Publications by authors named "Pu Wang"

654 Publications

Recycling spent ternary lithium-ion batteries for modification of dolomite used in catalytic biomass pyrolysis - A preliminary study by thermogravimetric and pyrolysis-gas chromatography/mass spectrometry analysis.

Bioresour Technol 2021 Oct 1;337:125476. Epub 2021 Jul 1.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CICAEET), School of Environmental Science and Engineering, Nanjing University of Information Science and Technology (NUIST), Nanjing 210044, China. Electronic address:

This paper proposed a novel method for modification of dolomite (Do) using the leaching solution derived from the spent ternary LIBs. During catalytic pyrolysis of biomass, the modified Do showed a good performance on both reducing the activation energy and upgrading the volatile products. The apparent activation energy was decreased from 201 to 180 kJ/mol for the cellulose pyrolysis, and it was decreased from 80 to 75 kJ/mol for the lignin pyrolysis. The cellulose pyrolysis with the modified Do could significantly promote the conversion of anhydrosugars into small-molecule components (e.g., ketones). Meanwhile, the Do modified by transition-metal (e.g., Mn, Co, Ni) oxides had a high catalytic activity in cracking phenols (main tar precursors) to hydrocarbons (e.g., aromatics) during the lignin pyrolysis. The modified Do inhibited the production of phenols (from 50% to 5.8%) and enhanced the production of hydrocarbons (from 0.6% to 30.3%).
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http://dx.doi.org/10.1016/j.biortech.2021.125476DOI Listing
October 2021

Effect of Oral Motor Intervention on Oral Feeding in Preterm Infants: A Systematic Review and Meta-Analysis.

Am J Speech Lang Pathol 2021 Jul 27:1-11. Epub 2021 Jul 27.

Department of Rehabilitation Medicine, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.

Objective This review article aimed to explore the effect of oral motor intervention on oral feeding in preterm infants through a meta-analysis. Method Eligible studies were retrieved from four databases (PubMed, Embase, Cochrane Library, and Web of Science) up to July 2020 and screened based on established selection criteria. Thereafter, relevant data were extracted and heterogeneity tests were conducted to select appropriate effect models according to the chi-square test and statistics. Assessment of risk of bias was performed among the included studies. Finally, a meta-analysis was carried out to evaluate the effect of oral motor intervention in preterm infants according to four clinical indicators: transition time for oral feeding, length of hospital stay, feeding efficiency, and weight gain. Results Eighteen randomized controlled trials with 848 participants were selected to evaluate the effect of oral motor intervention on preterm infants. The meta-analysis results revealed that oral motor intervention could effectively reduce the transition time to full oral feeds and the length of hospital stay as well as increase feeding efficiency and weight gain. Conclusions Oral motor intervention was an effective way to improve oral feeding in preterm infants. It is worthy to be used widely in hospitals to improve the clinical outcomes of preterm infants and reduce the economic burdens of families and society. Future studies should seek to identify detailed intervention processes and intervention durations for clinical application.
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http://dx.doi.org/10.1044/2021_AJSLP-20-00322DOI Listing
July 2021

MEK inhibition by trametinib overcomes chemoresistance in preclinical nasopharyngeal carcinoma models.

Anticancer Drugs 2021 Jul 19. Epub 2021 Jul 19.

Department of Oncology Department of Urologic Surgery Department of Neurology, Xiangyang No. 1 People's Hospital, Affiliated Hospital of Hubei University of Medicine, Xiangyang, Hubei, People's Republic of China.

The development of chemoresistance is the major cause of treatment failure in nasopharyngeal carcinoma (NPC). Although 'paradoxical' activation of extracellular signal-regulated kinase (ERK) has been shown to contribute resistance to anticancer treatment, the role of ERK in NPC chemoresistance has not been yet revealed. In this work, we report that trametinib, a clinically available mitogen-activated protein kinase inhibitor for melanoma treatment, overcomes NPC chemoresistance via suppressing ERK activation induced by chemotherapy. We first showed that trametinib at nanomolar concentrations was active against NPC cells and acted synergistically with cisplatin. Trametinib remarkably decreased phosphorylation of ERK and its downstream effector in NPC cells. We next showed that cisplatin treatment stimulates ERK signaling, and furthermore that this can be abolished by trametinib. We finally generated cisplatin-resistant NPC models and demonstrated that trametinib was effective in inhibiting cisplatin-resistant NPC growth, colony formation and survival via suppressing ERK signaling in vitro and in vivo. Our work demonstrates the potential of trametinib in overcoming chemoresistance in preclinical NPC models and provides evidence of initializing clinical trials of using trametinib for NPC treatment.
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http://dx.doi.org/10.1097/CAD.0000000000001092DOI Listing
July 2021

The effectiveness of eye tracking in the diagnosis of cognitive disorders: A systematic review and meta-analysis.

PLoS One 2021 12;16(7):e0254059. Epub 2021 Jul 12.

Department of Rehabilitation Medicine, The 7th Affiliated Hospital of Sun Yat-Sen University (Shenzhen), Shenzhen, Guangdong, China.

Background: Eye tracking (ET) is a viable marker for the recognition of cognitive disorders. We assessed the accuracy and clinical value of ET for the diagnosis of cognitive disorders in patients.

Methods: We searched the Medline, Embase, Web of Science, Cochrane Library, and Pubmed databases from inception to March 2, 2021, as well as the reference lists of identified primary studies. We included articles written in English that investigated ET for cognitive disorder patients-Mild cognitive impairment (MCI), Alzheimer's disease (AD), Amyotrophic lateral sclerosis (ALS), and dementia. Two independent researchers extracted the data and the characteristics of each study; We calculated pooled sensitivities and specificities. A hierarchical summary of receiver performance characteristics (HSROC) model was used to test the diagnostic accuracy of ET for cognitive impairment (CI).

Findings: 11 studies met the inclusion criteria and were included in qualitative comprehensive analysis. Meta-analysis was performed on 9 trials using Neuropsychological Cognitive Testing (NCT) as the reference standard. The comprehensive sensitivity and specificity of ET for detecting cognitive disorders were 0.75 (95% CI 0.72-0.79) and 0.73 (95% CI 0.70 to 0.76), respectively. The combined positive likelihood ratio (LR+) was 2.74 (95%CI 2.32-3.24) and the negative likelihood ratio (LR-) was 0.27 (95%CI 0.18-0.42).

Conclusions: This review showed that ET technology could be used to detect the decline in CI, clinical use of ET techniques in combination with other tools to assess CI can be encouraged.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254059PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274929PMC
July 2021

An AuS network model for exploring the structural origin, evolution, and two-electron (2e) reduction growth mechanism of Au(SR) clusters.

J Chem Phys 2021 Jun;154(24):244308

Department of Chemistry, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Xiangtan University, Xiangtan, Hunan 411105, China.

An AuS network model was proposed to study the structural origin, evolution, and formation mechanism of the Au(SR) clusters containing quasi-face-centered-cubic (fcc) cores. The Au-S framework structures of 20 quasi-fcc gold clusters had been determined from the AuS network. Based on the AuS network, some new quasi-fcc clusters, such as 8e clusters Au(SR), Au(SR), Au(SR) , Au(SR), Au(SR), and Au(SR), and a class of Au(SR) (n = 1, 2, 3, …) clusters were predicted. Furthermore, by studying the evolution of Au-S frameworks, it was possible to construct molecular-like reaction equations to account for the formation mechanism of quasi-fcc gold clusters, which indicated that the formation of quasi-fcc gold clusters can be understood from the stepwise 2e-reduction cluster growth pathways. The present studies showed that the AuS network model provided a "parental" Au-S network for exploring the structural evolution of the quasi-fcc Au(SR) clusters. Moreover, it was possible to study the formation pathways of the Au(SR) clusters by studying the evolution of their Au-S frameworks.
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http://dx.doi.org/10.1063/5.0047886DOI Listing
June 2021

Assessment of liver fibrosis by transient elastography in young children with chronic hepatitis B virus infection.

Hepatol Int 2021 Jun 9;15(3):602-610. Epub 2021 Jul 9.

Medical School of Chinese PLA, Beijing, China.

Background: This study aimed to compare the diagnostic accuracy of transient elastography (TE) and biopsy for the detection of liver fibrosis in children with chronic hepatitis B (CHB).

Methods: This single-center prospective study included 157 CHB children aged 0-6 years. All patients underwent liver stiffness measurement (LSM) by TE and liver biopsy, separated by an interval of less than 1 week.

Results: The LSM, aspartate aminotransferase-platelet ratio index (APRI), and fibrosis-4 index (FIB-4) were positively correlated with activity grade and fibrosis stage in CHB children. The areas under the receiver operating characteristic curves (AUCs) of LSM for identifying significant (F ≥ 2) and advanced (F ≥ 3) fibrosis were 0.732 and 0.941, respectively. The cut-off values, specificity, and sensitivity for significant fibrosis were 5.6 kPa, 75.7%, and 67.4%, respectively; the corresponding values for advanced fibrosis were 6.9 kPa, 91.5%, and 81.3%, respectively. Compared to LSM, the overall diagnostic performances of APRI and FIB-4 for significant and advanced fibrosis were suboptimal, with low AUCs and sensitivity. Since LSM, platelet, and Log (hepatitis B surface antigen) were independent factors associated with the fibrosis stage (F < 2 and F ≥ 2), they were used to formulate the "LPS" index for the prediction of F ≥ 2. The AUC of LPS (for F ≥ 2) was higher than that of LSM (0.792 vs. 0.732, p < 0.05), and had an improved sensitivity (76.6% vs. 67.4%).

Conclusions: TE is a promising technology for the diagnosis of advanced fibrosis in CHB children aged 0-6 years.
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http://dx.doi.org/10.1007/s12072-021-10194-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286936PMC
June 2021

Efficient Nanocrystal Photovoltaics via Blade Coating Active Layer.

Nanomaterials (Basel) 2021 Jun 9;11(6). Epub 2021 Jun 9.

School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.

CdTe semiconductor nanocrystal (NC) solar cells have attracted much attention in recent year due to their low-cost solution fabrication process. However, there are still few reports about the fabrication of large area NC solar cells under ambient conditions. Aiming to push CdTe NC solar cells one step forward to the industry, this study used a novel blade coating technique to fabricate CdTe NC solar cells with different areas (0.16, 0.3, 0.5 cm) under ambient conditions. By optimizing the deposition parameters of the CdTe NC's active layer, the power conversion efficiency (PCE) of NC solar cells showed a large improvement. Compared to the conventional spin-coated device, a lower post-treatment temperature is required by blade coated NC solar cells. Under the optimal deposition conditions, the NC solar cells with 0.16, 0.3, and 0.5 cm areas exhibited PCEs of 3.58, 2.82, and 1.93%, respectively. More importantly, the NC solar cells fabricated via the blading technique showed high stability where almost no efficiency degradation appeared after keeping the devices under ambient conditions for over 18 days. This is promising for low-cost, roll-by-roll, and large area industrial fabrication.
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http://dx.doi.org/10.3390/nano11061522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226763PMC
June 2021

Effects of UV Irradiation and Storage on the Performance of Inverted Red Quantum-Dot Light-Emitting Diodes.

Nanomaterials (Basel) 2021 Jun 18;11(6). Epub 2021 Jun 18.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou 510640, China.

We report the effects of ultraviolet (UV) irradiation and storage on the performance of ZnO-based inverted quantum-dot light-emitting diodes (QLEDs). The effects of UV irradiation on the electrical properties of ZnO nanoparticles (NPs) were investigated. We demonstrate that the charge balance was enhanced by improving the electron injection. The maximum external quantum efficiency (EQE) and power efficiency (PE) of QLEDs were increased by 26% and 143% after UV irradiation for 15 min. In addition, we investigated the storage stabilities of the inverted QLEDs. During the storage period, the electron current from ZnO gradually decreased, causing a reduction in the device current. However, the QLEDs demonstrated improvements in maximum EQE by 20.7% after two days of storage. Our analysis indicates that the suppression of exciton quenching at the interface of ZnO and quantum dots (QDs) during the storage period could result in the enhancement of EQE. This study provides a comprehension of the generally neglected factors, which could be conducive to the realization of high-efficiency and highly storage-stable practical applications.
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http://dx.doi.org/10.3390/nano11061606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235399PMC
June 2021

Microneme Protein 3 Inhibits Apoptosis of the Chicken Duodenal Epithelial Cell by Targeting the Casitas B-Lineage Lymphoma Protein.

Front Vet Sci 2021 18;8:636809. Epub 2021 May 18.

Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China.

() causes coccidiosis in poultry which persists as economic pain worldwide. Most damage to the intestinal mucosa results from apoptosis of the infected intestinal epithelial cells. The Microneme protein 3 (MIC3) protein is a key virulence factor in some parasites involved in host cell apoptosis inhibition. Here, we studied whether and how MIC3 affects the apoptosis in infected chicken duodenal epithelial cells. Through flow cytometry (FCM), we found that the presence of merozoites and the overexpression of MIC3 significantly decreased apoptosis and the activity of caspase-3 in chicken duodenal epithelial cells at 4, 6, and 8 h post merozoite infection ( < 0.01). Silencing the Casitas B-lineage lymphoma (CBL) protein, a host receptor for MIC3 with shRNA was shown to promote apoptosis in the chicken duodenal epithelial cells. The early apoptotic rate of host cells in the lentiviral-MIC3 group was significantly lower than that in the lentiviral-MIC3 + shRNA CBL group at 4 h after MIC3 expression ( < 0.01), and it was moderately decreased in the lentiviral-MIC3 + shRNA CBL group compared with that in the shRNA CBL group. Our data indicated that MIC3 inhibited early apoptosis of infected chicken duodenal epithelial cells by targeting host receptor-CBL protein. These findings unveiled one of the mechanisms of how intracellular parasites affect the apoptosis of infected host cells, which provided a deeper understanding of their pathogenesis.
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http://dx.doi.org/10.3389/fvets.2021.636809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204691PMC
May 2021

Combined toxicity of nano-TiO and Cd to Scenedesmus obliquus: Effects at different concentration ratios.

J Hazard Mater 2021 Jun 9;418:126354. Epub 2021 Jun 9.

Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

The continuous release of manufactured nanomaterials (MNMs) to environments raised concerns on their combined toxicological risks with co-existing contaminants, since MNMs might severely alter the environmental behavior and fate of the contaminants. In this study, the combined toxicity of nano-sized titanium dioxide (nTiO) and cadmium (Cd) to the green alga Scenedesmus obliquus and the underlying physicochemical mechanisms were investigated for the first time at different concentration ratios of Cd to nTiO to closely mimic the realistic environment scenarios where the concentration ratios of nTiO to other contaminants are constantly changing. Our results suggested that under the co-exposure to different concentration ratios of Cd to nTiO, the co-exposure contaminants exhibited three different combined toxicity modes (antagonistic, partially additive, and synergistic). Specifically, antagonistic combined toxicity was observed under co-exposure to a low concentration ratio of nTiO to Cd as the absorption by nTiO decreased the bioavailability of Cd. However, the partially additive and synergistic combined toxicity occurred when the proportion of nTiO in the co-exposure system was relatively high, which would mechanically and/or oxidatively damage the alga cell structures. Even worse, as a carrier of Cd, nTiO enhanced the amount of Cd entering cells, which significantly enhanced the toxicity of Cd to algae. Overall, we demonstrated that concentration ratios of nTiO to Cd play an important role in determining the combined toxicity mode, which would provide a novel reference to environmental and health risk assessment of co-exposure to conventional pollutants and MNMs.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126354DOI Listing
June 2021

Repetitive Transcranial Magnetic Stimulation at Different Sites for Dysphagia After Stroke: A Randomized, Observer-Blind Clinical Trial.

Front Neurol 2021 26;12:625683. Epub 2021 May 26.

Department of Rehabilitation Medicine, The Seventh Affiliated Hospital Sun Yat-sen University, Shenzhen, China.

The clinical efficacy of repetitive transcranial magnetic stimulation (rTMS) protocols on patients with poststroke dysphagia is still unclear. This trial aimed to explore and analyze the effectiveness of 5 Hz rTMS on the unaffected hemisphere, affected hemisphere, and cerebellum in stroke patients with dysphagia. This observer-blind and randomized controlled trial included a total of 147 patients with stroke. Patients were divided into four treatment groups: the unaffected hemispheric group, the affected hemispheric group, the cerebellum group and the control group. Each group received traditional dysphagia treatment 5 days a week for 2 weeks. All recruited patients except for those in the control group underwent 10 consecutive rTMS sessions for 2 weeks. For the affected hemispheric group and unaffected hemispheric group, 5 Hz rTMS was applied to the affected mylohyoid cortical region or to the unaffected mylohyoid cortical region. For the cerebellum group, 5 Hz rTMS was applied to the mylohyoid cortical representation of the cerebellum (4.3 cm lateral and 2.4 cm below the inion). The Fiberoptic Endoscopic Dysphagia Severity Scale (FEDSS), Penetration/Aspiration Scale (PAS), Gugging Swallowing Screen (GUSS), and Standardized Swallowing Assessment (SSA) were used to evaluate clinical swallowing function before the intervention (baseline), immediately after the intervention and 2 weeks after the intervention. There were significant time and intervention interaction effects on the FEDSS, PAS, SSA, and GUSS scores ( < 0.05). In a direct comparison of the swallowing parameters of the four groups, the changes in FEDSS, PAS, SSA, and GUSS scores showed a significantly greater improvement in the unaffected hemispheric group, the affected hemispheric group and cerebellum group than in the control group ( < 0.05). Whether stimulating the unaffected hemisphere or the affected hemisphere, 5 Hz high-frequency rTMS on mylohyoid cortical tissue might have a positive effect on poststroke patients with dysphagia. In addition, cerebellar rTMS is a safe method that represents a potential treatment for poststroke dysphagia, and more clinical trials are needed to develop this technique further. chictr.org.cn, identifier: ChiCTR2000032255.
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http://dx.doi.org/10.3389/fneur.2021.625683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187758PMC
May 2021

Role of micro-size zero valence iron as particle electrodes in a three-dimensional heterogeneous electro-ozonation process for nitrobenzene degradation.

Chemosphere 2021 Aug 15;276:130264. Epub 2021 Mar 15.

State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, People's Republic of China; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Chongqing University, Chongqing, 400045, People's Republic of China. Electronic address:

A novel water treatment process (designated E-Fe-O process) was constructed by combining electrolysis, micro-size zero valence iron (Fe) and ozone in this study. Compared with other control processes, the combined process demonstrated a remarkable synergy, and it could obtain 90.5% of NB removal within 20 min. As for the mineralization experiment, the TOC removal efficiency for NB within 120 min was higher in the E-Fe-O process, while the energy consumption was lower than the traditional E-O and E-Fe process. Interestingly, hydroxyl radicals (OH) acted as a key role for NB removal, and the concentration of OH in different processes were compared. Further study indicated OH, direct anode oxidation, direct ozonation, and zero valence iron catalysis were all responsible for nitrobenzene removal. Besides, the durability of Fe in the E-Fe-O process was systematically evaluated by reusing Fe 10 times. Notably, the electric field could protect micro-size zero valence iron from passivation for catalytic ozonation after the long-term reaction. Finally, other ozone-refractory organics pollutants were also investigated in the E-Fe-O process, and the influence of various water matrices on NB removal was discussed. All results demonstrated that the E-Fe-O process was an efficient method to remove refractory organic pollutants in various natural waters.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130264DOI Listing
August 2021

Binding between ROCK1 and DCTN2 triggers diabetes‑associated centrosome amplification in colon cancer cells.

Oncol Rep 2021 Jul 3;46(1). Epub 2021 Jun 3.

Institute of Biomedical Sciences of The School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.

Type 2 diabetes increases the risk various types of cancer and is associated with a poor prognosis therein. There is also evidence that the disease is associated with cancer metastasis. Centrosome amplification can initiate tumorigenesis with metastasis and increase the invasiveness of cancer cells . Our previous study reported that type 2 diabetes promotes centrosome amplification via the upregulation and centrosomal translocation of Rho‑associated protein kinase 1 (ROCK1), which suggests that centrosome amplification is a candidate biological link between type 2 diabetes and cancer development. In the present study, functional proteomics analysis was used to further investigate the molecular pathways underlying centrosome amplification by targeting ROCK1 binding partners. High glucose, insulin and palmitic acid were used to induce centrosome amplification, and immunofluorescent staining was employed to visualize centrosomal alterations. Combined with immunoprecipitation, mass spectrometry‑based proteomics analysis was used to identify ROCK1 binding proteins, and protein complex disruption was achieved by siRNA‑knockdown. In total, 1,148 ROCK1 binding proteins were identified, among which 106 proteins were exclusively associated with the treated samples, 193 were only associated with the control samples, and 849 were found in both the control and treated samples. Of the proteins with evidence of centrosomal localization, Dynactin subunit 2 (DCTN2) was confirmed to be localized to the centrosomes. Treating the cells with high glucose, insulin and palmitic acid increased the protein levels of ROCK1 and DCTN2, promoted their binding with each other, and triggered centrosome amplification. Disruption of the protein complex by knocking down ROCK1 or DCTN2 expression partially attenuated centrosome amplification, while simultaneous knockdown of both proteins completely inhibited centrosome amplification. These results suggested ROCK1‑DCTN2 binding as a signal for the regulation of centrosome homeostasis, which is key for diabetes‑associated centrosome amplification, and enriches our knowledge of centrosome biology. Therefore, the ROCK1‑DCTN2 complex may serve as a target for inhibiting centrosome amplification both in research or future therapeutic development.
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http://dx.doi.org/10.3892/or.2021.8102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185503PMC
July 2021

Elevating the Levels of Calcium Ions Exacerbate Alzheimer's Disease via Inducing the Production and Aggregation of β-Amyloid Protein and Phosphorylated Tau.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

College of Life and Health Sciences, Northeastern University, Shenyang 110819, China.

Alzheimer's disease (AD) is a neurodegenerative disease with a high incidence rate. The main pathological features of AD are β-amyloid plaques (APs), which are formed by β-amyloid protein (Aβ) deposition, and neurofibrillary tangles (NFTs), which are formed by the excessive phosphorylation of the tau protein. Although a series of studies have shown that the accumulation of metal ions, including calcium ions (Ca), can promote the formation of APs and NFTs, there is no systematic review of the mechanisms by which Ca affects the development and progression of AD. In view of this, the current review summarizes the mechanisms by which Ca is transported into and out of cells and organelles, such as the cell, endoplasmic reticulum, mitochondrial and lysosomal membranes to affect the balance of intracellular Ca levels. In addition, dyshomeostasis of Ca plays an important role in modulating the pathogenesis of AD by influencing the production and aggregation of Aβ peptides and tau protein phosphorylation and the ways that disrupting the metabolic balance of Ca can affect the learning ability and memory of people with AD. In addition, the effects of these mechanisms on the synaptic plasticity are also discussed. Finally, the molecular network through which Ca regulates the pathogenesis of AD is introduced, providing a theoretical basis for improving the clinical treatment of AD.
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http://dx.doi.org/10.3390/ijms22115900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198078PMC
May 2021

Genome-Wide and Comprehensive Analysis of the Multiple Stress-Related CAF1 (CCR4-Associated Factor 1) Family and Its Expression in Poplar.

Plants (Basel) 2021 May 14;10(5). Epub 2021 May 14.

Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

Poplar is one of the most widely used tree in afforestation projects. However, it is susceptible to abiotic and biotic stress. CCR4-associated factor 1 () is a major member of CCR4-NOT, and it is mainly involved in transcriptional regulation and mRNA degradation in eukaryotes. However, there are no studies on the molecular phylogeny and expression of the gene in poplar. In this study, a total of 19 genes were identified in the genome. Phylogenetic analysis of the gene family was performed with two closely related species ( and ) to investigate the evolution of the gene. The tissue expression of the gene showed that 19 genes were present in different tissues of poplar. Additionally, the analysis of the expression of the gene showed that the family was up-regulated to various degrees under biotic and abiotic stresses and participated in the poplar stress response. The results of our study provide a deeper understanding of the structure and function of the gene and may contribute to our understanding of the molecular basis of stress tolerance in poplar.
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http://dx.doi.org/10.3390/plants10050981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155972PMC
May 2021

Ectopic Overexpression of Histone H3K4 Methyltransferase CsSDG36 from Tea Plant Decreases Hyperosmotic Stress Tolerance in .

Int J Mol Sci 2021 May 11;22(10). Epub 2021 May 11.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Histone methylation plays an important regulatory role in the drought response of many plants, but its regulatory mechanism in the drought response of the tea plant remains poorly understood. Here, drought stress was shown to induce lower relative water content and significantly downregulate the methylations of histone H3K4 in the tea plant. Based on our previous analysis of the SET Domain Group () gene family, the full-length coding sequence (CDS) of was cloned from the tea cultivar ''. Bioinformatics analysis showed that the open reading frame (ORF) of the gene was 3138 bp, encoding 1045 amino acids and containing the conserved structural domains of PWWP, PHD, SET and PostSET. The CsSDG36 protein showed a close relationship to AtATX4 of the TRX subfamily, with a molecular weight of 118,249.89 Da, and a theoretical isoelectric point of 8.87, belonging to a hydrophilic protein without a transmembrane domain, probably located on the nucleus. The expression of was not detected in the wild type, while it was clearly detected in the over-expression lines of Arabidopsis. Compared with the wild type, the over-expression lines exhibited lower hyperosmotic resistance by accelerating plant water loss, increasing reactive oxygen species (ROS) pressure, and increasing leaf stomatal density. RNA-seq analysis suggested that the overexpression caused the differential expression of genes related to chromatin assembly, microtubule assembly, and leaf stomatal development pathways. qRT-PCR analysis revealed the significant down-regulation of stomatal development-related genes (, , , , , , , , and ) in the overexpression lines. This study provides a novel sight on the function of histone methyltransferase CsSDG36 under drought stress.
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http://dx.doi.org/10.3390/ijms22105064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150943PMC
May 2021

Rapid antimicrobial susceptibility testing by stimulated Raman scattering metabolic imaging and morphological deformation of bacteria.

Anal Chim Acta 2021 Jul 7;1168:338622. Epub 2021 May 7.

Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China. Electronic address:

Methods for rapid antimicrobial susceptibility testing (AST) are urgently needed to address the emergence and spread of antimicrobial resistance. Here, we report a new method based on stimulated Raman scattering (SRS) microscopy, which measures both the metabolic activity and the morphological deformation of bacteria to determine the antimicrobial susceptibility of β-lactam antibiotics rapidly. In this approach, we quantify single bacteria's metabolic activity by the carbon-deuterium (C-D) bond concentrations in bacteria after DO incubation. In the meantime, bacterial morphological deformation caused by β-lactam antibiotics is also measured. With these two quantifiable markers, we develop an evaluation method to perform AST of cefotaxime on 103 E. coli strains. Our method achieved a 93.2% categorical agreement and a 93.2% essential agreement with the standard reference method.
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http://dx.doi.org/10.1016/j.aca.2021.338622DOI Listing
July 2021

Nitric oxide down-regulates voltage-gated Na channel in cardiomyocytes possibly through S-nitrosylation-mediated signaling.

Sci Rep 2021 May 28;11(1):11273. Epub 2021 May 28.

Department of Pathophysiology, Oita University School of Medicine, Yufu, Oita, 879-5593, Japan.

Nitric oxide (NO) is produced from endothelial cells and cardiomyocytes composing the myocardium and benefits cardiac function through both vascular-dependent and-independent effects. This study was purposed to investigate the possible adverse effect of NO focusing on the voltage-gated Na channel in cardiomyocytes. We carried out patch-clamp experiments on rat neonatal cardiomyocytes demonstrating that NOC-18, an NO donor, significantly reduced Na channel current in a dose-dependent manner by a long-term application for 24 h, accompanied by a reduction of Nav1.5-mRNA and the protein, and an increase of a transcription factor forkhead box protein O1 (FOXO1) in the nucleus. The effect of NOC-18 on the Na channel was blocked by an inhibitor of thiol oxidation N-ethylmaleimide, a disulfide reducing agent disulfide 1,4-Dithioerythritol, or a FOXO1 activator paclitaxel, suggesting that NO is a negative regulator of the voltage-gated Na channel through thiols in regulatory protein(s) for the channel transcription.
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http://dx.doi.org/10.1038/s41598-021-90840-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163867PMC
May 2021

MD-UNET: Multi-input dilated U-shape neural network for segmentation of bladder cancer.

Comput Biol Chem 2021 Aug 20;93:107510. Epub 2021 May 20.

School of Information Science and Engineering, Shandong Normal University, Jinan, 250014, China. Electronic address:

Accurate segmentation of the tumour area is crucial for the treatment and prognosis of patients with bladder cancer. However, the complex information from the MRI image poses an important challenge for us to accurately segment the lesion, for example, the high distinction among people, size of bladder variation and noise interference. Based on the above issues, we propose an MD-Unet network structure, which uses multi-scale images as the input of the network, and combines max-pooling with dilated convolution to increase the receptive field of the convolutional network. The results show that the proposed network can obtain higher precision than the existing models for the bladder cancer dataset. The MD-Unet can achieve state-of-art performance compared with other methods.
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http://dx.doi.org/10.1016/j.compbiolchem.2021.107510DOI Listing
August 2021

Identification of nucleic acid aptamers against lactate dehydrogenase via SELEX and high-throughput sequencing.

Anal Bioanal Chem 2021 Jul 24;413(17):4427-4439. Epub 2021 May 24.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Nucleic acid aptamers are small fragments of DNA or RNA molecules binding specifically to targets, which can be obtained through in vitro screening via systematic evolution of ligands by exponential enrichment (SELEX). Lactate dehydrogenase (LDH) is an important tumor marker, whose level in patients is of great significance for diagnosis of many diseases. Here, we report the identification of LDH aptamers by 9 rounds of screening from a length-mixed single-stranded DNA library using the SELEX technology. After the 3rd and 7th rounds of aptamer screening, affinity was significantly improved, and fluorescence quantitative analysis showed stronger affinity for the aptamers selected from the 7th to 9th rounds of screening. After high-throughput sequencing, motif analysis, and secondary structure prediction, we finally chose and further investigated 15 candidate LDH aptamer sequences with obvious differences in secondary structure in the 7th to 9th rounds of screening. Among them, LDH7-1, LDH7-9, LDH8-2, and LDH9-1 were shown to bind to LDH protein with high affinity and specificity with K < 25 nM. This study provides new ideas for rapid detection of LDH protein content and enzyme activity, thus contributing to the development of rapid medical detection.
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http://dx.doi.org/10.1007/s00216-021-03397-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141549PMC
July 2021

STC1 ameliorates cognitive impairment and neuroinflammation of Alzheimer's disease mice via inhibition of ERK1/2 pathway.

Immunobiology 2021 May 8;226(3):152092. Epub 2021 May 8.

Department of Neurology, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei, China. Electronic address:

Objective: To investigate the regulatory role of STC1 (Stanniocalcin-1) mediated ERK1/2 pathway in cognitive impairment and neuroinflammation of Alzheimer's disease (AD).

Methods: WT mice and STC1 Tg mice (transgenic overexpression of STC1) were used to establish AD models to perform behavioral test by Morris water maze. Hippocampal cell apoptosis was quantified by TUNEL staining, the levels of inflammatory cytokines in serum and hippocampal tissues determined by ELISA, as well as oxidative stress-related factors detected by corresponding testing kits, and protein expression of STC1 and ERK1/2 pathway measured by Western blotting.

Results: Compared with WT Sham group, WT AD mice had prolonged escape latency, decreased crossing platform times, increased hippocampal cell apoptosis with up-regulated inflammatory cytokines and oxidative stress-related factors, as well as increased STC1 and ERK1/2 pathway-related molecules. By contrast, STC1 Tg AD mice showed shortened escape latency, increased crossing platform times than WT AD mice, and they also exhibited the decreased apoptosis index and inflammatory cytokines, alleviated oxidative stress-injury, down-regulated protein expression of ERK1/2 pathway, and up-regulated the protein expression of STC1 and UCP2.

Conclusion: STC1 overexpression could alleviate oxidative stress-induced injury, reduce neuroinflammation, improve cognitive function to play a neuro-protective role by inhibiting ERK1/2 signaling pathway.
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http://dx.doi.org/10.1016/j.imbio.2021.152092DOI Listing
May 2021

Bone marrow mesenchymal stem cells promote prostate cancer cell stemness via cell-cell contact to activate the Jagged1/Notch1 pathway.

Cell Biosci 2021 May 17;11(1):87. Epub 2021 May 17.

Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Mesenchymal stem cells (MSCs) play a crucial role in cancer development and tumor resistance to therapy in prostate cancer, but the influence of MSCs on the stemness potential of PCa cells by cell-cell contact remains unclear. In this study, we investigated the effect of direct contact of PCa cells with MSCs on the stemness of PCa and its mechanisms.

Methods: First, the flow cytometry, colony formation, and sphere formation were performed to determine the stemness of PCa, and the expression of stemness-related molecules (Sox2, Oct4, and Nanog) was investigated by western blot analysis. Then, we used western blot and qPCR to determine the activity levels of two candidate pathways and their downstream stemness-associated pathway. Finally, we verified the role of the significantly changed pathway by assessing the key factors in this pathway via in vitro and in vivo experiments.

Results: We established that MSCs promoted the stemness of PCa cells by cell-cell contact. We here established that the enhanced stemness of PCa was independent of the CCL5/CCR5 pathway. We also found that PCa up-regulated the expression of Notch signaling-related genes, and inhibition of Jagged1-Notch1 signaling in PCa cells significantly inhibited MSCs-induced stemness and tumorigenesis in vitro and in vivo.

Conclusions: Our results reveal a novel interaction between MSCs and PCa cells in promoting tumorigenesis through activation of the Jagged1/Notch1 pathway, providing a new therapeutic target for the treatment of PCa.
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http://dx.doi.org/10.1186/s13578-021-00599-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130143PMC
May 2021

Quantum random number generator with discarding-boundary-bin measurement and multi-interval sampling.

Opt Express 2021 Apr;29(8):12440-12453

A quantum random number generator (QRNG) provides a reliable means for the generation of true random numbers. The inherent randomness of the vacuum fluctuations makes the quantum vacuum state a superior source of entropy. However, in practice, the raw sequences of QRNG are inevitably contaminated by classical technical noise, which compromises the security of the QRNG. Min-entropy conditioned on the classical noise is a useful method that can quantify the side-information independent randomness. To improve the extractable randomness from the raw sequences arising from the quantum vacuum-based QRNG, we propose and experimentally demonstrate two approaches, discarding-boundary-bin measurement and multi-interval sampling. The first one increases the conditional min-entropy at a low quantum-to-classical-noise ratio. The latter exploits parallel sampling using multiple analog-to-digital converters (ADCs) and effectively overcomes the finite resolution limit and uniform sampling of a single ADC. The maximum average conditional min-entropy can reach 9.2 per sample when combining these two approaches together in contrast to 6.93 with a single 8-bit ADC.
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http://dx.doi.org/10.1364/OE.419756DOI Listing
April 2021

Effect of Cyclic Compressive Forces on New Bone Formation during the Distraction Period in Mandibular Distraction Osteogenesis Using a Microactuator-Generated Distractor.

Plast Reconstr Surg 2021 Jun;147(6):1072e-1073e

Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007947DOI Listing
June 2021

Effect of Low Intensity Transcranial Ultrasound Stimulation on Neuromodulation in Animals and Humans: An Updated Systematic Review.

Front Neurosci 2021 14;15:620863. Epub 2021 Apr 14.

Department of Neurology, Duke University School of Medicine, Durham, NC, United States.

Although low-intensity transcranial ultrasound stimulation (LI-TUS) has received more recognition for its neuromodulation potential, there remains a crucial knowledge gap regarding the neuromodulatory effects of LI-TUS and its potential for translation as a therapeutic tool in humans. In this review, we summarized the findings reported by recently published studies regarding the effect of LI-TUS on neuromodulation in both animals and humans. We also aim to identify challenges and opportunities for the translation process. A literature search of PubMed, Medline, EMBASE, and Web of Science was performed from January 2019 to June 2020 with the following keywords and Boolean operators: [transcranial ultrasound OR transcranial focused ultrasound OR ultrasound stimulation] AND [neuromodulation]. The methodological quality of the animal studies was assessed by the SYRCLE's risk of bias tool, and the quality of human studies was evaluated by the PEDro score and the NIH quality assessment tool. After applying the inclusion and exclusion criteria, a total of 26 manuscripts (24 animal studies and two human studies) out of 508 reports were included in this systematic review. Although both inhibitory (10 studies) and excitatory (16 studies) effects of LI-TUS were observed in animal studies, only inhibitory effects have been reported in primates (five studies) and human subjects (two studies). The ultrasonic parameters used in animal and human studies are different. The SYRCLE quality score ranged from 25 to 43%, with a majority of the low scores related to performance and detection bias. The two human studies received high PEDro scores (9/10). LI-TUS appears to be capable of targeting both superficial and deep cerebral structures to modulate cognitive or motor behavior in both animals and humans. Further human studies are needed to more precisely define the effective modulation parameters and thereby translate this brain modulatory tool into the clinic.
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http://dx.doi.org/10.3389/fnins.2021.620863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079725PMC
April 2021

Thirty Years Later: What Has Craniofacial Distraction Osteogenesis Surgery Replaced?

Plast Reconstr Surg 2021 May;147(5):892e-893e

Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007832DOI Listing
May 2021

Clinical study of atherosclerotic plaque in the carotid artery and lower extremities in patients with obstructive sleep apnea-hypopnea syndrome.

Clin Respir J 2021 Apr 17. Epub 2021 Apr 17.

Department of Neurology, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Hubei, China.

Objective: The present study aimed to explore the relationship between young and middle-aged patients with obstructive sleep apnea-hypopnea syndrome (OSAHS) and atherosclerotic plaque of the carotid artery and lower extremities.

Methods: A total of 110 patients diagnosed with OSAHS by polysomnography in the researchers' hospital from September 2018 to September 2019 were selected as the research objects. The enrolled patients were divided into a mild group (36 cases), a moderate group (38 cases), and a severe group (36 cases). Another 36 healthy subjects were set up as the non-OSAHS group. All the selected subjects were examined by color Doppler ultrasound of the carotid artery and lower extremity arteries.

Results: The detection rate of atherosclerotic plaque of the carotid artery and/or lower extremities in patients with OSAHS was significantly higher than that in the non-OSAHS group (P < 0.05), and the incidence of plaque tended to increase along with an increase in the degree of OSAHS.

Conclusion: The findings indicate that OSAHS might be an independent influencing factor for atherosclerosis.
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http://dx.doi.org/10.1111/crj.13378DOI Listing
April 2021

Katabatic Wind and Sea-Ice Dynamics Drive Isotopic Variations of Total Gaseous Mercury on the Antarctic Coast.

Environ Sci Technol 2021 05 15;55(9):6449-6458. Epub 2021 Apr 15.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Clarifying the sources and fates of atmospheric mercury (Hg) in the Antarctic is crucial to understand the global Hg circulation and its impacts on the fragile ecosystem of the Antarctic. Herein, the annual variations in the isotopic compositions of total gaseous Hg (TGM), with 5-22 days of sampling duration for each sample, were presented for the first time to provide isotopic evidence of the sources and environmental processes of gaseous Hg around the Chinese Great Wall Station (GWS) in the western Antarctic. Different from the Arctic tundra and lower latitude areas in the northern hemisphere, positive δHg (0.58 ± 0.21‰, mean ± 1SD) and negative ΔHg (-0.30 ± 0.10‰, mean ± 1SD) in TGM at the GWS indicated little impact from the vegetation-air exchange in the Antarctic. Correlations among TGM ΔHg, air temperature, and ozone concentrations suggested that enhanced katabatic wind that transported inland air masses to the continental margin elevated TGM ΔHg in the austral winter, while the surrounding marine surface emissions controlled by sea-ice dynamics lowered TGM ΔHg in the austral summer. The oxidation of Hg(0) might elevate ΔHg in TGM during atmospheric Hg depletion events but have little impact on the seasonal variations of atmospheric Hg isotopes. The presented atmospheric Hg isotopes were essential to identify the transport and transformation of atmospheric Hg and further understand Hg cycling in the Antarctic.
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http://dx.doi.org/10.1021/acs.est.0c07474DOI Listing
May 2021

Pump RIN coupling to frequency noise of a polarization-maintaining 2 µm single frequency fiber laser.

Opt Express 2021 Feb;29(3):3221-3229

We investigated the frequency noise coupling mechanism of a 2 μm polarization-maintaining single frequency fiber laser (SFFL) theoretically and experimentally. The coupling of pump's relative intensity noise (RIN) to frequency noise of a single-frequency high-gain silica fiber laser is shown experimentally to be consistent with a theoretical model where thermal expansion and thermo-optic effect mediate the coupling. The measured and theoretical frequency noise of the 2 μm SFFL with three pump sources is compared. We find using a 1550 nm single frequency laser pump source produces the lowest frequency noise, less than 100 / at frequencies higher than 100 Hz.
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http://dx.doi.org/10.1364/OE.415298DOI Listing
February 2021
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