Publications by authors named "Qi Li"

3,323 Publications

  • Page 1 of 1

Effects of a yeast-derived product on growth performance, antioxidant capacity, and immune function of broilers.

Poult Sci 2021 Jun 24;100(9):101343. Epub 2021 Jun 24.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

Yeast culture plus enzymatically hydrolyzed yeast cell wall (YC-EHY) contains crude protein, mannan-oligosaccharide, β-glucan and yeast culture. This study was carried out to explore the effects of dietary YC-EHY at different levels on growth performance, antioxidant capacity, and immune function of broiler chickens. A total of 320 one-day-age male broiler chicks were allocated into 4 groups and were fed with a basal diet supplemented with 0 mg/kg (the control group), 50 mg/kg, 100 mg/kg, 150 mg/kg YC-EHY for 42 d. Dietary YC-EHY improved average daily gain and feed efficiency during the starter, grower, and overall periods as well as average body weight of broiler chickens on 42 d (linear and quadratic, P < 0.05). Broiler chickens fed with YC-EHY quadratically increased jejunal sucrase activity on 21 d (quadratic, P < 0.05), and linearly and quadratically enhanced maltase activity on 21 and 42 d (linear and quadratic, P < 0.05). Supplementing YC-EHY linearly and quadratically enhanced jejunal superoxide dismutase (SOD) activity on 21 and 42 d and glutathione peroxidase (GPX) activity on 42 d whereas decreased malonaldehyde (MDA) concentration on 42 d (linear and quadratic, P < 0.05). Consistently, the jejunal genes expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and SOD1 on 21 and 42 d, heme oxygenase-1 (HO-1) and GPX1 on 42 d were enhanced by YC-EHY supplementation (linear and quadratic, P < 0.05). The concentrations of jejunal immunoglobulin G (IgG) on 21 and 42 d and secreted immunoglobulin A (SIgA) on 42 d were linearly and quadratically elevated by supplementing YC-EHY (linear and quadratic, P < 0.05). Dietary YC-EHY quadratically increased jejunal IgG and IgM genes expression on 21 d (quadratic, P < 0.05), and linearly and quadratically enhanced the genes expression of IgG and IgM on 42 d (linear and quadratic, P < 0.05). Overall, this study indicated that supplementing YC-EHY could exert beneficial effects on growth performance, intestinal antioxidant capacity and immune function in broiler chickens.
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http://dx.doi.org/10.1016/j.psj.2021.101343DOI Listing
June 2021

Characteristics, kinetics, thermodynamics and long-term effects of zerovalent iron/pyrite in remediation of Cr(VI)-contaminated soil.

Environ Pollut 2021 Jul 23;289:117830. Epub 2021 Jul 23.

School of Metallurgy and Environment, Central South University, Changsha, 410083, PR China; Chinese National Engineering Research Centre for Control & Treatment of Heavy Metal Pollution, Changsha, 410083, PR China. Electronic address:

Development of efficient, green and low-cost natural mineral-based reductive materials is promising to remediation of hexavalent chromium(Cr(VI))-contaminated soil. Considering the synergetic effect between pyrite and zerovalent iron (ZVI), an activated pyrite supported ZVI(ZVI/FeS) with high reducing activity was developed by ball milling activation of natural pyrite and sulfidation of ZVI. The remediation property of ZVI/FeS for Cr(VI)-contaminated soil was evaluated with different ZVI/FeS dosage, soil-water ratio, initial pH, time and temperature, as well as the stability of Cr. The results showed that ZVI/FeS possessed high reduction activity with soil Cr(VI) removal rate up to 99 % even under alkaline condition, and soil with different pH values eventually converged to neutral after 90 days, indicating that ZVI/FeS has a good self-regulating alkaline ability. The reduction process conformed to Langmuir-Hinshelwood first-order kinetics and was a spontaneous and endothermic process. The lower activation energy of 17.97 kJ mol (usually 60-250 kJ mol) indicated that the reduction reaction of Cr(VI) was particularly easy to occur. The speciation change of Cr in soil within 30 days demonstrated that the Cr in the soil was converted from a readily migratable state to a more stable state, where the Fe-Mn oxide bound fraction reached 85.03 % due to the generation of Cr(III)/Fe(III) co-precipitation. The results of long-term stability experiments showed that the leaching concentrations of Cr(VI) and total Cr decreased significantly after the ZVI/FeS treatment and remained stable at very low levels for 180 days. This study provided a sustainable way to fully utilize natural pyrite minerals to obtain iron-bearing reductive materials for feasible, effective and long-term stable immobilization of Cr(VI) in soil.
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http://dx.doi.org/10.1016/j.envpol.2021.117830DOI Listing
July 2021

A Holistic Approach of Extractables and Leachables Assessment of Rubber Stoppered Glass Vial Systems for Biotechnology Products.

J Pharm Sci 2021 Jul 26. Epub 2021 Jul 26.

Attribute Sciences, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320. Electronic address:

Rubber stoppered glass vial systems are widely used as primary containers for storing and delivering therapeutic protein products to patients. Addressing concerns and regulatory expectations related to the risk to biologic drug product quality and patient safety from rubber stoppered glass vial systems requires implementation of an extractable and leachable evaluation program based on material understanding, risk assessment, literature review, and a comprehensive scientifically sound analytical testing methodology. The extractable and leachable study design consisted of twelve drug products filled in twelve different size glass vials capped with laminated and nonlaminated rubber stoppers made from three different rubber formulations. Design of the model solvents was successful as they had little to no analytical interference and mimicked the formulation conditions and generated representative extractables capable of predicting leachables. The extraction conditions of time and temperature were appropriate as not to degrade the test materials or the extractable compounds, and yet generated significant quantities for identification of the extractable compounds with confidence. The extractables testing results were capable of predicting the leachable profiles of the twelve drug products. In each case, the leachable profile was a subset of the extractable profile. The organic and elemental impurities of the leachable profiles of drug products were the end-to-end verification of the quality of the glass vials, rubber stoppers and drug product lifecycles. Overall, the holistic approach was fully successful in the qualification of different vial systems as primary containers and delivery systems for different biotherapeutic products to ensure product quality and patient safety.
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http://dx.doi.org/10.1016/j.xphs.2021.07.015DOI Listing
July 2021

Design, Bio-evaluation and Molecular Dynamics Simulation of Novel GSK-3β Inhibitors.

Mol Inform 2021 Jul 29. Epub 2021 Jul 29.

School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, People's Republic of China.

Glycogen synthase kinase 3 beta (GSK-3β) is considered as a promising drug target for the treatment of Alzheimer's disease (AD). In the present study, two compound libraries were selected for virtual screening based on pharmacophore models of GSK-3β to discover new inhibitors. Nine potential hits were retained for biological investigation and four of these compounds showed GSK-3β inhibitory activity (with the IC values in sub-micromolar range on GSK-3β). Compounds 6 and 9 have good safety. They do not have any significant in vitro cytotoxicity against PC12 and SH-SY5Y neuroblastoma cells at concentrations up to 90 μM. Based on the inhibitory activity and druggability properties, compound 8 is the preferred molecule, and it is a promising lead for the development of the GSK-3β inhibitors for reducing the abnormal hyperphosphorylation of tau protein and relieving AD.
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http://dx.doi.org/10.1002/minf.202060031DOI Listing
July 2021

Publication Trends and Hot Spots in Chronic Postsurgical Pain (CPSP) Research: A 10-Year Bibliometric Analysis.

J Pain Res 2021 21;14:2239-2247. Epub 2021 Jul 21.

Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, People's Republic of China.

Purpose: Aging populations and increasing quality of life requirements have attracted growing efforts to study chronic postsurgical pain (CPSP). However, a diverse range of factors are involved in CPSP development, which complicates efforts to predict and treat this disease. To advance research in this field, our study aimed to use bibliometric analysis to quantitatively and qualitatively evaluate CPSP research and predict research hot spots over the last 10 years.

Methods: Relevant publications between 2011 and 2020 were extracted from the Web of Science Core Collection database. CiteSpace software (v5.7.R2) and the Online Analysis Platform of Literature Metrology were used to analyze research attributes including countries and authors, keywords and co-occurrence, and burst detection to predict trends and hot spots.

Results: A total of 2493 publications were collected with the number of annual publications showing nearly threefold increase over the past decade. Articles were the primary publication type with the United States as the leading country and the center of national collaboration. Johns Hopkins University provided the leading influence within the CPSP field. Postoperative pain, multimodal analgesia, quality of life, opioid, microglia, cesarean delivery, inguinal hernia, chronification, genetic polymorphism, and lidocaine were the top 10 clusters in co-occurrence cluster analysis. Moreover, burst detection was shown that epidural analgesia, nerve injury, total hip arthroplasty were the new hot spots within the CPSP field.

Conclusion: Bibliometric mapping not only defined the overall structure of CPSP-related research but its collective information provides crucial assistance to direct ongoing research efforts. The prominent keywords including "risk factor" and "multimodal analgesia" indicate that CPSP prevention and new treatment methods remain hot spots. Nonetheless, the recognition that CPSP is complex and changeable, proposes comprehensive biopsychosocial approaches are needed, and these will be essential to improve CPSP interventions and outcomes.
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http://dx.doi.org/10.2147/JPR.S300744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313379PMC
July 2021

Computed Tomography Morphological Classification of Lung Adenocarcinoma and Its Correlation with Epidermal Growth Factor Receptor Mutation Status: A Report of 1075 Cases.

Int J Gen Med 2021 21;14:3687-3698. Epub 2021 Jul 21.

Department of Radiology, University of Chinese Academy of Sciences Chongqing Renji Hospital (Fifth People's Hospital of Chongqing), Chongqing, 400062, People's Republic of China.

Background: Many delayed diagnoses of lung adenocarcinoma (LADC) are identified due to poor understanding of protean imaging findings. Moreover, clarifying the relationship between computed tomography (CT) morphological classification and epidermal growth factor receptor (EGFR) mutations of LADC might inform therapeutic decision-making while obtaining pathological specimens is difficult. Here, we retrospectively analyzed CT manifestations of LADC and investigated the morphological classification of tumors in relation to EGFR mutation status.

Methods: We included 1075 LADC patients undergoing chest CT and EGFR genotype examinations from January 2013 to January 2019. CT morphological characteristics of tumors were carefully evaluated and their correlation with EGFR mutation status was analyzed using the chi-squared test.

Results: Tumors were divided into eight types: I (peripheral solid nodule/mass; 526/1075, 48.93%), II (central solid nodule/mass; 220/1075, 20.47%), III (subsolid nodule/mass; 92/1075, 8.56%), IV (focal consolidation; 32/1075, 2.98%), V (cystic airspace; 14/1075, 1.30%), VI (multiple lesions with similar appearances to I-V; 85/1075, 7.91%), VII (diffuse consolidation; 53/1075, 4.93%), VIII (occult lesion usually obscured by nonobstructive atelectasis; 53/1075, 4.93%). Type III and IV tumors were more frequent in patients with EGFR mutation, whereas type II and VII tumors were more common in patients without EGFR mutation (all < 0.05). However, we did not identify any significant associations between other tumor types and EGFR mutation status (all > 0.05). Among patients with type VI tumors, EGFR mutation status was closely related to tumor density (all < 0.05). Furthermore, type VII tumors were associated with 19 deletion mutation positive and non-L858R mutation positive (all < 0.05).

Conclusion: LADC can be categorized into eight types based on CT imaging. Improving our understanding of the morphological classification and correlation with EGFR mutation status may contribute to the accurate diagnosis of LADC, while suggesting the presence of underlying EGFR genetic mutations.
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http://dx.doi.org/10.2147/IJGM.S316344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312332PMC
July 2021

Using a sulfur autotrophic fluidized bed reactor for simultaneous perchlorate and nitrate removal from water: S disproportionation prediction and system optimization.

Biodegradation 2021 Jul 27. Epub 2021 Jul 27.

College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, Henan, China.

The sulfur autotrophic reduction (SAR) process is promising in co-reduction of perchlorate and nitrate from aqueous solution. To further understand the reaction process, we developed a sulfur autotrophic fluidized bed reactor where the proceeding extent of sulfur (S) disproportionation was predicted by Response surface methodology (RSM) for the first time. Three fundamental reaction parameters including the hydraulic retention time (HRT), co-existing nitrate concentration ([Formula: see text]) and recirculation ratio (R) were considered for reactor optimization. The results demonstrated that S disproportionation was promoted by long HRT and high R, whereas was inhibited by high [Formula: see text]. Also, the optimal HRT, [Formula: see text] and R were 0.50 h, 10.00 mg/L and 14, respectively, the bioreactor can achieve high reduction efficiency of perchlorate and nitrate (> 98.45%), and generate less sulfate (236.07 mg/L). High-throughput sequencing showed that Chlorobaculum was related to S disproportionation, and Sulfurovum was associated with nitrate/perchlorate reducing. All results indicate that the sulfur autotrophic fluidized bed reactor is a promising candidate for the treatment of perchlorate and nitrate wastewater in future practical applications.
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http://dx.doi.org/10.1007/s10532-021-09957-8DOI Listing
July 2021

Copper-Catalyzed Intermolecular C(sp)-H Amination with Electrophilic -Benzoyl Hydroxylamines.

J Org Chem 2021 Jul 27. Epub 2021 Jul 27.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

A copper-catalyzed intermolecular electrophilic amination of benzamides with -benzoyl hydroxylamines was achieved with the assistance of an 8-aminoquinolyl group. With this protocol, good compatibility was observed for a variety of aryl amides and heteroaryl amides, and excellent tolerance with various functional groups was achieved. Significantly, the monoaminated product was overwhelmingly delivered under the simple reaction conditions. Preliminary mechanistic investigations suggested that a radical pathway should be excluded and C-H activation be potentially the rate-determining step.
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http://dx.doi.org/10.1021/acs.joc.1c01229DOI Listing
July 2021

Discovery of Cyclic Peptidomimetic Ligands Targeting the Extracellular Domain of EGFR.

J Med Chem 2021 Jul 23. Epub 2021 Jul 23.

Department of Chemistry, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida 33620, United States.

It is very promising to target the extracellular domain of epidermal growth factor receptor (EGFR) for developing novel and selective anticancer therapies. Herein, we report the discovery of a novel small molecule, , from a one-bead-two-compound (OBTC) cyclic γ-AApeptide library. The molecule was found to bind tightly to the extracellular domain of EGFR. Intriguingly, this molecule could also effectively antagonize EGF-stimulated EGFR phosphorylation and downstream signal transduction. Furthermore, together with its remarkable resistance to proteolytic degradation, was shown to effectively inhibit cell proliferation and migration in vitro and suppresses the growth of tumor in the A549 xenograft model in vivo, highlighting its potential therapeutic application for cancer treatment.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00607DOI Listing
July 2021

Enantioselective Synthesis of γ-Oxycarbonyl Motifs by Conjugate Addition of Photogenerated α-Alkoxy Radicals.

Org Lett 2021 Jul 23. Epub 2021 Jul 23.

Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.

Enantioselective catalytic Giese addition of photogenerated α-alkoxy radicals to acyl pyrazolidinones can be accomplished using a tandem Sc(III) Lewis acid/photoredox catalyst system. Surprisingly, the excited-state oxidation potential was not the only important variable, and the optimal photocatalyst was not the strongest oxidant screened. Our results show that both the oxidation and reduction potentials of the photocatalyst can be important for the reaction outcome, highlighting the importance of holistic considerations in designing photochemical reactions.
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http://dx.doi.org/10.1021/acs.orglett.1c01790DOI Listing
July 2021

Boosting the peroxidase-like activity of gold nanoclusters for the colorimetric detection of oxytetracycline in rat serum.

Analyst 2021 Jul 23. Epub 2021 Jul 23.

Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China. and School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P.R. China.

Gold nanoclusters (AuNCs)-based nanozymes have been studied widely as they provide unrivaled advantages in terms of preferable enzyme-like activities, high stability, and good biocompatibility. Although the enzyme-like catalytic activity of AuNCs has been the object of extensive investigation, understanding how charges or reactive oxygen species on the surfaces of AuNCs can enhance their catalytic performance in the colorimetric sensing of drugs by regulating the catalytic activity of AuNCs is still a big challenge. Herein, l-tryptophanonitrile (LTN)-protected AuNCs ([email protected]) were prepared, and their nanozyme activity was investigated in the catalytic oxidation process of the peroxidase substrate, namely 3,3',5,5'-tetramethylbenzidine, in the prescence of hydrogen peroxide. Oxytetracycline induced the aggregation of [email protected] due to the electrostatic interaction between the positively charged [email protected] and the negatively charged drug. Importantly, the aggregated [email protected] produced more reactive oxygen species and significantly boosted their peroxidase-like activity. Subsequently, a colorimetric method for highly specific and sensitive detection of oxytetracycline was establised. The ultraviolet-visible absorbance at a wavelength of 650 nm of the [email protected] exhibited a good linear relationship with oxytetracycline in a range of 0.5-15.0 μM (R2 = 0.994). The limit of detection was 0.3 μM. After oxytetracycline was abdominally injected in rats, the metabolic process of the drug in serums was further investigated by using the proposed sensing protocol. The improvable catalytic activity capability of the AuNCs-based nanozymes discloses its great potential in real bio-applications.
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http://dx.doi.org/10.1039/d1an01003dDOI Listing
July 2021

Geographic variations in the incidence of congenital hypothyroidism in China: a retrospective study based on 92 million newborns screened in 2013-2018.

Chin Med J (Engl) 2021 Jul 22. Epub 2021 Jul 22.

National Office for Maternal and Child Health Surveillance of China, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China National Center for Birth Defects Monitoring, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China. Department of Obsterics, Sichuan Birth Defects Clinical Research Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

Background: Although congenital hypothyroidism (CH) has been widely studied in Western countries, CH incidence at different administrative levels in China during the past decade remains unknown. This study aimed to update the incidence and revealed the spatial pattern of CH incidence in the mainland of China, which could be helpful in the planning and implementation of preventative measures.

Methods: The data used in our study were derived from 245 newborns screening centers that cover 30 provinces of the Chinese Newborn Screening Information System. Spatial auto-correlation was analyzed by Global Moran I and Getis-Ord Gi statistics at the provincial level. Kriging interpolation methods were applied to estimate a further detailed spatial distribution of CH incidence at city level throughout the mainland of China, and Kulldorff space scanning statistical methods were used to identify the spatial clusters of CH cases at the city level.

Results: A total of 91,921,334 neonates were screened from 2013 to 2018 and 42,861 cases of primary CH were identified, yielding an incidence of 4.66 per 10,000 newborns screened (95% confidence interval [CI]: 4.62-4.71). Neonates in central (risk ratio [RR] = 0.84, 95% CI: 0.82-0.85) and western districts (RR = 0.71, 95% CI: 0.69-0.73) had lower probability of CH cases compared with the eastern region. The CH incidence indicated a moderate positive global spatial autocorrelation (Global Moran I value = 0.394, P  < 0.05), and the CH cases were significantly clustered in spatial distribution. A most likely city-cluster (log-likelihood ratio [LLR] = 588.82, RR = 2.36, P  < 0.01) and 25 secondary city-clusters of high incidence were scanned. The incidence of each province and each city in the mainland of China was estimated by kriging interpolation, revealing the most affected province and city to be Zhejiang Province and Hangzhou city, respectively.

Conclusion: This study offers an insight into the space clustering of CH incidence at provincial and city scales. Future work on environmental factors need to focus on the effects of CH occurrence.
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http://dx.doi.org/10.1097/CM9.0000000000001613DOI Listing
July 2021

Photocatalytic Conversion of Methane: Recent Advancements and Prospects.

Angew Chem Int Ed Engl 2021 Jul 26. Epub 2021 Jul 26.

Hefei National Laboratory for Physical Sciences at the Microscale, Hefei National Laboratory for Physical Sciences at the Microscale, 96 Jinzhai Road, 230026, Hefei, CHINA.

Abundant and affordable methane is not only high-quality fossil energy, but also the raw material for the synthesis of value-added chemicals. Solar-energy-driven conversion of methane offers a promising approach to directly transform methane to valuable energy sources under mild conditions, but remains as a grand challenge currently. In this Review, recent advancements of photocatalytic conversion of methane are systematically summarized. Insights into the construction of effective semiconductor-based photocatalysts from the perspective of light-absorption units and active centers are proposed and discussed in detail. Moreover, photocatalytic performances of methane conversion over various catalysts are also presented and classified by oxidant systems. Lastly, challenges and future perspectives concerning mechanistic study and practical application of photocatalytic methane oxidation are introduced.
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http://dx.doi.org/10.1002/anie.202108069DOI Listing
July 2021

Cross-Sectional Survey of Clinical Trials of Stem Cell Therapy for Heart Disease Registered at ClinicalTrials.gov.

Front Cardiovasc Med 2021 8;8:630231. Epub 2021 Jul 8.

Department of International Medical Center/Ward of General Practice, West China Hospital, Sichuan University, Chengdu, China.

It is important to register clinical trials before their implementation. There is a lack of study to evaluate registered clinical trials of stem cell therapy for heart diseases. Our study used the registration information at ClinicalTrials.gov to provide an overview of the registered trials investigating stem cell therapy for heart diseases. We searched ClinicalTrials.gov from inception to October 1, 2020 to identify clinical trials evaluating stem cell therapy for heart diseases. These trials were included in a cross-sectional survey and descriptive analysis. The outcomes included start date, completion date, location, status, study results, funding, phase, study design, conditions, interventions, sex, age, and sample size of those trials, as well as conditions, efficacy, safety and samples of the publications. SPSS 24.0 software was used for the statistical analysis. A total of 241 trials were included. The registration applications for most trials originated from the United States, and the research start date ranged from 2001 to 2025. More than half of the trials have been completed, but few trials have published results (15.62%). The funding source for 81.12% of trials was recorded as "other" because the specific funding source was not indicated. There were 226 (93.78%) interventional studies and 15 (6.22%) observational studies; among all 241 studies, only 2.90% were phase 4 trials. Most interventional studies used randomized allocation, parallel assignment, and blinding. Of the observational studies, 6 were cohort studies (40.00%) and 73.33% were prospective. The most common disease was coronary artery disease (57.68%) and 98.34% included both male and female participants. The sample size included fewer than 50 patients in 58.51% of trials, and only 18 trials (7.47%) lasted more than 121 months. The registered details were illogical for nine trials (3.8%) that included 0 subjects and two trials (0.8%) that had a duration of 0 months (0.8%). In term of publications of the trials, most of the publications of the trials showed efficacy and safety in stem cell therapy for heart disease. The clinical trials investigating stem cell therapy for heart diseases registered at ClinicalTrials.gov are mostly interventional studies, and only a few are phase 4 trials. Most trials have a small sample size, and few have a duration of more than 121 months. Most of the completed trials did not publish their results, and some of the registration information was incomplete and illogical.
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http://dx.doi.org/10.3389/fcvm.2021.630231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295466PMC
July 2021

[Interpretation of the 2019 American Academy of Audiology "Clinical Cochlear Implantation Clinical Practice Guide": patient selection and indications].

Authors:
Qi Li

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Jun;35(6):491-494

In 2019, American Academy of Audiology published the clinical practice guideline for cochlear implant based on evidence-based methodology. The primary purposes of this guideline were to provide a set of statements, recommendations, and strategies for cochlear implant. This paper aimed at interpreting the guideline with a focus on patient selection and indications sections. The determination of candidacy for a cochlear implant involves a series of tests, including audiometric testing, electrophysiology, fitting and verification of amplification, speech perception, and a solid understanding of the impact of hearing loss on educational, vocational, and psychosocial outcomes, as well as speech and language skills. The aspects of the pre-operative process for determining candidacy for a cochlear implant are described in this paper.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.06.002DOI Listing
June 2021

Cholecystectomy as a risk factor for metabolic dysfunction-associated fatty liver disease: unveiling the metabolic and chronobiologic clues behind the bile acid enterohepatic circulation.

J Physiol Biochem 2021 Jul 24. Epub 2021 Jul 24.

Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Heping District, Shenyang, 110004, Liaoning Province, China.

Metabolic dysfunction-associated fatty liver disease (MAFLD) is highly prevalent worldwide. Recent clinical and experimental studies have addressed the association between cholecystectomy and MAFLD, confirming that cholecystectomy is an independent risk factor for MAFLD. In this review, we describe the epidemiologic evidence that links cholecystectomy to MAFLD, and discuss the possible mechanisms behind these connections, in order to unveil the metabolic and chronobiologic signals conveyed by the waves of the bile acid enterohepatic circulation.
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http://dx.doi.org/10.1007/s13105-020-00782-wDOI Listing
July 2021

and , Two New Records on in China.

Mycobiology 2021 17;49(3):267-274. Epub 2021 May 17.

Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, China.

Two species isolated from fruit of in China were characterized based on morphology and multilocus phylogeny of ITS, , and gene sequences. The phylogeny indicated that the two species match and . A critical examination of phenotypic characteristics confirmed the phylogenetic results. was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of . To our knowledge, the two species were firstly reported on in China.
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http://dx.doi.org/10.1080/12298093.2021.1912254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259869PMC
May 2021

Author Correction: Adductor canal block versus femoral nerve block for total knee arthroplasty: a meta-analysis of randomized controlled trials.

Sci Rep 2021 Jul 21;11(1):15230. Epub 2021 Jul 21.

Department of Orthopedics, West China Hospital/West China School of Medicine, Sichuan University, Chengdu, 610041, People's Republic of China.

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http://dx.doi.org/10.1038/s41598-021-94766-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295281PMC
July 2021

Synergistic chromium(VI) reduction and phenol oxidative degradation by FeS/Fe and persulfate.

Chemosphere 2021 Oct 26;281:130957. Epub 2021 May 26.

Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Lushan South Road 932, Changsha, Hunan, 410083, PR China; Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, Changsha, 410083, PR China. Electronic address:

It is a challenge to simultaneously treat the combined pollutants of chromium(VI) (Cr(VI)) and organics (such as phenol) in wastewater. Here, a stable and efficient redox system based on FeS sulfidated zero valent iron (FeS/Fe) and persulfate (PS) was developed to synchronously remove Cr(VI) and phenol. 100% of phenol (10 mg/L) was oxidized in 10 min and Cr(VI) (20 mg/L) was completely reduced to Cr(III) in 90 min in the FeS/Fe+PS system with a pH range of 3.0-9.0, respectively. phenol was selectively oxidized without re-oxidizing Cr(III) in such system. The surface-bound Fe was the major reactive species to synchronously reduce Cr(VI) and oxidize phenol. The mechanisms were elucidated that the phenol degradation was accelerated by the generated Cr(III) complexing with its products, and that SO, whose production speed was accelerated by the PS activation to oxidize phenol and FeS, was conductive to corrode Fe to regenerate the surface-bound Fe for reducing Cr(VI) and oxidizing phenol. It is potential to develop a high-performance and large-scaled FeS/Fe-based redox platform to remediate the complex pollution of Cr(VI) and organics.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130957DOI Listing
October 2021

Microheterogeneous Distribution of Hydroxyl Radicals in Illuminated Dissolved Organic Matter Solutions.

Environ Sci Technol 2021 Jul 21. Epub 2021 Jul 21.

Department of Environmental Science & Engineering, Fudan University, Shanghai 200438, P. R. China.

Hydroxyl radicals (OH) are important reactive species that are photochemically generated through solar irradiation of chromophoric dissolved organic matter (CDOM) in surface waters. However, the spatial distribution within the complex three-dimensional structure of CDOM has not been examined. In this study, we used a series of hydrophobic chlorinated paraffins as chemical probes to elucidate the microheterogeneous distribution of OH in illuminated CDOM solutions. The steady-state concentration of OH inside the CDOM microphase is 210 ± 31-fold higher than the concentration in the aqueous phase. Our results suggest that the most photochemically generated OH are confined into the CDOM microphase. Thus, illuminated CDOM behaves as a natural microreactor for OH-based oxidations. By including intra-CDOM OH, the quantum yield of OH for CDOM solutions was estimated to be 2.2 ± 0.5 × 10, which is 2 orders of magnitude greater than previously thought. The elevated concentrations of photogenerated OH within the CDOM microphase may improve the understanding of hydrophobic pollutant degradation in aqueous environments. Moreover, our results also suggest that OH oxidation may play more important roles in the phototransformation of CDOM than previously expected.
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http://dx.doi.org/10.1021/acs.est.1c03346DOI Listing
July 2021

A Systematic Thermal Analysis for Accurately Predicting the Extrusion Printability of Alginate-Gelatin-Based Hydrogel Bioinks.

Int J Bioprint 2021 22;7(3):394. Epub 2021 Jun 22.

State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310058, People's Republic of China.

Three-dimensional (3D) bioprinting has significant potential for addressing the global problem of organ shortages. Extrusion printing is a versatile 3D bioprinting technique, but its low accuracy currently limits the solution. This lack of precision is attributed largely to the complex thermal and dynamic properties of bioinks and makes it difficult to provide accurate estimations of the printed results. It is necessary to understand the relationship between printing temperature and materials' printability to address this issue. This paper proposes a quantitative thermal model incorporating a system's printing temperatures (syringe, ambient, and bioink) to facilitate accurate estimations of the printing outcomes. A physical model was established to reveal the relationship between temperature, pressure, and velocity in guiding the printing of sodium alginate-gelatin composite hydrogel (a popular bioink) to optimize its extrusion-based printability. The model considered the phenomenon of bioink die swells after extrusion. A series of extrusion experiments confirmed that the proposed model offers enhanced printing outcome estimations compared with conventional models. Two types of nozzles (32- and 23-gauge) were used to print several sets of lines with a linewidth step of 50 mm by regulating the extrudate's temperature, pressure, and velocity separately. The study confirmed the potential for establishing a reasonable, accurate open-loop linewidth control based on the proposed optimization method to expand the application of extrusion-based bioprinting further.
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http://dx.doi.org/10.18063/ijb.v7i3.394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287498PMC
June 2021

Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production.

Sci Rep 2021 Jul 20;11(1):14779. Epub 2021 Jul 20.

Beijing Key Laboratory of Nutrition and Health and Food Safety, Nutrition and Health Research Institute, COFCO, Beijing, 102209, People's Republic of China.

Saccharopolyspora spinosa is a well-known actinomycete for producing the secondary metabolites, spinosad, which is a potent insecticides possessing both efficiency and safety. In the previous researches, great efforts, including physical mutagenesis, fermentation optimization, genetic manipulation and other methods, have been employed to increase the yield of spinosad to hundreds of folds from the low-yield strain. However, the metabolic network in S. spinosa still remained un-revealed. In this study, two S. spinosa strains with different spinosad production capability were fermented and sampled at three fermentation periods. Then the total RNA of these samples was isolated and sequenced to construct the transcriptome libraries. Through transcriptomic analysis, large numbers of differentially expressed genes were identified and classified according to their different functions. According to the results, spnI and spnP were suggested as the bottleneck during spinosad biosynthesis. Primary metabolic pathways such as carbon metabolic pathways exhibited close relationship with spinosad formation, as pyruvate and phosphoenolpyruvic acid were suggested to accumulate in spinosad high-yield strain during fermentation. The addition of soybean oil in the fermentation medium activated the lipid metabolism pathway, enhancing spinosad production. Glutamic acid and aspartic acid were suggested to be the most important amino acids and might participate in spinosad biosynthesis.
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http://dx.doi.org/10.1038/s41598-021-94251-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292330PMC
July 2021

Comparison of Intra-Abdominal Pressure Measurements in Critically Ill Patients Using Intravesical Normal Saline at 15°C, 25°C, and 35°C.

Med Sci Monit 2021 Jul 21;27:e932804. Epub 2021 Jul 21.

Department of Critical Care Medicine, Sixth Medical Center of People's Liberation Army (PLA) General Hospital, Beijing, China (mainland).

BACKGROUND The incidence of intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) in intensive care units is high. Dynamic monitoring of intra-abdominal pressure (IAP) is important to treat patients with these conditions. The World Society of Abdominal Compartment Syndrome revised IAP measurement and treatment guidelines in 2013. IAP is measured by instilling ≤25 mL of sterile saline into the bladder, but there is no requirement for the saline to be at a specific temperature. Many doctors presume that using cold saline will trigger bladder muscle spasms, resulting in measurement error. In the present study, we investigated the effect of body-temperature saline on IAP measurements. MATERIAL AND METHODS A single-center study was conducted in 12 patients with IAH over a 2-year period. IAP was measured via the bladder with instillation of sterile saline at temperatures of 35°C, 25°C, and 15°C. We analyzed the data using R software, version 4.1.0. Paired t tests were used for comparisons between groups. A Spearman rank correlation analysis was performed to compare groups. Analysis results were plotted using the R packages ggplot2, ggpubr, and BlandAltmanLeh. P<0.05 was considered statistically significant. RESULTS There was a significant difference in IAP measurement between the 15°C and 35°C groups (t=-2.55, P=0.027). There was no significant difference between the 25°C and 35°C groups (t=0.73, P=0.48). Bland-Altman analysis showed that IAP was consistent in the 25°C and 35°C groups. CONCLUSIONS Although it is preferable to measure IAP with saline at body temperature (35°C), a temperature >25°C is associated with accurate results. Using saline at <15°C should be avoided.
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http://dx.doi.org/10.12659/MSM.932804DOI Listing
July 2021

Identification of diatom taxonomy by a combination of region-based full convolutional network, online hard example mining, and shape priors of diatoms.

Int J Legal Med 2021 Jul 20. Epub 2021 Jul 20.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University & Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Zhongshan 2nd Road 74, Yuexiu District, Guangzhou, People's Republic of China.

Diatom test is one of the commonly used diagnostic methods for drowning in forensic pathology, which provides supportive evidence for drowning. However, in forensic practice, it is time-consuming and laborious for forensic experts to classify and count diatoms, whereas artificial intelligence (AI) is superior to human experts in processing data and carrying out classification tasks. Some AI techniques have focused on searching diatoms and classifying diatoms. But, they either could not classify diatoms correctly or were time-consuming. Conventional detection deep network has been used to overcome these problems but failed to detect the occluded diatoms and the diatoms similar to the background heavily, which could lead to false positives or false negatives. In order to figure out the problems above, an improved region-based full convolutional network (R-FCN) with online hard example mining and the shape prior of diatoms was proposed. The online hard example mining (OHEM) was coupled with the R-FCN to boost the capacity of detecting the occluded diatoms and the diatoms similar to the background heavily and the priors of the shape of the common diatoms were explored and introduced to the anchor generation strategy of the region proposal network in the R-FCN to locate the diatoms precisely. The results showed that the proposed approach significantly outperforms several state-of-the-art methods and could detect the diatom precisely without missing the occluded diatoms and the diatoms similar to the background heavily. From the study, we could conclude that (1) the proposed model can locate the position and identify the genera of common diatoms more accurately; (2) this method can reduce the false positives or false negatives in forensic practice; and (3) it is a time-saving method and can be introduced.
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http://dx.doi.org/10.1007/s00414-021-02664-2DOI Listing
July 2021

Circulating bile acid profiles: a need for further examination.

J Clin Endocrinol Metab 2021 Jul 19. Epub 2021 Jul 19.

Department of General Surgery, Shengjing Hospital of China Medical University, Heping District, Shenyang, Liaoning Province, China.

Context: Bile acids (BAs) are increasingly recognized as metabolic and chronobiologic integrators that synchronize the systemic metabolic response to nutrient availability. Alterations in the concentration and/or composition of circulating BAs are associated with a number of metabolic disorders, such as obesity, type 2 diabetes mellitus (T2DM), insulin resistance (IR), and metabolic-associated fatty liver disease (MAFLD). This review summarizes recent evidence that links abnormal circulating BA profiles to multiple metabolic disorders, and discusses the possible mechanisms underlying the connections to determine the role of BA profiling as a novel biomarker for these abnormalities.

Evidence Acquisition: The review is based on a collection of primary and review literature gathered from a PubMed search of BAs, T2DM, IR, and MAFLD, among other keywords.

Evidence Synthesis: Obese and IR subjects appear to have elevated fasting circulating BAs but lower postprandial increase when compared with controls. The possible underlying mechanisms are the disruption in the synchronization between the feeding/fasting cycle and the properties of BA-regulated metabolic pathways. Whether BA alterations are associated per se with MAFLD remains inconclusive. However, increased fasting circulating BAs level was associated with higher risk of advanced fibrosis stage. Thus, for patients with MAFLD, dynamically monitoring the circulating BA profiles may be a promising tool for the stratification of MAFLD.

Conclusions: Alterations in the concentration, composition, and rhythm of circulating BAs are associated with adverse events in systemic metabolism. Subsequent investigations regarding these aspects of circulating BA kinetics may help predict future metabolic disorders and guide therapeutic interventions.
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http://dx.doi.org/10.1210/clinem/dgab531DOI Listing
July 2021

IDH2 contributes to tumorigenesis and poor prognosis by regulating m6A RNA methylation in multiple myeloma.

Oncogene 2021 Jul 17. Epub 2021 Jul 17.

Department of Cell Biology, School of Biology & Basic Medical Sciences, Soochow University, Suzhou, China.

Epigenetic alterations have been previously shown to contribute to multiple myeloma (MM) pathogenesis via DNA methylations and histone modifications. RNA methylation, a novel epigenetic modification, is required for cancer cell survival, and targeting this pathway has been proposed as a new therapeutic strategy. The extent to the N6-methyladenosine (m6A)-regulatory pathway functions in MM remains unknown. Here, we show that an imbalance of RNA methylation may underlies the tumorigenesis of MM. Mechanistically, isocitrate dehydrogenase 2 (IDH2) is highly expressed in CD138 cells from MM and its levels appear a progressive increase in the progression of plasma cell dyscrasias. Downregulation of IDH2 increases global m6A RNA levels and reduces myeloma cell growth in vitro, decreases the burden of disease and prolongs overall survival in vivo. IDH2 regulates RNA methylation by activating the RNA demethylase FTO, which is an α-KG-dependent dioxygenase. Furthermore, IDH2-mediated FTO activation decreases the m6A level on WNT7B transcripts, then increases WNT7B expression and thus activated Wnt signaling pathway. Moreover, survival analysis indicates that the elevated expression of IDH2 predicts a poor prognosis. Higher expression of FTO is related to higher International Staging System (ISS) stage and higher Revised-ISS (R-ISS) stage of MM. Collectively, our studies reveal that IDH2 regulates global m6A RNA modification in MM via targeting RNA demethylases FTO. The imbalance of m6A methylation activates the Wnt signaling pathway by enhancing the WNT7B expression, and thus promoting tumorigenesis and progression of MM. IDH2 might be used as a therapeutic target and a possible prognostic factor for MM.
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http://dx.doi.org/10.1038/s41388-021-01939-7DOI Listing
July 2021

Which can explain the pip-and-pop effect during a visual search: Multisensory integration or the oddball effect?

J Exp Psychol Hum Percept Perform 2021 May;47(5):689-703

School of Psychology, Liaoning Normal University.

In a dynamic search task, participants were instructed to search for a visual target among various distractors. An auditory stimulus presented in synchrony with a color change of the visual target can improve the efficiency of the search, which is known as the pip-and-pop effect. Some researchers have suggested that the underlying mechanism of this effect is multisensory integration, while others have thought it is due to an oddball stimulation that attracts attention. Utilizing the eye movement technique, we conducted 3 experiments to investigate the pip-and-pop effect and its causes. In Experiment 1, we used a static search paradigm and controlled for the presence or absence of sound cues when the visual target was presented. It turned out that sound had no effect on a static search; that is, no pip-and-pop effect was found. In Experiment 2, we used a dynamic search paradigm in which the participants were instructed to search for vertical or horizontal line segments among various directional distractor segments and as the color of individual segments changed randomly during the stimulus presentation, with and without the sound cue. We found that the pip-and-pop effect was present. The irrelevant sound reduced the mean fixation number and expanded the mean saccade amplitude. In Experiment 3, we also used a dynamic search paradigm and controlled the frequency of synchronization between the sound and the target color change. There were 3 types of sound conditions: No-sound, 1-sound, and 2-sound. The results suggest that as the frequency of synchronization between the sound and the target color change increases (2-sound condition), the pip-and-pop effect also increases. Therefore, we propose that the pip-and-pop effect is more likely to result from the attention attracted by oddball stimuli than from the multisensory integration of synchronized auditory-visual stimuli. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
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http://dx.doi.org/10.1037/xhp0000905DOI Listing
May 2021

Evolution of the interactions between GII.4 noroviruses and histo-blood group antigens: Insights from experimental and computational studies.

PLoS Pathog 2021 Jul 12;17(7):e1009745. Epub 2021 Jul 12.

The Sixth Laboratory, National Vaccine and Serum Institute, Beijing, China.

Norovirus (NoV) is the major pathogen causing the outbreaks of the viral gastroenteritis across the world. Among the various genotypes of NoV, GII.4 is the most predominant over the past decades. GII.4 NoVs interact with the histo-blood group antigens (HBGAs) to invade the host cell, and it is believed that the receptor HBGAs may play important roles in selecting the predominate variants by the nature during the evolution of GII.4 NoVs. However, the evolution-induced changes in the HBGA-binding affinity for the GII.4 NoV variants and the mechanism behind the evolution of the NoV-HBGA interactions remain elusive. In the present work, the virus-like particles (VLPs) of the representative GII.4 NoV stains epidemic in the past decades were expressed by using the Hansenula polymorpha yeast expression platform constructed by our laboratory, and then the enzyme linked immunosorbent assay (ELISA)-based HBGA-binding assays as well as the molecular dynamics (MD) simulations combined with the molecular mechanics/generalized born surface area (MMGBSA) calculations were performed to investigate the interactions between various GII.4 strains and different types of HBGAs. The HBGA-binding assays show that for all the studied types of HBGAs, the evolution of GII.4 NoVs results in the increased NoV-HBGA binding affinities, where the early epidemic strains have the lower binding activity and the newly epidemic strains exhibit relative stronger binding intensity. Based on the MD simulation and MMGBSA calculation results, a physical mechanism that accounts for the increased HBGA-binding affinity was proposed. The evolution-involved residue mutations cause the conformational rearrangements of loop-2 (residues 390-396), which result in the narrowing of the receptor-binding pocket and thus tighten the binding of the receptor HBGAs. Our experimental and computational studies are helpful for better understanding the mechanism behind the evolution-induced increasing of HBGA-binding affinity, which may provide useful information for the drug and vaccine designs against GII.4 NoVs.
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http://dx.doi.org/10.1371/journal.ppat.1009745DOI Listing
July 2021

Trends in cancer mortality in China from 2004 to 2018: A nationwide longitudinal study.

Cancer Commun (Lond) 2021 Jul 12. Epub 2021 Jul 12.

Shanghai East Hospital, Key Laboratory of Arrhythmias, Ministry of Education, Tongji University School of Medicine, Tongji University, Shanghai, 200120, P. R. China.

Background: The long-term trend in cancer death in a rapidly developing country provides information for cancer prophylaxis. Here, we aimed to identify the trends in cancer mortality in China during the 2004-2018 period.

Methods: Using raw data from the national mortality surveillance system of China, we assessed the mortalities of all cancer and site-specific cancers during the 2004-2018 period. The participants were divided into three age groups: ≥65 years, 40-64 years, and ≤39 years. Changing trends in cancer death by gender, residency, and tumor location were estimated using fitting joinpoint models to log-transformed crude mortality rates (CMRs) and age-standardized mortality rates (ASMRs).

Results: Cancer death accounted for 24% of all-cause of death in China during 2014-2018. The CMR of all cancer was 150.0 per 100,000 persons. Cancer was the leading cause of death in the population <65 years. The six major cancer types (lung/bronchus cancer, liver cancer, stomach cancer, esophagus cancer, colorectal cancer, and pancreas cancer) accounted for 75.85% of all cancer deaths. The CMR of all cancer increased while the ASMR decreased during 2014-2018 (P < 0.001). Lung/bronchus cancer and liver cancer were the leading causes of cancer death in the population <65 years, accounting for 45.31% (CMR) and 44.35% (ASMR) of all cancer death, respectively. The ASMR of liver cancer was higher in the 40-64 years population than in the ≥65 years population, in contrast to the other five major cancers. The ASMRs of liver cancer, stomach cancer, and esophagus cancer decreased although they were higher in rural residents than in urban residents; the ASMRs of lung/bronchus cancer, colorectal cancer, and pancreas cancer increased in rural residents although they were higher in urban residents than in rural residents during 2014-2018.

Conclusion: Although the ASMR of all cancer decreased in China during 2004-2018, lung/bronchus cancer and liver cancer remained the leading causes of cancer-related premature death. Lung/bronchus cancer, colorectal cancer, and pancreas cancer increased in rural residents.
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http://dx.doi.org/10.1002/cac2.12195DOI Listing
July 2021

POFUT1 acts as a tumor promoter in glioblastoma by enhancing the activation of Notch signaling.

J Bioenerg Biomembr 2021 Jul 12. Epub 2021 Jul 12.

Department of Ultrasonography, Xi'an People's Hospital (Xi'an Fourth Hospital), No. 21 Jiefang Road, Xi'an, 710004, Shaanxi, China.

Dysregulation of protein O-fucosyl transferase 1 (POFUT1) contributes to the occurrence and progression of multiple cancers. However, whether POFUT1 has a relationship with the pathogenesis of glioblastoma (GBM) is unknown. This work was aimed at evaluating the detailed relevance of POFUT1 in GBM. Here, we demonstrated high levels of POFUT1 in GBM tissue and elucidated that GBM patients with high levels of POFUT1 had a shorter survival rate than those with low levels of POFUT1. POFUT1 knockdown in GBM cells markedly downregulated the ability to proliferate and invade, while overexpression of POFUT1 potentiated the proliferative and invasive ability of GBM cells. Further mechanistic studies indicated that silencing POFUT1 prohibited the activation of Notch signaling, leading to a reduction in the expression of HES1 and HEY1. On the contrary, overexpression of POFUT1 enhanced the activation of Notch signaling. Notably, inhibition of Notch signaling markedly reversed POFUT1-overexpression-induced tumor promotion effects in GBM cells. In addition, POFUT1 silencing markedly repressed the potential of GBM cells to form tumors in vivo. In conclusion, the data of this work indicates that POFUT1 serves a tumor promotion role in GBM by enhancing the activation of Notch signaling. This study underlines the potential role of the POFUT1/Notch axis in GBM progression and proposes POFUT1 as a promising anticancer target for GBM.
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http://dx.doi.org/10.1007/s10863-021-09912-5DOI Listing
July 2021
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