Publications by authors named "Piotr Ossowski"

8 Publications

  • Page 1 of 1

Nutritional Strategies for the Individualized Treatment of Non-Alcoholic Fatty Liver Disease (NAFLD) Based on the Nutrient-Induced Insulin Output Ratio (NIOR).

Int J Mol Sci 2016 Jul 22;17(7). Epub 2016 Jul 22.

Liver and Internal Medicine Unit, Department of General, Transplant and Liver Surgery of the Medical University of Warsaw, Warsaw 02-097, Poland.

Nutrients play a fundamental role as regulators of the activity of enzymes involved in liver metabolism. In the general population, the action of nutrients may be affected by gene polymorphisms. Therefore, individualization of a diet for individuals with fatty liver seems to be a fundamental step in nutritional strategies. In this study, we tested the nutrient-induced insulin output ratio (NIOR), which is used to identify the correlation between the variants of genes and insulin resistance. We enrolled 171 patients, Caucasian men (n = 104) and women (n = 67), diagnosed with non-alcoholic fatty liver disease (NAFLD). From the pool of genes sensitive to nutrient content, we selected genes characterized by a strong response to the NIOR. The polymorphisms included Adrenergic receptor (b3AR), Tumor necrosis factor (TNFα), Apolipoprotein C (Apo C III). Uncoupling Protein type I (UCP-1), Peroxisome proliferator activated receptor γ2 (PPAR-2) and Apolipoprotein E (APOEs). We performed three dietary interventions: a diet consistent with the results of genotyping (NIOR (+)); typical dietary recommendations for NAFLD (Cust (+)), and a diet opposite to the genotyping results (NIOR (-) and Cust (-)). We administered the diet for six months. The most beneficial changes were observed among fat-sensitive patients who were treated with the NIOR (+) diet. These changes included improvements in body mass and insulin sensitivity and normalization of blood lipids. In people sensitive to fat, the NIOR seems to be a useful tool for determining specific strategies for the treatment of NAFLD.
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http://dx.doi.org/10.3390/ijms17071192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964561PMC
July 2016

[Carbon monoxide poisoning in pregnant woman].

Przegl Lek 2015 ;72(9):482-4

Carbon monoxide poisoning is one of the most frequent types of poisoning caused by gases. Exposure of a pregnant woman to carbon monoxide is connected with transmission of this gas to her fetus what may cause oxygen deficiency, and further, the damage to its organs. The article describes a case of carbon monoxide poisoning of a 28-weeks pregnant woman who was treated in a hyperbaric chamber. Therapy was successful and the woman gave birth to a healthy, full term infant. In case of poisoning to a pregnant woman the above seems to be the most advantageous solution.
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April 2016

Metabolic risk factors of coronary heart disease in relation to anthropometric measures in nonalcoholic fatty liver disease patients following dietary intervention.

Pomeranian J Life Sci 2016;62(2):8-14

Introduction: Nonalcoholic fatty liver disease (NAFLD) is connected with high risk of metabolic disorders, such as: metabolic syndrome, type 2 diabetes, dyslipidemia and independently, coronary heart disease (CHD).

Materials And Methods: A group of 108 Caucasian individuals (39 women and 69 men) aged 20–77 years with NAFLD were prospectively enrolled in the study. The diagnosis of NAFLD was con- 􀏐irmed during the 􀏐irst appointment using ultrasonography. The liver serum tests such as alanine aminotransferase and aspartate aminotransferase levels were taken into account, as well. All of the tests and measurements were taken at the begining and after 6 month of dieting. Anthropometric tools (body mass index – BMI, waist circumference – WC, waist to hip ratio – WHR, waist to height ratio – WHtR, coinicity index – C -Index) were examined in relation to metabolic risk factors of CHD (increased: triacylglycerols, low density lipoprotein, total cholesterol, glycemia and low high density lipoprotein).

Results: Statistical signi􀏐icant reduction of body weight, BMI, WC, WHR, WHtR and C -Index (p < 0.05) contributed to the improvement of serum triacylglycerols in both sexes (p < 0.05). Indicators of abdominal obesity (WC, WHR, WHtR, C -Index) correlated signi􀏐icantly with serum glucose (p < 0.05) both before and after the diet and with serum lipids, either before or after the diet (p < 0.05), in both sexes.

Conclusions: It seems bene􀏐icial to spread the use of anthropometric tools, especially C -Index and WHtR, in primary health care practice for either early prevention or prediction of metabolic CHD risk occurrence, and its potential fatal consequences among patients with NAFLD or with other metabolic disorders. Keywords: fatty liver disease; cardiovascular disease; risk factors; anthropometry; nutrition; metabolic syndrome.
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http://dx.doi.org/10.21164/pomjlifesci.141DOI Listing
May 2018

Metabolites of arachidonic acid and linoleic acid in early stages of non-alcoholic fatty liver disease--A pilot study.

Prostaglandins Other Lipid Mediat 2015 Sep 25;121(Pt B):184-9. Epub 2015 Sep 25.

Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland. Electronic address:

Background: Nonalcoholic fatty liver disease (NAFLD) is a spectrum of liver conditions related to fat infiltration. The role of liver triacylglycerol accumulation in NAFLD is not fully understood.

Methods: Twenty-four patients, 12 in the first and 12 in the second stage of NAFLD, were prospectively enrolled in this study. Biochemical parameters and eicosanoids (HETE and HODE) were compared between the first and the second stage of hepatic steatosis and the effect of a 6-month dietary intervention on these parameters was evaluated. Eicosanoid profiles were extracted from 0.5 ml of plasma using solid-phase extraction RP-18 SPE columns. The HPLC separations were performed on a 1260 liquid chromatograph.

Results: Patients with stage I NAFLD had a significantly higher level of HDL cholesterol and a lower level of 5-HETE. Patients with grade II steatosis had higher concentrations of 9-HODE. Following the six-month dietary intervention, hepatic steatosis resolved completely in all patients. This resulted in a significant decrease in the concentrations of all eicosanoids (LX4, 16-HETE, 13-HODE, 9-HODE, 15-HETE, 12-HETE, 5-oxoETE, 5-HETE) and key biochemical parameters (BMI, insulin, HOMA-IR, liver enzymes).

Conclusion: A significant reduction in the analyzed eicosanoids and a parallel reduction in fatty liver confirmed the usefulness of HETE and HODE in the assessment of NAFLD.
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http://dx.doi.org/10.1016/j.prostaglandins.2015.09.003DOI Listing
September 2015

Fatty acid changes help to better understand regression of nonalcoholic fatty liver disease.

World J Gastroenterol 2015 Jan;21(1):301-10

Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.

Aim: To investigate whether liver steatosis reduction due to a six-month dietary intervention results in significant changes in the concentrations of fatty acids.

Methods: A group of 35 Caucasian individuals diagnosed with different levels of steatosis were prospectively enrolled in the present study. Analysis of the fatty acid profiles was performed according to changes in liver steatosis (liver steatosis reduction by one or two degrees) after a six-month dietary intervention. The diet helped reduce body mass in obese and overweight patients, and stabilize both glycemia and dyslipidemia. Fatty acids were extracted according to the Folch method and analyzed by gas chromatography.

Results: This study showed significant changes in fatty acid profiles in patients who had reduced liver steatosis by one as well as two degrees. A reduction in liver steatosis by one degree caused a significant increase in the level of the n-3 family: eicosapentaenoic acid (P < 0.055), docosapentaenoic acid-C 22:5 (P < 0.05) and docosahexaenoic acid (P < 0.05). A reduction in liver steatosis by two degrees caused a significant decrease in serum palmitoleic acid-C 16:1 (P < 0.05).

Conclusion: Liver steatosis reduction is associated with changes in fatty acid profiles, and these changes may reflect an alteration in fatty acid synthesis and metabolism. These findings may help better understand regression of nonalcoholic fatty liver disease.
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http://dx.doi.org/10.3748/wjg.v21.i1.301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284349PMC
January 2015

Usefulness of marking alkaline phosphatase and C-reactive protein in monitoring the risk of preterm delivery.

Med Sci Monit 2011 Nov;17(11):CR657-662

Department of Obstetrics and Perinatology Jagiellonian University, Cracow, Poland.

Background: The purpose of this paper is to compare the effectiveness of use of alkaline phosphatase (ALP) and C-reactive protein (CRP) levels in marking and monitoring the risk of preterm delivery due to infection.

Material/methods: The study involved 83 patients assigned to groups: Group I (n=43) consisted of patients hospitalized for symptoms of preterm delivery, and Group II (control group n=40) consisted of patients controlled or hospitalized delivering on time without complications, whose pregnancy had a physiological course. All patients had a single marking of ALP and CRP levels in serum performed.

Results: CRP levels were within the range 7 mg/l to 94 mg/l in the study group, and 4.83 mg/l to 90 mg/l in the control group. The level of ALP in the study group ranged from 139 u/l to 368 u/l and from 218 u/l to 321 u/l in the control group. In more than half of women (72.1%) from study group, CRP level exceeded 7 mg/l; in the control group, the CRP level exceeded 7 mg/l in 35% of cases. Significantly higher levels of CRP (above 20 mg/l) and ALP (above 300 u/l) were found in the 18 patients from the study group compared to the control group.

Conclusions: Although an increase in the level of ALP in serum cannot be an absolute and sole marker of the risk of preterm delivery, it can be used in conjunction with a significantly elevated CRP level.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539487PMC
http://dx.doi.org/10.12659/msm.882052DOI Listing
November 2011

Doppler velocimetry of the materno-fetal circulation in preterm delivered pregnancies complicated with hypertension.

Neuro Endocrinol Lett 2009 ;30(3):403-8

Department of Obstetrics and Perinatology, Jagielloniam University, Cracow, Poland.

Background: Hypertension is one of the most frequent complications of pregnancy. Due to high risk of morbidity and mortality in both mothers and children, it is necessary to continuously monitor the pregnancy, principally with biophysical methods. Particularly, doppler velocimetry of the materno-fetal circulation proves useful. THE AIM of the study was to assess the usefulness of doppler test in monitoring the condition of the foetus in preterm delivered pregnancy complicated with hypertension.

Material And Methods: The retrospective analysis comprised the data of 116 women who delivered prematurely at the Clinics of the Department of Gynaecology and Obstetrics at the Collegium Medicum of the Jagiellonian University in the years 2006-2007, resulting in creation of Group I involving 38 pregnant women with preeclampsia, and Group II of 36 women whose pregnancy was complicated with gestational hypertension. Control group was formed of 42 women with correct arterial blood pressure. When describing the groups, the differences in the birth weight and Apgar score were indicated.

Results: A significant statistical difference was found in the area of pulsation rate in the umbilical artery and cerebro-placental ratio (CPR). In the case of preterm delivery complicated with arterial pressure disorders, the foetus is characterised with worse organic perfusion and slower somatic growth than if no concomitant hypertension is present. Hypertension forms an additional risk factor in the course of preterm delivery, and doppler velocimetry is a good method for monitoring the condition of the foetus, as it allows for detection of irregularities and for implementation of relevant treatment to improve the newborn's condition at birth.
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February 2010

[Surgical strategy and outcome in multiple cerebral aneurysms].

Przegl Lek 2004 ;61(5):477-81

Klinika Neurochirurgii Collegium Medicum, Uniwersytetu Jagiellońskeigo, Kraków.

Introduction: Multiple aneurysms of the brain are a more dangerous disorder than single aneurysms. Most of patients have evidence of aneurysmal rupture, but rebleeding remains an additional danger because of co-existing aneurysms. These "silent" aneurysms also have to be operated--the risk of following subarachnoid haemorrhage (SAH) is about 2% annually.

Aim: The purpose of this study was to assess of the surgical outcome in multiple aneurysmal SAH in regard to chosen risk factors.

Material And Method: Retrospective analysis of 608 patients treated within three years (2001-2003) for cerebral aneurysms in the Department of Neurosurgery, Jagiellonian University in Kraków, was carried out. 14% patients had multiple aneurysms: 72--two aneurysms, 10 patients three aneurysms, and three patients four and more. The mean age was 50, with slight predominance of women (58%). Most patients were operated on via pterional craniotomy, and posterior circulation aneurysms were clipped by means of suboccipital approach. "Silent" aneurysms being clipped in the first stage, if were available via the same craniotomy, another--in the next stage. We analyzed short-term outcome of multiple aneurysms in comparison to the single (literature data). We presented the outcome (according to the Glasgow Outcome Scale) with regard to preoperative condition, age, location of the most frequent "bleeding" aneurysms (middle cerebral artery, anterior communicating artery), and in patients operated on in the 1st and 2nd stage. Accuracy of the preoperative detection of "bleeding" aneurysm was checked based on CT, MRI, angio-CT, angio-MRI and DSA.

Results And Conclusions: Out of all 85 treated patients, 64 (75.3%) presented very good and good condition on discharge. Five (5.9%) were severe disabled, and 16 (18.8%) died. Good preoperative condition, age less than 55, and "bleeding" aneurysms of the middle cerebral artery (compared with anterior communicating artery) are good prognostic factors in surgically treated multiple aneurysms. Outcome in single and multiple aneurysms is similar, excluding perioperative losses, which are 5% higher in the multiple aneurysms group. In our series in each case of "bleeding" aneurysm was correctly detected preoperatively.
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February 2005