Publications by authors named "Qi Wang"

5,015 Publications

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Airborne microplastics in indoor and outdoor environments of a coastal city in Eastern China.

J Hazard Mater 2021 May 6;417:126007. Epub 2021 May 6.

Key Laboratory of Watershed Sciences and Health of Zhejiang Province, School of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China. Electronic address:

Microplastics (MPs) in marine and terrestrial environments have been intensively studied, but the dynamics of airborne MPs remains limited. Existing studies on atmospheric MPs are mostly derived from collection of atmospheric deposition, whereas direct measurements of airborne MPs are scarce. However, the abundance of airborne MPs is more relevant for evaluating human inhalation exposure risk. Herein, airborne MPs in indoor and outdoor environments from urban and rural areas of a coastal city in eastern China were investigated. MP concentrations (mean±SD) in indoor air (1583 ± 1180 n/m) were an order of magnitude higher than outdoor air (189 ± 85 n/m), and airborne MP concentrations in urban areas (224 ± 70 n/m) were higher than rural areas (101 ± 47 n/m). MPs smaller than 100 µm dominated airborne MPs, and the predominant shape of airborne MPs was fragments, as opposed to fibers. The larger MP size fractions contained a higher proportion of fibers, whereas the smaller size fractions were nearly exclusively composed of fragments. The health risk caused by ubiquitous airborne MPs should not be discounted as the maximum annual outdoor exposure of airborne MPs can reach 1 million/year, while indoor exposure may be even higher due to higher indoor airborne MP concentrations.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126007DOI Listing
May 2021

Design, synthesis, in vitro and in vivo evaluation against MRSA and molecular docking studies of novel pleuromutilin derivatives bearing 1, 3, 4-oxadiazole linker.

Bioorg Chem 2021 May 1;112:104956. Epub 2021 May 1.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. Electronic address:

A class of pleuromutilin derivatives containing 1, 3, 4-oxadiazole were designed and synthesized as potential antibacterial agents against Methicillin-resistant staphylococcus aureus (MRSA). The ultrasound-assisted reaction was proposed as a green chemistry method to synthesize 1, 3, 4-oxadiazole derivatives (intermediates 85-110). Among these pleuromutilin derivatives, compound 133 was found to be the strongest antibacterial derivative against MRSA (MIC = 0.125 μg/mL). Furthermore, the result of the time-kill curves displayed that compound 133 could inhibit the growth of MRSA in vitro quickly (- 4.36 log10 CFU/mL reduction). Then, compound 133 (- 1.82 log CFU/mL) displayed superior in vivo antibacterial efficacy than tiamulin (- 0.82 log CFU/mL) in reducing MRSA load in mice thigh model. Besides, compound 133 exhibited low cytotoxicity to RAW 264.7 cells. Molecular docking studies revealed that compound 133 was successfully localized in the binding pocket of 50S ribosomal subunit (ΔG = -10.50 kcal/mol). The results indicated that these pleuromutilin derivatives containing 1, 3, 4-oxadiazole might be further developed into novel antibiotics against MRSA.
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http://dx.doi.org/10.1016/j.bioorg.2021.104956DOI Listing
May 2021

Research on innovation management of science and technology innovation team members based on constructive conflict evolution game.

J Investig Med 2021 May 14. Epub 2021 May 14.

School of Commerce, Waseda University, Shinjuku-ku, Tokyo, Japan.

How to give full play to the positive function of constructive conflicts (task conflicts and process conflicts) in the science and technology innovation team, give appropriate stimulation, mobilize the enthusiasm of employees and improve the team's innovative ability to improve team performance and organizational effectiveness are issues that deserve both team leaders' and scholars' attention. Through selecting multiple medical technology R&D personnel from a specific science and technology innovation team of health organization, the research studies the constructive conflicts among the members of the science and technology innovation team, constructs and analyzes the conflict evolution game model, proposes countermeasures and suggestions for improving the innovation ability of the science and technology innovation team and discusses the innovation management mechanism of the science and technology innovation team. The study shows that task conflicts, process conflicts and innovation game decisions cannot be avoided. The unstable choice of members does not promote innovation. However, on the one hand, constructive conflicts can be controlled to maintain a moderate state of control. On the other hand, it is also necessary to establish a mutually trusting communication environment and convenient communication channels in the science and technology innovation team, combined with modern information management technology, to handle the problems that were difficult to be found or accumulated for a long time under the previous management mechanism and cooperate with the science and technology innovation team to improve the technology innovation team's innovation capability.
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http://dx.doi.org/10.1136/jim-2020-001676DOI Listing
May 2021

A novel visible light-driven TiO photocatalytic reduction for hexavalent chromium wastewater and mechanism.

Water Sci Technol 2021 May;83(9):2135-2145

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China E-mail:

Titanium dioxide (TiO) photocatalyst was prepared with a sol-gel method and its characterizations were analyzed TiO photocatalytic reduction of Cr was investigated in visible light irradiation and reduction mechanisms were calculated. Prepared TiO is anatase with a bandgap of about 2.95 eV. Experimental results display that almost 100% of Cr is removed by visible light-driven TiO photocatalytic reduction after 120 min when CrO initial concentration is 1.0 mg·L, TiO dosage is 1.0 g·L, and pH value is 3. In acidic aqueous solution, HCrO is the dominant existing form of Cr and is adsorbed by TiO, forming a complex catalyst HCrO/TiO with an increase in wavelength to the visible light zone, demonstrated by UV-Vis diffuse reflection spectroscopy. Based on X-ray photoelectron spectroscopy data, it can be deduced that Cr is adsorbed on the surface of TiO and then reduced to Cr in situ by photoelectrons. Self-assembly of HCrO/TiO complex catalyst and self-reduction of Cr in situ are the key steps to start the visible light-driven TiO photocatalytic reduction. Furthermore, TiO photocatalytic reduction of Cr fits well with pseudo-first-order kinetics and has the potential application to treat chemical industrial wastewater.
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http://dx.doi.org/10.2166/wst.2021.116DOI Listing
May 2021

Lycopene alleviates hepatic ischemia reperfusion injury via the Nrf2/HO-1 pathway mediated NLRP3 inflammasome inhibition in Kupffer cells.

Ann Transl Med 2021 Apr;9(8):631

School of Medicine, Southeast University, Nanjing, China.

Background: Lycopene is a naturally occurring carotenoid found in many fruits and vegetables, which has antioxidant effects. Although lycopene's protective effect has been observed on ischemia reperfusion (IR) injury in different organs, the effect of lycopene on Kupffer cells (KCs) has not been clearly elucidated in IR-induced acute hepatic inflammatory injury.

Methods: Mice were administered with either olive oil (10 mL/kg body weight) as the control or lycopene (20 mg/kg body weight) by gavage for 2 weeks before undergoing hepatic IR injury.

Results: In this study, we observed that the levels of aspartate aminotransferases (AST), alanine aminotransferase (ALT), and the percentages of hepatocellular apoptosis in mice pretreated with lycopene were significantly lower than control mice. Lycopene inhibited F4/80+ macrophage and Ly6G+ neutrophil accumulation, which further decreased the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin 6 (IL-6). Interestingly, lycopene induced increased autophagy in KCs, which was evidenced by elevated autophagosomes and the increased protein level of LC3B. In these KCs, lycopene-induced upregulation of autophagy inhibited NOD-like receptor family pyrin domain-containing 3 protein (NLRP3) inflammasome activation, which was demonstrated by the reduced mRNA and protein levels of NLRP3, cleaved caspase-1, an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and IL-1β. Furthermore, 3-methyladenine, an autophagy inhibitor, abolished lycopene's inhibitory effect on the NLRP3 inflammasome in KCs, which led to increased hepatic IR injury. Intriguingly, we identified that the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) were elevated in KCs isolated from IR-stressed mice pretreated with lycopene. Nrf2-siRNA or HO-1-siRNA could block the autophagy activation enhanced by lycopene in KCs, resulting in the activation of the NLRP3 inflammasome and aggravated hepatic IR injury.

Conclusions: Our findings demonstrated that lycopene promoted Nrf2/HO-1 pathway activation and further suppressed the NLRP3 inflammasome via enhancing KC autophagy, which alleviated hepatic IR injury.
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http://dx.doi.org/10.21037/atm-20-7084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106004PMC
April 2021

Identification of four genes and biological characteristics associated with acute spinal cord injury in rats integrated bioinformatics analysis.

Ann Transl Med 2021 Apr;9(7):570

Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.

Background: Spinal cord injury (SCI) is a serious condition that can cause physical disability and sensory dysfunction. Cytokines play an extremely important role in the acute phase of SCI. Clarifying the cytokine expression profile is of great importance.

Methods: Cytokine array analysis was used to explore the changes in 67 different proteins at 0 hours, 2 hours, 1 day, 3 days, and 7 days after acute SCI in rats. The differentially expressed cytokines in the various periods were analyzed and compared. The biological processes related to the differentially expressed proteins were examined using Gene Ontology (GO) analysis.

Results: Immediately after SCI (0 hours), only ciliary neurotrophic factor (CNTF) was slightly up-regulated, while 23 other proteins were down-regulated. At 2 hours after SCI, there were 3 upregulated and 21 downregulated proteins. At 1 day after SCI, there were 5 upregulated and 6 downregulated proteins. At 3 days after SCI, there were 6 upregulated and 4 downregulated proteins. At 7 days after SCI, there were 4 upregulated and 9 downregulated proteins. Erythropoietin (EPO) and Fms related tyrosine kinase 3 ligand (Flt-3L) were downregulated at all time points. CD48 was decreased at 2 hours to 7 days after SCI. Monocyte chemotactic protein-1 (MCP-1) was the only protein that was upregulated at 2 hours to 7 days. The GO and pathway analyses revealed that the cytokine-related pathways, cell death, and proliferation might play a key role during secondary SCI.

Conclusions: This study identified 3 downregulated proteins during SCI, that being EPO, Flt-3L, and CD48. MCP-1 was the only upregulated protein, and its expression was upregulated till day 7 following SCI. These 4 identified genes may be potential therapeutic targets for the treatment of SCI.
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http://dx.doi.org/10.21037/atm-21-603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105796PMC
April 2021

Inhibition of bone morphogenetic protein receptor 2 suppresses pancreatic ductal adenocarcinoma growth by regulating GRB2/PI3K/AKT axis.

Ann Transl Med 2021 Apr;9(7):557

Department of General Surgery, Peking University First Hospital, Beijing, China.

Background: Bone morphogenetic protein receptor 2 (BMPR2) is an important transmembrane serine/threonine kinase that involves oncogenic processes in multiple cancers. However, the role of BMPR2 and its regulatory mechanism in pancreatic ductal adenocarcinoma (PDAC) remain unknown.

Methods: We performed a tissue array to explore the expression of BMPR2 in PDAC tissues. The Cell Counting Kit-8 (CCK-8) and colony formation assays were used to measure PDAC cells' proliferation. Proteomics and mass spectrometry technology was applied to analyze the BMPR2-regulating proteins. Flow cytometry was used to analyze the cell cycle distribution of PDAC cells. Orthotopic pancreatic cancer (PC) and patient-derived xenograft (PDX) models were used for experiments.

Results: This study revealed the over-expression of BMPR2 in PDAC tissues and its proliferation-promoting role in PDAC cells. By carrying out protein mass spectrometry technique as well as bioinformatics analysis, we identified that BMPR2 regulated the growth factor receptor-bound protein 2/phosphatidylinositol 3-kinase/protein kinase B (GRB2/PI3K/AKT) signaling pathway, and further in vitro experiments showed that inhibition of BMPR2 resulted in suppressing proliferation and G2/M arrest by inhibiting the GRB2/PI3K/AKT signaling pathway in PDAC cells. The inhibition of BMPR2 by LDN193189 showed similar results in PDAC cells, orthotopic PC, and PDX models, which revealed that inhibition of BMPR2 significantly suppressed tumor growth by suppressing the GRB2/PI3K/AKT axis.

Conclusions: Inhibition of BMPR2 suppresses PDAC growth by regulating the GRB2/PI3K/AKT axis and is a promising PDAC treatment strategy.
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http://dx.doi.org/10.21037/atm-20-2194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105856PMC
April 2021

Bioinformatics and Expression Analysis of IDA-Like Genes Reveal Their Potential Functions in Flower Abscission and Stress Response in Tobacco ( L.).

Front Genet 2021 27;12:670794. Epub 2021 Apr 27.

Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.

The inflorescence deficient in abscission-like () genes have been shown to play critical roles in floral organ abscission, lateral root formation and various stress responses in . The gene family has been characterized in a number of plant species, while limited information is available about genes of tobacco. In the current study, 15 NtIDL members were identified in the tobacco genome, and were classified into six groups together with IDL members from other species. Evolution analysis suggested that the NtIDL members form group VI might have originated from duplication events. Notably, NtIDL06 shared high similarities with AtIDA in the EPIP sequence, and its encoding gene was highly expressed in the abscission zone of flowers at late developmental stages, implying that NtIDL06 might regulate tobacco flower abscission. In addition, the results from -elements analysis of promoters and expression after stress treatments suggested that members might be involved in various stress responses of tobacco. The results from this study provide information for further functional analysis related to flower abscission and stress responses of genes.
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http://dx.doi.org/10.3389/fgene.2021.670794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110903PMC
April 2021

Corrigendum to "Effect of aromatherapy on preoperative anxiety in adult patients: A meta-analysis of randomized controlled trials" [Compl. Ther. Clin. Pract. 42 (February 2021) 101302].

Complement Ther Clin Pract 2021 May 10:101411. Epub 2021 May 10.

Department of Psychiatry, The First Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:

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http://dx.doi.org/10.1016/j.ctcp.2021.101411DOI Listing
May 2021

Dielectric barrier discharge plasma-assisted modification of g-CN/AgO/TiO-NRs composite enhanced photoelectrocatalytic activity.

J Environ Sci (China) 2021 Jun 18;104:113-127. Epub 2020 Dec 18.

School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, China. Electronic address:

Dielectric barrier discharge (DBD) plasma applied as surface treatment technology was employed for the modification of AgO and graphitic carbon nitride (g-CN) powders. Subsequently, the pretreated powders were sequentially loaded onto TiO nanorods (TiO-NRs) via electro-deposition, followed by calcination at N atmosphere. The results indicated that at the optimal plasma discharge time of 5 min for modification of g-CN and AgO, photocurrent density of ternary composite was 6 times to bare TiO-NRs under UV-visible light irradiation. Phenol was degraded by using DBD plasma-modified g-CN/AgO/TiO-NRs electrode to analyze the photoelectrocatalytic performance. The removal rate of phenol for g-CN-5/AgO-5/TiO-NRs electrode was about 3.07 times to that for TiO-NRs electrode. During active species scavengers' analysis, superoxide radicals and hydroxyl radicals were the main oxidation active species for pollutants degradation. A possible electron-hole separation and transfer mechanism of ternary composite with high photoelectrocatalytic performance was proposed.
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http://dx.doi.org/10.1016/j.jes.2020.11.029DOI Listing
June 2021

Investigation on many-body effects in micro-LEDs under ultra-high injection levels.

Opt Express 2021 Apr;29(9):13219-13230

Micro-LEDs can work under an extremely high injection level and are widely used in high-brightness micro-displays and visible light communication. With the increase of carrier concentration, many-body effects gradually become important factors affecting devices' characteristics. Considering the effects of carrier scattering, bandgap renormalization, and Coulomb enhancement (CE), changes in the electroluminescence spectra of micro-LEDs are analyzed as the current density increases from 49.2 to 358.2 kA/cm, the latter representing an ultra-high injection level. Affected by plasma screening, CE decreases below about 150 kA/cm. After that, polarization screening dominates and effectively alleviates the spatial separation of electrons and holes, which results in CE increases to the maximum injection level of 358.2 kA/cm. It is established that CE promotes radiative recombination processes. Different from the traditional phenomenon of "efficiency droop", the enhanced attraction between carriers leads to an abnormal increase of external quantum efficiency at high current density.
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http://dx.doi.org/10.1364/OE.422151DOI Listing
April 2021

DNA Methylation and Expression Profiles of Whole Blood in Parkinson's Disease.

Front Genet 2021 26;12:640266. Epub 2021 Apr 26.

Neurodegenerative Disease Research Center, Biodesign Institute, Arizona State University, Tempe, AZ, United States.

Parkinson's disease (PD) is the second most common age-related neurodegenerative disease. It is presently only accurately diagnosed at an advanced stage by a series of motor deficits, which are predated by a litany of non-motor symptoms manifesting over years or decades. Aberrant epigenetic modifications exist across a range of diseases and are non-invasively detectable in blood as potential markers of disease. We performed comparative analyses of the methylome and transcriptome in blood from PD patients and matched controls. Our aim was to characterize DNA methylation and gene expression patterns in whole blood from PD patients as a foundational step toward the future goal of identifying molecular markers that could predict, accurately diagnose, or track the progression of PD. We found that differentially expressed genes (DEGs) were involved in the processes of transcription and mitochondrial function and that PD methylation profiles were readily distinguishable from healthy controls, even in whole-blood DNA samples. Differentially methylated regions (DMRs) were functionally varied, including near transcription factor nuclear transcription factor Y subunit alpha (), receptor tyrosine kinase , RING finger ubiquitin ligase (), acetyltransferase , and vault RNA . Expression quantitative trait methylation sites were found at long non-coding RNA and transcription regulator among others. Functional epigenetic modules were highlighted by , , and . We identified patterns of altered disease-specific DNA methylation and associated gene expression in whole blood. Our combined analyses extended what we learned from the DEG or DMR results alone. These studies provide a foundation to support the characterization of larger sample cohorts, with the goal of building a thorough, accurate, and non-invasive molecular PD biomarker.
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http://dx.doi.org/10.3389/fgene.2021.640266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107387PMC
April 2021

Kinetics, pathways and toxicity of hexabromocyclododecane biodegradation: Isolation of the novel bacterium Citrobacter sp. Y3.

Chemosphere 2021 Jul 15;274:129929. Epub 2021 Feb 15.

School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China.

This research investigated the biodegradation kinetics, pathways and ecological risk of hexabromocyclododecane (HBCD) by a novel bacterium Citrobacter sp. Y3. Results showed the biodegradation followed a first-order model. The specific degradation rate constant of HBCD were obviously higher in batch experiments with combined carbon sources (k: 0.156-0.290 d) than those using HBCD as the sole carbon source (k: 0.055 d). Correspondingly, the degradation half-life became much shorter (T: 2.39-4.44 d vs T: 13.7 d). HBCD could be degraded through dehydrobromination and dehalohydroxylation, of which six possible degradation products were detected. To evaluate the ecological risk of HBCD biodegradation products, acute toxicity tests were assessed for the first time. The acute toxicity decreased slowly during treatment for 3-5 d and then decreased sharply. In general, treatment by Strain Y3 is not only a biodegradation process but also a detoxification process, thus it shows potential for bioremediation of HBCD contaminated sites.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129929DOI Listing
July 2021

Inverse-design magnonic devices.

Nat Commun 2021 May 11;12(1):2636. Epub 2021 May 11.

Fachbereich Physik and Landesforschungszentrum OPTIMAS, Technische Universität Kaiserslautern, Kaiserslautern, Germany.

The field of magnonics offers a new type of low-power information processing, in which magnons, the quanta of spin waves, carry and process data instead of electrons. Many magnonic devices were demonstrated recently, but the development of each of them requires specialized investigations and, usually, one device design is suitable for one function only. Here, we introduce the method of inverse-design magnonics, in which any functionality can be specified first, and a feedback-based computational algorithm is used to obtain the device design. We validate this method using the means of micromagnetic simulations. Our proof-of-concept prototype is based on a rectangular ferromagnetic area that can be patterned using square-shaped voids. To demonstrate the universality of this approach, we explore linear, nonlinear and nonreciprocal magnonic functionalities and use the same algorithm to create a magnonic (de-)multiplexer, a nonlinear switch and a circulator. Thus, inverse-design magnonics can be used to develop highly efficient rf applications as well as Boolean and neuromorphic computing building blocks.
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http://dx.doi.org/10.1038/s41467-021-22897-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113576PMC
May 2021

pH-sensitive thiamethoxam nanoparticles based on bimodal mesoporous silica for improving insecticidal efficiency.

R Soc Open Sci 2021 Feb 17;8(2):201967. Epub 2021 Feb 17.

Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, People's Republic of China.

In this study, we synthesized pH-sensitive thiamethoxam-3-(2-aminoethylamino) propyl-bimodal mesoporous silica (P/Thi-NN-BMMs) nanoparticles (NPs). We used this bimodal mesoporous silica (BMMs) mesoporous material as a carrier based on the principle of free radical polymerization. The size of the P/Thi-NN-BMMs NPs was about 891.7 ± 4.9 nm, with a zeta potential of about -25.7 ± 2.5 mV. X-ray powder diffraction analysis, N-sorption measurements and thermogravimetric analysis indicated that thiamethoxam (Thi) was loaded into the pores of the mesoporous structure and that the mesopore surface was coated with polyacrylic acid (PAA). The loading rate of P/Thi-NN-BMMs was about 25.2%. The controlled-release NPs had excellent anti-photolysis performance and storage stability. The NPs showed significant pH sensitivity, and the Thi release rate in pH 10.0 phosphate buffer was higher than those in pH 7.4 and pH 3.0 phosphate buffers. We described the sustained-release curves according to the Weibull model. The relative toxicity of P/Thi-NN-BMMs against peach aphid was 1.44 times that of commercial Thi. This provides a promising instrument for effective insect control and environment protection.
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http://dx.doi.org/10.1098/rsos.201967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074711PMC
February 2021

Prevalence of coccidia in domestic pigs in China between 1980 and 2019: a systematic review and meta-analysis.

Parasit Vectors 2021 May 10;14(1):248. Epub 2021 May 10.

College of Life Science, Changchun Sci-Tech University, Shuangyang, 130600, Jilin, People's Republic of China.

Background: Swine coccidiosis, a protozoan disease caused by coccidia, can result in diarrhoea and weight loss in piglets and even economic losses in the pig industry. Here, we report the first systematic review and meta-analysis of the prevalence of coccidia (including Eimeria spp. and Cystoisospora suis) in pigs in China.

Methods: Five databases (PubMed, ScienceDirect, Chinese Web of Knowledge, Wanfang, and Chongqing VIP) were searched and 50 studies (46,926 domestic pigs, 22 provinces) ultimately identified pertaining to the prevalence of coccidia infection from 1980 to 2019. We incorporated the effect size using the random-effects model in the "meta" package in R software and conducted univariate and multivariate meta-regression analyses using a mixed-effects model.

Results: The pooled prevalence rate of coccidia in pigs was 21.9%, including the C. suis infection rate of 9.1%. The highest prevalence of coccidia (39.6%) was found in northwest China, and this region also presented the lowest prevalence of C. suis (4.7%). In the subgroup analysis based on sampling year, the highest prevalence of coccidia was detected in 2001 or earlier (32.6%), whereas the lowest rate was found in 2012 or later (14.3%). An opposite trend was observed for C. suis (5.5% in 2000 or earlier vs 14.4% in 2000 or later). The prevalence of coccidia in extensive farming systems (29.5%) was higher than that in intensive farming systems (17.3%). In contrast, the point estimate of C. suis prevalence was lower in the extensive farming systems (5.1%) than in the intensive farming systems (10.0%), but the difference was not significant (P > 0.05). Among the four age categories, the highest total coccidia prevalence (26.2%) was found in finishing pigs, followed by suckling piglets (19.9%), whereas the highest prevalence of C. suis (14.9%) was observed in suckling piglets.

Conclusions: Our findings suggest that coccidia infection in Chinese pigs is common, although the prevalence of C. suis in pigs does not receive sufficient attention. We recommend the rational use of anticoccidial drugs to avoid drug resistance and the development of preventive and control measures for C. suis to reduce the incidence of swine coccidiosis.
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http://dx.doi.org/10.1186/s13071-021-04611-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108339PMC
May 2021

miRNA-193a-3p Regulates the AKT2 Pathway to Inhibit the Growth and Promote the Apoptosis of Glioma Cells by Targeting ALKBH5.

Front Oncol 2021 23;11:600451. Epub 2021 Apr 23.

Department of Neurosurgery, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Emerging evidence indicates that microRNA (miR)-193a-3p is involved in the tumor progression of various cancers. However, the biological functions and precise molecular mechanisms of miR-193a-3p in gliomas have not been well documented. Accordingly, this study focused on the tumor suppressor role and molecular mechanisms of miR-193a-3p in glioma cells. miR-193a-3p expression was determined by qRT-PCR in glioma tissues and cell lines. U251 and U87 glioma cells were transfected with a miR-193a-3p mimic. The effects of miR-193a-3p on cell growth and apoptosis were investigated using MTT, colony-forming, and flow cytometry assays. Overexpression of miR-193a-3p in U87 cells also significantly suppressed tumorigenicity and induced apoptosis in the xenograft mouse model. Luciferase assays were conducted to determine if ALKBH5 is a direct target of miR-193a-3p in glioma cells. Immunoprecipitation was used to explore the interaction between ALKBH5 and RAC-serine/threonine-protein kinase 2 (AKT2) in glioma cells. miR-193a-3p was downregulated in glioma tissues and cell lines. miR-193a-3p treatment suppressed proliferation and promoted apoptosis in both U251 and U87 cells. Bioinformatics analysis and luciferase reporter assay identified a novel miR-193a-3p target, ALKBH5. Notably, the antitumor effect of miR-193a-3p transfection in glioma cells may be due to the miR-193a-3p-induced inhibition of AKT2 expression caused by the suppression of ALKBH5 expression. Furthermore, immunoprecipitation indicated that ALKBH5 physically interacted with AKT2 through an RNA-independent mechanism in glioma cells. miR-193a-3p directly targets ALKBH5 to inhibit the growth and promote the apoptosis of glioma cells by suppressing the AKT2 pathway both and , and the physical interaction between ALKBH5 and AKT2 is essential for suppressing cell apoptosis by upregulating miR-193a-3p in glioma cells. Our study revealed that the antitumor effects of miR-193a-3p on glioma cells is due to ALKBH5 mediation of the AKT2-induced intrinsic apoptosis signaling pathway.
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http://dx.doi.org/10.3389/fonc.2021.600451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103841PMC
April 2021

Corrigendum: Impact of Divergent Thinking Training on Teenagers' Emotion and Self-Efficacy During the COVID-19 Pandemic.

Front Psychol 2021 19;12:686118. Epub 2021 Apr 19.

Department of Psychology, Center for Studies of Social Psychology, Central China Normal University, Wuhan, China.

[This corrects the article DOI: 10.3389/fpsyg.2021.600533.].
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http://dx.doi.org/10.3389/fpsyg.2021.686118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098659PMC
April 2021

CCR7-CCL21 axis promotes the cervical lymph node metastasis of tongue squamous cell carcinoma by up-regulating MUC1.

J Craniomaxillofac Surg 2021 Apr 20. Epub 2021 Apr 20.

Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, The Affiliated Stomatological Hospital of Nankai University, Tianjin, 300041, China; Department of Oral and Maxillofacial Surgery, Tianjin Stomatological Hospital, The Affiliated Stomatological Hospital of Nankai University, Tianjin, 300041, China; Tianjin Key Laboratory of Lung Regenerative Medicine, Tianjin Haihe Hospital, Tianjin, 300350, China. Electronic address:

This study aims at investigating the potential role of MUC1 in CCR7-CCL21 axis-induced metastasis of tongue squamous cell carcinoma (TSCC). TSCC patients were selected for epidemiologic trends. The expression of CCR7 and MUC1 was detected via immunohistochemistry. SCC15 and CAL27 cells were induced by CCL21 and specific antibody to CCR7. Gene and protein expression was detected using qRT-PCR and western blotting. Migration and invasion capacities of TSCC cells were determined using wound healing and Transwell invasion assays. The male:female ratio of 78 patients was 1.6:1. Metastasis rate of cervical lymph nodes (CLNs) was 42.3%. CLN metastasis significantly correlated with T staging (P = 0.026), clinical staging (P = 0.024), and depth of invasion (DOI, P = 0.001). DOI significantly influenced CLN metastasis (P = 0.033, OR = 10.919) of TSCC, as did CCR7 (P = 0.041) and MUC1 (P = 0.026). The consistency of CCR7 and MUC1 expression was fairly good (Kappa = 0.683, P < 0.001). Reduced survival was significantly associated with higher expression of CCR7 (P = 0.039) and MUC1 (P = 0.030). CCL21 up-regulated MUC1 in SCC15 cells, which was inhibited when CCR7 was blocked. MUC1 positively correlated with TSCC cell migration and invasion. CCR7-CCL21 axis might promote CLN metastasis of TSCC by up-regulating MUC1. CCR7 and MUC1 show promise as potential biomarkers for TSCC treatment.
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http://dx.doi.org/10.1016/j.jcms.2021.02.027DOI Listing
April 2021

Simulation Study of Breast Cancer Lipid Changes Affecting Membrane Oxygen Permeability: Effects of Chain Length and Cholesterol.

Adv Exp Med Biol 2021 ;1269:15-21

Department of Chemistry, New Mexico Institute of Mining and Technology (New Mexico Tech), Socorro, NM, USA.

Tumor radiotherapy relies on intracellular oxygen (O) to generate reactive species that trigger cell death, yet hypoxia is common in cancers of the breast. De novo lipid synthesis in tumors supports cell proliferation but also may lead to unusually high levels of the 16:1 palmitoleoyl (Y) phospholipid tail, which is two carbons shorter than the 18:1 oleoyl (O) tail abundant in normal breast tissue. Here, we use atomic resolution molecular dynamics simulations to test two hypotheses: (1) the shorter, 16:1 Y, tail of the de novo lipid biosynthesis product 1-palmitoyl,2-palmitoleoyl-phosphatidylcholine (PYPC) promotes lower membrane permeability relative to the more common lipid 1-palmitoyl,2-oleoylphosphatidylcholine (POPC), by reducing oxygen solubility in the interleaflet region, and (2) cholesterol further lessens the permeability of PYPC by reducing overall O solubility and promoting PYPC tail order adjacent to the rigid cholesterol ring system. The simulations conducted here indicate that PYPC has a permeability of 14 ± 1 cm/s at 37 °C, comparable to 15.4 ± 0.4 cm/s for POPC. Inclusion of cholesterol in a 1:1 ratio with phospholipid intensifies the effect of chain length, giving permeabilities of 10.2 ± 0.2 cm/s for PYPC/cholesterol and 11.0 ± 0.6 cm/s for POPC/cholesterol. These findings indicate that PYPC may not substantially influence membrane-level oxygen flux and is unlikely to hinder breast tissue oxygenation.
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http://dx.doi.org/10.1007/978-3-030-48238-1_3DOI Listing
May 2021

Identification of pseudolaric acid B as a novel Hedgehog pathway inhibitor in medulloblastoma.

Biochem Pharmacol 2021 May 5:114593. Epub 2021 May 5.

Institute of Clinical Pharmacology, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Research Center of Chinese Herbal Resources Science and Engineering, School of Pharmaceutical Sciences, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address:

Aberrant activation of the Hedgehog (Hh) pathway is implicated in the pathogenesis and development of multiple cancers, especially Hh-driven medulloblastoma (MB). Smoothened (SMO) is a promising therapeutic target of the Hh pathway in clinical cancer treatment. However, SMO mutations frequently occur, which leads to drug resistance and tumor relapse. Novel inhibitors that target both the wild-type and mutant SMO are in high demand. In this study, we identified a novel Hh pathway inhibitor, pseudolaric acid B (PAB), which significantly inhibited the expression of Gli1 and its transcriptional target genes, such as cyclin D1 and N-myc, thus inhibiting the proliferation of DAOY and Ptch1 primary MB cells. Mechanistically, PAB can potentially bind to the extracellular entrance of the heptahelical transmembrane domain (TMD) of SMO, based on molecular docking and the BODIPY-cyclopamine binding assay. Further, PAB also efficiently blocked ciliogenesis, demonstrating the inhibitory effects of PAB on the Hh pathway at multiple levels. Thus, PAB may overcome drug-resistance induced by SMO mutations, which frequently occurs in clinical setting. PAB markedly suppressed tumor growth in the subcutaneous allografts of Ptch1 MB cells. Together, our results identified PAB as a potent Hh pathway inhibitor to treat Hh-dependent MB, especially cases resistant to SMO antagonists.
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http://dx.doi.org/10.1016/j.bcp.2021.114593DOI Listing
May 2021

ENB-guided microwave ablation combined with uniportal VATS for multiple ground glass opacities.

Ann Thorac Surg 2021 May 5. Epub 2021 May 5.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China. Electronic address:

Background: An increasing number of patients are being diagnosed with multiple ground glass opacities (GGOs), but a consensus on the treatment of these patients is still lacking. The aim of this study was to investigate the safety and feasibility of a novel technique, electromagnetic navigation bronchoscopy (ENB)-guided microwave ablation combined with uniportal video-assisted thoracoscopic surgery (Uni-VATS), in patients with multiple GGOs.

Methods: The clinical, radiographic, surgical, and pathological data of patients with multiple GGOs who underwent ENB-guided microwave ablation combined with Uni-VATS from October 2018 to December 2019 were reviewed.

Results: Eleven patients with multiple GGOs underwent ENB-guided microwave ablation combined with Uni-VATS, including 6 males and 5 females with a mean age of 61.3±5.1 (53-68) years. Thirty-seven lesions were observed in the 11 patients, 21 of which were microwave ablated and 16 of which were surgically resected. Only one patient developed postoperative pneumothorax and subcutaneous emphysema and was successfully discharged from the hospital after symptomatic treatment. The success rate and efficiency of microwave ablation under ENB guidance were 100%, with no other serious complications or procedure-related deaths occurring. No local metastasis or recurrence occurred in any patients during the follow-up period.

Conclusions: ENB-guided microwave ablation combined with Uni-VATS is safe and feasible in patients with multiple GGOs suspected of having multiple primary lung cancers, and may represent an alternative approach for more patients, particularly patients who cannot tolerate the simultaneous resection of multiple tumors.
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http://dx.doi.org/10.1016/j.athoracsur.2021.04.061DOI Listing
May 2021

Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis.

Cell Death Dis 2021 May 7;12(5):456. Epub 2021 May 7.

Department of Hematology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.

Chronic myelogenous leukemia (CML) is a clonal malignancy of hematopoietic stem cells featured with the fusion protein kinase BCR-ABL. To elicit the mechanism underlying BCR-ABL stability, we perform a screen against a panel of deubiquitinating enzymes (DUBs) and find that the ubiquitin-specific protease 7 (USP7) drastically stabilizes the BCR-ABL fusion protein. Further studies show that USP7 interacts with BCR-ABL and blocks its polyubiquitination and degradation. Moreover, USP7 knockdown triggers BCR-ABL degradation and suppresses its downstream signaling transduction. In line with this finding, genetic or chemical inhibition of USP7 leads to BCR-ABL protein degradation, suppresses BCR/ABL signaling, and induces CML cell apoptosis. Furthermore, we find the antimalarial artesunate (ART) significantly inhibits USP7/BCR-ABL interaction, thereby promoting BCR-ABL degradation and inducing CML cell death. This study thus identifies USP7 as a putative Dub of BCR-ABL and provides a rationale in targeting USP7/BCR-ABL for the treatment of CML.
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http://dx.doi.org/10.1038/s41419-021-03732-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105359PMC
May 2021

Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize.

PLoS One 2021 7;16(5):e0250349. Epub 2021 May 7.

College of Agronomy, Jilin Agricultural University, Changchun, China.

Gibberellin-dioxygenases genes plays important roles in the regulating plant development. However, Gibberellin-dioxygenases genes are rarely reported in maize, especially response to gibberellin (GA). In present study, 27 Gibberellin-dioxygenases genes were identified in the maize and they were classified into seven subfamilies (I-VII) based on phylogenetic analysis. This result was also further confirmed by their gene structure and conserved motif characteristics. And gibberellin-dioxygenases genes only occurred segmental duplication that occurs most frequently in plants. Furthermore, the gibberellin-dioxygenases genes showed different tissue expression pattern in different tissues and most of the gibberellin-dioxygenases genes showed tissue specific expression. Moreover, almost all the gibberellin-dioxygenases genes were significantly elevated in response to GA except for ZmGA2ox2 and ZmGA20ox10 of 15 gibberellin-dioxygenases genes normally expressed in leaves while 10 and 11 gibberellin-dioxygenases genes showed up and down regulated under GA treatment than that under normal condition in leaf sheath. In addition, we found that ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 might be potential genes for regulating balance of GAs which play essential roles in plant development. These findings will increase our understanding of Gibberellin-dioxygenases gene family in response to GA and will provide a solid base for further functional characterization of Gibberellin-dioxygenases genes in maize.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250349PLOS
May 2021

Complete Genome Sequence of Pseudomonas taiwanensis WRS8, a Plant Growth-Promoting and Biotite-Weathering Strain.

Microbiol Resour Announc 2021 May 6;10(18). Epub 2021 May 6.

Key Laboratory of Agrometeorology of Jiangsu Province, Department of Ecology, School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing, China.

WRS8 is a biotite-weathering organism isolated from wheat root. Here, we report its complete genome sequence, which may shed light on its role in plant growth promotion and biotite weathering.
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http://dx.doi.org/10.1128/MRA.00252-21DOI Listing
May 2021

A novel computational framework for genome-scale alternative transcription units prediction.

Brief Bioinform 2021 May 6. Epub 2021 May 6.

School of Mathematics, Shandong University, Jinan 250200, China.

Alternative transcription units (ATUs) are dynamically encoded under different conditions and display overlapping patterns (sharing one or more genes) under a specific condition in bacterial genomes. Genome-scale identification of ATUs is essential for studying the emergence of human diseases caused by bacterial organisms. However, it is unrealistic to identify all ATUs using experimental techniques because of the complexity and dynamic nature of ATUs. Here, we present the first-of-its-kind computational framework, named SeqATU, for genome-scale ATU prediction based on next-generation RNA-Seq data. The framework utilizes a convex quadratic programming model to seek an optimum expression combination of all of the to-be-identified ATUs. The predicted ATUs in Escherichia coli reached a precision of 0.77/0.74 and a recall of 0.75/0.76 in the two RNA-Sequencing datasets compared with the benchmarked ATUs from third-generation RNA-Seq data. In addition, the proportion of 5'- or 3'-end genes of the predicted ATUs, having documented transcription factor binding sites and transcription termination sites, was three times greater than that of no 5'- or 3'-end genes. We further evaluated the predicted ATUs by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses. The results suggested that gene pairs frequently encoded in the same ATUs are more functionally related than those that can belong to two distinct ATUs. Overall, these results demonstrated the high reliability of predicted ATUs. We expect that the new insights derived by SeqATU will not only improve the understanding of the transcription mechanism of bacteria but also guide the reconstruction of a genome-scale transcriptional regulatory network.
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http://dx.doi.org/10.1093/bib/bbab162DOI Listing
May 2021

Enhanced diversity and rock-weathering potential of bacterial communities inhabiting potash trachyte surface beneath mosses and lichens - A case study in Nanjing, China.

Sci Total Environ 2021 Apr 26;785:147357. Epub 2021 Apr 26.

Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.

Mosses and lichens have been shown to play an important role in enhancing global chemical weathering of the surface rock. However, there are no studies concerning the effects of mosses and lichens on the microbial communities inhabiting rock surfaces. In this study, culture-dependent and culture-independent analyses were employed to compare the diversity, composition, and rock-weathering activity of bacterial communities inhabiting potash trachyte surfaces covered by mosses (MR) and lichens (LR) with those inhabiting surrounding bare rock surfaces (BR). Analyses of 16S rRNA gene Miseq sequencing revealed that the order of alpha (α) diversity indices, in terms of the number of unique operational taxonomic units (OTUs) and Faith's index of phylogenetic diversity, was MR > LR > BR. Moreover, α-diveristy indices were positively correlated with the content of available phosphorus (AP) in rock samples (r = 0.87-0.92), and this explained 70% of the variation in bacterial community structure. The culture-dependent analyses revealed that 100% of the culturable bacterial strains could enhance potash trachyte weathering, and the order of rock-weathering acitivity of bacterial strains was MR > LR > BR. Acidolysis was found to be the major mechanism involved in the bacteria-mediated weathering of potash trachyte. Moreover, bacterial strians related to the genera Dyella and Ralstonia showed the highest rock-weatheirng activity, and both Dyella and Ralstonia were enriched in MR. The results of this study enhance our understanding of the roles of bacteria facilitated by mosses and lichens in rock weathering, element cycling, and soil formation, and provide new insights into the interaction between non-vascular plants and the bacteria on rock surfaces.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147357DOI Listing
April 2021

Clinical Outcomes and Prognostic Factors in Patients With Recurrent Patellar Lateral Dislocation Treated With Isolated Medial Patellofemoral Ligament Reconstruction: A Retrospective Single-Center Analysis.

Orthop J Sports Med 2021 Apr 12;9(4):2325967121995803. Epub 2021 Apr 12.

Medical School of Chinese PLA, Beijing, China.

Background: Medial patellofemoral ligament reconstruction (MPFLR) is widely used to treat patellofemoral instability. However, it is still unclear when a concomitant bony procedure is needed.

Purpose: To evaluate the therapeutic efficacy of isolated MPFLR for recurrent patellar lateral dislocation and to identify the prognostic factors for clinical outcomes.

Study Design: Case series; Level of evidence, 4.

Methods: A total of 237 patients who had undergone unilateral isolated MPFLR between January 2011 and December 2016 were included. Patellar tracking (J sign) and radiologic information including patellar height, trochlear dysplasia, tibial tubercle-trochlear groove distance, and patellar tilt was collected preoperatively, and the Kujala and International Knee Documentation Committee (IKDC) functional scores were used to assess postoperative recovery. Failure was defined as redislocation or patient-reported and clinically confirmed patellar instability. The influence of radiologic features and the J sign on knee functional scores was explored via subgroup analysis. Potential prognostic factors were explored using univariate and multivariate regression analyses, and risk factors for a positive J sign were evaluated using Spearman coefficient correlation analysis.

Results: The mean ± SD follow-up period was 70.67 ± 18.05 months (range, 36-108 months). From preoperatively to final follow-up, all patients had statistically and clinically significant improvements in the Kujala score (from 51.43 ± 6.87 to 88.52 ± 4.83; < .001) and IKDC score (from 49.78 ± 6.05 to 81.16 ± 4.20; < .001). Subgroup analysis indicated no significant difference in functional score improvements based on radiologic features or the J sign. Overall, 20 knees (8.4%) experienced postoperative failure: 11 with redislocation and 9 with patellar instability. A preoperative positive J sign was shown to be an independent prognostic factor for postoperative failure via logistic regression analyses: univariate (odds ratio, 3.340; 95% CI, 1.316-8.480; = .011) and multivariate (odds ratio, 3.038; 95% CI, 1.179-7.827; = .021). In addition, the J sign was closely associated with patellar height (, 0.159; = .014) and trochlear dysplasia (, 0.235; < .001).

Conclusion: Isolated MPFLR appears to be an effective strategy for recurrent patellar lateral dislocation, with significant functional improvement and a low failure rate. A preoperative positive J sign was identified as a potential prognostic risk factor for postoperative failure.
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http://dx.doi.org/10.1177/2325967121995803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044575PMC
April 2021

Different Metabolites in the Roots, Seeds, and Leaves of and Their Role in Alleviating Oxidative Stress.

J Anal Methods Chem 2021 15;2021:6628880. Epub 2021 Apr 15.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China.

In this study, we examined the metabolites from different parts of and their role in alleviating damage caused by oxidative stress. We used UHPLC-QTOF-MS to analyze the chemical components in the root, seed, and leaf extracts of . Two multivariate statistical analysis methods-namely, principal component analysis and partial least square discriminant analysis-were used to distinguish the samples obtained from different parts of the plant. Using univariate statistics, 130 different metabolites were screened out. Among these, the relative content of flavonoids and terpenoids was found to be highest in the leaves, the lignin and phenolic acid content was highest in the roots, and the amino acid and phenolic acid levels were highest in seeds. An MTT assay was used to test the anti-HO oxidative damage to PC12 cells in different parts of the sample. Lastly, using Pearson's correlation analysis, various metabolites from different parts of were correlated with their antioxidant effects from the corresponding parts. Fifty-two related different metabolites were found, of which 20 metabolites that were positively correlated to oxidative stress were present at a relatively higher level in the roots, whereas 32 metabolites that were negatively correlated were present at relatively higher levels in the seeds and leaves. The results of this study reveal the distribution characteristics and the antioxidant activity of different metabolites of and provide a reference for the rational development of its medicinal parts.
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http://dx.doi.org/10.1155/2021/6628880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064801PMC
April 2021

Anticancer and Anti-inflammatory Effect of Diosmin against Dalton Ascitic Lymphoma Induced Leukemia.

J Oleo Sci 2021 ;70(5):665-673

Department of Hematology, Yunnan Cancer Hospital.

Cancer is the world's biggest health problem and cancer-induced mortality happened all over the planet after the heart disease. The present study was to scrutinize the anti-leukemia effect of diosmin against Dalton Ascitic Lymphoma (DAL) induced leukemia in mice. DAL cell was used for induction the solid tumor. Body weight, life spans, tumor volume and mean survival time was estimated. Antioxidant, biochemical and pro-inflammatory cytokines were estimated. Diosmin showed the cell viability effect at dose dependent manner against the both cell lines. DAL induced solid tumor mice showed the decreased body weight, mean survival days, non viable cell count and increased the tumor volume, viable cell count and diosmin significantly (p < 0.001) reverse the effect of DAL. Diosmin significantly (p < 0.001) altered the hematological, differential leukocytes, antioxidant, biochemical, pro-inflammatory cytokines at dose dependently. Collectively, we can say that diosmin might alter the DAL induced abnormality via antioxidant and anti-inflammatory effects.
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http://dx.doi.org/10.5650/jos.ess21022DOI Listing
January 2021