Publications by authors named "Qi Yao"

205 Publications

Tumor-Derived Autophagosomes (DRibbles) Activate Human B Cells to Induce Efficient Antigen-Specific Human Memory T-Cell Responses.

Front Immunol 2021 26;12:675822. Epub 2021 May 26.

Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing, China.

We have reported that tumor-derived autophagosomes (DRibbles) were efficient carriers of tumor antigens and DRibbles antigens could be present by DRibbles-activated B cells to stimulate effect and naïve T cells in mice. However, the effect of DRibbles on human B cells remains unclear. Herein, we found that DRibbles can also efficiently induce proliferation and activation of human B cells and lead to the production of chemokines, cytokines and hematopoietic growth factors. We further demonstrated human B cells can effectively phagocytose DRibbles directly and cross-present DRibbles antigens to stimulate antigen-specific memory T cells. Furthermore, we found that membrane-bound high-mobility group B1 (HMGB1) on DRibbles was crucial for inducing human B cells activation. Therefore, these findings provide further evidence to promote the clinical application of B-DRibbles vaccines.
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http://dx.doi.org/10.3389/fimmu.2021.675822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187759PMC
May 2021

MiR-223 or miR-126 predicts resistance to dual antiplatelet therapy in patients with ST-elevation myocardial infarction.

J Int Med Res 2021 Jun;49(6):3000605211016209

Cardiovascular Center of Ningbo First Hospital, Ningbo, China.

Objective: To explore the role of miR-223 and miR-126 in predicting treatment responses to dual antiplatelet therapy (DAPT) in patients with ST-elevation myocardial infarction (STEMI).

Methods: Plasma miR-223 and miR-126 levels were measured before treatment. Treatment responses and 2-year survival were determined. experiments were performed to explore the mechanism of action.

Results: Patients with resistance to DAPT had a lower level of miR-223 and miR-126. Cardiac-event-free survival was shorter in patients with lower miR-223 or miR-126 levels. MiR-223 and miR-126 independently predicted DAPT resistance. Modulating miR-223 or miR-126 in platelets significantly changed the response to clopidogrel by regulating platelet aggregation.

Conclusion: MiR-223 and miR-126 play a role in DAPT resistance and may provide potential biomarkers in patients with STEMI.
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http://dx.doi.org/10.1177/03000605211016209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191085PMC
June 2021

Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans.

BMC Biol 2021 May 20;19(1):108. Epub 2021 May 20.

Institute of Genomics, School of Medicine, Huaqiao University, 668 Jimei Road, Xiamen, 361021, China.

Background: The majority of the human genome is transcribed in the form of long non-coding (lnc) RNAs. While these transcripts have attracted considerable interest, their molecular mechanisms of function and biological significance remain controversial. One of the main reasons behind this lies in the significant challenges posed by lncRNAs requiring the development of novel methods and concepts to unravel their functionality. Existing methods often lack cross-validation and independent confirmation by different methodologies and therefore leave significant ambiguity as to the authenticity of the outcomes. Nonetheless, despite all the caveats, it appears that lncRNAs may function, at least in part, by regulating other genes via chromatin interactions. Therefore, the function of a lncRNA could be inferred from the function of genes it regulates. In this work, we present a genome-wide functional annotation strategy for lncRNAs based on identification of their regulatory networks via the integration of three distinct types of approaches: co-expression analysis, mapping of lncRNA-chromatin interactions, and assaying molecular effects of lncRNA knockdowns obtained using an inducible and highly specific CRISPR/Cas13 system.

Results: We applied the strategy to annotate 407 very long intergenic non-coding (vlinc) RNAs belonging to a novel widespread subclass of lncRNAs. We show that vlincRNAs indeed appear to regulate multiple genes encoding proteins predominantly involved in RNA- and development-related functions, cell cycle, and cellular adhesion via a mechanism involving proximity between vlincRNAs and their targets in the nucleus. A typical vlincRNAs can be both a positive and negative regulator and regulate multiple genes both in trans and cis. Finally, we show vlincRNAs and their regulatory networks potentially represent novel components of DNA damage response and are functionally important for the ability of cancer cells to survive genotoxic stress.

Conclusions: This study provides strong evidence for the regulatory role of the vlincRNA class of lncRNAs and a potentially important role played by these transcripts in the hidden layer of RNA-based regulation in complex biological systems.
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http://dx.doi.org/10.1186/s12915-021-01044-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139166PMC
May 2021

The Traditional Uses, Phytochemistry, Pharmacology, Toxicology, and Clinical Uses of (Franch.) T.N.Ho and S.W.Liu, an Ethnomedicine in Southwest China.

Front Pharmacol 2021 26;12:658628. Epub 2021 Apr 26.

The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China.

(Franch.) T.N.Ho and S.W.Liu (MR) belongs to Gentianales and it is often called Hong-hua-long-dan in Chinese. Traditionally, it has been used to cure acute icteric hepatitis, sore throat, dysentery, acute gastritis, carbuncle, and furuncle based on traditional Chinese medicine (TCM) concepts. This review manages to provide a critical and comprehensive analysis on the traditional uses, phytochemistry, pharmacology, toxicology, and clinical uses of MR and to evaluate the therapeutic potential of this plant. Relevant data mainly literatures on MR were selected from available database. All the papers reviewed provided evidence that the source herbs were reliably identified. The heat-clearing and removing the phlegm, and purging fire and removing toxicity of MR contribute to its dispelling jaundice, and clearing lung heat and cough. The compounds isolated from this plant include iridoids and secoiridoids, phenolic acids, ketones, triterpenoids, flavonoids, benzophenone glycosides, and others. Mangiferin (MAF) is a characteristic substance from this plant. The pharmacological studies show that some extracts and compounds from MR exhibit anti-inflammatory, antinociceptive, antibacterial, hepatoprotective, cardioprotective, and other effects which are associated with the traditional uses of this plant. The toxicological studies suggest that MAF is less toxic in mice and dogs. Nowadays, Chinese patent drugs such as Feilike Jiaonang and Kangfuling Jiaonang containing MR have been used to cure cough, asthma, chronic bronchitis, dysmenorrhea, and appendagitis. Although the current studies provide related research information of MR, it is still necessary to systemically evaluate the chemistry, pharmacology, toxicity, and safety of the extracts or compounds from this plant before clinical trials in the future. In addition, except for lung infection-related diseases, analgesia, anti-tumor, and hypertriglycemia may be new and prior therapeutic scopes of this ethnomedicine in the future.
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http://dx.doi.org/10.3389/fphar.2021.658628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107381PMC
April 2021

Relationship between the of Smart Meta-Superconductor Bi(Pb)SrCaCuO and Inhomogeneous Phase Content.

Nanomaterials (Basel) 2021 Apr 21;11(5). Epub 2021 Apr 21.

Smart Materials Laboratory, Department of Applied Physics, Northwestern Polytechnical University, Xi'an 710129, China.

A smart meta-superconductor Bi(Pb)SrCaCuO (B(P)SCCO) may increase the critical transition temperature () of B(P)SCCO by electroluminescence (EL) energy injection of inhomogeneous phases. However, the increase amplitude Δ (ΔTC=TC-TC,pure) of is relatively small. In this study, a smart meta-superconductor B(P)SCCO with different matrix sizes was designed. Three kinds of raw materials with different particle sizes were used, and different series of YO:Sm, YO, YO:Eu, and YO:Eu+Ag-doped samples and pure B(P)SCCO were prepared. Results indicated that the of the YO or YO:Sm non-luminescent dopant doping sample is lower than that of pure B(P)SCCO. However, the of the YO:Eu+Ag or YO:Eu luminescent inhomogeneous phase doping sample is higher than that of pure B(P)SCCO. With the decrease of the raw material particle size from 30 to 5 μm, the particle size of the B(P)SCCO superconducting matrix in the prepared samples decreases, and the doping content of the YO:Eu+Ag or YO:Eu increases from 0.2% to 0.4%. Meanwhile, the increase of the inhomogeneous phase content enhances the Δ. When the particle size of raw material is 5 μm, the doping concentration of the luminescent inhomogeneous phase can be increased to 0.4%. At this time, the zero-resistance temperature and onset transition temperature of the YO:Eu+Ag doped sample are 4 and 6.3 K higher than those of pure B(P)SCCO, respectively.
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http://dx.doi.org/10.3390/nano11051061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143111PMC
April 2021

Endophytic fungus Mucor circinelloides DF20 promote tanshinone biosynthesis and accumulation in Salvia miltiorrhiza root.

Plant Sci 2021 Jun 30;307:110898. Epub 2021 Mar 30.

Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China. Electronic address:

As a traditional Chinese medicine, Salvia miltiorrhiza rhizome is mainly used to treat cardiovascular diseases. Symbiosis of endophytic fungi with their host plants, is an effectively regulatory means to promote the growth and secondary metabolism of medicinal plants. Here, an endophytic fungus Mucor circinelloides DF20 was co-cultivated with the sterile seedlings of S. miltiorrhiza, to clarify the promoting mechanism on tanshinone biosynthesis and accumulation in S. miltiorrhiza root. The assay of promoting-growth activities in vitro showed that DF20 have the ability to produce IAA and siderophores. DF20 could significantly promote the biosynthesis and accumulation of tanshinones in the root of S. miltiorrhiza, especially the content of tanshinone ⅡA, reaching 4.630 ± 0.342 mg/g after 56 days of DF20 treatment, which is 22-fold of the control group. The result also showed that the hyphae of M. circunelloides DF20 mainly colonized in the root tissue interspace of S. miltiorrhiza, and a small amount of hyphae were located inside the cells. The results of florescent real-time quantitative RT-PCR showed that DF20 colonization significantly increase the expression level of some key enzyme genes (DXS, DXR, HMGR, GGPPS) in tanshinone biosynthesis pathway, but the regulatory effect mainly occurred in the early stage of co-culture, while the expression level decreased in different degrees in the later stage. In conclusion, the endophytic fungus M. circunelloides DF20 can form an interaction relationship with its host, then to promote the biosynthesis and accumulation of tanshinones in root by upregulating the key enzyme genes expression levels of the biosynthesis pathway.
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http://dx.doi.org/10.1016/j.plantsci.2021.110898DOI Listing
June 2021

Estimating the impact of ground ozone concentrations on crop yields across China from 2014 to 2018: A multi-model comparison.

Environ Pollut 2021 Aug 7;283:117099. Epub 2021 Apr 7.

State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Global and Earth System Sciences, Beijing Normal University, 19 Xinjiekou Street, Haidian, Beijing, 100875, China. Electronic address:

Ground level ozone exerts a strong impact on crop yields, yet how to properly quantify ozone induced yield losses in China remains challenging. To this end, we employed a series of O-crop models to estimate ozone induced yield losses in China from 2014 to 2018. The outputs from all models suggested that the total Relative Yield Losses (RYL) of wheat in China from 2014 to 2018 was 18.4%-49.3% and the total RYL of rice was 6.2%-52.9%. Consequently, the total Crop Production Losses (CPL) of wheat and rice could reach 63.9-130.4 and 28.3-35.4 million tons, and the corresponding Total Economic Losses (TEL) could reach 20.5-44.7 and 11.0-15.3 billion dollars, stressing the great importance and urgency of national ozone management. Meanwhile, the estimation outputs highlighted the large variations between different regional O-crop models when applying to large scales. Instead of applying one unified O-crop models to all regions across China, we also explored the strategy of employing specific O-crop models in corresponding (and neighboring) regions to estimate ozone induced yield loss in China. The comparison of two strategies suggested that the mean value from multiple models may still present an inconsistent over/underestimation trend for different crops. Therefore, it is a preferable strategy to employ corresponding O-crop models in different regions for estimating the national crop losses caused by ozone pollution. However, the severe lack of regional O-crop models in most regions across China makes a robust estimation of national yield losses highly challenging. Given the large variations between O-crop interactions across regions, a systematic framework with massive regional O-crop models should be properly designed and implemented.
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http://dx.doi.org/10.1016/j.envpol.2021.117099DOI Listing
August 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

J Int Med Res 2021 Apr;49(4):300060521998471

Department of Geriatrics, Ningbo First Hospital, Ningbo, China.

Objective: Parkinson's disease (PD) is a degenerative disorder characterized by steady motor function loss. PD pathogenesis remains inconclusive, but aberrant immune responses might play important roles. We hypothesized that imbalance between T helper (Th) 1 and regulatory T (Treg) cells was essential in experimental PD.

Methods: Th1 and Treg cells from the blood of patients with PD and healthy volunteer blood were measured by flow cytometry. Experimental PD was induced in mice by peritoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Experimental PD severity was measured by open field test behavior assessments (distance moved, rearing, and grooming). Mice were administered neutralizing anti-tumor necrosis factor (TNF) α to inhibit Th1 effects. Treg cells were depleted by anti-CD25 neutralizing antibodies or isolated and transferred to experimental PD mice.

Results: Patients with PD had fewer Treg and more Th1 cells than healthy volunteers. Experimental PD mice exhibited fewer Treg and more Th1 cells. Treg cell depletion exacerbated experimental PD, whereas TNFα neutralization attenuated PD in mice. Treg transfer to experimental PD mice reduced PD severity. Mechanistically, anti-TNFα antibody administration and Treg transfer increased Treg and reduced Th1 cell abundance in the brain.

Conclusion: Th1 and Treg cell imbalance plays an essential role in mouse experimental PD pathogenesis.
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http://dx.doi.org/10.1177/0300060521998471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053775PMC
April 2021

J.R.Forst. and G.Forst.: A Review of Its Traditional Uses, Chemistry, Pharmacology, Toxicology and Applications.

Front Pharmacol 2020 8;11:608199. Epub 2021 Feb 8.

The First Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, China.

J.R.Forst. and G.Forst (DRF; Convolvulaceae, called Matijin in Chinese), has been traditionally used to treat jaundice, bacillary dysentery, urinary tract infection, edema, contusions, and strains and sprains based on traditional Chinese medicine (TCM) concepts. This paper intends to provide a comprehensive and critical analysis of research on DRF focusing on a relationship between traditional uses and pharmacological effects, evaluating the therapeutic potential of this plant. Relevant data on DRF were retrieved from available databases. The heat-clearing and detoxifying, and removing the phlegm and turbid urine effects of DRF are linked to its anti-hepatitis B virus (HBV), anti-inflammatory, and hepatoprotective activities. Especially, the hepatoprotective effects of DRF are mainly based on anti-HBV activities of phenylalanine dipeptides Matijin-Su (MTS) and its derivatives derived from this plant. Further, a phase I anti-HBV clinical trial of a candidate compound named bentysrepinine (Y101, Chinese name Tifentai) has been completed. Also, anti-tumor, analgesic, and antibacterial properties have been reported in the extracts and compounds from DRF. Although pharmacy, pharmacodynamics, toxicology, and pharmacokinetics of bentysrepinine have been systemically reported, no studies have reported chemistry, safety, pharmacology of other compounds or extracts systemically. Phenylalanine dipeptide compounds are main components and MTS is a characteristic substance of DRF. The main pharmacological effect of DRF is anti-HBV activity, which is coherent with the traditional use of this plant in China. Except bentysrepinine, few studies have been conducted on toxicities of the extracts or compounds from DRF. Thus, it is still necessary to evaluate safety, chemistry, pharmacology of the extracts or compounds from DRF regarding the link between traditional uses and modern applications before the future clinical trials. Bacterial sepsis, cholecystitis and tumors may be prior therapeutic targets of this plant in the future.
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http://dx.doi.org/10.3389/fphar.2020.608199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908059PMC
February 2021

Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells.

J Mater Sci Mater Med 2021 Feb 27;32(2):20. Epub 2021 Feb 27.

State Key Laboratory of Ophthalmology, Optometry and Visual Science, Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China.

Arguments regarding the biocompatibility of graphene-based materials (GBMs) have never ceased. Particularly, the genotoxicity (e.g., DNA damage) of GBMs has been considered the greatest risk to healthy cells. Detailed genotoxicity studies of GBMs are necessary and essential. Herein, we present our recent studies on the genotoxicity of most widely used GBMs such as graphene oxide (GO) and the chemically reduced graphene oxide (RGO) toward human retinal pigment epithelium (RPE) cells. The genotoxicity of GO and RGOs against ARPE-19 (a typical RPE cell line) cells was investigated using the alkaline comet assay, the expression level of phosphorylated p53 determined via Western blots, and the release level of reactive oxygen species (ROS). Our results suggested that both GO and RGOs induced ROS-dependent DNA damage. However, the DNA damage was enhanced following the reduction of the saturated C-O bonds in GO, suggesting that surface oxygen-containing groups played essential roles in the reduced genotoxicity of graphene and had the potential possibility to reduce the toxicity of GBMs via chemical modification.
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http://dx.doi.org/10.1007/s10856-021-06491-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914238PMC
February 2021

SMAR1 attenuates the stemness of osteosarcoma cells via through suppressing ABCG2 transcriptional activity.

Environ Toxicol 2021 Jun 5;36(6):1090-1098. Epub 2021 Feb 5.

Department of Geriatric Medicine, Ningbo First Hospital, Ningbo, China.

The promoting roles of the transcriptional regulator SMAR1 have been revealed in several tumors, such as colorectal and breast cancer, however, its roles in osteosarcoma (OS) progression are still confusing. Here, we find that SMAR1 expression is positively correlated with the overall survival of OS patients and negatively correlated with the expression of stemness markers by analyzing the online datasets. Through analyzing different Gene Expression Omnibus (GEO) datasets, SMAR1 is found to be lowly expressed in OS tissues relative to that in adjacent tissues. Functional experiments indicate that SMAR1 overexpression attenuates the stemness of OS cells, characterized as the decrease of stemness marker expression, sphere-formation ability and ALDH activity. Mechanistically, it is shown that SMAR1 increases the deacetylation level of the drug efflux pump ABCG2 via recruiting HDAC2 to the promoter of the gene coding ABCG2, and thus decreases ABCG2 transcriptional activity. Additionally, overexpression of ABCG2 rescues the inhibition of SMAR1 overexpression on the stemness of OS cells. Moreover, this SMAR1/ABCG2 axis positively regulates the chemotherapeutic sensitivity of OS cells. This work indicates that SMAR1 is a critical suppressor for OS progression through transcriptionally regulating ABCG2 expression.
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http://dx.doi.org/10.1002/tox.23108DOI Listing
June 2021

Significant improvement in thermoelectric performance of SnSe/SnS nano-heterostructures.

Phys Chem Chem Phys 2021 Feb;23(6):3794-3801

Hubei Nuclear Solid Physics Key Laboratory, Department of Physics, Wuhan University, Wuhan 430072, China.

In this work, we study theoretically the electronic and phonon transport properties of heterojunction SnSe/SnS, bilayer SnSe and SnS. The energy filtering effect caused by the nano heterostructure in SnSe/SnS induces an increase in the Seebeck coefficient, causing a large power factor. We calculate the phonon relaxation time and lattice thermal conductivity κL for the three structures; the heterogeneous nanostructure could effectively reduce κL due to the enhanced phonon boundary scattering at interfaces. The average κL notably reduces from around 3.3 (3.2) W m-1 K-1 for bilayer SnSe (SnS) to nearly 2.2 W m-1 K-1 for SnSe/SnS at 300 K. As a result, the average ZT (ZTave in b and c directions) reaches 1.63 with temperature range around 300-800 K, which is improved by 63% (25%) compared with that of bilayer SnSe (SnS). Our theoretical results show that the heterogeneous nanostructure is an innovative approach for improving the Seebeck coefficient and significantly reducing κL, effectively enhancing thermoelectric properties.
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http://dx.doi.org/10.1039/d0cp05548dDOI Listing
February 2021

Vagus Nerve Stimulation Decreases Pancreatitis Severity in Mice.

Front Immunol 2020 14;11:595957. Epub 2021 Jan 14.

Department of Critical Care Medicine, Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Vagus nerve stimulation (VNS) is effective in reducing inflammation in various diseases, such as rheumatoid arthritis, colitis and acute kidney injury. The anti-inflammatory effect of vagus nerve in these diseases necessitates the interactions of neural activation and α7 nicotinic acetylcholine receptors (α7nAChRs) on splenic macrophages. In this study, we aimed to investigate the effect of VNS on severity in experimental acute pancreatitis (AP).

Methods: Two independent AP models were used, which induced in ICR mice with caerulein or pancreatic duct ligation (PDL). Thirty minutes after modeling, the left cervical carotid sheath containing the vagus nerve was electrically stimulated for 2 min. Plasma lipase and amylase activities, TNF-α levels and pancreas histologic damage were evaluated. In caerulein mice, the percentages of α7nAChR macrophage in pancreas and spleen were assessed by flow cytometry. Furthermore, splenectomy and adoptive transfer of VNS-conditioned α7nAChR splenocytes were performed in caerulein mice to evaluate the role of spleen in the protective effect of VNS.

Results: VNS reduced plasma lipase and amylase activities, blunted the concentrations of TNF-α and protected against pancreas histologic damage in two AP models. Survival rates were improved in the PDL model after VNS. In caerulein AP mice, VNS increased the percentages of α7nAChR macrophages in pancreas and spleen. Adoptive transfer of VNS-treated α7nAChR splenocytes provided protection against pancreatitis in recipient mice. However, splenectomy did not abolish the protective effect of VNS.

Conclusions: VNS reduces disease severity and attenuates inflammation in AP mice. This effect is independent of spleen and is probably related to α7nAChR on macrophage.
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http://dx.doi.org/10.3389/fimmu.2020.595957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840568PMC
June 2021

Clinical characteristics and outcomes in coronavirus disease 2019 (COVID-19) patients with and without hypertension: a retrospective study.

Rev Cardiovasc Med 2020 12;21(4):615-625

Department of Cardiology, Renmin Hospital of Wuhan University, 430060, Wuhan, P. R. China.

The novel coronavirus disease (COVID-19) has spread all over the world in a short time. Information about the differences between COVID-19 patients with and without hypertension is limited. To explore the characteristics and outcomes differences between COVID-19 patients with and without hypertension, the medical records and cardiac biomarkers of 414 patients were analyzed. A total of 149 patients had a history of hypertension, while 265 patients did not have hypertension, and the groups were compared based on their clinical characteristics and laboratory findings as well as the hazard risk for composite outcomes, including intensive care unit (ICU) admission, mechanical ventilation, or death. The results are as follows. On admission, 22.1% of patients in hypertension group had elevated high sensitivity troponin I (hs-TNI > 26 pg/mL), which was higher than the proportion in the nonhypertension group (6.4%). Median NT-proBNP levels in patients with hypertension (141.9 pg/mL) were higher than those in patients without hypertension (77.3 pg/mL). Patients in the hypertension group had a higher risk for in-hospital death [HR: 2.57, 95% CI (1.46~4.51)]. However, the impact of hypertension on the prognosis was not significant after adjusting for age and sex. Multivariate Cox hazard regression confirmed that NT-proBNP levels in the highest tertile (upper 75 % of patients with hypertension) was an independent risk factor for in-hospital death in all COVID-19 patients. Taken together, hypertension per se had a modest impact on the prognosis in COVID-19 patients. In COVID-19 patients with and without hypertension, NT-proBNP may be a better predictor of prognosis than hs-TNI.
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http://dx.doi.org/10.31083/j.rcm.2020.04.113DOI Listing
December 2020

Vaccine of RANKL mutant conjugated with KLH effectively stabilizing bone metabolism and preventing trabecular microstructural degeneration in osteoporotic rats.

J Orthop Res 2020 Dec 31. Epub 2020 Dec 31.

Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Receptor activator of nuclear factor kappa-B ligand (RANKL) is one of the key factors regulating the maturation of osteoclasts and an important target for osteoporosis treatment. A monoclonal antibody against RANKL showed effective therapeutic activity against osteoporosis by inhibiting bone resorption by osteoclasts. However, being an exogenous protein, its efficacy decreases after long-term use, and its discontinuation increases the risk of vertebral fractures. Here, we aimed to design an active immunotherapeutic agent to induce a T-cell dependent primary response. The agent, a mutant RANKL vaccine (mRv), was produced by cross-linking mutant RANKL, lacking the ability to stimulate osteoclast maturation, with the carrier protein keyhole limpet hemocyanin, a neo-antigen with a large molecular mass. Subcutaneous injection of mRv stimulated rats with ovariectomy-induced osteoporosis to produce high titers of anti-RANKL antibodies. The mutant RANKL vaccine decreased serum CTX-1 and BALP levels and inhibited the microstructural degeneration of trabecular bone in osteoporotic rats. mRv overcame immune system tolerance, stimulated rats to produce therapeutic antibodies, stabilized bone metabolism, and inhibited trabecular microstructural degeneration. These findings confirm the potential of the mutant RANKL vaccine to be developed into an effective preventive and therapeutic agent for osteoporosis.
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http://dx.doi.org/10.1002/jor.24980DOI Listing
December 2020

The pro-migration and anti-apoptosis effects of HMGA2 in HUVECs stimulated by hypoxia.

Cell Cycle 2020 12 14;19(24):3534-3545. Epub 2020 Dec 14.

Department of Cardiology, Renmin Hospital of Wuhan University , Wuhan, RP China.

High-mobility group AT-hook2 (HMGA2), serving as an architectural transcription factor, participates in plenty of biological processes. Our study is aimed at illustrating the effect of HMGA2 on hypoxia-induced HUVEC injury and the underlying mechanism. To induce hypoxia-related cell injury, HUVECs were exposed to hypoxic condition for 12-24 h. Molecular expression was determined by Western blot analysis, real-time PCR and immunofluorescence staining. Cell migration was monitored by wound healing assay and Transwell chamber assay. Cell proliferation and apoptosis were measured by MTT assay kits and TUNEL staining. In this study, we discovered that HMGA2 was upregulated in hypoxia-induced HUVECs. Overexpression of HMGA2 promoted cell migration, decreased the apoptosis ratio in response to hypoxia stimulation, while HMGA2 knockdown inhibited cell migration and accelerated apoptosis in HUVECs under hypoxic condition. Mechanistically, we found that HMGA2 induced increased expression of HIF-1α,VEGF, eNOS and AKT. eNOS knockdown significantly reduced HMGA2-mediated pro-migration effects, and AKT knockdown strikingly counteracted HMGA2-mediated anti-apoptotic effect. Hence, our data indicated that HMGA2 promoted cell migration by regulating HIF-1α/VGEF/eNOS signaling and prevented cell apoptosis by activating HIF-1α/VGEF/AKT signaling in HUVECs.
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http://dx.doi.org/10.1080/15384101.2020.1850970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781619PMC
December 2020

3D bioprinting of a biomimetic meniscal scaffold for application in tissue engineering.

Bioact Mater 2021 Jun 30;6(6):1711-1726. Epub 2020 Nov 30.

Medical School of Chinese PLA, Beijing, 100853, China.

Appropriate biomimetic scaffolds created via 3D bioprinting are promising methods for treating damaged menisci. However, given the unique anatomical structure and complex stress environment of the meniscus, many studies have adopted various techniques to take full advantage of different materials, such as the printing combined with infusion, or electrospining, to chase the biomimetic meniscus, which makes the process complicated to some extent. Some researchers have tried to tackle the challenges only by 3D biopringting, while its alternative materials and models have been constrained. In this study, based on a multilayer biomimetic strategy, we optimized the preparation of meniscus-derived bioink, gelatin methacrylate (GelMA)/meniscal extracellular matrix (MECM), to take printability and cytocompatibility into account together. Subsequently, a customized 3D bioprinting system featuring a dual nozzle + multitemperature printing was used to integrate the advantages of polycaprolactone (PCL) and meniscal fibrocartilage chondrocytes (MFCs)-laden GelMA/MECM bioink to complete the biomimetic meniscal scaffold, which had the best biomimetic features in terms of morphology and components. Furthermore, cell viability, mechanics, biodegradation and tissue formation in vivo were performed to ensure that the scaffold had sufficient feasibility and functionality, thereby providing a reliable basis for its application in tissue engineering.
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http://dx.doi.org/10.1016/j.bioactmat.2020.11.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711190PMC
June 2021

S100A8/A9 in Myocardial Infarction: A Promising Biomarker and Therapeutic Target.

Front Cell Dev Biol 2020 12;8:603902. Epub 2020 Nov 12.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Myocardial infarction (MI), the main cause of cardiovascular-related deaths worldwide, has long been a hot topic because of its threat to public health. S100A8/A9 has recently attracted an increasing amount of interest as a crucial alarmin that regulates the pathogenesis of cardiovascular disease after its release from myeloid cells. However, the role of S100A8/A9 in the etiology of MI is not well understood. Here, we elaborate on the critical roles and potential mechanisms of S100A8/A9 driving the pathogenesis of MI. First, cellular source of S100A8/A9 in infarcted heart is discussed. Then we highlight the effect of S100A8/A9 heterodimer in the early inflammatory period and the late reparative period of MI as well as myocardial ischemia/reperfusion (I/R) injury. Moreover, the predictive value of S100A8/A9 for the risk of recurrence of cardiovascular events is elucidated. Therefore, this review focuses on the molecular mechanisms of S100A8/A9 in MI pathogenesis to provide a promising biomarker and therapeutic target for MI.
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http://dx.doi.org/10.3389/fcell.2020.603902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688918PMC
November 2020

Ubl4A is critical for mitochondrial fusion process under nutrient deprivation stress.

PLoS One 2020 19;15(11):e0242700. Epub 2020 Nov 19.

Department of Biology, Illinois Institute of Technology, Chicago, IL, United States of America.

Mitochondrial fusion and fission are dynamic processes regulated by the cellular microenvironment. Under nutrient starvation conditions, mitochondrial fusion is strengthened for energy conservation. We have previously shown that newborns of Ubl4A-deficient mice were more sensitive to starvation stress with a higher rate of mortality than their wild-type littermates. Ubl4A binds with the actin-related protein Arp2/3 complex to synergize the actin branching process. Here, we showed that deficiency in Ubl4A resulted in mitochondrial fragmentation and apoptosis. A defect in the fusion process was the main cause of the mitochondrial fragmentation and resulted from a shortage of primed Arp2/3 complex pool around the mitochondria in the Ubl4A-deficient cells compared to the wild-type cells. As a result, the mitochondrial fusion process was not undertaken quickly enough to sustain starvation stress-induced cell death. Consequently, fragmented mitochondria lost their membrane integrity and ROS was accumulated to trigger caspase 9-dependent apoptosis before autophagic rescue. Furthermore, the wild-type Ubl4A, but not the Arp2/3-binding deficient mutant, could rescue the starvation-induced mitochondrial fragmentation phenotype. These results suggest that Ubl4A promotes the mitochondrial fusion process via Arp2/3 complex during the initial response to nutrient deprivation for cell survival.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242700PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676689PMC
January 2021

Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling.

Mol Med Rep 2020 Dec 11;22(6):4848-4856. Epub 2020 Oct 11.

Department of Geriatrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Myocyte apoptosis and oxidative stress key critical roles in the process of doxorubicin (DOX)‑induced cardiotoxicity. However, how apoptosis and oxidative stress arise in DOX‑induced heart injury remains largely unknown. Cathepsin B (CTSB) is a typical lysosomal cysteine protease that is associated with apoptosis, inflammatory responses, oxidative stress and autophagy. The present study aimed to investigate the role of CTSB in DOX‑induced heart injury and its potential mechanism. H9C2 cells were infected with adenovirus or transfected with small interfering RNA to overexpress or knock down CTSB, respectively, and then stimulated with DOX. DOX induced increased CTSB expression levels in H9C2 cells. DOX‑induced cardiomyocyte apoptosis and oxidative stress were attenuated by CTSB knockdown but aggravated by CTSB overexpression in vitro. Mechanistically, the present study showed that CTSB activated the NF‑κB pathway in response to DOX. In summary, CTSB aggravated DOX‑induced H9C2 cell apoptosis and oxidative stress via NF‑κB signalling. CTSB constitutes a potential therapeutic target for the treatment of DOX‑induced cardiotoxicity.
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http://dx.doi.org/10.3892/mmr.2020.11583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646931PMC
December 2020

Precise genome modification in tomato using an improved prime editing system.

Plant Biotechnol J 2021 03 25;19(3):415-417. Epub 2020 Nov 25.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

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http://dx.doi.org/10.1111/pbi.13497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955883PMC
March 2021

Identifying the Biological Characteristics Associated with Oviposition Behavior of Tea Leafhopper Matsuda Using the Blue Light Detection Method.

Insects 2020 Oct 16;11(10). Epub 2020 Oct 16.

Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.

Tea leafhopper ( Matsuda) is amongst the key pests in tea plantations around the East Asian region. Stereomicroscopy is a conventional method used for detecting tea leafhopper eggs by dissecting the tender tissues. However, there is a need for a faster and more efficient method to directly observe and investigate intact eggs within tea shoots. The absence of a proven method limits research efforts for determining the oviposition behavior of E. onukii. Herein, we applied the blue light detection method (BLDM), a technique recently developed for other species, in order to detect eggs directly and non-destructively within the tender shoot. In addition, we compared BLDM against the traditional stereomicroscope detection method (SMDM) for four tea cultivars. Notably, our results revealed that BLDM was precise and effective in measuring the egg laying quantity of on intact tea shoots. Neither tea cultivars nor egg density in the tender shoot significantly affected the accuracy of BLDM. Furthermore, biological characteristics that have rarely been reported previously for E. onukii were investigated using the BLDM, including zygote duration, ovipositional rhythm, egg distribution within the tender shoot, and in different leaf positions, numbers of eggs laid by a single female daily, and laid by the entire generation. Therefore, these findings provide insights into the basic and theoretical evidence for the strategy and mechanism associated with the oviposition behavior of .
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http://dx.doi.org/10.3390/insects11100707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602796PMC
October 2020

[Clinical Effectiveness of Decitabine Combined with Reduced FLAG Regimen in the Treatment of Senile Patients with High-Risk Acute Myeloid Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Oct;28(5):1440-1444

Department of Hematology, Tianjin First Central Hospital, The First Central Clinical College of Tianjin Medical University, Tianjin 300192, China.

Objective: To explore the efficacy and adverse reactions of decitabine combined with reduction FLAG regimen on the senile patients with high-risk AML.

Methods: 12 senile patients with high-risk AML received decitabine combined with reduced FLAG regimen (decitabine 20 mg/m, intravenous drip, qd, d 1-5; fludarabine 30 mg/m, intravenous drip lasts 30 min, qd, d 3-6; Ara-C 1 g/m, intravenous drip, qd, d 3-6; and G-CSF 300 μg/d, subcu- taneous injection, d 2 to neutrophils reached the lowest return to>1.0×10/L) in our study. The efficacy and adverse reactions of this regimen were analyzed.

Results: 9 patients achieved complete remission(CR) after one course of decitabine combined with reduced FLAG regimen, 2 patients achieved partial remission (PR) and 1 patient reached a stable disease (SD). The overall response rate was 92%. The median follow-up period was 7.4 months ranged from 3 to 12 months. The median survival time for all patients was 6.4 months. The main treatment-related toxicities were myelosuppression and infection due to neutropenia. Severe non-hematologic toxicities were not observed in these patients, and there was no treatment-related mortality.

Conclusion: Decitabine combined with reduced FLAG regimen has a definite clinical efficacy in the treatment of senile patients with high-risk AML. This regimen, as induction remission regimen, can effectively improve the CR rate and reduce the adverse reactions. Therefore, it may be used as one of the preferred induction remission regimen to treat the senile patients with high-risk AML.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.05.002DOI Listing
October 2020

Targeting PTEN to regulate autophagy and promote the repair of injured neurons.

Brain Res Bull 2020 12 10;165:161-168. Epub 2020 Oct 10.

Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, China. Electronic address:

The effects of autophagy on neuronal damage can be positive or detrimental negative. Through establishing a model of fetal rat cortical neuron hydraulic shock injury, dipotassium bisperoxo (picolinoto) oxovanadate (V) [bpv(pic)] was used to inhibit PTEN at different time points post-injury and autophagy level after neuronal injury was assessed. Neurons were divided into several intervention groups according to the time point at which bpv(pic) was used to inhibit autophagy, normal neurons and injuried neurons were set as two control groups. Growth of neurons in each group was assessed through immunofluorescence staining. Expression of the autophagy-related proteins LC3-II and LC3-I was analyzed by western blot. Expression of PTEN, mTOR and Beclin-1 was detected by RT-PCR. The number of autophagosomes in the normal group, injury control group and 24 h, 36 h intervention groups were assessed by electron microscope. We found that autophagy was enhanced after neuronal injury and that the levels of LC3-II was significantly reduced by bpv (pic) intervention. The growth of the injury control groups was worse than normal groups, while improved through bpv(pic) intervention at 24 h and 30 h after injured. Western blot analysis showed that the LC3-II and LC3-II/LC3-I ratios of cells increased post-injury, and autophagy induction was evident by electron microscopy. These effects were confirmed by RT-PCR analysis. Taken together, these data suggest that autophagy is activated after injury in neurons while can be inhibited by bpv(pic) administration and then promote the repair of injured neurons.
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http://dx.doi.org/10.1016/j.brainresbull.2020.10.008DOI Listing
December 2020

Mesenchymal Stem Cell-Derived Exosomes: Hope for Spinal Cord Injury Repair.

Stem Cells Dev 2020 12 9;29(23):1467-1478. Epub 2020 Nov 9.

Department of Basic Medical Sciences, College of Veterinary Medicine, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.

Spinal cord injury (SCI) is a devastating medical condition with profound social and economic impacts. Although research is ongoing, current treatment options are limited and do little to restore functionality. However, recent studies suggest that mesenchymal stem cell-derived exosomes (MSC-exosomes) may hold the key to exciting new treatment options for SCI patients. MSCs are self-renewing multipotent stem cells with multi-directional differentiation and can secrete a large number of exosomes (vesicles secreted into the extracellular environment through endocytosis, called MSC-exosomes). These MSC-exosomes play a critical role in repairing SCI through promoting angiogenesis and axonal growth, regulating inflammation and the immune response, inhibiting apoptosis, and maintaining the integrity of the blood-spinal cord barrier. Furthermore, they can be utilized to transport genetic material or drugs to target cells, and their relatively small size makes them able to permeate the blood-brain barrier. In this review, we summarize recent advances in MSC-exosome themed SCI treatments and cell-free therapies to better understand this newly emerging methodology.
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http://dx.doi.org/10.1089/scd.2020.0133DOI Listing
December 2020

Practical Exponential Stability of Impulsive Stochastic Reaction-Diffusion Systems With Delays.

IEEE Trans Cybern 2020 Oct 1;PP. Epub 2020 Oct 1.

This article studies the practical exponential stability of impulsive stochastic reaction-diffusion systems (ISRDSs) with delays. First, a direct approach and the Lyapunov method are developed to investigate the pth moment practical exponential stability and estimate the convergence rate. Note that these two methods can also be used to discuss the exponential stability of systems in certain conditions. Then, the practical stability results are successfully applied to the impulsive reaction-diffusion stochastic Hopfield neural networks (IRDSHNNs) with delays. By the illustration of four numerical examples and their simulations, our results in this article are proven to be effective in dealing with the problem of practical exponential stability of ISRDSs with delays, and may be regarded as stabilization results.
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http://dx.doi.org/10.1109/TCYB.2020.3022024DOI Listing
October 2020

The value of intravoxel incoherent motion diffusion-weighted imaging in predicting the pathologic response to neoadjuvant chemotherapy in locally advanced esophageal squamous cell carcinoma.

Eur Radiol 2021 Mar 8;31(3):1391-1400. Epub 2020 Sep 8.

Department of Radiology, Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming road, Jinshui District, Zhengzhou city, Henan Province, China.

Objective: To explore the value of intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) for the prediction of pathologic response to neoadjuvant chemotherapy (NAC) in locally advanced esophageal squamous cell carcinoma (ESCC).

Material And Methods: Forty patients with locally advanced ESCC who were treated with NAC followed by radical resection were prospectively enrolled from September 2015 to May 2018. MRI and IVIM were performed within 1 week before and 2-3 weeks after NAC, prior to surgery. Parameters including apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudodiffusion coefficient (D), and pseudodiffusion fraction (f) before and after NAC were measured. Pathologic response was evaluated according to the AJCC tumor regression grade (TRG) system. The changes in IVIM values before and after therapy in different TRG groups were assessed. Receiver operating characteristic (ROC) curves analysis was used to determine the best cutoff value for predicting the pathologic response to NAC.

Results: Twenty-two patients were identified as TRG 2 (responders), and eighteen as TRG 3 (non-responders) in pathologic evaluation. The ADC, D, and f values increased significantly after NAC. The post-NAC D and ΔD values of responders were significantly higher than those of non-responders. The area under the curve (AUC) was 0.722 for post-NAC D and 0.859 for ΔD in predicting pathologic response. The cutoff values of post-NAC D and ΔD were 1.685 × 10 mm/s and 0.350 × 10 mm/s, respectively.

Conclusion: IVIM-DWI may be used as an effective functional imaging technique to predict pathologic response to NAC in locally advanced ESCC.

Key Points: • The optimal cutoff values of post-NAC D and ΔD for predicting pathologic response to NAC in locally advanced ESCC were 1.685 × 10 mm/s and 0.350 × 10 mm/s, respectively. • Pathologic response to NAC in locally advanced ESCC was favorable in patients with post-NAC D and ΔD values that were higher than the optimal cutoff values. • IVIM-DWI can potentially be used to preoperatively predict pathologic response to NAC in esophageal carcinoma. Accurate quantification of the D value derived from IVIM-DWI may eventually translate into an effective and non-invasive marker to predict therapeutic efficacy.
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http://dx.doi.org/10.1007/s00330-020-07248-zDOI Listing
March 2021

Nucleotide-Binding Oligomerization Domain-Like Receptor 3 Deficiency Attenuated Isoproterenol-Induced Cardiac Fibrosis via Reactive Oxygen Species/High Mobility Group Box 1 Protein Axis.

Front Cell Dev Biol 2020 11;8:713. Epub 2020 Aug 11.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) is involved in fibrosis of multiple organs, such as kidney, liver, lung, and the like. However, the role of NLRP3 in cardiac fibrosis is still controversial and remains unclear. The study aims to investigate the role of NLRP3 on cardiac fibrosis induced by isoproterenol (ISO). , NLRP3 knockout and wild-type mice were subcutaneously injected with ISO to induce the cardiac fibrosis model. The results showed that NLRP3 deficiency alleviated the cardiac fibrosis and inflammation induced by ISO. , neonatal rat ventricular myocytes (NRVMs) and primary adult mouse cardiac fibroblasts of NLRP3 knockout and wild-type mice were isolated and challenged with ISO. Adenovirus (Ad-) NLRP3 and small interfering RNAs targeting NLRP3 were used to transfect NRVMs to overexpress or knockdown NLRP3. We found that NLRP3 could regulate high-mobility group box 1 protein (HMGB1) secretion via reactive oxygen species production in NRVMs and the HMGB1 secreted by NRVMs promoted the activation and proliferation of cardiac fibroblasts. Thus, we concluded that the NLRP3/reactive oxygen species/HMGB1 pathway could be the underlying mechanism of ISO-induced cardiac fibrosis.
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http://dx.doi.org/10.3389/fcell.2020.00713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431462PMC
August 2020

A Structural Model for Bax∆2-Mediated Activation of Caspase 8-Dependent Apoptosis.

Int J Mol Sci 2020 Jul 31;21(15). Epub 2020 Jul 31.

Department of Chemistry, Illinois Institute of Technology, Chicago, IL 60616, USA.

Bax∆2 is a pro-apoptotic anti-tumor protein in the Bax family. While most of the Bax family causes cell death by targeting mitochondria, Bax∆2 forms cytosolic aggregates and activates caspase 8-dependent cell death. We previously showed that the Bax∆2 helix α9 is critical for caspase 8 recruitment. However, the interaction between these two proteins at the structural level is unknown. In this in silico study, we performed molecular dynamics (MD) simulations and protein-protein docking on Bax∆2 variants. The results suggest that the Bax∆2 variants have different stable states. Mutating the Baxα mitochondria-targeting signal [L26P/L27P] appears to introduce a kink into helix α1. Protein-protein docking suggests that helices α9 of both wild-type Bax∆2 and Bax∆2 caspase 8 binding-deficient mutant [L164P] can fit in the same caspase 8 binding site, but the mutant is unable to fit as well as wild-type Bax∆2. Together, these data point to a structural basis for explaining Bax∆2 function in caspase 8-dependent cell death.
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http://dx.doi.org/10.3390/ijms21155476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432750PMC
July 2020

Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease.

Exp Ther Med 2020 Aug 15;20(2):1021-1029. Epub 2020 May 15.

Department of Geriatrics, Jiangsu Provincial Key Laboratory of Geriatrics, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.

Parkinson's disease (PD) is a chronic progressive disease that affects the central nervous system with a variety of symptoms. Although the precise etiology of PD is not yet fully understood, there is evidence to suggest that T cells serve an important role in the pathogenesis of PD. However, how T cells are recruited in the brain tissue remains to be elucidated. The present study utilized human samples from patients with and without PD to investigate the infiltration of T cells in lesions in the central nervous system. A chemically-induced mouse PD model was also used to investigate the roles of T cells in the pathogenesis of PD. Depletion of CD4 or CD8 T cells was achieved using neutralizing antibodies. Adhesion molecule levels were assessed by flow cytometry. The results of the study indicated that T cell infiltration was evident in both human and murine samples of PD. Blocking CD4 or CD8 T cells attenuated the severity of murine PD. Intercellular adhesion molecule 1 (ICAM1 or CD54) was upregulated in mouse PD compared with controls, and its receptor, lymphocyte function-associated antigen-1 (LFA1) was overexpressed in T cells of the brain in PD mice compared with controls. Furthermore, inhibition of ICAM1 or LFA1 attenuated PD-associated characteristics in mice. In conclusion, the interaction between ICAM1 and LFA1 plays a role in recruiting T cells to the central nervous system to mediate experimental PD.
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http://dx.doi.org/10.3892/etm.2020.8758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388384PMC
August 2020
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