Publications by authors named "Qi Wu"

1,456 Publications

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Clinical characteristics of 310 SARS-CoV-2 Omicron variant patients and comparison with Delta and Beta variant patients in China.

Virol Sin 2022 Aug 7. Epub 2022 Aug 7.

Department of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address:

Although the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant has spread, data on the clinical characteristics of infected patients are limited. In this study, the demographic, clinical characteristics, and laboratory data of 310 SARS-CoV-2 Omicron variant patients treated at Haihe Hospital of Tianjin were collected and analyzed. Information on these patients was compared to 96 patients with the Delta variant of concern (VOC) and 326 patients with the Beta VOC during the previous coronavirus disease 2019 (COVID-19) outbreak in Harbin. Of the 310 patients infected with the Omicron variant, the median age was 35 years. Most patients were clinically classified as mild (57.74%), and the most common symptoms were cough (48.71%), fever (39.35%), and sore throat (38.26%). The results for different vaccination groups in the Omicron group showed that the median of "SARS-CoV-2 specific IgG" after 2 or 3 doses of vaccination was higher than the unvaccinated group (all Ps < 0.05). Older age was associated with a higher proportion of moderate cases and lower asymptomatic and mild cases based on clinical classifications. Compared to the Delta and Beta groups, the median age of the Omicron group was younger. The total number of asymptomatic patients and mild patients in the Omicron virus group was higher than the Delta and Beta groups (60.97% vs. 54.17% vs. 47.55%). This study presented the clinical characteristics of the first group of patients infected with the Omicron variant in Tianjin, China, and compared their clinical features with patients infected by the Delta and Beta variants, which would increase our understanding of the characteristics of SARS-CoV-2 Omicron variant.
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http://dx.doi.org/10.1016/j.virs.2022.07.014DOI Listing
August 2022

Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.

Int J Biol Macromol 2022 Aug 5. Epub 2022 Aug 5.

Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address:

Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption. In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III). The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action. The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD. Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes. In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention. Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on. Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism. The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased. Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver. These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.211DOI Listing
August 2022

Integrated network analyses identify MYB4R1 neofunctionalization in the UV-B adaptation of tartary buckwheat.

Plant Commun 2022 Aug 2:100414. Epub 2022 Aug 2.

College of Life Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address:

A hallmark of adaptive evolution is innovation in gene function, which is associated with the development of distinct roles for genes in plant evolution; however, assessing functional innovation over a long time is not trivial. Tartary buckwheat (F. tataricum) originated in the Himalayan region and has been exposed to intense UV-B radiation for a long time, making it an ideal species for studying novel UV-B response mechanisms in plants. Here, we developed a workflow to report a co-functional network of UV-B responses using data from more than 10,000 samples of more than 80 projects with multi-species and multi-omics data. Dissecting the entire network revealed that flavonoid biosynthesis is most significantly related to the UV-B response. Importantly, we found that the regulatory factor MYB4R1, which resides at the core of the network, undergoes neofunctionalization. In vitro and in vivo experiments demonstrated that MYB4R1 regulates flavonoid and anthocyanin accumulation in response to UV-B in buckwheat by binding to L box motifs in the FtCHS, FtFLS, and FtUFGT promoters, and we used deep learning to develop a visual discrimination model of buckwheat flavonoid content based on natural populations exposed to global UV-B radiation. Our study highlights the critical role of gene neofunctionalization in UV-B adaptation.
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http://dx.doi.org/10.1016/j.xplc.2022.100414DOI Listing
August 2022

Salicylic Acid Acts Upstream of Auxin and Nitric Oxide (NO) in Cell Wall Phosphorus Remobilization in Phosphorus Deficient Rice.

Rice (N Y) 2022 Aug 3;15(1):42. Epub 2022 Aug 3.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, 210008, China.

Salicylic acid (SA) is thought to be involved in phosphorus (P) stress response in plants, but the underlying molecular mechanisms are poorly understood. Here, we showed that P deficiency significantly increased the endogenous SA content by inducing the SA synthesis pathway, especially for up-regulating the expression of PAL3. Furthermore, rice SA synthetic mutants pal3 exhibited the decreased root and shoot soluble P content, indicating that SA is involved in P homeostasis in plants. Subsequently, application of exogenous SA could increase the root and shoot soluble P content through regulating the root and shoot cell wall P reutilization. In addition, - P + SA treatment highly upregulated the expression of P transporters such as OsPT2 and OsPT6, together with the increased xylem P content, suggesting that SA also participates in the translocation of the P from the root to the shoot. Moreover, both signal molecular nitric oxide (NO) and auxin (IAA) production were enhanced when SA is applied while the addition of respective inhibitor c-PTIO (NO scavenger) and NPA (IAA transport inhibitor) significantly decreased the root and shoot cell wall P remobilization in response to P starvation. Taken together, here SA-IAA-NO-cell wall P reutilization pathway has been discovered in P-starved rice.
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http://dx.doi.org/10.1186/s12284-022-00588-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349334PMC
August 2022

Feasibility of FDCT Early Brain Parenchymal Blood Volume Maps in Predicting Short-Term Prognosis in Patients With Aneurysmal Subarachnoid Hemorrhage.

Front Neurol 2022 14;13:888369. Epub 2022 Jul 14.

Department of Neurosurgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.

Purpose: Aneurysmal subarachnoid hemorrhage (SAH) is accompanied by cerebral perfusion changes. We aimed to measure the parenchymal blood volume (PBV) maps acquired by C-arm flat-panel detector CT (FDCT) to assess the cerebral blood volume at an early stage in aneurysmal SAH and to explore the correlation with the outcomes at discharge.

Methods: Data of 66 patients with aneurysmal SAH who underwent FDCT PBV examination were retrospectively analyzed. The PBV of regions of interest, including the cortices of the bilateral frontal lobe, the parietal lobe, the occipital lobe, and the cerebral hemisphere, as well as the basal ganglia, were measured and quantitatively analyzed. The clinical and imaging data of the patients were also collected, and logistic regression analysis was performed to explore the correlation between the perfusion parameters and outcomes at discharge.

Results: The favorable and poor outcomes at discharge were found in 37 (56.06%) and 29 (43.94%) patients, respectively. The whole-brain PBV was significantly correlated with the Hunt-Hess grades ( < 0.005) and the WFNSS grades ( < 0.005). The whole-brain PBV of the poor prognosis was significantly higher than that of the favorable prognosis (35.17 ± 7.66 vs. 29.78 ± 5.54, < 0.005). The logistic regression analysis showed that the PBV of the parietal lobe at the bleeding side (OR = 1.10, 95%CI: 1.00-1.20, = 0.04) was an independent risk factor predicting the short-term prognosis.

Conclusions: Parenchymal blood volume (PBV) maps could reflect the cerebral blood volume throughout the brain to characterize its perfusion status at an early stage in aneurysmal SAH. It enables a one-stop imaging evaluation and treatment in the same angio-suite and may serve as a reliable technique in clinical assessment of aneurysmal SAH.
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http://dx.doi.org/10.3389/fneur.2022.888369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329812PMC
July 2022

Inhibition of acylglycerol kinase sensitizes DLBCL to venetoclax via upregulation of FOXO1-mediated BCL-2 expression.

Theranostics 2022 18;12(12):5537-5550. Epub 2022 Jul 18.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan, China.

Despite of the paradigm change on the treatments of acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL) by venetoclax, it has been less successful in the treatment of diffuse large B-cell lymphoma (DLBCL). Here, we explored whether acylglycerol kinase regulates the sensitivity of DLBCLs to venetoclax and its mechanism in both cell lines and preclinical animal models. The expression of AGK and sensitivity to venetoclax of seven DLBCL cell lines were determined. Upon knockdown and overexpression of AGK by lentivirus in DLBCL cells, the venetoclax-induced apoptosis and PTEN-FOXO1-BCL-2 signaling axis were evaluated . The efficacy of venetoclax and PTEN-FOXO1-BCL-2 signaling axis were evaluated in immunodeficient NCG mice that were implanted with control or shAGK stably transduced SU-DHL4 cells. The expressions of AGK, BCL-2 and FOXO1 were evaluated in tumor tissues of DLBCL patients. AGK expression was inversely correlated with sensitivity of DLBCL to venetoclax. Inhibition of AGK rendered the DLBCL cells more sensitive to venetoclax. Mechanistically, AGK phosphorylated and inactivated PTEN, which led to AKT activation and reduced FOXO1 nuclear translocation. Inhibition of AGK also led to enhanced efficacy of venetoclax for suppression of DLBCL tumor growth , which was dependent on FOXO1. In human DLBCL tumor tissues, the expression of AGK inversely correlated with BCL-2 expression, as well as the amounts of nuclear FOXO1. Our data demonstrated that AGK regulates venetoclax response in DLBCL via PTEN-FOXO1-BCL-2 signaling axis. Targeting AGK may enhance the efficacy of venetoclax for the treatment of DLBCL patients.
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http://dx.doi.org/10.7150/thno.72786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330532PMC
August 2022

EEG-FCV: An EEG-Based Functional Connectivity Visualization Framework for Cognitive State Evaluation.

Front Psychiatry 2022 11;13:928781. Epub 2022 Jul 11.

College of Computer and Technology, Hangzhou Dianzi University, Hangzhou, China.

Electroencephalogram (EEG)-based tools for brain functional connectivity (FC) analysis and visualization play an important role in evaluating brain cognitive function. However, existing similar FC analysis tools are not only visualized in 2 dimensions (2D) but also are highly prone to cause visual clutter and unable to dynamically reflect brain connectivity changes over time. Therefore, we design and implement an EEG-based FC visualization framework in this study, named EEG-FCV, for brain cognitive state evaluation. EEG-FCV is composed of three parts: the Data Processing module, Connectivity Analysis module, and Visualization module. Specially, FC is visualized in 3 dimensions (3D) by introducing three existing metrics: Pearson Correlation Coefficient (PCC), Coherence, and PLV. Furthermore, a novel metric named Comprehensive is proposed to solve the problem of visual clutter. EEG-FCV can also visualize dynamically brain FC changes over time. Experimental results on two available datasets show that EEG-FCV has not only results consistent with existing related studies on brain FC but also can reflect dynamically brain FC changes over time. We believe EEG-FCV could prompt further progress in brain cognitive function evaluation.
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http://dx.doi.org/10.3389/fpsyt.2022.928781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309393PMC
July 2022

Thermal aggregation behavior of egg white protein and blue round scad (Decapterus maruadsi) myofibrillar protein.

J Food Sci 2022 Jul 27. Epub 2022 Jul 27.

National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, National R&D Branch Center of Surimi and Surimi Products Processing, National and Local United Engineering Lab of Marine Functional Food, Collaborative Innovation Center of Seafood Deep Processing, College of Food Science and Technology, Bohai University, Jinzhou, China.

In the present study, egg white protein (EWP) and myofibrillar protein (MP) were mixed in different ratios (0/100, 10/90, 20/80, 30/70, 40/60, 50/50, 100/0 for EWP/MP) and subjected to unheated, preheated (40°C/30 min), two-step heated (40°C/30 min, 90°C/20 min), and one-step heated (90°C/20 min) treatments to study the thermal aggregation of the two proteins. Their aggregation behavior was characterized by turbidity, active sulfhydryl, degree of protein cross-linking, protein characteristic spectra, and microscopic morphology. The results indicated that for the mixed protein system composed of EWP and MP, the mixed protein aggregation volume was larger and regular at an EWP/MP of 30/70, when the degree of cross-linking was best. When the ratio of EWP/MP was 50/50, the aggregate-protein interaction was dominant, and the excess EWP acted as a barrier to cross-linking and wrapped around the surface of the aggregates to form larger aggregates. Comparing the two-step heated and one-step heated conditions, the former is superior. PRACTICAL APPLICATION: The combination of egg white protein and myofibrillar protein can provide a theoretical reference for the protein content in surimi products, and moderate addition has an enhancing effect on surimi protein cross-linking and promotes gel formation. Excessive addition will form aggregates outside the egg white protein wrapping phenomenon, and the quality of surimi gel products will be affected.
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http://dx.doi.org/10.1111/1750-3841.16255DOI Listing
July 2022

Iron deficiency promotes aortic media degeneration by activating endoplasmic reticulum stress-mediated IRE1 signaling pathway.

Pharmacol Res 2022 Jul 23;183:106366. Epub 2022 Jul 23.

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, 238# Jiefang Road, Wuhan 430000, Hubei Province, China; Cardiovascular Surgery Laboratory, Renmin Hospital of Wuhan University, 9# Zhangzhidong Road, Wuhan 430000, Hubei Province, China; Central Laboratory, Renmin Hospital of Wuhan University, 9# Zhangzhidong Road, Wuhan 430000, Hubei Province, China.

Background: Aortic dissection (AD) is a macrovascular disease which is pathologically characterized by aortic media degeneration (AMD). Our team's previous research found that iron deficiency (ID) promoted the formation of AMD through presentative research. In this study, we aimed to investigate the underlying mechanism of ID promoting AMD formation.

Methods: The human aortic tissues were harvested from AD patients and organ donors. ApoE-/- mice were simultaneously given AngII infusion and low-iron feed to investigate the relationship between ID and AD. The IRE1-XBP1-CHOP signal axis of endoplasmic reticulum (ER) stress was selectively inhibited with 4μ8C. Iron contents were detected by Perls staining. The expression of iron metabolism and ER stress-relative proteins were analyzed by IF and western blotting. Apoptosis rates of aortic tissue and ASMCs were detected by TUNEL staining and flow cytometry, and ROS content was also measured by the flow cytometry.

Results: ID was accompanied by ER stress in patients with AD. Among the three signaling pathways of ER stress in ID-induced AMD, proteins of IRE1, PERK and ATF6 signaling pathways were up-regulated by 2.65 times, 1.14 times and 1.24 times, respectively. ID was positively related to ER stress, mitochondrial oxidative stress and aortic media apoptosis in vivo and in vitro assays, while 4μ8C reversed the severity of ER stress and AMD.

Conclusions: ID could activate ER stress by eliciting mitochondrial oxidative stress to activate the IRE1-XBP1-CHOP signaling pathway in the ER, which accelerated the apoptosis of ASMCs in aortic media, thus promoting the formation of AMD.
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http://dx.doi.org/10.1016/j.phrs.2022.106366DOI Listing
July 2022

Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics.

Front Cell Dev Biol 2022 7;10:891731. Epub 2022 Jul 7.

Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Uncoupling protein 1 (UCP1) and UCP2 are associated with tumor metabolism and immunity. However, the prognostic value and molecular mechanisms underlying their action in breast cancer (BC) remain unclear. In TCGA-BRCA cohort, we investigated the expression characteristics of UCP mRNAs, analyzed their prognostic value by Kaplan-Meier survival analysis, their potential molecular functions by gene set enrichment analysis, and their relationship with immune infiltrating cell types using TIMER and CIBERSORT, along with the assessment of their association with mutational profiles. Kaplan-Meier survival analysis was performed for UCPs in our cohort and their association with BC thermogenesis was assessed by thermal tomography. High expression of UCP1 and UCP2 were positive prognostic markers for BC. UCP1 was associated with the impaired glucose metabolism, while UCP2 with enhanced anti-tumor immunity. High expressions of UCP1 and UCP2 were associated with CDH1 mutations. High UCP1 expression was associated with a high rate of thermogenesis in BC. These results implied a key role of UCP1 and UCP2 in prognosis, metabolism, and immune infiltration in BC. Further investigation of the relevant molecular mechanisms may provide new strategies for BC treatment.
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http://dx.doi.org/10.3389/fcell.2022.891731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300932PMC
July 2022

Dietary regulation in health and disease.

Signal Transduct Target Ther 2022 07 23;7(1):252. Epub 2022 Jul 23.

Tongji University Cancer Center, Shanghai Tenth People's Hospital of Tongji University, School of Medicine, Tongji University, Shanghai, 200092, China.

Nutriments have been deemed to impact all physiopathologic processes. Recent evidences in molecular medicine and clinical trials have demonstrated that adequate nutrition treatments are the golden criterion for extending healthspan and delaying ageing in various species such as yeast, drosophila, rodent, primate and human. It emerges to develop the precision-nutrition therapeutics to slow age-related biological processes and treat diverse diseases. However, the nutritive advantages frequently diversify among individuals as well as organs and tissues, which brings challenges in this field. In this review, we summarize the different forms of dietary interventions extensively prescribed for healthspan improvement and disease treatment in pre-clinical or clinical. We discuss the nutrient-mediated mechanisms including metabolic regulators, nutritive metabolism pathways, epigenetic mechanisms and circadian clocks. Comparably, we describe diet-responsive effectors by which dietary interventions influence the endocrinic, immunological, microbial and neural states responsible for improving health and preventing multiple diseases in humans. Furthermore, we expatiate diverse patterns of dietotheroapies, including different fasting, calorie-restricted diet, ketogenic diet, high-fibre diet, plants-based diet, protein restriction diet or diet with specific reduction in amino acids or microelements, potentially affecting the health and morbid states. Altogether, we emphasize the profound nutritional therapy, and highlight the crosstalk among explored mechanisms and critical factors to develop individualized therapeutic approaches and predictors.
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http://dx.doi.org/10.1038/s41392-022-01104-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308782PMC
July 2022

Synthesis of Patchy Nanoparticles with Symmetry Resembling Polar Small Molecules.

Small Methods 2022 Jul 22:e2200545. Epub 2022 Jul 22.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University Shanghai, Shanghai, 200438, P. R. China.

Patchy nanoparticles (NPs) show many important applications, especially for constructing structurally complex colloidal materials, but existing synthetic strategies generate patchy NPs with limited types of symmetry. This article describes a versatile copolymer ligand-based strategy for the scalable synthesis of uniform Au-(SiO ) patchy NPs (x is the patch number and 1 ≤ x ≤ 5) with unusual symmetry at high yield. The hydrolysis and condensation of tetraethyl orthosilicate on block-random copolymer ligands induces the segregation of copolymers on gold NPs (AuNPs) and hence governs the structure and distribution of silica patches formed on the AuNPs. The resulting patchy NPs possess unique configurations where the silica patches are symmetrically arranged at one side of the core NP, resembling the geometry of polar small molecules. The number, size, and morphology of silica patches, as well as the spacing between the patches and the AuNP can be precisely tuned by tailoring copolymer architectures, grafting density of copolymers, and the size of AuNPs. Furthermore, it is demonstrated that the Au-(SiO ) patchy NPs can assemble into more complex superstructures through directional interaction between the exposed Au surfaces. This work offers new opportunities of designing next-generation complex patchy NPs for applications in such as biomedicines, self-assembly, and catalysis.
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http://dx.doi.org/10.1002/smtd.202200545DOI Listing
July 2022

Clinical efficacy and safety of NSCLC ancillary treatment with compound Kushen injection through immunocompetence regulation: A systematic review and meta-analysis.

Phytomedicine 2022 Sep 6;104:154315. Epub 2022 Jul 6.

Department of Physiology, Xuzhou Medical University, Xuzhou, 221009, China. Electronic address:

Background: Compound Kushen injection (CKI) is a Chinese patented medicine that improves the immunity level of cancer patients and inhibits tumor cell proliferation and metastasis. Clinically, CKI is widely used in combination with platinum-based chemotherapy (PBC) for non-small cell lung cancer (NSCLC) treatment. This study attempted to systemically evaluate the efficacy and safety of a combination of CKI and PBC for NSCLC treatment by modulating the immune function.

Purpose: To evaluate the clinical efficacy and safety of CKI in combination with PBC for NSCLC.

Materials And Methods: English and Chinese databases were retrieved for randomized controlled trials (RCTs) of NSCLC treatment using a combination of CKI and PBC, and the changes of peripheral blood T lymphocytes (such as CD3 T cells, CD4 T cells, CD8 T cells), and CD4/CD8 T cell ratio among NSCLC patients were detected before and after treatment using CKI with PBC. The search deadline was set as November 2021. The systemic evaluation was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The methodology and quality of each study included in the systemic evaluation were assessed. Review Manager 5.4, Stata12.0, and trial sequential analysis (TSA) were used for data analysis. The outcome indicators were qualified using GRADEprofiler software.

Results: A total of 25 RCTs involving 2460 cases of patients were included. The results showed that the combination of CKI with PBC effectively increased the objective response rate (ORR) [relative risk (RR) = 1.31, 95% confidence interval (CI) (1.19, 1.44)] and disease control rate (DCR) [RR = 1.16, 95%CI (1.09,1.23)], regulated the expression of peripheral blood T lymphocytes (such as CD3T cells, CD4T cells, CD8T cells, and CD4/CD8T cell ratio), upregulated the level of serum immunoglobulins (such as IgA, IgG, and IgM), and reduced the frequency of gastrointestinal reaction, marrow inhibition, hepatorenal toxicity, reduction of white blood cells and blood platelets, baldness, infection, neutrophilic granulocyte counts, diarrhea, or constipation. According to subgroup analysis results, chemotherapy cycles (1-2) had a more significant effect on DCR. A combination of CKI and GP regimens had better effects on improving CD3T cell levels, and there were no significant changes among other chemotherapies regiments.

Conclusion: A combination of CKI and PBC had a marked effect in improving tumor response, priming immune function, and decreasing the frequency of adverse reactions, which was safe for NSCLC treatment.
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http://dx.doi.org/10.1016/j.phymed.2022.154315DOI Listing
September 2022

Corrigendum to "Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake" [Curr. Res. Food Sci. 5(2022) 515-530].

Curr Res Food Sci 2022 14;5:1108-1109. Epub 2022 Jul 14.

Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China.

[This corrects the article DOI: 10.1016/j.crfs.2022.02.013.].
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http://dx.doi.org/10.1016/j.crfs.2022.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294490PMC
July 2022

Artificial intelligence-assisted colonoscopy for colorectal cancer screening: a multicenter randomized controlled trial.

Clin Gastroenterol Hepatol 2022 Jul 18. Epub 2022 Jul 18.

Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China; Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong SAR, China; Stanley Ho Big Data Decision Analytics Research Centre, The Chinese University of Hong Kong, Hong Kong SAR, China; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore. Electronic address:

Background: Artificial intelligence (AI)-assisted colonoscopy improves polyp detection and characterization in colonoscopy. However, data from large scale multicenter randomized controlled trials (RCT) in an asymptomatic population are lacking.

Methods: This multicenter RCT aimed to compare AI-assisted colonoscopy (AI) to conventional colonoscopy (CC) for adenoma detection in an asymptomatic population. Asymptomatic subjects aged 45 to 75 years undergoing colorectal cancer screening by direct colonoscopy or fecal immunochemical test (FIT) were recruited in 6 referral centers in Hong Kong, Jilin, Inner Mongolia, Xiamen and Beijing. In the AI group, an AI polyp detection system (Eagle-Eye, Xiamen InnoVision) with real time notification on the same monitor of the endoscopy system was used. Primary outcome was overall adenoma detection rate (ADR). Secondary outcomes were mean number of adenomas per colonoscopy (APC), ADR according to endoscopist's experience, and colonoscopy withdrawal time. This study received IRB approval (CRE-2019.393).

Results: From November 2019 to August 2021, 3059 subjects were randomized to AI (1519) and CC (1540). Baseline characteristics and bowel preparation quality between the two groups were similar. The overall ADR (39.9% vs 32.4%, p<0.001), Advanced ADR (6.6% vs 4.9%, P=0.041), ADR of expert (42.3% vs 32.8%, p<0.001) and non-expert endoscopists (37.5% vs 32.1%, p=0.023) and APC (0.59+/-0.97 vs 0.45+/-0.81, p<0.001) were all significantly higher in AI group. The median withdrawal time (minutes) (8.3 vs 7.8, p=0.004) was slightly longer in AI group.

Conclusion: In this multicenter RCT in asymptomatic patients, AI-assisted colonoscopy improved overall ADR, advanced ADR and ADR of both expert and non-expert attending endoscopists.
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http://dx.doi.org/10.1016/j.cgh.2022.07.006DOI Listing
July 2022

Bioinspired Design of High Vibration-Damping Supramolecular Elastomers Based on Multiple Energy-Dissipation Mechanisms.

ACS Appl Mater Interfaces 2022 Aug 20;14(30):35097-35104. Epub 2022 Jul 20.

State Key Laboratory of Polymer Materials Engineering, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.

Suppressing vibrations and noises is essential for our automated society. Here, inspired by the hierarchical dynamic bonds and phase separation of mussel byssal threads, we synthesize high-damping supramolecular elastomers (HDEs) via simple one-pot radical polymerization of butyl acrylate (BA), acrylic acid (AA), and vinylimidazole (VI). Interestingly, AA and VI not only form hydrogen bonds and ionic bonds simultaneously but also segregate into aggregates of different sizes, thereby successfully mimicking the hierarchical structure of mussel byssal threads. When applying external forces, the weak hydrogen bonds are broken at first and then the ionic bonds and aggregates are disrupted progressively from small to large deformations. Such multiple energy-dissipation mechanisms lead to the outstanding damping property of the HDEs. Therefore, the HDEs outperform commercially available rubbers in terms of sound absorption and vibration damping. Furthermore, the multiple energy-dissipation mechanisms impart the HDEs with high toughness (41.1 MJ/m), tensile strength (21.3 MPa), and self-healing ability.
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http://dx.doi.org/10.1021/acsami.2c07604DOI Listing
August 2022

Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage.

Oxid Med Cell Longev 2022 9;2022:9069825. Epub 2022 Jul 9.

Department of Neurosurgery, Jinling Hospital, Nanjing Medical University, Nanjing, 210002 Jiangsu, China.

Ferroptosis is a regulated cell death that characterizes the lethal lipid peroxidation and iron overload, which may contribute to early brain injury (EBI) pathogenesis after subarachnoid hemorrhage (SAH). Although Sirtuin 1 (SIRT1), a class III histone deacetylase, has been proved to have endogenous neuroprotective effects on the EBI following SAH, the role of SIRT1 in ferroptosis has not been studied. Hence, we designed the current study to determine the role of ferroptosis in the EBI and explore the correlation between SIRT1 and ferroptosis after SAH. The pathways of ferroptosis were examined after experimental SAH (prechiasmatic cistern injection mouse model) and in HT-22 cells stimulated by oxyhemoglobin (oxyHb) . Then, ferrostatin-1 (Fer-1) was used further to determine the role of ferroptosis in EBI. Finally, we explored the correlation between SIRT1 and ferroptosis via regulating the expression of SIRT1 by resveratrol (RSV) and selisistat (SEL). Our results showed that ferroptosis was involved in the pathogenesis of EBI after SAH through multiple pathways, including acyl-CoA synthetase long-chain family member 4 (ACSL4) activation, iron metabolism disturbance, and the downregulation of glutathione peroxidase 4 (GPX4) and ferroptosis suppressor protein 1 (FSP1). Inhibition of ferroptosis by Fer-1 significantly alleviated oxidative stress-mediated brain injury. SIRT1 activation could suppress SAH-induced ferroptosis by upregulating the expression of GPX4 and FSP1. Therefore, ferroptosis could be a potential therapeutic target for SAH, and SIRT1 activation is a promising method to inhibit ferroptosis.
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http://dx.doi.org/10.1155/2022/9069825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288286PMC
July 2022

Oxytocin Impairs the Recognition of Micro-Expressions of Surprise and Disgust.

Front Psychol 2022 29;13:947418. Epub 2022 Jun 29.

School of Finance and Management, Changsha Social Work College, Changsha, China.

As fleeting facial expressions which reveal the emotion that a person tries to conceal, micro-expressions have great application potentials for fields like security, national defense and medical treatment. However, the physiological basis for the recognition of these facial expressions is poorly understood. In the present research, we utilized a double-blind, placebo-controlled, mixed-model experimental design to investigate the effects of oxytocin on the recognition of micro-expressions in three behavioral studies. Specifically, in Studies 1 and 2, participants were asked to perform a laboratory-based standardized micro-expression recognition task after self-administration of a single dose of intranasal oxytocin (40 IU) or placebo (containing all ingredients except for the neuropeptide). In Study 3, we further examined the effects of oxytocin on the recognition of natural micro-expressions. The results showed that intranasal oxytocin decreased the recognition speed for standardized intense micro-expressions of surprise (Study 1) and decreased the recognition accuracy for standardized subtle micro-expressions of disgust (Study 2). The results of Study 3 further revealed that intranasal oxytocin administration significantly reduced the recognition accuracy for natural micro-expressions of surprise and disgust. The present research is the first to investigate the effects of oxytocin on micro-expression recognition. It suggests that the oxytocin mainly plays an inhibiting role in the recognition of micro-expressions and there are fundamental differences in the neurophysiological basis for the recognition of micro-expressions and macro-expressions.
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http://dx.doi.org/10.3389/fpsyg.2022.947418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277341PMC
June 2022

A potential decision-making algorithm based on endoscopic ultrasound for staging early gastric cancer: a retrospective study.

BMC Cancer 2022 Jul 13;22(1):761. Epub 2022 Jul 13.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Endoscopy, Peking University Cancer Hospital & Institute, #52 Fucheng Road Haidian District, Beijing, China.

Background: Clinical staging of gastric cancer (GC) before treatment is essential. Endoscopic ultrasound (EUS) is a recommended staging tool, but its efficacy remains controversial. Our previous prospective study evaluated the potential value of EUS for T staging and presented discrepancies. In this study, we aimed to evaluate the efficacy of EUS in T staging by comparing it with pathological staging. We analyze the factors that can potentially affect accuracy to identify suitable subgroups for EUS staging.

Methods: Data from a total of 1763 consecutive patients with GC from January 2015 to December 2017 were analyzed. Results from EUS and pathological T staging were compared. The factors that might affect EUS's accuracy were analyzed.

Results: The sensitivity, specificity, positive predictive value, and negative predictive value of EUS in patients with early GC were 62.08%, 96.13%, 90.94%, and 80.21%, respectively. The accuracy rates of uT1, uT2-uT4, and uT3-uT4 were 90.94%, 79.02%, and 78.39%, respectively. In multivariate analysis, underestimation was more likely to be observed in patients with tumors located in the middle or upper third of the stomach. Overestimation was more likely to be observed in patients with tumors located in the lower third or those without ulcer. Other factors affecting accuracy included ulcer, differentiation, larger size and undergoing surgery.

Conclusion: Our findings highlight the role of EUS in determining the T staging of GC. Overestimation and underestimation in T-staging were significantly associated with the tumor location in early GC, and a decision-making algorithm was proposed for clinical practice in early cancers based on these findings.
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http://dx.doi.org/10.1186/s12885-022-09870-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281103PMC
July 2022

Celastrol inhibits TXNIP expression to protect pancreatic β cells in diabetic mice.

Phytomedicine 2022 Sep 5;104:154316. Epub 2022 Jul 5.

Core Facility, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China; Zhejiang Chinese Medical University, Hangzhou 310059, China. Electronic address:

Background: Celastrol (CEL) has a great potential in the treatment of a wide variety of metabolic diseases. However, whether CEL protects pancreatic β cells and its underlying mechanism are not yet clear.

Purpose: This study investigates to determine the effects of CEL on the pathogenesis of pancreatic β cells damage.

Methods: C57BLKS/Lepr (db/db) mice and rat insulinoma INS-1 cell line or mouse J774A.1 cell line were used as in vivo and in vitro models for investigating the protective effect of CEL on pancreatic β cells under high glucose environment and the related mechanism. The phenotypic changes were evaluated by immunofluorescence, immunohistochemical staining, flow cytometry and the measurement of biochemical indexes. The molecular mechanism was explored by biological techniques such as western blotting, qPCR, ChIP-qPCR, co-immunoprecipitation and lentivirus infection.

Results: Our results showed that CEL at the high dose (CEL-H, 0.2 mg/kg) protects db/db mice against increased body weight and blood glucose. CEL-H inhibits pancreatic β cell apoptosis in db/db mice and high glucose-induced INS-1 cells. CEL-H also reduced IL-1β production in islet macrophages. The further study found that CEL suppressed TXNIP expression and NLRP3 inflammasome activation in pancreatic β cells and islet macrophages. Importantly, the inhibitory effect of CEL on pancreatic β cell apoptosis and IL-1β production was also dependent on TXNIP. Mechanically, CEL inhibits Txnip transcription by promoting the degradation of ChREBP.

Conclusion: Celastrol inhibits TXNIP expression to protect pancreatic β cells in vivo and in vitro. Our research pointed out another mechanism by which celastrol functions under the condition leptin signaling is ineffective.
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http://dx.doi.org/10.1016/j.phymed.2022.154316DOI Listing
September 2022

Biomarkers of Metabolomics in Inflammatory Bowel Disease and Damp-Heat Syndrome: A Preliminary Study.

Evid Based Complement Alternat Med 2022 1;2022:3319646. Epub 2022 Jul 1.

Gastroenterology Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.

Aims: This study aims to investigate the potential biomarkers of inflammatory bowel disease (IBD) and IBD with damp-heat syndrome (IBD-DH) by metabolomics.

Methods: Plasma and urine samples were collected from 15 healthy controls and 30 IBD patients, including 15 IBD-DH and 15 IBD with spleen deficiency syndrome (IBD-SD), which was coded as SF8G and SF70 according to the International Classification of Diseases Eleventh Revision (ICD-11) issued by World Health Organization. Pseudotargeted metabolomics method was used based on ultra-high-performance liquid chromatography-high-resolution mass spectrometry and triple-quadrupole mass spectrometry.

Results: Under the condition of false discovery rate (FDR) < 0.05, variable importance projection (VIP) > 1.0, and fold change (FC) > 1.5 or < 2/3, we found 57 plasma differential metabolites and 20 urinary differential metabolites in IBD. Then, with area under the curve (AUC) ≥ 0.85 and FC ≥ 2 or ≤ 0.3, 11 potential biomarkers were identified, such as acylcarnitine (ACar 20:4, ACar 18:1, and ACar 20:3), 3-indoleacetic acid, hippuric acid, and dehydroepiandrosterone sulfate, which is related to intestinal microbiota and immune response. However, less obvious differences were observed in IBD-DH when compared with IBD-SD. Under the condition of FDR < 0.2, VIP >1.0, and FC > 1.5 or < 2/3, we identified 16 plasma differential metabolites. In urine samples, IBD-DH and IBD-SD had the same metabolite pattern. With AUC ≥ 0.80, 7 differential plasma metabolites, mainly glycerophospholipids, were identified in IBD-DH. Kyoto Encyclopedia of Genes and Genomes analysis indicated that metabolic pathways, such as citrate cycle and amino acids metabolism, were mainly responsible for the distinction between IBD and healthy controls, whereas glycerophospholipid metabolism perturbation was not only a manifestation of IBD but also an important pathway to distinguish two subtypes defined by traditional medicine, IBD-DH and IBD-SD.

Conclusion: In this study, we found that several metabolites of aromatic acids and lipid derivatives could act as potential biomarkers to discriminate IBD from healthy controls. Glycerophospholipids metabolites might be used to differentiate IBD-DH from IBD-SD.
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http://dx.doi.org/10.1155/2022/3319646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270137PMC
July 2022

Auxin facilitates cell wall phosphorus reutilization in a nitric oxide-ethylene dependent manner in phosphorus deficient rice (Oryza sativa L.).

Plant Sci 2022 Sep 7;322:111371. Epub 2022 Jul 7.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Auxin is involved in stress responses of plants, such as phosphorus (P) deficiency in rice. Studies on whether auxin participates in cell-wall inorganic phosphorous (Pi) reutilization in Pi-starved rice are scarce. This study explored the mechanisms underlying auxin-facilitated cell-wall Pi-reutilization in rice roots. Pi deficiency rapidly induced auxin accumulation in roots; exogenous auxin [α-naphthaleneacetic acid (NAA), a permeable analog of auxin] elevated soluble Pi content in roots and shoots by increasing pectin content by enhancing activity of pectin methylesterase, and upregulating the transcript level of PHOSPHORUS-TRANSPORTER-2, such that more Pi was translocated to the shoot. Irrespective of the Pi status, exogenous auxin induced nitric oxide (NO) and ethylene production, while exogenous sodium nitroprusside (an NO donor) and 1-aminocyclopropane-1-carboxylic acid (a precursor of ethylene) had no effect on auxin content, suggesting that auxin may act upstream of NO and ethylene. The beneficial effect of NAA in increasing soluble Pi content in roots and shoots disappeared when 2-(4-carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (a scavenger of NO) or aminoethoxyvinylglycine (an inhibitor of ethylene) were applied, suggesting that auxin facilitates cell-wall Pi-reutilization in a NO-ethylene-dependent manner in Pi-deficient rice. Our study results suggest auxin application as an effective agronomic practice for improving plant Pi nutrition in P-deficient conditions.
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http://dx.doi.org/10.1016/j.plantsci.2022.111371DOI Listing
September 2022

Transcriptome and Quasi-Targeted Metabolome Analyze Overexpression of 4-Hydroxyphenylpyruvate Dioxygenase Alleviates Fungal Toxicity of 9-Phenanthrol in .

Int J Mol Sci 2022 Jun 27;23(13). Epub 2022 Jun 27.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.

, the causal agent of rice blast disease, produces devastating damage to global rice production. It is urgent to explore novel strategies to overcome the losses caused by this disease. 9-phenanthrol is often used as a transient receptor potential melastatin 4 (TRPM4) channel inhibitor for animals, but we found its fungal toxicity to . Thus, we explored the antimicrobial mechanism through transcriptome and metabolome analyses. Moreover, we found that overexpression of a gene encoding 4-hydroxyphenylpyruvate dioxygenase involved in the tyrosine degradative pathway enhanced the tolerance of 9-phenanthrol in . Thus, our results highlight the potential fungal toxicity mechanism of 9-phenanthrol at metabolic and transcriptomic levels and identify a gene involving 9-phenanthrol alleviation. Importantly, our results demonstrate the novel mechanism of 9-phenanthrol on fungal toxicity that will provide new insights of 9-phenanthrol for application on other organisms.
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http://dx.doi.org/10.3390/ijms23137116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266922PMC
June 2022

Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats.

Cell Biochem Biophys 2022 Jul 8. Epub 2022 Jul 8.

2nd Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 33 Badachu Road, Shijingshan District, Beijing, 100144, China.

Introduction: Given that denervation atrophy often occurs in muscle after peripheral nerve injury, the effects of injections of human adipose-derived stem cells (hADSCs) and platelet-rich plasma (PRP) into muscle after peripheral nerve injury were examined.

Methods: hADSCs were isolated from human subcutaneous fat tissue, and PRP was prepared from rat whole blood before injection into a rat sciatic nerve injury model. Muscle atrophy was evaluated by quantitating the gross musculature and muscle fiber area and walking footprint analysis.

Results: At 4 weeks post-surgery, there were significant differences in the sciatic functional index between experimental (injected with hADSCs, PRP, or combined hADSCs + PRP) and non-operated groups (p < 0.0001), but no significant differences were observed between the different treatment groups (p > 0.05). Post hoc Bonferroni tests also showed significant differences in the wet muscle weight ratios of hADSC, PRP, and combined groups compared to PBS group. The gastrocnemius muscle fiber area was larger in hADSC group and the combined group compared to PBS group at 4 weeks post-surgery.

Conclusion: The injection of hADSCs delays muscular atrophy after sciatic nerve injury in rats; thus, hADSCs are a promising alternative for regenerating atrophied muscle.
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http://dx.doi.org/10.1007/s12013-022-01082-4DOI Listing
July 2022

[Short needling for knee osteoarthritis with blood stasis obstruction and its effect on serum inflammatory factors].

Zhongguo Zhen Jiu 2022 Jul;42(7):733-8

Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of TCM, Shanghai 200437.

Objective: To compare the clinical efficacy and its effect on serum levels of tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), interleukin 6 (IL-6) and prostaglandin E (PGE) between short needling (close-to-bone needling) and conventional acupuncture for knee osteoarthritis (KOA) with blood stasis obstruction.

Methods: A total of 68 KOA patients with blood stasis obstruction were randomized into a short needling group (34 cases, 3 cases dropped off) and a conventional acupuncture group (34 cases, 3 cases dropped off). The same acupoints (Dubi [ST 35], Neixiyan [EX-LE 4], Binzhong [Extra], Liangqiu [ST 34], etc. on the affected side) were selected in the two groups. In the short needling group, short needling technique was adopted, the needles were slowly inserted and the needle bodies were shaken, thus gradually penetrated to the bone. In the conventional acupuncture group, conventional acupuncture was adopted, the needles were penetrated to the muscle. After -arrival, Dubi (ST 35) and Neixiyan (EX-LE 4), Zusanli (ST 36) and Liangqiu (ST 34) were connected with CMNS6-1 electronic acupuncture instrument, with disperse-dense wave, 2 Hz/10 Hz in frequency, the current intensity was based on patients' feeling, the needles were retained for 30 min, at the same time, the knee joint was irradiated for 30 min with a special electromagnetic wave apparatus in the two groups. Once every other day, 3 times a week for 4 weeks. Before and after treatment, the Western Ontario and McMaster Universities osteoarthritis index (WOMAC) score, knee joint pain visual analogue scale (VAS) score, inflammatory response related indexes (serum TNF-a, IL-1β, IL-6 and PGE) and knee joint ultrasound were observed,and the clinical effect was evaluated in the two groups.

Results: After treatment,the pain, stiffness, function scores and total scores of WOMAC were decreased as compared with those before treatment in the two groups (<0.05), except for the pain score, the changes of above scores in the short needling group were greater than the conventional acupuncture group (<0.05). After treatment, the VAS scores, serum levels of TNF-a, IL-1β, IL-6, PGE and knee joint synovium thickness, intra-articular effusion were decreased as compared with those before treatment in the two groups (<0.05), the levels of TNF-a, IL-1β, IL-6 in the short needling group were lower than the conventional acupuncture group (<0.05). The total effective rate in the short needling group was 87.1% (27/31), which was superior to 83.9% (26/31) in the conventional acupuncture group (<0.05).

Conclusion: Short needling could improve the knee joint function, relieve the pain and inflammatory response, improve the knee joint synovium inflammatory response, reduce the knee joint intra-articular effusion for KOA patients, its effect is better than conventional acupuncture.
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http://dx.doi.org/10.13703/j.0255-2930.20210901-k0008DOI Listing
July 2022

Facial and Neck Reconstruction With Pre-expanded Medial Upper Arm Flap: An Alternative method and 20-Year Experience.

J Craniofac Surg 2022 Jun 29. Epub 2022 Jun 29.

2nd Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Purpose: To present our experience with pre-expanded medial upper arm flap in facial and neck reconstruction.

Patients And Methods: This was a retrospective study operated between January 1st, 2001 and January 1st, 2021, at the Plastic Surgery Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College. Staged face and/or neck reconstruction was performed.

Results: Forty-one patients were treated in our institution and thirty-eight patients (forty-three flaps) were included in this cohort as. They ranged from 6 to 44 years old. There was no total flap loss in the cohort. Partial flap necrosis was observed in the earlier patients (4 cases).

Conclusion: Pre-expanded medial upper arm flap is well matched to the facial and neck skin in color, texture, and thickness. Considering the excellent aesthetic outcomes, this flap is a good alternative for selected patients with soft tissue defects of the head and neck.
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http://dx.doi.org/10.1097/SCS.0000000000008516DOI Listing
June 2022

Strategies to enhance the reactivity of zero-valent iron for environmental remediation: A review.

J Environ Manage 2022 Sep 8;317:115381. Epub 2022 Jun 8.

College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.

Application of zero-valent iron (ZVI) has become one of the most promising innovative technologies for the remediation of environmental pollutants. However, ZVI may suffer from the low intrinsic reactivity toward refractory pollutants, which seriously restricts its practical application in fields. Therefore, strategies have been developing to enhance the reactivity of ZVI. Until now, the most commonly used strategies include pretreatment of ZVI, synthesis of highly-active ZVI-based materials and additional auxiliary measures. In this review, a systematic and comprehensive summary of these commonly used strategies has been conducted for the following purposes: (1) to understand the fundamental mechanisms of the selected approaches; (2) to point out their advantages and shortcomings; (3) to illustrate the main problems of their large-scale application; (4) to forecast the future trend of developing ZVI technologies. Overall, this review is devoted to providing a fundamental understanding on the mechanism for enhancing the reactivity of ZVI and facilitating the practical application of ZVI technologies in fields.
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http://dx.doi.org/10.1016/j.jenvman.2022.115381DOI Listing
September 2022

Vertically growing nanowall-like N-doped NiP/NF electrocatalysts for the oxygen evolution reaction.

Dalton Trans 2022 Jul 5;51(26):10160-10168. Epub 2022 Jul 5.

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, China.

Developing low-cost, high-performance and corrosion-resistant catalysts for water splitting is anticipated, but it will also be a big challenge. In this study, nanowall-like N-NiP/NiP/NF (N-NiP/NF) was synthesized by a simple two-step method involving hydrothermal treatment and phosphorylation. The catalyst has good catalytic activity for the OER, and only 160 mV is required to achieve a current density of 10 mA cm in 1 M KOH, which is even better than RuO, with good corrosion resistance. In addition, N-CoP/NiP/NF (N-CoP/NF) was synthesized by the same method with good electrocatalytic properties and good conductivity towards the HER. N-NiP/NF was used as the anode and N-CoP/NF was used as the cathode to form the N-NiP//N-CoP double electrode system, which showed excellent electrolytic performance for water splitting, requiring only 1.48 V to reach 10 mA cm. This is mainly due to the strong electronegativity of N that makes the N doping induce the electron transfer process, which results in a high catalytic activity of the adjacent transition metal atoms and thus promotes the electrolysis of water, as well as the unique vertical nanowall-like structure, which gives the material a large surface area and accessibility to active sites, facilitating the adsorption of water molecules and catalytic reactions. In addition, the unique structure favors the diffusion of water molecules and the release of gaseous products, ensuring close contact between the catalyst and the electroactive material. This simple non-metallic N doping strategy provides a new way to produce efficient non-precious metal catalysts.
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http://dx.doi.org/10.1039/d2dt01494gDOI Listing
July 2022

Iron deficiency exacerbates aortic medial degeneration by inducing excessive mitochondrial fission.

Food Funct 2022 Jul 18;13(14):7666-7683. Epub 2022 Jul 18.

Department of Cardiothoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, People's Republic of China.

Iron deficiency (ID) is a global nutritional deficiency that was shown to be involved in the pathogenesis of aortic aneurysm and dissection (AAD) in our previous studies. Some studies suggested that mitochondrial dynamics was involved in the apoptosis and phenotypic transformation of vascular smooth muscle cells (VSMCs). However, little is known about the role of mitochondrial dynamics in aortic medial degeneration (AMD) promoted by an iron deficient diet. The present study investigated the effect of ID on the phenotypic transformation of VSMCs, the progression of AMD, and the underlying mechanism. The expression of p-Drp1 (Ser616) and Fis1 was markedly upregulated in the aortic media of AAD patients and ApoE mice with subcutaneous AngII osmotic pumps. ID facilitated the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs), which triggered excessive mitochondrial fission, induced the phenotypic transformation of VSMCs, and ultimately accelerated the progression of AMD. Furthermore, the present study indicated that an inhibitor of Drp1 could partially reverse this process. Maintaining iron balance in the human body may prevent the development of AAD.
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http://dx.doi.org/10.1039/d2fo01084dDOI Listing
July 2022

A Hybrid Functional Study on Perovskite-Based Compounds CsPbZnIX (X = Cl or Br).

J Phys Chem Lett 2022 Jun 21;13(25):5900-5909. Epub 2022 Jun 21.

School of Physical Science and Technology, Chongqing Key Laboratory of Micro & Nano Structure Optoelectronics, Southwest University, Chongqing 400715, People's Republic of China.

Inorganic perovskites have attracted a great deal of attention because of their stability. Unfortunately, a weak optical response and the toxicity of lead are hampering their development. Motivated by these facts, we focus herein on the perovskite-based doped series CsPbZnIX (X = Cl or Br). The geometric structures and the electronic and optical properties of CsPbZnIX (X = Cl or Br) are investigated systematically by hybrid functional theory. Analysis of the electronic properties indicates that Zn/Cl/Br mono-doping and co-doping efficiently tune bandgaps. Moreover, we find that the ability to obtain electrons for CsPbZnICl is superior to the abilities of the others, which implies a stronger electron transition. In addition, CsPbZnICl and CsPbZnIBr show stronger visible-light responses in the range of 467-780 nm. Both CsPbZnICl and CsPbZnIBr are hence good choices for photovoltaic applications. Furthermore, the physically accessible region is also explored herein. These findings shed new light on the design of highly efficient and low-lead perovskite-based optoelectronic materials.
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http://dx.doi.org/10.1021/acs.jpclett.2c01239DOI Listing
June 2022
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