Publications by authors named "Qi Cui"

204 Publications

Learning from Black/African American Participants: Applying the Integrated Behavioral Model to Assess Recruitment Strategies for a Glaucoma Genetic Study.

Health Commun 2020 Dec 19:1-10. Epub 2020 Dec 19.

Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania.

The underrepresentation of African American (AA) participants in medical research perpetuates racial health disparities in the United States. Open-ended phone interviews were conducted with 50 AA adults from Philadelphia who had previously participated in a genetic study of glaucoma that included complimentary ophthalmic screenings. Recruitment for the genetic study was done in partnership with a Black-owned radio station. Thematic analysis of interview transcripts, guided by the integrated behavior model (IBM), identified self-reported motivations for participating in this care-focused and community-promoted research program. Findings revealed that decisions to enroll were influenced by strong instrumental attitudes regarding learning more about personal health and contributing to future care options for others. Notable normative influences that factored into participants' decisions to enroll in the study included hearing about the study from a respected community media outlet, friends, and family. About one-third of respondents discussed past and current racial discrimination in medical research as an important sociocultural frame within which they thought about participation, suggesting that experiential attitudes play a continuing role in AA's decisions to enroll in medical research studies. Medical researchers seeking to recruit AA participants should collaborate with community partners, combine enrollment opportunities with access to health services, and emphasize the potential for new research to mitigate racial inequalities.
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http://dx.doi.org/10.1080/10410236.2020.1853897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213868PMC
December 2020

Cell-Based Therapy for Canavan Disease Using Human iPSC-Derived NPCs and OPCs.

Adv Sci (Weinh) 2020 Dec 29;7(23):2002155. Epub 2020 Oct 29.

Division of Stem Cell Biology Research Department of Developmental and Stem Cell Biology Beckman Research Institute of City of Hope 1500 E. Duarte Rd. Duarte CA 91010 USA.

Canavan disease (CD) is a fatal leukodystrophy caused by mutation of the aspartoacylase () gene, which leads to deficiency in ASPA activity, accumulation of the substrate N-acetyl--aspartate (NAA), demyelination, and spongy degeneration of the brain. There is neither a cure nor a standard treatment for this disease. In this study, human induced pluripotent stem cell (iPSC)-based cell therapy is developed for CD. A functional gene is introduced into patient iPSC-derived neural progenitor cells (iNPCs) or oligodendrocyte progenitor cells (iOPCs) via lentiviral transduction or TALEN-mediated genetic engineering to generate ASPA iNPC or ASPA iOPC. After stereotactic transplantation into a CD (Nur7) mouse model, the engrafted cells are able to rescue major pathological features of CD, including deficient ASPA activity, elevated NAA levels, extensive vacuolation, defective myelination, and motor function deficits, in a robust and sustainable manner. Moreover, the transplanted mice exhibit much prolonged survival. These genetically engineered patient iPSC-derived cellular products are promising cell therapies for CD. This study has the potential to bring effective cell therapies, for the first time, to Canavan disease children who have no treatment options. The approach established in this study can also benefit many other children who have deadly genetic diseases that have no cure.
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http://dx.doi.org/10.1002/advs.202002155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709977PMC
December 2020

Serine protease inhibitor MDSPI16 ameliorates LPS-induced acute lung injury through its anti-inflammatory activity.

Int Immunopharmacol 2020 Nov 22;88:107015. Epub 2020 Sep 22.

College of Life Science, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China. Electronic address:

A previous study described a novel serine protease inhibitor 16 from Musca domestica (MDSPI16), which inhibited the elastase and chymotrypsin. It also exhibited a potential anti-inflammatory activity for acute lung injury (ALI), while its effects on ALI are yet to be elucidated. The present study aimed to investigate the effects and the underlying mechanisms of MDSPI16 on lipopolysaccharide (LPS)-challenged mice and bone marrow neutrophils. The ALI model based on the results of LPS-induced mice demonstrated that MDSPI16 markedly reduced the infiltration of inflammatory cells, protein exudation in lung tissues, and downregulated the level of interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α). Furthermore, the LPS-stimulated mouse bone marrow neutrophils model was employed to determine the role of MDSPI16. The cytokine levels were quantified by both the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Consequently, the expression of IL-6, IL-1β, and TNF-α was found to be inhibited by MDSPI16 in a dose-dependent manner. Moreover, MDSPI16 also inhibited the mouse neutrophils nuclear factor-κB (NF-κB) signaling pathway, c-Jun N-terminal kinase (JNK) signaling pathway, ERK1/2 and AP-1 signaling pathway in addition to the expression of iNOS and COX-2 proteins, which in turn, might alleviate the release of pro-inflammatory cytokines during ALI. Therefore, MDSPI16 could be proposed as a potential and novel drug therapy for ALI.
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http://dx.doi.org/10.1016/j.intimp.2020.107015DOI Listing
November 2020

Investigation of viral pathogens in cattle with bovine respiratory disease complex in Inner Mongolia, China.

Microb Pathog 2021 Apr 4;153:104594. Epub 2020 Nov 4.

College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, China. Electronic address:

As a multifactor disease, the bovine respiratory disease complex (BRDC) causes high morbidity and mortality that is devastating to the cattle industry. To assess viral infections in beef cattle suffering from respiratory diseases in Inner Mongolia, 302 nasal swabs and serum samples were randomly collected from cattle with mild respiratory symptoms between March 2018 and May 2019. Our results showed that the rate of RT-PCR results positive for nucleic acids of viral pathogens in 6 cities was between 54 and 80%.The rates of bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus type 3(BPIV3), and bovine respiratory syncytial virus(BRSV)infections were 44.70% (135/302), 24.83% (75/302), 5.63% (17/302), and 6.95% (21/302),respectively. There are also 8.94% (27/302) of samples were positive for BVDV and BHV-1, and 3.97% (12/302) of samples were positive for BPIV3 and BRSV. In addition, the RT-PCR products were sequenced, and phylogenetic analysis based on these sequences was performed. The results indicated that: a) all of the BVDV isolates were BVDV-1 and were classified as BVDV-1a (66.67%) and BVDV-1b (33.33%); b) all of the BHV-1 isolates were classified as subtype 1.1; 44.44% of the isolates were closely related to modified live viral vaccine strains, and 55.56% of the isolates were closer to epidemic strains; c) all of the BPIV3 isolates belonged to BPIV3c; d) all of the BRSV isolates were classified into subgroup III. It is suggested that an important cause of respiratory diseases for beef cattle is viral infection, and phylogenetic analysis can help us choose the proper strain to develop a vaccine.
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http://dx.doi.org/10.1016/j.micpath.2020.104594DOI Listing
April 2021

Full-field spectral-domain optical interferometry for snapshot three-dimensional microscopy.

Biomed Opt Express 2020 Oct 28;11(10):5903-5919. Epub 2020 Sep 28.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Prevalent techniques in label-free linear optical microscopy are either confined to imaging in two dimensions or rely on scanning, both of which restrict their applications in imaging subtle biological dynamics. In this paper, we present the theoretical basis along with demonstrations supporting that full-field spectral-domain interferometry can be used for imaging samples in 3D with no moving parts in a single shot. Consequently, we propose a novel optical imaging modality that combines low-coherence interferometry with hyperspectral imaging using a light-emitting diode and an image mapping spectrometer, called Snapshot optical coherence microscopy (OCM). Having first proved the feasibility of Snapshot OCM through theoretical modeling and a comprehensive simulation, we demonstrate an implementation of the technique using off-the-shelf components capable of capturing an entire volume in 5 ms. The performance of Snapshot OCM, when imaging optical targets, shows its capability to axially localize and section images over an axial range of ±10 µm, while maintaining a transverse resolution of 0.8 µm, an axial resolution of 1.4 µm, and a sensitivity of up to 80 dB. Additionally, its performance in imaging weakly scattering live cells shows its capability to not only localize the cells in a densely populated culture but also to generate detailed phase profiles of the structures at each depth for long durations. Consolidating the advantages of several widespread optical microscopy modalities, Snapshot OCM has the potential to be a versatile imaging technique for a broad range of applications.
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http://dx.doi.org/10.1364/BOE.402796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587259PMC
October 2020

GLP-1 Receptor Agonist NLY01 Reduces Retinal Inflammation and Neuron Death Secondary to Ocular Hypertension.

Cell Rep 2020 11;33(5):108271

FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA. Electronic address:

Glaucoma is the leading cause of irreversible blindness and is characterized by the death of retinal ganglion cells (RGCs). Recent studies have implicated pro-inflammatory microglia, macrophages, and A1 astrocytes in the pathogenesis of neurodegenerative diseases. The role of pro-inflammatory, neurotoxic A1 astrocytes in glaucoma is just beginning to be explored. Using a mouse model of glaucoma, we demonstrate that ocular hypertension is sufficient to trigger production of C1q, interleukin-1α (IL-1α), and tumor necrosis factor α (TNF-α), three cytokines necessary and sufficient to drive the formation of A1 astrocytes. Upregulation of these cytokines occurs first in CD11b CD11c cells followed by CD11b CD11c cells. Ablation of this pathway, by either genetic deletions of C1qa, IL-1α, and TNF-α, or treatment with glucagon-like peptide-1 receptor agonist NLY01, reduces A1 astrocyte transformation and RGC death. Together, these results highlight a neuroinflammatory mechanism of glaucomatous neurodegeneration that can be therapeutically targeted by NLY01 administration.
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http://dx.doi.org/10.1016/j.celrep.2020.108271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660987PMC
November 2020

Preparation, Characterization and Pharmacokinetic Study of N-Terminal PEGylated D-Form Antimicrobial Peptide OM19r-8.

J Pharm Sci 2021 03 28;110(3):1111-1119. Epub 2020 Oct 28.

College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.2888, Changchun 130118, China; The Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Xincheng Street No.2888, Changchun, Jilin, China. Electronic address:

Recently, new cationic antibacterial peptide OM19R has been designed with low minimum inhibitory concentration (MIC) values against some gram-negative bacteria, such as Escherichia coli, Salmonella, and Shigella. However, this hybrid peptide, like most antibacterial peptides, has low enzyme stability and short half-life, which, in turn, increases the drug's cost. In this study, an antibacterial peptide (OM19r-8) was obtained containing some D-Arg amino acids. The new preparations were carried out through the replacement of l-Arginine by d-Arginine and the addition of PEG chains. Firstly, eight OM19r series of antibacterial peptides were obtained by designing D-Arg. Then, a polyethylene glycol-modified product mPEG-butyrALD-OM19r-8 (mPEG-OM19r-8) was isolated and purified by reverse-phase high-performance liquid chromatography (RT-HPLC). The enzyme stability test showed that the resistance of antibacterial peptide OM19r-8 to protease degradation increased by 4-32-fold. Moreover, the Time-kill studies showed that the germicidal kinetics curves of mPEG-OM19r-8 and OM19r-8 to Escherichia coli had a similar trend, thus suggesting that PEG modification has an acceptable effect on the activity of the original peptide. Furthermore, the elimination of half-life (28.09 ± 2.81min) of mPEG-OM19r-8, and the area under the drug concentration-time curve (2686.48 ± 651.36min∗ug/ml) was significantly prolonged. The current study demonstrates an example that optimizes the AMP by utilizing L-to-D amino acid replacement and including PEG chains. These results provide useful data for the clinical application of the mPEG-OM19r-8.
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http://dx.doi.org/10.1016/j.xphs.2020.10.048DOI Listing
March 2021

Developing an optical design pipeline for correcting lens aberrations and vignetting in light field cameras.

Opt Express 2020 Oct;28(22):33632-33643

Light field cameras have been employed in myriad applications thanks to their 3D imaging capability. By placing a microlens array in front of a conventional camera, one can measure both the spatial and angular information of incoming light rays and reconstruct a depth map. The unique optical architecture of light field cameras poses new challenges on controlling aberrations and vignetting in lens design process. The results of our study show that field curvature can be numerically corrected for by digital refocusing, and vignetting must be minimized because it reduces the depth reconstruction accuracy. To address this unmet need, we herein present an optical design pipeline for light field cameras and demonstrated its implementation in a light field endoscope.
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http://dx.doi.org/10.1364/OE.399735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679190PMC
October 2020

Exploring spatio-temporal patterns in mental health related emergency department use from children and adolescents.

Spat Spatiotemporal Epidemiol 2020 08 20;34:100358. Epub 2020 Jun 20.

Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

To understand the spatio-temporal patterns and associated risk factors with the frequency, we analyze records of mental health related emergency department (MHED) visits from youth. The data are extracted for the period 2002--2011 from the population-based, provincial health administrative data systems of Alberta, Canada. Guided by a descriptive analysis, we conduct generalized linear regression analyses of the counts of MHED visits from various health areas. Seasonal effects are examined via three different types of functions, including trigonometric functions. We specify the temporal correlation using an autoregressive model of order 1 and formulate the spatial correlation by a random effects model. Our analysis reveals a strong seasonal pattern and indicates that the MHED visit counts are significantly associated with age, gender, and a proxy for socio-economic status. The final statistical model may be used to forecast future MHED use and identify regions and groups at a higher risk to the MHEDs.
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http://dx.doi.org/10.1016/j.sste.2020.100358DOI Listing
August 2020

Acupuncture for Hormone Therapy-Related Side Effects in Breast Cancer Patients: A GRADE-Assessed Systematic Review and Updated Meta-Analysis.

Integr Cancer Ther 2020 Jan-Dec;19:1534735420940394

Lanzhou University, Gansu, China.

To determine the efficacy of acupuncture on the management of hormone therapy-related side effects in breast cancer patients. Randomized controlled trials of acupuncture versus a control or placebo in breast cancer patients that examined reductions in therapy-related side effects were retrieved from PubMed, EMBASE, Web of Science, and the Cochrane Library through April 2020. Data on patient symptoms (hot flashes, fatigue, pain, stiffness, and gastrointestinal symptoms), physical capacity, cytokines, and general psychosomatic well-being were analyzed. We evaluated and analyzed the quality of all included studies with the 5.2 Cochrane Handbook standards using Stata software (version 10.0) and Revman software (version 5.2), respectively. We assessed the risk of bias using the Cochrane Risk of Bias tool and evaluated the quality of evidence using the GRADE (Grading of Recommendations, Assessment, Development, and Evaluations) approach. The pooled results suggested that acupuncture led to moderate improvements in hot flashes, fatigue, and stiffness. No significant differences were observed in pain, gastrointestinal symptoms, Kupperman index scores, Overall quality of life, tumor necrosis factor levels, and interleukin levels. Evidence for outcome indicators of symptom management were downgraded by the GRADE system for inconsistency, indirectness, and imprecision in the included RCTs. Nonetheless, acupuncture is a moderately appropriate alternative therapy for hormone therapy-related side effects in breast cancer patients. However, it still lacks large-sample, multicenter, prospective RCTs. Future research should focus on standardizing comparison groups and treatment methods, be at least single-blinded, assess biologic mechanisms, have adequate statistical power, and involve multiple acupuncturists.
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http://dx.doi.org/10.1177/1534735420940394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388099PMC
August 2021

Development of a fast calibration method for image mapping spectrometry.

Appl Opt 2020 Jul;59(20):6062-6069

An image mapping spectrometer (IMS) is a snapshot hyperspectral imager that simultaneously captures both the spatial (, ) and spectral () information of incoming light. The IMS maps a three-dimensional (3D) datacube (, , ) to a two-dimensional (2D) detector array (, ) for parallel measurement. To reconstruct the original 3D datacube, one must construct a lookup table that connects voxels in the datacube and pixels in the raw image. Previous calibration methods suffer from either low speed or poor image quality. We herein present a slit-scan calibration method that can significantly reduce the calibration time while maintaining high accuracy. Moreover, we quantitatively analyzed the major artifact in the IMS, the striped image, and developed three numerical methods to correct for it.
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http://dx.doi.org/10.1364/AO.395988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418183PMC
July 2020

The mediating role of perceived social support between anxiety symptoms and life satisfaction in pregnant women: a cross-sectional study.

Health Qual Life Outcomes 2020 Jul 10;18(1):223. Epub 2020 Jul 10.

Department of Social Medicine, School of Public Health, China Medical University, Shenyang, 110122, China.

Background: Pregnancy can be stressful for women and families, so the life satisfaction of pregnant women may face significant challenges. This study aimed to identify the relationship among anxiety symptoms, perceived social support and life satisfaction, and to further explore whether perceived social support can play a mediating role.

Methods: This cross-sectional study was conducted from June to September in Shenyang City, China in 2019. 290 effective questionnaires were collected. The Satisfaction with Life Scale (SWLS), the Zung's Self-Rating Anxiety Scale (SAS), the Multi-Dimensional Scale of Perceived Social Support (MSPSS) as well as demographic variables were included in each questionnaire. Hierarchical multiple regression was conducted to explore the mediating role of perceived social support in the relationship between anxiety symptoms and life satisfaction. Then the mediation model was examined by the PROCESS macro for SPSS.

Results: After adjusting control variables, anxiety symptoms were negatively associated with life satisfaction and explained 14.7% of the variance. Higher level of perceived social support was related to higher level of life satisfaction, explaining 21.0% of the variance. Perceived social support partly mediated the relationship between anxiety symptoms and life satisfaction for pregnant women.

Conclusions: Perceived social support played a mediating role between anxiety symptoms and life satisfaction among pregnant women. Strategies and measures to improve perceived social support may be expected to buffer the impact of anxiety symptoms on pregnant women's life satisfaction.
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http://dx.doi.org/10.1186/s12955-020-01479-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348126PMC
July 2020

PDK1 Regulates the Maintenance of Cell Body and the Development of Dendrites of Purkinje Cells by pS6 and PKCγ.

J Neurosci 2020 07 2;40(29):5531-5548. Epub 2020 Jun 2.

Department of Neurobiology, Key Laboratory of Human Functional Genomics of Jiangsu, Nanjing Medical University, Nanjing 211166, Jiangsu, People's Republic of China

3-Phosphoinositide-dependent protein kinase-1 (PDK1) plays a critical role in the development of mammalian brain. Here, we investigated the role of PDK1 in Purkinje cells (PCs) by generating the PDK1-conditional knock-out mice (cKO) through crossing or mice with mice. The male mice were used in the behavioral testing, and the other experiments were performed on mice of both sexes. These PDK1-cKO mice displayed decreased cerebellar size and impaired motor balance and coordination. By the electrophysiological recording, we observed the reduced spontaneous firing of PCs from the cerebellar slices of the PDK1-cKO mice. Moreover, the cell body size of PCs in the PDK1-cKO mice was time dependently reduced compared with that in the control mice. And the morphologic complexity of PCs was also decreased after PDK1 deletion. These effects may have contributed to the reduction of the rpS6 (reduced ribosomal protein S6) phosphorylation and the PKCγ expression in PDK1-cKO mice since the upregulation of pS6 by treatment of 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-1, the agonist of mTOR1, partly rescued the reduction in the cell body size of the PCs, and the delivery of recombinant adeno-associated virus-PKCγ through cerebellar injection rescued the reduced complexity of the dendritic arbor in PDK1-cKO mice. Together, our data suggest that PDK1, by regulating rpS6 phosphorylation and PKCγ expression, controls the cell body maintenance and the dendritic development in PCs and is critical for cerebellar motor coordination. Here, we show the role of 3-phosphoinositide-dependent protein kinase-1 (PDK1) in Purkinje cells (PCs). The ablation of PDK1 in PCs resulted in a reduction of cell body size, and dendritic complexity and abnormal spontaneous firing, which attributes to the motor defects in PDK1-conditional knock-out (cKO) mice. Moreover, the ribosomal protein S6 (rpS6) phosphorylation and the expression of PKCγ are downregulated after the ablation of PDK1. Additionally, upregulation of rpS6 phosphorylation by3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-1 partly rescued the reduction in cell body size of PCs, and the overexpression of PKCγ in PDK1-KO PCs rescued the reduction in the dendritic complexity. These findings indicate that PDK1 contributes to the maintenance of the cell body and the dendritic development of PCs by regulating rpS6 phosphorylation and PKCγ expression.
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http://dx.doi.org/10.1523/JNEUROSCI.2496-19.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363466PMC
July 2020

The Mediating Role of Psychological Capital in the Relationship Between Occupational Stress and Fatigue: A Cross-Sectional Study Among 1,104 Chinese Physicians.

Front Public Health 2020 28;8:12. Epub 2020 Feb 28.

Department of Social Medicine, School of Public Health, China Medical University, Shenyang, China.

This study aimed to explore the association of occupational stress with fatigue and to examine the mediating role of psychological capital (PsyCap) among Chinese physicians. A cross-sectional study was conducted in Liaoning province, China, in 2018. Using a multistage stratified sampling method, a total of 1,500 physicians participated and 1,104 (73.6%) physicians responded effectively. The study used a self-administered questionnaire consisting of the 14-item Fatigue Scale (FS-14), the Effort-reward Imbalance questionnaire (ERI), the Psychological Capital Questionnaire (PCQ) and items about demographic and working characteristics. Hierarchical multiple regression analyses were performed to explore the association of occupational stress, PsyCap, and fatigue among physicians. Asymptotic and resampling strategies were used to examine the mediating effect. The incidence of fatigue among Chinese physicians was 83.70%. The average level of fatigue was 7.96 ± 3.95 (mean ± SD). Occupational stress and PsyCap were significantly associated with fatigue. PsyCap significantly mediated the association of ERR ( × = 0.106, bias-corrected and accelerated 95% confidence interval [BCa 95% CI]: 0.078, 0.138) and overcommitment ( × = 0.068, BCa 95% CI: 0.044, 0.092) with fatigue. Two important components of PsyCap, self-efficacy and resilience, play more important roles in the mediating effect. The level of fatigue among Chinese physicians was high, which should be taken seriously by management. PsyCap could mediate the association between occupational stress and fatigue. The intervention strategies and measures to relieve fatigue could be focused on physicians' positive PsyCap improvement.
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http://dx.doi.org/10.3389/fpubh.2020.00012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058796PMC
May 2021

Oral administration of the iron chelator deferiprone protects against loss of retinal ganglion cells in a mouse model of glaucoma.

Exp Eye Res 2020 04 8;193:107961. Epub 2020 Feb 8.

Department of Ophthalmology, Scheie Eye Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, USA.

Glaucoma is a progressive neurodegenerative process affecting the retinal ganglion cells (RGCs) and the optic nerve. Oxidative stress has been implicated in glaucoma pathogenesis, and iron is a potent generator of oxidative stress. The oral iron chelator deferiprone (DFP) is protective against retinal degenerations associated with oxidative stress. To test whether DFP could be protective in glaucoma, we used microbead injections to induce elevated intraocular pressure (IOP) in a cohort of 3-month old C57BL/6J mice. One eye of each animal was injected with magnetic microbeads resulting in ocular hypertension for >7 weeks while the fellow eye was injected with saline and served as a normotensive internal control. While half of the cohort received oral DFP (1 mg/ml in the drinking water), the other half did not and served as controls. After 8 weeks, Brn3a immunolabeling of flat-mounted retinas was used for manual RGC quantification. Axon counts were obtained from thin sections of optic nerves using the AxonJ plugin for ImageJ. DFP administration was protective against RGC and optic nerve loss in the setting of elevated IOP. These results suggest that iron chelation by DFP may provide glaucoma neuroprotection.
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http://dx.doi.org/10.1016/j.exer.2020.107961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584350PMC
April 2020

Evaluation of a multimedia marketing campaign to engage African American patients in glaucoma screening.

Prev Med Rep 2020 Mar 23;17:101057. Epub 2020 Jan 23.

Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, USA.

Our objective was to determine which messaging approaches from a marketing campaign were most effective in recruiting African American individuals to a glaucoma screening and research study. We conducted a multimedia marketing campaign in Philadelphia from 01/31/2018 to 06/30/2018. Messaging approaches included radio advertisements and interviews (conducted in partnership with a local radio station with a large African American listener base), print materials, event tables, and online postings. Participants received free glaucoma screenings and the opportunity to enroll in our glaucoma genetics study. These screenings allowed individuals with glaucoma to receive a full examination and treatment plan with a glaucoma specialist, as well as to contribute to future efforts to identify genetic variants underlying this disease. We compared inquiry, enrollment, and cost yield for each messaging approach. Our campaign resulted in 154 unique inquiries, with 98 patients receiving glaucoma screenings (64%) and 60 patients enrolling in our study (39%). Commercials on WURD radio yielded the highest number of inquiries (62%) and enrollments (62%), but at relatively high cost ($814/enrolled patient). The most inexpensive approach that yielded more than five enrollments was postcards ($429/enrolled patient). Our campaign suggests that high-frequency commercials and postcards distributed at targeted healthcare locations are particularly effective and affordable options for connecting with the African American community. Our findings can help to inform recruitment efforts for other understudied diseases in minority populations.
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http://dx.doi.org/10.1016/j.pmedr.2020.101057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997297PMC
March 2020

Snapshot hyperspectral light field imaging using image mapping spectrometry.

Opt Lett 2020 Feb;45(3):772-775

In this Letter, we present a snapshot hyperspectral light field imaging system using a single camera. By integrating an unfocused light field camera with a snapshot hyperspectral imager, the image mapping spectrometer, we captured a five-dimensional (5D) ($x,y,u,v,\lambda $x,y,u,v,λ) ($x,y,$x,y, spatial coordinates; $u,v,$u,v, emittance angles; $\lambda ,$λ, wavelength) datacube in a single camera exposure. The corresponding volumetric image ($x,y,z$x,y,z) at each wavelength is then computed through a scale-depth space transform. We demonstrated the snapshot advantage of our system by imaging the spectral-volumetric scenes in real time.
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http://dx.doi.org/10.1364/OL.382088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472785PMC
February 2020

Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO/graphene oxide nanozyme as a peroxidase mimic.

Mikrochim Acta 2020 01 8;187(2):110. Epub 2020 Jan 8.

CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS center for Excellence in Nanoscience, National Center for Nanoscience and Technology, UCAS, Beijing, 100190, People's Republic of China.

The authors describe a peroxidase-mimicking nanozyme composed of IrO and graphene oxide (GO). It was synthesized from monodisperse IrO nanoparticles with an average diameter of 1.7 ± 0.3 nm that were prepared by pulsed laser ablation in ethanol. The nanoparticles were then placed on polyallylamine-modified GO nanosheets through electrostatic interaction. The peroxidase-like activity of the resulting nanocomposites was evaluated by catalytic oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of HO. Kinetic results demonstrated that the catalytic behavior of the nanocomposites follows Michaelis-Menten kinetics. Experiments performed with terephthalic acid and cytochrome C confirmed that the peroxidase-like activity originated from the electron transfer mechanism rather than from generation of hydroxy radicals. The peroxidase-like activity is inhibited in the presence of ascorbic acid (AA). Based on this property, a colorimetric assay was developed for the determination of AA by exploiting the peroxidase-like activity of IrO/GO nanocomposites. The linear relationship between absorbance at 652 nm and the concentration of AA was acquired. The limit of detection for AA is 324 nM. Further applications of the method for AA detection in real samples were also successfully demonstrated. Graphical abstractSchematic of the preparation of polyallylamine (PAH)-stabilized IrO/GO nanocomposites and the colorimetric detection of AA based on the peroxidase-like activity of IrO/GO nanocomposites.
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http://dx.doi.org/10.1007/s00604-019-3897-4DOI Listing
January 2020

Bacterial removal performance and community changes during advanced treatment process: A case study at a full-scale water reclamation plant.

Sci Total Environ 2020 Feb 27;705:135811. Epub 2019 Nov 27.

Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, PR China. Electronic address:

Advanced treatment is of great significance to water reclamation and reuse, which can improve water quality, control microbial risks and guarantee the safety of water reuse. This study evaluates the microbial quantity and bacterial community dynamics during advanced wastewater treatment and reuse processes (i.e. denitrification biofilter (DNBF), ultrafiltration (UF), ozonation, ultraviolet (UV) disinfection) at a large-scale water reclamation plant. It is found that different treatment processes had significant influence on the cultivability of total bacteria and the log reduction values of fecal coliform at DNBF, UF, ozonation and UV are calculated as 0.38, 2.46, 0.38 and 1.63 respectively. Moreover, the bacterial diversity in the treatment process showed apparent spatial differences, among which the effluent from ozonation process had the lowest bacterial diversity. Sequencing analysis indicated the existence of pathogenic bacterium such as Arcobacter, Bacteroides and Pseudomonas in the secondary effluent. Notably, Pseudomonas remained the most dominant species (relative abundance 41.9% in UV effluent) in reclaimed water after advanced treatment processes, which calls for high attention to sustainable water reuse. In order to inhibit bacterial regrowth in the storage tank, chlorine disinfection is recommended to improve the continuous disinfection capability of the system.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135811DOI Listing
February 2020

Berberine reduces inflammation of human dental pulp fibroblast via miR-21/KBTBD7 axis.

Arch Oral Biol 2020 Feb 4;110:104630. Epub 2019 Dec 4.

Department of Periodontology, Qingdao Stomatological Hospital, Qingdao, Shandong 266001, PR China. Electronic address:

Objective: This study is to investigate the effect and underlying mechanisms of berberine (BBR) on the proliferation and inflammatory levels of lipopolysaccharide induced human dental pulp fibroblast (LPS-HDPF).

Methods: Different concentrations of LPS were used to induce inflammatory response of HDPF. Cell proliferation was observed in BBR treated HDPF after transfection of miR-21 mimic, miR-21 inhibitor or sh-kBTBD7 plasmids and their negative controls. ELISA was employed to detect the expressions of inflammation related cytokines. Real-time quantitative RT-PCR was applied to estimate the expressions of miR-21 and KBTBD7. KBTBD7 expression was detected by Immunocytochemistry. Western blot analysis showed the protein levels of KBTBD7, Phospho-IKKα/β, IKKβ, Phospho-NF-κB p65, NF-κB p65, and IκBα. Luciferase reporter gene assay was used to determine the interaction between miR-21 and KBTBD7.

Results: LPS could promote inflammatory response in HDPF. Down-regulated miR-21 and up-regulated KBTBD7 were found in LPS-HDPF. BBR (25 uM) treatment in LPS-HDPF could ameliorate cell inflammatory response, presented by reduced expressions of IL-1β, IL-6 and TNF-α, as well as enhanced cell proliferation and miR-21 expression. Moreover, miR-21 negatively targets KBTBD7. Over-expression of miR-21 or silencing of KBTBD7 could enhance the protective role of BBR on LPS-HDPF by inhibiting inflammatory response and promoting cell proliferation. Transfection of miR-21 overexpression or KBTBD7 silencing in BBR treated LPS-HDPF could inhibit activation of NF-κB signal pathway.

Conclusion: Evidence in this study suggested that BBR mediates LPS induced inflammation in HDPF via miR-21/KBTBD7 axis to regulate NF-κB signal pathway, which may provide theoretical basis for BBR in prevention of pulpitis.
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http://dx.doi.org/10.1016/j.archoralbio.2019.104630DOI Listing
February 2020

Patient-reported outcomes measures and patient preferences for minimally invasive glaucoma surgical devices.

Eye (Lond) 2020 01 26;34(1):205-210. Epub 2019 Nov 26.

Stanford University School of Medicine, Stanford, CA, USA.

Background: Many therapeutic options are available to glaucoma patients. One recent therapeutic option is minimally invasive glaucoma surgical (MIGS) devices. It is unclear how patients view different treatments and which patient-reported outcomes would be most relevant in patients with mild to moderate glaucoma. We developed a questionnaire for patients eligible for MIGS devices and a patient preference study to examine the value patients place on certain outcomes associated with glaucoma and its therapies.

Objectives: To summarize the progress to date.

Methods: Questionnaire development: We drafted the questionnaire items based on input from one physician and four patient focus groups, and a review of the literature. We tested item clarity with six cognitive interviews. These items were further refined. Patient preference study: We identified important benefit and risk outcomes qualitatively using semi-structured, one-on-one interviews with patients who were eligible for MIGS devices. We then prioritized these outcomes quantitatively using best-worst scaling methods.

Results: Questionnaire testing: Three concepts were deemed relevant for the questionnaire: functional limitations, symptoms, and psychosocial factors. We will evaluate the reliability and validity of the 52-item draft questionnaire in an upcoming field test. Patient preference study: We identified 13 outcomes that participants perceived as important. Outcomes with the largest relative importance weights were "adequate IOP control" and "drive a car during the day."

Conclusions: Patients have the potential to steer clinical research towards outcomes that are important to them. Incorporating patients' perspectives into the MIGS device development and evaluation process may expedite innovation and availability of these devices.
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http://dx.doi.org/10.1038/s41433-019-0676-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002517PMC
January 2020

The Mechanism Underlying Change in the Sex Gap in Life Expectancy at Birth: An Extended Decomposition.

Demography 2019 12;56(6):2307-2321

School of Demography, Australian National University, Canberra, Australia.

The relationship between differential mortality rates and differences in life expectancy is well understood, but how changing differential rates translate into changing differences in life expectancy has not been fully explained. To elucidate the mechanism involved, this study extends existing decomposition methods. The extended method decomposes change in the sex gap in life expectancy at birth into three components capturing the effects of the sex difference in mortality improvement (ρ-effect), life table deaths density by age (f-effect), and remaining life expectancy by age (e-effect). These three effects oppose and augment each other, depending on relative change in sex-differential mortality rates. The new method is applied to period data for 35 countries and cohort data for 25 countries. The results demonstrate how the mechanism, involving the three effects, operates to determine change in the sex difference in life expectancy. We observe the pivotal importance of the f-effect, which is predominantly negative because of lower female mortality, in favoring narrowing rather than widening of the sex gap, in shifting the overall effect to younger ages, and in exaggerating fluctuations due to crisis mortality. The new decomposition provides a more detailed basis for substantive analyses examining change in differences in life expectancy.
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http://dx.doi.org/10.1007/s13524-019-00832-zDOI Listing
December 2019

Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression.

Neurosci Bull 2020 Apr 23;36(4):359-371. Epub 2019 Oct 23.

Department of Medical Genetics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.

Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8 (HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial-mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome.
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http://dx.doi.org/10.1007/s12264-019-00436-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142204PMC
April 2020

Rh-Catalyzed Cycloisomerization of 1,7-Ene-Dienes to Synthesize -Divinylpiperidines: A Formal Intramolecular Addition Reaction of Allylic C-H Bond into Dienes.

Org Lett 2019 Oct 16;21(19):7692-7696. Epub 2019 Sep 16.

Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry , Peking University , Beijing 100871 , China.

An originally designed Rh-catalyzed [4 + 2] cycloaddition reaction of nitrogen-tethered 1,7-ene-dienes turned out to be a cycloisomerization reaction, which involves allylic C-H activation/alkene insertion into Rh-H bond/reductive elimination processes. Deuterium labeling experiments gave support to the proposed mechanism. This unexpected cycloisomerization reaction provides an efficient way to synthesize -divinylpiperidines from easily accessed linear 1,7-ene-dienes.
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http://dx.doi.org/10.1021/acs.orglett.9b02319DOI Listing
October 2019

An anti-forensic scheme on computer graphic images and natural images using generative adversarial networks.

Math Biosci Eng 2019 05;16(5):4923-4935

School of Engineering and Applied Science, The George Washington University, The Cloyd Heck Marvin Center, 800 21st Street, NW-Suite 505, Washington, DC 20052, US.

Computer graphic images (CGI) can be manufactured very similar to natural images (NI) by state-of-the-art algorithms in computer graphic filed. Thus, there are various identification algorithms proposed to detect CGI. However, the manipulation is complicated and difficult for an ultimate CGI against the forensic algorithms. Further, the forensics on CGI and NI made achievements in the different aspects with the encouragement of deep learning. Though the generated CGI can achieve high quality automatically by generative adversarial networks (GAN), CGI generation based on GAN is difficult to ensure that it cannot be detected by forensics. In this paper, we propose a brief and effective architecture based on GAN for preventing the generated images being detected under the forensics on CGI and NI. The adapted characteristics will make the CGI generated by GAN fools the detector and keep the end-to-end generation mode of GAN.
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http://dx.doi.org/10.3934/mbe.2019248DOI Listing
May 2019

APC/Cdh1 targets PECAM-1 for ubiquitination and degradation in endothelial cells.

J Cell Physiol 2020 03 5;235(3):2521-2531. Epub 2019 Sep 5.

Advanced Institute for Medical Sciences, Dalian Medical University, Dalian, China.

Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a member of the immunoglobulin superfamily and is expressed by hematopoietic and endothelial cells (ECs). Recent studies have shown that PECAM-1 plays a crucial role in promoting the development of the EC inflammatory response in the context of disturbed flow. However, the mechanistic pathways that control PECAM-1 protein stability remain largely unclear. Here, we identified PECAM-1 as a novel substrate of the APC/Cdh1 E3 ubiquitin ligase. Specifically, lentivirus-mediated Cdh1 depletion stabilized PECAM-1 in ECs. Conversely, overexpression of Cdh1 destabilized PECAM-1. The proteasome inhibitor MG132 blocked Cdh1-mediated PECAM-1 degradation. In addition, Cdh1 promoted K48-linked polyubiquitination of PECAM-1 in a destruction box-dependent manner. Furthermore, we demonstrated that compared with pulsatile shear stress (PS), oscillatory shear stress decreased the expression of Cdh1 and the ubiquitination of PECAM-1, therefore stabilizing PECAM-1 to promote inflammation in ECs. Hence, our study revealed a novel mechanism by which fluid flow patterns regulate EC homeostasis via Cdh1-dependent ubiquitination and subsequent degradation of PECAM-1.
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http://dx.doi.org/10.1002/jcp.29156DOI Listing
March 2020

Stachydrine Mediates Rapid Vascular Relaxation: Activation of Endothelial Nitric Oxide Synthase Involving AMP-Activated Protein Kinase and Akt Phosphorylation in Vascular Endothelial Cells.

J Agric Food Chem 2019 Sep 21;67(35):9805-9811. Epub 2019 Aug 21.

Cardiovascular Research Center, School of Basic Medical Sciences , Xi'an Jiaotong University , Xi'an 710061 , China.

Stachydrine (STA) is a constituent of citrus fruits and Sweet. In the present study, we established that STA caused acute endothelium-dependent relaxation. The vascular action of STA was mediated by nitric oxide (NO) via cyclic guanosine monophosphate. Mechanistically, STA activated AMP-activated protein kinase (AMPK), protein kinase B/Akt, and endothelial NO synthase (eNOS) in vascular endothelial cells (ECs). AMPK inhibition by compound C blocked STA-induced Akt/eNOS phosphorylation, suggesting that AMPK is the upstream of Akt and eNOS. Inhibition of Akt by MK2206 blocked STA-stimulated eNOS phosphorylation without altering AMPK phosphorylation. Furthermore, we showed that STA activated AMPK via the induction of liver kinase B1 phosphorylation. These results indicated that STA can induce eNOS phosphorylation and vasorelaxation by regulating the interplay between AMPK and Akt pathways in ECs. These findings further highlighted the potential of STA as a nutritional factor in the beneficial effects of fruit intake in preventing the endothelial dysfunction-related metabolic cardiovascular diseases.
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http://dx.doi.org/10.1021/acs.jafc.9b03501DOI Listing
September 2019

Primary Open-Angle African American Glaucoma Genetics (POAAGG) Study: gender and risk of POAG in African Americans.

PLoS One 2019 1;14(8):e0218804. Epub 2019 Aug 1.

Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, United States of America.

The purpose of this study was to investigate the association between gender and primary open-angle glaucoma (POAG) among African Americans and to assess demographic, systemic, and behavioral factors that may contribute to differences between genders. The Primary Open-Angle African American Glaucoma Genetics (POAAGG) study had a case-control design and included African Americans 35 years and older, recruited from the greater Philadelphia, Pennsylvania. Diagnosis of POAG was based on evidence of both glaucomatous optic nerve damage and characteristic visual field loss. Demographic and behavioral information, history of systemic diseases and anthropometric measurements were obtained at study enrollment. Gender differences in risk of POAG were examined using multivariate logistic regression. A total of 2,290 POAG cases and 2,538 controls were included in the study. The percentage of men among cases was higher than among controls (38.6% vs 30.3%, P<0.001). The subjects' mean age at enrollment was significantly higher for cases compared to controls (70.2±11.3 vs. 61.6±11.8 years, P<0.003). Cases had lower rates of diabetes (40% vs. 46%, P<0.001), higher rates of systemic hypertension (80% vs. 72%, P<0.001), and lower body mass index (BMI) (29.7±6.7 vs. 31.9±7.4, P<0.001) than controls. In the final multivariable model, male gender was significantly associated with POAG risk (OR, 1.64; 95% CI, 1.44-1.87; P<0.001), after adjusting for age, systemic hypertension, diabetes, and BMI. Within the POAAGG study, men were at higher risk of having POAG than women. Pending genetic results from this study will be used to better understand the underlying genetic variations that may account for these differences.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0218804PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675103PMC
March 2020

An Efficient Strategy Based on Liquid-Liquid Extraction With Acid Condition and HSCCC for Rapid Enrichment and Preparative Separation of Three Caffeoylquinic Acid Isomers From Mulberry Leaves.

J Chromatogr Sci 2019 Aug;57(8):738-744

The College of Forestry, Beijing Forestry University, Beijing, People's Republic of China.

Morus alba L. is a medicinal plant that contains a high amount of caffeoylquinic acids such as 3-caffeoylquinic acid (3-CQA), 5-caffeoylquinic acid (5-CQA) and 4-caffeoylquinic acid (4-CQA). This study aimed to establish a fast and efficient method for separating caffeoylquinic acids from mulberry leaves by using high-speed countercurrent chromatography coupled with macroporous resin. D101 resin showed better adsorption and desorption capacity for three caffeoylquinic acids among six macroporous resin adsorbents. The contents of 3-CQA, 5-CQA and 4-CQA reached for 4.77%, 18.95% and 9.84% through one cycle of D101 resin, which were 3.13-fold, 4.57-fold and 4.78-fold more than those in crude extracts, respectively. With a two-phase solvent system of ethyl acetate-water (1:1, V/V), >93% purity of target compounds were obtained in one cycle during 150 min with the recovery yields of 80.59%, 99.56% and 94.21% for 3-CQA, 5-CQA and 4-CQA, respectively. The structural identification of target compounds was carried out by ESI-MS, 1H NMR and 13C NMR spectra. The present result represented an easy and efficient separation strategy for the utilization of mulberry resource.
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http://dx.doi.org/10.1093/chromsci/bmz050DOI Listing
August 2019

MARVELD1 attenuates arsenic trioxide-induced apoptosis in liver cancer cells by inhibiting reactive oxygen species production.

Ann Transl Med 2019 May;7(9):200

Department of Medical Genetics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

Background: Arsenic trioxide (AsO) is widely used for the treatment of acute promyelocytic leukemia (APL), and more recently, has also been applied to solid tumors. However, there are a fraction of patients with solid tumors, such as liver cancer, who respond to AsO treatment poorly. The underlying mechanisms for this remain unclear.

Methods: We determined the suitable concentration of drugs by IC50. Cell Counting Kit-8 (CCK-8) and flow cytometry were used to analyze the apoptosis. Morphological changes of the cells were observed by laser scanning confocal microscopy. Furthermore, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected by flow cytometry. Quantitative polymerase chain reaction (qPCR) and Western blot tests were conducted to detect the mRNA and protein levels in different groups. Finally, a xenograft tumor assay and histopathological analysis were performed to evaluate the MARVELD1 function in cell proliferation and apoptosis.

Results: Here, we show that MARVELD1 enhances the therapeutic effects of epirubicin, while inducing the strong resistance of liver cancer cells to AsO treatment. We further demonstrate that the AsO-induced apoptosis was inhibited by MARVELD1 overexpression (24 h Vector MARVELD1 =30.58% 17.41%, P<0.01; 48 h Vector MARVELD1 =46.50% 21.02%, P<0.01), possibly through inhibiting ROS production by enhancing TRXR1 expression. , we found a significantly increased size (Vector MARVELD1 =203.90±21.92 675.70±37.84 mm, P<0.001) and weight (Vector MARVELD1 =0.19±0.02 0.58±0.05 g, P<0.001) of tumors with high expression of MARVELD1 after AsO treatment. Consistently, a higher expression of MARVELD1 predicted a poor prognosis for liver cancer patients.

Conclusions: Our data identified a unique role of MARVELD1 in AsO-induced apoptosis and AsO cancer therapy resistance.
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http://dx.doi.org/10.21037/atm.2019.04.38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545301PMC
May 2019
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