Publications by authors named "Qi Chen"

2,357 Publications

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The local-neighborhood effect of green credit on green economy: a spatial econometric investigation.

Environ Sci Pollut Res Int 2021 Jul 28. Epub 2021 Jul 28.

School of Economics and Management, China University of Mining and Technology, No1, Daxue Road, Xuzhou, Jiangsu, 221116, People's Republic of China.

Green credit is one of the most important financial instruments to promote sustainable development. Taking the provincial panel dataset of China as the research sample, this paper investigates the spatial impacts of green credit on the green economy. The super slack-based measure (Sup-SBM) model with undesirable outputs is employed to calculate the level of green economy within China. On this basis, we establish spatial Durbin models to study the impact of green credit on green economy and its transmission mechanisms. The results show that green credit exhibits a local-neighborhood effect on green economy; that is, the green credit can not only improve the local green economy but also generate spatial spillover effect to promote the development of green economy in surrounding areas. The above conclusion still holds after the robustness test by replacing spatial weight matrices and alternative measurement for the explained variable. Furthermore, enhancing innovation efficiency and optimizing energy structure are important ways for green credit to promote green economy. The findings of this study not only provide a new perspective for understanding the economic consequences of green credit policy but also provide empirical evidence for the important role of green finance in achieving the win-win goals of economic growth and environmental protection.
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http://dx.doi.org/10.1007/s11356-021-15419-8DOI Listing
July 2021

Alginate-azo/chitosan nanocapsules in vitro drug delivery for hepatic carcinoma cells: UV-stimulated decomposition and drug release based on trans-to-cis isomerization.

Int J Biol Macromol 2021 Jul 24;187:214-222. Epub 2021 Jul 24.

College of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing 210000, PR China. Electronic address:

In this study, a nanocapsule (AL-azo/CH) was prepared with the anionic alginate-azo (AL-azo) and cationic chitosan (CH) via layer-by-layer method. Doxorubicin hydrochloride (DOX), an anticancer drug, was entrapped inside the AL-azo nanocapsules to form the DOX-loaded nanocapsules (DOX/AL-azo/CH). When the DOX/AL-azo/CH nanocapsules were irradiated with 365-nm light, the electrostatic attraction between the layers would be weakened as the trans-to-cis isomerization of AL-azo, which would lead to the UV-responsive decomposition and drug-release. Furthermore, cellular experiments showed that DOX/AL-azo/CH nanocapsules could be endocytosed by the HepG2 cells, and the confocal laser scanning microscope images showed that the DOX fluorescence intensity became stronger with the prolonging of irradiation time, indicating that the intracellular DOX-release could be controlled by UV irradiation. The AL-azo/CH nanocarriers were UV-triggered decomposition and drug-release, which stepped further towards the next-generation of nano-therapeutics with spatial and temporal external control in the field of polysaccharide.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.119DOI Listing
July 2021

Electrocatalytic water oxidation by a water-soluble copper complex with a pentadentate amine-pyridine ligand.

Dalton Trans 2021 Jul 26. Epub 2021 Jul 26.

Jiangsu Laboratory of Advanced Functional Materials, School of Materials Engineering, Changshu Institute of Technology, Changshu 215500, P. R. China.

A water-soluble copper complex with a diamine-tripyridine ligand was synthesized successfully and well characterized. It was found to be catalytically active for the water oxidation reaction under basic conditions. Based on the electrochemical test result, this copper complex displayed an apparent rate constant (kcat) of 0.81 s-1 for the oxygen evolution reaction in 0.1 M phosphate buffer solution at pH 11.0. More importantly, the copper complex remained stable over 3 h of a bulk electrolysis experiment at 1.60 V with a Faradaic efficiency of 90.7% for O2 evolution, and the decrement of current density was only 1.9%. These results suggest that the pentadentate copper complex is an efficient and durable homogeneous Earth-abundant electrocatalyst for water oxidation.
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http://dx.doi.org/10.1039/d1dt01821cDOI Listing
July 2021

Exploration of transmission chain and prevention of the recurrence of coronavirus disease 2019 in Heilongjiang Province due to in-hospital transmission.

World J Clin Cases 2021 Jul;9(20):5420-5426

Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.

The coronavirus disease 2019 (COVID-19) epidemic is a major public health emergency characterized by fast spread, a wide range of infections, and enormous control difficulty. Since the end of December 2019, Wuhan has become the first core infection area of China's COVID-19 outbreak. Since March 2020, the domestic worst-hit areas have moved to the Heilongjiang Province due to the increased number of imported COVID-19 cases. Herein, we reported the major COVID-19 outbreak, which caused a rebound of the epidemic in Harbin, China. After the rebound, different levels of causes for the recurrence of COVID-19, including city-level, hospital-level, and medical staff-level cause, were investigated. Meanwhile, corresponding countermeasures to prevent the recurrence of the epidemic were also carried out on the city level, hospital level, and medical staff level, which eventually showed the effect of infection control function in a pandemic. In this study, we described the complete transmission chain, analyzed the causes of the outbreak, and proposed corresponding countermeasures from our practical clinical experience, which can be used as a valuable reference for COVID-19 control.
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http://dx.doi.org/10.12998/wjcc.v9.i20.5420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281417PMC
July 2021

Body Mass Index and Disease Activity Are Associated With Moderate to Severe Disability in Crohn's Disease: A Cross-Sectional Study in Shanghai.

Front Med (Lausanne) 2021 9;8:662488. Epub 2021 Jul 9.

Department of Clinical Nutrition, School of Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.

The inflammatory bowel disease disability index (IBD-DI) was used to access body functional consequences and disease burden. However, Chinese population data are considerably limited. We aimed to screen for disability in patients with Crohn's disease (CD) and to assess potential associations with clinical parameters as well as indices related to sarcopenia. This cross-sectional study includes 146 CD patients from Ruijin Hospital in Shanghai, China. All patients were screened for disability and sarcopenia on the basis of the IBD-DI scale, and the criteria for Asian Working Group for Sarcopenia, respectively. Clinical and demographic variables were collected. Approximately 52.05% of the subjects suffered from moderate or severe disabilities. The prevalence of sarcopenia (48.68 vs. 31.43%, = 0.043), Patient-Generated Subjective Global Assessment score or PG-SGA≥4 (39.47 vs. 17.14%, = 0.003), and high-level C- reactive protein (27.63 vs. 11.43%, = 0.021) were higher in patients with moderate to severe disability than in those without to minimal disability. By multivariate regression modeling, the following were identified as independent factors related to moderate to severe disability: disease activity (OR:10.47, 95% CI: 2.09-52.42) and body mass index (BMI) (OR:4.11, 95% CI: 1.80-9.38). Disability is common in CD patients. Our study showed that moderate to severe disability is not directly associated with muscle mass or muscle quantity but is mostly correlated with disease activity as well as BMI. Thus, close monitoring and follow-up should be conducted on patients who are at high risk of disability, and effective measures should be taken, which may be the best way to prevent disability.
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http://dx.doi.org/10.3389/fmed.2021.662488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299001PMC
July 2021

Cumulative effects of ambient particulate matter pollution on deaths: A multicity analysis of mortality displacement.

Chemosphere 2021 Jul 20;286(Pt 1):131615. Epub 2021 Jul 20.

Jiangsu Provincial Center for Disease Control and Prevention, Jiangsu Road 172, 210009, Nanjing, PR China. Electronic address:

Background: Systematic evaluations of the cumulative effects and mortality displacement of ambient particulate matter (PM) pollution on deaths are lacking. We aimed to discern the cumulative effect profile of PM exposure, and investigate the presence of mortality displacement in a large-scale population.

Methods: We conducted a time-series analysis with different exposure-lag models on 13 cities in Jiangsu, China, to estimate the effects of PM pollution on non-accidental, cardiovascular, and respiratory mortality (2015-2019). Over-dispersed Poisson generalized additive models were integrated with distributed lag models to estimate cumulative exposure effects, and assess mortality displacement.

Results: Pooled cumulative effect estimates with lags of 0-7 and 0-14 days were substantially larger than those with single-day and 2-day moving average lags. For each 10 μg/m increment in PM concentration with a cumulative lag of 0-7 days, we estimated an increase of 0.50 % (95 % CI: 0.29, 0.72), 0.63 % (95 % CI: 0.38, 0.88), and 0.50 % (95 % CI: 0.01, 1.01) in pooled estimates of non-accidental, cardiovascular, and respiratory mortality, respectively. Both PM and PM were associated with significant increases in non-accidental and cardiovascular mortality with a cumulative lag of 0-14 days. We observed mortality displacement within 30 days for non-accidental, cardiovascular, and respiratory deaths.

Conclusions: Our findings suggest that risk assessment based on single-day or 2-day moving average lag structures may underestimate the adverse effects of PM pollution. The cumulative effects of PM exposure on non-accidental and cardiovascular mortality can last up to 14 days. Evidence of mortality displacement for non-accidental, cardiovascular, and respiratory deaths was found.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131615DOI Listing
July 2021

Influence of aging on chronic unpredictable mild stress-induced depression-like behavior in male C57BL/6J mice.

Behav Brain Res 2021 Jul 21:113486. Epub 2021 Jul 21.

Department of Applied Psychology, School of Humanities and Communication, Guangdong University of Finance & Economics, Guangzhou, 510320, China. Electronic address:

Depression is a common psychiatric disorder that can occur throughout an individual's lifespan. Chronic unpredictable mild stress (CUMS) protocol is currently the most commonly used to develop an animal model of depression. Due to the variable duration and procedure of CUMS, it is difficult to reproduce and explore the mechanism of CUMS-induced depression effectively. In the present study, the CUMS-induced behavioral phenotypes were assessed in male C57BL/6J mice at the age of 9-18 weeks. The mice stressed for 3-8 weeks exhibited lower body weight as well as longer immobility time of forced swim and tail suspension test compared to control mice. Moreover, lessening and impairment of hippocampal neurons was found in stressed mice at the age of 18 weeks, which was correlated with increased relative mRNA expression levels of inflammatory cytokines BDNF, Htr1a, and IL-6 in the hippocampus. Nevertheless, no difference between stressed and control mice was observed neither in the sucrose preference nor in the open field test (except for vertical activity in OFT) at the age of 18 weeks. These findings reveal that 3-8 weeks of chronic stress could induce depression-like alterations in male C57BL/6J mice and the behavioral adaptation of aged mice might fail to the availability of the depression model.
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http://dx.doi.org/10.1016/j.bbr.2021.113486DOI Listing
July 2021

Single-cell analyses reveal the differentiation shifts of Schwann cells in neonatal rat sciatic nerves.

J Cell Physiol 2021 Jul 20. Epub 2021 Jul 20.

Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu, China.

Schwann cells provide essential physical and chemical support for neurons and play critical roles in the peripheral nervous system. To acquire an enhanced understanding of the genetic characteristics of Schwann cells, we analyzed single-cell transcriptional profiling of Schwann cells in neonatal rat sciatic nerves, ordered the pseudotemporal states of Schwann cells, and determined the magnitude of RNA velocity vectors as well as cell cycle stages of Schwann cell subtypes. We discovered the cellular heterogeneity of Schwann cells in neonatal rat sciatic nerves, revealed the dynamic changes of Schwann cell subtypes, and pointed out the differentiation trajectory from Timp3- and Col5a3-expressing Schwann cell subtype 3 to other Schwann cell subtypes. The functional interpretation further indicated that subtype 3 Schwann cells display genetic signatures of DNA replication and the acquisition of mesenchymal traits. Our study presents a transcriptional summarization of the differentiation states of Schwann cell subtypes in neonatal rat sciatic nerves at single-cell resolution and may serve as a foundation for a deeper comprehension of the involvement of Schwann cells in the development and regeneration of peripheral nerves.
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http://dx.doi.org/10.1002/jcp.30533DOI Listing
July 2021

Changes in the Cytokine Profiles of Patients with Chronic Hepatitis B during Antiviral Therapy.

Biomed Environ Sci 2021 Jun;34(6):443-453

Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;Department of Hepatology Division 2, Peking University Ditan Teaching Hospital, Beijing 100015, China.

Objective: To investigate the changes in the cytokine profiles of chronic hepatitis B (CHB) patients undergoing antiviral treatment.

Methods: Hepatitis B e antigen (HBeAg)-positive patients were treated with Pegylated interferon (PEG-IFN) and entecavir (ETV). Clinical biochemistry and cytokines were detected at baseline and every 3 months.

Results: In all, 200 patients completed 48 weeks of treatment, 100 in the PEG-IFN group and 100 in the ETV group. During 3-6 months of treatment, compared with baseline, the PEG-IFN group showed a significant decrease in interferon-gamma (IFN-γ), interleukin-17A (IL-17A), interleukin-6(IL-6), interleukin-10(IL-10), and transforming growth factor beta (TGF-β) ( < 0.001) and a significant increase in interferon-alpha 2(IFN-α2) ( < 0.001). In the ETV group, IL-10 and TGF-β1 decreased significantly ( < 0.001). After 3 months, the levels of IFN-α2, IL-17A, and tumor necrosis factor-alpha(TNF-α) in the PEG-IFN group were significantly higher than those in the ETV group ( < 0.01). The levels of IL-6 and TGF-β3 were significantly lower than those in the ETV group ( < 0.01). After 6 months, the levels of IFN-α2, IFN-γ, and TNF-α in the PEG-IFN group were significantly higher than those in the ETV group ( < 0.01), while the levels of IL-6 and TGF-β3 were significantly lower than those in the ETV group ( < 0.01). Compared with ETV, PEG-IFN had higher HBeAg and HBsAg disappearance rates.

Conclusion: During antiviral therapy, a change in the cytokine profile occurred; in the aspect of immune control and functional cure, PEG-IFN was significantly better than ETV.
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http://dx.doi.org/10.3967/bes2021.061DOI Listing
June 2021

Solvent Free Laminated Fabrication of Lead Halide Perovskites for Sensitive and Stable X-ray Detection.

J Phys Chem Lett 2021 Jul 20;12(29):6961-6966. Epub 2021 Jul 20.

Sichuan Research Center of New Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Chengdu 610200, China.

The halide perovskite X-ray detector can meet the urgent needs of low-dose medical imaging by X-rays. However, there is still a pressing challenge in lacking robust methods for large-scale fabrication of high-quality perovskite films with tunable thickness. Here we report a laminated fabrication of polycrystalline MAPbI by using solvent-free liquid perovskite molten-salt (PMS), that offers reduced toxic issue, scalable fabrication, and highly tunability in film thickness. Nylon membrane was chosen as a scaffold for the infiltration of PMS, which simultaneously acts as a physical barrier to suppress the ionic migration in the MAPbI-nylon composite (denoted as MAPbI-LLP). The enhanced material properties result in good stability and high performance of X-ray detectors that show low detection limit and high sensitivity. Additionally, single gamma-ray photon detection was realized by MAPbI-LLP detectors. The promising performance characteristics of such polycrystalline detectors can accelerate the adoption of polycrystalline perovskites in X-ray imaging and gamma-ray detection.
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http://dx.doi.org/10.1021/acs.jpclett.1c02171DOI Listing
July 2021

PetM is Essential for the Stabilization and Function of the Cytochrome b6f Complex in Arabidopsis.

Plant Cell Physiol 2021 Jul 20. Epub 2021 Jul 20.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences/Institutes of Plant Physiology and Ecology, 300 Fenglin Road, Shanghai, 200032, PR China.

The cytochrome b6f (cyt b6f) acts as a center linker of electron transport between photosystem I and II in oxygenic photosynthesis. PetM, one of the eight subunits of the cyt b6f complex, is a small hydrophobic subunit at the outside periphery, the functional mechanism of which remains to be elucidated in higher plants. In this work, we found that unlike the PetM mutant in Synechocystis sp. PCC 6803, the Arabidopsis thaliana PetM mutant showed a bleached phenotype with yellowish leaves, block of photosynthetic electron transport and loss of photo-autotrophy, similar to the Arabidopsis PetC mutant. Although PetM is relatively conserved between higher plants and cyanobacteria, Synechocystis PetM could not rescue the PetM-knockout phenotype in Arabidopsis. We provide evidence that the Synechocystis PetM did not stably bind to the Arabidopsis cyt b6f complex. Based on these results, we suggest that PetM is required in Arabidopsis to maintain function of the cyt b6f complex, likely through its close link with core subunits to form a tight "fence" which stabilizes the core of the complex.
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http://dx.doi.org/10.1093/pcp/pcab116DOI Listing
July 2021

Do psychiatric diseases follow annual cyclic seasonality?

PLoS Biol 2021 Jul 19;19(7):e3001347. Epub 2021 Jul 19.

Committee on Genetics, Genomics and Systems Biology, The University of Chicago, Chicago, Illinois, United States of America.

Seasonal affective disorder (SAD) famously follows annual cycles, with incidence elevation in the fall and spring. Should some version of cyclic annual pattern be expected from other psychiatric disorders? Would annual cycles be similar for distinct psychiatric conditions? This study probes these questions using 2 very large datasets describing the health histories of 150 million unique U.S. citizens and the entire Swedish population. We performed 2 types of analysis, using "uncorrected" and "corrected" observations. The former analysis focused on counts of daily patient visits associated with each disease. The latter analysis instead looked at the proportion of disease-specific visits within the total volume of visits for a time interval. In the uncorrected analysis, we found that psychiatric disorders' annual patterns were remarkably similar across the studied diseases in both countries, with the magnitude of annual variation significantly higher in Sweden than in the United States for psychiatric, but not infectious diseases. In the corrected analysis, only 1 group of patients-11 to 20 years old-reproduced all regularities we observed for psychiatric disorders in the uncorrected analysis; the annual healthcare-seeking visit patterns associated with other age-groups changed drastically. Analogous analyses over infectious diseases were less divergent over these 2 types of computation. Comparing these 2 sets of results in the context of published psychiatric disorder seasonality studies, we tend to believe that our uncorrected results are more likely to capture the real trends, while the corrected results perhaps reflect mostly artifacts determined by dominantly fluctuating, health-seeking visits across a given year. However, the divergent results are ultimately inconclusive; thus, we present both sets of results unredacted, and, in the spirit of full disclosure, leave the verdict to the reader.
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http://dx.doi.org/10.1371/journal.pbio.3001347DOI Listing
July 2021

An adaptive learning method of anchor shape priors for biological cells detection and segmentation.

Comput Methods Programs Biomed 2021 Jul 8;208:106260. Epub 2021 Jul 8.

College of Computer Science and Technology, Zhejiang University of Technology, Hangzhou 310023, PR China; Key Laboratory of Visual Media Intelligent Processing Technology of Zhejiang Province, Hangzhou 310023, PR China. Electronic address:

Background And Objective: Owing to the variable shapes, large size difference, uneven grayscale and dense distribution among biological cells in an image, it is still a challenging task for the standard Mask R-CNN to accurately detect and segment cells. Especially, the state-of-the-art anchor-based methods fail to generate the anchors of sufficient scales effectively according to the various sizes and shapes of cells, thereby hardly covering all scales of cells.

Methods: We propose an adaptive approach to learn the anchor shape priors from data samples, and the aspect ratios and the number of anchor boxes can be dynamically adjusted by using ISODATA clustering algorithm instead of human prior knowledge in this work. To solve the identification difficulties for small objects owing to the multiple down-samplings in a deep learning-based method, a densification strategy of candidate anchors is presented to enhance the effects of identifying tinny size cells. Finally, a series of comparative experiments are conducted on various datasets to select appropriate a network structure and verify the effectiveness of the proposed methods.

Results: The results show that the ResNet-50-FPN combining the ISODATA method and densification strategy can obtain better performance than other methods in multiple metrics (including AP, Precision, Recall, Dice and PQ) for various biological cell datasets, such as U373, GoTW1, SIM+ and T24.

Conclusions: Our adaptive algorithm could effectively learn the anchor shape priors from the various sizes and shapes of cells. It is promising and encouraging for a real-world anchor-based detection and segmentation application of biomedical engineering in the future.
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http://dx.doi.org/10.1016/j.cmpb.2021.106260DOI Listing
July 2021

An Image Study on Local Anesthetic Spread in Healthy Volunteers Receiving Subcostal Exterior Semilunaris Transversus Abdominis Plane (SE-TAP) Block.

J Pain Res 2021 5;14:2013-2019. Epub 2021 Jul 5.

Department of Anesthesiology, The First Affiliated Hospital of Xiamen University, Xiamen, People's Republic of China.

Background: Despite the popularity of the ultrasound-guided transversus abdominis plane (TAP) block and the diversity of advancing approaches, the extent of injectate spread limits its clinical benefits. This study used three-dimensional computed tomography (3D-CT) imaging and a cold stimulus to evaluate the spread of a local anesthetic injected through the subcostal exterior semilunaris to transversus abdominis plane (SE-TAP) block in healthy volunteers.

Methods: Eight healthy volunteers received a right-side ultrasound-guided SE-TAP block with 25 mL of 0.3% ropivacaine. The sensory block was assessed by a cold stimulus at 30 min, 2 h, 4 h, and 8 h following the SE-TAP block according to the 18-zone division method. A CT scan and 3D imaging were performed after the first sensory assessment.

Results: The injectate spread into the transversus abdominis space in all eight volunteers. 3D imaging confirmed that the injectate spread extensively along the costal margin in the plane of the transverse abdominis muscle and that it surpassed the semilunaris. Regarding the assessment using cold stimulus, five of six anterior zones and three of six lateral zones obtained successful rates of cutaneous sensory block higher than 75% 30 min after SE-TAP. Sensory block was achieved in the ventral dermatomes of all volunteers.

Conclusion: Our study showed that the SE-TAP injectate, which was administered using simple anatomical landmarks to provide reliable analgesia for abdominal surgery, consistently spread along the costal margin and extensively blocked cutaneous sensitivity in the anterior and lateral abdominal walls.
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http://dx.doi.org/10.2147/JPR.S316582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274630PMC
July 2021

The Extracts of and from Oriental Medicinal Foods Regulate Inflammatory and Autophagic Pathways against Neural Injury after Ischemic Stroke.

Oxid Med Cell Longev 2021 26;2021:9663208. Epub 2021 Jun 26.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

The study indicates inflammation and autophagy are closely related to neural apoptosis in the pathology of ischemic stroke. In the study, we investigate the effects and mechanisms of the extracts of and (AC) from oriental medicinal foods on inflammatory and autophagic pathways in rat permanent middle cerebral artery occlusion model. Three doses of AC extract were, respectively, administered for 7 days. It suggests that AC extract treatment ameliorated scores of motor and sensory functions and ratio of glucose utilization in thalamic lesions in a dose-dependent manner. Expression of Iba1 was decreased and CD206 was increased by immunofluorescence staining, western blotting results showed expressions of TLR4, phosphorylated-IKK and IB, nuclear P65, NLRP3, ASC, and Caspase-1 were downregulated, and Beclin 1 and LC3 II were upregulated. Low concentrations of TNF-, IL-1, and IL-6 were presented by ELISA assay. Additionally, caspase 8 and cleaved caspase-3 expressions and the number of TUNEL positive cells in ipsilateral hemisphere were decreased, while the ratio of Bcl-2/Bax was increased. Simultaneously, in LPS-induced BV2 cells, it showed nuclear P65 translocation and secretion of proinflammatory cytokines were suppressed by AC extract-contained cerebrospinal fluid, and its intervened effects were similar to TLR4 siRNA treatment. Our study demonstrates that AC extract treatment attenuates inflammatory response and elevates autophagy against neural apoptosis, which contributes to the improvement of neurological function poststroke. Therefore, AC extract may be a novel neuroprotective agent by regulation of inflammatory and autophagic pathways for ischemic stroke treatment.
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http://dx.doi.org/10.1155/2021/9663208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257381PMC
June 2021

Transcriptome analysis reveals genes potentially related to high fiber strength in a line IL9 with introgression.

Genome 2021 Jul 12:1-11. Epub 2021 Jul 12.

School of Life Sciences, Nantong University, Nantong, Jiangsu 226019, P.R. China.

Cotton ( L.) is the most important fiber crop worldwide. Here, transcriptome analysis was conducted on developing fibers of a introgression line, IL9, and its recurrent parent, PD94042, at 17 and 21 days post-anthesis (dpa). Differentially expressed genes (DEGs) of PD94042 and IL9 were identified. Gene Ontology (GO) enrichment analysis showed that the annotated DEGs were rich in two main biological processes and two main molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis likewise showed that the annotated DEGs were mainly enriched in metabolic pathways and biosynthesis of secondary metabolites. In total, 52 DEGs were selected as candidate genes based on comparison of the DEGs and GO function annotation information. Quantitative real-time PCR (RT-qPCR) analysis results for 12 randomly selected DEGs were consistent with transcriptome analysis. SNP identification based on chromatin segment introgression showed that 394 SNPs were identified in 268 DEGs, and two genes with known functions were identified within fiber strength quantitative trait loci (QTL) regions or near the confidence intervals. We identified 52 key genes potentially related to high fiber strength in a introgression line and provided significant insights into the study of cotton fiber quality improvement.
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http://dx.doi.org/10.1139/gen-2020-0177DOI Listing
July 2021

High-Fat Diet Induced Gut Microbiota Alterations Associating With Ghrelin/Jak2/Stat3 Up-Regulation to Promote Benign Prostatic Hyperplasia Development.

Front Cell Dev Biol 2021 24;9:615928. Epub 2021 Jun 24.

Department of Urology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The role of high-fat diet (HFD) induced gut microbiota alteration and Ghrelin as well as their correlation in benign prostatic hyperplasia (BPH) were explored in our study. The gut microbiota was analyzed by 16s rRNA sequencing. Ghrelin levels in serum, along with Ghrelin and Ghrelin receptor in prostate tissue of mice and patients with BPH were measured. The effect of Ghrelin on cell proliferation, apoptosis, and induction of BPH in mice was explored. Our results indicated that BPH mice have the highest ratio of Firmicutes and Bacteroidetes induced by HFD, as well as Ghrelin level in serum and prostate tissue was significantly increased compared with control. Elevated Ghrelin content in the serum and prostate tissue of BPH patients was also observed. Ghrelin promotes cell proliferation while inhibiting cell apoptosis of prostate cells. The effect of Ghrelin on enlargement of the prostate was found almost equivalent to that of testosterone propionate (TP) which may be attenuated by Ghrelin receptor antagonist YIL-781. Ghrelin could up-regulate Jak2/pJak2/Stat3/pStat3 expression and . Our results suggested that Gut microbiota may associate with Ghrelin which plays an important role in activation of Jak2/Stat3 in BPH development. Gut microbiota and Ghrelin might be pathogenic factors for BPH and could be used as a target for mediation.
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http://dx.doi.org/10.3389/fcell.2021.615928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264431PMC
June 2021

Quantitative dynamic contrast-enhanced MRI and readout segmentation of long variable echo-trains diffusion-weighted imaging in differentiating parotid gland tumors.

Neuroradiology 2021 Jul 9. Epub 2021 Jul 9.

Department of Radiology, First Affiliated Hospital of Fujian Medical University, 20 Cha-Zhong Road, Fuzhou, Fujian, 350005, People's Republic of China.

Purpose: To evaluate the ability of quantitative dynamic contrast-enhanced (DCE)-MRI and readout segmentation of long variable echo-trains diffusion-weighted imaging (RESOLVE-DWI) in differentiating parotid tumors (PTs) with different histological types.

Methods: In this retrospective study, 123 patients with 145 histologically proven PTs who underwent both RESOLVE-DWI and DCE-MRI were enrolled including 51 pleomorphic adenomas (PAs), 52 Warthin's tumors (WTs), 27 other benign neoplasms (OBNs), and 15 malignant tumors (MTs). Quantitative parameters of DCE-MRI (K, K, and V) and the apparent diffusion coefficient (ADC) of lesions were calculated and analyzed. Kruskal-Wallis tests with Dunn-Bonferroni correction, logistic regression analyses, and receiver operating characteristic curve were used for statistical analyses.

Results: PAs exhibited a lowest K among these four PTs. WTs demonstrated the highest K and lowest V values. WTs and MTs showed lower ADC values than PAs and OBNs. The combination of K and V provided 98.1% sensitivity, 85% specificity, and 98.7% accuracy for differentiating WTs from the other three PTs. The ADC cutoff value of ≤ 0.826 yielded 80.0% sensitivity, 92.3% specificity, and 90.3% accuracy for the differentiation of MTs from PAs and OBNs. K with a cutoff value of ≤ 0.185 achieved a sensitivity, specificity, and accuracy of 84.3, 70.4, and 79.5%, respectively, for discriminating PAs from OBNs.

Conclusion: The combination of quantitative DCE-MRI and RESOLVE-DWI is beneficial for characterizing four histological types of PTs.
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http://dx.doi.org/10.1007/s00234-021-02758-zDOI Listing
July 2021

Development of a Ferroptosis-Related lncRNA Signature to Predict the Prognosis and Immune Landscape of Bladder Cancer.

Dis Markers 2021 20;2021:1031906. Epub 2021 Jun 20.

Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China.

The tight relationship between ferroptotic cell death and immune response demonstrated by recent studies enlightened us to detect the underlying roles of ferroptosis-related long noncoding RNAs (frlncRNAs) in the tumor microenvironment of bladder cancer (BCa). We collected 121 ferroptosis regulators from previous studies. Based on their expression values, 408 cases with BCa were clustered. The patients in different clusters showed diverse immune infiltration, immunotherapy response, and chemotherapy effectiveness, revalidating the tight correlation with ferroptosis and tumor immunity. Through differential, coexpression, Kaplan-Meier, Lasso, and Cox analysis, we developed a 22-lncRNA-pair signature to predict the prognosis of BCa based on gene-pair strategy, where there is no need for definite expression values. The areas under the curves are all over 0.8. The risk model also helped to predict immune infiltration, immunotherapeutic outcomes, and chemotherapy sensitivity. Totally, the prognostic assessment model indicated a promising predictive value, also providing clues for the interaction between ferroptosis and BCa immunity.
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http://dx.doi.org/10.1155/2021/1031906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238626PMC
June 2021

A clinical prediction model identifies a subgroup with inferior survival within intermediate risk acute myeloid leukemia.

J Cancer 2021 11;12(16):4912-4923. Epub 2021 Jun 11.

Department of Hematology, Institute of Hematology, Changhai Hospital, Shanghai 200433, China.

Intermediate risk acute myeloid leukemia (AML) comprises around 50% of AML patients and is featured with heterogeneous clinical outcomes. The study aimed to generate a prediction model to identify intermediate risk AML patients with an inferior survival. We performed targeted next generation sequencing analysis for 121 patients with 2017 European LeukemiaNet-defined intermediate risk AML, revealing 122 mutated genes, with 24 genes mutated in > 10% of patients. A prognostic nomogram characterized by white blood cell count ≥10×10/L at diagnosis, mutated and genes involved in signaling pathways was developed for 110 patients who were with clinical outcomes. Two subgroups were identified: intermediate low risk (ILR; 43.6%, 48/110) and intermediate high risk (IHR; 56.4%, 62/110). The model was prognostic of overall survival (OS) and relapse-free survival (RFS) (OS: Concordance index [C-index]: 0.703, 95%CI: 0.643-0.763; RFS: C-index: 0.681, 95%CI 0.620-0.741), and was successfully validated with two independent cohorts. Allogeneic hematopoietic stem cell transplantation (alloHSCT) reduced the relapse risk of IHR patients (3-year RFS: alloHSCT: 40.0±12.8% chemotherapy: 8.6±5.8%, = 0.010). The prediction model can help identify patients with an unfavorable prognosis and refine risk-adapted therapy for intermediate risk AML patients.
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http://dx.doi.org/10.7150/jca.57231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247394PMC
June 2021

An Isolable Mononuclear Palladium(I) Amido Complex.

J Am Chem Soc 2021 Jul 7;143(28):10751-10759. Epub 2021 Jul 7.

State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.

Mononuclear Pd(I) species are putative intermediates in Pd-catalyzed reactions, but our knowledge about them is limited due to difficulties in accessing them. Herein, we report the isolation of a Pd(I) amido complex, [(BINAP)Pd(NHAr)] (BINAP = 2,2'-bis(diphenylphosphino)-1,1'-binaphthalene, Ar = 2,6-bis(2',4',6'-triisopropylphenyl)phenyl), from the reaction of (BINAP)PdCl with LiNHAr. This Pd(I) amido species has been characterized by X-ray crystallography, electron paramagnetic resonance, and multiedge Pd X-ray absorption spectroscopy. Theoretical study revealed that, while the three-electron-two-center π-interaction between Pd and N in the Pd(I) complex imposes severe Pauli repulsion in its Pd-N bond, pronounced attractive interligand dispersion force aids its stabilization. In accord with its electronic features, reactions of homolytic Pd-N bond cleavage and deprotonation of primary amines are observed on the Pd(I) amido complex.
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http://dx.doi.org/10.1021/jacs.1c04965DOI Listing
July 2021

SARS-CoV-2 infection in the mouse olfactory system.

Cell Discov 2021 Jul 6;7(1):49. Epub 2021 Jul 6.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in humans, and olfactory dysfunction is one of the most predictive and common symptoms in COVID-19 patients. However, the underlying mechanism by which SARS-CoV-2 infection leads to olfactory disorders remains elusive. Herein, we demonstrate that intranasal inoculation with SARS-CoV-2 induces robust viral replication in the olfactory epithelium (OE), not the olfactory bulb (OB), resulting in transient olfactory dysfunction in humanized ACE2 (hACE2) mice. The sustentacular cells and Bowman's gland cells in the OE were identified as the major target cells of SARS-CoV-2 before invasion into olfactory sensory neurons (OSNs). Remarkably, SARS-CoV-2 infection triggers massive cell death and immune cell infiltration and directly impairs the uniformity of the OE structure. Combined transcriptomic and quantitative proteomic analyses revealed the induction of antiviral and inflammatory responses, as well as the downregulation of olfactory receptor (OR) genes in the OE from the infected animals. Overall, our mouse model recapitulates olfactory dysfunction in COVID-19 patients and provides critical clues for understanding the physiological basis for extrapulmonary manifestations of COVID-19.
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http://dx.doi.org/10.1038/s41421-021-00290-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260584PMC
July 2021

Senescent Changes and Endoplasmic Reticulum Stress May Be Involved in the Pathogenesis of Missed Miscarriage.

Front Cell Dev Biol 2021 17;9:656549. Epub 2021 Jun 17.

Department of Obstetrics and Gynaecology, The University of Auckland, Auckland, New Zealand.

Background: Senescence is involved in many complications of pregnancy. However, whether senescent changes are also associated with missed miscarriage has not been fully investigated.

Methods: The levels of p16, p21, and γH2AX, markers of senescence, were measured in placentas collected from women with missed miscarriage by immunohistochemistry and Western blotting. Levels of misfolded proteins in missed miscarriage placentas or normal first-trimester placenta that had been treated with HO (100 μM) or extracellular vesicles (EVs) collected from missed miscarriage placental explant culture were measured by fluorescent compound, thioflavin-T. The production of reactive oxygen species (ROS) by missed miscarriage placentas was measured by CellROX Deep Red.

Results: Increased levels of p16, p21, and γH2AX were presented in missed miscarriage placentas compared to controls. Increased levels of misfolded proteins were shown in missed miscarriage placentas, but not in EVs that were collected from missed miscarriage placentas. The ROS production was significantly increased in missed miscarriage placental explant cultures. Increased levels of misfolded proteins were seen in the normal first-trimester placenta that had been treated with HO compared to untreated.

Conclusion: Our data demonstrate that there are increases in senescence and endoplasmic reticulum stress and ROS production in missed miscarriage placenta. Oxidative stress and an accumulation of misfolded proteins in missed miscarriage placentas may contribute to the changes of senescence and endoplasmic reticulum stress seen in missed miscarriage placentas.
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http://dx.doi.org/10.3389/fcell.2021.656549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247570PMC
June 2021

Modified 5-aminolevulinic acid photodynamic therapy (M-PDT) inhibits cutaneous squamous cell carcinoma cell proliferation via targeting PP2A/PP5-mediated MAPK signaling pathway.

Int J Biochem Cell Biol 2021 Aug 2;137:106036. Epub 2021 Jul 2.

Institute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China. Electronic address:

Background: We previously demonstrated that M-PDT is painless and effective in precancerous skin diseases treatment. However, whether M-PDT is effective in cSCC and the underlying inhibitory mechanism remains enigmatic.

Objective: We aims to unveil the effect of M-PDT on cSCC cell proliferation and the regulatory effect of M-PDT on MAPK signaling.

Methods: The proliferation and migration of cSCC cells were revealed by CCK8 assay, tumor sphere formation assay and scratch assay respectively. The expression of MAPKs was examined by western blot. The activity of PP2A and PP5 was regulated by inhibitor and recombinant adenoviruses.

Results: Here, we show that M-PDT inhibits cSCC cell proliferation by activating p-JNK, p-p38 and inhibiting p-Erk1/2, as well as activation of PP2A and inactivation of PP5. Furthermore, pharmacological inhibition of PP2A conferred resistance to M-PDT's suppression on p-Erk1/2 and attenuated inhibitory effects of M-PDT on cell proliferation whereas overexpression of wild-type PP2A showed the contrary results. Pharmacological inhibition of PP5 potentiated M-PDT's elevation on p-JNK and strengthened inhibitory effects of M-PDT on cell proliferation whereas overexpression of wild-type PP5 exhibited the contrary results.

Conclusion: Our findings indicate that M-PDT inhibits cSCC cell proliferation via targeting PP2A/PP5-mediated MAPK signaling pathway.
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http://dx.doi.org/10.1016/j.biocel.2021.106036DOI Listing
August 2021

Melatonin increases leaf disease resistance and saponin biosynthesis in Panax notogiseng.

J Plant Physiol 2021 Aug 26;263:153466. Epub 2021 Jun 26.

Laboratory of Sustainable Utilization of Panax notoginseng Resources, State Administration of Traditional Chinese Medicine, Key Laboratory of Panax notoginseng in Yunnan Province, Panax notoginseng Research Institute in Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China. Electronic address:

Panax notoginseng (Bruk.) FH Chen is a valuable traditional herb in China, with saponins being the main medicinal components in its roots. However, leaf diseases are a major factor limiting growth and production of P. notoginseng. Melatonin is a ubiquitous signaling molecule associated with abiotic stress resistance. In this study, we investigated the role of melatonin in leaf disease resistance of P. notoginseng in field conditions. Additionally, saponin concentrations were analyzed to evaluate the suitability of melatonin use in agricultural practice. Our results showed that exogenous application of melatonin promoted the endogenous phytomelatonin accumulation via upregulation of genes involved in its biosynthesis. The application of 10 μM melatonin decreased the incidence of leaf diseases (gray mold, round spot, and black spot) by about 40% compared with the solvent control, which might have been due to the increased expression of genes associated with immunity and disease resistance. Furthermore, concentrations of saponins and expression of their biosynthesis-related genes were significantly increased by melatonin. Taken together, the data presented here suggested that melatonin could be used in agricultural management of P. notoginseng because it increased leaf disease resistance and biosynthesis of saponins.
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http://dx.doi.org/10.1016/j.jplph.2021.153466DOI Listing
August 2021

ATF3 Modulates the Resistance of Breast Cancer Cells to Tamoxifen through an -Methyladenosine-Based Epitranscriptomic Mechanism.

Chem Res Toxicol 2021 Jul 2;34(7):1814-1821. Epub 2021 Jul 2.

Tamoxifen has been used for years for treating estrogen receptor-positive breast cancer; drug resistance, however, constitutes one of the main challenges for this therapy. We found that the protein expression level of ATF3 is significantly higher in tamoxifen-resistant (TamR) MCF-7 cells than the corresponding parental cancer cells. In addition, ATF3 protein expression is positively correlated with the resistance of TamR MCF-7 cells to 4-hydroxytamoxifen (4-OHT). Mechanistically, elevated ATF3 protein expression in TamR MCF-7 cells results from a lower level of expression of YTHDF2, an mA reader protein, and the ensuing stabilization and increased translational efficiency of ATF3 mRNA. Additionally, TamR MCF-7 cells exhibited decreased methylation at A131, a consensus motif site for mA, in the 5'-untranslated region (5'-UTR) of ATF3 mRNA. Moreover, augmented ATF3 stimulates the expression of ABCB1, an efflux pump that confers drug resistance in breast cancer cells, and ATF3 itself is also positively regulated by adenylate kinase 4. Together, our results uncovered a novel molecular target for mA modification (i.e., ATF3 mRNA) and the epitranscriptomic regulator for this target (i.e., YTHDF2). We also illustrated the role of ATF3 in drug resistance, revealed its downstream target (i.e., ABCB1), and suggested ATF3 as a candidate therapeutic target for overcoming drug resistance in cancer cells.
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http://dx.doi.org/10.1021/acs.chemrestox.1c00206DOI Listing
July 2021

A Comparison of Treatment Options for Type 1 and Type 2 Caesarean Scar Pregnancy: A Retrospective Case Series Study.

Front Med (Lausanne) 2021 15;8:671035. Epub 2021 Jun 15.

Department of Obstetrics and Gynaecology, The University of Auckland, Auckland, New Zealand.

There is currently no agreement on the optimal management of caesarean scar pregnancy. Caesarean scar pregnancy is currently categorised into two subtypes according to the site of implantation. This may consequently result in the difference in treatment options. However, the comparison of the success rate of each treatment option according to the subtypes has not been fully investigated. 71 patients who were treated by uterine curettage (D and C), or uterine artery embolization with curettage (UAE) or hysteroscopy in conjunction with laparoscopy between January 2016 and March 2020 were included. Data on maternal age, gestational sac age, the sac diameter, the interval between two pregnancies, the number of previous caesarean sections, amount of bleeding and β-hCG levels were collected and analysed dependent on the subtypes. There was no difference in the clinical parameters of the cases who received different options of treatment, as well as no difference in the clinical parameters between type 1 and type 2 caesarean scar pregnancy. The primary success rate for type 1 caesarean scar pregnancy by D and C, or UAE, or hysteroscopy in conjunction with laparoscopy was 95, or 100 or 100%, respectively. The primary success rate for type 2 caesarean scar pregnancy by D and C, or UAE, or hysteroscopy in conjunction with laparoscopy was 27, or 67, or 95% respectively. Our data demonstrates that hysteroscopy in conjunction with laparoscopy for type 2 caesarean scar pregnancy was the most successful compared to other options, but for type 1 caesarean scar pregnancy, D and C could be the cost-effective option.
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http://dx.doi.org/10.3389/fmed.2021.671035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240870PMC
June 2021

The Effect of Comorbid Attention-Deficit/Hyperactivity Disorder Symptoms on Face Memory in Children with Autism Spectrum Disorder: Insights from Transdiagnostic Profiles.

Brain Sci 2021 Jun 28;11(7). Epub 2021 Jun 28.

Department of Maternal and Child Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.

Face memory impairments are common but heterogeneous in autism spectrum disorder (ASD), which may be influenced by co-occurrence with attention-deficit/hyperactivity disorder (ADHD). Here, we aimed to investigate the phenotype change of face memory in children with ASD comorbid ADHD symptoms, and discuss the potential role of executive function (EF). Ninety-eight children were analyzed in the present study, including ASD- (ASD-only, n = 24), ADHD (n = 23), ASD+ (with ADHD symptoms, n = 23) and neurotypical controls (NTC, n = 28). All participants completed two tests: face encoding and retrieving task and Wisconsin Card Sorting Test (WCST) for measuring face memory and EF, respectively. Results revealed that: compared with the NTC group, children with ASD- exhibited lower accuracy in both face encoding and retrieving, and participants with ASD+ showed lower accuracy only in the retrieving, whereas no differences were found among participants with ADHD. Moreover, in the ASD+ group, face encoding performance was correlated with response perseverative errors (RPE) and failure to maintain sets (FMS) of WCST; significantly, there were no group differences between ASD+ and NTC in these two indices. The transdiagnostic profiles indicated that comorbid ADHD symptoms could modulate the face encoding deficiency of ASD, which may be partially compensated by EF. Shared and distinct intervention strategies to improve social cognition are recommended for children undergoing treatment for each condition.
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http://dx.doi.org/10.3390/brainsci11070859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301798PMC
June 2021

Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from 'Chachiensis'.

Microorganisms 2021 Jun 4;9(6). Epub 2021 Jun 4.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

'Chachiensis' (PCR-Chachiensis), the pericarps of Blanco cv. Chachiensis, is a food condiment and traditional medicine in southeast and eastern Asia. Its rich and various bacterial community awaits exploration. The present study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis. Based on 64 culturable bacterial isolates, 8 strains were screened out to have great survival in the simulated gastrointestinal stressful condition, being nonhemolytic and without biogenic amine formation. They were identified by 16S rRNA gene sequencing as two , three , and three strains from Bacillales. Their probiotic properties, cholesterol-lowering potential and carbohydrate utilization capability were further investigated. Though these eight strains all displayed distinct cholesterol removal potential, N17-02 showed both remarkable cholesterol removal capability and presence of bile salt hydrolase gene, as well as possessing most of the desirable probiotic attributes. Thus, it could be a good probiotic candidate with hypocholesterolemic potential. N17-12 displayed the widest carbohydrate utilization profile and the strongest antimicrobial activity. Hence, it was promising to be used as a probiotic in a host and as a fermentation starter in fermented food or feed.
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http://dx.doi.org/10.3390/microorganisms9061224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227569PMC
June 2021
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