Publications by authors named "Pradipta Mukherjee"

2 Publications

  • Page 1 of 1

Optical coherence tomography-based tissue dynamics imaging for longitudinal and drug response evaluation of tumor spheroids.

Biomed Opt Express 2020 Nov 8;11(11):6231-6248. Epub 2020 Oct 8.

Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki, Japan.

We present optical coherence tomography (OCT)-based tissue dynamics imaging method to visualize and quantify tissue dynamics such as subcellular motion based on statistical analysis of rapid-time-sequence OCT signals at the same location. The analyses include logarithmic intensity variance (LIV) method and two types of OCT correlation decay speed analysis (OCDS). LIV is sensitive to the magnitude of the signal fluctuations, while OCDSs including early- and late-OCDS (OCDS and OCDS , respectively) are sensitive to the fast and slow tissue dynamics, respectively. These methods were able to visualize and quantify the longitudinal necrotic process of a human breast adenocarcinoma spheroid and its anti-cancer drug response. Additionally, the effects of the number of OCT signals and the total acquisition time on dynamics imaging are examined. Small number of OCT signals, e.g., five or nine suffice for dynamics imaging when the total acquisition time is suitably long.
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http://dx.doi.org/10.1364/BOE.404336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687946PMC
November 2020

Quantitative discrimination of biological tissues by micro-elastographic measurement using an epi-illumination Mueller matrix microscope.

Biomed Opt Express 2019 Aug 9;10(8):3847-3859. Epub 2019 Jul 9.

Graduate School of Engineering, Utsunomiya University, Utsunomiya, Tochigi, 321-8585, Japan.

We propose a method for estimating the stiffness of bio-specimens by measuring their linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can be used to distinguish between specimens and quantify the discrimination accuracy.
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http://dx.doi.org/10.1364/BOE.10.003847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701520PMC
August 2019