducing power to protecting the Au nucleus and contributing to the transition of the fluorescence effect in the AuNCs via ligand-to-metal charge transfer (LMCT). Using two-dimensional (2D) photon spectra correlation analysis, the formation and growth of the Au nuclei and the LMCT effect observed during the synthesis of the SP-AuNCs were found to be the major causes for the changes in the conformation of the protein ligand. Our results are an important discovery and can be used to explain the mechanism of protein ligands in the synthesis of gold nanoclusters.
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http://dx.doi.org/10.1016/j.saa.2021.119725DOI Listing
March 2021

Causal Associations between Serum Urea and Cancer: A Mendelian Randomization Study.

Genes (Basel) 2021 Mar 29;12(4). Epub 2021 Mar 29.

Department of Biochemistry and Cancer Institute of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.

Urea is largely derived from the urea cycle reactions through hepatic detoxification of free ammonia and cleared by urination, and the serum urea level is a crucial medical indicator for measuring the kidney function in patients with nephropathy; however, investigative revelations pointing to the serum urea level as a risk factor for cancer are very scarce, and relevant studies are restricted by potential biases. We aimed to explore the causal relationships of the serum urea level with cancer development by focusing on renal cell carcinoma (RCC) using the Mendelian randomization (MR) analyses. Summary estimates were collected from the inverse-variance weighted (IVW) method based on six single nucleotide polymorphisms (SNPs). The selected SNPs related to the serum urea were obtained from a large genome-wide association study (GWAS) of 13,312 European participants. The summary statistics of RCC were also available from public databases (IARC, = 5219 cases, = 8011 controls). Sensitivity analyses included the weighted median and MR-Egger methods. Serum urea was inversely associated with RCC in females (effect = 1.93; 95% CI: 1.24 to 3.01; = 0.004) but exhibited null association with RCC in males, breast cancer (BRCA) in both genders and prostate cancer (PCa) in males. Similar conclusions were also drawn from the weighted median and MR-Egger. These findings reveal an intriguing link between serum urea and cancer risks for the very first time. Without ambiguity, the serum urea is causatively related to RCC specifically in females, although the mechanism(s) by which urea is involved in RCC development remains to be experimentally/clinically investigated. Our studies may well provide novel insights for RCC diagnosis, intervention and/or therapy.
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http://dx.doi.org/10.3390/genes12040498DOI Listing
March 2021

Effects of Cognitive Behavioral Therapy for Depression and Anxiety, Response Rates and Adverse Events in Patients with Locoregional Advanced Nasopharyngeal Carcinoma.

Integr Cancer Ther 2021 Jan-Dec;20:15347354211006179

Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.

Purpose: This retrospective study investigated the effects of cognitive behavioral therapy (CBT) on depression, anxiety, response rates, and adverse events in patients with locoregional advanced nasopharyngeal carcinoma (NPC).

Methods: A total of 269 patients with diagnosis of stage III-IVA NPC received either CBT plus chemoradiotherapy (CBT group, n = 136) or treatment as usual (TAU) plus chemoradiotherapy (TAU group, n = 133). Patients in the CBT group received a series of 6 CBT sessions for 6 weeks during concurrent chemoradiotherapy. Depression and anxiety were assessed using the Hospital Anxiety and Depression Scale (HADS) score at baseline, the completion of radiotherapy, and 6, 12, and 24 months after radiotherapy. Response rates and adverse events were also evaluated.

Results: Patients in the CBT group showed significantly less depression and anxiety than patients in the TAU group after the completion of radiotherapy ( < .05). Complete response rates were 99.3% (135/136) and 92.5% (123/133) in the CBT group and TAU group with a small effect size (Phi coefficient = .171), respectively ( = .005). Compared with the TAU group, the CBT group showed a significantly lower incidence of acute adverse events and late toxic effects.

Conclusions: The addition of CBT to chemoradiotherapy significantly reduced depressive and anxiety symptoms. CBT combined with chemoradiotherapy is associated with improved response rates, with reduced incidence of toxic effects in patients with locoregional advanced NPC. Based on this study, we registered a randomized controlled clinical trials to better define the role of CBT in patients with locoregional advanced NPC (Registration number: ChiCTR2000034701).
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http://dx.doi.org/10.1177/15347354211006179DOI Listing
March 2021

Visible-to-UVC driven upconversion photocatalyst sterilization efficiency and mechanisms of β-NaYF: Pr, Li@BiOCl with a core-shell structure.

J Environ Manage 2021 Mar 26;288:112394. Epub 2021 Mar 26.

Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China. Electronic address:

UVC (wavelength < 280 nm) has a wide range of applications in the field of water disinfection due to its excellent bactericidal performance. In this work, we synthesized an upconversion luminescent material, β-NaYF: Pr, Li (NYF), which can generate UVC, and compounded it with a BiOCl photocatalyst to synthesize β-NaYF: Pr, Li@BiOCl (NYF-Bi) with a core-shell structure. The NYF-Bi composite material can be driven under visible light and has high photocatalytic activity. The bactericidal performance of NYF and NYF-Bi were evaluated using Escherichia coli, Staphylococcus aureus, Shigella and Salmonella. The NYF-Bi composite material killed 99.99% of E. coli under visible light (λ ≥ 420 nm) within 180 min and maintained high germicidal efficacy after 4 cycles. Finally, we deduced the sterilization mechanism of the NYF-Bi composite material through carrier dynamics studies and catching agent experiments. The death of bacteria was mainly caused by UVC light and active species, including h, OH, and O- 2. This research provides a new material for water disinfection.
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http://dx.doi.org/10.1016/j.jenvman.2021.112394DOI Listing
March 2021

Extraction, characterization of aloe polysaccharides and the in-depth analysis of its prebiotic effects on mice gut microbiota.

Carbohydr Polym 2021 Jun 2;261:117874. Epub 2021 Mar 2.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, and International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu Province, 214122, China. Electronic address:

Despite the well-known health benefits of aloe polysaccharide (APs), little is known about how APs modulate the gut microbiota and the relationship between microbiota and SCFAs. Here, APs was extracted by ultrasound extraction. FT-IR and glycosidic linkage type analysis showed that a major part of APs consisted of → 4)-β-Manp-(1 → residues with acetyl groups. APs supplementation to mice prominently boosted SCFAs-producing Bacteroides and Parabacteria in the feces. On the other hand, it decreased the abundance of Firmicutes and Clostridium. A positive correlation between microbiota and SCFAs was revealed, with Parabacteria and Clostridium being the key microbiota to significantly promote SCFAs. APs promoted the fructose and mannose metabolism by upregulating the gene expression of 17 enzymes, containing fructose-bisphosphate aldolase [EC:4.1.2.13]. Our findings highlight SCFAs-producing Parabacteria were primary degrader of APs, and APs may have prebiotic effects on gut microbiota.
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http://dx.doi.org/10.1016/j.carbpol.2021.117874DOI Listing
June 2021

The present situation of pesticide residues in China and their removal and transformation during food processing.

Food Chem 2021 Mar 12;354:129552. Epub 2021 Mar 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China. Electronic address:

Pesticide residues are one of the most important issues affecting food safety. In this review, the general situation of pesticide residues in fruits and vegetables based on the background of the Chinese fruit and vegetable industry is first described. On the basis of primary processing of agricultural products, the effects of processing methods on the removal and metabolism of pesticide residues are reviewed in this paper. In addition, the transformation mechanism of pesticides in crops and in the environment is discussed. Finally, this study summarizes the development trend of pesticide-residue monitoring methods. With the prohibition of a large number of pesticides in China, the risk of pesticide residues is gradually reduced. However, some highly toxic pesticides can still be detected. Furthermore, the development of high-resolution mass spectrometry screening methods and rapid and intelligent detection instruments is the development trend for pesticide monitoring in the future.
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http://dx.doi.org/10.1016/j.foodchem.2021.129552DOI Listing
March 2021

The Electrophilicity of Surface Carbon Species in the Redox Reactions of CuO-CeO Catalysts.

Angew Chem Int Ed Engl 2021 Mar 17. Epub 2021 Mar 17.

Department of Chemical Engineering, University College London, London, WC1E 7JE, UK.

Electronic metal-support interactions (EMSI) describe the electron flow between metal sites and a metal oxide support. It is generally used to follow the mechanism of redox reactions. In this study of CuO-CeO redox, an additional flow of electrons from metallic Cu to surface carbon species is observed via a combination of operando X-ray absorption spectroscopy, synchrotron X-ray powder diffraction, near ambient pressure near edge X-ray absorption fine structure spectroscopy, and diffuse reflectance infrared Fourier transform spectroscopy. An electronic metal-support-carbon interaction (EMSCI) is proposed to explain the reaction pathway of CO oxidation. The EMSCI provides a complete picture of the mass and electron flow, which will help predict and improve the catalytic performance in the selective activation of CO , carbonate, or carbonyl species in C1 chemistry.
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http://dx.doi.org/10.1002/anie.202102570DOI Listing
March 2021

Anti-fatigue effect of Lepidium meyenii Walp. (Maca) on preventing mitochondria-mediated muscle damage and oxidative stress in vivo and vitro.

Food Funct 2021 Apr 17;12(7):3132-3141. Epub 2021 Mar 17.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Maca (Lepidium meyenii Walp.) has emerged as a popular functional plant food due to its various pharmacological properties, including anti-oxidation, anti-inflammation and anti-fatigue activity. In this study, we investigated the role of Maca aqueous extract (ME) on muscle during exercise-induced fatigue both in vivo and in vitro. As a result, ME significantly enhanced mouse leg grip-strength and increased exercise endurance in the rota-rod test. ME could clear the accumulation of metabolites - blood lactic acid (BLA), blood urea nitrogen (BUN) and reactive oxygen species (ROS) levels after weight-loaded forced swimming. Focusing on muscle, we found that the administration of ME strengthened mouse muscle structures so that exercise-induced metabolic stress was alleviated by upregulating NAD/NADH. Furthermore, ME inhibited the reduction of the viability and accumulation of ROS by treatment with HO in C2C12 skeletal muscle cells. ME-induced activation of energy metabolism in skeletal muscle might up-regulate mitochondrial biogenesis and function, thereby protecting against oxidative stress-induced damage. We concluded that the effects of Maca played a crucial role in the regulation of exercise-induced fatigue in mouse muscle, which could be expected to serve as a functional food supplement for improving exercise performance and alleviating physical fatigue.
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http://dx.doi.org/10.1039/d1fo00383fDOI Listing
April 2021

Preparation and Properties of a Composite Phase Change Energy Storage Gypsum Board Based on Capric Acid-Paraffin/Expanded Graphite.

ACS Omega 2021 Mar 25;6(9):6144-6152. Epub 2021 Feb 25.

The Laboratory of Architectural Environment and Energy Application Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi Province, China.

Energy has become the key material basis of social development. In this work, liquid capric acid-paraffin was evenly adsorbed in the pore structure of expanded graphite (EG) by a physical adsorption method, and the new composite phase change material of capric acid-paraffin/expanded graphite (CA-P/EG) was prepared. The Fourier transform infrared (FT-IR) curves of CA-P/EG composites did not change after 1000 cycles, and there was no new characteristic absorption peak, indicating that CA-P/EG composites have good chemical stability. The results showed that the optimum content of CA-P/EG in a phase change energy storage gypsum board was 20%, and the wet bending strength and compressive strength were 2.42 and 6.45 MPa, respectively. The water absorption was 16.37%, and the apparent density was 1.410 g/cm. In addition, the melting and freezing temperatures were 26.40 and 23.10 °C, and the latent heats of melting and freezing were 27.20 and 25.69 J/g, respectively. It was found that the gypsum board has excellent thermal stability after 400 times of melting-freezing cycling and that the heat storage capacity increases with the increase of the CA-P/EG content and the thickness of the gypsum board.
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http://dx.doi.org/10.1021/acsomega.0c05058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948235PMC
March 2021

Carotenoids in ameliorate diabetic nephropathy in mice through attenuating oxidative stress.

Biol Chem 2021 Mar 15. Epub 2021 Mar 15.

School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.

Diabetic nephropathy (DN) is the major life-threatening complication of diabetes, and oxidative stress takes part in its initiation and development. This study was performed to evaluate the effects of carotenoids from (CSP) on the renal function and oxidative stress status of mice with streptozotocin (STZ)-induced DN. The results indicated that CSP significantly attenuated symptoms of STZ-induced DN shown by decreased fasting blood glucose, reduced urine volume, urine albumin, serum creatinine and serum urea nitrogen, and improved kidney histological morphology. Furthermore, biochemical analysis of serum and kidney revealed a marked increase in oxidative stress of DN mice as evidenced by reduced total antioxidant capacity (T-AOC), decreased activity of antioxidant enzyme -superoxide dismutase (SOD) and increased level of malondialdehyde (MDA). However, treatment with CSP improved oxidative stress status in DN mice as compared with the mice in model group. Exploration of the potential mechanism validated that CSP ameliorated the oxidative stress status in DN mice by activating the expressions of Nrf2, NQO-1, HO-1, GST and CAT in kidney. These data revealed that CSP may retard the progression of DN by ameliorating renal function, improving the oxidative stress status and activating the Nrf2/ARE pathway.
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http://dx.doi.org/10.1515/hsz-2021-0127DOI Listing
March 2021

Bioactive compound from the Tibetan turnip (Brassica rapa L.) elicited anti-hypoxia effects in OGD/R-injured HT22 cells by activating the PI3K/AKT pathway.

Food Funct 2021 Apr 12;12(7):2901-2913. Epub 2021 Mar 12.

Department of School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Cerebral stroke, a common clinical problem, is the predominant cause of disability and death worldwide. Its prevalence increases and infarctions exacerbate with age. A Tibetan plant, Brassica rapa L., possesses multiple medicinal effects, such as anti-altitude sickness, anti-hyperlipidemia and anti-fatigue, as mentioned in the noted ancient Tibet pharmacopeia "The Four Medical Tantras". Our preliminary studies also showed the anti-hypoxia protection mechanism of B. rapa L., implying its possible relationship with anti-ischemic neuroprotection. However, the potential molecular mechanism of the active constituent of turnip against cerebral ischemia/reperfusion remains unclear. In our study, oxidative stress markers, including LDH, ROS, SOD, GPx and CAT were assayed. In controlled in vitro assays, we found that the turnip's active constituent had remarkable anti-hypoxia capability. We further showed the profound effects of the active constituent of turnip on the levels of apoptosis-related proteins, including Bax, Bcl-2 and caspase-3, which contributed to its anti-inflammatory activity. Western blot analysis results also implied that active-constituent pretreatment reversed the diminished expression of the PI3K/Akt/mTOR pathway mediated by oxygen glucose deprivation/reperfusion (OGD/R); further experimental evidence showed that the protective role was limited in the PI3K inhibitor (LY294002) treatment group. Our results demonstrated that the functional monomer of B. rapa L. exerted a neuroprotective effect against OGD/R-induced HT22 cell injury, and its potential mechanism provides a scientific basis for future clinical applications and its use as a functional food.
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http://dx.doi.org/10.1039/d0fo03190aDOI Listing
April 2021

Heterologous prime-boost: breaking the protective immune response bottleneck of COVID-19 vaccine candidates.

Emerg Microbes Infect 2021 Dec;10(1):629-637

National Institutes for Food and Drug Control, Beijing, People's Republic of China.

COVID-19 vaccines emerging from different platforms differ in efficacy, duration of protection, and side effects. To maximize the benefits of vaccination, we explored the utility of employing a heterologous prime-boost strategy in which different combinations of the four types of leading COVID-19 vaccine candidates that are undergoing clinical trials in China were tested in a mouse model. Our results showed that sequential immunization with adenovirus vectored vaccine followed by inactivated/recombinant subunit/mRNA vaccine administration specifically increased levels of neutralizing antibodies and promoted the modulation of antibody responses to predominantly neutralizing antibodies. Moreover, a heterologous prime-boost regimen with an adenovirus vector vaccine also improved Th1-biased T cell responses. Our results provide new ideas for the development and application of COVID-19 vaccines to control the SARS-CoV-2 pandemic.
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http://dx.doi.org/10.1080/22221751.2021.1902245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009122PMC
December 2021

A Survey of Survival Outcomes for Targeted Cancer Drugs Approved by the US Food and Drug Administration.

Ther Innov Regul Sci 2021 Mar 8. Epub 2021 Mar 8.

School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.

Background: The last two decades have witnessed the vigorous development of targeted cancer drugs and the potent therapeutic effects of these drugs have been validated by various true and surrogate end points. Overall survival (OS) and progression-free survival (PFS) outcomes were two important end points used in targeted cancer drugs clinical trials but investigation on which was rare as a consequence of inherent heterogeneity and complexity. Here, we present the review and analysis of OS and PFS outcomes of all targeted cancer drugs approved by the U.S. Food and Drug Administration (FDA) through December 31, 2019, in the setting of clinical studies.

Methods: The targeted cancer drug directory was accessed via NCI website. The survival outcomes of those drugs in the setting of clinical trials were collected from publicly available Package Inserts and ClinicalTrials.gov. Median overall (OS) and progression-free survival (PFS) outcomes were summarized for targeted cancer drugs that were evaluated as monotherapies in clinical trials, contributions of targeted drugs to combination therapies in terms of survival benefit were analyzed, survival outcomes of FDA-approved first-line targeted therapies in different cancers were investigated, and the correlation between the absolute OS gain (ΔOS) and PFS gain (ΔPFS) as well as the hazard ratios for PFS (HR) and OS (HR) between comparative arms of available randomized clinical trials was evaluated.

Results: A total of 223 indications for 126 targeted cancer drugs have been approved by the FDA through December 31, 2019, among which 28 indications of 23 drugs have been approved for first-line therapies. Eighty indications for leukemia, lymphoma, and rare cancer types without survival data were excluded in the investigation of survival outcomes. For the remaining 143 indications, 99 were approved as monotherapies and 72 were approved as combination therapies. Among monotherapy subset, 18 of 72 (25%) indications with available OS outcomes have maximum median OS less than 12 months, 55 of 72 (76%) drug indications have maximum median OS less than 24 months, and 67 of 72 (93%) have maximum median OS less than 36 months. Regarding PFS, 38 of 88 (43%) drug indications have maximum median PFS less than 6 months, 69 of 88 (78%) drug indications have maximum median PFS less than 12 months, and 84 of 88 (95%) drug indications have maximum median PFS less than 24 months. The addition of targeted drugs to combination regimens under FDA's approval provided notable survival benefit, but the median value of OS gain rate of the available combination regimens was lower than that of PFS. The univariate Spearman Rank correlation coefficient suggested a significant positive correlation between ΔOS and ΔPFS (R = 0.62) but a weak correlation between HR and HR (R = 0.11) in 46 randomized clinical trials that investigated contributions of targeted cancer drugs to combination therapies with available data. A moderate correlation between ΔOS and ΔPFS (R = 0.43) and a rather weak correlation between HR and HR (R = 0.07) were also found in other randomized clinical trials that included in our study with available data.

Conclusions: The survival benefit provided by targeted cancer drugs varied a lot among cancer types. Though targeted cancer drugs showed improvements in both OS and PFS in approved combination regimens, the median value of OS gain rate was lower than that of PFS. As only weak correlation was found between HR and HR, the evidence supporting the use of PFS as a surrogate to OS in the setting of targeted cancer drugs might also be limited.
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http://dx.doi.org/10.1007/s43441-021-00264-1DOI Listing
March 2021

Over 200 million students being taught online in China during COVID-19: Will online teaching become the routine model in medical education?

Asian J Surg 2021 04 22;44(4):672. Epub 2021 Feb 22.

Department of Surgery, West China Hospital, Sichuan University, Chengdu, 610041, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.01.034DOI Listing
April 2021

Facilitating the Deprotonation of OH to O through Fe -Induced States in Perovskite LaNiO Enables a Fast Oxygen Evolution Reaction.

Small 2021 Mar 3;17(12):e2006930. Epub 2021 Mar 3.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P.R. China.

Aliovalent doping is widely adopted to tune the electronic structure of transition-metal oxides for design of low-cost, active electrocatalysts. Here, using single-crystalline thin films as model electrocatalysts, the structure-activity relationship of Fe states doping in perovskite LaNiO for oxygen evolution reaction (OER) is studied. Fe state is found to be crucial for enhancing the OER activity of LaNiO , dramatically increasing the activity by six times, while Fe has negligible effect. Spectroscopic studies and DFT calculations indicate Fe states enhance the degree of Ni/Fe 3d and O 2p hybridization, and meanwhile produce down-shift of the unoccupied density of states towards lower energies. Such electronic features reduce the energy barrier for interfacial electron transfer for water oxidization by 0.2 eV. Further theoretical calculations and H/D isotope experiments reveal the electronic states associated with Fe -O -Ni configuration accelerate the deprotonation of *OH to *O (rate-determining step), and thus facilitate fast OER kinetics.
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http://dx.doi.org/10.1002/smll.202006930DOI Listing
March 2021

Exploration of the SAR Connection between Morphinan- and Arylacetamide-Based κ Opioid Receptor (κOR) Agonists Using the Strategy of Bridging.

ACS Chem Neurosci 2021 Mar 2;12(6):1018-1030. Epub 2021 Mar 2.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.

κ opioid receptor (κOR) is a subtype of opioid receptors, and there are two major κOR agonists currently available, morphinans and arylacetamides, which are structurally distinct from each other. Numerous efforts had been made to correlate these series of compounds in order to establish a consensus binding pattern for κOR agonists. Unfortunately, no morphinan-based agent with an arylacetamidyl substituent has been identified as a κOR agonist with a pharmacological profile similar to arylacetamides. Since the recently described morphinan-based compound SLL-039 was identified as a selective and potent κOR agonist that contains a unique benzamidyl substituent in structure similar to arylacetamides, numerous arylacetamidyl substituents were introduced to this scaffold to examine whether the structure-activity relationships (SARs) of arylacetamides in conferring κOR agonistic activities could be reproduced by these analogues. Thus, a series of -cyclopropylmethyl-7α-arylacetamidylphenyl-6,14-endoethanotetrahydronorthebaine analogues were designed, synthesized, and assayed for biological activities. Among these compounds, compound with a 3',4'-dimethylphenylacetamidyl substituent showed a single digit low nanomolar affinity to the κOR and relatively high subtype selectivity in binding assays, but this profile was not reproduced in functional assays. In contrast, compound displayed moderately selective κOR agonistic activities in functional assays, which was inconsistent with its nonselective nature in binding assays. Overall, introduction of an arylacetamidyl substituent to the morphinan-based scaffold was associated with pharmacological diversity in both binding and functional activities on opioid receptors . The resultant SARs were inconsistent with that of classical arylacetamides as κOR agonists, despite bearing a similar arylacetamidyl substituent in the structure. Therefore, the arylacetamidyl substituent of the morphinan-based scaffold was found to be disconnected from that of arylacetamides in conferring κOR activities.
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http://dx.doi.org/10.1021/acschemneuro.1c00034DOI Listing
March 2021

Establishing a rare diseases center: Experiences from Western China.

Authors:
Li Gong Qian He

Intractable Rare Dis Res 2021 Feb;10(1):60-61

Department of Outpatient, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Rare diseases pose unique challenges to health care delivery. In August 2016, the West China Hospital of Sichuan University (WCHSU) established a rare diseases center. This center has created a multidisciplinary team of rare disease experts. The center provides expedited pathways online and offline for patients with rare diseases to save them time and money, to improve their experience, and to increase the hospital's efficiency. At the same time, the center regularly organizes public education campaigns and it offers free consultations to enhance awareness of rare diseases. Establishment of the rare disease alliance and facilitation of 5G-based remote multi-disciplinary consultations will help to improve the level of diagnosis and treatment and to solve problems with diagnosis and treatment encountered by local patients with rare diseases. WCHSU's rare diseases center has been feasible, acceptable, and effective in Western China and it should benefit patients, doctors, and hospitals. The center should lead to significant improvements in treatment for patients with rare diseases. The successful establishment of a rare diseases center here may be a useful reference for other parts of the world.
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http://dx.doi.org/10.5582/irdr.2020.03091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882083PMC
February 2021

Practice in Information Technology Support for Fangcang Shelter Hospital during COVID-19 Epidemic in Wuhan, China.

J Med Syst 2021 Feb 19;45(4):42. Epub 2021 Feb 19.

Information Center, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.

In confronting the sudden epidemic of COVID-19, China and other countries have been under great deal of pressure to block virus transmission and reduce death cases. Fangcang shelter hospital, which is converted from large-scale public venue, is proposed and proven to be an effective way for administering medical care and social isolation. This paper presents the practice in information technology support for a Fangcang shelter hospital in Wuhan, China. The experiences include the deployment strategy of IT infrastructure, the redesign of function modules in the hospital information system (HIS), equipment maintenance and medical staff training. The deployment strategy and HIS modules have ensured smoothness and efficiency of clinical work. The team established a quick response mechanism and adhered to the principle of nosocomial infection control. Deployment of network and modification of HIS was finished in the 48 hours before patient admittance. A repair hotline and remote support for equipment and software were available whenever medical workers met with any questions. No engineer ever entered the contaminated areas and no one was infected by the coronavirus during the hospital operation. Up to now, Fangcang shelter hospital is adopted by many regions around the world facing the collapse of their medical systems. This valuable experience in informatization construction and service in Wuhan may help participators involving in Fangcang shelter hospital get better information technology support, and find more practical interventions to fight the epidemic.
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http://dx.doi.org/10.1007/s10916-021-01721-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894969PMC
February 2021

NiCo-Layered Double Hydroxide-Derived B-Doped CoP/NiP Hollow Nanoprisms as High-Efficiency Electrocatalysts for Hydrogen Evolution Reaction.

ACS Appl Mater Interfaces 2021 Mar 17;13(8):9932-9941. Epub 2021 Feb 17.

College of Chemical Engineering, Fuzhou University, Xueyuan Road No. 2, Fuzhou 350116, China.

Rational design and controllable synthesis of multiple metal components according to chemical composition and morphology are essential for obtaining desirable electrochemical performance for efficient hydrogen production because of the morphology and synergistic effects of different components. Herein, we report an approach to facilely fabricate bimetal compounds with a well-defined hollow nanoprism structure using a self-templated strategy to synthesize novel hierarchical NiCo-layered double hydroxide (NiCo-LDH) nanosheets as precursors followed by in situ phosphorization. Among the as-synthesized products of different mole ratios of Ni/Co, the NiCo-B-P nanoprisms that integrate the advantages of a hollow structure, an optimal Ni-Co synergistic effect, and a unique B-doped CoP/NiP bimetallic phosphide derived from NiCo-LDH nanosheets exhibit excellent hydrogen evolution reaction (HER) activity in an alkaline solution at 10 mA cm with the lowest overpotential of 78 mV and long-term stability. This study may offer an appropriate structure and compositional design of bimetallic alkaline HER catalysts.
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http://dx.doi.org/10.1021/acsami.0c20294DOI Listing
March 2021

Difference in the binding mechanism of distinct antimony forms in bovine serum albumin.

Biometals 2021 Feb 15. Epub 2021 Feb 15.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, People's Republic of China.

The toxicity of antimony (Sb) is closely related to its chemical forms. To further realize the toxicity risk of different forms of Sb, the separate and simultaneous binding mechanisms of antimony potassium tartrate/potassium pyroantimonate with bovine serum albumin (BSA) were investigated with muti-spectroscopic methods. Fluorescence quenching result and UV-vis absorption spectra showed that a 1:1 complex was formed between antimony potassium tartrate/potassium pyroantimonate and BSA through a modest binding force. The results revealed that the binding of antimony potassium tartrate/potassium pyroantimonate to BSA caused changes in the secondary structure of BSA. Both Sb forms (antimony potassium tartrate and potassium pyroantimonate) were able to interact with BSA when coexisting but there was a binding influence on their interacting with the BSA. Both Sb forms interfere with the binding of the other to protein.
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http://dx.doi.org/10.1007/s10534-021-00291-3DOI Listing
February 2021

OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in glioma.

J Mol Cell Biol 2021 Jan 28. Epub 2021 Jan 28.

The Brain Science Center, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.

Transcription factors (TFs) control an array of expressed genes. However, the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown. Here, in TRPC6-regulated proline oxidase 1 (POX) transcription in human glioma, we report that OIP5-AS1, a noncoding RNA, determines the specificity of p53-driven POX expression. The OIP5-AS1/p53 complex via its 24 nucleotides binds to the POX promoter and is necessary for POX expression but not for p21 transcription. An O-site in the POX promoter to which OIP5-AS1 binds was identified that is required for OIP5-AS1/p53 binding and POX transcription. Blocking OIP5-AS1 binding to the O-site inhibits POX transcription and promotes glioma development. Thus, the OIP5-AS1/O-site module decides p53-controlled POX expression as regulated by TRPC6 and affects glioma development.
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http://dx.doi.org/10.1093/jmcb/mjab001DOI Listing
January 2021

Study on fecal fermentation characteristics of aloe polysaccharides in vitro and their predictive modeling.

Carbohydr Polym 2021 Mar 5;256:117571. Epub 2021 Jan 5.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, and International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China. Electronic address:

Aloe polysaccharides (APs) are well-known plant polysaccharides, but little is known about their digestion and fermentation characteristics in vitro. In this study, the molecular weight of APs had no significant changes after gastric and intestinal digestion. During the fecal fermentation, the content of volatiles and pH value decreased continuously, while the short-chain fatty acids (SCFAs) concentration increased significantly. Additionally, the abundance of the microbiota associated with the metabolism of SCFAs was increased, including Prevotella, Catenibacterium, Lachnospiraceae, and Coprococcus, while the harmful microbiota was decreased, like Escherichia-Shigella, and Veillonella. Moreover, bioinformatics analysis indicated that APs boosted fructose and mannose metabolism, and the gene expressions of enzymes, containing mannose-6-phosphate isomerase [EC:5.3.1.8]. Structural equation modeling also highlighted that SCFAs-producing microbiota were primary degraders of APs, suggesting APs may facilitate the manufacture of functional foods with the purpose of maintaining intestinal health.
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http://dx.doi.org/10.1016/j.carbpol.2020.117571DOI Listing
March 2021

Dynamic memristor-based reservoir computing for high-efficiency temporal signal processing.

Nat Commun 2021 01 18;12(1):408. Epub 2021 Jan 18.

Institute of Microelectronics, Beijing Innovation Center for Future Chips (ICFC), Tsinghua University, 100084, Beijing, China.

Reservoir computing is a highly efficient network for processing temporal signals due to its low training cost compared to standard recurrent neural networks, and generating rich reservoir states is critical in the hardware implementation. In this work, we report a parallel dynamic memristor-based reservoir computing system by applying a controllable mask process, in which the critical parameters, including state richness, feedback strength and input scaling, can be tuned by changing the mask length and the range of input signal. Our system achieves a low word error rate of 0.4% in the spoken-digit recognition and low normalized root mean square error of 0.046 in the time-series prediction of the Hénon map, which outperforms most existing hardware-based reservoir computing systems and also software-based one in the Hénon map prediction task. Our work could pave the road towards high-efficiency memristor-based reservoir computing systems to handle more complex temporal tasks in the future.
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http://dx.doi.org/10.1038/s41467-020-20692-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814066PMC
January 2021

A Patient Journey Map to Improve the Home Isolation Experience of Persons With Mild COVID-19: Design Research for Service Touchpoints of Artificial Intelligence in eHealth.

JMIR Med Inform 2021 Apr 12;9(4):e23238. Epub 2021 Apr 12.

Department of Design Organisation & Strategy, Faculty of Industrial Design Engineering, Delft University of Technology, Delft, Netherlands.

Background: In the context of the COVID-19 outbreak, 80% of the persons who are infected have mild symptoms and are required to self-recover at home. They have a strong demand for remote health care that, despite the great potential of artificial intelligence (AI), is not met by the current services of eHealth. Understanding the real needs of these persons is lacking.

Objective: The aim of this paper is to contribute a fine-grained understanding of the home isolation experience of persons with mild COVID-19 symptoms to enhance AI in eHealth services.

Methods: A design research method with a qualitative approach was used to map the patient journey. Data on the home isolation experiences of persons with mild COVID-19 symptoms was collected from the top-viewed personal video stories on YouTube and their comment threads. For the analysis, this data was transcribed, coded, and mapped into the patient journey map.

Results: The key findings on the home isolation experience of persons with mild COVID-19 symptoms concerned (1) an awareness period before testing positive, (2) less typical and more personal symptoms, (3) a negative mood experience curve, (5) inadequate home health care service support for patients, and (6) benefits and drawbacks of social media support.

Conclusions: The design of the patient journey map and underlying insights on the home isolation experience of persons with mild COVID-19 symptoms serves health and information technology professionals in more effectively applying AI technology into eHealth services, for which three main service concepts are proposed: (1) trustworthy public health information to relieve stress, (2) personal COVID-19 health monitoring, and (3) community support.
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http://dx.doi.org/10.2196/23238DOI Listing
April 2021

Multiwavelet scale multidimensional recurrence quantification analysis.

Chaos 2020 Dec;30(12):123109

School of Science, Beijing Information Science and Technology University, Beijing 100192, People's Republic of China.

The multiwavelet scale multidimensional recurrence quantification analysis (MWMRQA) method is proposed in this paper, which is a combination of multidimensional recurrence quantification analysis and wavelet packet decomposition. It allows us to quantify the recurrence properties of a single multidimensional time series under different wavelet scales. We apply the MWMRQA method to the Lorenz system and the Chinese stock market, respectively, and show the feasibility of this method as well as the dynamic variation of the Lorenz system and the Chinese stock market under different wavelet scales. This provides another perspective for other disciplines that need to study the recurrence properties of different scales in the future.
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http://dx.doi.org/10.1063/5.0025882DOI Listing
December 2020

Mental health conditions among the general population, healthcare workers and quarantined population during the coronavirus disease 2019 (COVID-19) pandemic.

Psychol Health Med 2020 Dec 30:1-13. Epub 2020 Dec 30.

Psychiatry and Pharmacology, University of Toronto , Toronto, Canada.

This study sought to assess the differences in mental health conditions among the general population, quarantined population and healthcare workers during the COVID-19 outbreak in China. An online rapid assessment captured depressive and anxiety symptoms, and sleep quality data. A total of 2689 participants (n=374 general population, n=403 healthcare workers, n=1912 quarantined population) were included in the final statistical analysis. The proportion of individuals with mild and/or serious depression and anxiety were higher in the general population when compared to the quarantined population and healthcare workers (58.6% vs. 25.1% 48.6%, <0.001; 41.2% 18.5%  35.7%, <0.001). The prevalence of sleep disturbance was higher among healthcare workers than the general population and quarantined population (29.8%  24.1%  22.7%, =0.013). Logistic regression analysis showed that, perceived effect on daily life was associated with depression, anxiety and sleep disturbance in the general population, quarantined population and the healthcare workers. The general population had a greater risk of developing psychological problems. The healthcare workers suffered the poorest sleep quality. Future research must further explorethe targeted measures for the general population and healthcare workers while combating COVID-19.
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http://dx.doi.org/10.1080/13548506.2020.1867320DOI Listing
December 2020

Spread of tet(X5) and tet(X6) genes in multidrug-resistant Acinetobacter baumannii strains of animal origin.

Vet Microbiol 2021 Feb 13;253:108954. Epub 2020 Dec 13.

National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. Electronic address:

The recent emergence of plasmid-mediated tigecycline resistance gene tet(X) has challenged the clinical effectiveness of tigecycline as a last-resort treatment option. During 2017-2018, 336 fecal samples from sick ducks, pigs, chickens and geese in Guangdong, China, were screened for tet(X)-positive Acinetobacter baumannii strains. Their activities on tetracyclines were determined by microbiological degradation and mass spectrometry, followed by susceptibility testing, sequence typing, gene transfer, molecular location and genomic DNA sequencing analyses. A total of 10 tet(X)-positive A. baumannii strains were isolated from ducks and chickens, including eight plasmid-borne tet(X5)-positive and two chromosomal tet(X6)-positive isolates. All of them exhibited good degradation activities on tetracyclines by hydroxylation at C11a and were multidrug-resistant to tigecycline, tetracycline, florfenicol, ciprofloxacin and trimethoprim/sulfamethoxazole. Genetically, they belonged to two sequence types (ST355, n = 8; ST1980, n = 2) that were consistent with their pulsotypes, revealing a clonal spread of ST355 A. baumannii. An ISCR2- or IS26-mediated tet(X) transposition structure, homologous to those of clinical A. baumannii strains, was also identified and ISCR2 could transfer tet(X5) into the recipient Acinetobacter baylyi ADP1 at a frequency of (1.8 ± 0.3)×10. Therefore, more efforts are needed to evaluate the clinical impact of these tigecycline resistance genes.
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http://dx.doi.org/10.1016/j.vetmic.2020.108954DOI Listing
February 2021

Ca/calmodulin-dependent protein kinase IV attenuates inflammation and mitochondrial dysfunction under insulin resistance in C2C12 cells.

Authors:
Jiali Liu Qian He

Arch Physiol Biochem 2020 Dec 28:1-10. Epub 2020 Dec 28.

Department of Clinical Laboratory, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, Shaanxi, China.

CaMKIV has been reported involved in the improvement of whole-body insulin sensitivity and mitochondrial biogenesis of skeletal muscle. Here, we first investigate the effects of CaMKIV on glucose metabolism, cell viability, inflammatory function, and mitochondrial function in palmitate-induced C2C12 cells of insulin resistance. Then we explored the potential mechanism of these effects. Differentiated C2C12 cells were treated with or without 100 ng/ml of CaMKIV under palmitate-induced insulin resistance. The results suggest palmitate induced insulin sensitivity, reduced glucose uptake, decreased cell viability, increased inflammatory factors, and caused mitochondrial dysfunction in C2C12 cells. Of note, CaMKIV reversed palmitate-induced insulin resistance, increased the reduction of glucose uptake, inhibited inflammatory response, and mitochondrial dysfunction, despite of no change in cells viabilities. However, these beneficial effects of CaMKIV were blocked by the downregulation of CREB1. Taken together, our data demonstrated CaMKIV prevents palmitate-induced insulin resistance, inflammatory response, and mitochondrial dysfunction through phosphorylated CREB1 in differentiated C2C12 cells.
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http://dx.doi.org/10.1080/13813455.2020.1861028DOI Listing
December 2020