Publications by authors named "Qi Zheng"

562 Publications

Downregulation of triggering receptor expressed on myeloid cells 1 inhibits invasion and migration of liver cancer cells by mediating macrophage polarization.

Oncol Rep 2021 Apr 2;45(4):1-12. Epub 2021 Mar 2.

Department of Hepatology, Hepatology Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350005, P.R. China.

Triggering receptor expressed on myeloid cells‑1 (TREM1) is a cell‑surface protein expressed on tumor‑associated macrophages (TAMs), the predominant inflammatory cells in the tumor microenvironment; however, the mechanisms for the influence of TREM1 on TAM polarization during liver cancer progression have not been investigated. In the present study, 20 patients diagnosed with hepatocellular carcinoma (HCC) who underwent surgery were enrolled, and TREM1 expression on M1/M2 macrophages and on M2 macrophages was assessed by immunohistochemical staining. Human leukemia monocytic cells (THP‑1) were differentiated into M2 macrophages using phorbol 12‑myristate 13‑acetate, IL‑4 and IL‑13. A specific short hairpin RNA was used to knockdown TREM1 expression. To investigate the effects of TREM1 downregulation in macrophages on the migration and invasion of liver cancer cells, HepG2 and MHCC97H cell lines were co‑cultured with specific conditioned media. Reverse transcription‑quantitative PCR and western blot analyses were used to detect M1 and M2 macrophage marker expression. The expression levels of proteins of the PI3K/AKT/mTOR signaling pathway were analyzed by western blotting, revealing that TREM1 expression in HCC tissues was significantly elevated compared with that in adjacent normal tissues, and TREM1 was highly expressed on the cell membranes of M2 macrophages in tumor tissues compared with in adjacent normal tissues. The present results demonstrated that TREM1 downregulation in macrophages shifted M2 macrophages towards an M1 phenotype, as defined by higher expression levels of M1‑associated markers and decreased expression levels of M2‑associated markers. In addition, TREM1 downregulation in macrophages suppressed migration and invasion of HepG2 and MHCC97H cells. Furthermore, TREM1‑knockdown in macrophages inhibited PI3K/AKT/mTOR activation in the polarization of M2 macrophages. In conclusion, downregulation of TREM1 expression in macrophages shifted M2 macrophages towards a M1 phenotype via inhibiting PI3K/AKT signaling. In addition, migration and invasion of HepG2 and MHCC97H cells were inhibited when this signaling pathway was blocked. The present findings suggest TREM1 as a novel potential therapeutic target for liver cancer management.
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http://dx.doi.org/10.3892/or.2021.7988DOI Listing
April 2021

miR-130a-3p regulates steroid hormone synthesis in goat ovarian granulosa cells by targeting the PMEPA1 gene.

Theriogenology 2021 Feb 19;165:92-98. Epub 2021 Feb 19.

College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, 230036, China; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Anhui Hefei, 230036, China. Electronic address:

MicroRNAs (miRNAs) are key epigenomic regulators of proliferation, differentiation, and secretion in cells involved in follicular development. We here studied the functional role of one such molecule, miR-130a-3p, in goat ovarian granulosa cells (GCs). High expression of this miRNA was evident in goat GCs by fluorescence in situ hybridization and suppressed estradiol and progesterone secretion from these cells, as determined by ELISA. miR-130a-3p was predicted to have a binding site for the 3' UTR of the prostate transmembrane protein androgen induced 1 gene (PMEPA1), and this was verified by a dual-luciferase reporter assay. PMEPA1 mRNA and protein expression were both found to be regulated by miR-130a-3p in GCs. Moreover, the overexpression or knockdown of PMEPA1 enhanced or suppressed estradiol and progesterone secretion from these cells, respectively. Furthermore, the secretion of estradiol and progesterone did not change significantly after the offsetting of PMEPA1 overexpression in GCs by miR-130a-3p. In summary, our present data indicate that miR-130a-3p inhibits the secretion of estradiol and progesterone in GCs by targeting PMEPA1. Our study thus provides seminal data and important new insights into the regulation of reproductive mechanisms in the nanny goat and other female mammals.
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http://dx.doi.org/10.1016/j.theriogenology.2021.02.012DOI Listing
February 2021

Corrigendum: Dual-Targeting Nanoparticle-Mediated Gene Therapy Strategy for Hepatocellular Carcinoma by Delivering Small Interfering RNA.

Front Bioeng Biotechnol 2021 11;9:656268. Epub 2021 Feb 11.

Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.3389/fbioe.2020.00512.].
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http://dx.doi.org/10.3389/fbioe.2021.656268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906225PMC
February 2021

Si-Ni-San ameliorates chronic colitis by modulating type I interferons-mediated inflammation.

Phytomedicine 2021 Feb 10;84:153495. Epub 2021 Feb 10.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address:

Background: Ulcerative colitis (UC) is a chronic relapsing inflammatory disease that markedly elevates the risk of colon cancers and results in disability. The disrupted immune homeostasis has been recognized as a predominant player in the pathogenesis of UC. However, the overall remission rate of current therapies based on immunoregulation is still unsatisfactory. Si-Ni-San (SNS) has been found effective in relieving UC through thousands of years of clinical practice, yet the specific mechanisms of the protective effect of SNS were not fully elucidated.

Purpose: We aim to investigate the therapeutic effects of SNS against the development of chronic colitis and the underlying mechanisms.

Methods: We established a DSS-induced chronic experimental colitis mouse model to evaluate the effect of SNS. RNA-sequencing, bioinformatic analysis, and in vitro studies were performed to investigate the underlying mechanisms.

Results: Our data demonstrated that SNS significantly ameliorated chronic experimental colitis via inhibiting the expression of genes associated with inflammatory responses. Interestingly, SNS significantly suppressed DSS-induced type I interferon (IFN) responses instead of directly downregulating the production of pro-inflammatory cytokines, such as Il-6. In vitro study further found that SNS selectively inhibited STING and RIG-I pathway-induced type I IFN responses by modulating TBK1- and IRF3-dependent signaling transduction. SNS also suppressed the expression of IFN-stimulated genes by directly inhibiting STAT1 and STAT2 activation.

Conclusion: Our study not only provides novel insights into the pathogenic role of type I IFN responses in colitis but also suggested that SNS or bioactive compounds derived from SNS may serve as novel therapeutic strategies for the treatment of UC via interfering type I IFN-mediated inflammation.
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http://dx.doi.org/10.1016/j.phymed.2021.153495DOI Listing
February 2021

Sudden Hypotension and Increased Serum IFN-γ and IL-10 Predict Early Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis Patients.

J Pediatr 2021 Feb 10. Epub 2021 Feb 10.

Department of Rheumatology Immunology & Allergy, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, Hangzhou, 310052, China. Electronic address:

Objective: To identify clinical and laboratory predictors for early macrophage activation syndrome (MAS) associated with systemic juvenile idiopathic arthritis (sJIA).

Study Design: This is a retrospective cohort study of 149 sJIA patients, of which 27 patients had 31 episodes of MAS. We evaluated the clinical and laboratory features of sJIA patients with MAS and compared them with those without MAS. We focused our analysis on the overall process of MAS development, especially MAS onset.

Results: As shown in previous studies, we found a high percentage of fever, absence of arthritis, and central nervous system dysfunction at MAS onset in our study cohort. We also found that 35% MAS patients had hypotension although not shock, and 22.6% MAS patients had gastrointestinal involvement at MAS onset. Compared with MAS patients without hypotension, MAS patients with hypotension had higher ICU admission, presented with more arthritis, serositis, pneumonia, and gastrointestinal involvement, and had higher white blood cell and absolute neutrophil counts and serum bilirubin levels, and lower serum total protein. We confirmed laboratory markers such as platelet counts, lactate dehydrogenase, and aspartate aminotransferase can help to identify early MAS and that ferritin: ESR ratio of around 20.0 had a high diagnostic sensitivity and specificity for MAS. In addition, we discovered that the combination of interferon (IFN)-γ >17.1 pg/mL and interleukin (IL)-10 >7.8 pg/mL appeared to be a good cytokine pattern for the recognition of MAS onset.

Conclusion: Sudden hypotension, elevated ferritin: ESR ratio, and the cytokine pattern of significantly increased IFN-γ and IL-10 levels are important markers for early identification of MAS in addition to the traditional characteristics of sJIA-associated MAS.
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http://dx.doi.org/10.1016/j.jpeds.2021.02.008DOI Listing
February 2021

Application of 3-dimensional printing technology combined with guide plates for thoracic spinal tuberculosis.

Medicine (Baltimore) 2021 Feb;100(6):e24636

Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicinel/Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China.

Background: To explore the accuracy and security of 3-dimensional (3D) printing technology combined with guide plates in the preoperative planning of thoracic tuberculosis and the auxiliary placement of pedicle screws during the operation.

Methods: Retrospective analysis was performed on the data of 60 cases of thoracic tuberculosis patients treated with 1-stage posterior debridement, bone graft fusion, and pedicle screw internal fixation in the Department of Orthopedics, Zhejiang Chinese Medicine and Western Medicine Integrated Hospital from March 2017 to February 2019. There were 31 males and 29 females; age: 41 to 52 years old, with an average of (46.6 ± 2.0) years old. According to whether 3D printing personalized external guide plates are used or not, they are divided into 2 groups: 30 cases in 3D printing group (observation group), and 30 cases in pedicle screw placement group (control group). A 1:1 solid model of thoracic spinal tuberculosis and personalized pedicle guide plates was created using the 3D printing technology combined with guide plates in the observation group. Stability and accuracy tests were carried out in vitro and in vivo. 30 patients in the control group used conventional nail placement with bare hands. The amount of blood loss, the number of fluoroscopy, the operation time, and the occurrence of adverse reactions related to nail placement were recorded. After the operation, the patients were scanned by computed tomography to observe the screw position and grade the screw position to evaluate the accuracy of the navigation template. All patients were followed up for more than 1 year. Visual Analogue Scale scores, erythrocyte sedimentation rate, and C-reactive protein were evaluated before surgery, 6 months after surgery, and 12 months after surgery.

Results: Sixty patients were followed up for 6 to 12 months after surgery. One hundred seventy-five and 177 screws were placed in the 3D printing group and the free-hand placement group, respectively. The rate of screw penetration was only 1.14% in the 3D-printed group (all 3 screws were grade 1) and 6.78% in the free-hand nail placement group (12 screws, 9 screws were grade 1 and 3 screws were grade 2). The difference was statistically significant (P = .047). The operation time of the 3D printing group ([137.67 ± 9.39] minutes), the cumulative number of intraoperative fluoroscopy ([4.67 ± 1.03] times), and the amount of intraoperative blood loss ([599.33 ± 83.37] mL) were significantly less than those in the manual nail placement group ([170.00 ± 20.48] minutes, [9.38 ± 1.76] times, [674.6 ± 83.61] mL). The differences were statistically significant (P < .05). There was no significant difference in VAS score and Oswestry disability index score between the 2 groups of patients before operation, 3 and 6 months after operation (P > .05).

Conclusion: The 3D printing technology combined with guide plate is used in thoracic spinal tuberculosis surgery to effectively reduce the amount of bleeding, shorten the operation time, and increase the safety and accuracy of nail placement.
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http://dx.doi.org/10.1097/MD.0000000000024636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886418PMC
February 2021

Acute hepatotoxicity and nephrotoxicity risk assessment of the Tibetan medicine 25 flavors of the turquoise pill based on H-NMR metabonomics.

J Ethnopharmacol 2021 Feb 8:113916. Epub 2021 Feb 8.

Beijing Hospital of Tibetan Medicine, China Tibetology Research Center, Beijing 100029, China. Electronic address:

Ethnopharmacological Relevance: 25 flavors of the turquoise pill, a traditional Tibetan medicine for the treatment of various types of hepatitis, has not been investigated on its safety, especially the component mineral turquoise, which is believed to be essential but worried for its potential toxicity.

Aim Of The Study: To explore the potential acute toxicity and function of 25 flavors of the turquoise pill and turquoise, the possible mechanism of the effects of turquoise and 25 flavors of the turquoise pill were systematically studied based on H NMR metabolomics.

Materials And Methods: The rats were administered with turquoise and 25 flavors of the turquoise pill by gavage for 7 days, and samples of serum, liver, and kidney were collected. The potential toxicity and function of turquoise and 25 flavors of the turquoise pill on the liver and kidney of SD rats were evaluated by H NMR metabonomics, histopathology, and biochemical indexes.

Results: The results demonstrated that 25 flavors of the turquoise pill could scavenge free oxygen radicals, strengthen aerobic respiration and inhibit glycolysis in the liver. It did not cause oxidative stress in the kidney with no obvious damage. By modulation of branched-chain amino acids (BCAAs), 25 flavors of the turquoise pill can improve the utilization of glucose and promote aerobic respiration of the kidney.

Conclusion: Considering the high dosage and short duration used in this study relative to their typical clinical usage, administration of 25 flavors of the turquoise pill and its component mineral turquoise are safe to livers and kidneys.
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http://dx.doi.org/10.1016/j.jep.2021.113916DOI Listing
February 2021

The COMPASS-like complex modulates fungal development and pathogenesis by regulating H3K4me3-mediated targeted gene expression in Magnaporthe oryzae.

Mol Plant Pathol 2021 Feb 8. Epub 2021 Feb 8.

Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, China.

Histone-3-lysine-4 (H3K4) methylation is catalysed by the multiprotein complex known as the Set1/COMPASS or MLL/COMPASS-like complex, an element that is highly evolutionarily conserved from yeast to humans. However, the components and mechanisms by which the COMPASS-like complex targets the H3K4 methylation of plant-pathogenic genes in fungi remain elusive. Here we present a comprehensive analysis combining biochemical, molecular, and genome-wide approaches to characterize the roles of the COMPASS-like family in the rice blast fungus Magnaporthe oryzae, a model plant pathogen. We purified and identified six conserved subunits of COMPASS from M. oryzae: MoBre2 (Cps60/ASH2L), MoSpp1 (Cps40/Cfp1), MoSwd2 (Cps35), MoSdc1 (Cps25/DPY30), MoSet1 (MLL/ALL), and MoRbBP5 (Cps50), using an affinity tag on MoBre2. We determined the sequence repeat in dual-specificity kinase splA and ryanodine receptors domain of MoBre2 can interact directly with the DPY30 domain of MoSdc1 in vitro. Furthermore, we found that deletion of the genes encoding COMPASS subunits of MoBre2, MoSPP1, and MoSwd2 caused similar defects regarding invasive hyphal development and pathogenicity. Genome-wide profiling of H3K4me3 revealed that it has remarkable co-occupancy at the transcription start site regions of target genes. Significantly, these target genes are often involved in spore germination and pathogenesis. Decreased gene expression caused by the deletion of MoBre2, MoSwd2, or MoSpp1 was highly correlated with a decrease in H3K4me3. These results suggest that MoBre2, MoSpp1, and MoSwd2 function as a whole COMPASS complex, contributing to fungal development and pathogenesis by regulating H3K4me3-targeted genes in M. oryzae.
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http://dx.doi.org/10.1111/mpp.13035DOI Listing
February 2021

Investigation of transcriptome profile of ischemia/reperfusion injury of abdominal skin flaps in rats after methane-rich saline treatment using RNA-seq.

Clin Hemorheol Microcirc 2021 Feb 3. Epub 2021 Feb 3.

Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, China.

Objective: Here we examined the influence of methane-rich saline treatment (MS) on the whole transcriptome of the skin flaps during the ischemia/reperfusion (I/R) injuryusing RNA-sequence (RNA-seq).

Methods: The rats were divided into three groups: the sham surgery group (SH),the I/R surgery group treated with physiological saline (I/R-P) or the I/R surgery group treated with the methane-rich saline (I/R-M) respectively. On the 72 hours after operation, the perfusion and the distribution of micro-circulatoryblood flow in skin flaps were observed by laser doppler flowmeters. The whole transcriptome alteration of the skin flaps was examined using RNA-seq. Moreover, the responses of the skin flaps to MRS treatment were examined using bio-informatic and q-PCR approaches after I/R injury.

Results: The methane-rich saline (MS) treatment could expand survival area and improve the blood perfusion of the skin flaps after l/R injury. Compared to the I/R-P group, 474 genes significantly altered in the I/R-M group. These genes were mainly associated the development, the cell adhesion and migration. In addition, the PI3K-Akt signal pathway was meaningfully related to regulation of MS treatment. Q-PCR results confirmed that MS treatment positively regulated PI3K-Akt signal pathway relative genes and inhibited the cell adhesion relative genes.

Conclusion: These results proved that methane-rich saline may alleviate I/R injury and improve flap survival rate by regulating cell adhesion and PI3K-Akt signal pathway.
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http://dx.doi.org/10.3233/CH-201046DOI Listing
February 2021

Association Between Childhood Maltreatment and Symptoms of Obsessive-Compulsive Disorder: A Meta-Analysis.

Front Psychiatry 2020 20;11:612586. Epub 2021 Jan 20.

Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China.

Previous studies have indicated that childhood maltreatment (CM) may potentially influence the clinical symptomatology of obsessive-compulsive disorder (OCD). Here, we aimed to quantify the relationship between CM and obsessive-compulsive symptoms (OCS) and depressive symptoms in OCD through a meta-analysis. We searched PubMed, Embase, Cochrane Library, and PsycARTICLES databases for articles reporting the association between CM and OCD on April 15, 2020. Random-effect models were used to quantify the relationship between CM and the severity of OCS and depressive symptoms in OCD. Ten records with 1,611 OCD patients were included in the meta-analysis. The results revealed that CM is positively correlated with the severity of OCS [ = 0.10, 95%Confidence Interval (CI): 0.01-0.19, = 0.04] as well as depressive symptoms in OCD ( = 0.15, 95%CI: 0.07-0.24, = 0.0002). For the subtypes of CM, childhood emotional abuse (CEA) and childhood sexual abuse (CSA) was related with the severity of OCS ( = 0.11, 95%CI: 0.03-0.19, = 0.009) and obsession ( = 0.13, 95%CI: 0.03-0.23, = 0.01), respectively. Our meta-analysis indicates that OCD patients who suffered more CM may exhibit more severe OCS and depressive symptoms.
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http://dx.doi.org/10.3389/fpsyt.2020.612586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854900PMC
January 2021

Case of bullous pemphigoid induced by apatinib mesylate.

Br J Clin Pharmacol 2021 Feb 3. Epub 2021 Feb 3.

Department of Integrated TCM & Western Medicine, Shanghai Dermatology Hospital, Tongji University School of Medicine, Shanghai, 200443, China.

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http://dx.doi.org/10.1111/bcp.14583DOI Listing
February 2021

Counterfactual Thinking-Related Emotional Responses in Patients With Major Depressive Disorder.

Front Psychiatry 2020 8;11:589335. Epub 2021 Jan 8.

Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China.

To explore the emotional characteristics of counterfactual thinking (CT)-related emotion responses in patients with major depressive disorder (MDD) via the "counterfactual thinking gambling task (CTGT)." Twenty-five patients with MDD (the MDD group) and twenty-five healthy controls (the HC group) with matched demographic features were included. The 17-item Hamilton Depression Scale (HAMD) and the 14-item Hamilton Anxiety Rating Scale (HAMA) were used to assess the severity of depression and anxiety symptoms. The counterfactual thinking gambling task was applied to assess the situation-focused- and behavior-focused-CT-related emotion responses in the MDD group and the HC group. There was no significant difference in general demographic data between the two groups ( > 0.05). Compared with the HC group, the MDD group experienced higher levels of "" and lower levels of "" in the situation-focused CT paradigm ( < 0.05). However, the experience of "" and "" in the behavior-focused CT paradigm were not significantly different between the two groups ( > 0.05). MDD is associated with an impaired situation-focused-CT-related emotion responses, and is often accompanied by increased disappointment and decreased joy; however, behavior-focused-CT-related emotion responses are not significantly affected in MDD. This pattern may represent the characteristic CT-related emotion responses of MDD.
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http://dx.doi.org/10.3389/fpsyt.2020.589335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820069PMC
January 2021

Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.

Transgenic Res 2021 Feb 22;30(1):91-104. Epub 2021 Jan 22.

Applied StemCell, Inc., Milpitas, CA, USA.

The goal of this study is to establish a Cre/loxP rat resource for conditional and physiologically predictive rat models of human diseases. The laboratory rat (R. norvegicus) is a central experimental animal in several fields of biomedical research, such as cardiovascular diseases, aging, infectious diseases, autoimmunity, cancer models, transplantation biology, inflammation, cancer risk assessment, industrial toxicology, pharmacology, behavioral and addiction studies, and neurobiology. Up till recently, the ability of creating genetically modified rats has been limited compared to that in the mouse mainly due to lack of genetic manipulation tools and technologies in the rat. Recent advances in nucleases, such as CRISPR/Cas9 (clustered regularly-interspaced short palindromic repeats/CRISPR associated protein 9), as well as TARGATT™ integrase system enables fast, efficient and site-specific introduction of exogenous genetic elements into the rat genome. Here, we report the generation of a collection of tissue-specific, inducible transgenic Cre rats as tool models using TARGATT™, CRISPR/Cas9 and random transgenic approach. More specifically, we generated Cre driver rat models that allow controlled gene expression or knockout (conditional models) both temporally and spatially through the Cre-ERT2/loxP system. A total of 10 Cre rat lines and one Cre reporter/test line were generated, including eight (8) Cre lines for neural specific and two (2) lines for cardiovascular specific Cre expression. All of these lines have been deposited with the Rat Resource and Research Center and provide a much-needed resource for the bio-medical community who employ rat models for their studies of human diseases.
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http://dx.doi.org/10.1007/s11248-020-00226-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854434PMC
February 2021

Genome-wide association study of yield and related traits in common wheat under salt-stress conditions.

BMC Plant Biol 2021 Jan 7;21(1):27. Epub 2021 Jan 7.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Soil salinization is a major threat to wheat production. It is essential to understand the genetic basis of salt tolerance for breeding and selecting new salt-tolerant cultivars that have the potential to increase wheat yield.

Result: In this study, a panel of 191 wheat accessions was subjected to genome wide association study (GWAS) to identify SNP markers linked with adult-stage characters. The population was genotyped by Wheat660K SNP array and eight phenotype traits were investigated under low and high salinity environments for three consecutive years. A total of 389 SNPs representing 11 QTLs were significantly associated with plant height, spike number, spike length, grain number, thousand kernels weight, yield and biological mass under different salt treatments, with the phenotypic explanation rate (R) ranging from 9.14 to 50.45%. Of these, repetitive and pleiotropic loci on chromosomes 4A, 5A, 5B and 7A were significantly linked to yield and yield related traits such as thousand kernels weight, spike number, spike length, grain number and so on under low salinity conditions. Spike length-related loci were mainly located on chromosomes 1B, 3B, 5B and 7A under different salt treatments. Two loci on chromosome 4D and 5A were related with plant height in low and high salinity environment, respectively. Three salt-tolerant related loci were confirmed to be important in two bi-parental populations. Distribution of favorable haplotypes indicated that superior haplotypes of pleiotropic loci on group-5 chromosomes were strongly selected and had potential for increasing wheat salt tolerance. A total of 14 KASP markers were developed for nine loci associating with yield and related traits to improve the selection efficiency of wheat salt-tolerance breeding.

Conclusion: Utilizing a Wheat660K SNPs chip, QTLs for yield and its related traits were detected under salt treatment in a natural wheat population. Important salt-tolerant related loci were validated in RIL and DH populations. This study provided reliable molecular markers that could be crucial for marker-assisted selection in wheat salt tolerance breeding programs.
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http://dx.doi.org/10.1186/s12870-020-02799-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792188PMC
January 2021

Enrichment and characterization of an effective hexavalent chromium-reducing microbial community YEM001.

Environ Sci Pollut Res Int 2021 Jan 6. Epub 2021 Jan 6.

Hubei Engineering Research Center for Biological Jiaosu, China Three Gorges University, Yichang, 443002, China.

Chromium (Cr) is one of the most widely used heavy metals in industrial processes, resulting in water and soil pollution that seriously threaten environmental safety. In this paper, we have directionally enriched a Cr(VI)-reducing bacterial community YEM001 from no-Cr(VI) polluted pond sedimental sludge by selectively growing it in Cr(VI)-containing media. This community could effectively reduce Cr(VI) in laboratory rich media containing different concentrations of Cr(VI), such as 61% reduction at 435 mg/L Cr(VI), 85% reduction at 355 mg/L Cr(VI), and complete reduction at 269 mg/L Cr(VI) in 93.5 h. It was also able to completely reduce 100 mg/L and 300 mg/L Cr(VI) in landfill leachate and natural sludge in 48 h, respectively. Optimal pH for Cr(VI) reduction of the YEM001 is between 7 and 8 and the best efficiency for Cr(VI) reduction occurs at 30 °C. Metagenomic data demonstrated that the YEM001 community was composed of multiple bacteria, including well-known Cr(VI)-reducing bacteria and non-Cr(VI)-reducing bacteria. Delftia, Comamonas, Alicycliphilus, Acidovorax, Bacillus, and Clostridioides account for 83% of total community abundance. The stability of the composition of the YEM001 community and its Cr(VI)-reducing activity allows for its application in bioremediation of environmental Cr(VI) pollution.
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http://dx.doi.org/10.1007/s11356-020-11863-0DOI Listing
January 2021

Consistent Estimation of Generalized Linear Models with High Dimensional Predictors via Stepwise Regression.

Entropy (Basel) 2020 Aug 31;22(9). Epub 2020 Aug 31.

Department of Biostatistics, University of Michigan, Ann Arbor, MI 48109, USA.

Predictive models play a central role in decision making. Penalized regression approaches, such as least absolute shrinkage and selection operator (LASSO), have been widely used to construct predictive models and explain the impacts of the selected predictors, but the estimates are typically biased. Moreover, when data are ultrahigh-dimensional, penalized regression is usable only after applying variable screening methods to downsize variables. We propose a stepwise procedure for fitting generalized linear models with ultrahigh dimensional predictors. Our procedure can provide a final model; control both false negatives and false positives; and yield consistent estimates, which are useful to gauge the actual effect size of risk factors. Simulations and applications to two clinical studies verify the utility of the method.
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http://dx.doi.org/10.3390/e22090965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597260PMC
August 2020

Development of a Broadly Protective, Self-Adjuvanting Subunit Vaccine to Prevent Infections by .

Front Immunol 2020 17;11:583008. Epub 2020 Nov 17.

Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence, KS, United States.

Infections caused by the opportunistic pathogen can be difficult to treat due to innate and acquired antibiotic resistance and this is exacerbated by the emergence of multi-drug resistant strains. Unfortunately, no licensed vaccine yet exists to prevent infections. Here we describe a novel subunit vaccine that targets the type III secretion system (T3SS). This vaccine is based on the novel antigen PaF (Pa Fusion), a fusion of the T3SS needle tip protein, PcrV, and the first of two translocator proteins, PopB. Additionally, PaF is made self-adjuvanting by the N-terminal fusion of the A1 subunit of the mucosal adjuvant double-mutant heat-labile enterotoxin (dmLT). Here we show that this triple fusion, designated L-PaF, can activate dendritic cells and elicits strong IgG and IgA titers in mice when administered intranasally. This self-adjuvanting vaccine expedites the clearance of from the lungs of challenged mice while stimulating host expression of IL-17A, which may be important for generating a protective immune response in humans. L-PaF's protective capacity was recapitulated in a rat pneumonia model, further supporting the efficacy of this novel fusion vaccine.
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http://dx.doi.org/10.3389/fimmu.2020.583008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705240PMC
November 2020

Synthesis of High-Water-Resistance Lignin-Phenol Resin Adhesive with Furfural as a Crosslinking Agent.

Polymers (Basel) 2020 Nov 27;12(12). Epub 2020 Nov 27.

School of Resources, Environment and Materials, Guangxi University, No. 100 Daxue East Road, Nanning 530004, China.

In this study, furfural was used as a crosslinking agent to enhance the water resistance of lignin-phenol-formaldehyde (LPF) resin. The effect of the furfural content on the physicochemical properties of the adhesives was explored, and the possible synthesis mechanism of the furfural-modified lignin-phenol-formaldehyde (LPFF) resin adhesives was investigated. Compared with the LPF adhesive, the LPFF adhesive with 15% furfural content and 50% lignin substituent exhibited outstanding properties in all considered aspects; it had a high wet shear strength (1.30 MPa), moderate solid content (54.51%), and low viscosity (128 mPa∙s), which were 38.0% higher, 3.6% higher, and 37.5% lower than those of the LPF adhesive. Analyses via nuclear magnetic resonance and Fourier transform infrared (FTIR) spectroscopy confirmed that the furfural content improved water resistance of the lignin-based adhesive; this improvement was due to the formation of new chemical bonds between furfural and lignin to construct a dense crosslinked network structure. In addition, the decrease in viscosity and the increase in solid content enabled the adhesive to better penetrate into the wood porous structure, showing stronger adhesion. Therefore, the LPFF adhesive has superior water resistance, high strength, and good thermal stability; thus, it has a great potential for industrial applications.
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http://dx.doi.org/10.3390/polym12122805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760341PMC
November 2020

miR-1246 shuttling from fibroblasts promotes colorectal cancer cell migration.

Neoplasma 2020 Nov 25. Epub 2020 Nov 25.

Department of Pathology, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Cancer-associated fibroblasts (CAFs) are the major constituents of the tumor microenvironment and promote cancer development via tumor-stromal interactions. The alteration of microRNA (miRNA) expression in fibroblasts can induce the phenotype conversion between normal fibroblasts and CAFs in certain tumor types. However, the mechanisms underlying phenotype conversion of fibroblasts in colorectal cancer (CRC) are largely unknown. Our study focuses on the role of miR-1246 in fibroblasts-CRC cells interaction. In this study, CCD-18Co colorectal fibroblasts were cultured in the conditioned medium (CM) derived from CRC cells to obtain the CAF phenotype. We found that the miR-1246 expression was upregulated in CAF-like fibroblasts compared with normal fibroblasts. miR-1246 secreted by cancer cells could be utilized by neighboring fibroblasts for CAF reprogramming. On the other hand, following secretion by CAF-like fibroblasts, miR-1246 was delivered into CRC cells and promoted cell migration via the activation of the Wnt/β-catenin signaling in CRC cells. Furthermore, high miR-1246 expression in CRC tissues was negatively associated with disease-free survival (DFS) for CRC patients. Taken together, our results reveal that miR-1246 can shuttle between CRC cells and fibroblasts. This study also indicates that targeting miR-1246 or blocking its transport from CAFs to CRC cells might represent a novel therapeutic approach in CRC treatment.
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http://dx.doi.org/10.4149/neo_2020_200924N1018DOI Listing
November 2020

Localized spin-orbit polaron in magnetic Weyl semimetal CoSnS.

Nat Commun 2020 Nov 5;11(1):5613. Epub 2020 Nov 5.

Beijing National Center for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.

The kagome lattice CoSnS exhibits the quintessential topological phenomena of a magnetic Weyl semimetal such as the chiral anomaly and Fermi-arc surface states. Probing its magnetic properties is crucial for understanding this correlated topological state. Here, using spin-polarized scanning tunneling microscopy/spectroscopy (STM/S) and non-contact atomic force microscopy (nc-AFM) combined with first-principle calculations, we report the discovery of localized spin-orbit polarons (SOPs) with three-fold rotation symmetry nucleated around single S-vacancies in CoSnS The SOPs carry a magnetic moment and a large diamagnetic orbital magnetization of a possible topological origin associated relating to the diamagnetic circulating current around the S-vacancy. Appreciable magneto-elastic coupling of the SOP is detected by nc-AFM and STM. Our findings suggest that the SOPs can enhance magnetism and more robust time-reversal-symmetry-breaking topological phenomena. Controlled engineering of the SOPs may pave the way toward practical applications in functional quantum devices.
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http://dx.doi.org/10.1038/s41467-020-19440-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644724PMC
November 2020

Identification of BAG5 from orange-spotted grouper (Epinephelus coioides) involved in viral infection.

Dev Comp Immunol 2021 Mar 1;116:103916. Epub 2020 Nov 1.

College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, PR China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524002, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

Bcl-2-associated athanogene 5 (BAG5) is a kind of molecular chaperone that can bind to the Bcl-2 and modulate cell survival. However, little is known about the functions of fish BAG5. In this study, we characterized a BAG5 homolog from orange-spotted grouper (Epinephelus coioides) gene (Ec-BAG5) and investigated its roles during viral infection. The Ec-BAG5 protein encoded 468 amino acids with four BAG domains, which shared high identities with reported BAG5. The highest transcriptional level of Ec-BAG5 was found in the peripheral blood lymphocyte (PBL). And the Ec-BAG5 expression were significantly up-regulated after red-spotted grouper nervous necrosis virus (RGNNV) or Lipopolysaccharide (LPS) stimulation in vitro. Furthermore, Ec-BAG5 overexpression could inhibited viral replication and the expression of viral genes (coat protein (CP) and RNA-dependent RNA polymerase (RdRp)). Also, overexpression of Ec-BAG5 significantly increased the expression of interferon pathway-related factors including interferon regulatory factor 3 (IRF3), interferon-stimulated gene 15 (ISG15), interferon-induced protein 35 (IFP35), myxovirus resistance gene 1 (Mx1) and inflammatory-related factors including tumor necrosis factor receptor-associated factor 6 (TRAF6), tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), as well as the activities of NF-κB, ISRE and IFN-1. These data indicate that Ec-BAG5 can affect viral infection through regulating the expression of IFN- and inflammation-related factors, which provide useful information to better understand the immune response against viral infection.
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http://dx.doi.org/10.1016/j.dci.2020.103916DOI Listing
March 2021

Guidance-oriented advanced curve speed warning system in a connected vehicle environment.

Accid Anal Prev 2020 Dec 28;148:105801. Epub 2020 Oct 28.

Center for Transportation Innovation, Department of Civil and Environmental Engineering, Louisville, University of Louisville, KY, 40292, USA. Electronic address:

Connected Vehicles (CV) technology has been used to address safety issues on highway horizontal curves. Existing curve warning systems are either using curve warning signs or providing drivers with an in-vehicle curve warning message in advance, allowing drivers to adjust their speed prior to the vehicle entering the curve. In practice, drivers might be compliant before entering the curve but may pick up the speed in the curve. Therefore, it remains a problem that existing curve warning systems are not able to guide drivers by providing necessary speed warnings through the entire course of approaching, entering, navigating, and leaving horizontal curves. Therefore, the objective of this study is to improve curve speed compliance by proposing a guidance-oriented Advanced Curve Speed Warning system (Advanced-CSW) with a focus on providing guided curve speed messages throughout the horizontal curves. The Advanced-CSW system is based on Dedicated Short-Range Communication (DSRC) enabling vehicle-infrastructure (V2I) communication. Anytime the vehicle is speeding, the guided message will be displayed until the vehicle's speed is within compliant range. Drivers who use the Advanced-CSW can receive multiple guided messages from the in-vehicle heads-up display through the entire course of navigating through horizontal curves. Thirty participants are recruited to perform the driving experiment on the simulator of driving through a series of horizontal curves under various geometric, roadway and traffic conditions. These conditions include different curve severity, illumination, and pavement wetness levels. The Advanced-CSW system's performance was evaluated in terms of the speed difference, which measures the gap between the in-curve mean speed and curve advisory speed. The results were compared with the performance of speed difference by driving with CSW or CSO through the entire curve. The experiment data was modeled using the mixed linear model with random effects, which includes the individual's driving behavior. In summary, when male drivers navigate through the horizontal curves under different curve speed warning systems, their speed compliance is significantly increased with continuous and guided messages provided in comparison with the speed compliance under the one-time curve speed warning message and the curve sign only. Female drivers improve their speed compliance in the curve by using curve signs only comparing to using one-time curve speed warning message or continuous guided curve speed warning messages. Also, male drivers' speed differences by using the guided system are significantly reduced by 6.53∼7.68 mi/h compared to driving with curve signs only or one-time curve speed warning message. In addition, there is also a speed reduction of 1.81 mi/h if male drivers receiving continuous guided messages in the curve during the daytime than during the nighttime. The proposed adaptive system based on that is adaptive to the vehicle's real-time speed and location by providing a new direction in designing effective curve warning systems. The speed-guided messages through the entire course of approaching, entering, navigating, and leaving horizontal curves can solve the current issue of speed incompliance by using the existing curve warning systems.
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http://dx.doi.org/10.1016/j.aap.2020.105801DOI Listing
December 2020

circPGAM1 enhances autophagy signaling during laryngocarcinoma drug resistance by regulating miR-376a.

Biochem Biophys Res Commun 2021 Jan 26;534:966-972. Epub 2020 Oct 26.

Department of Otorhinolaryngology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China. Electronic address:

circRNAs have been shown to be involved in cancer progression. It is unclear whether circPGAM1 exerts its effect on laryngocarcinoma drug resistance. In this study, we employed colony formation and MTT assay to determine colony number and cell viability under cisplatin treatment. TUNEL experiment was used to evaluate apoptosis of laryngocarcinoma cells in the presence of cisplatin. Xenograft tumor experiment was performed to assess in vivo tumor growth of SNU46 cells. We found that circPGAM1 enhanced colony formation and viability of SNU46 and M4E cells. In contrast, circPGAM1 caused attenuated cell apoptosis. Furthermore, we also confirmed that circPGAM1 played a key role in tumor growth in animal model and clinical patients. miR-376a was identified and proved to act as key effector for circPGAM1-mediated drug resistance. Finally, autophagy-related gene ATG2A was shown to rescue miR-376a-modulated drug resistance of laryngocarcinoma cells. Herein, we illuminate the role of circPGAM1 in laryngocarcinoma drug resistance, thereby facilitating development of targeted therapy for treating laryngocarcinoma.
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http://dx.doi.org/10.1016/j.bbrc.2020.10.063DOI Listing
January 2021

miR-224 targets and promotes cell migration and invasion in colorectal cancer.

3 Biotech 2020 Nov 24;10(11):485. Epub 2020 Oct 24.

Department of Pathology, School of Basic Medical Sciences, Fudan University, 131 Dong'an Road, Shanghai, 200032 Shanghai China.

Our study aims to investigate the impact of miR-224 on cell migration and invasion in colorectal cancer (CRC) as well as its molecular mechanisms. The results showed that miR-224 was significantly upregulated in CRC compared to normal tissues via the TCGA database. Overexpression of miR-224 promoted CRC cell migration and invasion, while inhibition of miR-224 demonstrated the opposite result via transwell assays. In addition, we found that was a target gene of miR-224 through the miRecords database and dual-luciferase assay, while western blot together with RT-qPCR showed that inhibition of miR-224 led to elevated expression in protein level but not in mRNA level, and also decreased the expression of β-catenin. In reference to the Human Protein Atlas, BTRC protein expression was higher in normal tissues than in CRC tissues. In conclusion, miR-224 regulates its target BTRC protein expression and its related Wnt/β-catenin pathway. Its impact on cell migration and invasion in CRC cells suggested that miR-224 could be a prospective therapeutic target for early-stage non-metastatic CRC.
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http://dx.doi.org/10.1007/s13205-020-02477-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585582PMC
November 2020

A Novel Heterostructure Based on RuMo Nanoalloys and N-doped Carbon as an Efficient Electrocatalyst for the Hydrogen Evolution Reaction.

Adv Mater 2020 Nov 15;32(46):e2005433. Epub 2020 Oct 15.

The Meso-Entropy Matter Lab, State Key Laboratory of Metal Matrix Composites, Shanghai Key Laboratory of Electrical Insulation and Thermal Aging, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, 800 Dongchuan RD., Shanghai, 200240, China.

Heterostructures exhibit considerable potential in the field of energy conversion due to their excellent interfacial charge states in tuning the electronic properties of different components to promote catalytic activity. However, the rational preparation of heterostructures with highly active heterosurfaces remains a challenge because of the difficulty in component tuning, morphology control, and active site determination. Herein, a novel heterostructure based on a combination of RuMo nanoalloys and hexagonal N-doped carbon nanosheets is designed and synthesized. In this protocol, metal-containing anions and layered double hydroxides are employed to control the components and morphology of heterostructures, respectively. Accordingly, the as-made RuMo-nanoalloys-embedded hexagonal porous carbon nanosheets are promising for the hydrogen evolution reaction (HER), resulting in an extremely small overpotential (18 mV), an ultralow Tafel slope (25 mV dec ), and a high turnover frequency (3.57 H s ) in alkaline media, outperforming current Ru-based electrocatalysts. First-principle calculations based on typical 2D N-doped carbon/RuMo nanoalloys heterostructures demonstrate that introducing N and Mo atoms into C and Ru lattices, respectively, triggers electron accumulation/depletion regions at the heterosurface and consequently reduces the energy barrier for the HER. This work presents a convenient method for rational fabrication of carbon-metal heterostructures for highly efficient electrocatalysis.
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http://dx.doi.org/10.1002/adma.202005433DOI Listing
November 2020

Orthogonalizing the Activity of Two Neural Units for 2D Cursor Movement Control.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:3046-3049

In the design of brain-machine interface (BMI), as the number of electrodes used to collect neural spike signals declines slowly, it is important to be able to decode with fewer units. We tried to train a monkey to control a cursor to perform a two-dimensional (2D) center-out task smoothly with spiking activities only from two units (direct units). At the same time, we studied how the direct units did change their tuning to the preferred direction during BMI training and tried to explore the underlying mechanism of how the monkey learned to control the cursor with their neural signals. In this study, we observed that both direct units slowly changed their preferred directions during BMI learning. Although the initial angles between the preferred directions of 3 pairs units are different, the angle between their preferred directions approached 90 degrees at the end of the training. Our results imply that BMI learning made the two units independent of each other. To our knowledge, it is the first time to demonstrate that only two units could be used to control a 2D cursor movements. Meanwhile, orthogonalizing the activities of two units driven by BMI learning in this study implies that the plasticity of the motor cortex is capable of providing an efficient strategy for motor control.
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http://dx.doi.org/10.1109/EMBC44109.2020.9175931DOI Listing
July 2020

Mapping QTL for agronomic traits under two levels of salt stress in a new constructed RIL wheat population.

Theor Appl Genet 2021 Jan 29;134(1):171-189. Epub 2020 Sep 29.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

Key Message: QTL for 15 agronomic traits under two levels of salt stress in dry salinity field were mapped in a new constructed RIL population utilizing a Wheat55K SNP array. Furthermore, eight QTL were validated in a collected natural population. Soil salinity is one of the major abiotic stresses causing serious impact on crop growth, development and yield. As one of the three most important crops in the world, bread wheat (Triticum aestivum L.) is severely affected by salinity, too. In this study, an F recombinant inbred line (RIL) population derived from a cross between high-yield wheat cultivar Zhongmai 175 and salt-tolerant cultivar Xiaoyan 60 was constructed. The adult stage performances of the RIL population and their parent lines under low and high levels of salt stress were evaluated for three consecutive growing seasons. Utilizing a Wheat55K SNP array, a high-density genetic linkage map spinning 3250.71 cM was constructed. QTL mapping showed that 90 stable QTL for 15 traits were detected, and they were distributed on all wheat chromosomes except 4D, 6B and 7D. These QTL individually explained 2.34-32.43% of the phenotypic variation with LOD values ranging from 2.68 to 47.15. It was found that four QTL clusters were located on chromosomes 2D, 3D, 4B and 6A, respectively. Notably, eight QTL from the QTL clusters were validated in a collected natural population. Among them, QPh-4B was deduced to be an allele of Rht-B1. In addition, three kompetitive allele-specific PCR (KASP) markers derived from SNPs were successfully designed for three QTL clusters. This study provides an important base for salt-tolerant QTL (gene) cloning in wheat, and the markers, especially the KASP markers, will be useful for marker-assisted selection in salt-tolerant wheat breeding.
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http://dx.doi.org/10.1007/s00122-020-03689-8DOI Listing
January 2021

Epidemic characteristics and drug resistance of tuberculosis in North China.

Heliyon 2020 Sep 16;6(9):e04945. Epub 2020 Sep 16.

Infectious Disease Department, First Affiliated Hospital of Harbin Medical University, 150001, China.

To analyze the epidemiological characteristics and drug resistance of tuberculosis (TB) patients in northern China. The epidemiological characteristics of 620 patients with tuberculosis from 2014 to 2016 were analyzed, including gender, age, occupation, education, income, place of residence and time distribution. 148 strains were identified as infection, 46 of 148 strains were identified as resistant strains, and among of which 73.91% of mono-resistance rate, 17.39% of poly-resistance rate, and 8.70% of multidrug resistant rate. Most of the patients were male, farmers, and the age of between 40 and 60, primary education background, income of 5000-10000RMB/year and newly diagnosed patients. The resistance rates of the four first line anti-tuberculosis drugs ranked as S (streptomycin)>R (rifampicin)>H (isoniazid)>E (ethambutol). 12 drug resistance spectrums were found, among them, mono-resistance was mainly concentrated in S (45.65%), poly-resistance was mainly concentrated in H + S (8.70%), and multidrug resistance was mainly concentrated in H + R (4.35%). Therefore, middle-aged people, male, farmers, education for elementary or junior high school, and new patients with incomes not exceeding 10000RMB/year will be the key population for prevention and control in the future and the main drug-resistant population. This is particularly relevant for controlling infection sources, community prevention and control, and drug resistance treatment.
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http://dx.doi.org/10.1016/j.heliyon.2020.e04945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502568PMC
September 2020

A New Therapy for Vitiligo Using Fire Needles: A Systematic Review of Evidence from 3618 Subjects.

Evid Based Complement Alternat Med 2020 27;2020:8492097. Epub 2020 Aug 27.

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

Introduction: Fire needle therapy has been reported as an effective treatment for vitiligo. However, current clinical evidence has not been systematically evaluated. The aim of this study was to determine whether fire needle therapy is effective and safe for treating vitiligo.

Methods: Seven databases were searched until October 2019 for randomized controlled trials on fire needle therapy, with and without conventional treatments, versus any type of conventional therapy for treating vitiligo. The RevMan 5.3.5 software was used to perform meta-analysis of the included studies.

Results: Forty-seven trials comprising 3618 patients were included. Fire needle combined with conventional vitiligo treatments had a higher efficacy (risk ratio (RR): 1.55, 95% confidence interval (CI): 1.46-1.65, < 0.00001 and RR: 1.41, 95% CI: 1.24-1.61, < 0.00001, respectively) and a greater effect on restoring the color of the area of the skin lesion (mean difference (MD): 3.40, 95% CI: 2.11-4.69, < 0.00001), increasing the pigment point of vitiligo (MD: 0.83, 95% CI: 0.54-1.13, < 0.00001) and improving the cytokine level (MD: 8.10, 95% CI: 6.94-9.27, < 0.00001) and effectual time (MD: -4.76, 95% CI: -7.33 to -2.19, =0.0003) than traditional methods. Limb lesions (RR: 1.60, 95% CI: 1.31-1.95, < 0.00001) were more effectively treated when the treatments included fire needles, whereas the therapeutic effect of fire needles on either the head and neck (RR: 1.13, 95% CI: 0.78-1.64, =0.52) or torso lesions (RR: 1.22, 95% CI: 0.82-1.81, =0.33) was not significantly different compared to that without fire needles. No statistically significant differences in adverse effects (RR: 1.15, 95% CI: 0.89-1.49, =0.28) and recurrence rates (RR: 0.90, 95% CI: 0.17-4.92, =0.91) during the follow-up period were observed between treatment with and without fire needles.

Conclusions: Fire needle therapy combined with other conventional treatments is useful in treating vitiligo. Further studies with larger sample sizes should be performed to make a conclusive judgment. This trial is registered with CRD42018094918.
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http://dx.doi.org/10.1155/2020/8492097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474359PMC
August 2020

Development of a Smartphone-Based Fluorescent Immunochromatographic Assay Strip Reader.

Sensors (Basel) 2020 Aug 13;20(16). Epub 2020 Aug 13.

Key Lab of Medical Instrumentation & Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China.

Fluorescence immunochromatographic assay (FICA) is a rapid immunoassay technique that has the characteristics of high precision and sensitivity. Although image FICA strip readers have the advantages of high portability and easy operation, the use of high-precision complementary metal oxide semiconductor (CMOS) image sensors leads to an increase in overall cost. Considering the popularity of CMOS image sensors in smartphones and their powerful processing functions, this work developed a smartphone-based FICA strip reader. An optical module suitable for the test strips with different fluorescent markers was designed by replacing the excitation light source and the light filter. An android smartphone was used for image acquisition and image denoising. Then, the test and control lines of the test strip image were recognized by the sliding window algorithm. Finally, the characteristic value of the strip image was calculated. A linear detection range from 10 to 5000 mIU/mL ( = 0.95) was obtained for human chorionic gonadotrophin with the maximum relative error less than 9.41%, and a linear detection range from 5 to 4000 pg/mL ( = 0.99) was obtained for aflatoxin B1, with the maximum relative error less than 12.71%. Therefore, the smartphone-based FICA strip reader had high portability, versatility, and accuracy.
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http://dx.doi.org/10.3390/s20164521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471973PMC
August 2020