Publications by authors named "Qi Zhu"

502 Publications

Case Report: Prenatal Diagnosis and Treatment of Fetal Autoimmune-Associated First-Degree Atrioventricular Block: First Report From China.

Front Cardiovasc Med 2021 21;8:683486. Epub 2021 Jun 21.

Department of Paediatric Cardiology, West China Second University Hospital, Sichuan University, Chengdu, China.

The rapid progression from fetal first-degree atrioventricular block (AVB) to third-degree AVB had been reported. However, how to define fetal first-degree AVB with proper technique and the necessity of the treatment for fetal autoimmune-associated first-degree AVB are still controversial. To explore the diagnosis and the effect of treatment for fetal first-degree AVB. Four pregnant women with positive autoantibodies anti-SSA/Ro were admitted into our hospital with complaints of rapid prolonged atrioventricular (AV) intervals of their fetuses. Fetal AV intervals were re-measured by tissue Doppler imaging (TDI) from the onset of atrial contraction to ventricular systole (Aa-Sa), which were 170 ms (case 1-twin A), 160 ms (case 1-twin B), 163 ms (case 2) and 172 ms (case 3) and 170 ms (case 4), respectively. The histories of medication usage or infection during gestation were denied. Amniotic fluid genetic screenings and virological tests were negative in all cases. No structural cardiac disorders were found and the cardiovascular profile scores were 10 for each fetus. Oral dexamethasone (initial dose of 4.5 mg daily) and hydroxychloroquine (200 mg bid) plus weekly follow-up surveillance were suggested. The dosage of dexamethasone was adjusted according to the changes of the AV intervals and fetal development of biparietal diameters (BPD) and femur lengths (FL). All fetal AV intervals were controlled well. Maternal and fetal adverse effects were noted as diabetes in 1 mother and growth retardation in all fetuses. All fetuses were delivered cesarean section at 35, 37, 38, and 37 gestational weeks, with 10 scores of Apgar score. Postnatally, positive anti-SSA/Ro was found in all neonates. However, there were no clinical or laboratory evidence of neonatal lupus syndrome. No abnormal signs were found on postnatal electrocardiogram and echocardiography for all neonates. With a follow-up of 8-53 months, there was no progression of disease and all infants demonstrated normal physical, mental, and motor development. Prenatal treatment for fetal autoimmune-associated first-degree AVB could be an alternative. Strict surveillance and timely adjustment of the treatment according to the conditions of the mother and the fetus are indicated. Further studies are necessary to prove our concept.
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http://dx.doi.org/10.3389/fcvm.2021.683486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255611PMC
June 2021

Malate-aided selective crystallization and luminescence comparison of tetragonal and monoclinic LaVO:Eu nanocrystals.

Dalton Trans 2021 Jul;50(29):10147-10158

Research Center for Functional Materials, National Institute for Materials Science, Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan.

With malate (Mal2-) as a new type of chelate, tetragonal (t-) and monoclinic (m-) structured LaVO4:Eu crystals (∼10-60 nm) were selectively crystallized as nanosquares and nanorods via a hydrothermal reaction at 200 °C for 24 h. The effects of the Mal2-:(La,Eu)3+ molar ratio, solution pH and Eu3+ content on the phase structure and crystal morphology were systematically investigated and elucidated. The competition between OH- and Mal2- toward rare earth ions was discussed to play a critical role in phase selection, and the t-phase can only be fabricated at pH ∼ 6-8 with the assistance of Mal2-. The optimal Eu3+ content for luminescence was determined to be ∼5 at% under the VO43- → Eu3+ energy transfer mechanism. Experimental comparison showed that t-(La0.95Eu0.05)VO4 (λex = 275 nm, λem = 620 nm) emits ∼5.3 times as strong as m-(La0.95Eu0.05)VO4 does (λex = 313 nm, λem = 616 nm), while theoretical analysis revealed that the 5D0 level of Eu3+ has a quantum efficiency of ∼80% for the former and ∼70% for the latter. Besides, the t- and m-(La0.95Eu0.05)VO4 nanocrystal phosphors were analyzed to have fluorescence lifetimes of ∼1.53 ± 0.01 and 2.28 ± 0.01 ms for their 620 and 616 nm red emissions, respectively.
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http://dx.doi.org/10.1039/d1dt01644jDOI Listing
July 2021

HMGB2 orchestrates mitotic clonal expansion by binding to the promoter of C/EBPβ to facilitate adipogenesis.

Cell Death Dis 2021 Jul 2;12(7):666. Epub 2021 Jul 2.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China.

High-mobility group box 2 (HMGB2) is an abundant, chromatin-associated protein that plays an essential role in the regulation of transcription, cell proliferation, differentiation, and tumorigenesis. However, the underlying mechanism of HMGB2 in adipogenesis remains poorly known. Here, we provide evidence that HMGB2 deficiency in preadipocytes impedes adipogenesis, while overexpression of HMGB2 increases the potential for adipogenic differentiation. Besides, depletion of HMGB2 in vivo caused the decrease in body weight, white adipose tissue (WAT) mass, and adipocyte size. Consistently, the stromal vascular fraction (SVF) of adipose tissue derived from hmgb2 mice presented impaired adipogenesis. When hmgb2 mice were fed with high-fat diet (HFD), the body size, and WAT mass were increased, but at a lower rate. Mechanistically, HMGB2 mediates adipogenesis via enhancing expression of C/EBPβ by binding to its promoter at "GGGTCTCAC" specifically during mitotic clonal expansion (MCE) stage, and exogenous expression of C/EBPβ can rescue adipogenic abilities of preadipocytes in response to HMGB2 inhibition. In general, our findings provide a novel mechanism of HMGB2-C/EBPβ axis in adipogenesis and a potential therapeutic target for obesity.
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http://dx.doi.org/10.1038/s41419-021-03959-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253743PMC
July 2021

Natural Alkaloid Compounds as Inhibitors for Alpha-Synuclein Seeded Fibril Formation and Toxicity.

Molecules 2021 Jun 19;26(12). Epub 2021 Jun 19.

Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha 34110, Qatar.

The accumulation and aggregation of α-synuclein (α-syn) is the main pathologic event in Parkinson's disease (PD), dementia with Lewy bodies, and multiple system atrophy. α-Syn-seeded fibril formation and its induced toxicity occupy a major role in PD pathogenesis. Thus, assessing compounds that inhibit this seeding process is considered a key towards the therapeutics of synucleinopathies. Using biophysical and biochemical techniques and seeding-dependent cell viability assays, we screened a total of nine natural compounds of alkaloid origin extracted from Chinese medicinal herbs. Of these compounds, synephrine, trigonelline, cytisine, harmine, koumine, peimisine, and hupehenine exhibited in vitro inhibition of α-syn-seeded fibril formation. Furthermore, using cell viability assays, six of these compounds inhibited α-syn-seeding-dependent toxicity. These six potent inhibitors of amyloid fibril formation and toxicity caused by the seeding process represent a promising therapeutic strategy for the treatment of PD and other synucleinopathies.
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http://dx.doi.org/10.3390/molecules26123736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234408PMC
June 2021

Depression and anxiety symptoms to COVID-19 outbreak among the public, medical staff and patients during the initial phase of the pandemic: an online questionnaire survey by a WeChat Mini Program.

BMJ Open 2021 06 24;11(6):e046350. Epub 2021 Jun 24.

Department of Psychiatry, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

Objectives: To survey anxiety and depression symptoms to COVID-19 outbreak in the public, medical staff and patients during the initial phase of the pandemic.

Design: Cross-sectional online survey administered through WeChat Mini Program using Chinese versions of Zung Self-rating Depression Scale and Zung Self-rating Anxiety Scale.

Setting: Guangzhou, China.

Participants: 47 378 public, 1512 medical staff and 125 patients with COVID-19.

Results: Higher rates of depression (47.8%) and anxiety symptoms (48.7%) were shown by patients who were screened positive compared with those of the public (35.6%, 25.7%) or medical staff (15.4%, 13.3%). The professional identity of a nurse, conditions of 'with an infected family member' and 'working at the frontline' were risk factors to depression or anxiety symptoms for the medical staff. Younger age, lower educational level, female and not having adequate masks were the risk factors for the public.

Conclusion: The COVID-19 outbreak increased people's depression or anxiety emotion responses, which varied extensively among the patients, public and medical staff.
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http://dx.doi.org/10.1136/bmjopen-2020-046350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228573PMC
June 2021

MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI.

Stem Cell Res Ther 2021 06 16;12(1):348. Epub 2021 Jun 16.

Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Naval Medical University (Second Military Medical University), Shanghai, 200003, China.

Background: Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading to implant failure, implant revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, and we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection and transportation of miR-20a (sEV-20a). In this study, we intended to determine whether sEVs overexpressing miR-20a could exert a superior effect on osteoporotic bone defects and the underlying mechanism.

Methods: For evaluating the effect of sEV-20a on osteogenesis, in vitro and in vivo studies were performed. In vitro, we first showed that miR-20a was upregulated in the osteogenic process and overexpressed sEVs with miR-20a by the transfection method. Then, the proliferation, migration, and osteogenic differentiation abilities of hBM-MSCs treated with sEV-20a were detected by CCK-8 assays, alkaline phosphatase staining and alizarin red staining, qRT-PCR, and western blot. In vivo, we established an osteoporotic bone defect model and evaluated the effect of sEV-20a on bone formation by micro-CT, sequential fluorescent labeling, and histological analysis. To further explore the mechanism, we applied a bioinformatics method to identify the potential target of miR-20a.

Results: In vitro, sEV-20a was successfully established and proved to promote the migration and osteogenesis of hBM-MSCs. In vivo, sEV-20a promoted osteointegration in an osteoporotic rat model. To further elucidate the related mechanism, we proved that miR-20a could enhance osteogenesis by targeting BAMBI.

Conclusions: Collectively, the in vitro and in vivo results confirmed that MSC-derived sEV-20a therapy effectively promoted osteoporotic porous titanium alloy osteointegration via pro-osteogenic effects by targeting BAMBI.
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http://dx.doi.org/10.1186/s13287-021-02303-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207591PMC
June 2021

New Mg/Ge-Stabilized GdMgGeAlO:Ce Garnet Phosphor with Orange-Yellow Emission for Warm-White LEDs ( = 2.0-2.5).

Inorg Chem 2021 Jul 17;60(13):9773-9784. Epub 2021 Jun 17.

Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.

By stabilization of the GdAlO garnet by replacing 80% or more of Al with Mg/Ge pairs, a series of new orange-yellow-emitting GdMgGeAlO:Ce ( = 2.0-2.5) phosphors were successfully developed for potential application in warm-white-light-emitting diodes (WLEDs). Rietveld structure refinement proved that Mg first substitutes the octahedral Al ion, followed by the replacement of the tetrahedral Al together with Ge. The band structure of the = 2.0 typical garnet was analyzed via density functional theory (DFT) calculations. The incorporation of an increasing content of Mg/Ge was experimentally shown to narrow the band gap and expand the unit cell of the garnet host and blue shift the emission/excitation wavelength and shorten the fluorescence lifetime of Ce. The photoluminescence behaviors were rationalized by considering the influence of Mg/Ge on the crystal structure, band structure, and local coordination. An LED lamp fabricated by combining the (GdCe)MgGeAlO optimal phosphor with a 450 nm-emitting InGaN blue LED chip exhibited a color-rendering index of 71.6, luminous efficacy of 16.1 lm/W, and a low correlated color temperature of 2201 K under a driving current of 20 mA, indicating that phosphor may have potential application in warm WLEDs.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01072DOI Listing
July 2021

Plasmonic-doped melanin-mimic for CXCR4-targeted NIR-II photoacoustic computed tomography-guided photothermal ablation of orthotopic hepatocellular carcinoma.

Acta Biomater 2021 07 31;129:245-257. Epub 2021 May 31.

Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China; Institute of Digital Intelligence of Zhujiang Hospital of Southern Medical University, Guangzhou, 510280, China; Guangdong Provincial Clinical and Engineering Technology Center of Digital Medicine, Guangzhou, 510280, China. Electronic address:

Effective and noninvasive diagnosis and prompt treatment of early-stage hepatocellular carcinoma (HCC) are urgently needed to reduce its mortality rate. Herein, the integration of high-resolution diagnostic second near-infrared (NIR-II) photoacoustic computed tomography (PACT) and imaging-guided targeted photothermal ablation of orthotopic small HCC (SHCC) is presented for the first time, which was enabled by a plasmonic platinum (Pt)-doped polydopamine melanin-mimic nanoagent. As designed, an antibody-modified nanoagent (designated [email protected]) with a plasmonic blackbody-like NIR absorption and superior photothermal conversion efficiency (71.3%) selectively targeted and killed CXCR4-overexpressing HCC (HepG2) cells, which was validated in in vitro experiments. The targeted accumulation properties of [email protected] in vivo were previously recognized by demonstrating effective NIR-II PA imaging and photothermal ablation in a subcutaneous HCC mouse model. Subsequently, with real-time quantitative guidance by PACT for the accurate diagnosis of intraabdominal SHCC (approximately 4 mm depth), the effective and noninvasive photothermal ablation of SHCCs was successfully demonstrated in an orthotopic tumor-bearing mouse model without damaging adjacent liver tissues. These results show a great potential of NIR-II PACT-guided noninvasive photothermal therapy as an innovative phototheranostic approach and expand the biomedical applications of melanin-mimic materials. STATEMENT OF SIGNIFICANCE: In this paper, we report the first diagnostic NIR-II photoacoustic computed tomography (PACT)-guided noninvasive photothermal ablation of small hepatocellular carcinoma (SHCC) located in deep tissues in orthotopic tumor-bearing mice; this process is empowered by a polydopamine-based melanin-mimic tumor-targeting nanoagent doped with plasmonic platinum that provides superior NIR-II (1064 nm) absorption and photothermal conversion efficiency of 71.3%. Following surface modification with anti-CXCR4 antibodies, the nanoagent (namely [email protected]) can selectively target CXCR4-overexpressed HepG2 carcinoma cells and tumor lesions, and serve as the theranostic agent for both NIR-II PACT-based diagnosis of orthotopic SHCC (diameter less than 5 mm) and efficient NIR-II PTT in vivo. This study may also extend the potential of melanin-derived blackbody materials for optical-biomedical and water distillation applications.
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http://dx.doi.org/10.1016/j.actbio.2021.05.034DOI Listing
July 2021

Activation of human hepatic stellate cells enhances the metastatic ability of hepatocellular carcinoma cells via up-regulation of interleukin-1β.

J BUON 2021 Mar-Apr;26(2):435-443

Department of Gastroenterology, The Second Hospital of Fuzhou Affiliated Xiamen University, Fuzhou, Fujian, China.

Purpose: The purpose was to investigate the effect of activated human hepatic stellate cell (HSC) microenvironment on the metastatic capacity of hepatocellular carcinoma (HCC) cells and its underlying mechanism.

Methods: LX-2 HSCs were stimulated with Human Transforming Growth Factor-Beta 1(TGF-β1), and protein expression of α-smooth muscle actin (α-SMA) and filamentous actin (F-actin) were determined to verify the activation of LX-2 cells. Next, SMMC7721 HCC cells were cultured in the conditioned medium originating from activated LX-2 cells. Wound healing and Transwell assays were performed to examine cell migration and invasion. The expression of metastasis-related genes Matrix Metalloproteinase9 (MMP9), N-cadherin, and Vascular endothelial growth factor (VEGF) was detected. ELISA was carried out to determine the interleukin (IL) -1β level. Finally the inhibitors of TGF-β1 and IL-1β were employed to investigate the roles of LX-2 activation and IL-1β in the metastasis-related gene alterations.

Results: TGF-β1 activated LX-2 cells, as evidenced by up-regulated α-SMA and F-actin expression. Compared with the control medium, the conditioned medium derived from LX-2 cells significantly promoted the migration and invasion of SMMC7721 cells. And it also up-regulated mRNA and protein expression of the metastasis-related genes in SMMC7721 cells. Furthermore, it resulted in a significant increase in the IL-1β level in SMMC7721 cells. Importantly, TGF-β1 inhibitor and IL-1β inhibitor either individually or synergistically abolished the up-regulated expression of conditioned medium-induced metastasis-related gene in SMMC7721 cells.

Conclusions: The conditioned medium generating from TGF-β1-activated LX2 cells can enhance the metastatic ability of SMMC7721 cells through up-regulating IL-1 expression.
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June 2021

Common functional localizers to enhance NHP & cross-species neuroscience imaging research.

Neuroimage 2021 08 25;237:118203. Epub 2021 May 25.

Institut des Sciences Cognitives Marc Jeannerod, UMR 5229, Université de Lyon - CNRS, France. Electronic address:

Functional localizers are invaluable as they can help define regions of interest, provide cross-study comparisons, and most importantly, allow for the aggregation and meta-analyses of data across studies and laboratories. To achieve these goals within the non-human primate (NHP) imaging community, there is a pressing need for the use of standardized and validated localizers that can be readily implemented across different groups. The goal of this paper is to provide an overview of the value of localizer protocols to imaging research and we describe a number of commonly used or novel localizers within NHPs, and keys to implement them across studies. As has been shown with the aggregation of resting-state imaging data in the original PRIME-DE submissions, we believe that the field is ready to apply the same initiative for task-based functional localizers in NHP imaging. By coming together to collect large datasets across research group, implementing the same functional localizers, and sharing the localizers and data via PRIME-DE, it is now possible to fully test their robustness, selectivity and specificity. To do this, we reviewed a number of common localizers and we created a repository of well-established localizer that are easily accessible and implemented through the PRIME-RE platform.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118203DOI Listing
August 2021

Lycorine, a natural alkaloid, promotes the degradation of alpha-synuclein via PKA-mediated UPS activation in transgenic Parkinson's disease models.

Phytomedicine 2021 Jul 27;87:153578. Epub 2021 Apr 27.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China. Electronic address:

Background: Parkinson's disease (PD) is one of the most common neurodegenerative motor disorders, and is characterized by the presence of Lewy bodies containing misfolded α-synuclein (α-syn) and by selective degeneration of midbrain dopamine neurons. Studies have shown that upregulation of ubiquitin-proteasome system (UPS) activity promotes the clearance of aggregation-prone proteins such as α-syn and Tau, so as to alleviate the neuropathology of neurodegenerative diseases.

Purpose: To identify and investigate lycorine as a UPS enhancer able to decrease α-syn in transgenic PD models.

Methods: Dot blot was used to screen α-syn-lowering compounds in an inducible α-syn overexpression cell model. Inducible wild-type (WT) and mutant α-syn-overexpressing PC12 cells, WT α-syn-overexpressing N2a cells and primary cultured neurons from A53T transgenic mice were used to evaluate the effects of lycorine on α-syn degradation in vitro. Heterozygous A53T transgenic mice were used to evaluate the effects of lycorine on α-syn degradation in vivo. mCherry-GFP-LC3 reporter was used to detect autophagy-dependent degradation. Ub-R-GFP and Ub-G76V-GFP reporters were used to detect UPS-dependent degradation. Proteasome activity was detected by fluorogenic substrate Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC).

Results: Lycorine significantly promoted clearance of over-expressed WT and mutant α-syn in neuronal cell lines and primary cultured neurons. More importantly, 15 days' intraperitoneal administration of lycorine effectively promoted the degradation of α-syn in the brains of A53T transgenic mice. Mechanistically, lycorine accelerated α-syn degradation by activating cAMP-dependent protein kinase (PKA) to promote proteasome activity.

Conclusion: Lycorine is a novel α-syn-lowering compound that works through PKA-mediated UPS activation. This ability to lower α-syn implies that lycorine has the potential to be developed as a pharmaceutical for the treatment of neurodegenerative diseases, such as PD, associated with UPS impairment and protein aggregations.
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http://dx.doi.org/10.1016/j.phymed.2021.153578DOI Listing
July 2021

KDM4A regulates myogenesis by demethylating H3K9me3 of myogenic regulatory factors.

Cell Death Dis 2021 05 19;12(6):514. Epub 2021 May 19.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, North Third Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China.

Histone lysine demethylase 4A (KDM4A) plays a crucial role in regulating cell proliferation, cell differentiation, development and tumorigenesis. However, little is known about the function of KDM4A in muscle development and regeneration. Here, we found that the conditional ablation of KDM4A in skeletal muscle caused impairment of embryonic and postnatal muscle formation. The loss of KDM4A in satellite cells led to defective muscle regeneration and blocked the proliferation and differentiation of satellite cells. Myogenic differentiation and myotube formation in KDM4A-deficient myoblasts were inhibited. Chromatin immunoprecipitation assay revealed that KDM4A promoted myogenesis by removing the histone methylation mark H3K9me3 at MyoD, MyoG and Myf5 locus. Furthermore, inactivation of KDM4A in myoblasts suppressed myoblast differentiation and accelerated H3K9me3 level. Knockdown of KDM4A in vitro reduced myoblast proliferation through enhancing the expression of the cyclin-dependent kinase inhibitor P21 and decreasing the expression of cell cycle regulator Cyclin D1. Together, our findings identify KDM4A as an important regulator for skeletal muscle development and regeneration, orchestrating myogenic cell proliferation and differentiation.
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http://dx.doi.org/10.1038/s41419-021-03799-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134519PMC
May 2021

Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells.

Int J Nanomedicine 2021 10;16:3201-3216. Epub 2021 May 10.

Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People's Republic of China.

Purpose: Polyetheretherketone (PEEK) exhibits high mechanical strengths and outstanding biocompatibility but biological inertness that does not excite the cell responses and stimulate bone formation. The objective of this study was to construct submicro-nano structures on PEEK by femtosecond laser (FSL) for exciting the responses of MC3T3-E1 cells and gingival epithelial (GE) cells, which induce regeneration of bone/gingival tissues for long-term stability of dental implants.

Materials And Methods: In this study, submicro-nano structures were created on PEEK surface by FSL with power of 80 mW (80FPK) and 160 mW (160FPK).

Results: Compared with PEEK, both 80FPK and 160FPK with submicro-nano structures exhibited elevated surface performances (hydrophilicity, surface energy, roughness and protein absorption). Furthermore, in comparison with 80FPK, 160FPK further enhanced the surface performances. In addition, compared with PEEK, both 80FPK and 160FPK significantly excited not only the responses (adhesion, proliferation, alkaline phosphatase [ALP] activity and osteogenic gene expression) of MC3T3-E1 cells but also responses (adhesion as well as proliferation) of GE cells of human in vitro. Moreover, in comparison with 80FPK, 160FPK further enhanced the responses of MC3T3-E1 cells/GE cells.

Conclusion: FSL created submicro-nano structures on PEEK with elevated surface performances, which played crucial roles in exciting the responses of MC3T3-E1 cells/GE cells. Consequently, 160FPK with elevated surface performances and outstanding cytocompatibility would have enormous potential as an implant for dental replacement.
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http://dx.doi.org/10.2147/IJN.S303411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121686PMC
May 2021

Fumarylacetoacetate hydrolase is required for fertility in rice.

Planta 2021 May 18;253(6):122. Epub 2021 May 18.

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Main Conclusion: The rice OsFAH gene functions identically to that of Arabidopsis SSCD1 encoding FAH. Loss of OsFAH causes rice sterility. Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the tyrosine (Tyr) degradation pathway that is crucial for animals. By genetic analysis of the mutant of Short-day Sensitive Cell Death 1 gene encoding Arabidopsis FAH, we first found the pathway also plays a critical role in plants (Han et al., Plant Physiol 162:1956-1964, 2013). To further understand the role of the Tyr degradation pathway in plants, we investigated a biological function of the rice FAH. Firstly, the cDNA of rice FAH gene (OsFAH) was cloned and confirmed to be able to rescue the Arabidopsis Short-day Sensitive Cell Death 1 mutant defective in the FAH. Then, we identified the OsFAH T-DNA insertion mutant and generated the OsFAH RNA interference lines, and found that loss of OsFAH results in rice sterility. Furthermore, we analyzed expression of the OsFAH gene in roots, stems, leaves and young panicles at booting stage of rice and found that its transcript level was highest in young panicles and lowest in roots. In addition, the expression analysis of β-glucuronidase driven by OsFAH promoter in transgenic Arabidopsis showed that the OsFAH promoter was highly active in aerial tissues in vegetative stage, and sepals, filaments and stigma in reproductive stage. These results suggested that FAH plays an important role in rice fertility.
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http://dx.doi.org/10.1007/s00425-021-03632-1DOI Listing
May 2021

Reconstruction of the glutamate decarboxylase system in Lactococcus lactis for biosynthesis of food-grade γ-aminobutyric acid.

Appl Microbiol Biotechnol 2021 May 15;105(10):4127-4140. Epub 2021 May 15.

School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Gamma-aminobutyric acid (GABA), an important bioactive compound, is synthesized through the decarboxylation of L-glutamate (L-Glu) by glutamate decarboxylase (GAD). The use of lactic acid bacteria (LAB) as catalysts opens interesting avenues for the biosynthesis of food-grade GABA. However, a key obstacle involved in the improvement of GABA production is how to resolve the discrepancy of optimal pH between the intracellular GAD activity and cell growth. In this work, a potential GAD candidate (LpGadB) from Lactobacillus plantarum was heterologously expressed in Escherichia coli. Recombinant LpGadB existed as a homodimer under the native conditions with a molecular mass of 109.6 kDa and exhibited maximal activity at 40°C and pH 5.0. The K value and catalytic efficiency (k/K) of LpGadB for L-Glu was 21.33 mM and 1.19 mMs, respectively, with the specific activity of 26.67 μM/min/mg protein. Subsequently, four C-terminally truncated LpGadB mutants (GadB, GadB, GadB, GadB) were constructed based on homology modeling. Among them, the mutant GadB with highest catalytic activity at near-neutral pH values was selected. In further, the GadB and Glu/GABA antiporter (GadC) of Lactococcus lactis were co-overexpressed in the host L. lactis NZ3900. Finally, after 48 h of batch fermentation, the engineered strain L. lactis NZ3900/pNZ8149-gadBC yielded GABA concentration up to 33.52 g/L by applying a two-stage pH control strategy. Remarkably, this is the highest yield obtained to date for GABA from fermentation with L. lactis as a microbial cell factory.Key points• The GadB from L. plantarum was heterologously expressed in E. coli and biochemically characterized.• Deletion of the C-plug in GadB shifted its pH-dependent activity toward a higher pH.• Reconstructing the GAD system of L. lactis is an effective approach for improving its GABA production.
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http://dx.doi.org/10.1007/s00253-021-11328-5DOI Listing
May 2021

Grandparental care and sleep disturbances in preschool children: a population-based prospective cohort study.

Sleep Med 2021 06 10;82:165-171. Epub 2021 Apr 10.

Department of Developmental and Behavioral Pediatrics, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai, China; Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Objectives: Sleep disturbances are common in preschoolers, and profoundly affected by parenting. Grandparents are increasingly involved in childcare and have attracted worldwide attention for their potential impacts on child well-being. However, no studies have explored child sleep health within the context of grandparental care. We aimed to explore the association between grandparents as primary caregivers and children's sleep disturbances.

Methods: Participants included 20,324 preschoolers from the Shanghai Children's Health, Education and Lifestyle Evaluation-Preschool (SCHEDULE-P) in Shanghai, China. They were initially assessed in November 2016 and were reassessed in April 2018 and April 2019. Children's primary caregivers were collected at each survey. Sleep disturbances were assessed by the Children's Sleep Habits Questionnaire (CSHQ).

Results: At baseline, 84.4% of children had global sleep disturbances (CSHQ total score >41). Compared with parents, random-effects model indicated that grandparents as primary caregivers increased the adjusted odds ratio (OR) for children's global sleep disturbances by 30% (OR [95%CI]: 1.30 [1.21, 1.40]). To the specific domains of CSHQ, two-way repeated-measures ANOVA showed significant "caregiver transition" group × wave interaction in the behavioral sleep disturbances, such as "Bedtime Resistance" (p < 0.001). At each survey, children with grandparents as primary caregivers demonstrated significant higher behavioral sleep disturbances than their counterparts with parental care.

Conclusions: Grandparental care is associated with increased sleep disturbances, particularly behavioral sleep disturbances, in preschoolers. Future studies should explore the underlying mechanisms and whether sleep programs targeting grandparents can decrease children's sleep disturbances.
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http://dx.doi.org/10.1016/j.sleep.2021.03.033DOI Listing
June 2021

Favipiravir in the treatment of patients with SARS-CoV-2 RNA recurrent positive after discharge: A multicenter, open-label, randomized trial.

Int Immunopharmacol 2021 Aug 21;97:107702. Epub 2021 Apr 21.

Department of Infectious Disease, Center for Liver Disease, Peking University First Hospital, Beijing, China; Department of Infectious Disease, Peking University International Hospital, Beijing, China. Electronic address:

Background: The clinical characteristics and treatment of patients who tested positive for COVID-19 after recovery remained elusive. Effective antiviral therapy is important for tackling these patients. We assessed the efficacy and safety of favipiravir for treating these patients.

Methods: This is a multicenter, open-label, randomized controlled trial in SARS-CoV-2 RNA re-positive patients. Patients were randomly assigned in a 2:1 ratio to receive either favipiravir, in addition to standard care, or standard care alone. The primary outcome was time to achieve a consecutive twice (at intervals of more than 24 h) negative RT-PCR result for SARS-CoV-2 RNA in nasopharyngeal swab and sputum sample.

Results: Between March 27 and May 9, 2020, 55 patients underwent randomization; 36 were assigned to the favipiravir group and 19 were assigned to the control group. Favipiravir group had a significantly shorter time from start of study treatment to negative nasopharyngeal swab and sputum than control group (median 17 vs. 26 days); hazard ratio 2.1 (95% CI [1.1-4.0], p = 0.038). The proportion of virus shedding in favipiravir group was higher than control group (80.6% [29/36] vs. 52.6% [10/19], p = 0.030, respectively). C-reactive protein decreased significantly after treatment in the favipiravir group (p = 0.016). The adverse events were generally mild and self-limiting.

Conclusion: Favipiravir was safe and superior to control in shortening the duration of viral shedding in SARS-CoV-2 RNA recurrent positive after discharge. However, a larger scale and randomized, double-blind, placebo-controlled trial is required to confirm our conclusion.
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http://dx.doi.org/10.1016/j.intimp.2021.107702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059985PMC
August 2021

High-Performance Flexible Self-Powered Photodetectors Utilizing Spontaneous Electron and Hole Separation in Quasi-2D Halide Perovskites.

Small 2021 Jun 23;17(23):e2100442. Epub 2021 Apr 23.

Department of Materials Science and Engineering, City University of Hong Kong, Kowloon, 999077, Hong Kong SAR.

Although there are recent advances in many areas of quasi-2D halide perovskites, photodetectors based on these materials still cannot achieve satisfactory performance for practical applications where high responsivity, fast response, self-powered nature, and excellent mechanical flexibility are urgently desired. Herein, utilizing one-step spin-coating method, self-assemble quasi-2D perovskite films with graded phase distribution in the order of increasing number of metal halide octahedral layers are successfully prepared. Gradient type-II band alignments along the out-of-plane direction of perovskites with spontaneous separation of photo-generated electrons and holes are obtained and then employed to construct self-powered vertical-structure photodetectors for the first time. Without any driving voltage, the device exhibits impressive performance with the responsivity up to 444 mA W and ultrashort response time down to 52 µs. With a bias voltage of 1.5 V, the device responsivity becomes 3463 mA W with the response speed as fast as 24 µs. Importantly, the device's mechanical flexibility is greatly enhanced since the photocurrent prefers flowing through the metal halide octahedral layers between the top and bottom contact electrodes in the vertical device structure, being more tolerant to film damage. These results evidently indicate the potential of graded quasi-2D perovskite phases for next-generation optoelectronic devices.
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http://dx.doi.org/10.1002/smll.202100442DOI Listing
June 2021

Minimal specifications for non-human primate MRI: Challenges in standardizing and harmonizing data collection.

Neuroimage 2021 08 18;236:118082. Epub 2021 Apr 18.

Laboratory for Neuro- and Psychophysiology, Department of Neurosciences, KU Leuven Medical School, Leuven 3000, Belgium; Leuven Brain Institute, KU Leuven, Leuven 3000, Belgium; Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA 02129, USA; Department of Radiology, Harvard Medical School, Boston, MA 02144, USA.

Recent methodological advances in MRI have enabled substantial growth in neuroimaging studies of non-human primates (NHPs), while open data-sharing through the PRIME-DE initiative has increased the availability of NHP MRI data and the need for robust multi-subject multi-center analyses. Streamlined acquisition and analysis protocols would accelerate and improve these efforts. However, consensus on minimal standards for data acquisition protocols and analysis pipelines for NHP imaging remains to be established, particularly for multi-center studies. Here, we draw parallels between NHP and human neuroimaging and provide minimal guidelines for harmonizing and standardizing data acquisition. We advocate robust translation of widely used open-access toolkits that are well established for analyzing human data. We also encourage the use of validated, automated pre-processing tools for analyzing NHP data sets. These guidelines aim to refine methodological and analytical strategies for small and large-scale NHP neuroimaging data. This will improve reproducibility of results, and accelerate the convergence between NHP and human neuroimaging strategies which will ultimately benefit fundamental and translational brain science.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118082DOI Listing
August 2021

Case Report: Transarterial Chemoembolization in Combination With Tislelizumab Downstages Unresectable Hepatocellular Carcinoma Followed by Radical Salvage Resection.

Front Oncol 2021 29;11:667555. Epub 2021 Mar 29.

Department of General Surgery, Shanghai Organ Transplantation Medical Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Introduction: Transarterial chemoembolization (TACE) is inefficient at converting unresectable hepatocellular carcinoma (uHCC) to resectable. Treatment with immune checkpoint inhibitors (ICIs) is an emerging strategy for uHCC. Combined therapy of TACE with ICIs is considered to improve the therapeutic effect.

Case Presentation: A 45-year-old man was diagnosed with a bulky HCC under cirrhotic background without distant metastasis. Curative resection was infeasible, and TACE plus tislelizumab (an ICI targeting PD-1) was applied. The treatment course, starting from TACE and followed by tislelizumab one week later, was repeated every four weeks. After three courses, the tumor showed striking shrink in volume with complete radiological response, which permitted salvage resection. Notably, pathological examination found complete necrosis of the tumor with massive infiltration of lymphocytes in the tumor-nontumor interface and extensive granulomatous inflammation in the surrounding nontumor liver, indicating activated immune response synergistically caused by TACE with tislelizumab. The patient is now living well without tumor recurrence for 6 months after surgery.

Conclusion: TACE in combination with tislelizumab may represent a potent strategy for uHCC. Data from randomized clinical trials are needed to assess its safety and effect in the setting of preoperative downstaging therapy.
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http://dx.doi.org/10.3389/fonc.2021.667555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044844PMC
March 2021

Associations between sleep duration patterns and cognitive decline trajectories in older Chinese adults.

Aging Clin Exp Res 2021 Apr 10. Epub 2021 Apr 10.

Department of Preventive Medicine, North Sichuan Medical College, Fujiang Road, Shunqing, Nanchong, 234, Sichuan, China.

Background: Abnormal sleep duration has been identified as a risk factor for cognitive decline. However, most studies have measured sleep duration and cognitive function at a single time point, while both tend to decrease with age.

Aims: This study aimed to investigate the association between changes in sleep duration patterns and cognitive trajectories in older adults.

Methods: Using longitudinal data (2005-2014) from the Chinese Longitudinal Healthy Longevity Survey, a total of 4347 subjects were included in this study. Cognitive function was measured with the Chinese version of the Mini-Mental State Examination. Group-based trajectory models were used to identify the trajectories of sleep duration and cognitive decline within the study sample as the participants aged. Furthermore, the association between sleep duration patterns and cognitive trajectories was examined by multinomial logistic regression analysis.

Results: Using the medium-stable sleep duration pattern as a reference, the trajectory with a long and increased sleep duration pattern was more likely to be associated with the slight cognitive decline trajectory (OR = 1.80; 95% CI 1.31-2.44). Persistent short sleep was associated with a decreased likelihood of having a rapid cognitive decline trajectory (OR = 0.32; 95% CI 0.12-0.75).

Discussion: Changes in sleep duration over time were independently associated with cognitive decline and may be a marker of cognitive decline.

Conclusions: Tracking of sleep duration over time in older adults may provide a useful insight for prevention of cognitive decline, and the increased sleep duration should be noticed with greater vigilance.
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http://dx.doi.org/10.1007/s40520-021-01851-wDOI Listing
April 2021

The relationship between SARS-COV-2 RNA positive duration and the risk of recurrent positive.

Infect Dis Poverty 2021 Mar 31;10(1):45. Epub 2021 Mar 31.

Department of Infectious Disease, Center for Liver Disease, Peking University First Hospital, Beijing, China.

Background: The management of discharge COVID-19 patients with recurrent positive SARS-CoV-2 RNA is challenging. However, there are fewer scientific dissertations about the risk of recurrent positive. The aim of this study was to explore the relationship between SARS-COV-2 RNA positive duration (SPD) and the risk of recurrent positive.

Methods: This case-control multi-center study enrolled participants from 8 Chinese hospital including 411 participants (recurrent positive 241). Using unadjusted and multivariate-adjusted logistic regression analyses, generalized additive model with a smooth curve fitting, we evaluated the associations between SPD and risk of recurrent positive. Besides, subgroup analyses were performed to explore the potential interactions.

Results: Among recurrent positive patients, there were 121 females (50.2%), median age was 50 years old [interquartile range (IQR): 38-63]. In non-adjusted model and adjusted model, SPD was associated with an increased risk of recurrent positive (fully-adjusted model: OR = 1.05, 95% CI: 1.02-1.08, P = 0.001); the curve fitting was not significant (P = 0.286). Comparing with SPD < 14 days, the risk of recurrent positive in SPD > 28 days was risen substantially (OR = 3.09, 95% CI: 1.44-6.63, P = 0.004). Interaction and stratified analyses showed greater effect estimates of SPD and risk of recurrent positive in the hypertension, low monocyte count and percentage patients (P for interaction = 0.008, 0.002, 0.036, respectively).

Conclusion: SPD was associated with a higher risk of recurrent positive and especially SPD > 28 day had a two-fold increase in the relative risk of re-positive as compared with SPD < 14 day. What's more, the risk may be higher among those with hypertension and lower monocyte count or percentage.
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http://dx.doi.org/10.1186/s40249-021-00831-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010778PMC
March 2021

Single-cell analysis reveals innate immunity dynamics in ankylosing spondylitis.

Clin Transl Med 2021 03;11(3):e369

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, and Human Phenome Institute, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1002/ctm2.369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982614PMC
March 2021

Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model.

Sci Rep 2021 Mar 25;11(1):6906. Epub 2021 Mar 25.

Department of Human Sciences, The Ohio State University, 302D Wiseman Hall, 400 W 12th Ave, Columbus, OH, 43210, USA.

Emerging evidence has highlighted the connection between exposure to air pollution and the increased risk of obesity, metabolic syndrome, and comorbidities. Given the recent interest in studying the effects of ultrafine particle (UFP) on the health of obese individuals, this study examined the effects of gastrointestinal UFP exposure on gut microbial composition and metabolic function using an in vivo murine model of obesity in both sexes. UFPs generated from light-duty diesel engine combustion of petrodiesel (B0) and a petrodiesel/biodiesel fuel blend (80:20 v/v, B20) were administered orally. Multi-omics approaches, including liquid chromatography-mass spectrometry (LC-MS) based targeted metabolomics and 16S rRNA gene sequence analysis, semi-quantitatively compared the effects of 10-day UFP exposures on obese C57B6 mouse gut microbial population, changes in diversity and community function compared to a phosphate buffer solution (PBS) control group. Our results show that sex-specific differences in the gut microbial population in response to UFP exposure can be observed, as UFPs appear to have a differential impact on several bacterial families in males and females. Meanwhile, the alteration of seventy-five metabolites from the gut microbial metabolome varied significantly (ANOVA p < 0.05) across the PBS control, B0, and B20 groups. Multivariate analyses revealed that the fuel-type specific disruption to the microbial metabolome was observed in both sexes, with stronger disruptive effects found in females in comparison to male obese mice. Metabolic signatures of bacterial cellular oxidative stress, such as the decreased concentration of nucleotides and lipids and increased concentrations of carbohydrate, energy, and vitamin metabolites were detected. Furthermore, blood metabolites from the obese mice were differentially affected by the fuel types used to generate the UFPs (B0 vs. B20).
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http://dx.doi.org/10.1038/s41598-021-85784-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994449PMC
March 2021

Laboratory observations for two-dimensional solute transport in an aquifer-aquitard system.

Environ Sci Pollut Res Int 2021 Aug 18;28(29):38664-38678. Epub 2021 Mar 18.

School of Environmental Studies, China University of Geosciences, NO. 68 Jincheng Street, East Lake High-Tech, 17 Development Zone, Wuhan, Hubei, 430074, People's Republic of China.

Low-permeability media such as clay appear in nearly all hydrogeological systems. To date, although significant efforts have been put forward by hydrologists, transport mechanism is still not well understood in such media, especially in an aquifer-aquitard system. In this study, two-dimensional experiments of groundwater flow and solute transport were conducted in a clay-sand two-layer system to investigate the characteristics of flow and transport in such a system. Sodium chloride (NaCl) (a conservative tracer) from a tank was injected after passing by the pre-inlet reservoir where the mixing effect and flow transiency were analyzed. A new numerical model considering the mixing effect and flow transiency was developed to interpret the experimental data based on the finite-element COMSOL Multiphysics platform. Transport parameters were assessed by best fitting the observed breakthrough curves (BTCs). Several important results were obtained. Firstly, aquitard advection was found to be non-negligible and should be considered in a proper mathematical model for describing the transport process. Secondly, advective velocities were temporally variable and showed decreasing trends in the sand and clay layers, mainly due to the impacts of physical and biological clogging. Thirdly, the mixing effect in the pre-inlet reservoir led to a lower tracer concentration in the sand layer at early times. Finally, the observed BTCs exhibited early arrivals in the clay layer, possibly resulting from preferential flow pathways. These findings can provide hints for contamination remediation works in aquifer-aquitard systems.
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http://dx.doi.org/10.1007/s11356-021-13123-1DOI Listing
August 2021

"Stay-at-Home" Lifestyle Effect on Weight Gain during the COVID-19 Outbreak Confinement in China.

Int J Environ Res Public Health 2021 02 12;18(4). Epub 2021 Feb 12.

School of Public Health, Nantong University, Nantong 226019, China.

In February 2020, a novel coronavirus (SARS-COV2) broke out in Wuhan city of China. The Chinese government decisively imposed nationwide confinement. This study comprised a structured, online questionnaire, based on 40 items inquiring about socio-demographic information and anthropometric data (reporting weight and height), as well as changes in food intake, physical activity, and sleep during the COVID-19 outbreak. Questionnaires were distributed to residents of Jiangsu and other provinces from 29 March to 5 April. A total of 889 respondents were included, aged between 16 and 70 years (61% females). There was a significant increase in total food intake by 9.8% and a slight increase by 29.2% of respondents, and a significant decrease in physical activity by 31.5% and a slight decrease by 23.4% of respondents, especially in snacks and drinks, and outdoor activities. The rate of weight gain in the total population was 30.6% and the average weight gain was 0.5 ± 2.8 kg. The main factors contributing to weight gain were increased food intake and reduced physical activity. Additionally, normal-weight people were more likely to gain weight than people with overweight/obesity during the COVID-19 confinement. This study provided a good warning and educational reference value on lifestyle changes during the COVID-19 confinement.
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http://dx.doi.org/10.3390/ijerph18041813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918476PMC
February 2021

Combined effects of weight change trajectories and eating behaviors on childhood adiposity status: A birth cohort study.

Appetite 2021 07 23;162:105174. Epub 2021 Feb 23.

Department of Developmental and Behavioral Pediatrics, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; Pediatric Translational Medicine Institution, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; MOE-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China. Electronic address:

Previous studies have suggested that infant rapid weight change can be associated with an increased weight later in life. However, the weight change trajectory in early life over time and which childhood lifestyle behaviors may modify the risk of rapid weight change have not been characterized. Using our ongoing birth cohort study, we have addressed these issues. Nine follow-up time points (birth, 3, 6, 9, 12, 18, 24, 36, and 48 months) were used to calculate the change between two adjacent weight-for-age z-scores (WAZ-change), and then WAZ-change trajectories were defined via group-based trajectory modeling. The solitary, independent and combined effects of WAZ-change trajectories and each lifestyle factor (eating behaviors, physical activity, media exposure time and total sleep duration) on childhood adiposity measures at age 4 years were determined using multivariate regression analysis. Overall, 84 (38%) children had a steady growth trajectory from birth to 4 years, while the other 137 (62%) children had an early infancy rapid growth trajectory, particularly in the first three months. Compared to children with steady growth, children with early infancy rapid growth had a significantly higher body mass index, waist circumference, and subcutaneous fat. Moreover, weight change trajectory and three eating behaviors (i.e. food responsiveness, satiety responsiveness and food fussiness), not only had independent effects, but also combined (synergistic) effects on the majority of adiposity measures. Our results extend the current literature and provide a potentially valuable model to aid clinicians and health professionals in designing early-life interventions targeting specific populations, specific ages and specific lifestyle behaviors to prevent childhood overweight/obesity.
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http://dx.doi.org/10.1016/j.appet.2021.105174DOI Listing
July 2021

On the role of Zr substitution in structure modification and photoluminescence of LiLa(TaZr)O:Eu garnet phosphors.

Dalton Trans 2021 Mar;50(9):3337-3347

Research Center for Functional Materials, National Institute for Materials Science, Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan.

Solid-state reaction at 1000 °C produces a series of Li-stuffed Li5+2x(La1-yEuy)3(Ta1-xZrx)2O12 garnet phosphors (x = 0-1, y = 0.05-0.6) that exhibit favorable efficiency and thermal stability for red luminescence under either blue or n-UV light excitation, where the optimal composition was identified to be x = 0.5 and y = 0.4. The concentration quenching of luminescence was determined to occur via electric dipole-dipole interactions. Zr4+ substitution for Ta5+, accompanied by additional Li+ for charge compensation, was found via Rietveld structure refinement and Raman/UV-Vis spectroscopy to profoundly affect the tetrahedral and octahedral occupancies of Li, the symmetry of (La/Eu)O8 dodecahedron, and the bandgap of the host lattice and cation disorder, with which the systematically varying excitation and emission behaviors of Eu3+ were deciphered. The Li6(La0.6Eu0.4)3(Ta0.5Zr0.5)2O12 optimal phosphor showed quantum yields of ∼40 and 48% under 393 and 463 nm excitations, respectively, a fluorescence lifetime of ∼0.66 ms for its main emission at 610 nm, color coordinates of around (0.653, 0.347), and can retain as high as ∼85% of its room-temperature emission intensity at 423 K. The phosphor also exhibited a favorable performance for n-UV excited LED lighting.
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http://dx.doi.org/10.1039/d0dt04245eDOI Listing
March 2021

Cohort Profile: The Shanghai Children's Health, Education and Lifestyle Evaluation, Preschool (SCHEDULE-P) study.

Int J Epidemiol 2021 05;50(2):391-399

Department of Developmental and Behavioral Pediatrics, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1093/ije/dyaa279DOI Listing
May 2021

Comprehensive Perspectives on Experimental Models for Parkinson's Disease.

Aging Dis 2021 Feb 1;12(1):223-246. Epub 2021 Feb 1.

1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

Parkinson's disease (PD) ranks second among the most common neurodegenerative diseases, characterized by progressive and selective loss of dopaminergic neurons. Various cross-species preclinical models, including cellular models and animal models, have been established through the decades to study the etiology and mechanism of the disease from cell lines to nonhuman primates. These models are aimed at developing effective therapeutic strategies for the disease. None of the current models can replicate all major pathological and clinical phenotypes of PD. Selection of the model for PD largely relies on our interest of study. In this review, we systemically summarized experimental PD models, including cellular and animal models used in preclinical studies, to understand the pathogenesis of PD. This review is intended to provide current knowledge about the application of these different PD models, with focus on their strengths and limitations with respect to their contributions to the assessment of the molecular pathobiology of PD and identification of the therapeutic strategies for the disease.
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http://dx.doi.org/10.14336/AD.2020.0331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801282PMC
February 2021
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