Publications by authors named "Qian Ding"

271 Publications

Recent advances in cell membrane-camouflaged nanoparticles for inflammation therapy.

Drug Deliv 2021 Dec;28(1):1109-1119

Department of Pharmacy, the Affiliated Hospital of Southwest Medical University, Luzhou, China.

During inflammation, inflammatory cells are rapidly recruited to sites of infection or injury, where they cross physiological barriers around the infected site and further infiltrate into the tissues. Other cells, such as erythrocytes, endothelial cells and stem cells, also play prominent roles in host defense and tissue repair. In recent years, nanotechnology has been exploited to deliver drugs to sites of inflammation. For example, nanoparticles camouflaged with a cell membrane are a novel drug-delivery platform that can interact with the immune system and that show great potential for treating inflammation. Encapsulating drugs inside plasma membranes derived from various cells involved in inflammatory processes can be effective against inflammation. This review describes the preparation, characterization, and properties of various types of cell membrane-camouflaged biomimetic nanoparticles. It also summarizes preclinical research into their efficacy against inflammation.
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http://dx.doi.org/10.1080/10717544.2021.1934188DOI Listing
December 2021

Dynamic Changes of Arc Expression in Dorsal Striatum of Mice After Self-Administration of Sucrose.

Front Cell Neurosci 2021 19;15:654521. Epub 2021 May 19.

Department of Rehabilitation Medicine, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.

Region-specific plasticity in the striatal circuit plays an important role in the development and long-term maintenance of skills and sequential movement procedures. Studies investigating the molecular substrates that contribute to the plasticity changes during motor skill processes have documented a transition in expression from the dorsomedial striatum (DMS) to the dorsolateral striatum (DLS); however, few studies have explored the expression pattern of molecular substrates in the dorsal striatum during progression of instrumental learning. To address this issue, the activity-regulated cytoskeleton-associated protein (Arc) expressions in the subregional dorsal striatum were analyzed during the early and late learning phases of the 10-day sucrose self-administration process. We found that Arc protein is primarily detected in the DMS only in the initial learning stage; however, it is expressed in the DLS during both early and late learning stages. Moreover, Arc expression in the DMS correlated with the number of rewards received later in the training. These data indicated that the Arc expression in subregions of the dorsal striatum shows region-specific transfer and that Arc expression in the DMS contributes to obtaining reward in later learning stage during the process of instrumental learning.
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http://dx.doi.org/10.3389/fncel.2021.654521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170099PMC
May 2021

Endophytic fungi stimulate the concentration of medicinal secondary metabolites in thunb.

Plant Signal Behav 2021 Jun 6:1929731. Epub 2021 Jun 6.

School of Life Science, Guizhou Normal University, Guiyang Guizhou, China.

Endophytic fungi usually establish a symbiotic relationship with the host plant and affect its growth. In order to evaluate the impact of endophytic fungi on the Chinese herbal medicinal plant Thunb., three endophytes isolated from the rhizomes of , namely (IL), unidentified fungal sp. (UF), and (PC), were co-cultured individually with in sterile soil for 60 days. Analysis of the results showed that the endophytes stimulated the host plant in different ways: IL increased the growth of rhizomes and the accumulation of most of the phenolics and volatiles, UF promoted the accumulation of the medicinal compounds afzelin, decanal, 2-undecanone, and borneol without influencing host plant growth, and PC increased the fresh weight, total leaf area and height of the plants, as well as the growth of the rhizomes, but had only a small effect on the concentration of major secondary metabolites. Our results proved that the endophytic fungi had potential practical value in terms of the production of Chinese herbal medicines, having the ability to improve the yield and accumulation of medicinal metabolites.
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http://dx.doi.org/10.1080/15592324.2021.1929731DOI Listing
June 2021

The Preparation of a Novel Poly(Lactic Acid)-Based Sustained HS Releasing Microsphere for Rheumatoid Arthritis Alleviation.

Pharmaceutics 2021 May 18;13(5). Epub 2021 May 18.

State Key Laboratory of Quality Research in Chinese Medicine & School of Pharmacy, Macau University of Science and Technology, Macau SAR 999078, China.

Rheumatoid arthritis (RA) is a chronic, inflammatory autoimmune disease that mainly erodes joints and surrounding tissues, and if it is not treated in time, it can cause joint deformities and loss of function. S-propargyl-cysteine (SPRC) is an excellent endogenous hydrogen sulfide donor which can relieve the symptoms of RA through the promotion of HS release via the CSE/HS pathway in vivo. However, the instant release of HS in vivo could potentially limit its further clinical use. To solve this problem, in this study, a SPRC-loaded poly(lactic acid) (PLA) microsphere ([email protected]) was prepared, which could release SPRC in vitro in a sustained manner, and further promote sustained in vivo HS release. Furthermore, its therapeutical effect on RA in rats was also studied. A spherical-like [email protected] was successfully prepared with a diameter of approximately 31.61 μm, yielding rate of 50.66%, loading efficiency of 6.10% and encapsulation efficiency of 52.71%. The [email protected] showed significant prolonged in vitro SPRC release, to 4 days, and additionally, an in vivo HS release around 3 days could also be observed. In addition, a better therapeutical effect and prolonged administration interval toward RA rats was also observed in the [email protected] group.
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http://dx.doi.org/10.3390/pharmaceutics13050742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157395PMC
May 2021

A novel dendritic mesoporous silica based sustained hydrogen sulfide donor for the alleviation of adjuvant-induced inflammation in rats.

Drug Deliv 2021 Dec;28(1):1031-1042

State Key Laboratory of Quality Research in Chinese Medicine & School of Pharmacy, Macau University of Science and Technology, Taipa, China.

Purpose: S-propargyl-cysteine (SPRC), an excellent endogenous hydrogen sulfide (HS) donor, could elevate HS levels via the cystathionine γ-lyase CSE)/HS pathway both and . However, the immediate release of HS and daily administration of SPRC potentially limited its clinical use.

Methods: To solve the fore-mentioned problem, in this study, the dendritic mesoporous silica nanoparticles (DMSN) was firstly prepared, and a sustained HS delivery system consisted of SPRC and DMSN ([email protected]) was then constructed. Their release profiles, both and , were investigated, and their therapeutical effect toward adjuvant-induced arthritis (AIA) rats was also studied.

Results: The spherical morphology of DMSN could be observed under scanning Electron Microscope (SEM), and the transmission electron microscope (TEM) images showed a central-radiational pore channel structure of DMSN. DMSN showed excellent SPRC loading capacity and attaining a sustained releasing ability than SPRC both and , and the prolonged SPRC releasing could further promote the release of HS in a sustained manner through CSE/HS pathway both and . Importantly, the [email protected] showed promising anti-inflammation effect against AIA in rats was also observed.

Conclusions: A sustained HS releasing donor consisting of SPRC and DMSN was constructed in this study, and this sustained HS releasing donor might be of good use for the treatment of AIA.
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http://dx.doi.org/10.1080/10717544.2021.1921075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172227PMC
December 2021

Blood flow changes in the forearm arteries after ultrasound-guided costoclavicular brachial plexus blocks: a prospective observational study.

BMC Anesthesiol 2021 May 29;21(1):164. Epub 2021 May 29.

Department of Anesthesiology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, China.

Background: An increase in blood flow in the forearm arteries has been reported after brachial plexus block (BPB). However, few studies have quantitatively analysed the blood flow of the forearm arteries after BPB or have studied only partial haemodynamic parameters. The purpose of the present study was to comprehensively assess blood flow changes in the distal radial artery (RA) and ulnar artery (UA) after BPB performed via a new costoclavicular space (CCS) approach using colour Doppler ultrasound.

Methods: Thirty patients who underwent amputated finger replantation and received ultrasound-guided costoclavicular BPB were included in the study. The haemodynamic parameters of the RA and UA were recorded before the block and 10 min, 20 min, and 30 min after the block using colour Doppler ultrasound to determine the peak systolic velocity (PSV), end-diastolic velocity (EDV), mean velocity (V), pulsatility index (PI), resistance index (RI) and area. The volumetric flow rate (VFR) was calculated using the formula Q = area×V. The aforementioned parameters were compared not only before and after the BPB but also between the RA and UA.

Results: Compared with those of the respective baselines, there was a significant increase in the PSV, EDV, V, area, and VFR and a significant decrease in the PI and RI of the RA and UA 10 min, 20 min, and 30 min post-block. The increase 30 min post-block in EDV (258.68 % in the RA, 279.63 % in the UA) was the most notable, followed by that in the V (183.36 % in the RA, 235.24 % in the UA), and the PSV (139.11 % in the RA, 153.15 % in the UA) changed minimally. The V and VFR of the RA were significantly greater than those of the UA before the BPB; however, there was no significant difference in the VFR between the RA and UA after the BPB.

Conclusions: A costoclavicular BPB can increase blood flow in the forearm arteries. The RA had a higher volumetric flow rate than the UA before the BPB; however, the potential blood supply capacity of the UA was similar to that of the RA after a BPB.

Trial Registration: This study was registered at the Chinese Clinical Trial Registry (http://www.chictr.org.cn/searchproj.aspx, clinical trial number: ChiCTR 1900023796, date of registration: June 12, 2019).
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http://dx.doi.org/10.1186/s12871-021-01383-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164273PMC
May 2021

Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty.

Front Pharmacol 2021 7;12:649732. Epub 2021 May 7.

Department of Pharmacy, National Center for Children Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Fu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In this study, we analyze the components and mechanisms of FY in PP, based on the integrated pharmacology. Investigated main constituents, targets, pathways of FY by using an integrative pharmacology, and recognized main constituents by HPLC-MS/MS. Then, observed the levels of Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (E) in danazol-induced PP in Sprague-Dawley (SD) rats. Lastly, retrospective study analyzed the clinical data of 575 patients who were diagnosed PP, treated by the FY, and followed-up in our hospital from 2014-2020. The result that total of 116 important candidate targets were selected based on pharmacological analysis. Selected the top 10 values key targets such as the estrogen receptor alpha (ESR1) and insulin-like growth factor 1 (IGF1), were localized and the related gene functions were determined. Gene functions were associated with biological regulation, a cellular process, or signaling pathway, such as the Estrogen signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway. By recognizing the five compounds related to the ESR1 and IGF1, which are Quercetin, kaempferol, Luteolin, Apigenin, and Emodin. The results of the molecular docking study further showed that the flavonoids had a strong binding affinity for ESR1 and IGF1 after docking into the crystal structure. The results showed that the FY could effectively reduce E, LH, and FSH levels in SD rats. Furthermore, the results of the retrospective analysis of medical records showed that the FY could remarkably reduce E levels in girls with PP.
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http://dx.doi.org/10.3389/fphar.2021.649732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138182PMC
May 2021

Comparing Symptomatic and Asymptomatic Carotid Artery Atherosclerosis in Patients With Bilateral Carotid Vulnerable Plaques Using Magnetic Resonance Imaging.

Angiology 2021 May 21:33197211012531. Epub 2021 May 21.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, 118223Tsinghua University School of Medicine, Beijing, China.

We compared plaque characteristics between symptomatic and asymptomatic sides in patients with bilateral carotid vulnerable plaques using magnetic resonance imaging (MRI). Participants (n = 67; mean age: 65.8 ± 7.7 years, 61 males) with bilateral carotid vulnerable plaques were included. Vulnerable plaques were characterized by intraplaque hemorrhage (IPH), large lipid-rich necrotic core (LRNC), or fibrous cap rupture (FCR) on MRI. Symptomatic vulnerable plaques showed greater plaque burden, LRNC volume (median: 221.4 vs 134.8 mm, = .003), IPH volume (median: 32.2 vs 22.5 mm, = .030), maximum percentage (Max%) LRNC (median: 51.3% vs 41.8%, = .002), Max%IPH (median: 13.4% vs 9.5%, = .022), cumulative slices of LRNC (median: 10 vs 8, = .005), and more juxtaluminal IPH and/or thrombus (29.9% vs 6.0%, = .001) and FCR (37.3% vs 16.4%, = .007) than asymptomatic ones. After adjusting for plaque burden, differences in juxtaluminal IPH and/or thrombus (odds ratio [OR]: 5.49, 95% CI: 1.61-18.75, = .007) and FCR (OR: 2.90, 95% CI: 1.16-7.24, = .022) between bilateral sides remained statistically significant. For patients with bilateral carotid vulnerable plaques, symptomatic plaques had greater burden, more juxtaluminal IPH and/or thrombus, and FCR compared with asymptomatic ones. The differences in juxtaluminal IPH and/or thrombus and FCR between bilateral sides were independent of plaque burden.
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http://dx.doi.org/10.1177/00033197211012531DOI Listing
May 2021

Knockdown of CENPW Inhibits Hepatocellular Carcinoma Progression by Inactivating E2F Signaling.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211007253

Department of Infectious Diseases, Qingdao No.6 People's Hospital, Qingdao, Shandong, People's Republic of China.

Aim: This study aimed to evaluate the effects of centromere protein W (CENPW, also known as CUG2) in hepatocellular carcinoma (HCC).

Methods: CENPW expression in HCC tissues and cells was detected by RT-qPCR assay. CCK-8 and colony formation assay were used to assess cell proliferation. Wound healing and Transwell assay was used to detect cell migration and invasion, respectively. The flow cytometry was used to analyze the cell cycle distribution and apoptosis.

Results: CENPW expression was upregulated in HCC tissues and cells. Knockdown of CENPW inhibited cell proliferation, migration, and invasion and induced the G0/G1 phase arrest and cell apoptosis in HCC cells, which might involve the E2F signaling regulation.

Conclusion: CENPW acted as an oncogenic role in HCC progression via activation E2F signaling. Our findings may provide new insights into the studying mechanisms of HCC.
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http://dx.doi.org/10.1177/15330338211007253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120521PMC
May 2021

Genome-wide identification of superoxide dismutase gene families and their expression patterns under low-temperature, salt and osmotic stresses in watermelon and melon.

3 Biotech 2021 Apr 26;11(4):194. Epub 2021 Mar 26.

College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 Gansu China.

The growth and development of watermelon and melon are affected by abiotic stresses such as cold, salinity and drought. Plant superoxide dismutase (SOD) proteins exerted great effects on plant growth, development and response to abiotic stresses. However, little is known about the characteristics of watermelon and melon SOD gene families and their expression patterns under abiotic stresses. In this study, the genome-wide identification of genes and their expression patterns under abiotic stresses has been done in watermelon and melon. Seven were identified in watermelon and melon, respectively. Chromosome location indicated that the were dispersedly distributed on 4-6 chromosomes. Almost all the SOD proteins contained 300 amino acids or less and the intron numbers of ranged from 5 to 7. On the basis of phylogenetic analysis, the SODs were classified into six sub-groups which was also verified by similar motif composition, gene structure and sub-cellular location. Gene ontology analysis displayed that many SOD proteins participated in binding, catalytic, antioxidant activity and stimulus-response. -regulatory elements related to stresses and hormones were found in the promoters of the . Based on the quantitative real-time PCR, most of and genes showed obvious up-regulation under low-temperature, NaCl and PEG treatments. The abiotic stress-responsive genes were identified to improve watermelon and melon tolerance against abiotic stresses. This was a preliminary study to describe the genome-wide analysis of gene family in watermelon and melon, and the results would facilitate further study of gene cloning and functional verification of genes response to abiotic stresses in watermelon and melon.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-02726-7.
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http://dx.doi.org/10.1007/s13205-021-02726-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997938PMC
April 2021

Nitrogen-doped graphene supported Ni as an efficient and stable catalyst for levulinic acid hydrogenation.

Nanotechnology 2021 Apr 22. Epub 2021 Apr 22.

Guangdong University of Technology, Guangzhou, CHINA.

Transforming levulinic acid (LA) to γ-valerolactone (GVL) is a significant route for converting biomass into valuable chemicals. The development of an efficient and robust heterogeneous catalyst for this reaction has aroused great interest. In this work, nitrogen-doped graphene (NG) supported nickel (Ni) based heterogeneous catalyst with excellent activities was successfully synthesized. The Ni/NG catalyst shows outstanding performance for hydrogenation of LA to GVL at a relatively low temperature of 140 °C, which is superior then most of the present reported heterogeneous catalyst. Further investigation indicated the Ni nanoparticles were the active sites and the NG also plays an indispensable role. The catalytic performance was highly depended on the crystallinity, particles sizes and electronic structure of Ni in Ni/NG catalyst, which can be optimized by nitrogen doping. This work affords a new route for designing robust and excellent heterogeneous catalysts by doping method to optimize the support.
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http://dx.doi.org/10.1088/1361-6528/abfabbDOI Listing
April 2021

Recent Advances in Macrophage-Mediated Drug Delivery Systems.

Int J Nanomedicine 2021 7;16:2703-2714. Epub 2021 Apr 7.

Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, People's Republic of China.

Macrophages have been extensively used in the development of drug delivery systems, as they can prolong the circulation and release of drugs, extend their half-life, increase their stability and targeting ability, and reduce immunogenicity. Moreover, they have good biocompatibility and degradability and offer abundant surface receptors for targeted delivery of a wide variety of drugs. Macrophage-mediated drug delivery systems can be prepared by loading drugs or drug-loaded nanoparticles into macrophages, macrophage membranes or macrophage-derived vesicles. Although such systems can be used to treat inflammation, cancer, HIV infection and other diseases, they require further research and optimization since they have been assembled from diverse sources and therefore can have quite different physical and chemical properties. Moreover, potential cell-drug interactions can limit their application, and the biological activity of membrane proteins might be lost during membrane extraction and storage. In this review, we summarize the recent advances in this field and discuss the preparation of macrophage-mediated drug delivery systems, their advantages over other delivery systems, their potential applications and future lines of research.
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http://dx.doi.org/10.2147/IJN.S298159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039204PMC
May 2021

Virtual Surgical Planning and Three-Dimensional Printing to Aid the Anatomical Reduction of an Old Malunited Fracture of the Mandible.

J Craniofac Surg 2021 Mar 25. Epub 2021 Mar 25.

Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao School of Stomatology, Qingdao University, Qingdao Shandong, China.

Abstract: Mandible fracture is a common injury in maxillofacial surgery. It causes not only maxillofacial dysfunction but also facial deformities. Malunited fractures of the mandible have been a vast challenge in clinical treatment due to the misalignment of the broken ends and the occurrence of occlusal disorders. This case report describes using virtual surgical planning and three-dimensional printing to treat a patient with malunited fracture of the mandible. Failing to perform mandibular surgery due to severe brain trauma after the car accident, the patient got malunited healing of mandible. The authors applied virtual surgical planning to perform preoperative analysis and surgical design on this patient, three-dimensional printing to fabricate occlusal plate, and models of the preoperative and postoperative mandible to guide the operation. Finally, the authors achieved the reduction and reconstruction of the mandible with satisfactory clinical results.
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http://dx.doi.org/10.1097/SCS.0000000000007653DOI Listing
March 2021

Biomass activated carbon-derived imprinted polymer with multi-boronic acid sites for selective capture of glycoprotein.

J Colloid Interface Sci 2021 Aug 29;596:225-232. Epub 2021 Mar 29.

College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China. Electronic address:

Glycoproteins play crucial roles in many biological events such as protein folding, information transmission, nerve conduction, and molecular recognition. Some glycoproteins serve as disease biomarkers in clinical settings. However, selective detection of glycoprotein often faces great challenges, owing to its low abundance in complex biological samples. In this case, develop a highly sensitive and selective approach for glycoprotein detection is urgently needed. Molecularly imprinted polymers (MIPs) have proved to be an ideal absorbent material in detection and separation science. Herein, a novel biomass activated carbon-derived imprinted polymer ([email protected]/PBA/MIPs) was fabricated for selective recognition of glycoprotein. The as-prepared [email protected]/PBA/MIPs was synthesized using waste tea derived carbon as matrix, albumin chicken egg (OVA) as template, and dopamine as functional monomer. Branched polyethyleneimine (PEI) was covalently bonded on the BAC surface to increase the number of boronic acid moieties. Benefiting from the self-polymerization of dopamine and multi-boronic acid sites, a great number of recognition sites were presented under mild conditions. The static adsorption experiment showed that the [email protected]/PBA/MIPs exhibited a high binding capacity of 196.2 mg/g, rapid adsorption dynamics of 40 min, excellent selectivity and satisfactory reusability for OVA. Furthermore, the practicability of [email protected]/PBA/MIPs was verified by isolation of OVA from egg white. The good binding performance and facile preparation process make [email protected]/PBA/MIPs attractive for glycoprotein recognition, indicating its potential applications in biomedical research and clinical diagnostics.
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http://dx.doi.org/10.1016/j.jcis.2021.03.151DOI Listing
August 2021

Characterization and functional analysis of two acetylcholinesterase genes in Bradysia odoriphaga Yang et Zhang (Diptera: Sciaridae).

Pestic Biochem Physiol 2021 May 25;174:104807. Epub 2021 Feb 25.

Department of Entomology, China Agricultural University, Beijing 100193, China. Electronic address:

Two acetylcholinesterase genes (Boace1 and Boace2) were cloned from Bradysia odoriphaga, a devastating soil pest that mainly damages Chinese chives. The Boace1 encodes BoAChE1 protein consisting of 696 amino acid residues, while Boace2 encodes BoAChE2 containing 638 amino acids. Phylogenetic analysis showed that Boace1 and Boace2 are appeared to be distinct clusters. The gene expression patterns at different development stages and various body parts tissues were examined, and their biological functions were characterized by RNA interference and analog docking prediction. The results showed that both Boace genes were expressed in all developmental stages and examined tissues. The transcript level of Boace2 was significantly higher than Boace1 in all tested samples, and Boace1 was found most abundant in the head while Boace2 was highly expressed in the fat body of B. odoriphaga. The silencing of Boace1 and Boace2 significantly decreased the AChE activity of 36.6% and 14.8% separately, and increased the susceptibility of B. odoriphaga to phoxim, with 60.8% and 44.7% mortality. Besides, overexpression and gene duplication of Boace1 were found in two field resistant populations, and two major mutations, A319S and G400V, were detected in Boace1. Moreover, the docking results revealed that BoAChE1 had a higher affinity towards organophosphorus than BoAChE2. It is concluded that Boace2 is the most abundant ace type in B. odoriphaga, while both Boace play vital roles. Boace1 might play a major neurological function and more likely be the prime target for insecticides, while Boace2 might play some important unidentified roles.
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http://dx.doi.org/10.1016/j.pestbp.2021.104807DOI Listing
May 2021

The 3D-Printed Honeycomb Metamaterials Tubes with Tunable Negative Poisson's Ratio for High-Performance Static and Dynamic Mechanical Properties.

Materials (Basel) 2021 Mar 11;14(6). Epub 2021 Mar 11.

Tianjin Key Laboratory of Nonlinear Dynamics and Control, Tianjin University, Tianjin 300350, China.

The synthesized understanding of the mechanical properties of negative Poisson's ratio (NPR) convex-concave honeycomb tubes (CCHTs) under quasi-static and dynamic compression loads is of great significance for their multifunctional applications in mechanical, aerospace, aircraft, and biomedical fields. In this paper, the quasi-static and dynamic compression tests of three kinds of 3D-printed NPR convex-concave honeycomb tubes are carried out. The sinusoidal honeycomb wall with equal mass is used to replace the cell wall structure of the conventional square honeycomb tube (CSHT). The influence of geometric morphology on the elastic modulus, peak force, energy absorption, and damage mode of the tube was discussed. The experimental results show that the NPR, peak force, failure mode, and energy absorption of CCHTs can be adjusted by changing the geometric topology of the sinusoidal element. Through the reasonable design of NPR, compared with the equal mass CSHTs, CCHTs could have the comprehensive advantages of relatively high stiffness and strength, enhanced energy absorption, and damage resistance. The results of this paper are expected to be meaningful for the optimization design of tubular structures widely used in mechanical, aerospace, vehicle, biomedical engineering, etc.
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http://dx.doi.org/10.3390/ma14061353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000550PMC
March 2021

Short intracortical facilitation associates with motor-inhibitory control.

Behav Brain Res 2021 Jun 29;407:113266. Epub 2021 Mar 29.

Department of Rehabilitation Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China. Electronic address:

The ability of motor-inhibitory control is important in daily life. Inhibitory control deficits are commonly observed in psychiatric conditions with enhanced impulsivity. The physiological mechanisms underlying the inhibitory control deficits are not well elucidated. We systematically investigated the relationship between resting-state intracortical inhibition or facilitation and inhibitory control (indicated by stop signal reaction time, SSRT) to determine whether reduced intracortical inhibition or increased intracortical facilitation was related to the poorer inhibitory control. Thirty-three healthy subjects (age: 21.46 ± 1.40 years) participated in this study. We used paired-pulse transcranial magnetic stimulation to induce short intracortical inhibition, intracortical facilitation, long intracortical inhibition, and short intracortical facilitation at rest. SSRT was derived from stop signal task. We performed all measurements in two repeat sessions conducted two weeks apart. A negative correlation between short intracortical inhibition and SSRT was only observed in session 1; however, the correlation did not persist after controlling for short intracortical facilitation. Positive correlation between short intracortical facilitation and SSRT was observed in both sessions, indicating that individuals with greater resting-state short intracortical facilitation tend to have less efficient stopping performance. Our results help explain the inconsistency with respect to the relationship between short intracortical inhibition and SSRT in the existing literature. Short intracortical facilitation may be used as a potential physiological biomarker for motor-inhibitory control, which may have clinical implications for disorders associated with inhibitory control deficits.
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http://dx.doi.org/10.1016/j.bbr.2021.113266DOI Listing
June 2021

Influence of iTBS on the Acute Neuroplastic Change After BCI Training.

Front Cell Neurosci 2021 12;15:653487. Epub 2021 Mar 12.

Department of Rehabilitation Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.

Brain-computer interface (BCI) training is becoming increasingly popular in neurorehabilitation. However, around one third subjects have difficulties in controlling BCI devices effectively, which limits the application of BCI training. Furthermore, the effectiveness of BCI training is not satisfactory in stroke rehabilitation. Intermittent theta burst stimulation (iTBS) is a powerful neural modulatory approach with strong facilitatory effects. Here, we investigated whether iTBS would improve BCI accuracy and boost the neuroplastic changes induced by BCI training. Eight right-handed healthy subjects (four males, age: 20-24) participated in this two-session study (BCI-only session and iTBS+BCI session in random order). Neuroplastic changes were measured by functional near-infrared spectroscopy (fNIRS) and single-pulse transcranial magnetic stimulation (TMS). In BCI-only session, fNIRS was measured at baseline and immediately after BCI training. In iTBS+BCI session, BCI training was followed by iTBS delivered on the right primary motor cortex (M1). Single-pulse TMS was measured at baseline and immediately after iTBS. fNIRS was measured at baseline, immediately after iTBS, and immediately after BCI training. Paired-sample -tests were used to compare amplitudes of motor-evoked potentials, cortical silent period duration, oxygenated hemoglobin (HbO2) concentration and functional connectivity across time points, and BCI accuracy between sessions. No significant difference in BCI accuracy was detected between sessions ( > 0.05). In BCI-only session, functional connectivity matrices between motor cortex and prefrontal cortex were significantly increased after BCI training ('s < 0.05). In iTBS+BCI session, amplitudes of motor-evoked potentials were significantly increased after iTBS ('s < 0.05), but no change in HbO2 concentration or functional connectivity was observed throughout the whole session ('s > 0.05). To our knowledge, this is the first study that investigated how iTBS targeted on M1 influences BCI accuracy and the acute neuroplastic changes after BCI training. Our results revealed that iTBS targeted on M1 did not influence BCI accuracy or facilitate the neuroplastic changes after BCI training. Therefore, M1 might not be an effective stimulation target of iTBS for the purpose of improving BCI accuracy or facilitate its effectiveness; other brain regions (i.e., prefrontal cortex) are needed to be further investigated as potentially effective stimulation targets.
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http://dx.doi.org/10.3389/fncel.2021.653487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994768PMC
March 2021

Primary thymic atypical carcinoid with rare multiple bone metastasis: A case report and literature review.

Mol Clin Oncol 2021 Apr 25;14(4):78. Epub 2021 Feb 25.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.

Primary thymic atypical carcinoid (TAC) is a type of extremely rare neuroendocrine tumor. Approximately one-third of patients with TAC tumors are asymptomatic, and no treatments of TAC have been rigorously tested in multicenter clinical trials. To date, there has been no previous case report of TACs with multiple skull metastases. The present report describes a case of a 56-year-old male patient with a primary TAC and multiple axial skeleton metastases, including multiple bilateral metastases to the parietal bones, outer wall of the orbital cavities, and the petrous apex, as well as metastases to the suprasellar region and the clivus. The histological examination confirmed the diagnosis of TAC. Single-photon emission computed tomography, computed tomography and magnetic resonance imaging scans revealed multiple bone metastases. The patient refused chemotherapy and radiotherapy and only accepted alleviative treatment, and died 89 days after diagnosis. This type of cancer is highly malignant with an extremely short natural course and high disease-related mortality. In addition, a review of the literature was performed to provide a reference for proper diagnosis and treatment of TACs.
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http://dx.doi.org/10.3892/mco.2021.2240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947951PMC
April 2021

Prognostic efficacy of the combination of the pretreatment systemic Immune-Inflammation Index and Epstein-Barr virus DNA status in locally advanced Nasopharyngeal Carcinoma Patients.

J Cancer 2021 22;12(8):2275-2284. Epub 2021 Feb 22.

Cancer Center, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

The systemic immune-inflammation index (SII) and Epstein-Barr virus DNA (EBV DNA) levels has been used as a prognostic marker for nasopharyngeal carcinoma (NPC) patients, but there is no in-depth study in locally advanced NPC patients and no research on the predictive value of their combination. Our study aimed to evaluate the prognostic efficacy of the pretreatment SII, EBV DNA levels and their combination in locally advanced NPC patients receiving induction chemotherapy (IC) followed by concurrent chemoradiotherapy (CCRT). 319 patients diagnosed with locally advanced NPC receiving IC followed by CCRT were retrospectively reviewed (213 in the training cohort and 106 in the validation cohort). The cut-off value for the SII was determined using receiver operating characteristic (ROC) curve. Correlations between characteristics of patients were assessed using the Pearson correlation coefficient. Survival curves for the SII, EBV DNA levels and their combination were analyzed using the Kaplan-Meier method and compared using the log-rank test. Univariate and multivariate analyses were performed by the Cox proportional hazards regression model to evaluate the prognostic impact on overall survival (OS) and progression-free survival (PFS). A prognostic nomogram was generated and its prediction ability was measured by the concordance index (C-index). The optimal cutoff point for the SII was 402.10. A higher SII and EBV DNA positivity were demonstrated to be related to poorer survival outcomes ( < 0.05). Multivariate analyses showed that a higher SII, EBV DNA positivity and their combination were powerful independent risk factors for OS and PFS ( < 0.05). The SII - EBV DNA had the largest area under the curve (AUC) compared to either score alone. The incorporation of the SII - EBV DNA into established nomogram achieved higher C-index in the prediction of OS and PFS, indicating its superior for predicting survival. All results were found in the training cohort and confirmed in the validation cohort. The pretreatment SII and EBV DNA levels are promising factors for predicting survival in locally advanced NPC patients. The combination of them, which was superior to either score alone, was a complement to the conventional TNM staging system.
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http://dx.doi.org/10.7150/jca.52539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974890PMC
February 2021

Identification of key genes in hepatitis B associated hepatocellular carcinoma based on WGCNA.

Infect Agent Cancer 2021 Mar 16;16(1):18. Epub 2021 Mar 16.

School of Medicine, Nankai University, Tianjin, China.

Chronic Infection of Hepatitis B virus (HBV) is one risk factor of hepatocellular carcinoma (HCC). Much effort has been made to research the process of HBV-associated HCC, but its molecular mechanisms of carcinogenesis remain vague. Here, weighted gene co-expression network analysis (WGCNA) was employed to explore the co-expressed modules and hub/key genes correlated to HBV-associated HCC. We found that genes of the most significant module related to HBV-associated HCC were enriched in DNA replication, p53 signaling pathway, cell cycle, and HTLV-1 infection associated pathway; these cellular pathways played critical roles in the initiation and development of HCC or viral infections. Furthermore, seven hub/key genes were identified based on the topological network analysis, and their roles in HCC were verified by expression and Kaplan-Meier survival analysis. Protein-protein interaction and KEGG pathway analysis suggested that these key genes may stimulate cellular proliferation to promote the HCC progression. This study provides new perspectives to the knowledge of the key pathways and genes in the carcinogenesis process of HBV-associated HCC, and our findings provided potential therapeutic targets and clues of the carcinogenesis of HBV-associated HCC.
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http://dx.doi.org/10.1186/s13027-021-00357-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962393PMC
March 2021

REC8 promotes tumor migration, invasion and angiogenesis by targeting the PKA pathway in hepatocellular carcinoma.

Clin Exp Med 2021 Mar 7. Epub 2021 Mar 7.

Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, Hebei Clinical Research Center for Digestive Diseases, No. 215, Heping West Road, Shijiazhuang, 050000, Hebei, China.

REC8 is a member of the cohesin family, and its abnormal activation has been detected in cancer cells. This study explored the role and possible mechanism of REC8 in hepatocellular carcinoma (HCC). A total of 40 pairs of HCC and adjacent tissues were collected, and the clinical significance of REC8 expression in HCC was evaluated. REC8 expression in human HCC tissues and HCC cell lines was investigated by quantitative real-time PCR, Western blotting, immunohistochemistry and immunofluorescence staining. The biological functions of REC8 in HCC cell lines were detected by wound-healing assay, Matrigel invasion assay and tube formation assay. The proteins interacting with REC8 were identified by mass spectrometry after immunoprecipitation screening. There was a correlation between the high expression of REC8 and positive alpha-fetoprotein levels. The expression level of REC8 protein in HCC tissues was higher than that in adjacent tissues. REC8 has mainly located in the nucleus of HCC tissue cells and HCC cell lines, but it was expressed in the cytoplasm of adjacent normal tissue cells and hepatocytes. The results of wound healing, transwell invasion and tubular formation assays indicated that the overexpression of REC8 accelerated the metastasis of HCC in vitro; however, metastasis was suppressed after REC8 was silenced by small interference RNA. A total of 57 differentially expressed proteins were identified by mass spectrometry, and it was found that REC8 and PKA RII-α staining was colocalized in the nucleus. The expression levels of MMP-9 and VEGF-C were decreased after treatment with the PKA inhibitor H89. Overall, REC8 promotes the migration, invasion and angiogenesis of HCC cells through the PKA pathway.
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http://dx.doi.org/10.1007/s10238-021-00698-9DOI Listing
March 2021

Treatment of oral lichen planus by surgical excision and acellular dermal matrix grafting: Eleven case reports and review of literature.

World J Clin Cases 2021 Feb;9(6):1446-1454

Department of Stomatology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.

Background: Oral lichen planus (OLP) is a chronic inflammatory disorder, and it can affect normal oral function. The conventional treatments for OLP are not always effective, and relapse easily occurs. Therefore, treatment of OLP is difficult and challenging. In this study, we evaluated over a long period the clinical efficacy of surgical excision and acellular dermal matrix (ADM) grafting in patients with refractory OLP.

Case Summary: Eleven patients with refractory OLP underwent a standardized protocol of surgical excision and ADM grafting. The condition of the area of the grafted wound, the intraoperative maximum mouth opening, pain, and clinical healing were assessed at postoperative follow-up visits. All patients had a flat surgical area with similar mucosal tissue coverage and local scar formation. Patients had no irritation and pain in their mucous membranes when eating acidic and spicy food. All patients' mouth openings returned to normal within 2-6 mo after surgery. During follow-up, none of the patients had recurrence of OLP after surgery. The longest follow-up was 11 yr and the shortest was 6 mo, and none of the patients relapsed during follow-up.

Conclusion: Surgical excision and ADM grafting could be an effective method to treat refractory OLP.
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http://dx.doi.org/10.12998/wjcc.v9.i6.1446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896670PMC
February 2021

Iron-Based Metal-Organic Frameworks in Drug Delivery and Biomedicine.

ACS Appl Mater Interfaces 2021 Mar 19;13(8):9643-9655. Epub 2021 Feb 19.

Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.

Metal-organic frameworks (MOFs) are crystalline materials comprising metal centers and organic linkers that feature structural rigidity and functional flexibility. These attractive materials offer large surface areas, high porosity, and good chemical stability; they have shown promise in chemistry (H separation and catalysis), magnetism, and optics. They have also shown potential for drug delivery following the demonstration in 2006 that chromium-based MOFs can be loaded with ibuprofen. Since then, iron-based MOFs (Fe-MOFs) have been shown to offer high drug loading and excellent biocompatibility. The present review focuses on the synthesis and surface modifications of Fe-MOFs as well as their applications in drug delivery and biomedicine.
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http://dx.doi.org/10.1021/acsami.0c21486DOI Listing
March 2021

Occlusal Assessment of Zirconia Crowns Designed with the Digital Articulator and Traditional Methods.

Int J Prosthodont 2021 Jan-Feb;34(1):13-20

Purpose: To evaluate and compare the occlusal fit of zirconia crowns designed using a digital articulator method and the traditional method in a self-controlled clinical trial.

Materials And Methods: Two zirconia crowns each were prepared for 12 patients with a single posterior tooth defect using two different methods: a digital articulator method and the traditional method. In the traditional method, casts were scanned using a model scanner, and the relative positions of the maxillary and mandibular arches were determined by the intercuspal occlusion of the casts. In the digital articulator method, casts were mounted on a mechanical articulator and scanned, and the relative positions were determined by their respective positions in the articulator. Additional dynamic occlusal adjustments of the digital articulator crowns were performed. Both groups of zirconia crowns were milled in a five-axis milling machine. The time required for occlusal adjustments, the occlusal contacts, the occlusal contact distributions, the number of occlusal contacts, the relative occlusal forces, and patient satisfaction (visual analog scale score) were evaluated. Wilcoxon test, McNemar test, and paired t test were used to compare the parameters between the two methods.

Results: The occlusal adjustment times for the digital articulator and traditional method crowns were 327 ± 226 seconds and 395 ± 338 seconds, respectively (P > .05). There were no significant differences in occlusal contacts, occlusal contact distributions, number of occlusal contacts, relative occlusal forces, or VAS scores between the two methods (P > .05).

Conclusion: A digital articulator method for single-crown restoration was applied successfully. Crowns fabricated using a digital articulator or the traditional method can achieve acceptable occlusal fit for single-crown restorations.
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http://dx.doi.org/10.11607/ijp.6570DOI Listing
February 2021

[Construction of conjugated polymer-exoelectrogen hybrid bioelectrodes and applications in microbial fuel cells].

Sheng Wu Gong Cheng Xue Bao 2021 Jan;37(1):1-14

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Microbial fuel cell (MFC) is a bioelectrochemical device, that enables simultaneous wastewater treatment and energy generation. However, a few issues such as low output power, high ohmic internal resistance, and long start-up time greatly limit MFCs' applications. MFC anode is the carrier of microbial attachment, and plays a key role in the generation and transmission of electrons. High-quality bioelectrodes have developed into an effective way to improve MFC performance. Conjugated polymers have advantages of low cost, high conductivity, chemical stability and good biocompatibility. The use of conjugated polymers to modify bioelectrodes can achieve a large specific surface area and shorten the charge transfer path, thereby achieving efficient biological electrochemical performance. In addition, bacteria can be coated with nano-scale conjugated polymer and effectively transfer the electrons generated by cells to electrodes. This article reviews the recently reported applications of conjugated polymers in microbial fuel cells, focusing on the MFC anode materials modified by conjugated polymers. This review also systematically analyzes the advantages and limitations of conjugated polymers, and how these composite hybrid bioelectrodes solve practical issues such as low energy output, high inner resistance, and long starting time.
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http://dx.doi.org/10.13345/j.cjb.200253DOI Listing
January 2021

Translation and cross-cultural adaption of the Chinese version of the Vanderbilt Head and Neck Symptom Survey version 2.0: a tool for oral symptom assessment in head and neck cancer patients.

Health Qual Life Outcomes 2021 Jan 21;19(1):27. Epub 2021 Jan 21.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Patients with head and neck cancer (HNC) who are receiving radiotherapy commonly face detrimental complications, including oral issues. However, oral symptoms are not well understood given the lack of available specific assessment instruments. The Vanderbilt Head and Neck Symptom Survey version (VHNSS) 2.0 is an instrument specifically developed to identify oral symptoms in HNC patients receiving radiotherapy in the United States.

Objective: To perform the translation and cross-cultural adaptation of the original English version of VHNSS 2.0 into a Chinese version (Mainland China).

Methods: The translation and cultural adaptation process involved translation by independent translators, construction of a consensus version, back translation into the original English version, analysis by the expert committee and a pretest. The pretest was administered to 90 patients with HNC to assess the feasibility and practicality of the tool.

Results: The final Chinese version approved by the expert committee was well understood by all participants in the study. The instrument had satisfactory content validity, with indexes of 0.83 for semantic and idiomatic equivalence, 0.90 for cultural equivalence, and 0.91 for conceptual equivalence. Furthermore, this version had good internal consistency, with Cronbach's alpha coefficients ranging from 0.74 to 0.95.

Conclusion: The Chinese version of VHNSS 2.0 was translated and cross-culturally adapted for use in China. This translation is a feasible instrument to assess oral health-related quality of life in HNC patients undergoing radiotherapy and will be useful for symptom management by clinicians and researchers in China.
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http://dx.doi.org/10.1186/s12955-021-01673-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818719PMC
January 2021

Surgical Procedures in Veterans Affairs Hospitals During the COVID-19 Pandemic.

Ann Surg 2021 04;273(4):e129-e131

Surgical Service, Palo Alto Veterans Affairs Health Care System, Palo Alto, California.

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http://dx.doi.org/10.1097/SLA.0000000000004692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959859PMC
April 2021

The gender gap and healthcare: associations between gender roles and factors affecting healthcare access in Central Malawi, June-August 2017.

Arch Public Health 2020 Nov 17;78(1):119. Epub 2020 Nov 17.

Stanford University School of Medicine, 291 Campus Drive, Stanford, CA, 94305, USA.

Background: Women in low and middle-income countries (LMICs) do not have equal access to resources, such as education, employment, or healthcare compared to men. We sought to explore health disparities and associations between gender prioritization, sociocultural factors, and household decision-making in Central Malawi.

Methods: From June-August 2017, a cross-sectional study with 200 participants was conducted in Central Malawi. We evaluated respondents' access to care, prioritization within households, decision-making power, and gender equity which was measured using the Gender-Equitable Men (GEM) scale. Relationships between these outcomes and sociodemographic factors were analyzed using multivariable mixed-effect logistic regression.

Results: We found that women were less likely than men to secure community-sourced healthcare financial aid (68.6% vs. 88.8%, p < 0.001) and more likely to underutilize necessary healthcare (37.2% vs. 22.4%, p = 0.02). Both men and women revealed low GEM scores, indicating adherence to traditional gender norms, though women were significantly less equitable (W:16.77 vs. M:17.65, p = 0.03). Being a woman (Odds Ratio (OR) 0.41, 95% confidence interval (CI) 0.21-0.78) and prioritizing a woman as a decision-maker for large purchases (OR 0.38, CI 0.15-0.93) were independently associated with a lower likelihood of prioritizing women for medical treatment and being a member of the Chewa tribal group (OR 3.87, CI 1.83-8.18) and prioritizing women for education (OR 4.13, CI 2.13-8.01) was associated with a higher odds.

Conclusion: Women report greater barriers to healthcare and adhere to more traditional gender roles than men in this Central Malawian population. Women contribute to their own gender's barriers to care and economic empowerment alone is not enough to correct for these socially constructed roles. We found that education and matriarchal societies may protect against gender disparities. Overall, internal and external gender discrimination contribute to a woman's disproportionate lack of access to care.
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http://dx.doi.org/10.1186/s13690-020-00497-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672876PMC
November 2020