Dr. Pramod Kumar Singh, Ph.D - King George's Medical University - To evaluate the role of omega-3 fatty acid against lead induced  neurotoxicity in Wistar rats: An experimental study

Dr. Pramod Kumar Singh

Ph.D

King George's Medical University

To evaluate the role of omega-3 fatty acid against lead induced neurotoxicity in Wistar rats: An experimental study

Lucknow, UP | India

Main Specialties: Biology, Clinical Neurophysiology, Medical Toxicology, Neuropathology

Additional Specialties: Biochemistry

ORCID logohttps://orcid.org/0000-0002-9352-2304


Top Author

Dr. Pramod Kumar Singh, Ph.D - King George's Medical University - To evaluate the role of omega-3 fatty acid against lead induced  neurotoxicity in Wistar rats: An experimental study

Dr. Pramod Kumar Singh

Ph.D

Introduction

Prof. R. K. Dixit
Department of Pharmacology & Therapeutics
King George’s Medical University, Lucknow- 226003
Uttar Pradesh, India
Phone no. +91- 9415089880
E-mail: rkdixit069@gmail.com

Dr. Manish Kumar Gupta
Assistant Professor
Department of Biotechnology
Veer Bahadur Singh Purvanchal University, Jaunpur-222003
Uttar Pradesh, India
Phone no. +91-9307267812
E-mail: manish.kumar.gupta@gmail.com

Primary Affiliation: King George's Medical University - Lucknow, UP , India

Specialties:

Additional Specialties:

Research Interests:


View Dr. Pramod Kumar Singh’s Resume / CV

Education

May 2016
King George s Medical University, Lucknow
Ph.D.
Medical Biochemistry
Sep 2010
Bundelkhand University, Jhansi
M.Sc.
Biochemistry
Jun 2006
V.B.S. Purvanchal University, Jaunpur
B.Sc.
Biology

Experience

Nov 2019
Scopolamine induced amnesia
Post-Doctoral Fellow (PUPDF)
Department of Biochemistry V.B.S. Purvanchal University, Jaunpur
Oct 2018
Clinical demonstration to patient
Demonstrator/Tutor
Department of Biochemistry All India Institute of Medical Sciences, Bhopal 462020 M.P. India
Nov 2016
Role of omega-3 fatty acid against chronic lead induced neurotoxicity
Senior Research Fellow
Department of Pharmacology
Dec 2013
Role of omega-3 fatty acid against chronic lead induced neurotoxicity
Senior Research Fellow
Department of Pharmacology
Dec 2013
Role of omega-3 fatty acid against chronic lead induced neurotoxicity
Senior Research Fellow
Department of Pharmacology
Dec 2013
Role of omega-3 fatty acid against chronic lead induced neurotoxicity
Senior Research Fellow
Department of Pharmacology
Mar 2013
Studies to evaluate the toxicity profile of UNIM-105 and UNIM-115 polyherbal Unani oral formulations with therapeutics potential in viral hepatitis
Senior Research Fellow
Department of Biochemistry
Sep 2011
Effect of lead toxicity in rats brain
Research Fellow
Department of Biochemistry

Publications

11Publications

28Reads

74Profile Views

1PubMed Central Citations

Cinnamon zeylanicum attenuates oxidative stress and improves neurobehavioral activity in lead induced neurotoxicity

International Journal of Current Research

The present study has been carried out to investigate the neuroprotective activity of cinnamon (Cinnamon zeylanicumin) on lead induced neurotoxicity and behavioral impairments in rats. Different behavioral parameters and biochemical assays in brain of rats were observed. Rats exposed to lead (lead acetate 5.0mg/kg body weight p.o. for 28 days) caused a significant decrease in body weight, brain weight and behavioral changes as compared to controls. The increased levels of lead in blood and brain also increases the levels of ROS, LPO and decreases the levels of GSH with concomitant reduction in SOD, CAT and GPx activities in brain of rats treated with lead as compared to controls. Co-treatment of lead with cinnamon oil (75mg/kg body weight p.o. for 28 days) decreases the levels of ROS, LPO and increases the level of GSH, SOD, CAT and GPx activity and showed improvements in behavioral changes as compared to lead treated groups. The results obtained were compared with vitamin E (100 mg/kg body weight p.o. for 28 days)as the standard antioxidant drug. Our results suggested that, cinnamon oil causes improvement in behavioral deficits and oxidative stress similar to that of standard drug, vitamin-E. This work reveals the potential of cinnamon oil as a protective drug for lead induced neurotoxicity and associated human health risk.

View Article
March 2018
2 Reads

Cinnamon zeylanicum attenuates oxidative stress and improves neurobehavioral activity in lead induced neurotoxicity

International Journal of Current Research

The present study has been carried out to investigate the neuroprotective activity of cinnamon (Cinnamon zeylanicumin) on lead induced neurotoxicity and behavioral impairments in rats. Different behavioral parameters and biochemical assays in brain of rats were observed. Rats exposed to lead (lead acetate 5.0mg/kg body weight p.o. for 28 days) caused a significant decrease in body weight, brain weight and behavioral changes as compared to controls. The increased levels of lead in blood and brain also increases the levels of ROS, LPO and decreases the levels of GSH with concomitant reduction in SOD, CAT and GPx activities in brain of rats treated with lead as compared to controls. Co-treatment of lead with cinnamon oil (75mg/kg body weight p.o. for 28 days) decreases the levels of ROS, LPO and increases the level of GSH, SOD, CAT and GPx activity and showed improvements in behavioral changes as compared to lead treated groups. The results obtained were compared with vitamin E (100 mg/kg body weight p.o. for 28 days)as the standard antioxidant drug. Our results suggested that, cinnamon oil causes improvement in behavioral deficits and oxidative stress similar to that of standard drug, vitamin-E. This work reveals the potential of cinnamon oil as a protective drug for lead induced neurotoxicity and associated human health risk.

View Article
March 2018
1 Read

Cinnamon zeylanicum attenuates oxidative stress and improves neurobehavioral activity in lead induced neurotoxicity

International Journal of Current Research

The present study has been carried out to investigate the neuroprotective activity of cinnamon (Cinnamon zeylanicumin) on lead induced neurotoxicity and behavioral impairments in rats. Different behavioral parameters and biochemical assays in brain of rats were observed. Rats exposed to lead (lead acetate 5.0mg/kg body weight p.o. for 28 days) caused a significant decrease in body weight, brain weight and behavioral changes as compared to controls. The increased levels of lead in blood and brain also increases the levels of ROS, LPO and decreases the levels of GSH with concomitant reduction in SOD, CAT and GPx activities in brain of rats treated with lead as compared to controls. Co-treatment of lead with cinnamon oil (75mg/kg body weight p.o. for 28 days) decreases the levels of ROS, LPO and increases the level of GSH, SOD, CAT and GPx activity and showed improvements in behavioral changes as compared to lead treated groups. The results obtained were compared with vitamin E (100 mg/kg body weight p.o. for 28 days)as the standard antioxidant drug. Our results suggested that, cinnamon oil causes improvement in behavioral deficits and oxidative stress similar to that of standard drug, vitamin-E. This work reveals the potential of cinnamon oil as a protective drug for lead induced neurotoxicity and associated human health risk.

View Article
March 2018
1 Read

Cinnamon zeylanicum attenuates oxidative stress and improves neurobehavioral activity in lead induced neurotoxicity

International Journal of Current Research

The present study has been carried out to investigate the neuroprotective activity of cinnamon (Cinnamon zeylanicumin) on lead induced neurotoxicity and behavioral impairments in rats. Different behavioral parameters and biochemical assays in brain of rats were observed. Rats exposed to lead (lead acetate 5.0mg/kg body weight p.o. for 28 days) caused a significant decrease in body weight, brain weight and behavioral changes as compared to controls. The increased levels of lead in blood and brain also increases the levels of ROS, LPO and decreases the levels of GSH with concomitant reduction in SOD, CAT and GPx activities in brain of rats treated with lead as compared to controls. Co-treatment of lead with cinnamon oil (75mg/kg body weight p.o. for 28 days) decreases the levels of ROS, LPO and increases the level of GSH, SOD, CAT and GPx activity and showed improvements in behavioral changes as compared to lead treated groups. The results obtained were compared with vitamin E (100 mg/kg body weight p.o. for 28 days)as the standard antioxidant drug. Our results suggested that, cinnamon oil causes improvement in behavioral deficits and oxidative stress similar to that of standard drug, vitamin-E. This work reveals the potential of cinnamon oil as a protective drug for lead induced neurotoxicity and associated human health risk.

View Article
March 2018
1 Read

Cinnamon zeylanicum attenuates oxidative stress and improves neurobehavioral activity in lead induced neurotoxicity

International Journal of Current Research

The present study has been carried out to investigate the neuroprotective activity of cinnamon (Cinnamon zeylanicumin) on lead induced neurotoxicity and behavioral impairments in rats. Different behavioral parameters and biochemical assays in brain of rats were observed. Rats exposed to lead (lead acetate 5.0mg/kg body weight p.o. for 28 days) caused a significant decrease in body weight, brain weight and behavioral changes as compared to controls. The increased levels of lead in blood and brain also increases the levels of ROS, LPO and decreases the levels of GSH with concomitant reduction in SOD, CAT and GPx activities in brain of rats treated with lead as compared to controls. Co-treatment of lead with cinnamon oil (75mg/kg body weight p.o. for 28 days) decreases the levels of ROS, LPO and increases the level of GSH, SOD, CAT and GPx activity and showed improvements in behavioral changes as compared to lead treated groups. The results obtained were compared with vitamin E (100 mg/kg body weight p.o. for 28 days)as the standard antioxidant drug. Our results suggested that, cinnamon oil causes improvement in behavioral deficits and oxidative stress similar to that of standard drug, vitamin-E. This work reveals the potential of cinnamon oil as a protective drug for lead induced neurotoxicity and associated human health risk.

View Article
March 2018
3 Reads

Omega-3 fatty acid attenuates oxidative stress in cerebral cortex, cerebellum and hippocampus tissue and improves neurobehavioral activity in chronic lead induced neurotoxicity

Nutritional Neuroscience

Abstract

Objectives: In view of the increasing risk of lead on human health, the present study has been carried out to investigate the neuroprotective effect of omega-3 fatty acid on chronic lead-induced neurotoxicity and behavioral impairment in rats. Methods: Different neurobehavioral parameters, biochemical assays, and histopathological analyses in brain regions of rats were conducted. Results: Rats exposed to different doses of lead (lead acetate 2.5, 5.0, 7.5 mg/kg body weight p.o. for 90 days) caused a significant decrease in body weight, brain weight, and behavioral changes as compared to controls. Abnormal histopathological and increased levels of lead in blood and brain regions increased the levels of ROS, LPO, PCC and decreased the levels of GSH with concomitant reduction in SOD, CAT, and GPx activities in the brain region of rats treated with different doses of lead as compared to controls. Co-treatment of lead with omega-3 fatty acid (500 mg/kg body weight p.o. for 90 days) decreased the levels of ROS, LPO, PCC, and increased the level of GSH, also increased SOD, CAT, and GPx activity and showed improvements in behavioral as well as histopathological changes as compared to lead-treated groups. Discussion: Our results proved that omega-3 fatty acid improved behavioral deficits, altered histopathological and oxidative stress in lead-intoxicated rats. Among three different doses, 2.5 mg/kg b.wt. of lead along with omega-3 fatty acid was the most preventive dose for the neurotoxicity. This work reveals the potential of omega-fatty acid as a protective drug for lead neurotoxicity.

View Article
April 2017
2 Reads

Attenuation of lead induced neurotoxicity by omega-3 fatty acid in rats.

Annals of Neurosciences

Background: Lead is widely distributed in the environment and has been found to be associated with the various health problems including neurodegenerative diseases. Purpose: In view of increasing health risk of lead, the study has been carried out to investigate the neuroprotective effect of omega-3 fatty acid in lead induced neurotoxicity in rats. Methods: Biochemical parameters including oxidative stress in brain regions, lead levels in blood and brain regions and histopathological examination of brain regions of rats were carried out in the present study. Results: Rats exposed to lead (lead acetate 7.5 mg/kg body weight p.o. for 14 days) caused a significant increase in the lipid peroxidation, ROS production and decreased levels of reduced glutathione, activity of superoxide dismutase and catalase in the cerebellum and cerebral cortex respectively as compared to controls. Abnormal histopathological changes and increase in the levels of lead in blood and brain were also observed as compared to controls. Co-treatment of lead with omega-3 fatty acid (750 mg/kg body weight p.o. for 14 days) decreased the levels of lipid peroxidation, ROS production and increased the levels of reduced glutathione, superoxide dismutase and catalase and showed protection in histopathological study as compared to rats treated with lead alone. Conclusions: The result of the present study exhibits that lead induced oxidative stress and histopathological alteration in brain regions significantly protected following co-treatment of lead and omega-3 fatty acid that could be due to its strong antioxidant potential and metal binding property.

View Article
April 2017
18 Reads

Attenuation of lead neurotoxicity by supplementation of polyunsaturated fatty acid in Wistar rats.

Nutr Neurosci 2016 Nov 25;19(9):396-405. Epub 2015 May 25.

a Department of Pharmacology and Therapeutics , King George's Medical University , Lucknow 226003 , India.

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Source
http://dx.doi.org/10.1179/1476830515Y.0000000028DOI Listing
November 2016
28 Reads
1 Citation
2.114 Impact Factor

Behavioral and Neurological effects of omega-3 fatty acid against lead acetate exposure in male wistar rats: An experimental study

Int J Curr Res. 2015 Sep 30; 7 (9): 20936-44

International Journal of Current Research

Omega-3 fatty acids are potent antioxidants and exhibit a biological activities including neuroprotective, anti-oxidant, anti-apoptotic and anti-inflammatory properties, and have been suggested to be useful in treatment of several diseases. The present study has been undertaken to investigate the protective effect of omega-3 fatty acid (750mg/kg bwt.) against lead acetate (7.5mg/kg bwt.) induced impairment in neurotransmitters and neurobehavioral in wistar rats. The levels of dopamine (DA), nor-epinephrine (NE) and serotonin (5-HT) were evaluated in brain regions (cerebral cortex and cerebellum) of adult male wistar rats. The result showed that the administration of acute dose of lead acetate (7.5 mg/kg bwt.) induced a significant (P<0.05) decrease levels of DA, NE, and 5-HT in the brain region. Treatment of rats with omega-3 fatty acid produced an improvement in most of the studied parameters as well as the neurobehavioral features. In conclusion our data showed that dietary omega-3 fatty acid provide protection on lead-induced behavioral and neurological effects.

View Article
September 2015
21 Reads

Effect of omega-3 fatty acid on lead induced general and behavioral profile in male wistar rats: An experimental study.

Int J Curr Res. 2014 Oct 25; 6 (10),:9304—08.

International Journal of Current Research

View Article
October 2014
25 Reads

Top co-authors

Rakesh Kumar Dixit
Rakesh Kumar Dixit

King George's Medical University

1
Suresh Babu
Suresh Babu

King George's Medical University

1
Akash Rawat
Akash Rawat

King George's Medical University

1
Mohammad Kaleem Ahmad
Mohammad Kaleem Ahmad

King George's Medical University

1
Rajendra Nath
Rajendra Nath

GSVM Medical College

1