Publications by authors named "Pouya Goleij"

10 Publications

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Identification of a compound heterozygous missense mutation in LAMA2 gene from a patient with merosin-deficient congenital muscular dystrophy type 1A.

J Clin Lab Anal 2021 Sep 16:e23930. Epub 2021 Sep 16.

Department of Medical Genetics, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Background: Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is occurred by mutations in LAMA2 gene that encodes the laminin α2 chain (merosin). MDC1A is a predominant subtype of congenital muscular dystrophy. Herein, we identified two missense mutations in LAMA2 gene in compound heterozygous status in an Iranian patient with MDC1A using whole-exome sequencing (WES).

Methods: In the present study, we evaluated genetic alterations in an Iranian 35-month-old boy with MDC1A and his healthy family using WES method. The identified mutations further confirmed by Sanger sequencing method. Finally, in silico analysis was conducted to further evaluation of molecular function of the identified genetic variants.

Results: We identified two potentially pathogenic missense mutations in compound heterozygous state (c.7681G>A p.Gly2561Ser and c.4840A>G p.Asn1614Asp) in LAMA2 gene as contributing to the MDC1A phenotype. The healthy parents of our proband are single heterozygous for identified mutations. These variants were found to be pathogenic by in silico analysis.

Conclusions: In general, we successfully identified LAMA2 gene mutations in an Iranian patient with MDC1A using WES. The identified mutations in LAMA2 gene can be useful in genetic counseling, prenatal diagnosis, and predicting prognosis of MDC1A.
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http://dx.doi.org/10.1002/jcla.23930DOI Listing
September 2021

Electrospun gold nanorods/graphene oxide loaded-core-shell nanofibers for local delivery of paclitaxel against lung cancer during photo-chemotherapy method.

Eur J Pharm Sci 2021 Sep 17;164:105914. Epub 2021 Jun 17.

Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran. Electronic address:

The combinations of photothermal therapy (PTT) and chemotherapy (CHT) have attracted increasing attention for cancer therapy. In the present study, paclitaxel as an anticancer drug and graphene oxide/gold nanorods (GO/Au NRs) were simultaneously loaded into the poly (tetramethylene ether) glycol based-polyurethane (PTMG-PU) (core)/chitosan (shell) nanofibers prepared by the coaxial electrospinning method. The potential of the synthesized nanofiber as a pH/temperature dual responsive carrier was investigated for the controlled release of paclitaxel against A549 lung cancer during PTT/CHT combined method. The synthesized core-shell nanofibers were characterized using SEM, TEM and XRD analysis. The drug encapsulation efficiency, drug release and kinetic studies were carried out. The compatibility of the synthesized core-shell nanofibers was also investigated. The cell viability of the synthesized nanofibers treated with A549 lung cancer cells was investigated under alone CHT, alone PTT and PTT/CHT method. The in vivo studies indicated that the PTT/CHT method demonstrated an optimal therapeutic effect on tumor inhibition without change in body weight. The obtained results demonstrated that the synthesized core-shell nanofibers would be used for lung cancer treatment under NIR irradiation in the future.
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http://dx.doi.org/10.1016/j.ejps.2021.105914DOI Listing
September 2021

Magnetic bioactive glasses/Cisplatin loaded-chitosan (CS)-grafted- poly (ε-caprolactone) nanofibers against bone cancer treatment.

Carbohydr Polym 2021 Apr 22;258:117680. Epub 2021 Jan 22.

Department of Materials and Metallurgical Engineering, Amirkabir University of Technology, Tehran, Iran.

The bioactive glasses (BGs)/Cisplatin and magnetic bioactive glasses (MBGs)/Cisplatin were doped into the chitosan (CS)-grafted- poly (ε-caprolactone) (PCL) nanofibers for controlled release of Cisplatin under various pH values and temperatures. The simultaneous effect of chemotherapy and hyperthermia was investigated against MG-63 osteosarcoma cells by treating of cells with Cs-g-PCL/MBGs/Cisplatin under an alternating magnetic field. The synthesized nanofibers were characterized using XRD, FTIR, H NMR, SEM, and EDX analysis. The bioactivity, and drug loading efficiency of fibers were investigated. There was no initial burst release of Cisplatin from BGs/Cisplatin and MBGs/Cisplatin loaded Cs-g-PCL/MBGs nanofibers and the Cisplatin release rate was accelerated under pH of 5.5 and temperature of 43 °C compared with physiological condition. The apoptotic/necrotic effect indicated that 100 μg mL nanofibers was optimum for killing of MG-63 cells. The future researches could be focused on the application of nanofibers as an implantable device next to a bone tumor for bone cancer therapy in vivo.
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http://dx.doi.org/10.1016/j.carbpol.2021.117680DOI Listing
April 2021

The Role of Non-coding Genome in Cancer-associated Fibroblasts; Stateof- the-Art and Perspectives in Cancer Targeted Therapy.

Curr Drug Targets 2021 ;22(13):1524-1535

Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran.

Cancer-associated fibroblasts (CAFs) are senescent fibroblasts in tumor nest, which trigger a signaling center to remodel a desmoplastic tumor niche. CAF's functions in cancer are closely similar to myofibroblasts during the wound healing process. They can produce cytokines, enzymes, and protein- or RNA-containing exosomes to alter the function of surrounding cells. Non-- coding RNAs, including microRNAs and long non-coding RNAs, modulate pathologic mechanisms in cancer. Dysregulation of these RNAs influences the formation and function of CAFs. Furthermore, it has been demonstrated that CAFs, by releasing non-coding RNAs-containing exosomes, affect the tumor cells' behavior. CAFs also secrete mediators such as chemokines to alter the expression of non-coding RNAs in the tumor microenvironment. This study aimed to discuss the role of non-coding RNAs in CAF development in cancer. Additionally, we have shed light on the therapeutic approaches to develop the strategies based on the alteration of non-coding RNAs in cancer.
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http://dx.doi.org/10.2174/1389450122666210216091953DOI Listing
January 2021

Melatonin increases the anticancer potential of doxorubicin in Caco-2 colorectal cancer cells.

Environ Toxicol 2021 Jun 28;36(6):1061-1069. Epub 2021 Jan 28.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Colorectal cancer (CC) is an important human malignancy with high cancer related death worldwide. The chemotherapy using doxorubicin hydrochloride is one of the most common cancer therapeutic methods. However, drug resistance lowers the treatment efficacy in CC patients. The combination therapies seem to be more promising by taking the advantage of synergistic effects. The present study aimed to evaluate a new strategy to enhance the anticancer activity of doxorubicin in Caco-2 CC cell line by co-administration of melatonin. The effects of doxorubicin, melatonin, and their combinations (Dox-Mel) were investigated on the proliferation and viability, morphological alterations, and tumor spheroid formation. Flow cytometry was employed to compare the apoptotic situation of the cells in study groups. Changes in metastatic potential of the cells were assessed by wound healing assay and trans-well migration assays. Moreover, expression of BAX, SMAC, BCL-2, SURVIVIN, MMP-2, and MMP-9 genes were evaluated by quantitative real time PCR and western blotting. Our study showed that doxorubicin, melatonin, and Dox-Mel significantly decreased the proliferation and viability, tumor spheroid formation, invasion, and migration. Furthermore, the changes were in a concentration and time dependent manner. There was an increase in apoptosis rate in the treatment groups. Expression of genes involved in apoptosis and cell motility were altered significantly. It was observed that anticancer activity of Dox-Mel combination was significantly more than doxorubicin and melatonin treatments alone. We showed an enhanced apoptotic and anticancer activity of doxorubicin and melatonin combination chemotherapy on CC cell line than doxorubicin or melatonin treatments alone. This combination could promote the treatment efficiency and alleviate the un-intended side effects by lowering the dose of doxorubicin prescription.
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http://dx.doi.org/10.1002/tox.23105DOI Listing
June 2021

Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA-PAA nano-encapsulation on PANC-1 pancreatic cancer cell line.

Environ Toxicol 2021 Jun 26;36(6):1043-1051. Epub 2021 Jan 26.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on PANC-1 cell line. The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cells. We observed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC-1 cells. According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cells than free Hyd or Hyd monotherapy.
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http://dx.doi.org/10.1002/tox.23103DOI Listing
June 2021

Effects of therapeutic probiotics on modulation of microRNAs.

Cell Commun Signal 2021 01 11;19(1). Epub 2021 Jan 11.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.

Probiotics are beneficial bacteria that exist within the human gut, and which are also present in different food products and supplements. They have been investigated for some decades, due to their potential beneficial impact on human health. Probiotics compete with pathogenic microorganisms for adhesion sites within the gut, to antagonize them or to regulate the host immune response resulting in preventive and therapeutic effects. Therefore, dysbiosis, defined as an impairment in the gut microbiota, could play a role in various pathological conditions, such as lactose intolerance, gastrointestinal and urogenital infections, various cancers, cystic fibrosis, allergies, inflammatory bowel disease, and can also be caused by antibiotic side effects. MicroRNAs (miRNAs) are short non-coding RNAs that can regulate gene expression in a post-transcriptional manner. miRNAs are biochemical biomarkers that play an important role in almost all cellular signaling pathways in many healthy and disease states. For the first time, the present review summarizes current evidence suggesting that the beneficial properties of probiotics could be explained based on the pivotal role of miRNAs. Video Abstract.
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http://dx.doi.org/10.1186/s12964-020-00668-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798223PMC
January 2021

The role of non-coding genome in the behavior of infiltrated myeloid-derived suppressor cells in tumor microenvironment; a perspective and state-of-the-art in cancer targeted therapy.

Prog Biophys Mol Biol 2021 05 28;161:17-26. Epub 2020 Nov 28.

Student Research Committee, Bam University of Medical Sciences, Bam, Iran; Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran. Electronic address:

Cancer is one of the healthcare problems that affect many communities around the world. Many factors contribute to cancer development. Besides, these factors are counted as the main impediment in cancer immunotherapy. Myeloid-derived suppressor cells (MDSCs) are one of these impediments. MDSCs inhibit the immune responses through various mechanisms such as inhibitory cytokine release and nitric oxide metabolite production. Several factors are involved in forming these cells, including tumor secreted cytokine and chemokines, transcription factors, and non-coding RNA. In the meantime, micro-RNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the vital gene regulatory elements that affect gene expression. In this study, we are going to discuss the role of miRNAs and lncRNAs in MDSCs development in a cancer situation. It is hoped that miRNA and lncRNAs targeting may prevent the growth and development of these inhibitory cells in the cancer environment.
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http://dx.doi.org/10.1016/j.pbiomolbio.2020.11.006DOI Listing
May 2021

Synthesis of PLGA/chitosan/zeolites and PLGA/chitosan/metal organic frameworks nanofibers for targeted delivery of Paclitaxel toward prostate cancer cells death.

Int J Biol Macromol 2020 Dec 28;164:1461-1474. Epub 2020 Jul 28.

Department of Materials and Metallurgical Engineering, Amirkabir University of Technology, Tehran, Iran.

In the present study, the various zeolites including hydrophilic Y zeolite, hydrophobic ZSM-5 zeolite and metal organic frameworks (MOFs) including MIL-101 and ZIF-8 were incorporated into the PLGA/chitosan nanofibers for controlled release of Paclitaxel anticancer drug against prostate cancer in vitro and in vivo. The synthesized nanoparticles and nanofibers were characterized using FTIR, XRD, SEM, BET and water contact angle analysis. The drug loading efficiency of nanofibers containing zeolites and MOFs indicated that the MOFs were more useful compared with zeolites for higher loading of Paclitaxel molecules. The Paclitaxel release behavior from nanofibers containing zeolites and MOFs were also examined. The MTT assay and DAPI staining analysis were used to determine the cytotoxicity and apoptosis effect of nanofibers containing Paclitaxel against LNCaP prostate cancer cell lines. The tumor inhibition rate in vivo was carried out to obtain the optimum nanofibrous formulation with maximum cell death percentage and tumor inhibition rate. The obtained results revealed the better activity of MOFs compared nanozeolites for higher loading of Paclitaxel drug into the nanoparticles and a more sustained release of drug from nanofibers containing MOFs.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.228DOI Listing
December 2020

ASSOCIATION BETWEEN DIABETICS AND INTESTINAL CANCER WITH THE RISK OF MUTATION IN CD38 GENE IN IRANIAN POPULATION.

Arq Gastroenterol 2020 Apr-Jun;57(2):137-143

Islamic Azad University, Faculty of Biological Sciences, Department of Genetics, Tonekabon Branch, Iran.

Background: Intestinal cancer often occurs in type 2 diabetic patients. The concept of increasing insulin levels and insulin-like growth factor in the blood with type 2 diabetes are stimulated with the growth and depletion of cloned cell walls, and the continuation of this process leads to the cellular deformation. This is the evidence for intestinal cancer in type 2 diabetes in population.

Objective: In this study, we aimed to find out the relationship between diabetics and intestinal cancer based on CD38 gene mutation.

Methods: Samples were collected from 200 population including normal and case ones. PCR products related to rs 6449181 of CD38 gene was amplified with ARMS-PCR technique, and a 420-bp sharp banding was observed as well. According three ARMS-PCR techniques, three primers were designed by oligo7 software. Primers include F1, F2 and R (amplifying for normal, mutant and reverse primer respectively).

Results: This band was observed using a primer F1 that carries the wild type nucleotide using a primer, and when it is used with the F2 primer, it brings the mutant primer to populations of patients with diabetes and diabetes-cancer. In addition, the clinical results including body mass index, blood glucose and insulin level were analyzed. The means ±SD and Tuckey's post hoc test were significant between the clinical characterization parameters between cases and healthy populations. The allelic gene frequencies and Hardy-Weinberg equilibrium between nucleotides were evaluated, and the significant level between the alleles and gene frequencies was observed.

Conclusion: In general, the current study found that there is a relationship between diabetes and intestinal cancer among the studied populations.
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http://dx.doi.org/10.1590/s0004-2803.202000000-25DOI Listing
September 2020
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