Publications by authors named "Qi Chen"

2,762 Publications

  • Page 1 of 1

Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times.

Plant Pathol J 2022 Aug 1;38(4):287-295. Epub 2022 Aug 1.

Citrus Research Institute, Southwest University, Chongqing 400712, China.

Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants.
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http://dx.doi.org/10.5423/PPJ.OA.01.2022.0011DOI Listing
August 2022

SIAH1-mediated RPS3 ubiquitination contributes to chemosensitivity in epithelial ovarian cancer.

Aging (Albany NY) 2022 Aug 8;14(undefined). Epub 2022 Aug 8.

Reproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.

The E3 ligase SIAH1 is deregulated in human cancers and correlated with poor prognosis, but its contributions to chemoresistance in epithelial ovarian cancer (EOC) are not evident. Herein we found that SIAH1 was decreased in EOC tumour tissues and cell lines and negatively correlated with the RPS3 levels. SIAH1 overexpression suppressed tumour cell growth, colony formation, invasion, metastasis, and cisplatin resistance and . SIAH1 promoted RPS3 ubiquitination and degradation using the RING-finger domain, and these steps were required for RPS3 localization to the cytoplasm, which led to subsequent NF-κB inactivation and thereby conferred chemosensitivity. Moreover, ectopic expression of RPS3 or depletion of RPS3 ubiquitination mediated by SIAH1 via the K214R mutant significantly impaired cisplatin-induced tumour suppression in cells stably expressing SIAH1. Together, our findings reveal a tumour suppressor function of SIAH1 and provide evidence showing that the SIAH1-RPS3-NF-κB axis may act as an appealing strategy for tackling treatment resistance in EOC.
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http://dx.doi.org/10.18632/aging.204211DOI Listing
August 2022

Reversible phase transition for durable formamidinium-dominated perovskite photovoltaics.

Adv Mater 2022 Aug 10:e2204458. Epub 2022 Aug 10.

Beijing Key Laboratory for Theory and Technology of Advanced Battery Materials, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, School of Materials Science and Engineering, Peking University, Beijing, 100871, P. R. China.

Phase instability (especially phase transition) is one of the major obstacles to the wide application of formamidinium (FA)-dominated perovskite solar cells (PSCs). An in-depth investigation on relevant phase transition kinetics is urgently needed to explore more effective phase stabilization strategies. Herein for the first time, we monitored the reversible phase transition process of FA Cs PbI perovskite between photoactive phase (α phase) and non-photoactive phase (δ phase) under humidity, as well as the reversible healing of the degraded devices. Moreover, through in-situ atomic force microscope (AFM) characterization, the kinetic transition between α and δ phase was revealed to be the "nucleation-growth transition" process. Density functional theory (DFT) calculation was employed to analyze the thermodynamic origin of phase transition, where the driving force for α-to-δ transition was associated with configurational enthalpy, while the δ-to-α transition was dominated by the temperature-related vibrational entropy. The α phase of FA Cs PbI could be finally stabilized at elevated temperature (e.g., 60°C) under high humidity due to the increased nucleation barrier, and the resulting non-encapsulated PSCs retained over 90% of their initial efficiency after aging at 60°C and 60% relative humidity (RH) for 1000 hours. Our finding provided a deepened understanding on the phase transition process of FA Cs PbI from both thermodynamics and kinetics points of view, which also presented an effective means to stabilize the α phase of FA-dominated perovskites and devices for practical applications. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202204458DOI Listing
August 2022

Peripapillary structural and microvascular alterations in early dysthyroid optic neuropathy.

Eye Vis (Lond) 2022 Aug 9;9(1):30. Epub 2022 Aug 9.

School of Ophthalmology and Optometry, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou, 325027, Zhejiang, China.

Background: To explore the changes in blood supply and structure around the optic nerve head (ONH) in thyroid-associated ophthalmopathy (TAO) patients with suspected dysthyroid optic neuropathy (DON).

Methods: TAO patients [19 with DON; 24 non-DON (NDON); 20 with equivocal DON (EDON)], and 34 control subjects were examined. Optical coherence tomography angiography (OCTA) was used to obtain peripapillary retinal nerve fiber layer (p-RNFL) and vessel density parameters, including the ONH whole image vessel density (ONH-wiVD) and the radial peripapillary capillary vessel density (RPC-VD) in early DON.

Results: Although there were no differences in p-RNFL thickness among the groups, there were differences in the ONH-wiVD of each grid section and the RPC-VD in all areas (P < 0.01). Compared with healthy controls, the EDON eyes had significantly lower RPC-VDs in all aeras (P < 0.05).The peripapillary region was further divided into eight sectors, and the RPC-VD in the temporal upper, superior temporal, and temporal lower sectors in the EDON group were significantly lower than in the controls. The visual impairment was closely related to the loss of peripapillary capillary vessel density. Univariate correlation analysis showed that the ONH-wiVD and RPC-VD of the TAO groups were negatively correlated with the intraocular pressure (r = - 0.296, P = 0.006; r = - 0.258, P = 0.016 respectively).

Conclusions: EDON patients had significantly lower ONH-wiVD and RPC-VD than control subjects, and the temporal and upper VDs were more likely to be affected in the early stage of TAO. The combined use of spectral domain optical coherence tomography and OCTA technologies offer a new method for early diagnosis of suspected DON patients.
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http://dx.doi.org/10.1186/s40662-022-00301-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361673PMC
August 2022

Dynamic Predictive Models With Visualized Machine Learning for Assessing Chondrosarcoma Overall Survival.

Front Oncol 2022 21;12:880305. Epub 2022 Jul 21.

Faculty of Medicine, Macau University of Science and Technology, Macao SAR, China.

Chondrosarcoma is a malignant bone tumor with a low incidence rate. Accurate risk evaluation is crucial for chondrosarcoma treatment. Due to the limited reliability of existing predictive models, we intended to develop a credible predictor for clinical chondrosarcoma based on the Surveillance, Epidemiology, and End Results data and four Chinese medical institutes. Three algorithms (Best Subset Regression, Univariate and Cox regression, and Least Absolute Shrinkage and Selector Operator) were used for the joint training. A nomogram predictor including eight variables-age, sex, grade, T, N, M, surgery, and chemotherapy-is constructed. The predictor provides good performance in discrimination and calibration, with area under the curve ≥0.8 in the receiver operating characteristic curves of both internal and external validations. The predictor especially had very good clinical utility in terms of net benefit to patients at the 3- and 5-year points in both North America and China. A convenient web calculator based on the prediction model is available at https://drwenle029.shinyapps.io/CHSSapp, which is free and open to all clinicians.
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http://dx.doi.org/10.3389/fonc.2022.880305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351692PMC
July 2022

Peritumor Edema Serves as an Independent Predictive Factor of Recurrence Patterns and Recurrence-Free Survival for High-Grade Glioma.

Comput Math Methods Med 2022 27;2022:9547166. Epub 2022 Jul 27.

School of Medical Imaging, Xuzhou Medical University, Xuzhou 221000, China.

Objective: This study is aimed at analyzing the factors affecting the recurrence patterns and recurrence-free survival (RFS) of high-grade gliomas (HGG).

Methods: Eligible patients admitted to the Affiliated Hospital of Xuzhou Medical University were selected. Subsequently, the effects of some clinical data including age, gender, WHO pathological grades, tumor site, tumor size, clinical treatments, and peritumoral edema (PTE) area and molecular markers (Ki-67, MGMT, IDH-1, and p53) on HGG patients' recurrence patterns and RFS were analyzed.

Results: A total number of 77 patients were enrolled into this study. After analyzing all the cases, it was determined that tumor size and tumor site had a significant influence on the recurrent patterns of HGG, and PTE was an independent predict factor of recurrence patterns. Specifically, when the PTE was mild (<1 cm), the recurrence pattern tended to be local; in contrast, HGG was more likely to progress to marginal recurrence and distant recurrence. Furthermore, age and PTE were significantly associated with RFS; the median RFS of the population with PTE < 1 cm (23.60 months) was obviously longer than the population with PTE ≥ 1 cm (5.00 months).

Conclusions: PTE is an independent predictor of recurrence patterns and RFS for HGG. Therefore, preoperative identification of PTE in HGG patients is crucially important, which is helpful to accurately estimate the recurrence pattern and RFS.
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http://dx.doi.org/10.1155/2022/9547166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348930PMC
August 2022

Association between short-term exposure to ambient air pollution and dementia mortality in Chinese adults.

Sci Total Environ 2022 Aug 4:157860. Epub 2022 Aug 4.

Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address:

Background: Short-term exposure to ambient air pollution has been linked to an increased risk of mortality from a variety of causes, but its effects on mortality from dementia remain largely unknown.

Objectives: To investigate the association between short-term exposure to ambient air pollution and dementia mortality, and quantitatively assess the excess mortality.

Methods: In this time-stratified case-crossover study, 47,108 dementia deaths were identified in Jiangsu province, China during 2015-2019. Exposure to particulate matter with an aerodynamic diameter ≤ 2.5 μm (PM), PM, sulfur dioxide (SO), nitrogen dioxide (NO), carbon monoxide (CO), and ozone (O) was assessed by extracting daily concentrations from a validated grid dataset based on each subject's residential address. Conditional logistic regression models were applied for exposure-response analyses.

Results: There were 47,108 case days and 159,852 control days during the study period. Each 10 μg/m increase of lag 04-day exposure to PM, PM, and NO was significantly associated with a 1.43 % (95 % CI: 0.77, 2.09 %), 1.06 % (0.59, 1.54 %), and 2.80 % (1.51, 4.10 %) increase in odds of dementia mortality, corresponding to an excess mortality of 4.87 %, 5.50 %, and 6.43 %, respectively. We estimated that reducing ambient air pollutant exposures to the WHO air quality guidelines would avoid up to 4.17 % of the dementia deaths, while the ambient air quality standards in China would only help avoid up to 0.39 %.

Conclusions: This study provides consistent evidence that short-term exposure to PM, PM, and NO is associated with increased risk of dementia mortality, which can be translated to a considerable excess mortality. Our findings highlight a potential approach to prevent deaths from dementia by reducing individual exposures to ambient air pollution, especially in areas with high levels of ambient air pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157860DOI Listing
August 2022

Clinical, Pathological Complete Response, and Prognosis Characteristics of HER2-Low Breast Cancer in the Neoadjuvant Chemotherapy Setting: A Retrospective Analysis.

Ann Surg Oncol 2022 Aug 6. Epub 2022 Aug 6.

Department of Breast Oncology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, China.

Background: The present study was conducted to evaluate the clinical, pathological response, and prognosis characteristics of human epidermal growth factor receptor 2 (HER2)-low breast cancer in the neoadjuvant chemotherapy setting.

Methods: Patients with HER2-negative breast cancer who received neoadjuvant chemotherapy from January 2017 to December 2019 were retrospectively analyzed. HER2-negative breast cancer was divided into two groups: HER2-zero (defined as immunohistochemistry [IHC] 0) and HER2-low (defined as IHC 1+, or IHC 2+ and fluorescence in-situ hybridization-negative.

Results: Overall, 314 patients with HER2-negative breast cancer were analyzed. The proportion of HER2-low patients with hormone receptor (HR)-positive disease was higher than in triple-negative breast cancer (TNBC; 75.3% vs. 63.2%, p = 0.032). In HR-positive breast cancer, HER2-low tumors presented less nodal involvement (p = 0.023) and earlier clinical stage (p = 0.015) compared with HER2-zero tumors; however, in TNBC, HER2-low patients had a later clinical stage (p = 0.028). With the pathological complete response (pCR) defined as ypTis/0ypN0, there was no difference in pCR rates among the entire cohort, HR-positive disease, and TNBC. However, with the pCR defined as ypT0ypN0, the pCR rate in HER2-low breast cancer was significantly lower than HER2-zero breast cancer in the entire cohort (24.3% vs. 36.4%, p = 0.032) and the HR-positive subgroup (18.7% vs. 32.1%, p = 0.035), but not for TNBC. Multivariate analysis demonstrated that HER2 status (low vs. zero) was an independent predictive factor for pCR (p = 0.013) in HR-positive breast cancer. There were no statistically significant differences in 3-year disease-free survival and overall survival between HER2-low and HER2-zero breast cancer among the entire cohort, HR-positive disease, and TNBC.

Conclusions: HER2-low breast cancer exhibits specific clinical features and different response to treatment associated with HR status in the neoadjuvant chemotherapy setting.
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http://dx.doi.org/10.1245/s10434-022-12369-4DOI Listing
August 2022

Deep Learning for Automated Contouring of Gross Tumor Volumes in Esophageal Cancer.

Front Oncol 2022 18;12:892171. Epub 2022 Jul 18.

Department of Radiation Oncology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China.

Purpose: The aim of this study was to propose and evaluate a novel three-dimensional (3D) V-Net and two-dimensional (2D) U-Net mixed (VUMix-Net) architecture for a fully automatic and accurate gross tumor volume (GTV) in esophageal cancer (EC)-delineated contours.

Methods: We collected the computed tomography (CT) scans of 215 EC patients. 3D V-Net, 2D U-Net, and VUMix-Net were developed and further applied simultaneously to delineate GTVs. The Dice similarity coefficient (DSC) and 95th-percentile Hausdorff distance (95HD) were used as quantitative metrics to evaluate the performance of the three models in ECs from different segments. The CT data of 20 patients were randomly selected as the ground truth (GT) masks, and the corresponding delineation results were generated by artificial intelligence (AI). Score differences between the two groups (GT versus AI) and the evaluation consistency were compared.

Results: In all patients, there was a significant difference in the 2D DSCs from U-Net, V-Net, and VUMix-Net (p=0.01). In addition, VUMix-Net showed achieved better 3D-DSC and 95HD values. There was a significant difference among the 3D-DSC (mean ± STD) and 95HD values for upper-, middle-, and lower-segment EC (p<0.001), and the middle EC values were the best. In middle-segment EC, VUMix-Net achieved the highest 2D-DSC values (p<0.001) and lowest 95HD values (p=0.044).

Conclusion: The new model (VUMix-Net) showed certain advantages in delineating the GTVs of EC. Additionally, it can generate the GTVs of EC that meet clinical requirements and have the same quality as human-generated contours. The system demonstrated the best performance for the ECs of the middle segment.
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http://dx.doi.org/10.3389/fonc.2022.892171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339638PMC
July 2022

Rare delayed cardiac tamponade in a pig after cardiac surgery.

Vet Med Sci 2022 Aug 3. Epub 2022 Aug 3.

Center for Preclinical Surgical and Interventional Research, Texas Heart Institute, Houston, Texas, USA.

Objective: Delayed cardiac tamponade, a life-threatening complication of pericardial effusion in humans, has rarely been described in large animal models. We report here a pig with cardiac tamponade that developed 29 days after cardiac surgery.

Study Design: Case report.

Animals: One 45-kg domestic pig.

Methods: Open-chest surgery was performed on a pig to induce chronic heart failure. At 15 days after surgery, the pig's breathing appeared laboured; induced heart failure was considered the cause. Routine heart failure medications were administered.

Results: On day 28, the pig's status deteriorated. On day 29, echocardiography performed just before the pig's death showed a large pericardial effusion, mainly in the lateral and anterior walls of the right heart, with several fibre exudation bands. The right heart was severely compressed with an extremely small right ventricle. An emergency sternotomy was unsuccessful. Pathologic examination showed a severely thickened, fibrous pericardium. The pericardial sac was distended (up to 4.5 cm) and was full of dark brown, soft, friable material. Epicardial haemorrhage with a fresh, organised thrombus was noted in the pericardium.

Conclusion: Delayed tamponade occurring at least 15 days after open-chest surgery is easy to misdiagnose or overlook in large animal models where attention is often focused on primary pathological model changes. To decrease mortality in animal models, researchers should be aware of potential complications and use the same level of follow-up monitoring of large animals as in clinical care.
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http://dx.doi.org/10.1002/vms3.892DOI Listing
August 2022

Analgesic effect of ultrasound-guided erector spinae plane block (espb) in general anesthesia for cesarean section: a randomized controlled trial.

BMC Anesthesiol 2022 08 2;22(1):244. Epub 2022 Aug 2.

Department of Anesthesiology, Woman and Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: The analgesic effects of erector spinae plane block in general anesthesia for cesarean section and recovery from puerperae remain unclear.

Methods: Sixty patients with contraindications for spinal anesthesia who required general anesthesia for cesarean section were enrolled and randomly divided into the erector spinal plane block (ESPB) combined with the general anesthesia group (group E) and general anesthesia group (group G). Group E received bilateral ESPB (20 ml of 0.25% ropivacaine on each side) under ultrasound guidance 30 min before general anesthesia. The primary outcomes were the number of patient-controlled intravenous analgesia (PCIA) boluses, and Bruggemann comfort scale (BCS) scores at 2 h, 6 h, 12 h, and 24 h after operation. The second outcome was intraoperative anesthesia dosage, fetal delivery time, puerperae emergence time, visual analog scale (VAS) at 2 h, 6 h, 12 h, and 24 h after operation, and incidence of nausea and vomiting. Heart rate (HR) and mean arterial pressure (MAP) were recorded 10 min before the start of anesthesia (T0), at the induction of anesthesia (T1), at skin incision (T2), and fetal delivery (T3), and immediately after surgery (T4).

Results: The number of PCIA boluses was lower in group E than in group G (P < 0.001). The BCS score increased at 2 h and 6 h after the operation in group E (P < 0.05), while the VAS score significantly decreased in group E at the same time (P < 0.05). Compared with group G, the doses of propofol and remifentanil were significantly decreased in group E (P < 0.001), the emergence time of puerperae was shortened (P = 0.003), and the incidence of nausea and vomiting was significantly decreased (P = 0.014).

Conclusion: Ultrasound-guided ESPB applied to general anesthesia for a cesarean section can significantly reduce the required dose of general anesthetic drugs, shorten the recovery time of the puerperae, and improve postoperative analgesia.

Trial Registration: www.

Clinicaltrials: gov under the number ChiCTR2200056337 (04-02-2022).
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http://dx.doi.org/10.1186/s12871-022-01781-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344714PMC
August 2022

Particulate matter and ozone might trigger deaths from chronic ischemic heart disease.

Ecotoxicol Environ Saf 2022 Jul 30;242:113931. Epub 2022 Jul 30.

Department of Environment and Health, Jiangsu Provincial Center for Disease Control and Prevention, 172 Jiangsu Road, Nanjing, Jiangsu, 210009, China. Electronic address:

Aims: To study the association between short-term exposure to air pollutants and mortality of Chronic Ischemic Heart Disease (CIHD).

Methods: Using a case-crossover design, we investigated 148,443 CIHD deaths from 2015 to 2020 in Jiangsu Province, China. Exposure to six ambient pollutants, including PM, PM, NO, CO, SO, and O was assessed by extracting daily concentrations from validated 10 km × 10 km pollutant grids at each subject's residential address. A conditional logistic regression approach was used to explore the exposure-response relationship with adjustment for temperature and relative humidity. We calculated the Population Attributable Fractions (PAFs) and the attributable deaths number of CIHD.

Results: An increase of 10 μg/m in PM and PM exposure was associated with a 1.16% (95% CI: 0.85-1.48%) and 1.80% (1.36-2.24%) increase in CIHD mortality, respectively. A threshold value of 123 µg/m was identified for the association between O exposure and CIHD mortality. Controlling for PM, each increase of 10 µg/m in O (>threshold) was statistically significantly associated with a 0.94% (0.19-1.71%) increase in CIHD mortality, however there was no association between NO, SO, CO exposure and CIHD mortality. Reducing PM, PM and O to the WHO air quality guidelines would prevent 6.16% (95% CI: 4.70-7.58%), 4.30% (3.18-5.43%) and 1.29% (0.48-4.20%) of CIHD deaths, respectively. During the warm season, mortality and PAFs of CIHD associated with PM, PM, and O were significantly higher.

Conclusions: Short-term exposure to ambient PM, PM and O might trigger deaths from CIHD. These findings indicate that the premature deaths of CIHD patients can be alleviated by reducing exposure to polluted air.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113931DOI Listing
July 2022

Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases.

Chem Commun (Camb) 2022 Aug 1. Epub 2022 Aug 1.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Fungal bifunctional terpene synthases (BFTSs) reportedly associate with a series of new skeletons of di/sesterterpenes. However, the molecular mechanisms underlying the variabilities in the ring system of BFTS-catalyzed products are not well understood. In this study, we identified a key site, S89/L89, that controls the conversion between bicyclic and polycyclic terpene skeletons catalyzed by two BFTSs, BsPS and FoFS. Our analysis revealed that a mutation on site 89 in the BFTSs alters the carbocation transportation pathway and redirects the competing reactions for previously unreported terpenes.
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http://dx.doi.org/10.1039/d2cc03644dDOI Listing
August 2022

Genetic Diversity, Evolutionary Dynamics, and Pathogenicity of Ferret Badger Rabies Virus Variants in Mainland China, 2008-2018.

Front Microbiol 2022 14;13:929202. Epub 2022 Jul 14.

Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China.

In contrast to dog-associated human rabies cases decline year by year due to the rabies vaccination coverage rates increase in China, ferret badger (FB, )-associated human rabies cases emerged in the 1990s, and are now an increasingly recognized problem in southeast China. To investigate epidemiology, temporal evolution dynamics, transmission characterization, and pathogenicity of FB-associated rabies viruses (RABVs), from 2008 to 2018, we collected 3,622 FB brain samples in Jiangxi and Zhejiang Province, and detected 112 RABV isolates. Four FB-related lineages were identified by phylogenetic analysis (lineages A-D), the estimated Times to Most Recent Common Ancestor were 1941, 1990, 1937, and 1997 for lineages A-D, respectively. Furthermore, although no FB-associated human rabies case has been reported there apart from Wuyuan area, FB-RABV isolates are mainly distributed in Jiangxi Province. Pathogenicity of FB-RABVs was assessed using peripheral inoculation in mice and in beagles with masseter muscles, mortality-rates ranging from 20 to 100% in mice and 0 to 20% in beagles in the groups infected with the various isolates. Screening of sera from humans with FB bites and no post-exposure prophylaxis to rabies revealed that five of nine were positive for neutralizing antibodies of RABV. All the results above indicated that FB-RABV variants caused a lesser pathogenicity in mice, beagles, and even humans. Vaccination in mice suggests that inactivated vaccine or recombinant subunit vaccine products can be used to control FB-associated rabies, however, oral vaccines for stray dogs and wildlife need to be developed and licensed in China urgently.
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http://dx.doi.org/10.3389/fmicb.2022.929202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330412PMC
July 2022

Correction: hUC-EVs-ATO reduce the severity of acute GVHD by resetting inflammatory macrophages toward the M2 phenotype.

J Hematol Oncol 2022 Jul 31;15(1):102. Epub 2022 Jul 31.

Peking University People's Hospital, Peking University Institute of Hematology, No. 11 Xizhimen South Street, Xicheng District, Beijing, China.

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http://dx.doi.org/10.1186/s13045-022-01323-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339190PMC
July 2022

Comparison of HPV neutralizing and IgG antibodies in unvaccinated female adolescents.

Future Microbiol 2022 Jul 29. Epub 2022 Jul 29.

State Key Laboratory of Molecular Vaccinology & Molecular Diagnostics, National Institute of Diagnostics & Vaccine Development in Infectious Diseases, Strait Collaborative Innovation Center of Biomedicine and Pharmaceutics, School of Public Health, Xiamen University, Xiamen, 361102, Fujian, China.

To analyze the consistency between HPV neutralizing antibodies and specific total IgG antibodies in unvaccinated females. Serum samples from 978 unvaccinated Chinese females aged 9-26 years were measured for antibodies against HPV-16 and HPV-18 using simultaneous pseudovirus-based neutralization assay and ELISA. There was a moderate level of consistency between HPV neutralizing antibodies and specific IgG in females aged 18-26 years (Cohen's κ coefficient for HPV-16 and HPV-18: 0.52 and 0.38) and poor consistency in those aged 9-17 years (Cohen's κ coefficient <0.05). However, Cohen's κ coefficient remained almost unchanged in sensitivity analysis when the IgG antibody cut-off value was raised. HPV neutralizing antibodies are a more specific indicator for the evaluation of HPV natural humoral immunity.
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http://dx.doi.org/10.2217/fmb-2021-0221DOI Listing
July 2022

Fabric-based synthesis of gold nanoparticles for continuous enhanced heterogeneous chemiluminescence online detection of carbon dioxide.

Analyst 2022 Jul 28. Epub 2022 Jul 28.

School of Chemistry and Environmental Engineering, Sichuan University of Science & Engineering, Zigong 643000, China.

In this paper, nylon microfiber fabric with a heterogeneous chemiluminescence system was used for the synthesis of gold nanoparticles (AuNPs) for the first time, and it was used to enhance the online detection of carbon dioxide (CO) by heterogeneous chemiluminescence. Nylon microfiber fabric was chosen as the gas-liquid heterogeneous chemiluminescence micro reaction interface. The thermal reduction method prepared the micro reaction interface with synthesized AuNPs. The solution of HO and KOH forms a liquid film on the surface of the micro reaction interface, and a heterogeneous chemiluminescence reaction occurs when CO flows through the surface of the liquid film. The AuNPs supported on the micro reaction interface act catalytically and enhance the chemiluminescence signal for CO detection. In this way, AuNPs can be used repeatedly in the chemiluminescence reaction, avoiding the waste of precious metal nanoparticles and reducing the detection cost. Under the optimal conditions, CO has a good linear relationship with the chemiluminescence signal in the range of 10-20 000 ppm (v/v). The correlation coefficient is 0.9963, the detection limit is 0.35 ppm (v/v), and the relative standard deviation is 1.79%. This approach provides an entirely new technological platform for the development of functional fabrics and the maximum utilization of precious metal nanoparticles.
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http://dx.doi.org/10.1039/d2an00721eDOI Listing
July 2022

The Emerging Role of Immune Cells and Targeted Therapeutic Strategies in Diabetic Wounds Healing.

J Inflamm Res 2022 20;15:4119-4138. Epub 2022 Jul 20.

Department of Endocrinology and Metabolism, the Affiliated Hospital of Southwest Medical University, Luzhou, People's Republic of China.

Poor wound healing in individuals with diabetes has long plagued clinicians, and immune cells play key roles in the inflammation, proliferation and remodeling that occur in wound healing. When skin integrity is damaged, immune cells migrate to the wound bed through the actions of chemokines and jointly restore tissue homeostasis and barrier function by exerting their respective biological functions. An imbalance of immune cells often leads to ineffective and disordered inflammatory responses. Due to the maladjusted microenvironment, the wound is unable to smoothly transition to the proliferation and remodeling stage, causing it to develop into a chronic refractory wound. However, chronic refractory wounds consistently lead to negative outcomes, such as long treatment cycles, high hospitalization rates, high medical costs, high disability rates, high mortality rates, and many adverse consequences. Therefore, strategies that promote the rational distribution and coordinated development of immune cells during wound healing are very important for the treatment of diabetic wounds (DW). Here, we explored the following aspects by performing a literature review: 1) the current situation of DW and an introduction to the biological functions of immune cells; 2) the role of immune cells in DW; and 3) existing (or undeveloped) therapies targeting immune cells to promote wound healing to provide new ideas for basic research, clinical treatment and nursing of DW.
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http://dx.doi.org/10.2147/JIR.S371939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309318PMC
July 2022

Non-contrast enhanced silent MR angiography to evaluate hemodynamics and morphology of unruptured intracranial aneurysms: a comparative computational fluid dynamics study.

J Neurointerv Surg 2022 Jul 26. Epub 2022 Jul 26.

Department of Neurosurgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China

Background: Silent MR angiography (silent MRA) is a new generation of non-contrast enhanced angiography with outstanding advantages in visualizing cerebrovascular lesions and the follow-up after endovascular treatment for intracranial aneurysms (IAs). This study aims to investigate the reliability of silent MRA-based three-dimensional (3D) geometric description and hemodynamic calculation of IAs.

Methods: 19 patients with 23 unruptured IAs, who underwent both silent MRA and 3D rotational angiography (3DRA), were included in this study. Computational fluid dynamics simulations were performed on all patient-specific 3D reconstruction images to compare the morphology and hemodynamics of the two different imaging models for IAs.

Results: Silent MRA models had smaller maximum and perpendicular height (mm), aneurysmal surface area (mm), and aneurysmal volume (mm) than 3DRA (p<0.05); the differences of the above parameters between the two models were 9.0±6.2%, 7.7±7.4%, 15.9±13.0%, and 21.4±17.5%, respectively. However, correlation analysis of morphological parameters in various dimensions and model comparison showed good overall consistency in geometrical characteristics between the two models. Moderate coherence was observed between models in time-averaged wall shear stress of aneurysm and parent vessel (TAWSS, PAWSS), aneurysm velocity (AV), parent vessel velocity, and oscillatory shear index (OSI). However, strong correlations were observed among normalized aneurysm wall shear stress (NWSS), low shear area (LSA), inflow concentration index (ICI), and normalized aneurysm velocity (NAV).

Conclusion: Both morphological and hemodynamic assessments of IAs for silent MRA are comparable to 3DRA. Additionally, normalized indicators such as NWSS, LSA, ICI, and NAV were better than TAWSS, AV, and OSI in silent MRA-related hemodynamic evaluation.
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http://dx.doi.org/10.1136/jnis-2022-018901DOI Listing
July 2022

Immune regulatory effects of microRNA9-3.

Blood Cells Mol Dis 2022 11 19;97:102697. Epub 2022 Jul 19.

Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fuzhou, Fujian Province 350117, China. Electronic address:

MicroRNAs are known to regulate cell proliferation, differentiation, and apoptosis. However, the immunological mechanism and role of microRNA9-3 (miR9-3) are unknown. This study used CRISPR/cas9 technology to knock out miR9-3 to modulate its expression level. FACS results showed that the absolute number of total B cells declined in miR9-3-deficiency in the spleen (Sp), bone marrow (BM), and lymph node (LN) to different levels compared to the wild-type. Also, the absolute numbers of Fo, T1, and T2 cells decreased both in Sp and LN. The absolute numbers of total T cells in Sp and LN declined sharply; CD4 and CD8 T cells showed a dramatic decrease in Sp, LN, and Th (thymus) of the miR9-3 group. In BM, the cells number of immature B cells, pro-pre-B cells, pro-B cells, and pre-B cells reduced to different levels, while mature B cells were comparable to wild-type. These data illustrated that miR9-3-deficiency impaired the development of B cells in BM. Also, the development of T cells was severely impaired. In Th, the numbers of DN and DP cells were remarkably reduced in the miR9-3 mutant mice. Also, the numbers of DN-1, DN-3, and DN-4 cells decreased. The absolute number of cells in the hematopoietic stem cell (HSC) system such as LT-HSC (long-term HSC), ST-HSC (short-term HSC), MPP (multipotent progenitor), GMP (granulocyte-macrophage progenitor), CMP (common myeloid progenitors), MEP (megakaryocyte-erythroid progenitor), and CLP (common lymphoid progenitor) all were decreased in miR9-3 deficient mice. These results showed that miR9-3 deficiency initiated the damage to the entire hematopoietic system. Moreover, the absolute number of myeloid cells in both Sp and BM decreased in mutant mice. The cells number of NK cells showed a sharp reduction in Sp whereas the change was not significant in BM. The above results suggest that miR9-3 participates in the immune regulation of B cells, T cells, and the HSC system, highlighting its regulatory roles.
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http://dx.doi.org/10.1016/j.bcmd.2022.102697DOI Listing
November 2022

Mechanism of Action of Yin Nourishing and Heat Clearing Prescription in Treating Cough Variant Asthma Based on Network Pharmacology and Molecular Docking Verification.

Comput Math Methods Med 2022 12;2022:7518109. Epub 2022 Jul 12.

Department of Traditional Chinese Medicine, The Sixth Medical Center, General Hospital of Chinese PLA, Beijing 100853, China.

Objective: To explore the mechanism of action of the yin nourishing and heat clearing prescription in treating cough variant asthma (CVA) based on network pharmacology (NP).

Methods: The active ingredients and targets of the yin nourishing and heat clearing prescription were screened using the Traditional Chinese Medicine System Pharmacology Analysis Platform (TCMSP); CVA targets were screened by the GeneCards, NCBI gene, and OMIM databases to construct the component-target network and the protein-protein interaction (PPI) network. GO functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the target genes were performed to construct the component-disease-pathway-target biological network. Moreover, CVA-related core target structures with high values were subjected to molecular docking (MD) with the active components.

Results: We found 265 eligible targets in the prescription and 1115 CVA-related genes. The medicine targets were intersected with disease targets, which yielded 148 common targets. After topology analysis, 66 key targets were screened. Upon GO functional annotation, 2408 biological processes, 153 molecular functions, and 162 KEGG pathways were enriched. Molecular docking results suggested that the major active ingredients of the prescription showed high affinity to the key targets, among which AKT1 might be the most important target.

Conclusions: Active ingredients might act on AKT1, IL-6, VEGFA, IL-1B, and JUN to suppress eosinophil accumulation, decrease histamine release, suppress airway inflammation, regulate the airway immune microenvironment, increase autophagy in lung tissue, inhibit mucus production, and reduce airway resistance and hyperresponsiveness, thus treating CVA. Our findings provide a reference for further research and clinical applications of the prescription.
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http://dx.doi.org/10.1155/2022/7518109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296347PMC
July 2022

hUC-EVs-ATO reduce the severity of acute GVHD by resetting inflammatory macrophages toward the M2 phenotype.

J Hematol Oncol 2022 07 21;15(1):99. Epub 2022 Jul 21.

Peking University People's Hospital, Peking University Institute of Hematology, No. 11 Xizhimen South Street, Xicheng District, Beijing, China.

Background: Both extracellular vesicles from mesenchymal stromal cell-derived human umbilical cords (hUC-EVs) and arsenic trioxides (ATOs) have been demonstrated to treat acute graft-versus-host disease (aGVHD) via immunomodulation. Apart from immunomodulation, hUC-EVs have a unique function of drug delivery, which has been proposed to enhance their efficacy. In this study, we first prepared ATO-loaded hUC-EVs (hUC-EVs-ATO) to investigate the therapeutic effect and potential mechanisms of hUC-EVs-ATO in a mouse model of aGVHD after allogeneic hematopoietic stem cell transplantation (HSCT).

Methods: An aGVHD model was established to observe the therapeutic effects of hUC-EVs-ATO on aGVHD. Target organs were harvested for histopathological analysis on day 14 after transplantation. The effects of hUC-EVs-ATO on alloreactive CD4 were evaluated by flow cytometry in vivo and in vitro. Flow cytometry, RT-PCR, immunofluorescence colocalization analysis and Western blot (Wb) analysis were performed to examine macrophage polarization after hUC-EV-ATO treatment. The cytokines in serum were measured by a cytometric bead array (CBA). TEM, confocal microscopy and Wb were performed to observe the level of autophagy in macrophages. A graft-versus-lymphoma (GVL) mouse model was established to observe the role of hUC-EVs-ATO in the GVL effect.

Results: The clinical manifestations and histological scores of aGVHD in the hUC-EVs-ATO group were significantly reduced compared with those in the ATO and hUC-EVs groups. The mice receiving hUC-EVs-ATO lived longer than the control mice. Notably, hUC-EVs-ATO interfering with alloreactive CD4 T cells differentiation were observed in aGVHD mice but not in an in vitro culture system. Additional studies showed that depletion of macrophages blocked the therapeutic effects of hUC-EVs-ATO on aGVHD. Mechanistically, hUC-EVs-ATO induced autophagic flux by inhibiting mammalian target of rapamycin (mTOR) activity to repolarize M1 to M2 macrophages. Additionally, using a murine model of GVL effects, hUC-EVs-ATO were found not only to reduce the severity of aGVHD but also to preserve the GVL effects. Taken together, hUC-EVs-ATO may be promising candidates for aGVHD treatment.

Conclusions: hUC-EVs-ATO enhanced the alleviation of aGVHD severity in mice compared with ATO and hUC-EVs without weakening GVL activity. hUC-EVs-ATO promoted M1 to M2 polarization via the mTOR-autophagy pathway. hUC-EVs-ATO could be a potential therapeutic approach in aGVHD after allo-HSCT.
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http://dx.doi.org/10.1186/s13045-022-01315-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306027PMC
July 2022

Dose-dependent early white matter alterations in patients with brain metastases after radiotherapy.

Neuroradiology 2022 Jul 22. Epub 2022 Jul 22.

Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People's Republic of China.

Purpose: Previous diffusion tensor imaging (DTI) studies have mainly focused on dose-dependent white matter (WM) alterations 1 month to 1 year after radiation therapy (RT) with a tract-average method. However, WM alterations immediately after RT are subtle, resulting in early WM alterations that cannot be detected by tract-average methods. Therefore, we performed a study with an along-tract method in patients with brain metastases to explore the early dose-response pattern of WM alterations after RT.

Methods: Sixteen patients with brain metastases underwent DTI before and 1-3 days after brain RT. DTI metrics, such as fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD) and mean diffusivity (MD), were calculated. Along-tract statistics were then used to resample WM fibre streamlines and generate a WM skeleton fibre tract. DTI metric alterations (post_RT-pre_RT DTI metrics) and the planned doses (max or mean doses) were mapped to 18 WM tracts. A linear fixed model was performed to analyse the main effect of dose on DTI metric alterations.

Results: AD alterations in the left hemispheric uncinated fasciculus (UNC_L) were associated with max doses, in which decreased AD alterations were associated with higher doses.

Conclusion: Our findings may provide pathological insight into early dose-dependent WM alterations and may contribute to the development of max dose-constrained RT techniques to protect brain microstructure in the UNC_L.
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http://dx.doi.org/10.1007/s00234-022-03020-wDOI Listing
July 2022

Polarized Molecule 4-(Aminomethyl) Benzonitrile Hydrochloride for Efficient and Stable Perovskite Solar Cells.

ACS Appl Mater Interfaces 2022 Jul 18. Epub 2022 Jul 18.

Research Center of Environment-Friendly Functional Materials, Ministry of Education, Institute of Materials Physical Chemistry, Huaqiao University, Xiamen 361021, China.

The rapid development of perovskite solar cells (PSCs) makes it one of the most competitive photovoltaic devices in the field of new energy. However, the suboptimal performance and poor stability caused by numerous defects are still the main factors limiting the development of PSCs. Herein, a polarized molecule additive of 4-(aminomethyl) benzonitrile hydrochloride (AMBNCl) is introduced into perovskite. Owing to its special polar electron density distribution, -C≡N group, -NH terminal, and Cl ions, the modification of AMBNCl can improve the quality of perovskite crystal growth, passivate the defects of Pb, adjust the energy level array between the perovskite layer and hole-transport layer, and alleviate the carrier nonradiative recombination. As a result, the AMBNCl-modified device achieves a champion efficiency of 23.52%. The unpacked device still maintained 91.2% of its original efficiency after storing in an air environment (RH ∼40%, 25 °C) for 50 days.
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http://dx.doi.org/10.1021/acsami.2c09058DOI Listing
July 2022

Effect of Coated Diammonium Phosphate Combined with Extracts on Soil Available Nutrients, Photosynthesis-Related Enzyme Activities, Endogenous Hormones, and Maize Yield.

ACS Omega 2022 Jul 29;7(27):23566-23575. Epub 2022 Jun 29.

National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Taian 271018, Shandong, China.

Coated diammonium phosphate (CDAP) is intended to release nutrients steadily in response to the demand of crop growth. A novel biostimulant extracted from has been shown to regulate gene expression in nutrient transport, enhance nitrogen (N) and phosphorus (P) uptake, and improve nutrient use efficiency. The application of CDAP combined with the extracts (ZNC) in maize is an efficient approach for reducing waste of resources, improving nutrient supply, and maintaining production stability. The effects of CDAP combined with ZNC on photosynthesis, enzyme activities, endogenous hormone content, maize yield, and P use efficiency (PUE) were investigated in this study. In a pot experiment, CDAP and diammonium phosphate (DAP) were tested together with P levels (1.80, 1.44 g pot, PO) and two ZNC application rates (0, 4.4 μg pot), which included the control treatment that had no P fertilizer added. Results showed that the key influencing elements of maize growth and yield were the soil available-P content, endogenous hormone content, and plant photosynthesis in this study. The combination of DAP and ZNC increased the soil available-P content and the auxin content in leaves at the key stage and hence increased the yield and PUE of maize, compared with DAP. The net photosynthetic rate of CDAP combined with ZNC was higher by 23.1% than that of CDAP alone, as well as by 32.0% than that of DAP combined with ZNC. Moreover, the combination of CDAP and ZNC increased the yield and PUE by 8.2% and 15.6 percentage points compared with DAP combined with ZNC while increasing the yield and PUE compared with CDAP. In conclusion, combining CDAP with ZNC as an environmentally friendly fertilizer could improve photosynthesis-related enzyme activity and enhance the net photosynthetic rate, resulting in an increase in maize yield and PUE significantly.
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http://dx.doi.org/10.1021/acsomega.2c02102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281325PMC
July 2022

Prediction of Newtonian Droplet Breaking Time from a Capillary at Low Weber Numbers.

ACS Omega 2022 Jul 28;7(27):23890-23898. Epub 2022 Jun 28.

Key Laboratory of Fluid Transmission Technology of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Droplet formation and growth processes have numerous scientific and industrial applications. Experimental and numerical studies on the formation, growth, and breaking of droplet are carried out in present work. The numerical results are in good agreement with the experiment. This work focused on the effect of different Weber numbers () on the droplet breaking time. The results show when < 0.05, the length and volume of the droplet increase, and the breaking time decreases rapidly. The resultant force acting on the main droplet suddenly drops around the critical breaking time. The difference rate between the time (when the resultant force is zero) and the breaking time is less than 8.49%. For the dimensional analysis of the numerical results, a prediction formula of breaking time on the Weber number is modeled as + for < 0.5.
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http://dx.doi.org/10.1021/acsomega.2c02602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280954PMC
July 2022

Safety and immunogenicity of an -produced bivalent human papillomavirus type 6/11 vaccine: A dose-escalation, randomized, double-blind, placebo-controlled phase 1 trial.

Hum Vaccin Immunother 2022 Jul 14:2092363. Epub 2022 Jul 14.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Strait Collaborative Innovation Center of Biomedicine and Pharmaceutics, Xiamen University, Xiamen, Fujian, China.

A dose-escalation, randomized, double-blind, placebo-controlled phase 1 clinical trial enrolled 145 eligible participants aged 18-55 years in March 2015 in Liuzhou, China. Stratified by age and sex, the participants were randomly assigned to receive either 30, 60, or 90 μg of the HPV-6/11 vaccine (n = 41/40/40) or the parallel placebo vaccine (n = 8/8/8) with a 0/1/6-month dose-escalation schedule. Participants were actively followed-up to record local and systemic AEs occurring within 30 days after each vaccination, and SAEs occurred in 7 months. Blood and urine samples of each participant were collected before and 2 days after the first and third vaccination to determine changes in routine blood, serum biochemical, and urine indexes. Serum HPV-6/11-specific IgG and neutralizing antibody levels at month 7 were analyzed. A total of 79 adverse events were reported, and no SAEs occurred. The incidences of total adverse reactions in the 30 μg, 60 μg, and 90 μg HPV vaccine groups and the control group were 31.7%, 50.0%, 42.5%, and 62.5%, respectively. All but one of the adverse reactions was mild or moderate with grade 1 or 2. No vaccine-related changes with clinical significance were found in paired blood and urine indexes before and after vaccinations. All the participants in the per-protocol set seroconverted at month 7 for both IgG and neutralizing antibodies. The candidate novel -produced bivalent HPV-6/11 vaccine has been preliminarily proven to be well tolerated and with robust immunogenicity in a phase 1 clinical study, supporting further trials with larger sample size. The study has been registered at ClinicalTrials.gov (NCT02405520).
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http://dx.doi.org/10.1080/21645515.2022.2092363DOI Listing
July 2022

Norepinephrine acting on adventitial fibroblasts stimulates vascular smooth muscle cell proliferation via promoting small extracellular vesicle release.

Theranostics 2022 6;12(10):4718-4733. Epub 2022 Jun 6.

Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, and Department of Physiology, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Excessive sympathetic activity and norepinephrine (NE) release play crucial roles in the pathogeneses of hypertension. Sympathetic fibers innervate adventitia rather than media of arteries. However, the roles of NE in adventitial fibroblasts (AFs) are unknown. This study investigated the roles of NE in regulating AFs-derived extracellular vesicles (EVs) release and vascular smooth muscle cells (VSMCs) proliferation in hypertension. AFs and VSMCs were prepared from aorta of Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). AFs were treated with NE (10 μM) for 24 h (every 6 h, 4 times), and cultured in exosomes-depleted medium for 48 h. EVs were isolated from AFs medium with ultracentrifugation for identification and transfer to VSMCs. NE promoted AFs phenotypic transformation and proliferation, which were prevented by α-receptor antagonist phentolamine rather than β-receptor antagonist propranolol. NE-treated AFs conditioned medium stimulated VSMCs proliferation, which was inhibited by either exosome inhibitor GW4869 or phentolamine. NE increased small EVs number, diameter and angiotensin converting enzyme (ACE) contents. The NE-induced EVs release was abolished by GW4869. The EVs from NE-treated AFs stimulated VSMCs proliferation, which was prevented by angiotensin II type 1 receptor antagonist losartan. The EVs from the ACE knockdown-treated AFs showed lower ACE contents, and lost their roles in stimulating VSMCs proliferation. NE promotes AFs-derived small EVs release and ACE transfer, and then causes VSMCs proliferation in hypertension. Intervention of AFs-derived EVs release may be potential therapeutics for excessive sympathetic activation-related vascular remodeling in hypertension.
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http://dx.doi.org/10.7150/thno.70974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254254PMC
June 2022

Segmentation and Recognition of the Pathological Features of Squamous Cell Carcinoma of the Skin Based on Multispectral Imaging.

J Clin Med 2022 Jul 1;11(13). Epub 2022 Jul 1.

Institute of Photomedicine, Shanghai Dermatology Hospital, School of Medicine, Tongji University, Shanghai 200443, China.

Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin cancers, a definitive diagnosis of cSCC is crucial to prevent patients from missing out on treatment. The gold standard for the diagnosis of cSCC is still pathological biopsy. Currently, its diagnostic efficiency and accuracy largely depend on the experience of pathologists. Here, we present a simple, fast, and robust technique, a microscopic multispectral imaging system based on LED illumination, to diagnose cSCC qualitatively and quantitatively. The adaptive threshold segmentation method was used to segment the multispectral images into characteristic structures. There was a statistically significant difference between the average nucleocytoplasmic ratio of normal skin (4.239%) and cSCC tissues (15.607%) ( < 0.01), and the keratin pearls cSCC have well-defined qualitative features. These results show that the qualitative and quantitative features obtained from multispectral imaging can be used to comprehensively determine whether or not the tissue is cancerous. This work has significant implications for the development of a low-cost and easy-to-use device, which can not only reduce the complexity of pathological diagnosis but can also achieve the goal of convenient digital staining and access to critical histological information.
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http://dx.doi.org/10.3390/jcm11133815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267474PMC
July 2022

External Validation of a Vancomycin Population Pharmacokinetic Model and Developing a New Dosage Regimen in Neonates.

Eur J Drug Metab Pharmacokinet 2022 Jul 8. Epub 2022 Jul 8.

Department of Neonatal and Developmental Medicine, Singapore General Hospital, Outram Road, Singapore, 169608, Singapore.

Background And Objective: Vancomycin is the drug of choice in the treatment of MRSA infections. In a published vancomycin population pharmacokinetic study on neonates in Singapore healthcare institutions, it was found that vancomycin clearance was predicted by weight, postmenstrual age, and serum creatinine. The aim of this study was to externally validate the vancomycin population pharmacokinetic model to develop a new dosage regimen in neonates, and to compare this regimen with the existing institutional and NeoFax dosage regimens.

Methods: A retrospective chart review of neonates who received vancomycin therapy and therapeutic drug monitoring was conducted. The median prediction error percentage was calculated to assess bias, while the median absolute prediction error percentage and the root mean squared error percentage were calculated to assess precision. The new dosage regimen was developed using Monte Carlo simulation.

Results: A total of 20 neonates were included in the external validation dataset. Eighteen of them were premature, with a median gestational age of 27.7 (25.9-31.5) weeks and postmenstrual age of 30.5 (27.3-34.3) weeks at the point of vancomycin initiation. No apparent systematic bias was found in the predictions of the model. The external validation performed in the current study found the model to be generally unbiased. Our new vancomycin dosage regimen was able to achieve target trough concentrations and area under the curve (AUC) at a greater proportion as compared to existing institutional and NeoFax dosage regimens.

Conclusion: The pharmacokinetic model built in the previous study can be used to conduct reliable population simulations of our Asian neonatal population in Singapore. The new dosage regimen was able to achieve target trough concentrations and AUC better than existing institutional and NeoFax dosage regimens.
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http://dx.doi.org/10.1007/s13318-022-00781-wDOI Listing
July 2022
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