Publications by authors named "Qian Han"

232 Publications

Correlation between bone volume fraction in posterior implant area and initial implant stability.

Oral Surg Oral Med Oral Pathol Oral Radiol 2021 Aug 18. Epub 2021 Aug 18.

Resident Doctor, First Affiliated Hospital of the University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Objective: Bone volume fraction (BV/TV) was used as a quantitative indicator of bone mineral density (BMD) in the posterior dental implant area, and the relationship between it and the initial stability of the implant was analyzed.

Methods: 53 patients with posterior dental implantation were selected. Remove the bone fragments from the planting area and put them in the formalin solution. Then, the implant was placed after the operator prepared the hole step by step. Peak Insertion torque value (ITV) and Implant stability quotient (ISQ) were recorded. The BV/TV of the bone fragments was measured by Image pro Plus6.0. The correlation between it and ITV and ISQ was analyzed.

Results: BV/TV was correlated with ITV and ISQ in the posterior dental planting area (r=0.862, r=0.832, P < 0.05). The correlation of the parameters in the posterior part of maxilla was higher than that in the mandible. There were significant differences in BV/TV, ITV, and ISQ between the mandible and maxilla, and the implant area of the mandible was higher than that of the maxilla.

Conclusion: BV/TV can provide an objective index for BMD in the implant area, which has an important influence on the evaluation of the initial stability of the implant.
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http://dx.doi.org/10.1016/j.oooo.2021.08.008DOI Listing
August 2021

Ultrasensitive dual-quenching electrochemiluminescence immunosensor for prostate specific antigen detection based on graphitic carbon nitride quantum dots as an emitter.

Mikrochim Acta 2021 Sep 23;188(10):350. Epub 2021 Sep 23.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.

Early monitoring of prostate-specific antigen (PSA) is crucial in diagnosis and proactive treatment of prostate disease. Herein, a dual-quenching ternary ECL immunosensor was designed for PSA detection based on graphitic carbon nitride quantum dots (g-CNQDs, as an emitter), potassium persulfate (KSO, as a coreactant), and silver nanoparticles doped multilayer TiC MXene hybrids ([email protected], as a coreaction accelerator). First, [email protected] was immobilized on the surface of a glassy carbon electrode, then g-CNQDs was further adsorbed on [email protected] to acquire a higher initial ECL signal at a potential window from - 1.3 to 0.0 V (vs. Ag/AgCl). [email protected] not only acted as the coreaction accelerator, but also as a matrix to load enormous g-CNQDs and prostate-specific capture antibody via Ag-N bond. Meanwhile, prostate-specific detection antibody was marked by gold nanoparticles modified manganese dioxide as a dual-quenching probe (Ab2- [email protected]). When [email protected] was introduced into the ternary ECL system via sandwiched immuno-reaction, the high-sensitive detection of PSA was achieved by the dual-quenching effect, caused by the resonant energy transfer from g-CNQDs (energy donor) to [email protected] (energy acceptor). As a result, this ECL immunosensor showed a good dynamic concentration range from 10 fg·mL to 100 ng·mL with a detection limit of 6.9 fg·mL for PSA detection. The dual-quenching ECL strategy presented high stability and good specificity to open up a new pathway for ultrasensitive immunoassay.
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http://dx.doi.org/10.1007/s00604-021-05015-5DOI Listing
September 2021

Novel optoelectronic metal organic framework material perylene tetracarboxylate magnesium: preparation and biosensing.

Nanoscale 2021 Sep 22. Epub 2021 Sep 22.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

The pursuit for improving photoelectrochemical (PEC) performances of organic materials remains an urgent need. Here, we have proposed an envision of the preparation the metal-organic frameworks (MOFs) with arenes to realize high photo-to-current conversion efficiency and excellent PEC performances. Magnesium 3,4,9,10-perylene tetracarboxylic acid metal-organic frameworks (Mg-PTCA MOFs) were synthesized for the first time. The uniformly distributed and regular-shaped Mg-PTCA MOFs showed a much more stable and higher photocurrent than the single PTCA and its derivatives, which confirmed our hypothesis. A regenerated-biosensor was designed for microRNA analysis based on Mg-PTCA MOFs as a novel photoelectric material, target-triggered three-dimensional DNA Scaffold (3D-Sca) as an efficient signal amplifier, and gold nanoclusters (Au NCs) as quencher. The elaborately designed biosensor achieved ultrasensitive detection for miRNA 21 with a dynamic range from 10 aM to 10 pM and a detection limit of 2.8 aM. This biosensor showed good analytical performance in the extracts of different cancer cells, indicating the possibility for early diagnosis, timely staging assessment, and accurate prognostic judgment for diseases. The recommendable performances of Mg-PTCA MOFs highlight the significance of organic MOFs in PEC sensing.
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http://dx.doi.org/10.1039/d1nr03300jDOI Listing
September 2021

The choice of anesthesia for acute abdomen surgery patients and its influence on gastrointestinal function recovery.

Am J Transl Res 2021 15;13(8):9621-9626. Epub 2021 Aug 15.

Department of Interventional Radiology, Cangzhou Central Hospital 16 West Xinhua Road, Yunhe District, Cangzhou, China.

Objective: To study the clinical anesthesia options for patients undergoing acute abdomen surgery and its effect on the recovery of patients' gastrointestinal function.

Methods: 120 patients who underwent abdomen surgery in our hospital from January 2018 to January 2019 were recruited as the research cohort and placed into group A (n=40) or group B (n=80) according to different anesthesia method each underwent. Group A was administered combined spinal-epidural anesthesia, and group B was administered general anesthesia with tracheal intubation. The anesthesia-related time indicators, the postoperative analgesia, the complication rates (CR), the gastric function indicators, and the gastrointestinal function recovery times were compared between the two groups.

Results: Group A's anesthesia-related time indicators were significantly lower than group B's (P<0.001). The visual analogue scale (VAS) postoperative pain score in group A was (2.21±0.41), which was observably lower than the corresponding score in group B (P<0.001). There was no significant difference in the CR between the two groups (7.5% vs 17.5%) (P>0.05). The motilin and ghrelin levels in group A were significantly higher than they were in group B (P<0.05). The gastrointestinal function recovery time in group A was notably less than it was in group B (P<0.05).

Conclusion: Spinal-epidural anesthesia is a preferred technique because of its strengths in gastric function and operation success rates by taking patients' actual situations into consideration.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430078PMC
August 2021

Prevalence of Latent Tuberculosis Infection Among Healthy Young Children and Adolescents and a Two-step Approach for the Diagnosis of Tuberculosis Infection in Chengdu, China.

Pediatr Infect Dis J 2021 Sep 9. Epub 2021 Sep 9.

From the Department of Pediatrics, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education; Chengdu Chenghua District Maternal and Child Health Hospital; Department of Pediatrics, Chengdu Second People's Hospital; and Department of Ultrasound, West China Second University Hospital, Sichuan University, Chengdu, China.

Background: China has a high burden of tuberculosis and latent tuberculosis infection (LTBI). The aim of this study was to estimate the prevalence of LTBI among healthy young children and adolescents and test a 2-step approach to explore the threshold for the diagnosis of tuberculosis infection in Chengdu, China.

Methods: Healthy preschool children and school-going children in Chengdu, Sichuan Province, were screened for LTBI using the tuberculin skin test (TST). Preschool children with TST ≥ 5 mm also underwent interferon-γ release assay (IGRA) to explore the threshold of this 2-step approach.

Results: In total, 5667 healthy young children and adolescents completed TST test between July 2020 and January 2021 and were included in the present analysis. The age of the participants ranged from 2.4 to 18 years (median 7.25 ± 4.514 years), of which 2093 (36.9%) were younger than 5 years. The overall prevalence of LTBI was 6.37% and 6.64% in children younger than 5 years old. Fourteen of the 341 preschool children with TST ≥5 mm were interferon-γ release assay positive, of which 4 showed a TST result of 5-10 mm, and 6 preschool children received preventive treatment for LTBI.

Conclusions: Healthy young children and adolescents should also be considered as important target populations for LTBI screening. TST can be recommended for first-line screening as part of a 2-step approach for LTBI screening using a positive threshold of 5 mm.
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http://dx.doi.org/10.1097/INF.0000000000003338DOI Listing
September 2021

Protein kinase G signaling pathway is involved in sympathetically maintained pain by modulating ATP-sensitive potassium channels.

Reg Anesth Pain Med 2021 Sep 7. Epub 2021 Sep 7.

Department of Radiation Biology, Fourth Military Medical University, Xi'an, China

Background: Sympathetically maintained pain (SMP) involves an increased excitability of dorsal root ganglion (DRG) neurons to sympathetic nerve stimulation and circulating norepinephrine. The current treatment of SMP has limited efficacy, and hence more mechanistic insights into this intractable pain condition are urgently needed.

Methods: A caudal trunk transection (CTT) model of neuropathic pain was established in mice.Immunofluorescence staining, small interfering RNA, pharmacological and electrophysiological studies were conducted to test the hypothesis that norepinephrine increases the excitability of small-diameter DRG neurons from CTT mice through the activation of cyclic guanosine monophosphate-protein kinase G (cGMP-PKG) signaling pathway.

Results: Behavior study showed that CTT mice developed mechanical and heat hypersensitivities, which were attenuated by intraperitoneal injection of guanethidine. CTT mice also showed an abnormal sprouting of tyrosine hydroxylase-positive nerve fibers in DRG, and an increased excitability of small-diameter DRG neurons to norepinephrine, suggesting that CTT is a useful model to study SMP. Importantly, inhibiting cGMP-PKG pathway with small interfering RNA and KT5823 attenuated the increased sympathetic sensitivity in CTT mice. In contrast, cGMP activators (Sp-cGMP, 8-Br-cGMP) further increased sympathetic sensitivity. Furthermore, phosphorylation of ATP-sensitive potassium channel, which is a downstream target of PKG, may contribute to the adrenergic modulation of DRG neuron excitability.

Conclusions: Our findings suggest an important role of cGMP-PKG signaling pathway in the increased excitability of small-diameter DRG neurons to norepinephrine after CTT, which involves an inhibition of the ATP-sensitive potassium currents through PKG-induced phosphorylation. Accordingly, drugs targeting this pathway may help to treat SMP.
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http://dx.doi.org/10.1136/rapm-2021-102539DOI Listing
September 2021

Pregabalin and Gabapentin in Patients with Spinal Cord Injury-Related Neuropathic Pain: A Network Meta-Analysis.

Pain Ther 2021 Sep 7. Epub 2021 Sep 7.

Department of Neurology, Gongren Hospital, Tangshan, 063000, Hebei, China.

Introduction: This study was performed to explore the efficacy and safety of pregabalin and gabapentin in patients with spinal cord injury (SCI)-induced neuropathic pain to determine which treatment is most suitable for such patients.

Methods: We searched the PubMed, MEDLINE, Embase, and Cochrane Library databases from database inception to August 31, 2020. The quality of the included studies was assessed. We selected the average pain intensity after treatment and the proportion of patients who discontinued treatment because of adverse effects as the outcome indicators for efficacy and safety, respectively. Statistical analyses were performed using Stata, v16.0, and RevMan, v5.3, software.

Results: We included eight randomized controlled trials that examined four interventions (pregabalin, gabapentin, carbamazepine, and amitriptyline). Based on the average pain intensity after treatment, the efficacy order from highest to lowest was pregabalin, gabapentin, amitriptyline, carbamazepine, and placebo. Based on the proportion of patients who discontinued treatment because of adverse effects, the order from highest to lowest was pregabalin, amitriptyline, carbamazepine, gabapentin, and placebo. In addition, five studies reported the overall incidence of treatment-related adverse effects for two interventions (pregabalin and gabapentin). According to the pooled analysis of these studies, the order for the overall incidence of treatment-related adverse effects from highest to lowest was pregabalin, gabapentin, and placebo.

Conclusions: This study revealed that for patients with SCI-related neuropathic pain, pregabalin was the most effective for relieving pain, whereas gabapentin performed better in aspects associated with drug therapy-related safety.
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http://dx.doi.org/10.1007/s40122-021-00302-8DOI Listing
September 2021

Suppression of Sunscreen Leakage in Water by Amyloid-like Protein Aggregates.

ACS Appl Mater Interfaces 2021 Sep 4;13(36):42451-42460. Epub 2021 Sep 4.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.

A sunscreen offers indispensable skin protection against UV damage and related skin diseases. However, due to the poor interfacial stability of sunscreen coatings on the skin, the synthetic ingredients in sunscreen creams easily fall off and enter aquatic environments, causing large ecological hazards and skin protection failure. Herein, we tackle this issue by introducing amyloid-like protein aggregates into a sunscreen to noticeably enhance the interfacial robustness of sunscreen coatings on the skin. The synthesis of such an agent to suppress sunscreen leakage can be achieved by manipulating the phase transition of bovine serum albumin (BSA) in a mild aqueous solution at room temperature. The resulting phase-transitioned BSA (PTB) aggregates effectively entrap the sunscreen ingredients to generate a uniform cream coating on the skin with robust amyloid-mediated interfacial adhesion stability. With continuous flushing in aquatic environments, such as salt water and seawater, this PTB-modified sunscreen (PTB sunscreen) coated on the skin maintains a retention ratio as high as >92%, which is 2-10 times higher than those of commercially available sunscreen products. The high retention ratio of the PTB sunscreen in aquatic environments demonstrates the great potential of amyloid-like protein aggregates in the development of leakage-free sunscreens with low ecosystem hazards and long-lasting UV protection in aquatic environments.
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http://dx.doi.org/10.1021/acsami.1c11307DOI Listing
September 2021

Novel Enhanced Lanthanide Electrochemiluminescence Luminophores: Ce-Doped TbPO Facile Synthesis and Detection for Mucin1.

Anal Chem 2021 09 27;93(36):12289-12295. Epub 2021 Aug 27.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

Despite the upsurging interest in electrochemiluminescence (ECL) of lanthanides, the research in this field is still in its infancy due to the low intensity. In this work, a series of Ce-doped terbium orthophosphates (TbPO:Ce) in different proportions have been synthesized through the co-precipitation method at room temperature. Meanwhile, through the investigation of morphologies and ECL properties of these TbPO:Ce, it is concluded that the ECL intensity reaches the maximum when the molar ratio of Tb/Ce is 9:1 and the material is nanorod-shaped. The ECL intensity of TbPO:Ce is significantly improved by doping with Ce due to the dual sensitization strategy of the antenna effect from PO to Tb and the energy transfer from Ce to Tb. Interestingly, doping with Ce can not only adjust morphology of TbPO:Ce but also improve the ECL intensity. In addition, to verify the application of TbPO:Ce, two single mucin1 (MUC1) aptamers are linked together to form a dual MUC1 aptamer chain. Then, a simple and sensitive ECL biosensor is constructed for the detection of MUC1, which can recognize the double amount of MUC1 and quench the ECL signal. As expected, the proposed biosensor shows good stability and acceptable selectivity and achieves sensitive detection of MUC1 with a dynamic range from 1 fg·mL to 10 ng·mL and a limit of detection of 0.5 fg·mL. This work may pave a new avenue for the study of direct ECL emission of lanthanides and prove to be ideal for the research of new ECL luminophores in electrochemical analysis.
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http://dx.doi.org/10.1021/acs.analchem.1c01916DOI Listing
September 2021

Elevated LINC00909 Promotes Tumor Progression of Ovarian Cancer via Regulating the miR-23b-3p/MRC2 Axis.

Oxid Med Cell Longev 2021 21;2021:5574130. Epub 2021 Jul 21.

Department of Gynecological Oncology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, No. 145 Middle Shandong Road, Huangpu District, Shanghai 200001, China.

Ovarian cancer (OC), the third common gynecologic malignancy, contributes to the most cancer-caused mortality in women. However, 70% of patients with OC are diagnosed at an advanced stage, of which the 5-year survival is less than 30%. Long noncoding RNAs (long ncRNAs or lncRNA), a type of RNA with exceeding 200 nucleotides in length but no protein-coding capability, have been demonstrated to involve the pathogenesis of various cancers and show considerable potential in the diagnosis of OC. In this study, we found that the LINC00909 expression in tumor and serum specimens of OC patients was elevated, determined by real-time quantitative, and droplet digital PCR. In receiver operating characteristic (ROC) analysis, our results revealed that serum LINC00909 distinguished cancers from normal ovarian tissue with 87.8% of sensitivity and 69.6% of specificity (AUC, 81.2%) and distinguished serous ovarian cancer from normal ovarian tissue with 90.0% of sensitivity and 75.9% of specificity (AUC, 84.5%). Furthermore, we observed that the tumor and serum LINC00909 level was positively associated with the International Federation of Gynecology and Obstetrics (FIGO) stage and the Eastern Cooperative Oncology Group (ECOG) score (reflecting patients' performance status). Also, patients with low serum LINC00909 level showed a longer overall (hazard ratio, HR = 1.874, = 0.0004) and progression-free (HR = 1.656, = 0.0017) survival. Functional assays indicated that the elevation of LINC00909 expression contributes to cell proliferation, migration, and invasion capability of ovarian cancer cells. Besides, we demonstrated that LINC00909 functions as a competing endogenous RNA (ceRNA) of MRC2 mRNA by sponging miR-23-3p, and thereby promotes epithelial-to-mesenchymal transition (EMT) of ovarian cancer cells. Therefore, we highlight that the LINC00909/miR-23b-3p/MRC2 axis is implicated in the pathogenesis of ovarian cancer, and serum LINC00909 may be a promising biomarker for the diagnosis of OC.
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http://dx.doi.org/10.1155/2021/5574130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318762PMC
July 2021

The Clinical Characteristics of ARDS in Children With Hematological Neoplasms.

Front Pediatr 2021 8;9:696594. Epub 2021 Jul 8.

Department of Intensive Care Medicine, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.

In order to explore the clinical characteristics of pediatric patients admitted to the pediatric intensive care unit (PICU) who suffered from hematological neoplasms complicated with acute respiratory distress syndrome (ARDS), we retrospectively analyzed 45 ARDS children with hematological neoplasms who were admitted to the PICU of Shanghai Children's Medical Center from January 1, 2014, to December 31, 2020. The 45 children were divided into a survival group and a non-survival group, a pulmonary ARDS group and an exogenous pulmonary ARDS group, and an agranulocytosis group and a non-agranulocytosis group, for statistical analysis. The main clinical manifestations were fever, cough, progressive dyspnea, and hypoxemia; 55.6% (25/45) of the children had multiple organ dysfunction syndrome (MODS). The overall mortality rate was 55.6% (25/45). The vasoactive inotropic score (VIS), pediatric critical illness scoring (PCIS), average fluid volume in the first 3 days and the first 7 days, and the incidence of MODS in the non-survival group were all significantly higher than those in the survival group ( < 0.05). However, total length of mechanical ventilation and length of hospital stay and PICU days in the non-survival group were significantly lower than those in the survival group ( < 0.05). The PCIS (OR = 0.832, = 0.004) and the average fluid volume in the first 3 days (OR = 1.092, = 0.025) were independent risk factors for predicting death. Children with exogenous pulmonary ARDS were more likely to have MODS than pulmonary ARDS ( < 0.05). The mean values of VIS, C-reactive protein (CRP), and procalcitonin (PCT) in children with exogenous pulmonary ARDS were also higher ( < 0.05). After multivariate analysis, PCT was independently related to exogenous pulmonary ARDS. The total length of hospital stay, peak inspiratory pressure (PIP), VIS, CRP, and PCT in the agranulocytosis group were significantly higher than those in the non-agranulocytosis group ( < 0.05). Last, CRP and PIP were independently related to agranulocytosis. In conclusion, children with hematological neoplasms complicated with ARDS had a high overall mortality and poor prognosis. Children complicated with MODS, positive fluid balance, and high VIS and PCIS scores were positively correlated with mortality. In particular, PCIS score and average fluid volume in the first 3 days were independent risk factors for predicting death. Children with exogenous pulmonary ARDS and children with agranulocytosis were in a severely infected status and more critically ill.
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http://dx.doi.org/10.3389/fped.2021.696594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295493PMC
July 2021

Metformin Antagonizes Ovarian Cancer Cells Malignancy Through MSLN Mediated IL-6/STAT3 Signaling.

Cell Transplant 2021 Jan-Dec;30:9636897211027819

Department of Obstetrics and Gynecology, The Fifth Affiliated People's Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, People's Republic of China.

Background: Ovarian cancer is the most lethal gynecological malignancy, and chemotherapy remains the cornerstone for ovarian cancer management. Due to the unsatisfactory prognosis, a better understanding of the underlying molecular carcinogenesis is urgently required.

Methods: Assays for determining cell growth, cell motility, and apoptosis were employed to evaluate the potential antitumor effects of metformin against ovarian cancer cells. Molecular biological methods were employed to explore the underlying mechanism. Human ovarian cancer samples and Gene Expression Profiling Interactive Analysis (GEPIA) dataset were used for uncovering the clinical significances of mesothelin (MSLN) on ovarian cancer.

Results: In the present work, we found that metformin treatment led to cell growth and cell migration inhibition, and induced cell apoptosis. Metformin administration also impaired cancer cell stemness and the capillary-like structure formation capacity of SKOV3 cells. On mechanism, metformin treatment remarkably reduced mesothelin (MSLN) expression, downregulated IL-6/STAT3 signaling activity, subsequently resulted in VEGF and TGFβ1 expression. We also observed an oncogenic function of MSLN on ovarian cancer.

Conclusions: Collectively, our findings suggested that metformin exerts anticancer effects by suppressing ovarian cancer cell malignancy, which attributed to MSLN inhibition mediated IL6/STAT3 signaling and VEGF and TGFβ1 downregulation.
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http://dx.doi.org/10.1177/09636897211027819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274104PMC
July 2021

Structure and functions of bacterial outer membrane protein A, a potential therapeutic target for bacterial infection.

Curr Top Med Chem 2021 07 5. Epub 2021 Jul 5.

Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228, China.

Outer membrane protein A (OmpA) is a unique outer membrane protein which is abundantly present in the outer membrane of Gram-negative bacteria. OmpA is a transmembrane structural protein with a conserved amino acid sequence among different bacteria. This protein is involved in a number of functions like adhesion, toxicity, invasiveness, and biofilm formation in Gram-negative bacteria. Many studies have proposed that OmpA could be a therapeutic target for bacterial infection. Our review focusses on the studies involving recent development in the structure and functions of OmpA and further discussing its potential as a therapeutic target for bacterial infection.
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http://dx.doi.org/10.2174/1568026621666210705164319DOI Listing
July 2021

The Application of Transbronchial Lung Cryobiopsy and Uniportal and Tubeless Video-Assisted Thoracic Surgery in the Multidisciplinary Diagnosis of Interstitial Lung disease-A Real-World Prospective Study.

Front Mol Biosci 2021 16;8:681669. Epub 2021 Jun 16.

Department of Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

The application of transbronchial lung cryobiopsy (TBLC) and uniportal and tubeless video-assisted thoracic surgery (UT-VATS) in the multidisciplinary diagnosis of interstitial lung disease (ILD) has not been demonstrated in real-world clinical practice. This prospective study included 137 patients with no definitive diagnosis who were the subject of two multidisciplinary discussion (MDD) sessions. As indicated in the first MDD, 67 patients underwent UT-VATS and 70 underwent TBLC. The specificity of biopsy information and its contribution to final MDD diagnosis were evaluated in the second MDD. The post-operative complications and hospitalization costs associated with the two biopsy methods were compared. UT-VATS was favored for patients initially diagnosed with idiopathic pulmonary fibrosis (IPF), bronchiolitis-associated interstitial lung disease (RB-ILD)/desquamative interstitial pneumonia (DIP) and undefined idiopathic interstitial pneumonia (UIIP), while TBLC was preferred for pulmonary lymphangioleiomyomatosis (PLAM) and pulmonary alveolar proteinosis (PAP). The spirometry parameters were better in patients who underwent UT-VATS than those who underwent TBLC. UT-VATS provided more specific pathological results than TBLC (85.7 vs 73.7%, = 0.06). In patients initially diagnosed with UIIP, pathological information from UT-VATS was more clinically useful than that obtained from TBLC, although both tests contributed similarly to cases initially diagnosed as interstitial pneumonia with auto-immune features (IPAF)/connective tissue disease-related ILD (CTD-ILD). The safety of UT-VATS was comparable with TBLC although TBLC was cheaper during hospitalization (US$4,855.7 vs US$3,590.9, < 0.001). multidisciplinary discussion decisions about biopsies were driven by current knowledge of sampling and diagnosis capacity as well as potential risks of different biopsy methods. The current MDD considered UT-VATS more informative than TBLC in cases initially diagnosed as UIIP although they were equally valuable in patients initially diagnosed with IPAF/CTD-ILD.
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http://dx.doi.org/10.3389/fmolb.2021.681669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241905PMC
June 2021

Single-cell RNA-seq revealed diverse cell types in the mouse placenta at mid-gestation.

Exp Cell Res 2021 Aug 2;405(2):112715. Epub 2021 Jul 2.

Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, Xiamen, China. Electronic address:

The mammalian placenta consists of a set of cells to ensure normal placental functions throughout gestation. Dysfunctional placentae are considered as the origin of a series of pregnancy complications. Therefore, it is urgent for detailed information about the molecular recipes of the cell types within the normal placenta. In the past years, gene expression analysis via single-cell RNA-seq (scRNA-seq) offers opportunities to identify new cell types in a variety of organs and tissues. In this study, scRNA-seq was used to explore the cell heterogeneity within the E10.5 mouse placenta and unravel their discrepancies in cell composition and communications. We identified sixteen cell clusters, including some cell clusters that originated from the maternal tissue. Moreover, we traced the developmental trajectories of trophoblasts and Hofbauer-like cells. Further analysis revealed cell connections between the endothelial cells and pericytes, syncytiotrophoblasts, as well as decidual cells. Besides, we highlighted several signaling pathways, such as the EGF, FGF, canonical, and non-canonical WNT signaling pathways, which mediated the potential crosstalk between different cell types within placenta. Our research provides an in-depth understanding of placental development, cellular composition, and communications at the maternal-fetal interface.
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http://dx.doi.org/10.1016/j.yexcr.2021.112715DOI Listing
August 2021

Three new species of the subgenus Jaynesia Allen, 1969 of the genus Tiphia Fabricius, 1775 (Hymenoptera: Tiphiidae: Tiphiinae) from China, with a key to all known species.

Zootaxa 2021 May 14;4970(2):313324. Epub 2021 May 14.

Chongqing Key Laboratory of Vector Insects; Chongqing Key Laboratory of Animal Biology; Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China.

In this study, three new species of the subgenus Jaynesia Allen from China are described and illustrated, namely T. hohehotensis sp. nov. from Inner Mongolia, T. displicata sp. nov. from Sichuan, and T. rotunda sp. nov. from Gansu. A key to all known species of Jaynesia is also provided.
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http://dx.doi.org/10.11646/zootaxa.4970.2.5DOI Listing
May 2021

Hydrogen gas post-conditioning attenuates early neuronal pyroptosis in a rat model of subarachnoid hemorrhage through the mitoK signaling pathway.

Exp Ther Med 2021 Aug 4;22(2):836. Epub 2021 Jun 4.

Department of Radioactive Intervention, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.

Neuronal pyroptosis serves an important role in the progress of neurologic dysfunction following subarachnoid hemorrhage (SAH), which is predominantly caused by a ruptured aneurysm. Hydrogen gas has been previously reported to be an effective anti-inflammatory agent against ischemia-associated diseases by regulating mitochondrial function. The objective of the present study was to investigate the potential neuroprotective effects of hydrogen gas post-conditioning against neuronal pyroptosis after SAH, with specific focus on the mitochondrial ATP-sensitive K (mitoK) channels. Following SAH induction by endovascular perforation, rats were treated with inhalation of 2.9% hydrogen gas for 2 h post-perforation. Neurologic deficits, brain water content, reactive oxygen species (ROS) levels, neuronal pyroptosis, phosphorylation of ERK1/2, p38 MAPK and pyroptosis-associated proteins IL-1β and IL-18 were evaluated 24 h after perforation by a modified Garcia method, ratio of wet/dry weight, 2',7'-dichlorofluorescin diacetate, immunofluorescence and western blot assays, respectively. An inhibitor of the mitoK channel, 5-hydroxydecanoate sodium (5-HD), was used to assess the potential role of the mitoK-ERK1/2-p38 MAPK signal pathway. Hydrogen gas post-conditioning significantly alleviated brain edema and improved neurologic function, reduced ROS production and neuronal pyroptosis, suppressed the expression of IL-1β and IL-18 whilst upregulating ERK1/2 phosphorylation, but downregulated p38 MAPK activation 24 h post-SAH. These aforementioned effects neuroprotective were partially reversed by 5-HD treatment. Therefore, these observations suggest that post-conditioning with hydrogen gas ameliorated SAH-induced neuronal pyroptosis at least in part through the mitoK/ERK1/2/p38 MAPK signaling pathway.
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http://dx.doi.org/10.3892/etm.2021.10268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200808PMC
August 2021

Functionalized europium-porphyrin coordination polymer: Rational design of high performance electrochemiluminescence emitter for mucin 1 sensing.

Biosens Bioelectron 2021 Nov 9;191:113422. Epub 2021 Jun 9.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China. Electronic address:

The excellent characteristics of porphyrins have inspired widespread interest in electrochemiluminescence (ECL). However, the limited ECL intensity and poor stability of porphyrins in aqueous solution are still severely restricted further biological application. Here, we subtly synthesized a functionalized europium and 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (TCPP) coordination polymer (Eu-PCP) by a one-step solvothermal method. In sharp contrast to the pristine TCPP, Eu-PCP showed a higher and more stable ECL red-light emission (673 nm) at low potential (-1.1 V, vs Ag/AgCl), which was 7.7-fold higher ECL intensity and 4.6-fold efficiency. In view of the crucial role of mucin 1 (MUC1) in tumor overexpression, it was selected as the target molecule. Combined with exonuclease III (Exo III)-assisted recycling amplification strategy, a ternary ECL biosensor was constructed for the MUC1 detection based on Eu-PCP as a satisfied ECL emitter, gold nanoparticles capped CeO ([email protected]) as the coreactant accelerator and peroxydisulfate as coreactant. Meanwhile, gold nanoparticles capped MnO ([email protected]) was used as the quenching probe to achieve a highly sensitive detection of MUC1. The proposed biosensor exhibited a wide linear range from 1 fg mL to 10 ng mL with a low limit of detection (0.32 fg mL). By changing the corresponding target recognition DNA, this strategy could be expanded to detect other biomarkers.
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http://dx.doi.org/10.1016/j.bios.2021.113422DOI Listing
November 2021

Extensive serum biomarker analysis in the prethrombotic state of recurrent spontaneous abortion.

J Cell Mol Med 2021 Jul 16;25(14):6679-6694. Epub 2021 Jun 16.

Department of Traditional Chinese Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

The prethrombotic state (PTS) is a possible cause of recurrent spontaneous abortion (RSA). The aim of this study was to identify serum biomarkers for the detection of RSA with PTS (PSRSA). A Quantibody array 440 was used to screen novel serum-based biomarkers for PSRSA/NRSA (RSA without PTS). Proteins differentially expressed in PSRSA were analysed using bioinformatics methods and subjected to a customized array and enzyme-linked immunosorbent assay (ELISA) validation. We used receiver operating characteristic to calculate diagnostic accuracy, and machine learning methods to establish a biomarker model for evaluation of the identified targets. 20 targets were selected for validation using a customized array, and seven targets via ELISA. The decision tree model showed that IL-24 was the first node and eotaxin-3 was the second node distinguishing the PSRSA and NRSA groups (an accuracy rate of 100% and an AUC of 1). Epidermal growth factor (EGF) as the node distinguished the PSRSA and NC groups (an accuracy rate of 100% and an AUC of 1). EGF as the node distinguished the NRSA and NC groups (an accuracy rate of 96.5% and an AUC of 0.998). Serum DNAM-1, BAFF, CNTF, LAG-3, IL-24, Eotaxin-3 and EGF represent a panel of promising diagnostic biomarkers to detect the PSRSA.
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http://dx.doi.org/10.1111/jcmm.16671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278118PMC
July 2021

MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm.

J Cell Mol Med 2021 Jun 15. Epub 2021 Jun 15.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Vascular smooth muscle cells (VSMCs) senescence contributes to abdominal aortic aneurysm (AAA) formation although the underlying mechanisms remain unclear. This study aimed to investigate the role of miR-199a-5p in regulating VSMC senescence in AAA. VSMC senescence was determined by a senescence-associated β-galactosidase (SA-β-gal) assay. RT-PCR and Western blotting were performed to measure miRNA and protein level, respectively. The generation of reactive oxygen species (ROS) was evaluated by H2DCFDA staining. Dual-luciferase reporter assay was used to validate the target gene of miR-199a-5p. VSMCs exhibited increased senescence in AAA tissue relative to healthy aortic tissue from control donors. Compared with VSMCs isolated from control donors (control-VSMCs), those derived from patients with AAA (AAA-VSMCs) exhibited increased cellular senescence and ROS production. Angiotensin II (Ang II) induced VSMC senescence by promoting ROS generation. The level of miR-199a-5p expression was upregulated in the plasma from AAA patients and Ang II-treated VSMCs. Mechanistically, Ang II treatment significantly elevated miR-199a-5p level, thereby stimulating ROS generation by repressing Sirt1 and consequent VSMC senescence. Nevertheless, Ang II-induced VSMC senescence was partially attenuated by a miR-199a-5p inhibitor or Sirt1 activator. Our study revealed that miR-199a-5p aggravates Ang II-induced VSMC senescence by targeting Sirt1 and that miR-199a-5p is a potential therapeutic target for AAA.
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http://dx.doi.org/10.1111/jcmm.16485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366448PMC
June 2021

Effects of music stimulus on behavior response, cortisol level, and horizontal immunity of growing pigs.

J Anim Sci 2021 May;99(5)

Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.

An enriched environment is widely used to improve domestic animals' welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs.
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http://dx.doi.org/10.1093/jas/skab043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092926PMC
May 2021

Synergistic enhancement of tendon-to-bone healing via anti-inflammatory and pro-differentiation effects caused by sustained release of Mg/curcumin from injectable self-healing hydrogels.

Theranostics 2021 3;11(12):5911-5925. Epub 2021 Apr 3.

Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.

Poor healing response after rotator cuff reconstruction is multifactorial, with the inflammatory microenvironment and deficiency of stem cell differentiation factors at the lesion site being most relevant. However, there is a lack of effective tissue engineering strategies that can simultaneously exert anti-inflammatory and pro-differentiation effects to promote rotator cuff healing. In this study, we synthesized and characterized a novel active drug delivery vector that successfully overcame the challenge of simultaneous high-efficiency loading and controlled release of Mg and curcumin. The anti-inflammatory and pro-differentiation effects of the composite hydrogel were evaluated and . Moreover, healing of the rotator cuff tendon-to-bone interface was studied by histology, immunofluorescence, and biomechanical tests. The composite hydrogel exhibited excellent biocompatibility and injectability, good adhesiveness, and rapid self-healing. The released curcumin showed obvious anti-inflammatory and antioxidation effects, which protected stem cells and tendon matrix. Furthermore, released Mg promoted stem cell aggregation and chondrogenesis. Moreover, biomechanical tests and histological results of a rat rotator cuff tear model at 8 weeks after surgery indicated that the composite hydrogel significantly enhanced tendon-to-bone healing. The composite hydrogel mediated sustained release of curcumin and Mg to effectively promote rotator cuff tendon-to-bone healing via anti-inflammatory and pro-differentiation effects. Therefore, this composite hydrogel offers significant promise for rotator cuff repair.
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http://dx.doi.org/10.7150/thno.56266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058719PMC
July 2021

Management and Prognosis of Interstitial Lung Disease With Lung Cancer (ILD-LC): A Real-World Cohort From Three Medical Centers in China.

Front Mol Biosci 2021 31;8:660800. Epub 2021 Mar 31.

State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of the Respiratory Health, Guangzhou Medical University, Guangzhou, China.

Background And Objective: Interstitial lung disease with lung cancer (ILD-LC) is rare and its management has not been fully described. This study aimed to investigate the management and prognosis of ILD-LC patients in China.

Methods: The present analysis is a retrospective real-world cohort study. Clinical data of ILD-LC patients were obtained from 3 hospitals in China. The overall survival (OS) of patients was analyzed. Univariate and multivariate regression analyses were performed.

Results: One hundred eighty-four ILD-LC patients included were biased toward male (85.3%), smokers (75.5%), idiopathic pulmonary fibrosis (IPF) (58.2%) patients with comorbidities (67.9%) and ECOG-PS score of 1 (65.2%). Most patients were advanced peripheral non-small cell lung cancer. The initial anti-cancer regimen for ILD-LC is mainly chemotherapy, and patients with early-stage LC prefer surgery. In the anti-cancer cohort, the number of ILD-LC patients who underwent the 2nd and 3rd or more anti-cancer regimens were 78 (55.7%) and 32 (22.8%), respectively. In the non-anticancer cohort, the median OS was 3.5 months. In the early-stage cohort, the median OS was 14.2 months in the systematic therapy group; however, the median OS was not reached in the surgery group. In the advanced-stage cohort with systematic therapy, the median OS was 7.2 months. Interstitial pneumonia (IIP) and anti-angiogenesis were associated with OS in the univariate analysis, whereas anti-angiogenesis was an independent protective factor for advanced LC with ILD.

Conclusion: Patients with ILD-LC have very poor prognosis. Appropriate anti-tumor treatment can prolong the survival time of patients who can tolerate it. Targeted therapy and immunotherapy are alternative treatments for LC patients with mild ILD. For ILD patients with advanced LC, antiangiogenic regimens significantly improve the prognosis of the disease.
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http://dx.doi.org/10.3389/fmolb.2021.660800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044367PMC
March 2021

Forced vital capacity predicts the survival of interstitial lung disease in anti-MDA5 positive dermatomyositis: a multi-centre cohort study.

Rheumatology (Oxford) 2021 Mar 25. Epub 2021 Mar 25.

Department of Rheumatology, Renji Hospital South Campus, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: Anti-melanoma differentiation-associated gene 5 (MDA5) positive dermatomyositis (DM) is a life-threatening disease often complicated with rapidly progressive interstitial lung disease (ILD). This study aimed to establish and validate a clinical prediction model for 6-month all-cause mortality in Chinese patients with anti-MDA5 positive DM-ILD.

Methods: We conducted a retrospective observational study using a single-centre derivation cohort and a multi-centre validation cohort. Hospitalized DM patients with positive anti-MDA5 antibody and ILD course ≤3 months on admission were included. Patients' baseline characteristics were described and compared between the deceased and survivors by univariable Cox regression. Optimal cut-off values were defined by the 'survminer' R package for significant continuous variables. Independent prognostic factors were determined by the final multivariable Cox regression model chosen by backward stepwise algorithm, which could be reproduced in both cohorts. The Kaplan-Meier survival analyses based on the derived predictor were conducted.

Results: A total of 184 and 81 eligible patients were included with a cumulative 40.8% and 40.7% six-month mortality in the derivation and validation cohorts, respectively. Based on multivariable Cox regression, the prognostic factor at baseline was identified and validated as three-category forced vital capacity (FVC)%: FVC% ≥ 50%, FVC% <50%, unable to perform. This significantly distinguishes three risk stages with mortalities of 15.3%, 46.8%, 97.4% in the derivation cohort, and 14.9%, 58.3%, 86.4% in the validation cohort, respectively (all p < 0.05).

Conclusion: The validated FVC%-based categorical predictor in anti-MDA5 positive DM-ILD is helpful for risk stratification in clinical practice and might facilitate cohort enrichment for future trials.
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http://dx.doi.org/10.1093/rheumatology/keab305DOI Listing
March 2021

Is there possibility of vertical transmission of COVID-19: a systematic review.

Transl Pediatr 2021 Feb;10(2):423-434

Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

In order to investigate the clinical features of pregnant women and their neonates with coronavirus disease 2019 (COVID-19) and the evidence of vertical transmission of COVID-19, we retrieved studies included in PubMed, Medline and Chinese databases from January 1, 2000 to October 25, 2020 using relevant terms, such as 'COVID-19', 'vertical transmission' . in 'Title/Abstract'. Case reports and case series were included according to the inclusion and exclusion criteria. We conducted literature screening and data extraction, and performed literature bias risk assessment. Total of 13 case series and 16 case reports were collected, including a total of 564 pregnant women with COVID-19 and their 555 neonates, of which 549 neonates received nucleic acid test for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and 18 neonates was diagnosed with COVID-19. The positive rate is 3.28%. Amniotic fluid of one woman was tested positive for SARS-CoV-2. The majority of infected neonates were born under strict infection control and received isolation and artificial feeding. Up till now, there is no sufficient evidence to exclude the possibility of vertical transmission for COVID-19 based on the current available data.
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http://dx.doi.org/10.21037/tp-20-144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944168PMC
February 2021

Determination of ascorbic acid using electrochemiluminescence sensor based on nitrogen and sulfur doping graphene quantum dots with luminol as internal standard.

Mikrochim Acta 2021 03 10;188(4):120. Epub 2021 Mar 10.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.

A novel internal standard electrochemiluminescence (ECL) sensor has been designed for the detection of ascorbic acid (AA). The adopted dual-emission luminophore (NSGQDs-PEI-luminol-Pt) is composed of nitrogen and sulfur double-doped graphene quantum dots (NSGQDs, as the main luminophore), luminol (as the auxiliary luminophore and internal standard), platinum nanoparticles (Pt NPs, as the co-reaction accelerator), and polyetherimide (PEI, as the linker of NSGQDs and luminol). The results suggest obviously enhanced  ECL intensities by the Förster resonance energy transfer (FRET) between luminol (donor) and NSGQDs (acceptor). In this sensing system, the cathodic ECL intensities of NSGQDs (ECL-1, -1.8 V vs. Ag/AgCl) gradually decrease with increasing concentration of AA, while the anodic ECL intensities of luminol (ECL-2, 0.3 V vs. Ag/AgCl) almost remain essentially constant at a potential window from -2.0 to 0.4 V. The natural logarithm of the ratio between ECL-1 and ECL-2 (ln I ) shows a good linear relationship with AA concentration ranging from 10 to 360 nM. The regression equation is ln I  = - 0.0059 c + 3.55 (R = 0.992) with a limit of detection of 3.3 nM. Such sensor has also been applied for monitoring AA in human serum. The recovery range was 96.5-105.3% and the relative standard deviation was  1.3-3.3%.
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http://dx.doi.org/10.1007/s00604-021-04761-wDOI Listing
March 2021

An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases.

Parasitol Res 2021 May 2;120(5):1799-1809. Epub 2021 Mar 2.

Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, Hainan, China.

Present-day diagnostic tools and technologies for canine diseases and other vector-borne parasitic diseases hardly meet the requirements of an efficient and rapid diagnostic tool, which can be suitable for use at the point-of-care in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been always a method of choice in the development and validation of quick, precise, and sensitive diagnostic assays for pathogen detection and to reorganize point-of-care (POC) molecular diagnostics. In this study, we have demonstrated an efficient detection system for parasitic vector-borne pathogens like Ehrlichia canis and Hepatozoon canis by linking the LAMP assay to a smartphone via a simple, inexpensive, and a portable "LAMP box," All the components of the LAMP box were connected to each other wirelessly. This LAMP box was made up of an isothermal heating pad mounted below an aluminum base which served as a platform for the reaction tubes and LAMP assay. The entire setup could be connected to a smartphone via an inbuilt Wi-Fi that allowed the user to establish the connection to control the LAMP box. A 5 V USB power source was used as a power supply. The sensitivity of the LAMP assay was estimated to be up to 10 dilution limit using the amplified, purified, and quantified specific DNA templates. It can also serve as an efficient diagnostic platform for many other veterinary infectious or parasitic diseases of zoonotic origin majorly towards field-based diagnostics.
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http://dx.doi.org/10.1007/s00436-021-07077-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920752PMC
May 2021

Inhibition of miR-199a-5p rejuvenates aged mesenchymal stem cells derived from patients with idiopathic pulmonary fibrosis and improves their therapeutic efficacy in experimental pulmonary fibrosis.

Stem Cell Res Ther 2021 02 25;12(1):147. Epub 2021 Feb 25.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510080, Guangdong, China.

Background: Idiopathic pulmonary fibrosis (IPF) is an age-related disease with no cure. Mesenchymal stem cell (MSC)-based therapy has emerged as a novel strategy for IPF treatment. Nevertheless, MSCs derived from patients with IPF (IPF-MSCs) become senescent, thereby reducing their beneficial effects in IPF. MicroRNAs (miRNAs) mediate the senescence of MSCs, but the underlying mechanisms are not fully understood. We investigated the mechanisms by which miR-199a-5p regulates IPF-MSC senescence and whether its inhibition could rejuvenate IPF-MSCs and enhance their therapeutic efficacy.

Methods: Control-MSCs and IPF-MSCs were isolated from the adipose tissue of age-matched healthy and IPF donors, respectively. Cell senescence was examined by senescence-associated β-galactosidase (SA-β-gal) staining. The level of miR-199a-5p was measured by RT-PCR. Autophagy was determined using a transmission electron microscope (TEM). The therapeutic efficacy of anti-miR-199a-5p-IPF-MSCs was assessed using a mouse model of bleomycin-induced lung fibrosis.

Results: Despite similar surface makers, IPF-MSCs exhibited increased cellular senescence and decreased proliferative capacity compared with control-MSCs. The expression of miR-199a-5p was significantly enhanced in the serum of IPF patients and IPF-MSCs compared with that of healthy donors and control-MSCs. The upregulation of miR-199a-5p induced senescence of control-MSCs, whereas the downregulation rescued IPF-MSC senescence. Mechanistically, miR-155-5p suppressed autophagy of MSCs via the AMPK signaling pathway by downregulating the expression of Sirtuin 1(Sirt1), resulting in cellular senescence. Accordingly, miR-155-5p inhibition promoted autophagy and ameliorated IPF-MSC senescence by activating the Sirt1/AMPK signaling pathway. Compared with IPF-MSCs, the transplantation of anti-miR-199a-5p-IPF-MSCs increased the ability to prevent progression of pulmonary fibrosis in bleomycin-treated mice.

Conclusions: Our study shows that miR-199a-5p regulates MSC senescence in patients with IPF by regulating the Sirt1/AMPK signaling pathway and miR-199a-5p is a novel target to rejuvenate IPF-MSCs and enhance their beneficial effects.
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http://dx.doi.org/10.1186/s13287-021-02215-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905557PMC
February 2021

Development and validation of a 30-day death nomogram in patients with spontaneous cerebral hemorrhage: a retrospective cohort study.

Acta Neurol Belg 2021 Feb 10. Epub 2021 Feb 10.

Department of Neurosurgery, North China University of Science and Technology Affiliated Hospital, Tangshan, 063000, Hebei, China.

The purpose of this study was to establish and validate a nomogram to estimate the 30-day probability of death in patients with spontaneous cerebral hemorrhage. From January 2015 to December 2017, a cohort of 450 patients with clinically diagnosed cerebral hemorrhage was collected for model development. The minimum absolute contraction and the selection operator (lasso) regression model were used to select the strongest prediction of patients with cerebral hemorrhage. Discrimination and calibration were used to evaluate the performance of the resulting nomogram. After internal validation, the nomogram was further assessed in a different cohort containing 148 consecutive subjects examined between January 2018 and December 2018. The nomogram included five predictors from the lasso regression analysis, including: Glasgow coma scale (GCS), hematoma location, hematoma volume, white blood cells, and D-dimer. Internal verification showed that the model had good discrimination, (the area under the curve is 0.955), and good calibration [unreliability (U) statistic, p = 0.739]. The nomogram still showed good discrimination (area under the curve = 0.888) and good calibration [U statistic, p = 0.926] in the verification cohort data. Decision curve analysis showed that the prediction nomogram was clinically useful. The current study delineates a predictive nomogram combining clinical and imaging features, which can help identify patients who may die of cerebral hemorrhage.
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http://dx.doi.org/10.1007/s13760-021-01617-1DOI Listing
February 2021
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