Publications by authors named "Qi Cheng"

439 Publications

PDE4D binds and interacts with YAP to cooperatively promote HCC progression.

Cancer Lett 2022 May 18:215749. Epub 2022 May 18.

Department of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Key Laboratory for Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan, China. Electronic address:

The role of cAMP in the development of hepatocellular carcinoma (HCC) is controversial and the biological function of cAMP-hydrolysing enzyme phosphodiesterase 4D (PDE4D) in HCC remains unclear. In this study, we observed markedly higher PDE4D expression in HCC patients with poor survival. PDE4D bound to yes-associated protein (YAP), and PDE4D expression positively correlated with YAP expression in HCC. Overexpression of PDE4D increased YAP dephosphorylation and activity and promoted HCC cell growth in vitro and in vivo, which was attenuated by the YAP inhibitor verteporfin. In contrast, silencing PDE4D reduced YAP expression and HCC cell growth. Notably, forced expression of YAP promoted PDE4D and YAP target gene expression and cell growth, which were abrogated by the PDE4D inhibitor roflumilast. Mechanistically, silencing of YAP caused PDE4D downregulation and HCC cell apoptosis via extracellular signal-regulated kinase (ERK) activation. Roflumilast activated cAMP-PKA signaling and induced cAMP-PKA-dependent YAP phosphorylation at serine 127, resulting in YAP degradation and suppression of HCC growth, which were reversed by the PKA inhibitor PKI. Additionally, transfection of the YAP-S127A mutant reversed roflumilast-mediated suppression of YAP and cell growth. Taken together, our findings indicate that PDE4D binds to and interacts with YAP to promote HCC progression. Targeting the PDE4D-YAP interaction with roflumilast may be an effective strategy for HCC treatment.
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http://dx.doi.org/10.1016/j.canlet.2022.215749DOI Listing
May 2022

Using Bacteria and Fungi as Plant Biostimulants for Sustainable Agricultural Production Systems.

Recent Pat Biotechnol 2022 May 13. Epub 2022 May 13.

College of Life Sciences, Hebei Agricultural University, Baoding, Hebei, 071000, China; Global Alliance of HeBAU-CLS&HeQiS for BioAl-Manufacturing, Baoding, Hebei 071000, China.

Different compounds with bioactive constitutents can be applied as biostimulants to increase plant growth and development under both normal and stressful conditions. Biostimulants utilization can be considered as a sustainable and beneficial nutritional crop management, and may decrease the negative impacts associated with excessive chemical fertilization. Google scholar (scholar.google.com), Science Direct (sicencedirect.com), CAB Direct (cabdirect.org), Springer Link (springerlink.com), Scopus (scopus.com), Web of Science (web of knowledge.com), Taylor and Francis (tandfonline.com), and Wiley Online Library (onlinelibrary.wiley.com) have been checked. The search was done to all manuscript sections according to terms "Glomus intraradices", "Trichoderma atroviride", "Trichoderma reesei", "Heteroconium chaetospira", "Artherobacter spp.", "Acinetobacter spp.", "Enterobacer spp.", "Pseudomonas spp.", "Ochrobactrum spp.", "Bacilus spp.", "Rhodococcus spp.", "Biostimulants", and "Plant growth promotion". On the basis of initial check, Titles and Abstracts have been reviwed on the basis of online literature, and then articles were read carefully. Withinin the framework of sustainable crop management, this review article was aimed to provide an overview of the application of the most common fungi and bacteria as plant biostimulants on various crops.
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http://dx.doi.org/10.2174/1872208316666220513093021DOI Listing
May 2022

The high efficient Sb(III) removal by cauliflower like amorphous nanoscale zero-valent iron (A-nZVI).

J Hazard Mater 2022 May 4;436:129056. Epub 2022 May 4.

Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, School of Metallurgy and Environment, Central South University, Changsha, Hunan 410083, PR China.

In this study, cauliflower like amorphous nanoscale zero-valent iron (A-nZVI) was prepared and its performance on the removal of Sb(III) was investigated and compared with that of nZVI. The results indicated that the removal of Sb(III) by nZVI and A-nZVI followed the pseudo-second-order kinetic model and Langmuir isotherm model, but the removal of Sb(III) by A-nZVI was more stable and its removal capacity (558.2 mg/g) is much higher than that of nZVI (91.3 mg/g). Moreover, the effects of initial Sb(III) concentration, initial pH and anions such as Cl, NO, SO, PO, and AsO were also investigated. A-nZVI showed extremely high selectivity towards Sb(III) in that 500 mg/L of AsO and PO shows little impact on its removal, while the removal of Sb(III) by nZVI was almost inhibited under the same condition. The combination of SEM-EDS, XPS, XRD and FTIR revealed the removal of Sb(III) by nZVI and A-nZVI were synergistic effects of oxidation and adsorption, but less Sb(III) (39.5%) was oxidized by A-nZVI. More γ-FeOOH and γ-FeO were formed at the surface of A-nZVI during the reaction. Both oxides have high affinity toward Sb(III), which might cause the higher removal capacity and selectivity for the removal of Sb(III) by A-nZVI. In conclusion, A-nZVI showed great potential for the remediation of Sb(III) in groundwater.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129056DOI Listing
May 2022

LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.

Cancer Lett 2022 May 10;540:215725. Epub 2022 May 10.

The State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences. Beijing, 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine. Beijing, 100101, China. Electronic address:

Cancer stem cells (CSCs) are a subpopulation of cancer cells that drive tumour progression and metastasis. Anti-CSC strategies represent new targets for cancer therapies. However, CSCs are highly plastic and heterogeneous, making validation and targeting difficult without bona fide markers that define their identity, especially in a clinical setting. Here, we report that a leucine-rich repeat containing G protein-coupled receptor 4 (LGR4) cooperates with CD44 and PrPc; the latter contributes significantly to metastatic capacity and defines the stemness characteristics of colorectal CSCs. CD44PrPcLGR4 cells effectively developed into organoids and, when transplanted, generated orthotopic and metastatic tumours. Importantly, targeting LGR4 and PrPc with lentiviral shRNAs inhibited organoid development and the growth of orthotopic tumours by inhibiting Wnt/β-catenin signalling. Thus, our study offers a novel therapeutic strategy that simultaneously targets CSC stemness and metastatic properties.
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http://dx.doi.org/10.1016/j.canlet.2022.215725DOI Listing
May 2022

Abnormal Large-Scale Neuronal Network in High Myopia.

Front Hum Neurosci 2022 15;16:870350. Epub 2022 Apr 15.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Aim: Resting state functional magnetic resonance imaging (rs-fMRI) was used to analyze changes in functional connectivity (FC) within various brain networks and functional network connectivity (FNC) among various brain regions in patients with high myopia (HM).

Methods: rs-fMRI was used to scan 82 patients with HM (HM group) and 59 healthy control volunteers (HC group) matched for age, sex, and education level. Fourteen resting state networks (RSNs) were extracted, of which 11 were positive. Then, the FCs and FNCs of RSNs in HM patients were examined by independent component analysis (ICA).

Results: Compared with the HC group, FC in visual network 1 (VN1), dorsal attention network (DAN), auditory network 2 (AN2), visual network 3 (VN3), and sensorimotor network (SMN) significantly increased in the HM group. FC in default mode network 1 (DMN1) significantly decreased. Furthermore, some brain regions in default mode network 2 (DMN2), default mode network 3 (DMN3), auditory network 1 (AN1), executive control network (ECN), and significance network (SN) increased while others decreased. FNC analysis also showed that the network connection between the default mode network (DMN) and cerebellar network (CER) was enhanced in the HM group.

Conclusion: Compared with HCs, HM patients showed neural activity dysfunction within and between specific brain networks, particularly in the DMN and CER. Thus, HM patients may have deficits in visual, cognitive, and motor balance functions.
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http://dx.doi.org/10.3389/fnhum.2022.870350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051506PMC
April 2022

Combined first-principles calculations and experimental study on the photocatalytic mechanism of natural dolomite.

RSC Adv 2021 Jul 12;11(39):24416-24423. Epub 2021 Jul 12.

School of Physics and Optoelectronic Engineering, Yangtze University 1 Nanhuan Road Jingzhou Hubei 434023 China

Mineral-based photocatalysts have received great attention due to their low cost. In this study, the photocatalytic activity of natural dolomite and its mechanism were investigated based on designed experiments and first-principles calculations. The kinetic study showed that natural dolomite showed notable photocatalytic activity for the degradation of target compounds including methylene blue, diphenhydramine, and tetracycline. The EPR analysis demonstrated that O ˙, ˙OH, and O were produced in the dolomite system under simulated sunlight irradiation. The first-principles calculations indicated that the isomorphous substitution of Fe for Mg in the dolomite lattice led to the impurity levels appearing in the forbidden band, which caused a significant decrease of the band gap from 5.02 to 1.63 eV. As a result, natural dolomite could act as a semiconductor photocatalyst in photochemical reactions due to the substitution of Mg by Fe. Under simulated sunlight irradiation, photogenerated electron-hole pairs in the natural dolomite were separated and transferred to the surface, and then formed reactive radicals through further reactions, thereby enhancing the degradation of target compounds. This research may contribute to the understanding of the photocatalytic activity of natural minerals.
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http://dx.doi.org/10.1039/d1ra04525cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036630PMC
July 2021

Topological photonic crystal biosensor with valley edge modes based on a silicon-on-insulator slab.

Opt Express 2022 Mar;30(7):10792-10801

In the development of integrated sensing, how to reduce losses and improve robustness has always been one of the key problems to be solved. The topological photonic crystal structure based on the quantum Hall effect has gradually attracted the attention of researchers due to its unique immune defect performance and anti-scattering performance. Here, we have successfully applied the valley photonic crystal structures to topologically manipulate the light within the band gap of 252 THz-317 THz in a silicon-on-insulator platform. We experimentally demonstrated that satisfactory transmission performance can be obtained using the valley-dependent topological edge states below light cone, even if there are structure defects such as lattice missing and lattice mistake near the interface between two kinds VPCs. Based on the features of topological protection, a triangular cavity consisting of three 10×a-length sides is proposed, and the Q factor value reaches 1.83×10 with little influence from defects. Finally, based on drying etching technology, a biosensor with cavity-coupled waveguide structure was prepared, and the RI sensitivity was 1228 nm/RIU.
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http://dx.doi.org/10.1364/OE.443907DOI Listing
March 2022

Relation Between New York Heart Association Functional Class and Remnant Cholesterol, and Non-high Density Lipoprotein Cholesterol in Coronary Heart Disease Patients With Type 2 Diabetes Mellitus.

Angiology 2022 Apr 24:33197221091315. Epub 2022 Apr 24.

58301Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Remnant cholesterol (RC) and non-high-density lipoprotein cholesterol (non-HDL-C) are associated with coronary heart disease (CHD) and type 2 diabetes mellitus (T2DM). However, the association between RC, non-HDL-C, and CHD patients with T2DM has not been comprehensively investigated. We analyzed the association between RC, non-HDL-C, and cardiac function in CHD patients with T2DM. Of the 22 022 CHD patients from six hospitals in Tianjin, 5373 (24.4%) patients with T2DM had higher levels of RC and non-HDL-C ( < .001) than those without T2DM. Among CHD patients with T2DM, RC and non-HDL-C were positively associated with New York Heart Association (NYHA) class Ⅱ [RC: odds ratio (OR), 1.74; 95% confidence interval (CI), 1.50-2.01; < .01; non-HDL-C: OR, 1.23; 95% CI, 1.15-1.31; < .01]. After adjusting for confounding factors, this association remained (RC: OR, 1.22; 95% CI, 1.03-1.45; < .05; non-HDL-C: OR, 1.09; 95% CI, 1.02-1.17; < .05). These findings provide evidence of an independent positive association between RC, non-HDL-C, and NYHA functional classes. More research is warranted to confirm these findings and determine the mechanisms involved.
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http://dx.doi.org/10.1177/00033197221091315DOI Listing
April 2022

Multisystem inflammatory syndrome in adults (MIS-A): case finding through systematic review of electronic medical records.

Clin Infect Dis 2022 Apr 20. Epub 2022 Apr 20.

COVID-19 Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, United States.

Background: Multisystem inflammatory syndrome in adults (MIS-A) is a severe condition temporally associated with SARS-CoV-2 infection.

Methods: In this retrospective cohort study, we applied the U.S. Centers for Disease Control and Prevention (CDC) case definition to identify diagnosed and undiagnosed MIS-A cases among adults discharged April 2020-January 2021 from four Atlanta, Georgia hospitals affiliated with a single medical center. Non-MIS-A COVID-19 hospitalizations were identified using International Classification of Diseases, Tenth Revision encounter code U07.1. We calculated the ratio of MIS-A to COVID-19 hospitalizations, compared demographic characteristics of the two cohorts, and described clinical characteristics of MIS-A patients.

Results: We identified 11 MIS-A cases, none of which were diagnosed by the treatment team, and 5,755 COVID-19 hospitalizations (ratio 1: 523). Compared with patients with COVID-19, patients with MIS-A were more likely to be younger than 50 years (72.7% vs. 26.1%, p < 0.01) and to be non-Hispanic Black persons (81.8% vs. 50.0%, p = 0.04). Ten patients with MIS-A (90.9%) had at least one underlying medical condition. Two MIS-A patients (18.2%) had a previous episode of laboratory-confirmed COVID-19, occurring 37 and 55 days prior to admission. All MIS-A patients developed left ventricular systolic dysfunction. None had documented mucocutaneous involvement. All required intensive care, all received systemic corticosteroids, eight (72.7%) required mechanical ventilation, two (18.2%) required mechanical cardiovascular circulatory support, and none received intravenous immunoglobulin. Two (18.2%) died or were discharged to hospice.

Conclusions: MIS-A is severe but likely underrecognized complication of SARS-CoV-2 infection. Improved recognition of MIS-A is needed to quantify its burden and identify populations at highest risk.
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http://dx.doi.org/10.1093/cid/ciac303DOI Listing
April 2022

Carbenoxolone ameliorates allergic airway inflammation through NF-κB/NLRP3 pathway in mice.

Biol Pharm Bull 2022 Apr 15. Epub 2022 Apr 15.

Department of Pediatrics, Shengjing Hospital of China Medical University.

Asthma is a respiratory disease characterized by heterogeneous chronic airway inflammation. Activation of NOD-like receptor pyrin (NLRP3) inflammasome is involved in the development of many pulmonary inflammatory diseases. The role and regulatory mechanism of carbenoxolone (CBX) in ovalbumin (OVA)-induced asthma models are not fully clear. Therefore, the study investigated whether CBX ameliorates airway inflammation and remodeling, as well as its mechanism in OVA induced-inflammation in mice. Wright-Giemsa staining was used to count inflammatory cells in bronchoalveolar lavage fluid (BALF). The level of inflammatory cells infiltration, mucus cell proliferation, and collagen deposition in lung tissue were separately assessed by hematoxylin and eosin, periodic acid-Schiff, and Masson trichrome staining, respectively. Airway resistance (AR) was measured by non-invasive airway system. Immunohistochemical assay was used to observe NLRP3 expression area. The expression of NF-κB, p-NF-κB, IκB-α, p-IκB-α, NLRP3, pro-caspase-1, caspase-1, and IL-1β in lung tissue were measured using quantitative real-time PCR or western blotting. Our results showed that CBX can significantly attenuate the leukocyte count and the percentage of eosinophils and neutrophils in the BALF, peribronchial inflammation, airway mucus secretion, collagen deposition area, and AR in OVA-induced airway inflammation. In addition, the expression of p-NF-κB, p-IκB-α, NLRP3 and related factors were dramatically alleviated after CBX treatment. These data suggest that CBX has a significant protective effect on allergic airway inflammation by suppressing the activation of NLRP3 inflammasome through NF-κB pathway in asthmatic mice.
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http://dx.doi.org/10.1248/bpb.b21-01100DOI Listing
April 2022

The Effects of Amino acids, Phenols and Protein Hydrolysates as Biostimulants on Sustainable Crop Production and Alleviate Stresses.

Recent Pat Biotechnol 2022 Apr 12. Epub 2022 Apr 12.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Biostimulants which contain substances or products including natural compounds, special formulation and microorganisms have gained a considerable attention as sustainable method for heavy metal detoxification, stimulate natural toxins, controlling diseases and pests, may increase both water and nutrient efficiency. Biostimulants are important products in modern agriculture which composed of different heterogenous classes of compounds with a broad spectrum of action to increase both qualitative and quantitative productions. Amino acids can be useful in stress defence, photosynthesis, increase nutrient uptake, pollination and fruit formation, precursors to hormones and growth parameters. Amino acids are considered as precursors and constituents of proteins, which are well-known for stimulation of cell growth. Because, they are the basic building blocks of proteins, amino acids are very important in plant growth, development and metabolite synthesis. One of the diverse, notable and the large group of secondary metabolites is phenolic compounds which have important function in regulation of the plants physiological activities, oxidation-reduction processes and photosynthesis. Protein hydrolysates contain amino acids and peptides which is one of the most important kinds of biostimulants. Protein hydrolysates have notable capability to increase crop performance, particularly under environmental stress conditions. This review article is aimed to introduce and found more about the roles of different types of biostimulants on plant growth and final yield production with considering sustainable agriculture.
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http://dx.doi.org/10.2174/1872208316666220412133749DOI Listing
April 2022

Entrepreneurship Education and Health-Stress Analysis of College Teachers and Students Using Backpropagation Neural Network Model.

Authors:
Leiming Fu Qi Cheng

Front Psychol 2022 17;13:783188. Epub 2022 Mar 17.

School of Marxism, Anhui University, Hefei, China.

The purpose is to solve the problem of college students' employment difficulties. It is the development trend of the times to master the basic psychological pressure state of students and analyze students' problems by using modern technology and science. First, based on Marxist theory, the theory of entrepreneurship education and the characteristics of teachers and students in colleges are expounded, and the principle and algorithms of Backpropagation Neural Network (BPNN) are introduced. Second, from the perspective of entrepreneurship education and mental health factors of college students, the sample set of the BPNN model is collected using a Questionnaire Survey (QS). Then, the sample set is normalized to analyze the current college entrepreneurship education and the health and stress of college students. The results show that the comprehensive BPNN output of entrepreneurship education is 0.726, indicating that entrepreneurship education in colleges is at a moderate level. The proposed BPNN model can perform better than the traditional prediction methods in predicting college students' mental health, and the data fitting results are satisfactory. Overall, college students' entrepreneurship education should be improved, and schools should take more incentives to help adjust college students' mentality.
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http://dx.doi.org/10.3389/fpsyg.2022.783188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969219PMC
March 2022

Methods for predicting single-cell miRNA in breast cancer.

Genomics 2022 Mar 29;114(3):110353. Epub 2022 Mar 29.

Institute of Intelligent System and Bioinformatics, College of Intelligent Systems Science and Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address:

It has been demonstrated that miRNAs are involved in many biological processes including cell proliferation and differentiation, apoptosis, and stress responses. Although single-cell RNA sequencing technology is prevailing nowadays, it still remains challenging in quantifying miRNA at the single-cell level. Herein, we present the computational methods to infer the single-cell miRNA expression level using its target gene abundances. Firstly, we developed an enrichment-based approach in estimating miRNA expression considering miRNA-mRNA regulation information and miRNA-mRNA correlation signal captured from existing TCGA datasets. Further efforts were made to infer the miRNA expression with machine learning models. The methods were applied to compare the accuracy and robustness with the simulated single-cell data. Finally, we applied the method in single-cell RNA-seq triple negative breast cancer (TNBC) patients to further discover miRNA marker at the single-cell level for the malignant cells. Our tool is available online at: https://github.com/ChengkuiZhao/Single-cell-miRNA-prediction.
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http://dx.doi.org/10.1016/j.ygeno.2022.110353DOI Listing
March 2022

Visible-Light-Mediated Three-Component Radical Iodosulfonylative Cyclization of Enynes.

Org Lett 2022 Apr 30;24(13):2515-2519. Epub 2022 Mar 30.

School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225009, China.

An efficient three-component radical iodosulfonylative cyclization of enynes is described. The visible-light irradiation of iodoform with sulfinates enables sulfonyl radical generation under catalyst- and oxidant-free conditions and triggers the radical addition, cyclization and iodination cascade reactions, giving various vinyl iodide containing sulfones in moderate to good yields.
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http://dx.doi.org/10.1021/acs.orglett.2c00655DOI Listing
April 2022

Long non-coding RNAs in systemic lupus erythematosus: New insights into disease pathogenesis and diagnosis.

Scand J Immunol 2022 Mar 22:e13167. Epub 2022 Mar 22.

Department of Rheumatology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Systemic lupus erythematosus (SLE) is a remarkable heterogeneous autoimmune disease that is sometimes hard to diagnose at the early stage and can lead to premature mortality. Long non-coding RNAs (lncRNAs) are a class of non-protein-coding RNAs greater than 200 nucleotides in length that can regulate gene expression in various human diseases, including SLE. Peripheral blood samples and renal tissue samples from SLE patients were used for study. Abnormally expressed lncRNAs in SLE have been shown to influence several signalling pathways, including the IFN-I, MAPK and WNT pathways. This can affect cellular phenotypes like cell activation, differentiation skewing, cytokine production and cell apoptosis. Many of the reported lncRNAs may be useful for diagnosing, evaluating progression and predicting potential organ damage in SLE patients. While numerous lncRNAs play important roles in SLE, more basic and clinical studies are warranted to clarify the function of these regulatory molecules and determine their diagnostic value.
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http://dx.doi.org/10.1111/sji.13167DOI Listing
March 2022

An Oxidant- and Catalyst-Free Electrooxidative Cross-Coupling Approach to Synthesize -Substituted Porphyrin Derivatives.

J Org Chem 2022 Apr 18;87(7):4742-4749. Epub 2022 Mar 18.

College of Pharmacy, Third Military of Medical University, Chongqing 400038, PR China.

The synthesis of porphyrin and chlorin derivatives has attracted significant attention due to their numerous applications. Herein, we report an environment friendly oxidant- and catalyst-free electrooxidative cross-coupling approach for multiple coupling reactions to synthesize C-N, C-O, and C-S substituted porphyrin and chlorin derivatives. For C-N cross-coupling reactions, diaminated porphyrins were obtained as the main products, while using 4-bromo-2,6-dimethyl aniline resulted in monoaminated product. Similarly, electrochemical catalysis of porphyrins with phenol and thiophene produced -disubstituted porphyrins in moderate yields under a smaller current. Chlorins were also applicable, and 20-substituted products were efficiently produced regioselectively. To the best of our knowledge, this work represents the first example of electrooxidative C-X cross-coupling of porphyrins and chlorins.
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http://dx.doi.org/10.1021/acs.joc.2c00031DOI Listing
April 2022

Serum proteome profiling reveals differentially expressed proteins between subjects with metabolically healthy obesity and nonalcoholic fatty liver disease.

J Proteomics 2022 May 11;260:104556. Epub 2022 Mar 11.

Core Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211166, China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD) is the leading risk factor for common chronic liver disease and is often regarded as a prevalent metabolic disorder tightly associated with obesity. However, the existence of metabolically healthy obesity (MHO) indicates that some important factors may participate in protecting individuals with MHO free of NAFLD, even with excessive adiposity. To explore factors independent of obesity that may be involved in the occurrence of NAFLD, we performed an iTRAQ-based proteomic study to identify proteins differentially expressed in serum between NAFLD and MHO subjects. Compared with the MHO group, ten proteins were upregulated and five were downregulated significantly in the NAFLD group. Gene Ontology analysis indicated significant changes in the immune response and triglyceride metabolism-related pathways between MHO and NAFLD. We further validated three candidates markedly dysregulated in NAFLD by Western blotting and ELISA, including two upregulated proteins (afamin and apolipoprotein H) and one downregulated protein (apolipoprotein C-1). Detection of serum apolipoprotein H levels in a large-scale cohort with MHO and different stages of NAFLD indicated that apolipoprotein H may be a potential blood biomarker for distinguishing NAFLD from MHO and an independent risk factor for predicting NAFLD.
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http://dx.doi.org/10.1016/j.jprot.2022.104556DOI Listing
May 2022

Association between triglyceride glucose index and carotid artery plaque in different glucose metabolic states in patients with coronary heart disease: a RCSCD-TCM study in China.

Cardiovasc Diabetol 2022 03 11;21(1):38. Epub 2022 Mar 11.

Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.

Background: The triglyceride glucose (TyG) index serves as a surrogate indicator of insulin resistance. However, there is limited evidence on the association between the TyG index and carotid artery plaque (CAP) in patients with coronary heart disease (CHD).

Methods: The 10,535 CHD patients were divided according to TyG index quartiles (Q1: TyG index < 8.52; Q2: 8.52 ≤ TyG index < 8.93; Q3: 8.93 ≤ TyG index ≤ 9.40; Q4: TyG index > 9.40). The presence or absence of CAP was determined by carotid ultrasonography. Logistic regression was used to analyze the relationship between the TyG index and CAP in CHD patients. The relationship between the TyG index and CAP in according to sex, age groups, and glucose metabolism states were also assessed.

Results: The baseline analysis showed that there were significant differences in related parameters among CHD patients divided into four groups according to the quartile of the TyG index. In the multi-adjusted modles, compared to Q1 of the TyG index, the odds ratios (OR) for Q4 of the TyG index for CAP were 1.37 (95% confidence interval [CI] 1.28-1.47) in CHD patients. The association between the TyG index and CAP in female (OR: 1.35; 95% CI 1.29-1.43) was higher than that in male (OR: 1.20; 95% CI 1.13-1.27). The OR value of middle-aged (≤ 60 years old) patients (OR: 1.34; 95% CI 1.26-1.42) was higher than that in elderly (> 60 years old) patients (OR: 1.16; 95% CI 1.11-1.22). In different glucose metabolism states, the TyG index of CHD patients was significantly related to the risk of CAP, with the highest OR value observed for diabetes (OR: 1.36; 95% CI 1.26-1.46).

Conclusions: The TyG index and CAP showed a significant association in CHD patients. This association between TyG index and CAP in CHD patients is higher in female than in male, and the association in middle-aged and elderly patients is higher than that in elderly patients. In the condition of DM, the association between TyG index and carotid artery plaque in CHD patients is higher.
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http://dx.doi.org/10.1186/s12933-022-01470-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917731PMC
March 2022

Metal-enhanced fluorescence of graphene oxide sheets.

Anal Bioanal Chem 2022 May 7;414(12):3625-3630. Epub 2022 Mar 7.

Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, People's Republic of China.

Graphene oxide (GO) is an excellent chemical tunable optical platform for imaging and sensing. The photoluminescence (PL) quantum yield of GO is relatively low, which limited the application of the intrinsic and tunable fluorescence from GO. Here, we report the first case of metal-enhanced fluorescence (MEF) of GO. A significant enhancement (~10-fold) in fluorescence intensity is observed from GO on the Ag substrate as compared to that on the glass. FL, Raman, and SEM images are used to investigate the MEF behavior and are coincident with each other. The influence of the metal particle size of Ag substrate is investigated. The fluorescence is also found to be responsive when adding different metal ions into GO solution. GO contacting directly with metal substrate exhibits strong MEF without quenching, which makes it possible to use GO sheets for three-dimension optical imaging and sensing.
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http://dx.doi.org/10.1007/s00216-022-04001-xDOI Listing
May 2022

A new approach for reducing pollutants level: a longitudinal cohort study of physical exercises in young people.

BMC Public Health 2022 02 3;22(1):223. Epub 2022 Feb 3.

Core Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211166, China.

Background: The present study aimed to evaluate the elimination of three common pollutants (dimethoate, benzo(a)pyrene (BaP) and bisphenol A (BPA) by different physical exercises and to assess the possible factors which could affect the pollutants elimination.

Methods: A total of 200 individuals who chose different kinds of exercises in accordance to their own wish were recruited. The levels of urinary pollutants were measured using β-glucuronidase hydrolysis followed by a high-performance liquid chromatography tandem mass spectrometry-based method.

Results: Totally, the levels of dimethoate, BaP and BPA were reduced after physical exercises. However, the elimination of BaP in male was higher than that in female but the elimination of BPA in female was higher than that in male. Multivariate logistic regression showed that the degree of heart rate (HR) change was a protective factor affecting the improvement effect of dimethoate, BaP and BPA while BMI (body mass index) was a risk factor. Nevertheless, sex was a risk factor affecting the improvement of dimethoate and BaP but had a lower efficacy on BPA improvement.

Conclusion: The present findings indicate that physical exercises can be considered as a novel approach to eliminate pollutants level in human body and can also give suggestions for choosing specific physical exercises to male and female individuals. Moreover, those who are with higher BMI need to lose weight before eliminating pollutant level through physical exercises.
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http://dx.doi.org/10.1186/s12889-022-12621-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812347PMC
February 2022

A Shift from Efficiency to Adaptability: Recent Progress in Biomimetic Interactive Soft Robotics in Wet Environments.

Adv Sci (Weinh) 2022 03 24;9(8):e2104347. Epub 2022 Jan 24.

College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen, 518000, China.

Research field of soft robotics develops exponentially since it opens up many imaginations, such as human-interactive robot, wearable robots, and transformable robots in unpredictable environments. Wet environments such as sea and in vivo represent dynamic and unstructured environments that adaptive soft robots can reach their potentials. Recent progresses in soft hybridized robotics performing tasks underwater herald a diversity of interactive soft robotics in wet environments. Here, the development of soft robots in wet environments is reviewed. The authors recapitulate biomimetic inspirations, recent advances in soft matter materials, representative fabrication techniques, system integration, and exemplary functions for underwater soft robots. The authors consider the key challenges the field faces in engineering material, software, and hardware that can bring highly intelligent soft robots into real world.
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http://dx.doi.org/10.1002/advs.202104347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922102PMC
March 2022

Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.

Front Cell Dev Biol 2021 5;9:807862. Epub 2022 Jan 5.

Department of Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

Ovarian cancer remains the most lethal gynecological malignancy. Ferroptosis, a specialized form of iron-dependent, nonapoptotic cell death, plays a crucial role in various cancers. However, the contribution of ferroptosis to ovarian cancer is poorly understood. Here, we characterized the diagnostic, prognostic, and therapeutic value of ferroptosis-related genes in ovarian cancer by analyzing transcriptomic data from The Cancer Genome Atlas and Gene Expression Omnibus databases. A reliable 10-gene ferroptosis signature (HIC1, ACSF2, MUC1, etc.) for the diagnosis of ovarian cancer was identified. Notably, we constructed and validated a novel prognostic signature including three FRGs: HIC1, LPCAT3, and DUOX1. We also further developed a risk score model based on these three genes which divided ovarian cancer patients into two risk groups. Functional analysis revealed that immune response and immune-related pathways were enriched in the high-risk group. Meanwhile, the tumor microenvironment was distinct between the two groups, with more M2 Macrophage infiltration and higher expression of key immune checkpoint molecules in the high-risk group than in the other group. Low-risk patients exhibited more favorable immunotherapy and chemotherapy responses. We conclude that crosstalk between ferroptosis and immunity may contribute to the worse prognosis of patients in the high-risk group. In particular, HIC1 showed both diagnostic and prognostic value in ovarian cancer. experiments demonstrated that inhibition of HIC1 improved drug sensitivity of chemotherapy and immunotherapy agents by inducing ferroptosis. Our findings provide new insights into the potential role of FRGs in the early detection, prognostic prediction, and individualized treatment decision-making for ovarian cancer patients.
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http://dx.doi.org/10.3389/fcell.2021.807862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766510PMC
January 2022

Seasonal variations in the composition and functional profiles of gut microbiota reflect dietary changes in plateau pikas.

Integr Zool 2022 May 17;17(3):379-395. Epub 2022 Feb 17.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Seasonal variations in gut microbiota of small mammals and how they are influenced by environmental variables are relatively poorly understood. We sampled 162 wild plateau pikas (Ochotona curzoniae) in 4 seasons over 2 and a half years and recorded the air temperature, precipitation, and nutrient content in edible vegetation at the sampling site. After conducting 16S rRNA and shotgun metagenomic sequencing, we found that the highest alpha diversity, the relative abundance of Firmicutes, and the simplest co-occurrence network occurred in winter, whereas the highest relative abundance of Proteobacteria and the most complex network structure were observed in spring. The highest relative abundance of Verrucomicrobiota and Spirochaetota was seen in summer and autumn, respectively. Air temperature, precipitation, and the contents of crude protein, crude fiber, and polysaccharide in vegetation had significant effects on the seasonal changes in gut microbiota. Diet contributed more to microbial variation than climatic factors. Metagenomic analysis revealed that the amino acid metabolism pathway and axillary activity enzymes were most abundant in summer, while abundance of carbohydrate-binding modules and carbohydrate esterases was highest in spring. These microbial variations were related to the changes in dietary nutrition, indicating that gut microbiota of plateau pika contribute to the efficient use of food resources. This study provides new evidence of how external environmental factors affect the intestinal environment of small mammals.
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http://dx.doi.org/10.1111/1749-4877.12630DOI Listing
May 2022

Label-Free Diagnosis of Pulmonary Fat Embolism Using Fourier Transform Infrared (FT-IR) Spectroscopic Imaging.

Appl Spectrosc 2022 Mar 12;76(3):352-360. Epub 2022 Jan 12.

Department of Forensic Medicine, 74628Guizhou Medical University, Guizhou, China.

The diagnosis of pulmonary fat embolism (PFE) is of great significance in the field of forensic medicine because it can be considered a major cause of death or a vital reaction. Conventional histological analysis of lung tissue specimens is a widely used method for PFE diagnosis. However, variable and labor-intensive tissue staining procedures impede the validity and informativeness of histological image analysis. To obtain complete information from tissues, a method based on infrared imaging of unlabeled tissue sections was developed to identify pulmonary fat emboli in the present study. We selected 15 PFE-positive lung samples and 15 PFE-negative samples from real cases. Oil red O (ORO) staining and infrared spectral imaging collection were both performed on all lung tissue samples. And the fatty tissue of the abdominal wall and the embolized lipid droplets in the lungs were taken for comparison. The results of the blind, evaluation by pathologists, showed good agreement between the infrared spectral imaging of the lung tissue and the standard histological stained images. Fourier transform infrared (FT-IR) spectroscopic imaging significantly simplifies the typical painstakingly laborious histological staining procedure. And we found a difference between lipid droplets embolized in abdominal wall fat and lung tissue.
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http://dx.doi.org/10.1177/00037028211061430DOI Listing
March 2022

IFP35 as a promising biomarker and therapeutic target for the syndromes induced by SARS-CoV-2 or influenza virus.

Cell Rep 2021 12 3;37(12):110126. Epub 2021 Dec 3.

School of Pharmaceutical Science (Shenzhen), Sun Yat-Sen University, Shenzhen, Guangdong 518107, China. Electronic address:

Previous studies have shown that the high mortality caused by viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza virus primarily results from complications of a cytokine storm. Therefore, it is critical to identify the key factors participating in the cytokine storm. Here we demonstrate that interferon-induced protein 35 (IFP35) plays an important role in the cytokine storm induced by SARS-CoV-2 and influenza virus infection. We find that the levels of serum IFP35 in individuals with SARS-CoV-2 correlates with severity of the syndrome. Using mouse model and cell assays, we show that IFP35 is released by lung epithelial cells and macrophages after SARS-CoV-2 or influenza virus infection. In addition, we show that administration of neutralizing antibodies against IFP35 considerably reduces lung injury and, thus, the mortality rate of mice exposed to viral infection. Our findings suggest that IFP35 serves as a biomarker and as a therapeutic target in virus-induced syndromes.
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http://dx.doi.org/10.1016/j.celrep.2021.110126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639452PMC
December 2021

Single-cell transcriptomic identified HIF1A as a target for attenuating acute rejection after heart transplantation.

Basic Res Cardiol 2021 12 6;116(1):64. Epub 2021 Dec 6.

Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, China.

Acute rejection (AR) is an important contributor to graft failure, which remains a leading cause of death after heart transplantation (HTX). The regulation of immune metabolism has become a new hotspot in the development of immunosuppressive drugs. In this study, Increased glucose metabolism of cardiac macrophages was found in patients with AR. To find new therapeutic targets of immune metabolism regulation for AR, CD45 immune cells extracted from murine isografts, allografts, and untransplanted donor hearts were explored by single-cell RNA sequencing. Total 20 immune cell subtypes were identified among 46,040 cells. The function of immune cells in AR were illustrated simultaneously. Cardiac resident macrophages were substantially replaced by monocytes and proinflammatory macrophages during AR. Monocytes/macrophages in AR allograft were more active in antigen presentation and inflammatory recruitment ability, and glycolysis. Based on transcription factor regulation analysis, we found that the increase of glycolysis in monocytes/macrophages was mainly regulated by HIF1A. Inhibition of HIF1A could alleviate inflammatory cells infiltration in AR. To find out the effect of HIF1A on AR, CD45 immune cells extracted from allografts after HIF1A inhibitor treatment were explored by single-cell RNA sequencing. HIF1A inhibitor could reduce the antigen presenting ability and pro-inflammatory ability of macrophages, and reduce the infiltration of Cd4+ and Cd8a+ T cells in AR. The expression of Hif1α in AR monocytes/macrophages was regulated by pyruvate kinase 2. Higher expression of HIF1A in macrophages was also detected in human hearts with AR. These indicated HIF1A may serve as a potential target for attenuating AR.
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http://dx.doi.org/10.1007/s00395-021-00904-5DOI Listing
December 2021

Insights into the mechanism and regioselectivity in Ni-catalysed redox-relay migratory hydroarylation of alkenes with arylborons.

Chem Commun (Camb) 2021 Dec 14;57(99):13610-13613. Epub 2021 Dec 14.

Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.

DFT studies reveal that Ni-catalysed redox-relay hydroarylation of alkenes occurs concerted hydronickelation, iterative β-H elimination/migratory insertion and reductive elimination to yield the α-substituted product. The driving force for the redox-relay migratory hydroarylation arises from the stability of the LArNi(II)CHPhPr intermediate, which only allows its C-C elimination pathway to be opened up.
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http://dx.doi.org/10.1039/d1cc05125cDOI Listing
December 2021

Prediction of functional microexons by transfer learning.

BMC Genomics 2021 Nov 26;22(1):855. Epub 2021 Nov 26.

College of Intelligent Systems Science and Engineering, Harbin Engineering University, Harbin, China.

Background: Microexons are a particular kind of exon of less than 30 nucleotides in length. More than 60% of annotated human microexons were found to have high levels of sequence conservation, suggesting their potential functions. There is thus a need to develop a method for predicting functional microexons.

Results: Given the lack of a publicly available functional label for microexons, we employed a transfer learning skill called Transfer Component Analysis (TCA) to transfer the knowledge obtained from feature mapping for the prediction of functional microexons. To provide reference knowledge, microindels were chosen because of their similarities to microexons. Then, Support Vector Machine (SVM) was used to train a classification model in the newly built feature space for the functional microindels. With the trained model, functional microexons were predicted. We also built a tool based on this model to predict other functional microexons. We then used this tool to predict a total of 19 functional microexons reported in the literature. This approach successfully predicted 16 out of 19 samples, giving accuracy greater than 80%.

Conclusions: In this study, we proposed a method for predicting functional microexons and applied it, with the predictive results being largely consistent with records in the literature.
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http://dx.doi.org/10.1186/s12864-021-08187-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627023PMC
November 2021

Low-/high-density lipoprotein cholesterol ratio and carotid plaques in patients with coronary heart disease: a Chinese cohort study.

Lipids Health Dis 2021 Oct 27;20(1):144. Epub 2021 Oct 27.

Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.

Background: Evidence on the relationship between the low-/high-density lipoprotein cholesterol ratio (LDL-C/HDL-C) and carotid plaques remains limited. This study aimed to examine the association between LDL-C/HDL-C and carotid plaques in participants with coronary heart disease (CHD) and to further explore the extent to which a healthy lifestyle reduces the risk of LDL-C/HDL-C-related carotid plaques.

Methods: This large-scale and multi-centre retrospective study included 9426 CHD patients (aged 35-75 years) between January 1, 2014 and September 30, 2020. The LDL-C/HDL-C values were converted to the following tertiles: lowest (< 2.15), middle (2.15-3), and highest (> 3). Healthy lifestyle-related factors referred to whether or not the participant was a non-smoker and non-drinker. Participants were divided into an unfavourable group (those who did not adhere to healthy lifestyle factors), intermediate (only one unhealthy factor), and favourable (neither of the two unhealthy factors). Logistic regression was used for statistical analyses.

Results: Of the 9426 participants, 6989 (74.15%) CHD patients had carotid plaques. After adjustment for confounders, each unit increase in the LDL-C/HDL-C was significantly associated with carotid plaques (OR: 1.61; 95%CI: 1.43-1.84; P <  0.001). Multivariate logistic regression revealed that carotid plaques risk for the highest tertile (> 3) was 1.18 times that of the lowest quartile (< 2.15). Compared with an unfavourable lifestyle, an intermediate or a favourable lifestyle was associated with a significant 30% (OR: 0.70; 95%CI: 0.64-0.78; P <  0.001) or 67% (OR: 0.33; 95%CI: 0.29-0.37; P <  0.001) reduction in carotid plaques risk, respectively, among CHD patients with high LDL-C/HDL-C. There were significantly additive and multiplicative interactions between lifestyle and LDL-C/HDL-C with regards to carotid plaques.

Conclusion: A high LDL-C/HDL-C is associated with a risk of carotid plaques developing in CHD patients. Adhering to a healthy lifestyle has additive beneficial effects on reducing the risk of carotid plaques, especially in relation to the highest LDL-C/HDL-C.
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http://dx.doi.org/10.1186/s12944-021-01575-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549164PMC
October 2021
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