Publications by authors named "Qi Zhang"

4,406 Publications

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sp. nov., a facultative anaerobic bacterium isolated from mangrove sediment.

Int J Syst Evol Microbiol 2021 Aug;71(8)

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.

Two Gram-stain-negative, motile, facultatively anaerobic, rod-shaped strains, FJAT-53720 and FJAT-53726, were isolated from rhizosphere sediment of plant . Phylogenetic analysis based on their 16S rRNA gene sequences revealed that strains FJAT-53720 and FJAT-53726 were affiliated to the genus (forming an independent cluster) with the highest sequence similarity to the type strain of . Optimum growth of both strains was observed at 30 °C and pH 7. The respiratory quinones were Q-7, Q-8 and MK-7. The polar lipid profile included phosphatidylmethyl ethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminophospholipid and four unknown phospholipids. The major fatty acids of strains FJAT-53720 and FJAT-53726 were iso-C C 8 and summed feature 3. The genomic DNA G+C content of strain FJAT-53720 was 55.6 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between FJAT-53720 and FJAT-53726 were 97.4 and 77.9 %, confirming that they were similar species and hence FJAT-53720 was selected for further analysis. The ANI and dDDH values between FJAT-53720 and other members of the genus were below the cut-off level (95-96 %; 70 %) for species delineation. Based on the above results, FJAT-53720 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-53720 (=KCTC 82444=GDMCC 1.2207).
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http://dx.doi.org/10.1099/ijsem.0.004940DOI Listing
August 2021

Enantioselective metabolomic modulations in Arabidopsis thaliana leaf induced by the herbicide dichlorprop.

Sci Total Environ 2021 Jul 15;797:149015. Epub 2021 Jul 15.

College of Environment, Zhejiang University of Technology, Hangzhou 310014, PR China.

Over 40% of herbicides used today are chiral. Dichlorprop (2, 4-DCPP) is a widely used typical broad-spectrum chiral aryloxyphenoxy propionic acid (AOPP) herbicide. However, the molecular mechanism of the enantioselectivity of DCPP enantiomers (S-DCPP and R-DCPP) and their effects on non-target organisms are remain unclear. In the present study, the model plant Arabidopsis thaliana was treated by DCPP enantiomers to directly reveal the effects of DCPP enantiomers on plant growth, as well as metabolic profile. Results showed that the enantioselectivity embodied in that R-DCPP treatment led to the decrease of shoot weight, the significantly variation on morphology of shoot and root, oxidative damage, et al., while the plant morphology also changes to a certain extent associated oxidative damage after treated by S-DCPP. By using metabolomic analysis, it was found that R-DCPP had significant effects on A. thaliana leaf metabolism, including lactose metabolism, starch and sucrose metabolism, TCA cycle, fatty acid biosynthesis pathway and pentose phosphate pathway, and accumulated a lot of antioxidants in plant leaves, while the amino acids and some terpenoids increased in S-DCPP group. Our study provides a new direction to explore the relationship between chiral herbicides on leaf metabolism, and the effect of this relationship on the plant growth.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149015DOI Listing
July 2021

Structurally Diverse Synthesis of Five-, Six-, and Seven-Membered Benzosultams through Electrochemical Cyclization.

Org Lett 2021 Aug 3. Epub 2021 Aug 3.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Xi'an, Shaanxi 710119, P. R. China.

We have developed a metal- and oxidant-free approach to structurally diverse synthesis of benzosultams from aryl sulfonamides through an electrochemical cyclization. Upon variation of the substituent on aryl sulfonamides, five-, six-, and seven-membered benzosultams were efficiently assembled in an atom- and resource-economic manner. The generality of the process is demonstrated by the formation of five- to seven-membered cyclic products from 42 substrates bearing substituents with different electronic effects and steric hindrance.
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http://dx.doi.org/10.1021/acs.orglett.1c02128DOI Listing
August 2021

Robustness and accuracy improvement of data processing with 2D neural networks for transient absorption dynamics.

Phys Chem Chem Phys 2021 Aug 2. Epub 2021 Aug 2.

Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, P. R. China.

To achieve the goal of efficiently analyzing transient absorption spectra without arbitrary assumption and to overcome the limitations of conventional methods in fitting ability and highly noised backgrounds, it is essential to develop new tools to achieve more accurate and robust prediction based on the intrinsic properties of a spectrum even under strong noise. In this work, Lasso regression and neural network were combined to achieve an effective fitting. Compared to the conventional global fitting method, our network could automatically determine the exponential form on each wave unit, in which the accuracy was as high as 97%. Thereafter, the lifetime with the corresponding amplitude ratio could be easily predicted by the neural network on each wave unit. This kind of prediction is difficult to achieve by global fitting due to the limitation of computational resources. Furthermore, more accurate fitting even under weak signals could be achieved for the mean square error (MSE) decreasing by more than 100 times on average compared to conventional global fitting methods. Attributed to its improved accuracy and robustness, our developed algorithm could be readily applied to analyze time-resolved transient spectra.
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http://dx.doi.org/10.1039/d1cp02521jDOI Listing
August 2021

Association of systemic inflammation with survival in patients with cancer cachexia: results from a multicentre cohort study.

J Cachexia Sarcopenia Muscle 2021 Aug 2. Epub 2021 Aug 2.

Department of Gastrointestinal Surgery/Department of Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Although systemic inflammation is an important feature of the cancer cachexia, studies on the association between systemic inflammation and prognostic of cancer cachexia are limited. The objective of this study is to evaluate whether the neutrophil-to-lymphocyte ratio (NLR) is associated with outcome and quality of life for patients with cancer cachexia and investigated any interaction between NLR and the clinical parameters.

Methods: This is a multicentre cohort study of 2612 cancer patients suffering from cachexia diagnosed between June 2012 and December 2019. The main parameters measured were overall survival (OS) time and all-cause mortality. The association between NLR and all-cause mortality was evaluated using hazard ratios (HRs) and the restricted cubic spline model with a two-sided P-value. Optimal stratification was used to solve threshold points. We also evaluated the cross-classification of NLR for each variable of survival.

Results: Of the 2612 participants diagnosed with cancer cachexia, 1533 (58.7%) were male, and the mean (SD) age was 58.7 (11.7) years. Over a median follow-up of 4.5 years, we observed 1189 deaths. The overall mortality rate for patients with cancer cachexia during the first 12 months was 30.2% (95%CI: 28.4%-32.0%), resulting in a rate of 226.07 events per 1000 patient-years. An increase in NLR had an inverted L-shaped dose-response association with all-cause mortality. The optimal cut-off point for NLR as a predictor of mortality in cancer patients with cachexia was 3.5. An NLR of 3.5 or greater could independently predict OS (HR, 1.51, 95%CI: 1.33-1.71). These associations were consistent across subtypes of cancer. Several potential effect modifiers were identified including gender, BMI, tumour type, KPS score and albumin in content. Increasing NLRs were independently associated with a worsening in the majority of EORTC QLQ-C30 domains. Elevated baseline NLR was associated with low response and poor survival in patients treated with immunotherapy.

Conclusions: The baseline NLR status was found to be a significant negative prognostic biomarker for patients with cachexia; this effect was independent of other known prognostic factors.
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http://dx.doi.org/10.1002/jcsm.12761DOI Listing
August 2021

Siglec-15 Promotes Tumor Progression in Osteosarcoma DUSP1/MAPK Pathway.

Front Oncol 2021 16;11:710689. Epub 2021 Jul 16.

Department of Orthopedic Research Center, The Third Hospital of Hebei Medical University, Shijiazhuang, China.

Recurrence and metastasis are important features of osteosarcoma (OS) that cause its poor prognosis. Aberrant expression of Sialic acid-binding immunoglobulin-like lectin 15 (Siglec-15) has been reported in various kinds of cancers. However, the expression and function of Siglec-15 in OS remain unclear. In cultured OS cells (143B cells and MNNG/HOS cells) and their xenograft mouse models, we found that downregulation of Siglec-15 could inhibit the proliferation, migration and invasion of by inducing epithelial-mesenchymal transition (EMT) and . Conversely, Siglec-15 overexpression promoted the growth, migration and invasion of OS cells in a significant manner. Then, we screened a number of differentially expressed genes (DEGs) between Siglec-15-knockdown group and control group by RNA-Seq assay. Among these DEGs, we found that dual-specificity phosphatase 1 (DUSP1/MKP1) was significantly downregulated after Siglec-15 silencing. We investigated the DUSP1 functions in influencing OS cells' biology, and found that the proliferation, migration and invasion of OS cells were promoted by overexpressing DUSP1 and crucially, the proliferation, migration and invasion of Siglec-15-knockdown OS cells were rescued by overexpressing DUSP1. Mechanically, we further showed that DUSP1-mediated inhibition of p38/MAPK and JNK/MAPK expression was attenuated when Siglec-15 expression was inhibited, suggesting that Siglec-15 promotes the malignant progression of OS cells by suppressing DUSP1-mediated suppression of the MAPK pathway. Moreover, we showed that both Siglec-15 and DUSP1 were highly expressed in human OS tissues by immunohistochemistry. High Siglec-15 expression was associated with OS lung metastasis, and high DUSP1 expression was associated with the high Enneking stage. Kaplan-Meier analysis indicated that high expression of Siglec-15 could predict poor prognosis of OS patients. Altogether, these results showed that Siglec-15 expression promoted OS development and progression by activating DUSP1 and might be a novel target in OS treatment.
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http://dx.doi.org/10.3389/fonc.2021.710689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322944PMC
July 2021

Clinical and Genetic Predictive Models for the Prediction of Pathological Complete Response to Optimize the Effectiveness for Trastuzumab Based Chemotherapy.

Front Oncol 2021 15;11:592393. Epub 2021 Jul 15.

Department of Breast Surgery, Shanghai Cancer Center, Fudan University, Shanghai, China and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Background: Trastuzumab shows excellent benefits for HER2+ breast cancer patients, although 20% treated remain unresponsive. We conducted a retrospective cohort study to optimize neoadjuvant chemotherapy and trastuzumab treatment in HER2+ breast cancer patients.

Methods: Six hundred patients were analyzed to identify clinical characteristics of those not achieving a pathological complete response (pCR) to develop a clinical predictive model. Available RNA sequence data was also reviewed to develop a genetic model for pCR.

Results: The pCR rate was 39.8% and pCR was associated with superior disease free survival and overall survival. ER negativity and PR negativity, higher HER2 IHC scores, higher Ki-67, and trastuzumab use were associated with improved pCR. Weekly paclitaxel and carboplatin had the highest pCR rate (46.70%) and the anthracycline+taxanes regimen had the lowest rate (11.11%). Four published GEO datasets were analyzed and a 10-gene model and immune signature for pCR were developed. Non-pCR patients were ERPR and had a lower immune signature and gene model score. Hormone receptor status and immune signatures were independent predictive factors of pCR.

Conclusion: Hormone receptor status and a 10-gene model could predict pCR independently and may be applied for patient selection and drug effectiveness optimization.
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http://dx.doi.org/10.3389/fonc.2021.592393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319743PMC
July 2021

Derivation and Verification of the Relationship between Ablation Index and Baseline Impedance.

Cardiol Res Pract 2021 12;2021:5574125. Epub 2021 Jul 12.

Department of Cardiology, General Hospital of Northern Theater Command, Shenyang 110016, China.

Objective: To explore the quantitative adjustment of ablation index (AI) under different baseline impedance to achieve similar lesion dimensions.

Methods: (1) Keeping the AIs relatively constant, the lesion dimensions in different baseline impedances were studied. (2) According to Joule's law,  =  RT, keeping the current () unchanged, the powers corresponding to different baseline impedances can be obtained. Under different baseline impedances and corresponding powers, the swine hearts were ablated for 30 s in simulated human circumstances. The baseline impedances, the lesion dimensions, and AIs were recorded. And the derivation of empirical formula was achieved according to the AIs and baseline impedance values in similar lesions dimension. (3) Basic AI and baseline impedance (AI/ ) were set as 400/120 Ω in the common AI groups and 550/120 Ω in the high AI groups, AI values in different baseline impedances were calculated using the empirical formula, and the corresponding lesion dimensions were measured to verify this formula.

Results: (1) Higher baseline impedances were related to smaller lesion dimensions at similar AIs. (2) The lesion dimensions were roughly the same after modulating the baseline impedance and power to keep the electric current relatively constant. The relationship between AI and fitted with experimental data is AI = 1.9933 + 203.61 ( = 0.9649), and the formula derived is ΔAI = (AI - 203)/  × Δ. (3) Under the guidance of the empirical formula, there was no significant difference in lesion dimensions between the standard group and the formula guiding groups when AI = 400, but there was a shrinking tendence when AI > 700.

Conclusion: The lesion depths are negatively correlated with baseline impedance at a certain AI. The relationship between baseline impedance and AI is "ΔAI = (AI - 203)/  × Δ". It is verified that when the AI is not too high, the empirical formula can be used to guide the quantitative adjustment of AIs at different baseline impedance, and the lesion depths achieved are roughly the same.
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http://dx.doi.org/10.1155/2021/5574125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289601PMC
July 2021

Use of Deep-Learning Genomics to Discriminate Healthy Individuals from Those with Alzheimer's Disease or Mild Cognitive Impairment.

Behav Neurol 2021 14;2021:3359103. Epub 2021 Jul 14.

Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Objectives: Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder and the most common form of dementia in the elderly. Certain genes have been identified as important clinical risk factors for AD, and technological advances in genomic research, such as genome-wide association studies (GWAS), allow for analysis of polymorphisms and have been widely applied to studies of AD. However, shortcomings of GWAS include sensitivity to sample size and hereditary deletions, which result in low classification and predictive accuracy. Therefore, this paper proposes a novel deep-learning genomics approach and applies it to multitasking classification of AD progression, with the goal of identifying novel genetic biomarkers overlooked by traditional GWAS analysis.

Methods: In this study, we selected genotype data from 1461 subjects enrolled in the Alzheimer's Disease Neuroimaging Initiative, including 622 AD, 473 mild cognitive impairment (MCI), and 366 healthy control (HC) subjects. The proposed deep-learning genomics (DLG) approach consists of three steps: quality control, coding of single-nucleotide polymorphisms, and classification. The ResNet framework was used for the DLG model, and the results were compared with classifications by simple convolutional neural network structure. All data were randomly assigned to one training/validation group and one test group at a ratio of 9 : 1. And fivefold cross-validation was used.

Results: We compared classification results from the DLG model to those from traditional GWAS analysis among the three groups. For the AD and HC groups, the accuracy, sensitivity, and specificity of classification were, respectively, 98.78 ± 1.50%, 98.39% ± 2.50%, and 99.44% ± 1.11% using the DLG model, while 71.38% ± 0.63%, 63.13% ± 2.87%, and 85.59% ± 6.66% using traditional GWAS. Similar results were obtained from the other two intergroup classifications.

Conclusion: The DLG model can achieve higher accuracy and sensitivity when applied to progression of AD. More importantly, we discovered several novel genetic biomarkers of AD progression, including rs6311 and rs6313 in HTR2A, rs1354269 in NAV2, and rs690705 in RFC3. The roles of these novel loci in AD should be explored in future research.
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http://dx.doi.org/10.1155/2021/3359103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298161PMC
July 2021

Diabetic Peripheral Neuropathy Affects Pinch Strength and Hand Dexterity in Elderly Patients.

Neural Plast 2021 20;2021:9959103. Epub 2021 Jul 20.

Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, China.

Objective: Diabetic peripheral neuropathy (DPN) is one of the most common chronic complications of diabetes, leading to disability and decreased quality of life. In past research and clinical studies, the lower limb function of DPN patients was often the principal subject of research, with little attention given to the upper limb and hand. Our goal was to assess and compare hand function between elderly diabetic patients with DPN and without DPN.

Methods: A total of 52 diabetic patients were registered and underwent hand function assessments and electrodiagnostic tests. Dynamometer, pinch meter, Semmes Weinstein monofilaments, and the Purdue Pegboard Test (PPT) were used to assess the patients' grip strength, pinch strength, tactile sensory threshold, and hand dexterity.

Results: Compared with the non-DPN group, the elderly DPN group showed worse thumb-middle fingertip pinch strength and thumb-little fingertip pinch strength in the dominant hand (3.50 (2.50, 4.25) vs. 4.50 (3.00, 5.00), = 0.019; 1.50 (1.00, 2.00) vs. 2.50 (2.00, 3.00), < 0.001); the elderly DPN group displayed worse thumb-middle fingertip pinch strength, thumb-ring fingertip pinch strength, and thumb-little fingertip pinch strength in the nondominant hand (3.50 (2.00, 4.50) vs. 4.00 (3.00, 5.00), = 0.013; 2.50 (1.25, 3.00) vs. 3.00 (2.50, 3.50), = 0.033; 1.00 (0.75, 2.25) vs. 2.50 (2.00, 2.50), < 0.001). The elderly DPN group scored lower than the non-DPN group on the PPT test of assembly (13.96 ± 5.18 vs. 16.96 ± 4.61, = 2.212, = 0.032).

Conclusion: Motor function limitation is the principal hand dysfunction in elderly patients with DPN, which is mainly manifested as a decline in fingertip pinch strength and a decrease in hand dexterity. This trial is registered with Clinical Trial Registry no. ChiCTR1900025358.
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http://dx.doi.org/10.1155/2021/9959103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318756PMC
July 2021

Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action.

Phytomedicine 2021 Jul 9;91:153650. Epub 2021 Jul 9.

Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Guangzhou 510006, China; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education, Guangzhou 510006, China; Institute of Chinese Medicine, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou 510006, China. Electronic address:

Background: Diabetic nephropathy (DN) is associated with renal interstitial injury and fibrosis. Our previous study showed that andrographolide protected against the progression of DN and high glucose (HG)-induced mesangial dysfunction. However, the protective effects of andrographolide on renal tubular epithelial cells have not been fully elucidated.

Purpose: To determine the protective effects of andrographolide on renal tubular damage and explore the underlying mechanism.

Study Design: Human tubular epithelial cells (HK-2 cells) were treated with andrographolide (5 and 10 μM) under HG conditions. Diabetic mice were treated with andrographolide (i.p. 2 and 4 mg/kg, twice per week).

Methods: Western blotting, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence and flow cytometry were used to analyze the effects of andrographolide on renal tubular injury and fibrotic mechanisms in HK-2 cells. The protective effects of andrographolide against renal tubulointerstitial injury and fibrosis were investigated in diabetic mice fed a high-fat diet (HFD). Renal interstitial tissue was collected at sacrifice for immunohistochemistry, immunofluorescence analysis, RT-PCR and Western blotting to analyze the effects of andrographolide on renal tubular injury and fibrosis.

Results: In vitro assay results indicated that andrographolide (5 and 10 μM) effectively inhibited HG-induced apoptosis, epithelial-mesenchymal transition (EMT) and collagen deposition in HK-2 cells. Mechanistically, HG stimulated mitochondrial reactive oxygen species (mtROS)-mediated NOD-like receptor family and pyrin domain-containing protein 3 (NLRP3) inflammasome activation and EMT in tubular epithelial cells, and andrographolide (5 and 10 μM) inhibited these effects by ameliorating mitochondrial dysfunction. In vivo, treatment with andrographolide (2 and 4 mg/kg) inhibited renal tubular cell apoptosis, EMT and tubulointerstitial fibrosis, mitochondrial dysfunction and NLRP3 inflammasome activation in diabetic mice.

Conclusion: Andrographolide (5 and 10 μM) prevents HG-induced renal tubular cell damage, and andrographolide (2 and 4 mg/kg) protects against the progression of diabetic tubular injury and fibrosis in mice by suppressing mitochondrial dysfunction and NLRP3 inflammasome activation.
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http://dx.doi.org/10.1016/j.phymed.2021.153650DOI Listing
July 2021

Association between preconceptional air pollution exposure and medical purposes for selective termination of pregnancy.

Environ Res 2021 Jul 28;202:111743. Epub 2021 Jul 28.

Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, 230032, China; Department of Teaching Center for Preventive Medicine, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, 230032, China. Electronic address:

Background: Exposure to air pollutants is associated with adverse pregnancy outcomes. But evidence on the effects of preconceptional air pollution exposure on the risk of termination of pregnancy (TOP) caused by pregnancy losses and congenital malformations is lacking.

Methods: The distributed lag nonlinear model (DLNM) was used to evaluate the impact of short-term air pollutants exposure on the risk of TOP. Stratified analyses by age (<35 years old, ≥ 35 years old) and season (warm season, cold season) were further conducted. Relative risk (RR) and 95 % confidential interval (95 % CI) were calculated for per interquartile range (IQR) increment in air pollutants during the study period.

Results: PM, PM, and O exposure were significantly associated with elevated risk of TOP. The risk of TOP was associated with PM exposure from lag11 to lag15 in the single-pollutant model, and the strongest association was observed at lag13 (RR = 1.021, 95%CI:1.002-1.040). PM exposure from lag10 to lag15 was associated with increased TOP risk, with the corresponding peak association being at lag13 (RR = 1.020, 95%CI: 1.004-1.037). For O, the single-day lag association appeared to be statistically significant from lag26 to lag27, with the highest RR of TOP cases being at lag27 (RR = 1.044, 95%CI: 1.005-1.084). Similar results were observed for pregnancy losses (PL). However, no significantly association between air pollution exposure and the risk of congenital malformations (CM) was found in this study. Stratified analyses showed that pregnant women with more advanced ages were more susceptible to PM, PM, and O exposure. The effect of PM exposure was statistically significant in cold season subgroups.

Conclusion: The findings suggest that exposure to PM, PM, and O before pregnancy are associated with the risk of TOP in Lu'an, China, reflecting the significance of preconceptional environmental exposure in the development of adverse pregnancy outcomes.
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http://dx.doi.org/10.1016/j.envres.2021.111743DOI Listing
July 2021

Relationship of soil pH value and soil Pb bio-availability and Pb enrichment in tea leaves.

J Sci Food Agric 2021 Jul 30. Epub 2021 Jul 30.

Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, 35002, China.

Background: Pb is not an essential element for the growth of tea trees, but it is an important index for evaluating the quality and safety of tea. Pb is a sensitive metal to pH. Exploring the change trend of soil Pb and enrichment coefficient of Pb in tea leaves affected by soil acidification is significant for tea planting and tea quality safety control.

Results: The 37.57% of 364 tea plantations in Anxi county of China were soil acidification that soil pH value < 4.5. The soil total Pb content and Pb content of tea leaves met the requirements stipulated in China. The soil available Pb content and Pb content in tea leaves were both significant negative correlation with soil pH value, and increased with the decrease of soil pH value. The soil available Pb content had a significant positive correlation with soil total Pb content. However, the soil total Pb content had a no significant correlation with soil pH value. More, the soil Pb bio-availability coefficient and the Pb enrichment coefficient of tea leaves decreased with the increase of soil pH value.

Conslusion: More than a third of tea plantations in Anxi county had been acidified. The decrease of pH value leads to an increase in the bio-availability coefficient of soil Pb content and the enrichment coefficient of Pb content in tea leaves. The lower soil pH value resulted in the increase of the absorption and accumulation of Pb by tea trees, then increased Pb content in tea leaves. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11450DOI Listing
July 2021

Which Anthropometric Measurement is better for Predicting Survival of Patients with Cancer Cachexia?

Br J Nutr 2021 Jul 30:1-28. Epub 2021 Jul 30.

The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

No relevant studies have yet been conducted to explore which measurement can best predict the survival time of patients with cancer cachexia. This study aimed to identify an anthropometric measurement that could predict the 1-year survival of patients with cancer cachexia. We conducted a nested case-control study using data from a multicenter clinical investigation of cancer from 2013 to 2020. Cachexia was defined using the Fearon criteria. A total of 262 patients who survived less than 1 year and 262 patients who survived more than 1 year were included in this study. Six candidate variables were selected based on clinical experience and previous studies. Five variables; body mass index, mid-arm circumference, mid-arm muscle circumference, calf circumference, and triceps skin fold (TSF), were selected for inclusion in the multivariable model. In the conditional logistic regression analysis, TSF (p=0.014) was identified as a significant independent protective factor. A similar result was observed in all patients with cancer cachexia (n=3084). In addition, a significantly stronger positive association between TSF and the 1-year survival of patients with cancer cachexia was observed in participants aged >65 years (OR: 0.94; 95% CI: 0.89, 0.99) than in those aged ≤65 years (OR: 0.96; 95% CI: 0.93, 0.99; P-interaction =0.013) and in participants with no chronic disease (OR: 0.92; 95% CI: 0.87, 0.97) than in those with chronic disease (OR: 0.97; 95% CI: 0.94,1.00; P-interaction =0.049). According to this study, TSF might be a good anthropometric measurement for predicting 1-year survival in patients with cancer cachexia.
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http://dx.doi.org/10.1017/S0007114521002853DOI Listing
July 2021

Association between blood ethylene oxide levels and the risk of cardiovascular diseases in the general population.

Environ Sci Pollut Res Int 2021 Jul 28. Epub 2021 Jul 28.

Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210008, China.

Ethylene oxide (EtO) is a highly reactive organic compound that is mainly used as a sterilizing agent. However, to date, the effects of EtO on the cardiovascular system are not clear. We aimed to explore the association between blood EtO levels and the risk of cardiovascular disease (CVD) in the general US population. We obtained information on blood levels of EtO and CVD outcomes in 3,410 participants from the National Health and Nutritional Examination Survey (NHANES) 2013-2014 and 2015-2016. Logistic regression models were applied to calculate the odds ratios (ORs) and 95% confidence intervals (95% CIs) for the association between EtO and risk of all CVD as well as subtypes of CVD. Linear regression analyses were used to estimate the associations of EtO with potential mechanistic parameters of CVD, including blood pressure, blood lipid levels and inflammatory parameters. Higher blood levels of EtO were associated with an increased risk of all CVD (p for trend = 0.003), with an adjusted OR (95% CI) in the highest quartile of 1.94 (1.24, 3.02) compared with the lowest quartile as a reference. Higher concentrations of EtO were positively associated with the risk of angina (p for trend = 0.04) and heart attack (p for trend = 0.011). In addition, the concentration of EtO was positively associated with the levels of triglycerides, white blood cells, lymphocytes, monocytes, neutrophils and eosinophils (p = 0.003 for eosinophils and p < 0.001 for the others) and negatively associated with the level of high-density lipoprotein cholesterol (p < 0.001). We found that exposure to EtO was associated with angina, heart attack and all CVD in a large representative US population. Furthermore, EtO may induce CVD through the inflammatory response and abnormal fatty acid metabolism.
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http://dx.doi.org/10.1007/s11356-021-15572-0DOI Listing
July 2021

PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis.

Nat Commun 2021 07 28;12(1):4592. Epub 2021 Jul 28.

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC, Australia.

The polycomb repressive complex 2 (PRC2) is a histone methyltransferase that maintains cell identities. JARID2 is the only accessory subunit of PRC2 that known to trigger an allosteric activation of methyltransferase. Yet, this mechanism cannot be generalised to all PRC2 variants as, in vertebrates, JARID2 is mutually exclusive with most of the accessory subunits of PRC2. Here we provide functional and structural evidence that the vertebrate-specific PRC2 accessory subunit PALI1 emerged through a convergent evolution to mimic JARID2 at the molecular level. Mechanistically, PRC2 methylates PALI1 K1241, which then binds to the PRC2-regulatory subunit EED to allosterically activate PRC2. PALI1 K1241 is methylated in mouse and human cell lines and is essential for PALI1-induced allosteric activation of PRC2. High-resolution crystal structures revealed that PALI1 mimics the regulatory interactions formed between JARID2 and EED. Independently, PALI1 also facilitates DNA and nucleosome binding by PRC2. In acute myelogenous leukemia cells, overexpression of PALI1 leads to cell differentiation, with the phenotype altered by a separation-of-function PALI1 mutation, defective in allosteric activation and active in DNA binding. Collectively, we show that PALI1 facilitates catalysis and substrate binding by PRC2 and provide evidence that subunit-induced allosteric activation is a general property of holo-PRC2 complexes.
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http://dx.doi.org/10.1038/s41467-021-24866-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319299PMC
July 2021

Systematic thermodynamic analysis of apremilast polymorphs via solubility measurement with modeling: Mechanism evaluation through molecular simulation.

Eur J Pharm Sci 2021 Jul 25;165:105958. Epub 2021 Jul 25.

State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.

The polymorphism of apremilast has been investigated. Two polymorphs have been identified and characterized by differential scanning calorimeter, fourier transform infrared spectroscopy, and powder X-ray diffractometer. Solubilities of apremilast forms B and E in three binary solvents of methanol-water, acetonitrile-water, and acetonitrile-methanol have been measured using the static method at a temperature ranging from 288.15 K to 328.15 K under standard atmospheric pressure. Subsequently, the solubility data have been analyzed using the Wilson, NRTL, and UNIQUAC thermodynamic models, respectively. Furthermore, the Gibbs energy of solution and the radial distribution function have been calculated using the molecular simulation method to evaluate the dissolution mechanism. The Gibbs energy of solution reveals that the rank of solute-solvent interaction correlated well with solubility order in binary solvent mixtures, and the radial distribution function indicates that weakening of solvent-solvent interaction led to an increase in solubility.
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http://dx.doi.org/10.1016/j.ejps.2021.105958DOI Listing
July 2021

AbdomenCT-1K: Is Abdominal Organ Segmentation A Solved Problem.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 27;PP. Epub 2021 Jul 27.

With the unprecedented developments in deep learning, automatic segmentation of main abdominal organs seems to be a solved problem as state-of-the-art (SOTA) methods have achieved comparable results with inter-rater variability on many benchmark datasets. However, most of the existing abdominal datasets only contain single-center, single-phase, single-vendor, or single-disease cases, and it is unclear whether the excellent performance can generalize on diverse datasets. This paper presents a large and diverse abdominal CT organ segmentation dataset, termed AbdomenCT-1K, with more than 1000 (1K) CT scans from 12 medical centers, including multi-phase, multi-vendor, and multi-disease cases. Furthermore, we conduct a large-scale study for liver, kidney, spleen, and pancreas segmentation and reveal the unsolved segmentation problems of the SOTA methods, such as the limited generalization ability on distinct medical centers, phases, and unseen diseases. To advance the unsolved problems, we further build four organ segmentation benchmarks for fully supervised, semi-supervised, weakly supervised, and continual learning, which are currently challenging and active research topics. Accordingly, we develop a simple and effective method for each benchmark, which can be used as out-of-the-box methods and strong baselines. We believe the AbdomenCT-1K dataset will promote future in-depth research towards clinical applicable abdominal organ segmentation methods.
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http://dx.doi.org/10.1109/TPAMI.2021.3100536DOI Listing
July 2021

Achieving Band Gap Reduction and Carrier Lifetime Enhancement in Metal Halide Perovskites via Mechanical Stretching.

J Phys Chem Lett 2021 Aug 26;12(30):7207-7212. Epub 2021 Jul 26.

Key Laboratory of Luminescence and Optical Information, Ministry of Education, School of Science, Beijing Jiaotong University, Beijing 100044, People's Republic of China.

Strain engineering has become an efficient way to tune the optical and electronic behaviors of metal halide perovskites as a result of their unique structure-dependent optoelectronic characteristics. In this work, we show that the band gap can be reduced and, meanwhile, the carrier lifetime is increased by simply stretching the MAPbICl perovskite thin films. The narrowed band gap and prolonged carrier lifetime are beneficial for the photovoltaic actions, indicating that mechanical stretching can be a simple and efficient way to achieve photovoltaic property optimization of stretchable perovskite-based devices. Furthermore, Raman spectra show that the Pb-I bond length is shortened with mechanical stretching, which increases the valence band maximum (VBM) through orbital coupling, leading to a narrower band gap. Consequently, the trap states near VBM can be radiative as the trap energy levels become closer to the VBM, resulting in a prolonged carrier lifetime. This work brings huge opportunities to control the optoelectronic properties of metal halide perovskites through mechanical stress toward optoelectronic applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c01992DOI Listing
August 2021

Influence of Life Meaning on Subjective Well-Being of Older People: Serial Multiple Mediation of Exercise Identification and Amount of Exercise.

Front Public Health 2021 8;9:515484. Epub 2021 Jul 8.

School of Psychology, Beijing Sport University, Beijing, China.

This study aimed to explore the relationship between life meaning and subjective well-being among older people and the mediating role of subjective exercise identification and objective amount of exercise. A total of 352 older respondents completed four questionnaires: the Chinese life meaning scale, the University of Newfoundland Happiness Scale, the physical activity habits questionnaire, and the exercise identification questionnaire designed for this study. Gender differences existed in the respondents' perception of life meaning, and men had a better perception of life meaning ( = 2.28, = 0.63, < 0.05). There were age differences in the subjective well-being of older people ( = 5.69, η = 0.03, < 0.01); the subjective well-being of older individuals steadily declines with rising age. Life meaning not only directly affected the subjective well-being of the respondents but also indirectly influenced their subjective well-being through the following three pathways: life meaning → exercise identification → subjective well-being (mediating effect of 22%); life meaning → amount of exercise → subjective well-being (mediating effect of 22%); and life meaning → exercise identification → amount of exercise → subjective well-being (serial multiple mediation effect of 27%). The more positive the life meaning perception of older people is, the higher their experience of subjective well-being. For older people to sense the meaning of life, we recommend that they realize the importance of physical activities and take the initiative to engage in physical activities to obtain higher subjective well-being.
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http://dx.doi.org/10.3389/fpubh.2021.515484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295607PMC
July 2021

Unveiling the Metabolic Profile of First-Episode Drug-Naïve Schizophrenia Patients: Baseline Characteristics of a Longitudinal Study Among Han Chinese.

Front Psychiatry 2021 9;12:702720. Epub 2021 Jul 9.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, China.

Metabolic and other medical conditions are frequently comorbid with schizophrenia. As they might be the side-effects of antipsychotic treatment, studying first-episode drug-naïve schizophrenia (FDSZ) provides a unique opportunity to investigate a direct pathogenic link between metabolic changes and schizophrenia. Here, we presented the methods and baseline unique metabolic profile of FDSZ patients without medical comorbidities unveiling subthreshold indices of metabolic disturbances. Drug-naïve individuals diagnosed with schizophrenia but without any previous medical conditions were invited to participate in the study. Participants were submitted to structured psychiatric and cognitive assessments, laboratory and neuroimaging tests. Subjects will be followed after antipsychotic treatment at 6, 24 and 48 weeks. During an 8-month-period, out of 103 patients presenting with first episode psychosis, 67 subjects (43.3% men, 56.7% women) were enrolled in the study. They had a mean ± SD age of 32.1 ± 8.7 years, with a mean BMI of 21.1 kg/m and 11.3 ± 3.6 years of schooling. Less than 1/3 reported a family history of mental illness. Upon laboratory assessment, 10.4%, 7.5%, and 11.9% of patients were identified with hyperhomocysteinemia, hypertriglyceridemia and hyperprolactinemia, respectively, with percentages of women relatively higher than men except for hypertriglyceridemia. First episode schizophrenia patients, especially women, present subclinical metabolic abnormalities, independent of antipsychotic treatment.
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http://dx.doi.org/10.3389/fpsyt.2021.702720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298856PMC
July 2021

Global Leadership Initiative on Malnutrition criteria as a nutrition assessment tool for patients with cancer.

Nutrition 2021 Jun 29;91-92:111379. Epub 2021 Jun 29.

Department of Gastrointestinal Surgery, Department of Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing China; Department of Oncology, Capital Medical University, Beijing, China; Beijing International Science and Technology Cooperation Base for Cancer Metabolism and Nutrition, Beijing, China. Electronic address:

Objectives: Since the launch of Global Leadership Initiative on Malnutrition (GLIM), there has been an urgent need to validate the new criteria, especially in patients with cancer. The aim of this study was to evaluate and validate the use of the GLIM criteria in patients with cancer.

Method: This multicenter cohort study compared the GLIM with the scored Patient-Generated Subjective Global Assessment (sPG-SGA). The 1-y survival rate, multivariate Cox regression analysis, κ-value, sensitivity, specificity, receiver operating characteristic (ROC) curve, and time-dependent ROC analysis were applied to identify the performance of the GLIM.

Results: Among the 3777 patients in the study, 50.9% versus 49.1% or 36.3% versus 63.7% of the patients were defined as well-nourished and malnourished by GLIM or sPG-SGA, respectively. GLIM presented moderate consistency (κ = 0.54, P < 0.001), fair sensitivity and specificity (70.5 and 88.3%) compared with sPG-SGA. There was no difference in the 1-y survival rate in malnourished patients (76.9 versus 76.4%, P = 0.711), but it was significantly different in well-nourished patients (85.8 versus 90.3%, P < 0.001) between GLIM and sPG-SGA. The above difference was eliminated after omitted nutritional risk screening (NRS)-2002 screening before GLIM (88.1 versus 90.3%, P = 0.078). Omitting NRS-2002 screening before GLIM did not change the 1-y survival rate in well-nourished or malnourished patients by GLIM with NRS-2002 screening (76.9 versus 78.9%, P = 0.099; 85.8% versus 88.1%, P = 0.092) although it significantly raised the rate of malnutrition to 72.5%. The combination of "weight loss and cancer" showed better performance than other combinations.

Conclusions: GLIM could be a convenient alternative to sPG-SGA in nutrition assessment for patients with cancer. The combination of "weight loss and cancer" was better than other combinations. Considering the higher risk for malnutrition in patients with cancer, NRS-2002 screening may not be needed before GLIM.
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http://dx.doi.org/10.1016/j.nut.2021.111379DOI Listing
June 2021

(-)-Syringaresinol suppressed LPS-induced microglia activation via downregulation of NF-κB p65 signaling and interaction with ERβ.

Int Immunopharmacol 2021 Jul 22;99:107986. Epub 2021 Jul 22.

Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair, Institute of Acute Abdominal Diseases, Tianjin Nankai Hospital, Tianjin 300100, China. Electronic address:

Albiziae Cortex (AC) is a well-known traditional Chinese medicine with sedative-hypnotic effects and neuroprotective ability. However, the bioactive components of AC responsible for the neuro-protective actitivity remain unknown. Here, we investigated the anti-neuroinflammatory effects of (-)-syringaresinol (SYR) extracted from AC in microglia cells and wild-type mice. As a result, (-)-SYR significantly reduced lipopolysaccharide (LPS)-induced production of interleukin - 6 (IL-6), tumor necrosis factor α (TNF-α), interleukin -1 beta (IL-1β), cycloxygenase-2 (COX-2), and nitric oxide (NO) in BV2 microglia cells. (-)-SYR also significantly reduced M1 marker CD40 expression and increased M2 marker CD206 expression. Moreover, we found that (-)-SYR inhibited LPS-induced NF-κB activation by suppressing the translocation of NF-κB p65 into the nucleus in a concentration-dependent manner. Meanwhile, estrogen receptor β (ERβ) was found to be implied in the anti-inflammatory activity of (-)-SYR in BV2 microglia. In vivo experiments revealed that administration of (-)-SYR in mice significantly reduced microglia/astrocytes activation and mRNA levels of proinflammatory mediators. Taken together, our data indicated that (-)-SYR exerted the anti-neuroinflammatory effects by inhibiting NF-κB activation and modulation of microglia polarization, and via interaction with ERβ. The anti-neuroinflammatory activity of (-)-SYR may provide a new therapeutic avenue for the treatment of brain diseases associated with inflammation.
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http://dx.doi.org/10.1016/j.intimp.2021.107986DOI Listing
July 2021

Prenatal perception of breastfeeding recommendation predicts early breastfeeding outcomes of participants in the Supplemental Nutrition Program for Women, Infants, and Children (WIC).

Am J Clin Nutr 2021 Jul 24. Epub 2021 Jul 24.

Department of Sociology, California State University-Dominguez Hills, Carson, CA.

Background: Prenatal psychosocial factors predict breastfeeding practices but are not assessed in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC).

Objective: This study examined how prenatal perceptions of WIC's breastfeeding recommendations were associated with early breastfeeding outcomes.

Methods: This study used longitudinal data from a national sample of 2,053 pregnant participants in the WIC Infant and Toddler Feeding Practices Study-2 (WIC ITFPS-2) in 2013, the only national data assessing prenatal perceptions of WIC's breastfeeding recommendations. Early breastfeeding outcomes included breastfeeding initiation, breastmilk first fed after birth, breastfeeding in the first hour, breastmilk first fed after leaving the hospital, and breastfeeding status at the first and third month. The primary predictor was the participant's prenatal perception of whether WIC recommended breastfeeding only or not. Log binomial regression was used with adjustment for socio-demographics, previous breastfeeding, WIC participation, breastfeeding support, and infant feeding intentions (IFI).

Results: Without controlling for IFI, the perception of WIC recommending breastfeeding only predicted breastfeeding outcomes positively. The risk ratio (RR) associated with prenatal perceptions varied from 1.14 (95% Confidence Interval [CI]: 1.03, 1.25; P = 0.008) for breastfeeding in the first hour, 1.27 (95% CI: 1.12, 1.43; P < 0.001) for breastmilk first fed after leaving hospital, to 1.66 (95% CI: 1.35, 2.04; P < 0.001) for 3-month breastfeeding only. After controlling for IFI, the RRs were 1.13 (95% CI: 1.02, 1.24; P = 0.017) for breastfeeding in the first hour, 1.20 (95% CI: 1.06, 1.35; P = 0.004) for breastmilk first fed after leaving hospital, and 1.49 (95% CI: 1.21, 1.84; P < 0.001) for 3-month breastfeeding only, suggesting that prenatal perception was independently associated with breastfeeding outcomes.

Conclusions: Prenatal perception of WIC's breastfeeding recommendations can be a new psychosocial predictor of breastfeeding and a possible target for future intervention.
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http://dx.doi.org/10.1093/ajcn/nqab268DOI Listing
July 2021

Inhibition of lung tumorigenesis by a small molecule CA170 targeting the immune checkpoint protein VISTA.

Commun Biol 2021 Jul 23;4(1):906. Epub 2021 Jul 23.

Center for Disease Prevention Research, Medical College of Wisconsin, Milwaukee, WI, USA.

Expressed on cells of the myeloid and lymphoid lineages, V-domain Ig Suppressor of T cell Activation (VISTA) is an emerging target for cancer immunotherapy. Blocking VISTA activates both innate and adaptive immunity to eradicate tumors in mice. Using a tripeptide small molecule antagonist of VISTA CA170, we found that it exhibited potent anticancer efficacy on carcinogen-induced mouse lung tumorigenesis. Remarkably, lung tumor development was almost completely suppressed when CA170 was combined with an MHCII-directed KRAS peptide vaccine. Flow cytometry and single-cell RNA sequencing (scRNA-seq) revealed that CA170 increased CD8 T cell infiltration and enhanced their effector functions by decreasing the tumor infiltration of myeloid-derived suppressor cells (MDSCs) and Regulatory T (Treg) cells, while the Kras vaccine primarily induced expansion of CD4 effector T cells. VISTA antagonism by CA170 revealed strong efficacy against lung tumorigenesis with broad immunoregulatory functions that influence effector, memory and regulatory T cells, and drives an adaptive T cell tumor-specific immune response that enhances the efficacy of the KRAS vaccine.
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http://dx.doi.org/10.1038/s42003-021-02381-xDOI Listing
July 2021

Programmed cell death-ligand 1 expression predicts poor treatment response and prognostic value in esophageal squamous cell carcinoma patients without esophagectomy.

Aging (Albany NY) 2021 Jul 22;13(14):18827-18838. Epub 2021 Jul 22.

Department of Radiation Oncology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 26400, Shandong, China.

Research on association between programmed cell death ligand 1 (PD-L1) expression in cancer cells and prognosis of esophageal squamous cell carcinoma (ESCC) has been controversial and has focused on patients with surgical resection. We aimed to investigate impact of PD-L1 on treatment response and prognostic value in ESCC and analyze which subset of patients may benefit from immunotherapy. The PD-L1 expression was evaluated by immunohistochemical analysis in all patients. Stratification analysis was performed according to whether surgery was performed. There were no significant correlations between PD-L1 expression with 3-year overall survival (OS) and progression-free survival (PFS) in 81 ESCC patients. Then stratification analysis was performed. Among these 44 patients without surgery, disease control rate (DCR) in negative PD-L1 expression group (78%) was significantly better than those (42%) in positive PD-L1 expression group ( = 0.032). There were no significant correlations between PD-L1 expression with 3-year OS and PFS in 37 ESCC patients receiving surgery. However, in 44 ESCC patients without surgery, the Kaplan-Meier method showed that 3-year OS and PFS in negative PD-L1 expression group were significantly better than those in positive PD-L1 expression group. In Cox univariate and multivariate model, PD-L1 was an independent prognosticator for inferior OS ( = 0.011; = 0.017). Our research revealed prognostic role of PD-L1 expression in cancer cells may be variable in different treatment methods. Consequently, PD-L1 may serve as an independent prognostic factor and provide a theoretical basis for combining conventional therapy with immunotherapy targeting PD-L1 to achieve better treatment outcome in ESCC patients without esophagectomy.
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http://dx.doi.org/10.18632/aging.203326DOI Listing
July 2021

Efficacy and safety of induction chemotherapy with daunorubicin or idarubicin in the treatment of an adult with acute lymphoblastic leukemia.

Tumori 2021 Jul 23:3008916211032724. Epub 2021 Jul 23.

Department of Hematology and Rheumatology, The Second Affiliated Hospital of Mudanjiang University, Mudanjiang, China.

Background: Anthracyclines are dispensable components of chemotherapy of patients with acute lymphoblastic leukemia (ALL).

Objective: To analyze the efficacy and safety of induction with idarubicin (IDA) or liposoma daunorubicin (L-DNR) in treatment of adults with high-risk ALL (HR-ALL) (presence of mixed lineage leukemia gene [] rearrangements, t[1;19], or prednisone poor response).

Methods: Among 58 enrolled patients, 29 cases were defined as the IDA group and the other 29 patients were put into the L-DNR group. Both overall survival (OS) and progression-free survival (PFS) were estimated and overall response rate (ORR) was compared between the groups.

Results: The L-DNR group's OS and PFS were insignificantly higher than in the IDA group (=0.261 and =0.247). Although not significantly different, the ORR of adults with HR-ALL receiving L-DNR regimens was also higher than in the IDA group (=0.085). Comprehensive cytogenetic analysis revealed that patients harboring rearrangement, , and mutation had poorer prognosis than others. All 58 patients experienced hematologic response in this study; however, the length of hematologic response in the IDA group was significantly longer than in the L-DNR group (=0.005). The incidence of bleeding and infection was without significant difference between the groups (both >0.05).

Conclusion: L-DNR proved to be an effective drug within a multiagent approach, which shows a favorable overall profile, as well as similar adverse events when compared with IDA in HR-ALL. Patients with are much more sensitive to L-DNR than IDA. Despite some progress made, outcomes in rearrangement or mutation carriers remain unsatisfactory, and intensive treatment will be critical.
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http://dx.doi.org/10.1177/03008916211032724DOI Listing
July 2021

The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis.

Biochem Pharmacol 2021 Jul 19;192:114691. Epub 2021 Jul 19.

Clinical Pharmacy Center, Department of Pharmacy, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, China; Key Laboratory of Endocrine Gland Diseases of Zhejiang Province, Hangzhou 310014, Zhejiang, China. Electronic address:

Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease caused by an interaction of genetics, immune responses, and environmental factors. However, the precise pathogenesis of UC remains poorly understood. The aim of the present study was to explore the potential target genes implicated in UC and to elucidate its underlying pathogenic mechanism. Firstly, three UC-associated microarray datasets (GSE75214, GSE87466, and GSE92517) were obtained to screen the differentially expressed genes (DEGs). As a result, alcohol dehydrogenase 1C (ADH1C) was the most significantly downregulated DEG in UC. We confirmed that ADH1C was downregulated in colonic tissue taken from patients with UC and colitis mice. Moreover, ADH1C expression was also decreased in colonic cell lines (NCM460 and HT29) treated with mixed proinflammatory cytokines (TNF-α, IFN-γ, IL-1β). Interestingly, we found that overexpression of ADH1C could distinctly decrease the production of IL-6 and IL-8. To elucidate the molecular mechanism of ADH1C in UC, gene set enrichment analysis (GSEA) was performed and demonstrated that immune-related pathways were mainly enriched in the low ADH1C group. Further studies displayed that STAT1/NF-κB pathway was activated in colitis mice and inflammatory cell model. Importantly, overexpression of ADH1C could suppress the phosphorylation of STAT1 and IκB. Therefore, ADH1C might regulate inflammatory responses through STAT1/NF-κB pathway. In conclusion, ADH1C was downregulated during inflammation, and its increased expression could inhibit the activation of STAT1/NF-κB pathway, thereby alleviating the secretion of IL-6 and IL-8. These findings indicate that ADH1C may be a potential therapeutic target for UC therapy.
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http://dx.doi.org/10.1016/j.bcp.2021.114691DOI Listing
July 2021

Coupling relationship between glucose and oxygen metabolisms to differentiate preclinical Alzheimer's disease and normal individuals.

Hum Brain Mapp 2021 Jul 22. Epub 2021 Jul 22.

Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Joint International Research Laboratory of Specialty Fiber Optics and Advanced Communication, School of Communication and Information Engineering, Shanghai University, Shanghai, China.

The discovery of preclinical Alzheimer's disease (preAD) provides a wide time window for the early intervention of AD. The coupling relationships between glucose and oxygen metabolisms from hybrid PET/MRI can provide complementary information on the brain's physiological state for preAD. In this study, we purpose to explore the change of coupling relationship among 27 normal controls (NCs), 20 preADs, and 15 cognitive impairments (CIs). For each subject, we calculated the Spearman partial correlation between the fractional amplitude of low-frequency fluctuations (fALFF) and the regional homogeneity (ReHo) from functional image (fMRI), and the standard uptake value ratio (SUVR) from [18F] fluorodeoxyglucose positron emission tomography ( F-FDG PET), in the whole-brain and default mode network (DMN) as a novel potential biomarker. The diagnostic performance of this biomarker was evaluated by the receiver operating characteristic analysis. Significant Spearman correlations between the FDG SUVR and the fALFF/ReHo were found in 98% of subjects. For the DMN-based biomarker, there was a significant decreasing trend for the preAD and CI groups compared to the NC group, whereas no significant difference in preAD based on whole-brain. The correlation ρ value for the FDG SUVR/ReHo showed the highest area under curve of the preAD classification (0.787). The results imply the coupling relationship changed during the preAD stage in the DMN area.
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http://dx.doi.org/10.1002/hbm.25599DOI Listing
July 2021

Tumor size and perineural invasion predict outcome of gastric high grade neuroendocrine neoplasms.

Endocr Connect 2021 Jul 1. Epub 2021 Jul 1.

Y Sun, Department of General Surgery, Zhongshan Hospital Fudan University, Shanghai, China.

A new subcategory, grade 3 neuroendocrine tumors, is incorporated into the grading system of pancreatic neuroendocrine neoplasms in the 2017 WHO classification in order to differentiate grade 3 neuroendocrine tumors from neuroendocrine carcinomas. The 2019 WHO classification extends the concept of grade 3 neuroendocrine tumors to gastrointestinal high grade neuroendocrine neoplasms. However, there is still limited study focusing on the gastric grade 3 neuroendocrine tumors and gastric neuroendocrine carcinomas. We retrospectively enrolled 151 gastric high grade neuroendocrine neoplasms patients, who underwent radical resection from January 2007 to December 2015. Clinicopathologic and prognostic features were studied. The Surveillance, Epidemiology, and End Results database was used to verify the prognostic determinants found in Zhongshan cohort. Neuroendocrine carcinomas showed higher Ki67 index and higher mitotic count than grade 3 neuroendocrine tumors. We identified 109 (72.2%) patients of neuroendocrine carcinomas, 12 (7.9%) patients of grade 3 neuroendocrine tumors and 30 (19.9%) patients of mixed neuroendocrine non-neuroendocrine neoplasms. Although neuroendocrine carcinomas demonstrated higher Ki67 index (p=0.004) and mitoses (p=0.001) than grade 3 neuroendocrine tumors, their prognosis after radical resection did not demonstrate significant differences (p=0.709). Tumor size, perineural invasion and TNM stage were independent prognostic factors of gastric high grade neuroendocrine neoplasms.
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http://dx.doi.org/10.1530/EC-21-0017DOI Listing
July 2021
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