Publications by authors named "Ping Zhao"

1,139 Publications

  • Page 1 of 1

Mitochondria-targeted cyclometalated rhodium(III) complexes: synthesis, characterization and anticancer research.

Dalton Trans 2021 Jun 11. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Education Mega Centre, No. 280, Waihuandong Road, Guangzhou 510006, P.R. China.

Over the past few decades, the landscape of inorganic medicinal chemistry has been dominated by investigations on platinum or ruthenium, while the research based on other metal centers such as rhodium has been relatively insufficient. In this work, a series of cyclometalated rhodium(iii) complexes with imidazo[4,5-f][1,10]phenanthroline containing different aromatic rings were synthesized and characterized. Notably, all the complexes displayed stronger anticancer activity against various cancer cells compared with cisplatin. A mechanism study revealed that the rhodium complexes accumulated in the mitochondria, elevated the levels of mitochondrial reactive oxygen species (ROS) and released cytochrome c, indicating severe mitochondrial damage during the anticancer activity. Further studies illustrated that the rhodium complexes caused cell cycle arrest at the G2/M phase, upregulated the expression of p53 and reduced the ratio of B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated x (Bax), which ultimately resulted in cellular apoptosis. Overall, through mitochondrial pathways, these Rh(iii) complexes could induce cellular apoptosis to a larger extent than cisplatin and should be paid close attention as promising chemotherapeutic drugs in anticancer research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1dt01053kDOI Listing
June 2021

C, C pregnane glycosides and cytotoxic C spriostanol steroids from Asparagus cochinchinesis.

Steroids 2021 Jun 5;172:108874. Epub 2021 Jun 5.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, People's Republic of China. Electronic address:

A preliminary chemical investigation on 70% MeOH extract of the roots of Asparagus cochinchinensis resulted in the isolation of nine steroids. These isolates comprised of four new C (1-4) and one new pregnane (5) glycosides, and four known C (6-9) spirostanol steroids. Their structures were identified via analysis of the spectroscopic data and the results of hydrolytic cleavage. The cytotoxic activities of the compounds were tested toward the human tumor cell line Hela (cervical cancer), and compounds 7 and 8 displayed moderate activity with IC values of 35.5 and 39.6 μM, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.steroids.2021.108874DOI Listing
June 2021

Disrupted communication of the temporoparietal junction in patients with major depressive disorder.

Cogn Affect Behav Neurosci 2021 Jun 7. Epub 2021 Jun 7.

Department of Psychology, Renmin University of China, Beijing, 100872, China.

Patients with major depressive disorder (MDD) suffer impairment in the transmission and integration of internal and external information sources. Accumulating evidence suggests that the temporoparietal junction (TPJ) is important for multiple cognitive and social functions and may act as a key node for the integration of internal and external information. Therefore, the TPJ's aberrant interaction mechanism may underpin MDD psychopathology. To answer this question, we conducted a comprehensive study using resting-state functional magnetic imaging data recorded from 74 patients with MDD and 69 normal controls. First, we examined whether TPJ was the most prominent region with altered functional/effective connectivity with multiple depression-related regions/networks, based on either zero-lag correlations or temporal mutual information (total interdependence and Granger causality) measurements. Accordingly, we derived a network model that depicts alterations of TPJ-connectivity in patients with MDD. Lastly, we performed a cross-approach comparison demonstrating more conducive indicators in delineating the network alteration model. Functional/effective connectivity between the TPJ and major functional networks that govern internal and external-driven information resources was attenuated in patients with MDD. TPJ acts like a key node for information-inflow and integration of multiple information streams. Therefore, dysfunctional connectivity indicators may serve as effective biomarkers for MDD. MDD is associated with the breakdown of the TPJ interaction model and its connections with the default mode network and the task-positive network.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3758/s13415-021-00918-5DOI Listing
June 2021

Targeting PKM2 promotes chemosensitivity of breast cancer cells in vitro and in vivo.

Cancer Biomark 2021 Jun 5. Epub 2021 Jun 5.

Department of Breast Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Background: Pyruvate kinase M2 (PKM2) was overexpressed in many cancers, and high PKM2 expression was related with poor prognosis and chemoresistance.

Objective: We investigated the expression of PKM2 in breast cancer and analyzed the relation of PKM2 expression with chemotherapy resistance to the neoadjuvant chemotherapy (NAC). We also investigated whether PKM2 could reverse chemoresistance in breast cancer cells in vitro and in vivo.

Methods: Immunohistochemistry (IHC) was performed in 130 surgical resected breast cancer tissues. 78 core needle biopsies were collected from breast cancer patients before neoadjuvant chemotherapy. The relation of PKM2 expression and multi-drug resistance to NAC was compared. The effect of PKM2 silencing or overexpression on Doxorubicin (DOX) sensitivity in the MCF-7 cells in vitro and in vivo was compared.

Results: PKM2 was intensively expressed in breast cancer tissues compared to adjacent normal tissues. In addition, high expression of PKM2 was associated with poor prognosis in breast cancer patients. The NAC patients with high PKM2 expression had short survival. PKM2 was an independent prognostic predictor for surgical resected breast cancer and NAC patients. High PKM2 expression was correlated with neoadjuvant treatment resistance. High PKM2 expression significantly distinguished chemoresistant patients from chemosensitive patients. In vitro and in vivo knockdown of PKM2 expression decreases the resistance to DOX in breast cancer cells in vitro and tumors in vivo.

Conclusion: PKM2 expression was associated with chemoresistance of breast cancers, and could be used to predict the chemosensitivity. Furthermore, targeting PKM2 could reverse chemoresistance, which provides an effective treatment methods for patients with breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3233/CBM-210111DOI Listing
June 2021

Involvement of microRNA-155 in the mechanism of electroacupuncture treatment effects on experimental autoimmune encephalomyelitis.

Int Immunopharmacol 2021 Jun 3;97:107811. Epub 2021 Jun 3.

Department of Neurobiology, Harbin Medical University, No. 194 Xuefu Road, Harbin, Heilongjiang 150081, China; The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang 150081, China. Electronic address:

Multiple sclerosis (MS) is a neurodegenerative and demyelinating autoimmune disease mediated by autoreactive T cells that affects the central nervous system (CNS). Electroacupuncture (EA) has emerged as an alternative or supplemental treatment for MS, but the mechanism by which EA may alleviate MS symptoms is unresolved. Here, we examined the effects of EA at the Zusanli (ST36) acupoint on mice with experimental autoimmune encephalomyelitis (EAE), the predominant animal model of MS. The effects of EA on EAE emergence, inflammatory cell levels, proinflammatory cytokines, and spinal cord pathology were examined. EA treatment attenuated the EAE clinical score and associated spinal cord demyelination, while reducing the presence of proinflammatory cytokines in mononuclear cells (MNCs), downregulating microRNA (miR)-155, and upregulating the opioid peptide precursor proopiomelanocortin (POMC) in the CNS. Experiments in which cultured neurons were transfected with a miR-155 mimic or a miR-155 inhibitor further showed that the direct modulation of miR-155 levels could regulate POMC levels in neurons. In conclusion, the alleviation of EAE by EA is characterized by reduced proportions of Th1/Th17 cells and increased proportions of Th2 cells, POMC upregulation, and miR-155 downregulation, while miR-155 itself can suppress POMC expression. These results, support the hypothesis that the effects of EA on EAE may involve the downregulation of miR-155.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2021.107811DOI Listing
June 2021

Transcriptome analysis of under benomyl stress to discriminate the gene expression in response to development and immune systems.

J Environ Sci Health B 2021 Jun 3:1-12. Epub 2021 Jun 3.

Sericultural Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China.

The health and safety of the honeybees are seriously threatened due to the abuse of chemical pesticides in modern agriculture and apiculture. In this study, the RNA Seq approach was used to assess the effects of the honeybees treated with benomyl. The results showed that there were a total of 11,902 differentially expressed genes (DEGs). Among them, 5,759 DEGs were up-regulated and involved in the functions of immunity, detoxification, biological metabolism, and regulation. The DEGs were clustered in the GO terms of epidermal structure and response to external stimuli, and most of the DEGs were enriched in 15 pathways, such as light conduction, MAPK, calcium ion pathway, and so on. Moreover, the pathway of the toll signal transduction was activated. The data investigated that the expression of functional genes involved in the growth, development, foraging, and immunity of honeybees were significantly affected by benomyl stress, which would seriously threaten the health of the honeybees. This study provided a theoretical basis for revealing the response mechanism of honeybees to pesticides stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/03601234.2021.1930795DOI Listing
June 2021

Time-/Event-Triggered Adaptive Neural Asymptotic Tracking Control for Nonlinear Systems With Full-State Constraints and Application to a Single-Link Robot.

IEEE Trans Neural Netw Learn Syst 2021 Jun 2;PP. Epub 2021 Jun 2.

This study proposes the time-/event-triggered adaptive neural control strategies for the asymptotic tracking problem of a class of uncertain nonlinear systems with full-state constraints. First, we design a time-triggered strategy. The effect caused by the residuals of the estimation via radial basis function (RBF) neural networks (NNs), and the reasonable upper bounds on the first derivative of the reference signal and the derivative of each virtual control, can be eliminated by designing appropriate adaptive laws and utilizing the basic properties of RBF NNs. Moreover, the construction of the barrier Lyapunov functions (BLFs) in this work ensures the compliance of the full-state constraints and also holds the asymptotic output tracking performance. Then, based on the time-triggered strategy, we further design a relative threshold event-triggered strategy. The proposed event-triggered adaptive neural controller can solve the main control objective of this work, that is: 1) the full-state constraint requirements of the system are not violated and 2) the output signal asymptotically tracks the reference signal. Compared with the traditional method, the event-triggered strategy can improve the utilization of communication channels and resources and has greater practical significance. Finally, an example of single-link robot under the proposed two strategies illustrates the validity of the constructed controllers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3082994DOI Listing
June 2021

Red blood cell distribution width and serum CA-125 level as prognostic markers in acute exacerbation of chronic obstructive pulmonary disease.

J Int Med Res 2021 May;49(5):3000605211020229

Respiratory Department, Beijing Chaoyang District Shuangqiao Hospital, Beijing, China.

Objective: Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) is a primary cause of hospitalization and death in COPD. Serum CA-125 and red blood cell distribution width (RDW) are related to AECOPD. We investigated correlations between serum markers and AECOPD.

Methods: In total, 132 patients with AECOPD were included from January 2017 to December 2019. Participants were followed for 1 year. Patients were assigned to the poor prognosis (n = 40) or good prognosis (n = 92) group. We collected serum samples and general clinical information and conducted routine blood tests. We used logistic regression, receiver operating characteristic (ROC), and area under the ROC curve (AUC) analyses to assess differences between groups.

Results: We found significant differences between groups (odds ratio, 95% confidence interval) for age (1.046, 1.005-1.09), RDW (2.012, 1.339-3.023), and cancer antigen 125 (CA-125; 1.022, 1.006-1.039); these remained risk factors for AECOPD prognosis in multivariate analyses. RDW and CA-125 in combination was significant in ROC curve analysis. The AUC of RDW, CA-125, and these combined were 0.691, 0.779, and 0.772, respectively. Patients with RDW >12.75% and CA-125 >15.65 U/mL were predicted to have poor prognosis.

Conclusions: We found that RDW and CA-125 are potential prognostic indicators for AECOPD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/03000605211020229DOI Listing
May 2021

Dr. Hartmut Derendorf, PhD, a world-renowned expert in pharmacokinetics and pharmacometrics (1953-2020).

CPT Pharmacometrics Syst Pharmacol 2021 May 26. Epub 2021 May 26.

Integrated Development, Bill & Melinda Gates Foundation, Seattle, Washington, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/psp4.12609DOI Listing
May 2021

Immunomodulatory effects of anaesthetic sevoflurane in septic mouse model.

Saudi J Biol Sci 2021 May 18;28(5):2733-2738. Epub 2021 Mar 18.

Department of Anesthesiology, Weifang People's Hospital, Weifang, Shandong 261041, China.

Sepsis is one among the dangerous medical threat that is very much related to body's immune system having no proper treatment for this condition. About19 million cases of sepsis have been recorded and out of which 5 million cases die every year. Sevoflurane other than controlling the depth of anaesthesia, it does have a vital role in immunomodulations. The study is focused on investigating the immunomodulatory effects of sevoflurane in the septic mouse model induced by CLP. Mortality rate, organ damage, inflammatory mediators, bacterial load, coagulopathy, hepto and renal functional changes, serum lactate, blood glucose, neutrophil sequestration and finally histopathological examination were investigated. The results were interesting that exposure to sevoflurane improves the polymicrobial abdominal sepsis outcome. Mice exposed to sevoflurane after CLP significantly improved outcomes of polymicrobial abdominal sepsis and reduced mortality by improving overall 7-day survival (83.3%) compared to mice without sevoflurane (no treatment group 16.6%) additionally decreasing the surrogate marker levels in the experimental sepsis animal model conducted. Our study suggests that the selection of certain anaesthetic drugs could be critical in the management of septic patients because their immunomodulatory effects could be large enough to affect sepsis pathophysiology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sjbs.2021.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116997PMC
May 2021

Comment on: Randomized clinical trial on closure versus non-closure of mesenteric defects during laparoscopic gastric bypass surgery.

Br J Surg 2021 May 3. Epub 2021 May 3.

Department of Gastrointestinal Surgery, Sichuan Cancer Hospital & Research Institute, School of Medicine, University of Electronic Science and Technology of China (UESTC), Chengdu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/bjs/znab144DOI Listing
May 2021

Correction: Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation.

Biomater Sci 2021 Jun;9(11):4212-4213

Biological Science Research Center, Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715, People's Republic of China.

Correction for 'Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation' by Feng Wang et al., Biomater. Sci., 2020, 8, 657-672, DOI: 10.1039/C9BM01478K.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1bm90052hDOI Listing
June 2021

EphA2: A promising therapeutic target in breast cancer.

J Genet Genomics 2021 Mar 29. Epub 2021 Mar 29.

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China; KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China; Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, 510095, China. Electronic address:

EphA2 (ephrin type-A receptor 2), a receptor tyrosine kinase, is overexpressed in human breast cancers often linked to poor patient prognosis. Accumulating evidence demonstrates that EphA2 plays important roles in several critical processes associated with malignant breast progression, such as proliferation, survival, migration, invasion, drug resistance, metastasis, and angiogenesis. As its inhibition through multiple approaches can inhibit the growth of breast cancer and restore drug sensitivity, EphA2 has become a promising therapeutic target for breast cancer treatment. Here, we summarize the expression, functions, mechanisms of action, and regulation of EphA2 in breast cancer. We also list the potential therapeutic strategies targeting EphA2. Furthermore, we discuss the future directions of studying EphA2 in breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2021.02.011DOI Listing
March 2021

Estrogen-Related Receptor Influences the Hemolymph Glucose Content by Regulating Midgut Trehalase Gene Expression in the Last Instar Larvae of .

Int J Mol Sci 2021 Apr 21;22(9). Epub 2021 Apr 21.

Biological Science Research Center, Southwest University, Chongqing 400716, China.

The expression of trehalase in the midgut of insects plays an important role in glucose supply to the hemolymph. Energy metabolism is usually regulated by the estrogen-related receptor (ERR). A decrease in ATP levels is caused by the ERR hindering glycolysis. However, the relationship between trehalose accumulation and ERR expression is still unclear. Here, we found that silkworm ERR (BmERR) is concentrated and BmERR expression is strongly correlated with trehalase in the midgut during the last instar silkworm larval stage. We cloned the promoter of the trehalase from (BmTreh) and found that the ERR bound directly to the core response elements of the promoter. Cell level interference and the overexpression of ERR can reduce or enhance BmTreh transcription and promoter activity. Overexpressed transgenic BmERR can significantly increase the expression of BmTreh in the midgut of the last instar silkworm larvae, thereby hydrolyzing trehalose into glucose and releasing it into the hemolymph. Additionally, increased hemolymph glucose content reduces silkworm pupa weight but does not affect silk protein production from the silk gland. Our results suggest a novel function for BmERR through its involvement in BmTreh regulation and expand the understanding of ERR functions in insect trehalose metabolism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22094343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122577PMC
April 2021

Association between serum uric acid and bone mineral density in patients with type 2 diabetes: A 6-year longitudinal study in China.

Medicine (Baltimore) 2021 Apr;100(17):e25733

Beijing Xiao-Tang-Shan Hospital, Xiao-Tang-Shan Town, Changping District, Beijing, China.

Abstract: The relationship between serum uric acid (UA) and bone mineral density (BMD) has been proposed by several researchers. However, there has been no consensus regarding the relationships among serum UA, diabetes, and BMD. The aim of this study is to investigate the association between UA, BMD, and at least osteopenia in type 2 diabetes patients.This research was a longitudinal study performed at Xiao-Tang-Shan Hospital in Beijing. Type 2 diabetes diagnosis was consistent with the WHO standard classification. Participants with osteopenia or osteoporosis documented by dual-energy X-ray absorptiometry were defined as having "at least osteopenia." A generalized additive model and multivariable logistic regressions were performed to explore the relationship between serum UA and at least osteopenia. Receiver operating characteristic analysis was conducted. Propensity score matching was used to verify the correctness of the cutoff point.In total, 3476 type 2 diabetes patients free of any osteopenia-related diseases were recruited in 2012 and followed up to 2018. The general proportions of patients with at least osteopenia in 2018 was 16.46% (572/3476). Serum UA was negatively associated with BMD stratified by sex, age group, and BMI level. Setting the first quartile as the reference, the risk of at least osteopenia in the fourth quartile was significant among all patients (odds ratio [OR]: 0.75; 95% confidence interval [CI]: 0.57, 0.98) and specifically in females (OR: 0.79; 95% CI: 0.43, 0.97), patients aged over 50 years (OR: 0.79; 95% CI: 0.60, 0.97) and patients with a BMI greater than 25 (OR: 0.74; 95% CI: 0.47, 0.97). The optimal cutoff point for the serum UA level to distinguish at least osteopenia in diabetic patients was 395 μmol/L.Serum UA concentration is negatively associated with the occurrence of at least osteopenia in Chinese patients with type 2 diabetes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084059PMC
April 2021

Association between blood urea nitrogen and incidence of type 2 diabetes mellitus in a Chinese population: a cohort study.

Endocr J 2021 Apr 28. Epub 2021 Apr 28.

Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China.

To examine the association between blood urea nitrogen (BUN) and risk of type 2 diabetes (T2DM) among Chinese adults, we performed an ongoing cohort study of 38578 Chinese adults (56.3% males; average age, 41.6 y) who underwent repeated health check-up examinations between 2009 and 2016 and without T2DM at baseline. During follow-up, incident T2DM cases were identified based on self-report, medication use, measurements of fasting plasma glucose, 2 h post oral glucose, or haemoglobinA1c. 2009 (5.2%) cases confirmed with incident T2DM were identified during median follow-up of 3.1 years. With increasing quartiles of BUN levels, the incidences of T2DM gradually increased with 0.69%, 1.11%, 1.53%, and 1.87% for quartile 1 to quartile 4 (p trend <0.001). Compared with quartile 1, the multivariate-adjusted hazard ratios (HRs) and its 95% confidence intervals (95% CIs) for T2DM risk were 1.16 (0.97-1.38) for quartile 2, 1.28 (1.07-1.51) for quartile 3, and 1.28 (1.08-1.52) for quartile 4 (p trend = 0.005). HR for per each standard deviation increase in BUN level was 1.10 (1.04-1.16) (p trend <0.001). This association tended to be more pronounced in those with a lower body mass index at baseline (p-interaction <0.001). Our results suggested that BUN levels were positively associated with incident T2DM risk among Chinese adults. Future prospective investigations in other populations are necessary to confirm our findings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1507/endocrj.EJ20-0794DOI Listing
April 2021

Microfluidic Model to Mimic Initial Event of Neovascularization.

J Vis Exp 2021 04 10(170). Epub 2021 Apr 10.

Beijing Advanced Innovation Centre for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Chinese Education Ministry, School of Biological Science and Medical Engineering, Beihang University; School of Engineering Medicine, Beihang University;

Neovascularization is usually initialized from an existing normal vasculature and the biomechanical microenvironment of endothelial cells (ECs) in the initial stage varies dramatically from the following process of neovascularization. Although there are plenty of models to simulate different stages of neovascularization, an in vitro 3D model that capitulates the initial process of neovascularization under the corresponding stimulations of normal vasculature microenvironments is still lacking. Here, we reconstructed an in vitro 3D model that mimics the initial event of neovascularization (MIEN). The MIEN model contains a microfluidic sprouting chip and an automatic control, highly efficient circulation system. A functional, perfusable microchannel coated with endothelium was formed and the process of sprouting was simulated in the microfluidic sprouting chip. The initially physiological microenvironment of neovascularization was recapitulated with the microfluidic control system, by which ECs would be exposed to high luminal shear stress, physiological transendothelial flow, and various vascular endothelial growth factor (VEGF) distributions simultaneously. The MIEN model can be readily applied to the study of neovascularization mechanism and holds a potential promise as a low-cost platform for drug screening and toxicology applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3791/62003DOI Listing
April 2021

Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.

Commun Biol 2021 Apr 22;4(1):491. Epub 2021 Apr 22.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. We identified a distinctive class of histidine rich (His-rich) CPs (6%-45%) from developing lepidopteran scales by LC-MS/MS. Functional studies using RNAi revealed CPs with different histidine content play distinct and critical roles in constructing the microstructure of the scale surface. Moreover, we successfully synthesized films in vitro by crosslinking a 45% His-rich CP (BmorCPR152) with laccase2 using N-acetyl- dopamine or N-β-alanyl-dopamine as the substrate. This molecular study of scales provides fundamental information about how such a fine microstructure is constructed and insights into the potential application of CPs as new biomaterials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s42003-021-01996-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062583PMC
April 2021

Effect of a low-cost and highly efficient passivator synthesized by alkali-fused fly ash and swine manure on the leachability of heavy metals in a multi-metal contaminated soil.

Chemosphere 2021 Sep 12;279:130558. Epub 2021 Apr 12.

Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong, 525000, China. Electronic address:

Soil pollution, caused by heavy metals, is an environmental problem that requires an urgent solution in China. Chemical passivation is a technology that uses various passivators to reduce the availability of heavy metals in soil and realize the remediation of contaminated soil. In this study, we examined the effects of fly ash (FA), alkali-fused fly ash (AFFA), swine manure biochar (SB), and modifying biochar (MB) on the leachability of Cu, Zn, Pb, and Cd via soil culture experiments. The results showed that the addition of AFFA, SB, and MB significantly reduced the extractable contents of Cu, Pb, and Cd in the soil. AFFA and MB had the best passivation effect, followed by SB and FA. The passivation effect on Pb was the best, followed by that on Cu and Cd. AFFA modification significantly improved the passivation effect of MB on Cu, Pb, and Cd in composite contaminated soil. With the addition of 3% MB, the Pb, Cu, and Cd extracted by TCLP decreased by 95.7, 74.1, and 59.1%, respectively. Correlation analysis, Fourier transform infrared spectrometry, scanning electron microscopy, and energy-dispersive X-ray spectroscopy showed that the passivation mechanism is mainly due to an increase in the soil pH, silicate content, and cohesiveness. The soil culture experiments in this study proved that MB is a low-cost and highly efficient organic-inorganic composite passivator for multi-metal contaminated soils.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130558DOI Listing
September 2021

SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination.

Cell Death Differ 2021 Apr 20. Epub 2021 Apr 20.

Laboratory of Cell Engineering, Institute of Biotechnology, Beijing, China.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is highly contagious and causes lymphocytopenia, but the underlying mechanisms are poorly understood. We demonstrate here that heterotypic cell-in-cell structures with lymphocytes inside multinucleate syncytia are prevalent in the lung tissues of coronavirus disease 2019 (COVID-19) patients. These unique cellular structures are a direct result of SARS-CoV-2 infection, as the expression of the SARS-CoV-2 spike glycoprotein is sufficient to induce a rapid (~45.1 nm/s) membrane fusion to produce syncytium, which could readily internalize multiple lines of lymphocytes to form typical cell-in-cell structures, remarkably leading to the death of internalized cells. This membrane fusion is dictated by a bi-arginine motif within the polybasic S1/S2 cleavage site, which is frequently present in the surface glycoprotein of most highly contagious viruses. Moreover, candidate anti-viral drugs could efficiently inhibit spike glycoprotein processing, membrane fusion, and cell-in-cell formation. Together, we delineate a molecular and cellular rationale for SARS-CoV-2 pathogenesis and identify novel targets for COVID-19 therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41418-021-00782-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056997PMC
April 2021

LncRNA MYCNOS promotes glioblastoma cell proliferation by regulating miR-216b/FOXM1 axis.

Metab Brain Dis 2021 Apr 19. Epub 2021 Apr 19.

Department of Pediatric Surgery, Gansu Provincial Maternity and Child-Care Hospital, No.143 Qilihe North Street, Lanzhou City, Gansu Province, 730050, People's Republic of China.

MYCNOS is an oncogenic lncRNA in liver cancer, but its role in glioblastoma (GBM) is unknown. We predicted that MYCNOS might interact with miR-216b, which targets FOXM1 to perform tumor suppressive roles. This study was performed to analyze the role of MYCNOS in GBM and explore its potential interactions with miR-216b and FOXM1. MYCNOS expression in paired GBM and non-tumor tissues from 62 GBM patients was analyzed by RT-qPCR. The interaction between MYCNOS and miR-216b was predicted by IntaRNA 2.0 and confirmed by dual luciferase activity assay. Overexpression of MYCNOS, miR-216b, and FOXM1 was achieved in GBM cells, followed by performing RT-qPCR and Western blot to explore the relationship among them. CCK-8 assay was performed to explore the role of MYCNOS, miR-216b, and FOXM1 in regulating GBM cell proliferation. MYCNOS was upregulated in GBM tissues compared to the paired non-tumor tissues. MYCNOS is predicted to interact with miR-216b, but overexpression of MYCNOS and miR-216b failed to affect each other's expression significantly. Dual luciferase activity assay showed that MYCNOS and miR-216b could directly interact with each other. MYCNOS overexpression increased the expression of FOXM1, which is a direct target of miR-216b. Cell proliferation assay showed that MYCNOS and FOXM1 overexpression resulted in an increased proliferation rate of GBM cells, while miR-216b overexpression suppressed cell proliferation. Moreover, MYCNOS overexpression suppressed the role of miR-216b. MYCNOS may regulate FOXM1 expression of by serving as an endogenous sponge of miR-216b axis to promote the proliferation of GBM cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11011-021-00729-0DOI Listing
April 2021

Co-Immunization With CHIKV VLP and DNA Vaccines Induces a Promising Humoral Response in Mice.

Front Immunol 2021 24;12:655743. Epub 2021 Mar 24.

National Health Commission (NHC) Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.

Chikungunya fever is an acute infectious disease that is mediated by the mosquito-transmitted chikungunya virus (CHIKV), for which no licensed vaccines are currently available. Here, we explored several immunization protocols and investigated their immunity and protective effects in mice, with DNA- and virus-like particle (VLP)- vaccines, both alone and in combination. Both DNA and VLP vaccine candidates were developed and characterized, which express CHIKV structural genes (C-E3-E2-6K-E1). Mice were immunized twice, with different protocols, followed by immunological detection and CHIKV Ross challenge. The highest antigen-specific IgG and neutralizing activity were induced by DNA and VLP co-immunization, while the highest cellular immunity was induced by DNA vaccination alone. Although all vaccine groups could protect mice from lethal CHIKV challenge, demonstrated as reduced viral load in various tissues, without weight loss, mice co-immunized with DNA and VLP exhibited the mildest histopathological changes and lowest International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) scores, in comparison to mice with either DNA or VLP vaccination alone. We concluded that co-immunization with DNA and VLP is a promising strategy to inducing better protective immunity against CHIKV infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.655743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044884PMC
March 2021

Natural history of lung function over one year in patients with Parkinson's disease.

Respir Med 2021 Jun 16;182:106396. Epub 2021 Apr 16.

Acorda Therapeutics, Inc., Ardsley, NY, USA.

Background: Little is known about decline in lung function in Parkinson's disease (PD). To assess these changes, we assessed the changes in lung function that occurred over 12 months in patients on standard PD therapy as part of the observational cohort of an open-label study of inhaled levodopa (CVT-301) in PD.

Methods: PD patients on stable oral PD therapy and no chronic respiratory disease had spirometry and diffusing capacity of the lungs for carbon monoxide (DL) measured at 3, 6, 9, and 12 months.

Results: 106 patients (81.5%) in the observational cohort on no investigational therapy completed the study. Mean FEV declined at 12 months from 2.88L at baseline with a mean change of -0.11L, greater than the -0.030-0.045L/year observed in healthy, non-smokers aged 60-70 years. FVC declined from 3.77L (mean change -0.19L); FEV/FVC ratio remained relatively constant. DL mean change was -0.48 mL/min/mmHg from a baseline of 24.24 mL/min/mmHg. This change in DL, while not significant, was similar to that seen in non-smokers aged 60-70 years (DL -0.42-0.63 mL/min/mmHg/year). Decreases in alveolar volume (VA) and inspiratory vital capacity (IVC) rather than the transfer coefficient (DL/VA) were observed.

Conclusions: PD patients had greater declines in FEV, and FVC, but not in DL compared to healthy non-smokers of similar age. Declines in FEV and FVC with little change in FEV/FVC, and decline in VA and IVC with little change in DL/VA, suggest these changes were due to decreases in lung volume and are compatible with progressive PD-associated respiratory muscle weakness.

Trial Registration: ClinicalTrials.gov (NCT02352363 Registered January 26, 2015 [https://clinicaltrials.gov/ct2/show/NCT02352363]) and EudraCT (2014-003799-22).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rmed.2021.106396DOI Listing
June 2021

Exploring communication between the thalamus and cognitive control-related functional networks in the cerebral cortex.

Cogn Affect Behav Neurosci 2021 Apr 17. Epub 2021 Apr 17.

Department of Psychology, Renmin University of China, Beijing, 100872, China.

It has been suggested by multiple studies (postmortem studies, invasive animal studies, and diffusion tensor imaging in the human brain) that the thalamus is important for communication among cortical regions. Many functional magnetic resonance imaging (fMRI) studies, including noninvasive and whole-brain studies, have reported thalamic co-activation with several cognitive control-related cortical systems. This forms a complex network that may be important for advanced cognitive control-related processes, such as working memory and attention. Nevertheless, how the thalamus communicates with the cognitive control-related network in the intact human brain is an essential question and needs further investigation. To address this question, we conducted a study using dynamic functional connectivity analysis and effective connectivity analysis based on fMRI data from young, healthy adult participants. The results showed that the middle thalamus exhibited both high in- and out-degree regarding the complex network related to cognitive control during both rest and task conditions. Furthermore, intrinsic communication via the middle thalamic regions showed dynamically co-varying patterns, and the thalamic regions showed high flexibility in dynamic community analysis. These results indicated that the mid-thalamic region is an important station for communication between nodes in cognitive control-related networks.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3758/s13415-021-00892-yDOI Listing
April 2021

Homeodomain proteins POU-M2, antennapedia and abdominal-B are involved in regulation of the segment-specific expression of the clip-domain serine protease gene CLIP13 in the silkworm, Bombyx mori.

Insect Sci 2021 Apr 16. Epub 2021 Apr 16.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.

Clip-domain serine proteases (CLIPs) play important roles in insect innate immunity and development. Our previous studies indicated that CLIP13, an epidermis-specific gene, was involved in cuticle remodeling during molting and metamorphosis in the silkworm, Bombyx mori. However, the transcriptional regulatory mechanism and regulatory pathways of CLIP13 remained unclear. In the present study, we investigated CLIP13 expression and the regulation pathway controlled by 20-hydroxyecdysone (20E) in the silkworm. At the transcriptional level, expression of CLIP13 exhibited pronounced spatial and temporal specificity in different regions of the epidermis; homeodomain transcription factors POU-M2, antennapedia (Antp), and abdominal-B (Abd-B) showed similar expression change trends as CLIP13 in the head capsule, thorax, and abdomen, respectively. Furthermore, results of cell transfection assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation demonstrated that POU-M2, Antp, and Abd-B were involved in the transcriptional regulation of CLIP13 by directly binding to their cis-response elements in CLIP13 promoter. RNA interference-mediated silencing of POU-M2, Antp, and Abd-B led to a decrease of CLIP13 expression in the head capsule, the epidermis of the 1st to 3rd thoracic segments and the 7th to 10th abdominal segments, respectively. Consistent with CLIP13, 20E treatment significantly upregulated expression of POU-M2, Antp, and Abd-B in the silkworm epidermis. Taken together, these data suggest that 20E positively regulates transcription of CLIP13 via homeodomain proteins POU-M2, Antp, and Abd-B in different regions of the silkworm epidermis during metamorphosis, thus affecting the molting process. Our findings provide new insight into the functions of homeodomain transcription factors in insect molting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1744-7917.12916DOI Listing
April 2021

Spectroscopic and in silico investigation of the interaction between GH1 β-glucosidase and ginsenoside Rb.

Food Sci Nutr 2021 Apr 9;9(4):1917-1928. Epub 2021 Feb 9.

College of Food Science and Engineering Jilin University Changchun China.

The function and application of β-glucosidase attract attention nowadays. β-glucosidase was confirmed of transforming ginsenoside Rb to rare ginsenoside, but the interaction mechanism remains not clear. In this work, β-glucosidase from GH1 family of Paenibacillus was selected, and its gene sequence was synthesized by codon. Then, recombinant plasmid was transferred into BL21 (DE3) and expressed. The UV-visible spectrum showed that ginsenoside Rb decreased the polarity of the corresponding structure of hydrophobic aromatic amino acids (Trp) in β-glucosidase and increased new π-π transition. The fluorescence quenching spectrum showed that ginsenoside Rb inhibited intrinsic fluorescence, formed static quenching, reduced the surface hydrophobicity of β-glucosidase, and K was 8.37 × 10 L/M (298K). Circular dichroism (CD) showed that secondary structure of β-glucosidase was changed by the binding action. Localized surface plasmon resonance (LSPR) showed that β-glucosidase and Rb had strong binding power which KD value was 5.24 × 10 (±2.35 × 10) M. Molecular docking simulation evaluated the binding site, hydrophobic force, hydrogen bond, and key amino acids of β-glucosidase with ginsenoside Rb in the process. Thus, this work could provide basic mechanisms of the binding and interaction between β-glucosidase and ginsenoside Rb.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.2153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020931PMC
April 2021

The functional hierarchy of the task-positive networks indicates a core control system of top-down regulation in visual attention.

J Integr Neurosci 2021 Mar;20(1):43-53

Department of Psychology, Renmin University of China, 100872 Beijing, P. R. China.

The cingulo-opercular network (CON), dorsal attention network (DAN), and ventral attention network (VAN) are prominently activated during attention tasks. The function of these task-positive networks and their interplay mechanisms in attention is one of the central issues in understanding how the human brain manipulates attention to better adapt to the external environment. This study aimed to clarify the CON, DAN, and VAN's functional hierarchy by assessing causal interactions. Functional magnetic resonance imaging (fMRI) data from human participants performing a visual-spatial attention task and correlating Granger causal influences with behavioral performance revealed that CON exerts behavior-enhancing influences upon DAN and VAN, indicating a higher level of CON in top-down attention control. By contrast, the VAN exerts a behavior-degrading influence on CON, indicating external disruption of the CON's control set.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.31083/j.jin.2021.01.297DOI Listing
March 2021

Integrative Taxonomy of the Spinous Assassin Bug Genus (Heteroptera: Reduviidae: Harpactorinae) Reveals Three Cryptic Species Based on DNA Barcoding and Morphological Evidence.

Insects 2021 Mar 16;12(3). Epub 2021 Mar 16.

MOA Key Lab of Pest Monitoring and Green Management, Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Stål, 1861 (Heteroptera: Reduviidae: Harpactorinae) is endemic to China and Vietnam, with only two species, Stål, 1861 and Ren, 2001, characterized by spinous body and dentate abdominal connexivum. However, due to variable morphological characteristics, , which is widely distributed in South China, is possibly a complex of cryptic species, and was suspected to be an extreme group of the cline. In the present study, we conducted species delimitation and phylogenetic analyses based on the mitochondrial cytochrome c oxidase subunit I () gene sequences of 307 specimens collected from 30 sampling localities combined with morphological evidence. The result showed that all samples used in this study were identified as five species: is a valid species, and actually includes three cryptic species: P. Zhao and Cai sp. nov., P. Zhao and Cai sp. nov., and P. Zhao and Cai sp. nov. In this paper, the genus is systematically revised, and the morphological characteristics of the five species are compared, described, and photographed in detail. We elucidate the evolutionary history of based on results of estimated divergence time. The body shape and coloration (green in nymph and brown in adult) of match their environment and mimic the plants on which they live. The symbiotic relationship between and spinous plants is presented and discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/insects12030251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001484PMC
March 2021

Improvement of Shen'ge formula on heart function in diastolic heart failure: A protocol for randomized, double-blind, placebo-controlled clinical study.

Medicine (Baltimore) 2021 Apr;100(13):e25383

Department of cardiology, Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine. Shanghai, PR China.

Introduction: Diastolic heart failure (DHF) is an important pathological type of heart failure, that involves multiple organ dysfunction and multiple complications. The prevalence of DHF is high, and effective treatments are lacking. Chinese herbs are an alternative therapy for DHF. Shen'ge formula (SGF) is a classical formula from which patients can benefit, but convincing evidence of its efficacy is lacking. Therefore, we designed this randomized controlled trial protocol.

Methods/design: This randomized, double-blind, placebo-controlled clinical trial will evaluate the efficacy and safety of SGF in the treatment of DHF. A total of 130 patients with DHF will be enrolled in the trial and treated with SGF granules or placebo for 12 weeks and followed up for 12 weeks. The primary outcome measurement will be to changes in plasma N-terminal brain natriuretic peptide precursor before versus after treatment, while the second primary outcome measurement will be changes in heart function before versus after treatment and the 12-week follow-up period. It will also include echocardiography, a cardiopulmonary exercise test, cardiac function grading, traditional Chinese medicine syndrome score, and the Minnesota Heart Failure Quality of Life Scale. Adverse events will be evaluated throughout the trial.

Discussion: The results of this trial will demonstrate whether SGF could alleviate symptoms, improve cardiac function, reduce readmission rates, and improve quality of life of patients with DHF.

Trial Registration: Chinese Clinical Trial Register, ChiCTR2000036533, registered on August 24, 2020.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021288PMC
April 2021

Molecular basis of cross-resistance to acetohydroxy acid synthase-inhibiting herbicides in Sagittaria trifolia L.

Pestic Biochem Physiol 2021 Mar 29;173:104795. Epub 2021 Jan 29.

College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning 110866, China. Electronic address:

Acetohydroxy acid synthase (AHAS)-inhibiting herbicides are one of the most commonly used herbicides for controlling the growth of Sagittaria trifolia L. in paddy fields in Northeastern China. In this study, we collected five suspected resistant populations of S. trifolia (R1-R5) from three different provinces of Northeastern China. The results of whole-plant bioassays revealed that those populations showed high level of resistance to bensulfuron-methyl with resistance index (GR R/S) ranging from 39.90 to 88.50. The results of AHAS-activity assays were consistent with the results of the whole-plant bioassays. The AHAS gene analysis showed that R2 and R3 populations contained Pro-197-Leu mutations that were highly resistant to penoxsulam; R1 and R4 populations contained Pro-197-Ser mutations that were highly resistant to bispyribac‑sodium; R5 population contained Trp-574-Leu mutation that showed high resistance to IMI, PT, PTB and SU herbicides. The AHAS with resistance mutations showed less sensitivity to feedback inhibition by BCAAs and R genotypes had increased free BCAAs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pestbp.2021.104795DOI Listing
March 2021