Publications by authors named "Pingping Wang"

163 Publications

Regulation of Plant Responses to Salt Stress.

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan 250014, China.

Salt stress is a major environmental stress that affects plant growth and development. Plants are sessile and thus have to develop suitable mechanisms to adapt to high-salt environments. Salt stress increases the intracellular osmotic pressure and can cause the accumulation of sodium to toxic levels. Thus, in response to salt stress signals, plants adapt via various mechanisms, including regulating ion homeostasis, activating the osmotic stress pathway, mediating plant hormone signaling, and regulating cytoskeleton dynamics and the cell wall composition. Unraveling the mechanisms underlying these physiological and biochemical responses to salt stress could provide valuable strategies to improve agricultural crop yields. In this review, we summarize recent developments in our understanding of the regulation of plant salt stress.
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http://dx.doi.org/10.3390/ijms22094609DOI Listing
April 2021

Structure and Properties of Octenyl Succinic Anhydride-Modified High-Amylose Japonica Rice Starches.

Polymers (Basel) 2021 Apr 18;13(8). Epub 2021 Apr 18.

School of Liquor and Food Engineering, Guizhou University, Guiyang 550000, China.

Starches rich in amylose are promising functional ingredients for calory-reduced foods. In this research, a high-amylose Japonica rice starch (amylose content 33.3%) was esterified with octenyl succinic anhydride (OSA) to improve the functional properties. The OSA-modified derivatives were evaluated for structure and functional properties, with OSA-modified normal Japonica rice starch (amylose content 18.8%) used as control. Fourier transform infrared spectra confirmed the introduction of OSA groups to starch. OSA modification made little change to morphology and particle size of high-amylose starch, but decreased the relative crystallinity and pasting temperature and increased the pasting viscosity, swelling power, emulsifying stability, and resistant starch (RS) content. The changes of properties were related to the degree of substitution (DS). Typically, OSA-modified high-amylose starch at DS of 0.0285 shows polyhedral-shape granules, with a volume-average particle diameter of 8.87 μm, peak viscosity of 5730 cp, and RS content of 35.45%. OSA-modified high-amylose starch had greater peak viscosity and RS content and lower swelling power than OSA-modified normal starch of similar DS, but the two kinds of derivatives did not have a significant difference in emulsifying stability. The OSA-modified high-amylose Japonica rice starch could be used as an emulsifier, thickener, and fat replacer in food systems.
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http://dx.doi.org/10.3390/polym13081325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073360PMC
April 2021

4-phenylbutyrate exerts stage-specific effects on cardiac differentiation via HDAC inhibition.

PLoS One 2021 21;16(4):e0250267. Epub 2021 Apr 21.

School of Medicine, Hunan Normal University, Changsha, Hunan, China.

4-phenylbutyrate (4-PBA), a terminal aromatic substituted fatty acid, is used widely to specifically attenuate endoplasmic reticulum (ER) stress and inhibit histone deacetylases (HDACs). In this study, we investigated the effect of 4-PBA on cardiac differentiation of mouse embryonic stem (ES) cells. Herein, we found that 4-PBA regulated cardiac differentiation in a stage-specific manner just like trichostatin A (TSA), a well-known HDAC inhibitor. 4-PBA and TSA favored the early-stage differentiation, but inhibited the late-stage cardiac differentiation via acetylation. Mechanistic studies suggested that HDACs exhibited a temporal expression profiling during cardiomyogenesis. Hdac1 expression underwent a decrease at the early stage, while was upregulated at the late stage of cardiac induction. During the early stage of cardiac differentiation, acetylation favored the induction of Isl1 and Nkx2.5, two transcription factors of cardiac progenitors. During the late stage, histone acetylation induced by 4-PBA or TSA interrupted the gene silence of Oct4, a key determinant of self-renewal and pluripotency. Thereby, 4-PBA and TSA at the late stage hindered the exit from pluripotency, and attenuated the expression of cardiac-specific contractile proteins. Overexpression of HDAC1 and p300 exerted different effects at the distinct stages of cardiac induction. Collectively, our study shows that timely manipulation of HDACs exhibits distinct effects on cardiac differentiation. And the context-dependent effects of HDAC inhibitors depend on cell differentiation states marked by the temporal expression of pluripotency-associated genes.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250267PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059837PMC
April 2021

Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K.

Synth Syst Biotechnol 2021 Jun 31;6(2):69-76. Epub 2021 Mar 31.

CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai, 200032, China.

Ginsenoside Compound K (CK) has been recognized as a major functional component that is absorbed into the systemic circulation after oral administration of ginseng. CK demonstrates diverse bioactivities. A phase I clinical study indicated that CK was a potential candidate for arthritis therapy. However, a phase II clinical study was suspended because of the high cost associated with the present CK manufacturing approach, which is based on the traditional planting-extracting-biotransforming process. We previously elucidated the complete CK biosynthetic pathway and realized for the first time biosynthesis of CK from glucose by engineered yeast. However, CK production was not sufficient for industrial application. Here, we systematically engineered to achieve high titer production of CK from glucose using a previously constructed protopanaxadiol (PPD)-producing chassis, optimizing UGTPg1 expression, improving UDP-glucose biosynthesis, and tuning down UDP-glucose consumption. Our final engineered yeast strain produced CK with a titer of 5.74 g/L in fed-batch fermentation, which represents the highest CK production in microbes reported to date. Once scaled-up, this high titer microbial biosynthesis platform will enable a robust and stable supply of CK, thus facilitating study and medical application of CK.
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http://dx.doi.org/10.1016/j.synbio.2021.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040117PMC
June 2021

High-level sustainable production of the characteristic protopanaxatriol-type saponins from Panax species in engineered Saccharomyces cerevisiae.

Metab Eng 2021 Apr 15;66:87-97. Epub 2021 Apr 15.

CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. Electronic address:

The Chinese medicinal plant Panax notoginseng has been traditionally used to activate blood flow and circulation, and to prevent blood stasis. P. notoginseng contains protopanaxatriol (PPT)-type saponins as its main active compounds, thus distinguishing it from the other two famous Panax species, P. ginseng and P. quinquefolius. Ginsenoside Rg1 (Rg1), notoginsenoside R1 (NgR1), and notoginsenoside R2 (NgR2) are three major PPT-type saponins in P. notoginseng and possess potential cardiovascular protection activities. However, their use in medical applications has long been hampered by the lack of sustainable and low-cost industrial-scale preparation methods. In this study, a PPT-producing yeast chassis strain was designed and constructed based on a previously constructed and optimized protopanaxadiol (PPD)-producing Saccharomyces cerevisiae strain, and further optimized by systemically engineering and optimizing the expression level of its key P450 biopart. Rg1-producing yeast strains were constructed by introducing PgUGT71A53 and PgUGT71A54 into the PPT chassis strain. The fermentation titer of Rg1 reached 1.95 g/L. A group of UDP-glycosyltransferases (UGT) from P. notoginseng and P. ginseng were characterized, and were found to generate NgR1 and NgR2 by catalyzing the C-O-Glc xylosylation of Rg1 and Rh1, respectively. Using one of these UGTs, PgUGT94Q13, and the previously identified PgUGT71A53 and PgUGT71A54, the biosynthetic pathway to produce saponins NgR1 and NgR2 from PPT could be available. The NgR1 cell factory was further developed by introducing PgUGT94Q13 and a heterologous UDP-xylose biosynthetic pathway from Arabidopsis thaliana into the highest Rg1-producing cell factory. The NgR2-producing cell factory was constructed by introducing PgUGT71A54, PgUGT94Q13, and the UDP-xylose biosynthetic pathway into the PPT chassis. De novo production of NgR1 and NgR2 reached 1.62 g/L and 1.25 g/L, respectively. Beyond the realization of artificial production of the three valuable saponins Rg1, NgR1, and NgR2 from glucose, our work provides a green and sustainable platform for the efficient production of other PPT-type saponins in engineered yeast strains, and promotes the industrial application of PPT-type saponins as medicine and functional foods.
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http://dx.doi.org/10.1016/j.ymben.2021.04.006DOI Listing
April 2021

Arbuscular mycorrhizal fungi improve growth, essential oil, secondary metabolism, and yield of tobacco (Nicotiana tabacum L.) under drought stress conditions.

Environ Sci Pollut Res Int 2021 Apr 16. Epub 2021 Apr 16.

College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Drought is a major environmental threat limiting worldwide crop production. Drought stress affects the tobacco quality and yield; therefore, the current research studies were undertaken to investigate the effectiveness of arbuscular mycorrhizal fungi (AMF) under drought stress on morphological and biochemical attributes of tobacco (Nicotiana tabacum L. variety Yunyan 87). AMF-inoculated and AMF-non-inoculated plants were maintained in a greenhouse and irrigated with a half-strength Hoagland solution (100 mL pot) once a week. At harvesting, the plant height, number of leaves, fresh and dry weights, mycorrhizal colonization, and concentration of leaf photosynthetic pigments and photosynthetic rate were measured. Data were statistically analyzed by ANOVA and the principal component (PCA) analyses. The effect of root colonization significantly increased biomass production and essential oil accumulation. Results showed that drought at mild and severe stressed levels significantly affected tobacco growth by decreasing plant height, biomass, and a number of leaves. However, inoculation of AMF considerably increased plant height, fresh and dry weights, chlorophyll (a, b), total chlorophyll, and carotenoid content by 43.84, 40.87 and 49.76, 185.29, 325.60, 173.12, and 211.49%, respectively. Compared with non-inoculated plants, AMF inoculation significantly enhanced the essential oil yield and the uptake of nitrogen, phosphorus, and potassium with the increase of 257.36, 102.71, and 90.76, 62.32, and 84.51%, respectively, in mild drought + AMF-treated plants. Similarly, the antioxidant enzymatic activity, glomalin-related soil protein (GRSP), and accumulation of phenols and flavonoids and osmolytes content were also significantly improved in inoculated plants under drought stress. Additionally, AMF inoculation significantly upregulated the lipoxygenase (LOX) and phenylalanine ammonia-lyase (PAL) enzymes by 197 and 298.44% under drought conditions. These findings depicted that the symbiotic association of AMF improved the overall growth pattern and secondary metabolism in tobacco plants under severe drought stress conditions and may be used as an approaching source of important drugs in the field of pharmacology.
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http://dx.doi.org/10.1007/s11356-021-13755-3DOI Listing
April 2021

Asymmetric Cell Division of Fibroblasts is An Early Deterministic Step to Generate Elite Cells during Cell Reprogramming.

Adv Sci (Weinh) 2021 04 25;8(7):2003516. Epub 2021 Feb 25.

Department of Bioengineering University of California Los Angeles Los Angeles CA 90095 USA.

Cell reprogramming is considered a stochastic process, and it is not clear which cells are prone to be reprogrammed and whether a deterministic step exists. Here, asymmetric cell division (ACD) at the early stage of induced neuronal (iN) reprogramming is shown to play a deterministic role in generating elite cells for reprogramming. Within one day, fibroblasts underwent ACD, with one daughter cell being converted into an iN precursor and the other one remaining as a fibroblast. Inhibition of ACD significantly inhibited iN conversion. Moreover, the daughter cells showed asymmetric DNA segregation and histone marks during cytokinesis, and the cells inheriting newly replicated DNA strands during ACD became iN precursors. These results unravel a deterministic step at the early phase of cell reprogramming and demonstrate a novel role of ACD in cell phenotype change. This work also supports a novel hypothesis that daughter cells with newly replicated DNA strands are elite cells for reprogramming, which remains to be tested in various reprogramming processes.
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http://dx.doi.org/10.1002/advs.202003516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025021PMC
April 2021

Pulmonary infiltration as the initial manifestation of chronic lymphoproliferative disorder of natural killer cells: a case report and literature review.

BMC Pulm Med 2021 Mar 19;21(1):94. Epub 2021 Mar 19.

Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang, 110001, Liaoning, China.

Background: Chronic lymphoproliferative disorder of natural killer cells (CLPD-NK) is an extremely rare haematological disease. To the best of our knowledge, pulmonary infiltration in CLPD-NK has not been reported before. Our case study aimed to present the clinical characteristics, chest computed tomography (CT) findings, and flow cytometry immunophenotyping (FCI) results of an unusual case of migratory pulmonary infiltration in a patient with CLPD-NK.

Case Presentation: A 51-year-old female patient was admitted to our hospital on October 8, 2019. Eight months before this visit, she had been diagnosed with pneumonia in a community hospital with 1 month of low-grade fever and had recovered after oral antibiotic administration. During follow-up, the patient presented with persistent peripheral blood (PB) lymphocytosis and ground-glass opacities on lung CT scans without any symptoms and signs or any evidence of infectious, allergic or autoimmunity pulmonary diseases. Abnormal NK cells were identified in the PB, bone marrow and bronchoalveolar lavage fluid (BALF) using FCI in our hospital. Eventually, the patient was diagnosed with pulmonary infiltration of CLPD-NK. The patient had an indolent clinical course without symptoms, hepatosplenomegaly or palpable lymphadenopathy and did not receive any therapy. The patient has remained in a good performance status 13 months after the diagnosis.

Conclusions: Our study described a unique case of pulmonary infiltration in a patient with CLPD-NK. The present case highlights the importance of FCI of the BALF in patients with lymphocytosis and pulmonary shadows to avoid misdiagnosis.
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http://dx.doi.org/10.1186/s12890-021-01457-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977295PMC
March 2021

Skeletal muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro.

Nat Biomed Eng 2021 Mar 18. Epub 2021 Mar 18.

Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, USA.

Muscle loss and impairment resulting from traumatic injury can be alleviated by therapies using muscle stem cells. However, collecting sufficient numbers of autologous myogenic stem cells and expanding them efficiently has been challenging. Here we show that myogenic stem cells (predominantly Pax7 cells)-which were selectively expanded from readily obtainable dermal fibroblasts or skeletal muscle stem cells using a specific cocktail of small molecules and transplanted into muscle injuries in adult, aged or dystrophic mice-led to functional muscle regeneration in the three animal models. We also show that sustained release of the small-molecule cocktail in situ through polymer nanoparticles led to muscle repair by inducing robust activation and expansion of resident satellite cells. Chemically induced stem cell expansion in vitro and in situ may prove to be advantageous for stem cell therapies that aim to regenerate skeletal muscle and other tissues.
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http://dx.doi.org/10.1038/s41551-021-00696-yDOI Listing
March 2021

COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas.

Cell 2021 04 3;184(7):1895-1913.e19. Epub 2021 Feb 3.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China.

A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes are associated with distinct clinical features, including age, sex, severity, and disease stages of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within virus-positive cells. Systemic upregulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis of and developing effective therapeutic strategies for COVID-19.
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http://dx.doi.org/10.1016/j.cell.2021.01.053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857060PMC
April 2021

Prediction of Lymph-Node Metastasis in Cancers Using Differentially Expressed mRNA and Non-coding RNA Signatures.

Front Cell Dev Biol 2021 11;9:605977. Epub 2021 Feb 11.

College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, China.

Accurate prediction of lymph-node metastasis in cancers is pivotal for the next targeted clinical interventions that allow favorable prognosis for patients. Different molecular profiles (mRNA and non-coding RNAs) have been widely used to establish classifiers for cancer prediction (e.g., tumor origin, cancerous or non-cancerous state, cancer subtype). However, few studies focus on lymphatic metastasis evaluation using these profiles, and the performance of classifiers based on different profiles has also not been compared. Here, differentially expressed mRNAs, miRNAs, and lncRNAs between lymph-node metastatic and non-metastatic groups were identified as molecular signatures to construct classifiers for lymphatic metastasis prediction in different cancers. With this similar feature selection strategy, support vector machine (SVM) classifiers based on different profiles were systematically compared in their prediction performance. For representative cancers (a total of nine types), these classifiers achieved comparative overall accuracies of 81.00% (67.96-92.19%), 81.97% (70.83-95.24%), and 80.78% (69.61-90.00%) on independent mRNA, miRNA, and lncRNA datasets, with a small set of biomarkers (6, 12, and 4 on average). Therefore, our proposed feature selection strategies are economical and efficient to identify biomarkers that aid in developing competitive classifiers for predicting lymph-node metastasis in cancers. A user-friendly webserver was also deployed to help researchers in metastasis risk determination by submitting their expression profiles of different origins.
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http://dx.doi.org/10.3389/fcell.2021.605977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905047PMC
February 2021

prPred: A Predictor to Identify Plant Resistance Proteins by Incorporating k-Spaced Amino Acid (Group) Pairs.

Front Bioeng Biotechnol 2020 21;8:645520. Epub 2021 Jan 21.

School of Electronic and Communication Engineering, Shenzhen Polytechnic, Shenzhen, China.

To infect plants successfully, pathogens adopt various strategies to overcome their physical and chemical barriers and interfere with the plant immune system. Plants deploy a large number of resistance (R) proteins to detect invading pathogens. The R proteins are encoded by resistance genes that contain cell surface-localized receptors and intracellular receptors. In this study, a new plant R protein predictor called prPred was developed based on a support vector machine (SVM), which can accurately distinguish plant R proteins from other proteins. Experimental results showed that the accuracy, precision, sensitivity, specificity, F1-score, MCC, and AUC of prPred were 0.935, 1.000, 0.806, 1.000, 0.893, 0.857, and 0.948, respectively, on an independent test set. Moreover, the predictor integrated the HMMscan search tool and Phobius to identify protein domain families and transmembrane protein regions to differentiate subclasses of R proteins. prPred is available at https://github.com/Wangys-prog/prPred. The tool requires a valid Python installation and is run from the command line.
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http://dx.doi.org/10.3389/fbioe.2020.645520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859348PMC
January 2021

Systematic calibration method based on acceleration and angular rate measurements for fiber-optic gyro SINS.

Rev Sci Instrum 2021 Jan;92(1):015001

No.203 Research Institute of China Ordnance Industries, Xian 710065, China.

A simple systematic calibration method based on acceleration and angular rate measurements is introduced for the fiber-optic gyro strapdown inertial navigation system in this paper. Meanwhile, a unified mathematical framework and an iterative calculation method are designed for the systematic calibration method. Using this method, one can estimate the fiber-optic gyro inertial measurement unit (FOG IMU) parameters both at a manufacturer's facility and in the field. In order to get all FOG IMU parameters, a procedure adopted based on this approach consists of two stages: First, FOG IMU raw data (accelerometer and gyro readouts) are accumulated in 19 specified FOG IMU positions. Second, the accumulated data are processed by special software to estimate all FOG IMU parameters. In addition, observability analysis of the method in 19 specified FOG IMU positions is done without the limitation of FOG IMU's initial orientation, and this analysis provides theoretical support for the application in a complex terrain. Moreover, the influence of gravity disturbance is analyzed for the first time. The analysis and experiment results show that the systematic calibration method provided by this work can meet the requirement of FOG IMU calibration.
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http://dx.doi.org/10.1063/5.0023674DOI Listing
January 2021

Comprehensive analysis of TCR repertoire in COVID-19 using single cell sequencing.

Genomics 2021 03 28;113(2):456-462. Epub 2020 Dec 28.

School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China; Key Laboratory of Biological Big Data (Harbin Institute of Technology), Ministry of Education, China. Electronic address:

T-cell receptor (TCR) is crucial in T cell-mediated virus clearance. To date, TCR bias has been observed in various diseases. However, studies on the TCR repertoire of COVID-19 patients are lacking. Here, we used single-cell V(D)J sequencing to conduct comparative analyses of TCR repertoire between 12 COVID-19 patients and 6 healthy controls, as well as other virus-infected samples. We observed distinct T cell clonal expansion in COVID-19. Further analysis of VJ gene combination revealed 6 VJ pairs significantly increased, while 139 pairs significantly decreased in COVID-19 patients. When considering the VJ combination of α and β chains at the same time, the combination with the highest frequency on COVID-19 was TRAV12-2-J27-TRBV7-9-J2-3. Besides, preferential usage of V and J gene segments was also observed in samples infected by different viruses. Our study provides novel insights on TCR in COVID-19, which contribute to our understanding of the immune response induced by SARS-CoV-2.
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http://dx.doi.org/10.1016/j.ygeno.2020.12.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833309PMC
March 2021

Identification of Sub-Golgi protein localization by use of deep representation learning features.

Bioinformatics 2020 Dec 26. Epub 2020 Dec 26.

Center for Bioinformatics, School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150000, China.

Motivation: The Golgi apparatus has a key functional role in protein biosynthesis within the eukaryotic cell with malfunction resulting in various neurodegenerative diseases. For a better understanding of the Golgi apparatus, it is essential to identification of sub-Golgi protein localization. Although some machine learning methods have been used to identify sub-Golgi localization proteins by sequence representation fusion, more accurate sub-Golgi protein identification is still challenging by existing methodology.

Results: we developed a protein sub-Golgi localization identification protocol using deep representation learning features with 107 dimensions. By this protocol, we demonstrated that instead of multi-type protein sequence feature representation fusion as in previous state-of-the-art sub-Golgi-protein localization classifiers, it is sufficient to exploit only one type of feature representation for more accurately identification of sub-Golgi proteins. Compared with independent testing results for benchmark datasets, our protocol is able to perform generally, reliably, and robustly for sub-Golgi protein localization prediction.

Availability: A use-friendly webserver is freely accessible at http://isGP-DRLF.aibiochem.net and the prediction code is accessible at https://github.com/zhibinlv/isGP-DRLF.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btaa1074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023683PMC
December 2020

Naoxueshu relieves hematoma after clot removal in acute spontaneous intracerebral hemorrhage.

Brain Behav 2021 Jan 4;11(1):e01957. Epub 2020 Dec 4.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objectives: Surgical treatment is expected to remove clot immediately in acute spontaneous intracerebral hemorrhage (SICH) patients. The aim of this study was to evaluate whether Naoxueshu could enhance the efficacy of clot removal surgery in acute SICH patients.

Methods: One hundred twenty patients who had been diagnosed as SICH according to neuroimaging were enrolled in this study. They received craniotomy, decompressive craniectomy, or minimally invasive surgical evacuation as appropriate and then were randomized into two groups: the Naoxueshu group (NXS group, n = 60) and the control group (n = 60). All the patients received standard medical management while patients in NXS group also took Naoxueshu oral liquid 10 ml with three times a day for seven consecutive days. The primary outcome was the 7-day hematoma volume and secondary outcomes were 7-day National Institutes of Health Stroke Scale (NIHSS) score and 7-day cerebral edema score.

Results: After clot removal surgery, hematoma volume in NXS group (9.5 ± 8.0) was significantly decreased than that in Control group (21.3 ± 22.9, p < .0001) 7 days after surgery. Moreover, cerebral edema was also relieved after 7-day's Naoxueshu treatment (2.5 ± 0.9 vs. 2.9 ± 0.7, p = .043). Since patients in NXS group had worse baseline NIHSS score (17.2 ± 8.1 vs. 13.7 ± 10.1, p = .039), it was reasonable to conclude that Naoxueshu treatment could improve patients' neurological function because 7-day NIHSS score of the two groups was similar.

Conclusion: Naoxueshu oral liquid could relieve hematoma volume and cerebral edema after clot removal surgery in acute SICH patients. Moreover, it had the potential to improve patients' short-term neurological function.
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http://dx.doi.org/10.1002/brb3.1957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821564PMC
January 2021

Discrimination of Thermophilic Proteins and Non-thermophilic Proteins Using Feature Dimension Reduction.

Front Bioeng Biotechnol 2020 22;8:584807. Epub 2020 Oct 22.

Information and Computer Engineering College, Northeast Forestry University, Harbin, China.

Thermophilicity is a very important property of proteins, as it sometimes determines denaturation and cell death. Thus, methods for predicting thermophilic proteins and non-thermophilic proteins are of interest and can contribute to the design and engineering of proteins. In this article, we describe the use of feature dimension reduction technology and LIBSVM to identify thermophilic proteins. The highest accuracy obtained by cross-validation was 96.02% with 119 parameters. When using only 16 features, we obtained an accuracy of 93.33%. We discuss the importance of the different characteristics in identification and report a comparison of the performance of support vector machine to that of other methods.
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http://dx.doi.org/10.3389/fbioe.2020.584807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642589PMC
October 2020

Correlation between obesity and clinicopathological characteristics in patients with papillary thyroid cancer: a study of 1579 cases: a retrospective study.

PeerJ 2020 8;8:e9675. Epub 2020 Sep 8.

Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Objective: To explore the relationship between body mass index (BMI) and clinicopathological characteristics in patients with papillary thyroid carcinoma (PTC).

Methods: The clinical data of 1,579 patients with PTC, admitted to our hospital from May 2016 to March 2017, were retrospectively analyzed. According to the different BMI of patients, it can be divided into underweight recombination (BMI < 18.5 kg/m), normal body recombination (18.5 ≤ BMI < 24.0 kg/m), overweight recombination (24.0 ≤ BMI < 28.0 kg/m) and obesity group (BMI ≥ 28.0 kg/m). The clinicopathological characteristics of PTC in patients with different BMIs group were compared.

Results: In our study, the risk for extrathyroidal extension (ETE), advanced T stage (T III/IV), and advanced tumor-node-metastasis stage (TNM III/IV) in the overweight group were higher, with OR (odds ratio) = 1.99(1.41-2.81), OR = 2.01(1.43-2.84), OR = 2.94(1.42-6.07), respectively, relative to the normal weight group. The risk for ETE and T III/IV stage in the obese group were higher, with OR = 1.82(1.23-2.71) and OR = 1.82(1.23-2.70), respectively, relative to the normal weight group.

Conclusion: BMI is associated with the invasiveness of PTC. There is a higher risk for ETE and TNM III/IV stage among patients with PTC in the overweight group and for ETE among patients with PTC in the obese group.
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http://dx.doi.org/10.7717/peerj.9675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485482PMC
September 2020

LncRNA TUG1 Contributes to Hypoxia-Induced Myocardial Cell Injury Through Downregulating miR-29a-3p in AC16 Cells.

J Cardiovasc Pharmacol 2020 11;76(5):533-539

Department of Cardiology, Lanling County People's Hospital, Linyi, Shandong, China.

Myocardial ischemia is a common reason that causes human death globally. Long noncoding RNA taurine upregulated 1 (TUG1) serves as an oncogene in a variety of cancers. In this article, we aimed to investigate the role of TUG1 and its underlying signal pathway in hypoxia-induced myocardial cell injury. Cell viability, apoptosis, and lactate dehydrogenase (LDH) release were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry, western blot assay, and LDH cytotoxicity assay. Quantitative real-time polymerase chain reaction was applied to measure the enrichment of TUG1 and miR-29a-3p. MiR-29a-3p was predicted as a target of TUG1 by StarBase bioinformatic software, and the target relationship between TUG1 and miR-29a-3p was verified by dual-luciferase reporter assay. Hypoxia treatment induced the apoptosis and LDH release while inhibited the viability of AC16 cells. TUG1 was markedly upregulated while the level of miR-29a-3p was notably decreased in hypoxia-stimulated AC16 cells. TUG1 contributed to hypoxia-induced AC16 injury. MiR-29a-3p depletion intensified hypoxia-induced AC16 damage. TUG1 negatively regulated the expression of miR-29a-3p through their direct interaction in AC16 cells. TUG1 silencing-mediated influences in hypoxia-induced AC16 cells were partly reversed by the interference of miR-29a-3p. In conclusion, TUG1 accelerated hypoxia-induced AC16 injury through inversely modulating the level of miR-29a-3p. TUG1/miR-29a-3p axis might be an underlying therapeutic target for myocardial ischemia.
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http://dx.doi.org/10.1097/FJC.0000000000000906DOI Listing
November 2020

Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats.

Biomed Res Int 2020 21;2020:3049302. Epub 2020 Oct 21.

Department of Critical Care Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, China.

The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-, IL-1, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-, IL-1, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.
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http://dx.doi.org/10.1155/2020/3049302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596461PMC
May 2021

Epidemiological and phylogenetic analysis of mumps virus isolated from 2016 to 2019 in Henan Province, China.

Jpn J Infect Dis 2020 Oct 30. Epub 2020 Oct 30.

The Sixth People's Hospital of Zhengzhou, China.

Routine vaccination has proven to be highly effective in reducing the incidence of mumps. However, sporadic cases and/or mumps outbreaks do occur in children and adolescents younger than 15 years old, particularly among those 5-9 years old. To explore the characteristics of such outbreaks in Henan Province, clinical data of patients infected with MuV were collected, and the isolated MuV strains were phylogenetically analyzed. Of a total of 426 samples, MuV RNA targeting the small hydrophobic (SH) gene was detected in 153 samples. MuV-positive cases in each age group (< 5, 5-9,10-15, 16-19, and ≥ 20) accounted for 1%, 17%, 12%, 2% and 4% of the total number of cases, respectively. Phylogenetic analysis based on the SH gene sequences indicated that all of the isolated strains were of genotype F and isolates in the the same subcluster and identical SH gene sequences tended to be derived from the same community or municipalities when analyzed alongside epidemiological data. In conclusion, the incidence of mumps in Henan Province is high. The data provided in this study might promote further research in the clarification of specific cause of mumps outbreaks and which will facilitate the implementation of effective prevention and control measures.
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http://dx.doi.org/10.7883/yoken.JJID.2020.649DOI Listing
October 2020

Properties of flour from pearled wheat kernels as affected by ozone treatment.

Food Chem 2021 Mar 30;341(Pt 2):128203. Epub 2020 Sep 30.

Department of Grain Science and Industry, Kansas State University, Manhattan 66506, USA. Electronic address:

Two different pearling degrees of wheat kernels (lightly-pearled: 14.4% and heavily-pearled: 38.9%) and un-pearled kernels were treated with ozone and evaluated for flour compositions and properties. Ozonation did not change main compositions and damaged starch content of three kernels' flours. Flour brightness of all three kernels was improved after ozone treatment. Ozonation enhanced the dough strength of the flours from un-pearled and pearled kernels and the effect elevated with increasing pearling degree. Ozone treatment increased the peak viscosity of flour and the level of increase in heavily-pearled kernels was greater than un-pearled and lightly-pearled. Ozonation resulted in an increase in the insoluble protein polymer content of heavily-pearled kernels' flour, but only had a slight effect on un-pearled lightly-pearled kernels. After ozone treatment, un-pearled and lightly-pearled kernels exhibited increases in molecular weight of starch, but heavily-pearled resulted in the opposite trend.
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http://dx.doi.org/10.1016/j.foodchem.2020.128203DOI Listing
March 2021

Interplay between the Ubiquitin Proteasome System and Ubiquitin-Mediated Autophagy in Plants.

Cells 2020 10 1;9(10). Epub 2020 Oct 1.

Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan 250014, China.

All eukaryotes rely on the ubiquitin-proteasome system (UPS) and autophagy to control the abundance of key regulatory proteins and maintain a healthy intracellular environment. In the UPS, damaged or superfluous proteins are ubiquitinated and degraded in the proteasome, mediated by three types of ubiquitin enzymes: E1s (ubiquitin activating enzymes), E2s (ubiquitin conjugating enzymes), and E3s (ubiquitin protein ligases). Conversely, in autophagy, a vesicular autophagosome is formed that transfers damaged proteins and organelles to the vacuole, mediated by a series of ATGs (autophagy related genes). Despite the use of two completely different componential systems, the UPS and autophagy are closely interconnected and mutually regulated. During autophagy, ATG8 proteins, which are autophagosome markers, decorate the autophagosome membrane similarly to ubiquitination of damaged proteins. Ubiquitin is also involved in many selective autophagy processes and is thus a common factor of the UPS and autophagy. Additionally, the components of the UPS, such as the 26S proteasome, can be degraded via autophagy, and conversely, ATGs can be degraded by the UPS, indicating cross regulation between the two pathways. The UPS and autophagy cooperate and jointly regulate homeostasis of cellular components during plant development and stress response.
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http://dx.doi.org/10.3390/cells9102219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600366PMC
October 2020

Discovery of novel dehydroabietic acid derivatives as DNA/BSA binding and anticancer agents.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 15;246:118944. Epub 2020 Sep 15.

College of Science, Nanjing Forestry University, Nanjing 210037, China.

To explore the biological properties of rosin derivatives, two dehydroabietic acid derivatives N-(5-dehydroabietyl-1,3,4-thiadiazole)-yl-pyridine-2-carboxamide (DTPC) and di-N-(5-dehydroabietyl-1,3,4-thiadiazole)-yl-pyridine-2,6-carboxamide (DDTPC) with 1,3,4-thiadiazole, pyridine and amide moieties were designed and synthesized according to superposition principle of activity group. They interact with calf thymus DNA (CT DNA) via intercalation based on the results of circular dichroism (CD) and fluorescence spectroscopy, DNA denaturation and viscosity studies. Fluorescence and CD spectral experiments indicate that they might be transported and stored by protein like bovine serum albumin (BSA). MTT assay was further carried out to examine their cytotoxicity, they both showed selective cytotoxicity and DTPC exhibited better cytotoxicity. The antiproliferative effect of DTPC toward A431 cell line was stronger than that of clinically used cisplatin and oxaliplatin. In addition, the cytotoxicity of DTPC and DDTPC was closely related with their DNA binding ability.
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http://dx.doi.org/10.1016/j.saa.2020.118944DOI Listing
February 2021

The unprecedented diversity of UGT94-family UDP-glycosyltransferases in Panax plants and their contribution to ginsenoside biosynthesis.

Sci Rep 2020 09 21;10(1):15394. Epub 2020 Sep 21.

CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences/Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

More than 150 ginsenosides have been isolated and identified from Panax plants. Ginsenosides with different glycosylation degrees have demonstrated different chemical properties and bioactivity. In this study, we systematically cloned and characterized 46 UGT94 family UDP-glycosyltransferases (UGT94s) from a mixed Panax ginseng/callus cDNA sample with high amino acid identity. These UGT94s were found to catalyze sugar chain elongation at C3-O-Glc and/or C20-O-Glc of protopanaxadiol (PPD)-type, C20-O-Glc or C6-O-Glc of protopanaxatriol (PPT)-type or both C3-O-Glc of PPD-type and C6-O-Glc of PPT-type or C20-O-Glc of PPD-type and PPT-type ginsenosides with different efficiencies. We also cloned 26 and 51 UGT94s from individual P. ginseng and P. notoginseng plants, respectively; our characterization results suggest that there is a group of UGT94s with high amino acid identity but diverse functions or catalyzing activities even within individual plants. These UGT94s were classified into three clades of the phylogenetic tree and consistent with their catalytic function. Based on these UGT94s, we elucidated the biosynthetic pathway of a group of ginsenosides. Our present results reveal a series of UGTs involved in second sugar chain elongation of saponins in Panax plants, and provide a scientific basis for understanding the diverse evolution mechanisms of UGT94s among plants.
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http://dx.doi.org/10.1038/s41598-020-72278-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506552PMC
September 2020

Visceral Mechano-sensing Neurons Control Drosophila Feeding by Using Piezo as a Sensor.

Neuron 2020 11 9;108(4):640-650.e4. Epub 2020 Sep 9.

School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing, 100084, China. Electronic address:

Animal feeding is controlled by external sensory cues and internal metabolic states. Does it also depend on enteric neurons that sense mechanical cues to signal fullness of the digestive tract? Here, we identify a group of piezo-expressing neurons innervating the Drosophila crop (the fly equivalent of the stomach) that monitor crop volume to avoid food overconsumption. These neurons reside in the pars intercerebralis (PI), a neuro-secretory center in the brain involved in homeostatic control, and express insulin-like peptides with well-established roles in regulating food intake and metabolism. Piezo knockdown in these neurons of wild-type flies phenocopies the food overconsumption phenotype of piezo-null mutant flies. Conversely, expression of either fly Piezo or mammalian Piezo1 in these neurons of piezo-null mutants suppresses the overconsumption phenotype. Importantly, Piezo neurons at the PI are activated directly by crop distension, thus conveying a rapid satiety signal along the "brain-gut axis" to control feeding.
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http://dx.doi.org/10.1016/j.neuron.2020.08.017DOI Listing
November 2020

Diagnostic Value of Contrast-Enhanced Ultrasound for Differential Diagnosis of Malignant and Benign Soft Tissue Masses: A Meta-Analysis.

Ultrasound Med Biol 2020 Dec 6;46(12):3179-3187. Epub 2020 Sep 6.

Department of Ultrasound, First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

This meta-analysis was aimed at investigating the value of using contrast-enhanced ultrasound (CEUS) in the differential diagnosis of benign and malignant soft tissue masses (STMs). Relevant studies published before March 24, 2020 were identified through a comprehensive search of PubMed, Ovid, Cochrane and Web of Science. According to the inclusion criteria, five studies were selected comprising 746 patients. In the differential diagnosis of benign and malignant STMs, the pooled sensitivity and specificity of CEUS were 76% (95% confidence interval [CI]: 71%-81%; heterogeneity [I] = 74.5%) and 67% (95% CI: 62%-71%; I = 36.5%), respectively. The diagnostic odds ratio was 7.37 (95% CI: 3.78%-14.35; I = 66.6%). The overall area under the curve was 0.77 (standard error: 0.0392). Subgroup analysis revealed that different index tests of CEUS resulted in different diagnostic performance. Importantly, CEUS is an effective method for the differential diagnosis between benign and malignant STMs.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2020.08.011DOI Listing
December 2020

The role of miR-7 as a potential switch in the mouse hypothalamus-pituitary-ovary axis through regulation of gonadotropins.

Mol Cell Endocrinol 2020 12 8;518:110969. Epub 2020 Aug 8.

Department of Clinical Laboratory, Taizhou Second People's Hospital, Taizhou, Jiangsu, People's Republic of China. Electronic address:

The hypothalamus-pituitary-ovary (HPO) axis plays fundamental roles in female neuroendocrinology and reproduction. Pituitary gonadotropins are located in the center of this axis. Previous investigation suggested that miR-7 is closely linked with gonadotropins. However, the interaction between miR-7 and the HPO axis remains unclear. This study aims to determine whether and how miR-7 functions in this axis. A mouse ovariectomy model and mouse primary pituitary cells were used in this study. The results showed that miR-7 is localized to gonadotrophs and somatotrophs. miR-7 can inhibit the expression, synthesis and secretion of gonadotropins, but not growth hormones. Gonadotropin-releasing hormone (GnRH) has inhibitory effects on miR-7, while estrogen enhances miR-7 expression. miR-7 is vital for the pathway by which GnRH and estrogen regulate gonadotropins by targeting v-raf-leukemia viral oncogene 1 (Raf1). Together, these results indicate that miR-7 acts as a potential switch in the feedback loop of the HPO axis by regulating gonadotropins.
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http://dx.doi.org/10.1016/j.mce.2020.110969DOI Listing
December 2020

Light regulation of resistance to oxidative damage and magnetic crystal biogenesis in Magnetospirillum magneticum mediated by a Cys-less LOV-like protein.

Appl Microbiol Biotechnol 2020 Sep 11;104(18):7927-7941. Epub 2020 Aug 11.

Beijing Key Laboratory of Biological Electromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

Light-oxygen-voltage (LOV) proteins are ubiquitous photoreceptors that can interact with other regulatory proteins and then mediate their activities, which results in cellular adaptation and subsequent physiological changes. Upon blue-light irradiation, a conserved cysteine (Cys) residue in LOV covalently binds to flavin to form a flavin-Cys adduct, which triggers a subsequent cascade of signal transduction and reactions. We found a group of natural Cys-less LOV-like proteins in magnetotactic bacteria (MTB) and investigated its physiological functions by conducting research on one of these unusual LOV-like proteins, Amb2291, in Magnetospirillum magneticum. In-frame deletion of amb2291 or site-directive substitution of alanine-399 for Cys mutants impaired the protective responses against hydrogen peroxide, thereby causing stress and growth impairment. Consequently, gene expression and magnetosome formation were affected, which led to high sensitivity to oxidative damage and defective phototactic behaviour. The purified wild-type and A399C-mutated LOV-like proteins had similar LOV blue-light response spectra, but Amb2291 exhibited a faster reaction to blue light. We especially showed that LOV-like protein Amb2291 plays a role in magnetosome synthesis and resistance to oxidative stress of AMB-1 when this bacterium was exposed to red light and hydrogen peroxide. This finding expands our knowledge of the physiological function of this widely distributed group of photoreceptors and deepens our understanding of the photoresponse of MTB. KEY POINTS: • We found a group of Cys-less light-oxygen-voltage (LOV) photoreceptors in magnetotactic bacteria, which prompted us to study the light-response and biological roles of these proteins in these non-photosynthetic bacteria. • The Cys-less LOV-like protein participates in the light-regulated signalling pathway and improves resistance to oxidative damage and magnetic crystal biogenesis in Magnetospirillum magneticum. • This result will contribute to our understanding of the structural and functional diversity of the LOV-like photoreceptor and help us understand the complexity of light-regulated model organisms.
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http://dx.doi.org/10.1007/s00253-020-10807-5DOI Listing
September 2020