Publications by authors named "Purav Badani"

7 Publications

  • Page 1 of 1

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas Advancement in Molecular Diagnostics and Signal Readout Approaches.

J Mol Diagn 2021 11 25;23(11):1433-1442. Epub 2021 Aug 25.

Department of Electrochemical and Molecular Biology, Canary Global Inc., Burlington, Ontario, Canada.

Discovery of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has greatly improved the gene editing technology. Their applications in the area of diagnostic innovation are gaining much attention. The key characteristics of CRISPR/Cas system that allowed its extensive exploitation in the detection platform are their programmable and highly selective target recognition scheme. Herein, this review presents the major three Cas effectors (Cas9, Cas12, and Cas13) and their significance in various detection assays. The CRISPR/Cas detection strategies, based on their target hybridization, cleavage activity, sensor capabilities, and signal readout methods, are discussed. Some of the recent progressions, challenges, and improvement strategies of CRISPR/Cas technology are highlighted and their biosensing detection platforms toward the development of simple, sensitive, and portable point-of-care diagnostic devices are presented.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmoldx.2021.07.025DOI Listing
November 2021

Design and Development of Axially Chiral Bis(naphthofuran) Luminogens as Fluorescent Probes for Cell Imaging.

Chemistry 2021 Mar 25;27(17):5470-5482. Epub 2021 Feb 25.

Department of Speciality Chemicals Technology, Institute of Chemical Technology (ICT), Mumbai, 400019, India.

Designing chiral AIEgens without aggregation-induced emission (AIE)-active molecules externally tagged to the chiral scaffold remains a long-standing challenge for the scientific community. The inherent aggregation-caused quenching phenomenon associated with the axially chiral (R)-[1,1'-binaphthalene]-2,2'-diol ((R)-BINOL) scaffold, together with its marginal Stokes shift, limits its application as a chiral AIE-active material. Here, in our effort to design chiral luminogens, we have developed a design strategy in which 2-substituted furans, when appropriately fused with the BINOL scaffold, will generate solid-state emissive materials with high thermal and photostability as well as colour-tunable properties. The excellent biocompatibility, together with the high fluorescence quantum yield and large Stokes shift, of one of the luminogens stimulated us to investigate its cell-imaging potential. The luminogen was observed to be well internalised and uniformly dispersed within the cytoplasm of MDA-MB-231 cancer cells, showing high fluorescence intensity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.202004942DOI Listing
March 2021

Cyclic Organoselenide BODIPY-Based Probe: Targeting Superoxide in MCF-7 Cancer Cells.

ACS Omega 2020 Jun 3;5(23):14186-14193. Epub 2020 Jun 3.

Department of Chemistry, University of Mumbai, Santacruz (E), Mumbai 400098, India.

All aerobic cells contain reactive oxygen species (ROSs) in balance with biochemical antioxidants. Oxidative stress is developed when this balance gets disturbed because of excessive production of ROSs or depletion of antioxidants. Here, in this work, we have developed the first cyclic diselenide BODIPY-based (organoselenium-containing) probe for the selective detection of superoxide. The probe demonstrates excellent selective response for superoxide over other ROSs with nine-fold increase in fluorescence intensity. The detection limit was found to be 0.924 μM. The plausible "turn-on" mechanism has been proposed based on the spectroscopic and quantum chemical data. Usefulness of the probe for selective detection of superoxide was confirmed in mammalian breast cancer cell lines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c02074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301547PMC
June 2020

Synthesis of Concave and Vaulted 2-Pyran-Fused BINOLs and Corresponding [5] and [7]-Oxa-helicenoids: Regioselective Cascade-Concerted Route and DFT Studies.

J Org Chem 2020 Jun 4;85(12):7739-7747. Epub 2020 Jun 4.

Department of Chemistry, University of Mumbai, Vidyanagari, Santacruz(East), Mumbai 400098, India.

Synthesis of concave and vaulted 2-pyran-fused BINOLs has been achieved. A regioselective, path-breaking concerted cascade route allows the placement of six-membered heteroaromatic rings at the sterically crowded 7,8 and 7',8' positions of BINOL. DFT studies with relative energetics that support the kinetically controlled reaction pathway are preferred, matching the experimental results. The new BINOLs exhibit smaller dihedral angle than BINOL on the diol part; this structural feature can be an assisting factor for better ligation with metals in the metal-catalyzed reactions. Corresponding symmetric [5] and [7]-oxa-helicenoids have an overlapping, sterically crowded geometry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.0c00363DOI Listing
June 2020

Benzyne-Mediated Nonconcerted Pathway toward Synthesis of Sterically Crowded [5]- and [7]Oxahelicenoids, Stereochemical and Theoretical Studies, and Optical Resolution of Helicenoids.

J Org Chem 2019 01 9;84(2):860-868. Epub 2019 Jan 9.

Department of Chemistry , University of Mumbai , Vidayanagari, Santacruz (East) , Mumbai 400098 , India.

Synthesis of [5]- and [7]oxahelicenoids via Diels-Alder reaction of sterically crowded bichromenes with benzyne is presented. Studies carried out on Diels-Alder addition product establish the unusual preference for a stepwise mechanism over the concerted reaction pathway. This high yielding general synthetic protocol affords unexpected anti cycloadducts [5]- and [7]oxahelicenoids, as confirmed by crystallographic analysis. To rationalize these intriguing antiaddition products, the reaction mechanism was elucidated by means of DFT analysis. Additionally, hydroxy-functionalized [7]oxahelicenoid has been resolved in its optically pure forms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.8b02507DOI Listing
January 2019

Mass spectrometric and charge density studies of organometallic clusters photoionized by gigawatt laser pulses.

Mass Spectrom Rev 2017 03 11;36(2):188-212. Epub 2015 May 11.

Chemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.

Clusters on exposure to nanosecond laser pulses of gigawatt intensity exhibit a variety of photo-chemical processes such as fragmentation, intracluster reaction, ionization, Coulomb explosion, etc. Present article summarizes the experimental results obtained in our laboratory utilizing time-of-flight mass spectrometer which deal with one such aspect of cluster photochemistry related to generation of multiply charged atomic ions upon excessive ionization of cluster constituents (Coulomb explosion) at low intensity laser field (∼10  W/cm ). To understand the mechanism of laser-cluster interaction, laser as well as cluster parameters were varied. Mass spectrometric studies were carried out at different laser wavelength as well as varying the nature of cluster constituents, backup pressure, nozzle diameter, etc. In addition, charge density measurements were also preformed to get information about the total number of ions generated upon laser-cluster interaction as a function of laser wavelength. In case of pure molecular clusters, the charge state of atomic ions as well as charge density was observed to enhance with increasing laser wavelength, signifying efficient coupling of the cluster medium with nanosecond laser pulse at longer wavelength. While in case of clusters doped with species having comparatively lower ionization energy, the efficiency of laser-cluster interaction was less, in contrast to studies carried out using femtosecond lasers. Results obtained in the present work have been rationalized on the basis of proposed three-stage cluster ionization mechanism, that is, multiphoton ionization ignited-inverse Bremsstrahlung heating and electron ionization. © 2015 Wiley Periodicals, Inc. Mass Spec Rev. 36:188-212, 2017.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mas.21469DOI Listing
March 2017

Effect of cluster expansion on photoionization of iron pentacarbonyl doped inert gas clusters under gigawatt intensity laser irradiation.

Rapid Commun Mass Spectrom 2012 Oct;26(19):2204-10

Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.

Rationale: The aim of the investigation was to understand the variation in ionization dynamics of inert gas clusters upon doping with species with lower ionization energy than the inert gas constituent. It was postulated that the use of dopant species having lower ionization energy would lead to facile ionization of doped inert gas clusters, resulting in enhancement of the charge state of atomic ions compared with those obtained for pure inert gas clusters.

Methods: Inert gas clusters (Ar(n), Kr(n) or Xe(n)) doped with iron pentacarbonyl were generated by supersonic expansion and subjected to gigawatt intensity laser pulses (266, 355, 532 and 1064 nm wavelengths) obtained from a nanosecond Nd:YAG laser. The ions generated upon laser-cluster interaction were characterized using a time-of-flight mass spectrometer.

Results: Upon interaction of the laser with the doped inert gas clusters, the charge states of the atomic ions were found to increase with the laser wavelength. However, the highest observed charge states were found to be lower for doped inert gas clusters than for pure inert gas clusters, at all laser wavelengths.

Conclusions: Wavelength-dependent generation of multiply charged atomic ions has been explained based on the three stage model, i.e. multiphoton ionization ignited-inverse bremsstrahlung heating, and electron ionization. This model explains enhancement in the charge state of atomic ions with increasing wavelength based on inverse bremsstrahlung heating of the inner ionized electron, which is a more efficient process at longer wavelengths. Inefficient coupling of laser energy in the case of doped inert gas clusters compared with pure inert gas clusters has been rationalized on the basis of accelerated disintegration of the cluster due to facile initial ionization of dopant molecules having low ionization energy. The results suggest that a longer laser wavelength and a slower rate of cluster expansion facilitate the efficient transfer of optical energy into cluster systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/rcm.6341DOI Listing
October 2012
-->