Publications by authors named "Qi Fan"

272 Publications

Comprehensive Analysis of Differentially Expressed Genes and Epigenetic Modification-Related Expression Variation Induced by Saline Stress at Seedling Stage in Fiber and Oil Flax, L.

Plants (Basel) 2022 Aug 5;11(15). Epub 2022 Aug 5.

Faculty of Agronomy, Jilin Agricultural University, Changchun 131018, China.

The ability of different germplasm to adapt to a saline-alkali environment is critical to learning about the tolerance mechanism of saline-alkali stress in plants. Flax is an important oil and fiber crop in many countries. However, its molecular tolerance mechanism under saline stress is still not clear. In this study, we studied morphological, physiological characteristics, and gene expression variation in the root and leaf in oil and fiber flax types under saline stress, respectively. Abundant differentially expressed genes (DEGs) induced by saline stress, tissue/organ specificity, and different genotypes involved in plant hormones synthesis and metabolism and transcription factors and epigenetic modifications were detected. The present report provides useful information about the mechanism of flax response to saline stress and could lead to the future elucidation of the specific functions of these genes and help to breed suitable flax varieties for saline/alkaline soil conditions.
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http://dx.doi.org/10.3390/plants11152053DOI Listing
August 2022

Distribution of pediatric keratoconus by different age and gender groups.

Front Pediatr 2022 18;10:937246. Epub 2022 Jul 18.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou, China.

Purpose: To evaluate the distribution of pediatric keratoconus patients and the disease severity based on different age and gender groups in China.

Materials And Methods: A total of 446 keratoconus eyes in 266 pediatric patients from January 2019 to January 2022 were included in the cross-sectional study. The clinical findings and severity of keratoconus were recorded and Kruskal-Wallis test, chi-squared test, and Cochran-Mantel-Haenszel (CMH) test were used to compare the parameters between different gender and age groups.

Results: The male/female ratio was 353/93 (3.8:1), and the median age was 16 years (range: 6-17 years). Male patients were statistically younger than female patients ( = 0.041). The male/female ratio decreased with age ( for trend = 0.011). The distribution of the topographic keratoconus classification (TKC) stage was significantly different between gender and age groups (all < 0.05). Male patients had a higher ratio of advanced keratoconus eyes (TKC ≥ 3) than female patients ( < 0.001), and CMH analysis indicated that being a male was a risk factor for advanced keratoconus after controlling for age (odds ratio: 2.581, < 0.001).

Conclusion: Male keratoconus patients were younger, with a higher ratio of advanced keratoconus than female patients in the Chinese pediatric patients evaluated. Multicenter studies with larger sample sizes are necessary in the future.
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http://dx.doi.org/10.3389/fped.2022.937246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339668PMC
July 2022

Production and Selection of Antibody-Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues.

Biosensors (Basel) 2022 Jul 24;12(8). Epub 2022 Jul 24.

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

To produce a sensitive monoclonal antibody (mAb) for the simultaneous detection of carbofuran, benfuracarb, carbosulfan and 3-hydroxy-carbofuran, 2,3-dihydro-2,2-dimethyl-7-benzofuranmethanamine (DDB) was conjugated to bovine serum albumin (BSA) to prepare the immunogen DDB-BSA and mice were immunized. Coating antigens were prepared by conjugating DDB and 5-methoxy-2,3-dihydrobenzofuran-3-acetic acid (MDA) to BSA and ovalbumin (OVA), respectively. Furthermore, the effect of different antibody-antigen pairs on the sensitivity of ELISA and LFIA methods for the detection of carbofuran was investigated. After the immunization, a high-affinity mAb 13C8 was obtained. The ability of the coating antigen to compete with carbofuran for binding antibodies was found to be significantly different between ELISA and LFIA methods. With the antibody-antigen pair 13C8-MDA-OVA, the IC values of the ELISA and QD-LFIA methods for carbofuran were 0.18 ng/mL and 0.67 ng/mL, respectively. The cross-reactivity (CR) values of the two methods for benfuracarb, carbosulfan and 3-hydroxy-carbofuran ranged from 72.0% to 83.7%, while, for other carbamate pesticides, the CR values were less than 1%. The spiked recoveries of carbofuran in vegetables by the QD-LFIA method were 83-111%, with a coefficient of variation below 10%, and the test results of the actual samples were consistent with the HPLC-MS method. Overall, this study provides key materials for the development of immunoassays for carbofuran and its analogues, and the antibody-antigen pair selection strategy established in this study provides useful insights for the development of sensitive immunoassays for other compounds.
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http://dx.doi.org/10.3390/bios12080560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332470PMC
July 2022

Morphological and Phylogenetic Characterization Reveals Five New Species of (Cordycipitaceae, Hypocreales).

J Fungi (Basel) 2022 Jul 19;8(7). Epub 2022 Jul 19.

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650091, China.

is a very important fungal resource, with some species in the genus having great medical, economic and ecological value. This study reports five new species of from Yunnan Province and Guizhou Province in Southwestern China and Dole Province in Vietnam, providing morphological descriptions, illustrations, phylogenetic placements, associated hosts and comparisons with allied taxa. Based on morphological observations and phylogenetic analyses of combined nr, nr, , and sequence data, it was determined that these five new species were located in the clade of and different from other species of . The five novel species had morphologies similar to those of other species in the genus, with bright orange cylindrical to clavate stromata (gregarious). The fertile part lateral sides usually had a longitudinal ditch without producing perithecia, and superficial perithecia. The phialides had a swollen basal portion, tapering abruptly into a narrow neck and oval or fusiform one-celled conidia, often in chains. The morphological characteristics of 23 species in , including five novel species and 18 known taxa, were also compared in the present study.
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http://dx.doi.org/10.3390/jof8070747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321185PMC
July 2022

Overexpression of from Fragrant Woodfern () Enhances High-Temperature Tolerance in Tobacco ().

Genes (Basel) 2022 Jul 7;13(7). Epub 2022 Jul 7.

College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.

Heat stress seriously affects medicinal herbs' growth and yield. Rubisco accumulation factor (Raf) is a key mediator regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which plays important roles in carbon assimilation and the Calvin cycle in plants. Raf has been studied in many plants, but has rarely been studied in the important medicinal plant fragrant woodfern (). The aim of this study was to analyze the effects of Raf on carbohydrate metabolism and the response to heat stress in medicinal plants. In this study, high temperature treatment upregulated the expression of , which was significantly higher than that of phosphoribokinase (), Rubisco small subunits (), Rubisco large subunits () and Rubisco activase (). The subcellular localization showed that the DfRaf proteins were primarily located in the nucleus; DfPRK, DfRbcS, DfRbcL and DfRCA proteins were primarily located in the chloroplast. We found that overexpression of led to increased activity of Rubisco, RCA and PRK under high-temperature stress. The HO, O and MDA content of the DfRaf-OV-L2 and DfRaf-OV-L6 transgenic lines were significantly lower than those of WT and VC plants under high-temperature stress. The photosynthetic pigments, proline, soluble sugar content and ROS-scavenging ability of the DfRaf-OV-L2 and DfRaf-OV-L6 transgenic lines were higher than those of WT and VC plants under high-temperature stress. The results showed that overexpression of the gene increased the Rubisco activity, which enhanced the high-temperature tolerance of plants.
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http://dx.doi.org/10.3390/genes13071212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321628PMC
July 2022

Heritability of Corneal Parameters in Nuclear Families With Keratoconus.

Transl Vis Sci Technol 2022 Jul;11(7):13

Henan University People's Hospital, Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, Zhengzhou 450003, China.

Purpose: This study aimed to investigate the heritability of corneal parameters obtained by Pentacam in nuclear families with keratoconus (KC).

Methods: A total of 82 patients with KC and their biological parents (n = 164) were recruited in the current study. All subjects underwent corneal tomography with Pentacam. Family units were analyzed to calculate the heritability of corneal parameters by linear mixed effects model using the R statistical software.

Results: The pachymetry at apex, pupil, and thinnest point were all significantly heritable at 43.26%, 42.63%, and 43.09%, respectively. The heritability of flat meridian keratometry, steep meridian keratometry, and mean keratometry in the anterior surface were 10.36%, 9.05%, and 10.21%, respectively, and that of flat meridian keratometry, steep meridian keratometry, and mean keratometry in the posterior surface were 8.44%, 9.67%, and 9.06%, respectively. The posterior radius of curvature had higher heritability in comparison with anterior radius of curvature (19.16% vs. 14.37%). Moreover, among combined topometric indices, the heritability of index of vertical asymmetry was the highest (19.49%), and that of central keratoconus index was the lowest (6.64%).

Conclusions: The present study demonstrated a substantial heritability of corneal parameters in nuclear families with KC. The pachymetric indices are heritable and may be suitable as KC endophenotypes, suggesting a necessity to discover the genes associated with corneal thickness in KC.

Translational Relevance: The pachymetric indices are heritable and may be suitable as KC endophenotypes, indicating that the pachymetric indices might be a corneal characteristic to predict the occurrence of KC.
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http://dx.doi.org/10.1167/tvst.11.7.13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296886PMC
July 2022

A Bibliometric Analysis of 100 Most-Cited Articles on Corneal Cross-Linking.

Front Med (Lausanne) 2022 1;9:904077. Epub 2022 Jun 1.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Zhengzhou, China.

Background: Highly cited papers are expected to have high-quality data that significantly contribute to the body of knowledge. The study aimed to evaluate the characters of the 100 most-cited articles on corneal cross-linking (CXL) through a bibliometric analysis.

Materials And Methods: The Web of Science database was searched to identify papers published from 1950 to 2020. A bibliometric analysis of the top 100-cited articles was conducted in the current study. The citation differences between basic research, clinical research, and reviews were compared by Kruskal-Wallis test. The association between citations and publication year was evaluated by Spearman correlation analysis. The VOSviewer software was used to create networks of co-authorship and keywords map.

Results: The median values of the number of citations, citations/year since publication, and citations since 2013 were 101, 9.5, and 11.92, respectively. A total of 61% of articles were clinical research. The citations since 2013 of clinical research were lower than basic research and the reviews (all < 0.001). The publication year was positively correlated with the number of publications ( = 0.665, = 0.013), and the total number of citations decreased for basic research ( = -0.447, = 0.017), and clinical research ( = -0.433, < 0.001). The publishes the highest number of articles. The corresponding authors were predominantly from the Italy ( = 17), Germany ( = 16), and United States ( = 15). Spoerl Eberhard has the highest number of citations and total link strength with 15 articles. Extensive collaboration existed among the main core nodes containing "cross-linking ( = 45)," "riboflavin ( = 44)," and "ultraviolet A (UVA) ( = 42)."

Conclusion: The present study focused on the comprehensive analysis of the top 100-cited articles on the CXL research, providing insight into research developments over the past decades.
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http://dx.doi.org/10.3389/fmed.2022.904077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199002PMC
June 2022

Impact of lurasidone on health-related quality of life in adults with bipolar depression: a post-hoc analysis.

Curr Med Res Opin 2022 Jun 14:1-7. Epub 2022 Jun 14.

Sunovion Pharmaceuticals Inc, Marlborough, MA, USA.

Objective: The objective of this post-hoc analysis was to assess the impact of lurasidone monotherapy on health-related quality of life (HRQoL) in adults with bipolar depression.

Methods: Data were analyzed from a 6-week randomized, double-blind (DB), placebo-controlled trial of lurasidone monotherapy (NCT00868699) and a 6-month open label extension (OLE; NCT00868959). Patients who received lurasidone monotherapy or placebo during the DB trial were eligible to continue or switch to lurasidone monotherapy during the OLE. The 16-item Quality of Life Enjoyment and Satisfaction Questionnaire-Short Form (Q-LES-Q-SF) was collected at DB baseline, DB week 6/OLE baseline, OLE month 3, and OLE month 6. Effect size (ES) and mean changes from baseline were reported for Q-LES-Q-SF total and item scores during the DB trial and OLE, respectively.

Results: Of 485 patients in the DB trial (lurasidone monotherapy:  = 323; placebo:  = 162), 316 patients continued or switched to lurasidone monotherapy during the OLE. Significant improvements in Q-LES-Q-SF scores in lurasidone vs. placebo were reported for 13 of 16 items (all  < .05) at DB week 6. The greatest improvements were overall life satisfaction (ES = 0.57), social relationships (0.55), medication satisfaction (0.48), family relationships (0.46), and ability to function in daily life (0.45, all  < .001). Improvements in Q-LES-Q-SF total and item scores were sustained at OLE month 6.

Conclusions: Treatment with lurasidone provided a significant improvement across HRQoL items including overall life satisfaction, social and family relationships, medication satisfaction, and ability to function in daily life. Improvements were sustained during the 6-month OLE.
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http://dx.doi.org/10.1080/03007995.2022.2083400DOI Listing
June 2022

Protective effect of Glutaredoxin 1 against oxidative stress in lens epithelial cells of age-related nuclear cataracts.

Mol Vis 2022 19;28:70-82. Epub 2022 May 19.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Purpose: Glutaredoxin 1 (Grx1) is a key antioxidant protein that catalyzes disulfide redox reactions. In this study, we investigated the expression and protective effect of Grx1 against oxidative stress in nuclear cataracts.

Methods: Human anterior capsule membrane samples were obtained from the eyes of cataract patients (experimental group) and non-cataractous (control group) donors. The levels of Grx1 protein and mRNA expression were investigated. The human lens epithelial (HLE) cell line SRA 01/04 was transfected with Grx1-containing plasmid or Grx1 small interfering RNA, and cultured under HO treatment, mimicking oxidative stress conditions. Cell counts, clone formation, cell apoptosis, cell cycle, and levels of oxidized glutathione disulfide and cellular reactive oxygen species (ROS) were evaluated and quantified.

Results: Protein and mRNA transcript levels of Grx1 were significantly lower in the human anterior capsule membrane of the age-related nuclear (ARN) cataract group than in the control group. Grx1 overexpression protected HLE cells from HO-induced oxidative damage, including alleviating G1 phase arrest, promoting cell proliferation, reducing cell apoptosis, and decreasing intracellular ROS generation. Furthermore, extracellular-signal-regulated kinase (ERK) phosphorylation in the human anterior capsule membrane of ARN patients was higher in the experimental group than in the control group. Grx1 overexpression reduced the levels of oxidized glutathione disulfide and the phosphorylation of ERK. The administration of an ERK phosphorylation inhibitor, PD98059, induced antioxidant effects in Grx1-silenced cells.

Conclusions: Grx1 expression is downregulated in the human anterior capsule membrane of ARN patients, accompanied by an increase in ERK phosphorylation. Thus, Grx1 can protect HLE cells against oxidative stress.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122476PMC
May 2022

A Hospital-Based Study on the Prevalence of Keratoconus in First-Degree Relatives of Patients with Keratoconus in Central China.

J Ophthalmol 2022 26;2022:6609531. Epub 2022 May 26.

Henan University People's Hospital, Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, Zhengzhou 450003, China.

Purpose: The present study investigated the prevalence of keratoconus (KC) among first-degree relatives of KC patients in Central China.

Methods: From July 2018 to March 2022, 661 first-degree relatives of 384 KC patients were included in the present study. Corneal tomography, uncorrected distance visual acuity, manifest refraction with corrected distance visual acuity, intraocular pressure, slit-lamp microscopy, and fundus examination were performed. The diagnosis of KC was based on the Belin/Ambrosio enhanced ectasia total deviation value (BAD-D value) on the Pentacam® system (Oculus GmbH). BAD-D value <1.6 was diagnosed as healthy, 1.6≤ BAD-D value <2.6 was diagnosed as suspected KC, and BAD-D value ≥2.6 was diagnosed as KC.

Results: The present study included 337 (50.98%) female and 324 (49.02%) male subjects. The prevalence of KC and suspected KC in first-degree relatives was 8.77% ( = 58) and 29.05% ( = 192), respectively. The prevalence of KC was 9.70% among parents and 7.23% among siblings.

Conclusions: The higher prevalence of KC among first-degree relatives of patients with KC suggests that first-degree relatives of KC are at high risk of developing KC.
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http://dx.doi.org/10.1155/2022/6609531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162836PMC
May 2022

Water-Induced Self-Assembly and Mineralization within Plant Phenolic Glycol-Gel toward Ultrastrong and Multifunctional Thermal Insulating Aerogels.

ACS Nano 2022 Jun 2. Epub 2022 Jun 2.

Institute of Biomass Engineering, South China Agricultural University, Guangzhou 510642, China.

Biopolymer/silica nanocomposite aerogels are highly attractive as thermally insulating materials for prevailing energy-saving engineering but are usually plagued by their lack of mechanical strength and environmental stability. Lignin is an appealing plant phenolic biopolymer due to its natural abundance, high stiffness, water repellency, and thermostability. However, integrating lignin and silica into high-performance 3D hybrid aerogels remains a substantial challenge due to the unstable co-sol process. In diatoms, the silicic acid stabilization prior to the condensation reaction is enhanced by the intervention of biomolecules in noncovalent interactions. Inspired by this mechanism, we herein rationally design an ultrastrong silica-mineralized lignin nanocomposite aerogel (LigSi) with an adjustable multilevel micro/nanostructure and arbitrary machinability through an unusual water-induced self-assembly and mineralization based on ethylene glycol-stabilized lignin/siloxane colloid. The optimized LigSi exhibits an ultrahigh stiffness (a specific modulus of ∼376.3 kN m kg) and can support over 5000 times its own weight without obvious deformation. Moreover, the aerogel demonstrates a combination of outstanding properties, including superior and humidity-tolerant thermal insulation (maintained at ∼0.04 W m K under a relative humidity of 33-94%), excellent fire resistance withstanding an ∼1200 °C flame without disintegration, low near-infrared absorption (∼9%), and intrinsic self-cleaning/superhydrophobic performance (158° WCA). These advanced properties make it an ideal thermally insulating material for diversified applications in harsh environments. As a proof of concept, a dual-mode LigSi thermal device was designed to demonstrate the application prospect of combining passive heat-trapping and active heating in the building.
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http://dx.doi.org/10.1021/acsnano.2c00755DOI Listing
June 2022

Trinuclear anionic gold clusters bridged by dialkylsilylene and dialkylgermylene.

Chem Commun (Camb) 2022 Jun 9;58(47):6705-6708. Epub 2022 Jun 9.

College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, P. R. China.

While the chemistry of gold clusters has been developed rapidly, the knowledge of trinuclear gold clusters with low-valent gold remains scarce. Herein, we report the synthesis, structural characterization, and density functional theory (DFT) calculations of trinuclear anionic gold clusters 3a and 3b ligated by dialkylsilylene (1a) and dialkylgermylene (1b), respectively.
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http://dx.doi.org/10.1039/d2cc02054hDOI Listing
June 2022

A bibliometric analysis of the top 100 most-cited articles on keratoconus.

Int Ophthalmol 2022 May 12. Epub 2022 May 12.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, 7 Weiwu Road, Zhengzhou, 450003, Henan, People's Republic of China.

Purpose: To explore the characteristics of the top 100 most-cited articles on keratoconus research.

Methods: The Web of Science database was searched to identify papers published from 1950 to 2020. A bibliometric analysis of the top 100 most-cited articles was conducted in the current study. The Kruskal-Wallis test was conducted to test the citation differences between basic research, clinical research, and reviews. The Spearman correlation analysis was used to investigate the association between citations and publication year. The VOSviewer software was used to create networks of co-authorship and keywords map.

Results: The median values of the number of citations, citations/year since publication, and citations since 2013 were 186.5, 13.90, and 11, respectively. A total of 79% of articles were clinical research. Reviews exhibited a higher value of citations/year since publication and citations since 2013 than basic research (all P < 0.05). The number of publications, total number of citations, citations/year since publication and citations since 2013 on the top 100 most-cited articles list increased with an increase in the publication year in three article types (all P < 0.05). The Journal of Cataract and Refractive Surgery had the highest number of articles. There were 37 articles from the USA. Rabinowitz, YS was the most-cited author with the greatest total link strength. Extensive collaboration existed among the main core nodes containing "cross-linking," "refractive surgery," and "penetrating keratoplasty."

Conclusions: The present study focused on the comprehensive analysis of the top 100 most-cited articles on keratoconus research, providing insight into keratoconus research developments over the past decades.
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http://dx.doi.org/10.1007/s10792-022-02334-1DOI Listing
May 2022

Analysis of Psychological Changes and Intervention Mechanism of Elderly Groups Based on Deep Learning Analysis Technology.

Authors:
Yue Gao Qi Fan

Comput Intell Neurosci 2022 30;2022:1686219. Epub 2022 Apr 30.

Institute of Mental Health, Nanjing Xiaozhuang University, Nanjing 210017, China.

The elderly group is a unique social phenomenon in China. This study analyzes the typology of psychological changes in the elderly group based on the analysis of deep learning techniques and also combines crisis intervention theory to study the intervention strategies of social workers in different stages of emotional changes in the elderly group. A questionnaire survey of the elderly was conducted using the survey method, in which 10,948 valid questionnaires were screened from the Psychological Condition Self-check Scale and 11,040 valid questionnaires were screened from the Mental Health Survey Questionnaire for the Elderly. The degree of negative emotions of the elderly group in public emergencies was not related to age, but significantly correlated with age ( value < 0.05), and there was a tendency that the higher the age, the deeper the degree; in addition, elderly people of different professions also showed significant differences ( < 0.05); elderly people of different regions also showed significant differences ( < 0.05). In crisis intervention, social workers mainly provide services such as initial diagnosis, primary intervention, secondary intervention, and assessment for the caseworkers. The practical study found that social workers need to use strategies such as short-term focal solutions, avoiding guiding clients with their own values; crisis intervention programmes should be flexible, proactively helping clients to rebuild their support network, developing clients' self-solving skills, and implementing interprofessional teamwork and whole-person rehabilitation services.
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http://dx.doi.org/10.1155/2022/1686219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078781PMC
May 2022

Oxidation stability enhanced MXene-based porous materials derived from water-in-ionic liquid Pickering emulsions for wearable piezoresistive sensor and oil/water separation applications.

J Colloid Interface Sci 2022 Jul 19;618:311-321. Epub 2022 Mar 19.

Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, Jinan 250100, P. R. China. Electronic address:

Hypothesis: Stemming from their unique superiorities, TiC-MXenes have emerged as versatile 2D materials for a myriad of appealing applications. However, two crucial issues are detrimental to maximize the inherent properties of MXenes for further specific developments, i.e. restacking problem and environmental instability.

Experiments: Herein, we develop an effective strategy, constructing water-in-ionic liquid (W/IL) Pickering emulsions with further polymerization of the continuous phase, to fabricate oxidation stability enhanced TiC-MXene based porous materials. It is the first time to utilize a brand new platform between the immiscible IL and water for MXene nanosheets to assemble with guest species serving as building blocks for macromonoliths.

Findings: The prepared porous materials can provide elastic hollow-sphere structures derived from emulsion template, for wearable piezoresistive sensor with high sensitivity, excellent accuracy and favorable reproducibility. Intriguingly, ILs as dispersion and surface modification with polymeric ionic liquids (PILs) play indispensable roles in ameliorating oxidation stability of MXenes in porous materials, by virtue of quenching reactive oxygen species (ROS) and forming protective layer through the capping effect. Furthermore, the processed aerogels after supercritical drying can selectively absorb several organic solvents owing to their high hydrophobicity, abundant porosity and sufficient mechanical strength. All results indicate that the innovative strategy can simultaneously circumvent two major drawbacks of MXenes for the first time, and shed light on the opportunity to further enrich their practical applications by constructing multifunctional platform.
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http://dx.doi.org/10.1016/j.jcis.2022.03.073DOI Listing
July 2022

Species Diversity and Virulence Potential of the Complex and Complex.

Front Microbiol 2022 4;13:841604. Epub 2022 Mar 4.

Yunnan Herbal Laboratory, School of Ecology and Environmental Science, Yunnan University, Kunming, China.

is a very important fungal resource. Some species have great economic and ecological value. Through surveying in China and Thailand over the past 4 years, 15 spp. were collected and identified. Three new species-namely, , , and -were described and illustrated based on morphological characteristics and molecular data. The phylogenetic positions of the 15 species were evaluated according to phylogenetic inferences based on six loci (nr, nr, , , , and ). Nine species of in our study were isolated from adult scarab beetles (Coleoptera: Scarabaeidae). The pathogenicity of the isolates from the complex and complex was determined with three bioassays using and larvae, in addition to adults. The results indicated that the complex isolates had great potential in the biocontrol of the three insects; by contrast, the complex isolates showed obvious host specificity and low virulence.
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http://dx.doi.org/10.3389/fmicb.2022.841604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934399PMC
March 2022

SIK2 maintains breast cancer stemness by phosphorylating LRP6 and activating Wnt/β-catenin signaling.

Oncogene 2022 04 11;41(16):2390-2403. Epub 2022 Mar 11.

Xiangya Cancer Center, Xiangya Hospital, Central South University, Changsha, 410008, China.

Breast cancer stem cells (BCSCs) are the main drivers of recurrence and metastasis. However, commonly used drugs rarely target BCSCs. Via screenings, we found that Salt-inducible kinase 2 (SIK2) participated in breast cancer (BC) stemness maintenance and zebrafish embryos development. SIK2 was upregulated in recurrence samples. Knockdown of SIK2 expression reduced the proportion of BCSCs and the tumor initiation of BC cells. Mechanistically, SIK2, phosphorylated by CK1α, directly phosphorylated LRP6 in a SIK2 kinase activity-dependent manner, leading to Wnt/β-catenin signaling pathway activation. ARN-3236 and HG-9-91-01, inhibitors of SIK2, inhibited LRP6 phosphorylation and β-catenin accumulation and disturbed stemness maintenance. In addition, the SIK2-activated Wnt/β-catenin signaling led to induction of IDH1 expression, causing metabolic reprogramming in BC cells. These findings demonstrate a novel mechanism whereby Wnt/β-catenin signaling pathway is regulated by different kinases in response to metabolic requirement of CSCs, and suggest that SIK2 inhibition may potentially be a strategy for eliminating BCSCs.
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http://dx.doi.org/10.1038/s41388-022-02259-0DOI Listing
April 2022

Trends in research on corneal cross linking from 2001 to 2020: a bibliometric analysis.

Clin Exp Optom 2022 Feb 20:1-7. Epub 2022 Feb 20.

Xinxiang Medical University, Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, Zhengzhou, China.

Clinical Relevance: The research status and hotspots in the field of corneal cross linking (CXL) can benefit clinicians, researchers and the general public.

Background: The purpose of this study was to map the publishing trend on CXL research and explore the research hotspots.

Methods: A bibliometric analysis was performed using the Web of Science Core Collection to investigate the publishing trend on CXL research. VOSviewer was used to build the knowledge map to visualise the number of annual publications, distribution of countries and institutions, international cooperation, author productivity, source journals and research hotspots in the field of CXL.

Results: A total of 2061 peer-reviewed articles on CXL research were collected from 2001 to 2020, and the annual research production increased over time. The United States was the country with the largest number of published articles, and the University of Zurich was the most active institution. Hafezi F published the largest number of articles on CXL, while was the journal with the largest number of studies on CXL. The most frequently cited references mainly focus on CXL in the treatment of keratoconus. The keywords were divided in 5 categories: 1) CXL mechanism, 2) ectasia diseases and refractive surgery, 3) corneal biomechanics, 4) efficacy evaluation, 5) treatment of infectious keratitis.

Conclusion: The quantity and quality of articles on CXL were evaluated using bibliometric techniques by extracting the data from the Web of Science Core Collection. The research hotspots could provide insights on CXL research, providing valuable information for clinicians to perform research in this field and find potential partners.
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http://dx.doi.org/10.1080/08164622.2022.2038013DOI Listing
February 2022

Effect of acupuncture therapy combined with fluticasone propionate in the treatment of persistent allergic rhinitis: study protocol for a randomized controlled trial.

Trials 2022 Jan 31;23(1):94. Epub 2022 Jan 31.

Luohu District Hospital of Traditional Chinese Medicine, ShenZhen, 518004, China.

Background: Allergic rhinitis (AR) is an immunoglobulin E (IgE)-mediated inflammatory response. Persistent allergic rhinitis (PAR) is a subtype of AR, but the treatment of PAR is still a problem. Acupuncture is used as an alternative therapy for AR in clinical practice. The aim of this study is to evaluate the effectiveness of acupuncture therapy combined with fluticasone propionate nasal spray in comparison to fluticasone propionate nasal spray alone in the relief of symptoms for PAR.

Methods: This study is a multicenter, single-blind, randomized controlled trial. A total of 260 eligible patients will be randomly assigned into the treatment group or the control group. The treatment group will receive the nasal fluticasone propionate combined with acupuncture, and the control group will receive fluticasone propionate nasal spray alone for 6 weeks. The primary outcome is the change in the Reflective Total Nasal Symptom Score (rTNSS) from baseline to the end of treatment, and the Total Non Nasal Symptom Score (TNNSS), reflective total ocular symptom score (rTOSS), Rhinitis Quality of Life Questionnaire (RQLQ), use of antiallergic drugs, and the Rhinitis Control Assessment Test (RCAT) are used as secondary outcomes. The participants will be followed up for another 24 weeks after treatment.

Discussion: This clinical trial will be able to provide high level evidence on the acupuncture therapy combined with fluticasone propionate nasal spray in the treatment of PAR.

Trial Registration: ISRCTN Registry, ID: ISRCTN44040506 . Registered on 22 July 2020.
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http://dx.doi.org/10.1186/s13063-022-06020-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802421PMC
January 2022

Dual-specificity Tyrosine Phosphorylation-regulated Kinase Inhibitor ID-8 Promotes Human Somatic Cell Reprogramming by Activating PDK4 Expression.

Stem Cell Rev Rep 2022 Jan 26. Epub 2022 Jan 26.

Fujian Provincial Key Laboratory of Neurodegenerative, Disease and Aging Research, Institute of Neuroscience, School of Medicine, Xiamen University, Xiamen, 361005, Fujian, China.

Background: Human induced pluripotent stem cells (hiPSCs) hold great potentials in disease modeling, drug screening and cell therapy. However, efficiency and costs of hiPSCs preparation still need to be improved.

Methods: We screened the compounds that target signaling pathways, epigenetic modifications or metabolic-process regulation to replace the growth factors. After small molecule treatment, TRA-1-60, which is a cell surface antigen expressed by human embryonic stem cells (hESCs), staining was performed to quantify the efficiency of somatic cell reprogramming. Next, small molecule cocktail-induced ESCs or iPSCs were examined with pluripotent markers expression. Finally, Genome-wide gene expression profile was analyzed by RNA-seq to illustrate the mechanism of human somatic cell reprogramming.

Result: Here, we found that a dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of pyruvate dehydrogenase kinase 4 (PDK4) and activation of glycolysis. Furthermore, we identified a novel growth-factor-free hiPSC generation system using small molecules ID-8 (I) and TGFβ signal pathway agonist Kartogenin (K). Importantly, we developed IK medium combined with low-dose bFGF to support the long-term expansion of human pluripotent stem cells. IK-iPSCs showed pluripotency and normal karyotype.

Conclusions: Our studies may provide a novel growth-factor-free culture system to facilitate the generation of hiPSCs for multiple applications in regenerative medicine. In Brief Xu et at. found that a dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of PDK4 and activation of glycolysis. Furthermore, we established a novel growth-factor-free hiPSC generation system using small molecules ID-8/Kartogenin (IK). IK medium combined with Low-dose bFGF (IKB medium) supported the long-term expansion of human pluripotent stem cells. Highlights ID-8 Enhanced Reprogramming of Human Fibroblasts and Astrocytes Establishment of the Growth-factor-free Reprogramming System Using Small Molecule Compounds IK IKB Medium Maintained the Long-term Expansion of Human Pluripotent Stem Cells ID-8 Promoted Human Somatic Cell Reprogramming by Activating PDK4 Expression.
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http://dx.doi.org/10.1007/s12015-021-10294-9DOI Listing
January 2022

Monophasic and Biphasic Hyaluronic Acid Fillers for Esthetic Correction of Nasolabial Folds: A Meta-Analysis of Randomized Controlled Trials.

Aesthetic Plast Surg 2022 Jan 23. Epub 2022 Jan 23.

Medical Affairs Department, Tigermed-Jyton, Shanghai, People's Republic of China.

Background: Hyaluronic acid (HA) fillers have become the most popular dermal fillers for esthetic nasolabial folds (NLFs) correction. Based on the cross-linking method, they can be classified as monophasic or biphasic types.

Objectives: The aim of this meta-analysis is to compare the efficacy and safety of monophasic HA fillers with that of biphasic HA fillers for treatment of NLFs. PubMed, Embase, Cochrane Library, Web of Science, ClinicalTrials.gov, and Chinese databases were searched from inception to January 2021.

Methods: Randomized controlled trials (RCTs) that compared treatment outcomes of a monophasic HA filler with a biphasic HA filler for esthetic NLF correction were selected. Twenty-two RCTs with totally 1848 subjects were included to this meta-analysis. A significantly higher improvement of NLF severity score was seen for monophasic fillers than biphasic fillers at 2 (p = 0.005), 8 (p <0.0001), 12 (p = 0.03), 16 (p = 0.005), 24 (p <0.00001), 36 (p = 0.007), and 52 week (p <0.0001) after treatment.

Results: The effect was retained regardless of ethnicity, total injection volume, and baseline NLF severity. More subjects preferred monophasic fillers over biphasic ones (≤7 months: RR = 3.45, 95% CI 1.17 to 10.11; >7 months: RR = 2.37, 95% CI 1.45 to 3.86). Rate of post-injection pain was significantly lower for monophasic fillers (RR = 0.85, 95% CI 0.78 to 0.92, p <0.0001), but no statistical difference was observed for other post-injection adverse events. Compared to biphasic HA fillers, monophasic HA fillers were associated with a greater and more durable esthetic NLFs improvement, a lower rate of post-injection pain, and were favored by more patients.

Level Of Evidence Ii: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02729-yDOI Listing
January 2022

Clinical Characteristics and Early Visual Outcomes of Highly Myopic Cataract Eyes: The Shanghai High Myopia Study.

Front Med (Lausanne) 2021 4;8:671521. Epub 2022 Jan 4.

Department of Ophthalmology, Eye Institute, Eye & ENT Hospital, Fudan University, Shanghai, China.

To report ocular characteristics and early visual outcomes of highly myopic cataract eyes, and to analyze the risk factors of low vision. A total of 2,027 eyes of 1,400 cataract patients with axial length (AL) ≥ 26 mm undergoing cataract surgery in Eye & ENT Hospital of Fudan University, who were registered in the Shanghai High Myopia Study, were analyzed. Routine pre-operative ophthalmic examinations were performed and macular scan of optical coherence tomography (OCT) were obtained. Macular complications, central foveal thickness (CFT) and subfoveal choroidal thickness (SFCT) were evaluated from OCT images. Ocular and surgical history and perioperative complications were also recorded. Uncorrected and best-corrected visual acuity (UCVA/BCVA) 1 month post-operatively and its influencing factors were evaluated. The average AL of all involved eyes was 29.52 ± 2.26 mm, and 39.7% of which were with an AL > 30 mm and 26.4% of which were with a corneal astigmatism more than 1.5 D. Nuclear cataract accounted for the largest proportion (70.6%). The rate of overall macular complications was 27.6%. Postoperative UCVA and BCVA were 0.70 ± 0.46 and 0.25 ± 0.32 logMAR, respectively. BCVA improved significantly after surgery (vs. < 0.001) and affected by the elongation of AL ( < 0.001) and thinning of CFT and SFCT (both < 0.001). The risk factors of post-operative low vision (BCVA < 20/66) were macular atrophy, lamellar macular hole, high corneal astigmatism, long AL, thin SFCT and junior surgeons, odds ratios ranging from 1.54 to 54.87 (all < 0.05). Cataract surgery could improve the VA of highly myopic eyes. Eye with macular complications, higher corneal astigmatism, longer AL, thinner SFCT, and who was treated by a junior surgeon, may have a high risk of low vision after surgery.
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http://dx.doi.org/10.3389/fmed.2021.671521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764297PMC
January 2022

New Target of Oxidative Stress Regulation in Cochleae: Alternative Splicing of the p62/Sqstm1 Gene.

J Mol Neurosci 2022 Apr 19;72(4):830-840. Epub 2022 Jan 19.

Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

We investigated oxidative stress and antioxidant response in the p62/Sqstm1-Keap1-Nrf2 pathway in C57BL/6 mice cochleae during age-related hearing loss (ARHL) and noise-induced hearing loss (NIHL), and the function of full-length and variant p62 in the regulation of Nrf2 activation. Groups of young (2 months), old (13-14 months), control, and acoustic trauma (AT) mice were examined cochlear damage and oxidative stress as follows: auditory brainstem response and hair cell counts; malondialdehyde (MDA) levels measured by assay kit and 7,8-dihydro-8-oxoguanine (8-oxoG) detected by immunohistochemistry. Full-length and variant p62 were examined for expression in cochleae, hippocampus (HIP), and auditory cortex (AC) using immunoblotting. Keap1-Nrf2 pathway activation was based on immunoblotting of nuclear Nrf2 and quantitative real-time PCR of Nrf2 target genes HO-1/NQO-1. The oxidative function of full-length and variant p62 was examined in HEI-OC-1 cells by flow cytometry. The results showed hearing loss, and cochlear hair cell loss was associated with MDA accumulation and 8-oxoG expression during ARHL and NIHL. Nrf2 showed no obvious changes in nuclear protein. Expression levels mRNA for HO-1 and NQO1 were lower in old mice and mildly greater in AT Mice. The expression of p62 splicing variant lacking the Keap1-interacting region was greater than full-length p62 in cochleae. However, the expression of p62 splicing variant was lesser than full-length p62 in HIP and AC. For HEI-OC-1 cells, overexpression of full-length p62 decreased ROS levels induced by HO. Oxidative stress is closely related to ARHL and NIHL. Changing the ratio of full-length to variant p62 protein expression may be a new target to reduce the level of oxidative stress in cochleae.
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http://dx.doi.org/10.1007/s12031-022-01969-0DOI Listing
April 2022

New Target of Oxidative Stress Regulation in Cochleae: Alternative Splicing of the p62/Sqstm1 Gene.

J Mol Neurosci 2022 Apr 19;72(4):830-840. Epub 2022 Jan 19.

Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

We investigated oxidative stress and antioxidant response in the p62/Sqstm1-Keap1-Nrf2 pathway in C57BL/6 mice cochleae during age-related hearing loss (ARHL) and noise-induced hearing loss (NIHL), and the function of full-length and variant p62 in the regulation of Nrf2 activation. Groups of young (2 months), old (13-14 months), control, and acoustic trauma (AT) mice were examined cochlear damage and oxidative stress as follows: auditory brainstem response and hair cell counts; malondialdehyde (MDA) levels measured by assay kit and 7,8-dihydro-8-oxoguanine (8-oxoG) detected by immunohistochemistry. Full-length and variant p62 were examined for expression in cochleae, hippocampus (HIP), and auditory cortex (AC) using immunoblotting. Keap1-Nrf2 pathway activation was based on immunoblotting of nuclear Nrf2 and quantitative real-time PCR of Nrf2 target genes HO-1/NQO-1. The oxidative function of full-length and variant p62 was examined in HEI-OC-1 cells by flow cytometry. The results showed hearing loss, and cochlear hair cell loss was associated with MDA accumulation and 8-oxoG expression during ARHL and NIHL. Nrf2 showed no obvious changes in nuclear protein. Expression levels mRNA for HO-1 and NQO1 were lower in old mice and mildly greater in AT Mice. The expression of p62 splicing variant lacking the Keap1-interacting region was greater than full-length p62 in cochleae. However, the expression of p62 splicing variant was lesser than full-length p62 in HIP and AC. For HEI-OC-1 cells, overexpression of full-length p62 decreased ROS levels induced by HO. Oxidative stress is closely related to ARHL and NIHL. Changing the ratio of full-length to variant p62 protein expression may be a new target to reduce the level of oxidative stress in cochleae.
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http://dx.doi.org/10.1007/s12031-022-01969-0DOI Listing
April 2022

The impact of lurasidone on functioning and indirect costs in adults with bipolar depression: a post-hoc analysis.

J Med Econ 2022 Jan-Dec;25(1):152-159

Sunovion Pharmaceuticals Inc., Marlborough, MA, USA.

Objective: The aim of this post-hoc analysis was to assess the impact of lurasidone monotherapy on functional impairment, productivity, and associated indirect costs in patients with bipolar depression.

Methods: Data were analyzed from a 6-week randomized, double-blind (DB; NCT00868699), placebo-controlled trial of lurasidone monotherapy and a 6-month open label extension (OLE; NCT00868959) study. Patients with bipolar depression who completed the 6-week DB trial were subsequently enrolled in the OLE. Analysis of the OLE was limited to patients who either continued lurasidone (LUR-LUR) or switched from placebo to lurasidone monotherapy (PBO-LUR). The Sheehan Disability Scale (SDS), which measures functional impairment and productivity, was collected at DB baseline, DB week 6/OLE baseline, OLE month 3, and OLE month 6. Annual indirect costs were calculated based on days lost or unproductive from work/school due to symptoms. Effect sizes (ES) in functioning and days lost/unproductive were reported for the DB trial and mean changes for the OLE.

Results: A total of 485 patients were enrolled in the DB trial (lurasidone:  = 323; placebo:  = 162) and 316 were in the lurasidone monotherapy group during the OLE (LUR-LUR:  = 210; PBO-LUR:  = 106). In the DB trial, improvements in functioning (work: ES = 0.36,  = .0071; social: ES = 0.55,  < .0001; family: ES = 0.50,  < .0001) were significantly greater for lurasidone compared to placebo. Reductions in days lost (ES = 0.33,  = .0050) and unproductive (ES = 0.45,  = .0001) were significantly higher for lurasidone vs. placebo. This resulted in a greater reduction in indirect costs for lurasidone vs. placebo (least squares mean (standard error) = -$32,322 ($2,100) vs. -$20,091 ($2,838)). Improvements in functioning and productivity were sustained during the 6-month OLE for both LUR-LUR and PBO-LUR.

Conclusions: Lurasidone monotherapy for the treatment of bipolar depression significantly improved functioning and reduced indirect costs vs. placebo at week 6. Significant improvements in functioning and productivity were sustained for 6 months for both LUR-LUR and PBO-LUR.
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http://dx.doi.org/10.1080/13696998.2022.2030147DOI Listing
February 2022

Healthcare resource utilization and quality of life by cognitive impairment in patients with schizophrenia.

Schizophr Res Cogn 2022 Jun 21;28:100233. Epub 2021 Dec 21.

Sunovion Pharmaceuticals Inc., Marlborough, MA, USA.

Objective: The objective was to investigate the association between cognitive impairment and healthcare resource utilization (HCRU) and quality of life (QoL) among patients with schizophrenia.

Methods: Data from the Adelphi Schizophrenia Disease Specific Programme™, a point-in-time survey of physicians and their patients, were collected in the United States between July-October 2019. Psychiatrists reported on patient cognitive function, HCRU, housing circumstances and employment status for their next 10 consulting adult patients with schizophrenia. Patients were classified as having no/mild or moderate/severe cognitive impairment and asked to complete a QoL questionnaire voluntarily. Multiple regression analysis estimated the association between severity of cognitive impairment and patient outcomes adjusting for patient demographics and clinical characteristics.

Results: Psychiatrists (n=124) reported on 651 and 484 patients with no/mild and moderate/severe cognitive impairment, respectively. Moderate/severe vs. no/mild cognitive impairment was associated with greater odds of hospitalization related to schizophrenia relapse within the last 12 months (adjusted odds ratio [aOR] [95% CI] = 2.23 [1.53-3.24]) and being unemployed due to disability (aOR = 2.39 [1.65-3.45]). Patients with moderate/severe vs. no/mild cognitive impairment had worse average QoL (EuroQoL 5-dimension [EQ-5D] Health Index: difference = -0.09 [-0.13 to -0.04]; EQ-5D Visual Analogue Scale: difference = -7.0 [-13.0 to -1.0]) and overall life satisfaction (Quality of Life Enjoyment and Satisfaction Questionnaire-Short Form: difference = -8.4 [-14.1 to -2.8]).

Conclusions: Moderate/severe cognitive impairment among patients with schizophrenia was associated with worse patient outcomes including greater risk of hospitalizations related to schizophrenia relapse. Treatment to improve cognitive function could benefit the large proportion of patients with schizophrenia who suffer from cognitive impairment.
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http://dx.doi.org/10.1016/j.scog.2021.100233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715204PMC
June 2022

Primary scleral-fixated posterior chamber intraocular lenses in patients with congenital lens subluxation.

BMC Ophthalmol 2021 Nov 29;21(1):411. Epub 2021 Nov 29.

Department of Ophthalmology, Eye & ENT Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China.

Objective: We investigated the long-term visual outcomes and ocular complications of primary scleral-fixated posterior chamber intraocular lenses (SF-PC-IOLs) in patients with congenital lens subluxation.

Methods: We enrolled 53 patients (77 eyes) with congenital lens subluxation caused by ectopia lentis, Marfan syndrome, and Weill-Marchesani syndrome who underwent primary implantation of a SF-PC-IOL. All patients underwent a complete ophthalmic examination include visual acuity (VA), intraocular pressure (IOP), intraocular lenses (IOL) position, intraoperative complications and postoperative complications. Cox regression analysis and survival analysis were used to evaluate the risk factors for postoperative complications.

Results: Seventy seven eyes from 53 patients were included. Mean age at surgery was 23 ± 20 years (5 to 67 years), with a mean follow-up of 39 ± 27 months (12 to 130 months). The best-corrected VA improved from 0.84 ± 0.55 to 0.26 ± 0.43 logarithms of the minimum angle of resolution (p < 0.001). Best-corrected VA improved postoperatively in 73 eyes (94%). The main causes of reduced vision after surgery were retinal pathologies and amblyopia. Complications included transient intraocular haemorrhage (2 eyes, 2.6%), early vitreous incarceration (2 eyes, 2.6%), retinal detachment (6 eyes, 7.8%) and IOL dislocation (3 eyes, 3.9%). Cox regression showed that postoperative eye trauma was a risk factor for long-term postoperative complications.

Conclusion: SF-PC-IOLs provide good visual outcomes in patients with congenital lens subluxation. The SF-PC-IOLs showed good stability, except in patients suffering from postsurgical eye trauma.
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http://dx.doi.org/10.1186/s12886-021-02182-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630913PMC
November 2021

Point-in-time survey of healthcare resource utilization, employment, quality of life and caregiver status by disease severity in patients with schizophrenia in the US.

Curr Med Res Opin 2022 Mar 3;38(3):469-478. Epub 2021 Dec 3.

Sunovion Pharmaceuticals Inc., Marlborough, MA, USA.

Objective: The objective of this survey was to assess patient outcomes and caregiver status by disease severity among patients with schizophrenia in the United States.

Methods: A point-in-time survey was conducted between July and October 2019 via the Adelphi Schizophrenia Disease Specific Programme. Psychiatrists reported on their next 10 eligible patients with schizophrenia including demographics, disease severity, treatment history and hospitalizations. Patients receiving treatment for schizophrenia were classified as mild, moderate or severe based on disease severity. Regression models adjusted for age, sex and race/ethnicity.

Results: Psychiatrists ( = 124) reported on 435 mild, 401 moderate and 247 severe patients. Greater severity of schizophrenia was associated with a greater number of hospitalizations related to schizophrenia relapse in the previous 12 months (moderate vs. mild: adjusted incidence rate ratio (aIRR) [95% CI] = 2.17 [1.60-2.94]; severe vs. mild: aIRR = 5.45 [3.59-8.27]), lower full-time employment (moderate vs. mild: adjusted odds ratio (aOR) = 0.15 [0.08-0.28]; severe vs. mild: aOR = 0.02 [0.002-0.12]) and greater unemployment due to disability (moderate vs. mild: aOR = 4.24 [3.02-5.97]; severe vs. mild: aOR = 10.85 [6.85-17.17]). Patients with severe vs. mild schizophrenia had lower average quality of life (QoL) measured by the EuroQoL 5-dimension Health Index (difference = -0.16 [-0.23-0.09]). Among patients requiring care, patients with severe vs. mild schizophrenia received more caregiver hours per week (aIRR = 1.89 [1.25-2.84]).

Conclusions: Greater severity of schizophrenia was associated with a significantly greater number of hospitalizations and greater unemployment due to disability. Compared with mild schizophrenia, severe schizophrenia was associated with worse patient QoL and greater caregiver hours.
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http://dx.doi.org/10.1080/03007995.2021.2007690DOI Listing
March 2022

The Protein Kinase Activity of NME7 Activates Wnt/β-Catenin Signaling to Promote One-Carbon Metabolism in Hepatocellular Carcinoma.

Cancer Res 2022 01 11;82(1):60-74. Epub 2021 Nov 11.

Department of Oncology, Xiangya Cancer Center, Xiangya Hospital, Central South University, Changsha, China.

Metabolic reprogramming by oncogenic signaling is a hallmark of cancer. Hyperactivation of Wnt/β-catenin signaling has been reported in hepatocellular carcinoma (HCC). However, the mechanisms inducing hyperactivation of Wnt/β-catenin signaling and strategies for targeting this pathway are incompletely understood. In this study, we find nucleoside diphosphate kinase 7 (NME7) to be a positive regulator of Wnt/β-catenin signaling. Upregulation of NME7 positively correlated with the clinical features of HCC. Knockdown of NME7 inhibited HCC growth and , whereas overexpression of NME7 cooperated with c-Myc to drive tumorigenesis in a mouse model and to promote the growth of tumor-derived organoids. Mechanistically, NME7 bound and phosphorylated serine 9 of GSK3β to promote β-catenin activation. Furthermore, MTHFD2, the key enzyme in one-carbon metabolism, was a target gene of β-catenin and mediated the effects of NME7. Tumor-derived organoids with NME7 overexpression exhibited increased sensitivity to MTHFD2 inhibition. In addition, expression levels of NME7, β-catenin, and MTHFD2 correlated with each other and with poor prognosis in patients with HCC. Collectively, this study emphasizes the crucial roles of NME7 protein kinase activity in promoting Wnt/β-catenin signaling and one-carbon metabolism, suggesting NME7 and MTHFD2 as potential therapeutic targets for HCC. SIGNIFICANCE: The identification of NME7 as an activator of Wnt/β-catenin signaling and MTHFD2 expression in HCC reveals a mechanism regulating one-carbon metabolism and potential therapeutic strategies for treating this disease.
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http://dx.doi.org/10.1158/0008-5472.CAN-21-1020DOI Listing
January 2022
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