Publications by authors named "Ping Zhang"

3,468 Publications

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Transverse oscillating bubble enhanced laser-driven betatron X-ray radiation generation.

Sci Rep 2022 Jun 27;12(1):10855. Epub 2022 Jun 27.

Department of Physics and Astronomy, University of Nebraska - Lincoln, Lincoln, NE, 68588, USA.

Ultrafast high-brightness X-ray pulses have proven invaluable for a broad range of research. Such pulses are typically generated via synchrotron emission from relativistic electron bunches using large-scale facilities. Recently, significantly more compact X-ray sources based on laser-wakefield accelerated (LWFA) electron beams have been demonstrated. In particular, laser-driven sources, where the radiation is generated by transverse oscillations of electrons within the plasma accelerator structure (so-called betatron oscillations) can generate highly-brilliant ultrashort X-ray pulses using a comparably simple setup. Here, we experimentally demonstrate a method to markedly enhance the parameters of LWFA-driven betatron X-ray emission in a proof-of-principle experiment. We show a significant increase in the number of generated photons by specifically manipulating the amplitude of the betatron oscillations by using our novel Transverse Oscillating Bubble Enhanced Betatron Radiation scheme. We realize this through an orchestrated evolution of the temporal laser pulse shape and the accelerating plasma structure. This leads to controlled off-axis injection of electrons that perform large-amplitude collective transverse betatron oscillations, resulting in increased radiation emission. Our concept holds the promise for a method to optimize the X-ray parameters for specific applications, such as time-resolved investigations with spatial and temporal atomic resolution or advanced high-resolution imaging modalities, and the generation of X-ray beams with even higher peak and average brightness.
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http://dx.doi.org/10.1038/s41598-022-14748-zDOI Listing
June 2022

DeepDRiD: Diabetic Retinopathy-Grading and Image Quality Estimation Challenge.

Patterns (N Y) 2022 Jun 20;3(6):100512. Epub 2022 May 20.

Department of Computer Science and Engineering, The Ohio State University, Ohio, USA.

We described a challenge named "Diabetic Retinopathy (DR)-Grading and Image Quality Estimation Challenge" in conjunction with ISBI 2020 to hold three sub-challenges and develop deep learning models for DR image assessment and grading. The scientific community responded positively to the challenge, with 34 submissions from 574 registrations. In the challenge, we provided the DeepDRiD dataset containing 2,000 regular DR images (500 patients) and 256 ultra-widefield images (128 patients), both having DR quality and grading annotations. We discussed details of the top 3 algorithms in each sub-challenges. The weighted kappa for DR grading ranged from 0.93 to 0.82, and the accuracy for image quality evaluation ranged from 0.70 to 0.65. The results showed that image quality assessment can be used as a further target for exploration. We also have released the DeepDRiD dataset on GitHub to help develop automatic systems and improve human judgment in DR screening and diagnosis.
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http://dx.doi.org/10.1016/j.patter.2022.100512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214346PMC
June 2022

Epigenomic analysis reveals a dynamic and context-specific macrophage enhancer landscape associated with innate immune activation and tolerance.

Genome Biol 2022 Jun 24;23(1):136. Epub 2022 Jun 24.

Chinese Academy of Medical Science Oxford Institute, University of Oxford, Oxford, UK.

Background: Chromatin states and enhancers associate gene expression, cell identity and disease. Here, we systematically delineate the acute innate immune response to endotoxin in terms of human macrophage enhancer activity and contrast with endotoxin tolerance, profiling the coding and non-coding transcriptome, chromatin accessibility and epigenetic modifications.

Results: We describe the spectrum of enhancers under acute and tolerance conditions and the regulatory networks between these enhancers and biological processes including gene expression, splicing regulation, transcription factor binding and enhancer RNA signatures. We demonstrate that the vast majority of differentially regulated enhancers on acute stimulation are subject to tolerance and that expression quantitative trait loci, disease-risk variants and eRNAs are enriched in these regulatory regions and related to context-specific gene expression. We find enrichment for context-specific eQTL involving endotoxin response and specific infections and delineate specific differential regions informative for GWAS variants in inflammatory bowel disease and multiple sclerosis, together with a context-specific enhancer involving a bacterial infection eQTL for KLF4. We show enrichment in differential enhancers for tolerance involving transcription factors NFκB-p65, STATs and IRFs and prioritize putative causal genes directly linking genetic variants and disease risk enhancers. We further delineate similarities and differences in epigenetic landscape between stem cell-derived macrophages and primary cells and characterize the context-specific enhancer activities for key innate immune response genes KLF4, SLAMF1 and IL2RA.

Conclusions: Our study demonstrates the importance of context-specific macrophage enhancers in gene regulation and utility for interpreting disease associations, providing a roadmap to link genetic variants with molecular and cellular functions.
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http://dx.doi.org/10.1186/s13059-022-02702-1DOI Listing
June 2022

Enantioselective Conjugate Addition of Boronic Acids to α,β-Unsaturated 2-Acyl Imidazoles Catalyzed by Chiral Diols.

J Org Chem 2022 Jun 24. Epub 2022 Jun 24.

NMPA Key Laboratory for Research and Evaluation of Innovative Drug, Henan Key Laboratory of Organic Functional Molecules and Drug Innovation, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, China.

Herein, we report the enantioselective conjugate addition of organic boronic acids to α,β-unsaturated 2-acyl imidazoles using ()-3,3'-I-BINOL as the catalyst. The catalytic system shows high efficiency and tolerance to alkenylboronic acids and heteroarylboronic acids. The corresponding Michael addition products were obtained in moderate to excellent yields and with moderate to excellent enantioselectivities (up to 97% ee). A gram-scale reaction was also conducted, and the desired product was obtained in high yield with no erosion in enantioselectivity. Finally, the synthetic utility of the methodology was demonstrated by transforming the 2-acyl imidazole moiety to the corresponding aldehyde, carboxylic acid, ester, and amide derivatives.
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http://dx.doi.org/10.1021/acs.joc.2c00929DOI Listing
June 2022

Correction: The hangman effect boosts hydrogen production by a manganese terpyridine complex.

Chem Commun (Camb) 2022 Jun 24. Epub 2022 Jun 24.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, P. R. China.

Correction for 'The hangman effect boosts hydrogen production by a manganese terpyridine complex' by Qianqian Wu , , 2022, , 5128-5131, https://doi.org/10.1039/D2CC00757F.
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http://dx.doi.org/10.1039/d2cc90224aDOI Listing
June 2022

Bifunctional Phenol-enabled Sequential Polymerization Strategy for Printable Tough Hydrogels.

Macromol Rapid Commun 2022 Jun 24:e2200419. Epub 2022 Jun 24.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, 710069, China.

Hydrogels are promising material candidates in engineering soft robotics, mechanical sensors, biomimetic regenerative medicine, etc. However, developing multinetwork hydrogels with high mechanical properties and excellent printability is still challenging. Here, we report a bifunctional phenol-enabled sequential polymerization (BPSP) strategy to fabricate high-performance multinetwork hydrogels under the orthogonal catalysis of efficient ruthenium photochemistry. Benefiting from this bifunctional design, phenols can sequentially polymerize with typical monomers and themselves to fabricate various phenol-containing polymers (Ph-Ps) and Ph-Ps-based multinetwork tough hydrogels, respectively. The as-prepared hydrogels have maximum stress of 0.75 MPa and toughness of 2.2 MJ/m under the critical strain of 800%. These property parameters are a maximum of 16 times higher than that of the phenol-postmodified and phenol-free hydrogels. Moreover, the rapid coupling polymerization of phenols can shorten the gelation times of hydrogels to as low as ∼4 s, which enables its printable property for customizable applications. As a proof of concept, a 3D scaffold-like structure is optimized as highly sensitive mechanical sensors for detecting various human motions. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/marc.202200419DOI Listing
June 2022

Design Methodology of Automotive Time-Sensitive Network System Based on OMNeT++ Simulation System.

Sensors (Basel) 2022 Jun 17;22(12). Epub 2022 Jun 17.

Nanchang Automotive Institute of Intelligence and New Energy, Tongji University (NAIT), Nanchang 330052, China.

Advances in automotive technology require networks to support a variety of communication requirements, such as reliability, real-time performance, low jitter, and strict delay limits. Time-Sensitive Network (TSN) is a keyframe transmission delay-guaranteed solution based on the IEEE 802 architecture of the automotive Ethernet. However, most of the existing studies on automotive TSN performance are based on a single mechanism, lacking a complete and systematic research tool. At the same time, the design method should be considered from a global perspective when designing an automotive TSN system, rather than only considering a single mechanism that TSN applies to. This paper discusses the correspondence between traffic types and automotive scenarios and proposes a methodology to target the delay constraint of traffic types as the design goal of automotive TSN networks. To study the performance of automotive TSN under different mechanisms such as time-aware shaper (TAS), credit-based shaper (CBS), cyclic queuing and forwarding (CQF), etc., this paper also develops a systematic automotive TSN simulation system based on OMNeT++. The simulation system plays a crucial role in the whole methodology, including all applicable TSN standards for the automotive field. Lastly, a complex automotive scenario based on zonal architecture provided by a major motor company in Shanghai is analyzed in the simulated system; verifying TSN can guarantee real-time performance and reliability of the in-vehicle network.
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http://dx.doi.org/10.3390/s22124580DOI Listing
June 2022

LncRNA HAR1A Suppresses the Development of Non-Small Cell Lung Cancer by Inactivating the STAT3 Pathway.

Cancers (Basel) 2022 Jun 8;14(12). Epub 2022 Jun 8.

Department of Clinical Laboratory, Biobank, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin 150040, China.

It is imperative to advance the understanding of lung cancer biology. The Cancer Genome Atlas (TCGA) dataset was used for bioinformatics analysis. CCK-8 assay, flow cytometry, and western blot were performed in vitro, followed by in vivo study. We found that lncRNA () is significantly downregulated in lung adenocarcinoma (LUAD) and negatively associated with prognosis. We improved the prognostic accuracy of in LUAD by combining genes regulating cell apoptosis and cell cycle to generate a 23-gene signature. Nomogram and decision curve analysis (DCA) confirmed that the gene signature performed robustly in predicting overall survival. Gene set variation analysis (GSVA) demonstrated several significantly upregulated malignancy-related events in the high-risk group, including DNA replication, DNA repair, glycolysis, hypoxia, MYC targets v2, and mTORC1. The risk signature distinguished LUAD patients suitable for chemotherapies or targeted therapies. Additionally, the knockdown of accelerated NSCLC cell proliferation but inhibited apoptosis and vice versa. regulated cellular activities through the STAT3 signaling pathway. The tumor-suppressing role of was verified in the mouse model. Overall, the gene signature was robustly predictive of prognosis and sensitivity to anti-tumor drugs. functions as a tumor suppressor in NSCLC by regulating the STAT3 signaling pathway.
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http://dx.doi.org/10.3390/cancers14122845DOI Listing
June 2022

Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.

Gels 2022 Jun 15;8(6). Epub 2022 Jun 15.

Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, China.

Organoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not only recapitulate the histological and genetic characteristics of organs in vivo, but also form tissues with the capacity for self-renewal and further differentiation. Recent advances in biomaterial technology, particularly hydrogels, have provided opportunities to improve organoid cultures; by closely integrating the mechanical and chemical properties of the extracellular matrix microenvironment, with novel synthetic materials and stem cell biology. This systematic review critically examines recent advances in various strategies and techniques utilized for stem-cell-derived organoid culture, with particular emphasis on the application potential of hydrogel technology in organoid culture. We hope this will give a better understanding of organoid cultures for modelling diseases and tissue engineering applications.
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http://dx.doi.org/10.3390/gels8060379DOI Listing
June 2022

PPARγ Mediates Protective Effect against Hepatic Ischemia/Reperfusion Injury via NF-κB Pathway.

J Invest Surg 2022 Jun 22:1-12. Epub 2022 Jun 22.

Department of General Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.

Background: Hepatic ischemia/reperfusion injury (HIRI) is an unavoidable complication in liver surgery, however its pathological process is still unclear. Therefore, in this study, the role and mechanism of peroxisome proliferator-activated receptor gamma (PPARγ) was investigated in HIRI.

Materials And Methods: We constructed mice models with HIRI and L02 cell models insulted hypoxia/re-oxygenation (H/R). PPARγ agonist rosiglitazone was administered prior to HIRI in mice and PPARγ-siRNA was to H/R treatment in L02 cells. Liver injury was measured by serum ALT, AST and LDH levels and performing H&E staining; the inflammatory injury was reflected by inflammatory markers IL-1β, IL-6 and TNF-α, which were assayed by Real-time PCR and Western blotting, MPO activity was determined using commercial kits; oxidative stress injury was evaluated by iNOS, MDA, SOD and GSH-PX levels; apoptosis was detected by cleaved-Caspase-3, TUNEL staining and flow cytometry; NF-κB signaling activation was reflected by phosphorylation of IκBα (p-IκBα) and nuclear translocation of NF-κB p65.

Results: The level of PPARγ expression was obviously down-regulated both in mice liver subjected to IRI and in L02 cells to H/R. Overexpression of PPARγ presented protective effect on HIRI by reducing serum levels of aminotransferase and hepatic necrosis, inhibiting inflammation and apoptosis and alleviating oxidative stress in vivo. But PPARγ-siRNA aggravate H/R insult by promoting inflammation and apoptosis in vitro. Mechanistically, the NF-κB pathway activity was increased with PPARγ down-regulation by PPARγ-siRNA. Importantly, inhibition of NF-κB signaling abolished PPARγ knockdown-mediated hepatic injury.

Conclusions: PPARγ present protective effects on HIRI by attenuating liver injury, inflammatory response, oxidative stress and apoptosis in vivo and in vitro, and its mechanism may be related to down-regulation of NF-κB signaling.
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http://dx.doi.org/10.1080/08941939.2022.2090033DOI Listing
June 2022

Circular RNA hsa_circ_0000437 may be used as a new indicator for the diagnosis and prognosis of hepatocellular carcinoma.

Bioengineered 2022 Jun;13(6):14118-14124

Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangdong, Guangzhou, China.

Circular RNAs (circRNAs) play an essential role in hepatocellular carcinoma (HCC); however, the precise role of circRNAs in the diagnosis and prognosis of HCC remains unclear. The circRNA circ_0000437 was identified in the microarray dataset GSE166678 and was detected in HCC and paired adjacent tissue and serum samples in both the HCC and control groups by reverse transcription quantitative PCR. The association between circ_0000437 expression and clinicopathological characteristics was investigated. Furthermore, the diagnostic and prognostic values of circ_0000437 were determined using receiver operating characteristic (ROC) and Kaplan-Meier curves. Circ_0000437 expression was markedly upregulated in the tumor group compared with the control group and was correlated with tumor node metastasis (TNM) classification, differentiation degree, tumor size, and Barcelona Clinic Liver Cancer (BCLC) stage (< 0.05) in both the tumor tissues and serum. Furthermore, poor overall survival (OS) was correlated with high circ_0000437 expression, and the area under the ROC curve (AUC) of circ_0000437 for the diagnosis of HCC was 0.9281 in the serum. Our findings suggest that circ_0000437 may be used as a novel biomarker for the diagnosis and prognosis of patients with HCC.
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http://dx.doi.org/10.1080/21655979.2022.2081458DOI Listing
June 2022

Exploring the relationship of brown adipose tissue to bone microarchitecture using 7T MRI and micro-CT.

Histol Histopathol 2022 Jun 22:18481. Epub 2022 Jun 22.

Department of Radiology, The Third Hospital of Hebei Medical University, Hebei Province Biomechanical Key Laboratory of Orthopedics, Shijiazhuang, Hebei, China.

Background: Brown adipose tissue (BAT) is involved both in energy production and bone metabolism. The purpose of this study was to analyze the relationship between BAT and microarchitecture at cancellous and cortical bone using Kunming mice and the methods of 7T magnetic resonance imaging (MRI) combined with micro-CT.

Methods: Twenty-four female Kunming mice were examined by 7T MRI and measured T2* relaxation time on the deep and superficial interscapular BAT?iBAT?and subcutaneous white adipose tissue (sWAT). Cancellous bone microarchitecture of the distal femur and cortical bone of the middle femur were examined by micro-CT. A paired t-test was used to analyze the differences in T2* values between iBAT and sWAT. The correlation between BAT T2* values and bone microstructure parameters were analyzed using Pearson's correlation.

Results: T2* values of the deep and superficial iBAT (6.36±3.31 ms and 6.23±2.61 ms) were significantly shorter than those of sWAT (16.30±3.05 ms, tdeep iBAT=-10.816), tsuperficial iBAT =-12.276, p<0.01). Deep iBAT T2* values were significantly and negatively correlated with bone volume, cancellous thickness, and bone thickness (Th) and trabecular thickness (Tb.Th) of the cancellous bone of femur. Deep iBAT T2* values were significantly and positively correlated with the structural model index of cancellous bone of femur. Deep iBAT T2* values were significantly and negatively correlated with bone mineral density of the cortical bone of femur.

Conclusions: MRI can distinguish the two adipose tissues from each other. T2* values of BAT were lower than WAT on MRI. BAT related bone remodeling was more correlated with the microstructure of cancellous bone than that of cortical bone.
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http://dx.doi.org/10.14670/HH-18-481DOI Listing
June 2022

IQGAP1 is positively correlated with PD-L1 and regulates its expression via mediating STAT proteins phosphorylation.

Int Immunopharmacol 2022 Jul 31;108:108897. Epub 2022 May 31.

Department of Oncology, Wuxi Maternal and Child Health Hospital, the Affiliated Hospital to Nanjing Medical University, Wuxi 214023, China. Electronic address:

IQ motif containing GTPase activating protein 1 (IQGAP1) is a scaffold protein to transduce multiple signals from the extracellular microenvironment. However, whether IQGAP1 mediates EGF-induced cancer progression has been not observed. In this research, we explored the immunological correlations of IQGAP1. IQGAP1 was up-regulated in most cancer and related to poor prognosis in ovarian cancer. In addition, IQGAP1 was correlated with immune-related genes and tumor-infiltrating lymphocytes. IQGAP1 was a novel EGF-response gene, which was up-regulated by EGF. As the downstream of EGFR, IQGAP1 mediated EGF-induced proliferation, migration and invasion of tumor cells and was essential for EGF-induced PD-L1 expression as well. Mechanically, IQGAP1 interacted with STATs and mediated their phosphorylation. Furthermore, Curcumin inhibited EGFR/IQGAP1 axis to down-regulate PD-L1 and suppress cancer progression. To sum up, IQGAP1 responds to EGF stimulation and mediates EGF-induced phosphorylation of STATs, which is a novel promising target for blocking the EGF-induced cancer progression.
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http://dx.doi.org/10.1016/j.intimp.2022.108897DOI Listing
July 2022

Higher Dose of CD34 cells Promotes Early Reconstitution of Natural Killer Cells and Is Associated with Better Outcomes After Unmanipulated Hematopoietic Stem Cell Transplantation for Myeloid Malignancies.

Transplant Cell Ther 2022 Jun 14. Epub 2022 Jun 14.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300020, China.

Background: Natural killer (NK) cells are the first lymphocyte population to recover after allogenic hematopoietic stem cell transplantation (allo-HSCT) and mediate potent graft versus leukemia (GVL) effect particularly in the settings of T-cell depletion. However, the significance of NK cells after unmanipulated transplantation is less clear and factors affecting early NK reconstitution remain elusive.

Objectives And Study Design: We retrospectively analyze 180 patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) who received unmanipulated allografts. We focus on the early NK reconstitution and its association with disease relapse, overall survival and cytomegalovirus (CMV) reactivation. We also analyze factors that affect NK recovery for example dose of CD34 cells in the graft and T cell recovery after transplantation.

Results: Penalized splines and receiver operator characteristic (ROC) curves demonstrate a strong association between blood NK counts at 30 days after allo-HSCT (NK30) and all-cause mortality with the cut-off value being close to the median value which divides patients dichotomously. Subsequent analysis shows that rapid NK recovery (higher NK30 and/or higher NK60) is associated with reduced disease relapse and better survival. Robust NK recovery (NK30 and NK60) also correlates with lower incidence of CMV reactivation. We find that NK30 is associated with the numbers of CD34 cells (r = 0.739, P < 0.001) but not mature NK cells contained in the graft. In a small subset (N = 12) of the cohort, patients in continuous complete remission (N = 6) demonstrate higher frequencies of CD34CD7 progenitor cells and CD56 NK cells in the day +30 bone marrow as compared to patients with disease relapse within 1 year (N = 6). Furthermore, neither T cell recovery after transplantation nor application of anti-thymocyte globulins (ATG) in the conditioning regimen demonstrate suppressive effect on NK recovery.

Conclusion: Rapid NK cell recovery is associated with improved prognosis of unmanipulated transplantation for myeloid malignancies. Manipulation of NK cell recovery represents a feasible approach to improve transplant outcomes for example by optimizing CD34 cells in the graft.
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http://dx.doi.org/10.1016/j.jtct.2022.06.007DOI Listing
June 2022

Immune signatures of CD4 and CD68 predicts disease progression in cutaneous T cell lymphoma.

Am J Transl Res 2022 15;14(5):3037-3051. Epub 2022 May 15.

Department of Hematology, Peking University Shenzhen Hospital Shenzhen 518000, Guangdong, P. R. China.

Background: Cutaneous T-cell lymphoma (CTCL) is highly heterogeneous, and its prognosis is closely related to the disease stage. The tumor microenvironment (TME) is an important component of tumor tissue, driving cancer cell growth, progression, and metastasis. However, the diagnostic value of TME in CTCL has not yet been studied in-depth. To date, no study has performed a comprehensive evaluation of the significance of the TME in CTCL.

Methods: Using xCell methods based on bulk RNA sequencing data, we inferred immune cell fraction in the TME in 126 patients and assessed the prognostic importance of immune cells. Consensus clustering was performed to determine the TME subtypes and characterize the transcriptome of each subtype. Based on the TME subtypes, we established the disease progression model using random forest algorithms and logistic regression. The efficacy of the model was examined using an additional 49-patient cohort. Finally, we validated our finding at the protein level using immunochemistry in a 16-patient cohort.

Results: Patients with advanced CTCL presented with a more active immunity overall than those with early stage. Random forest algorithms revealed that the immune cells CD4, macrophages, and dendritic cells (DCs) were the most effective prognosis predictors. Therefore, we constructed a risk model using logistic regression based on these immune cells. The TME score could be used to effectively predict disease conditions in three datasets with the AUC of 0.9414, 0.7912, and 0.7665, respectively. Immunochemistry at the protein level revealed that helper T cells and the macrophage markers CD4 and CD68 could successfully distinguish different CTCL stages in patients, whereas the DC marker langerin showed no change with disease progression.

Conclusion: We found advanced-stage CTCL was associated with an active immune microenvironment, and the immune signatures CD4 and CD68 showed a relatively high accuracy in predicting CTCL disease progression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185059PMC
May 2022

Biomechanical analysis of vertebral wedge deformity in elderly women with quantitative CT-based finite element analysis.

BMC Musculoskelet Disord 2022 Jun 14;23(1):575. Epub 2022 Jun 14.

Department of Radiology, The Third Hospital of Hebei Medical University, No. 139 Ziqiang St, Qiaoxi District, Shijiazhuang, Hebei, CN 050050, China.

Background: To explore the vertebral deformity angle (VD angle) of 1 lumbar vertebral body (L1) in elderly women, investigate the influence of VD on vertebral stiffness (VS) by biomechanical analysis using quantitative computed tomography-based finite element analysis (QCT-FEA).

Methods: Two hundred seventy eight participants were recruited, and underwent QCT scan. Measured VD angles of L1, and constructed QCT-FEA models of L1 with the minimum (0.59°), median (5.79°) and maximum (11.15°) VD angles, respectively. Loads in two directions were applied on the upper edge of L1 with a force of 700 N, and vertebral stiffness (VS) was defined as the ratio of 700 N and displacement at the superior reference point: (1) perpendicular to the upper edge of L1 (defined as VS-U); (2) perpendicular to the lower edge of L1(defined as VS-L).

Results: Age was very weak positively correlated with VD angle, moderate negatively correlated with vBMD, and moderate negatively correlated with VS (P < 0.05). VS-U was significantly different among three VD angles, so was VS-L (P < 0.001). VS-U was higher than VS-L in 5.79° and 11.15° VD angles (P < 0.05), however no difference in 0.59° VD angles (P > 0.10).

Conclusions: VD angle of L1 was slightly increased with age and not correlated with vBMD, and VS was moderate negatively correlated with age, showing that the vertebral body was more likely to fracture with aging. VS-U and VS-L were gradually decreased with the increase of VD angle, and VS-L was lower than VS-U with the increase of VD angle, which showed that vertebral body was more prone to fracture when the load was perpendicular to the lower edge of the vertebral body as the VD angle increasing.
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http://dx.doi.org/10.1186/s12891-022-05518-zDOI Listing
June 2022

Itaconate alleviates β-microglobulin-induced cognitive impairment by enhancing the hippocampal amino-β-carboxymuconate-semialdehyde-decarboxylase/picolinic acid pathway.

Biochem Pharmacol 2022 Jun 11;202:115137. Epub 2022 Jun 11.

The First Affiliated Hospital, Institute of Neurology, Hengyang Medical School, University of South China, Hengyang 42100, Hunan, PR China; Hengyang Key Laboratory of Neurodegeneration and Cognitive Impairment, Institute of Neuroscience, Hengyang Medical School, University of South China, Hengyang 42100, Hunan, PR China. Electronic address:

β-microglobulin (BM) has been established to impair cognitive function. However, no treatment is currently available for BM-induced cognitive dysfunction. Itaconate is a tricarboxylic acid (TCA) cycle intermediate that exerts neuroprotective effects in several neurological diseases. The amino-β-carboxymuconate-semialdehyde-decarboxylase (ACMSD)/picolinic acid (PIC) pathway is a crucial neuroprotective branch in the kynurenine pathway (KP). The present study sought to investigate whether Itaconate attenuates BM-induced cognitive impairment and examine the mediatory role of the hippocampal ACMSD/PIC pathway. We demonstrated that 4-Octyl Itaconate (OI, an itaconate derivative) significantly alleviated BM-induced cognitive dysfunction and hippocampal neurogenesis impairment. OI treatment also increased the expression of ACMSD, elevated the concentration of PIC, and decreased the level of 3-HAA in the hippocampus of BM-exposed rats. Furthermore, inhibition of ACMSD by TES-991 significantly abolished the protections of Itaconate against BM-induced cognitive impairment and neurogenesis deficits. Exogenous PIC supplementation in hippocampus also improved cognitive performance and hippocampal neurogenesis in BM-exposed rats. These findings demonstrated that Itaconate alleviates BM-induced cognitive impairment by upregulation of the hippocampal ACMSD/PIC pathway. This is the first study to document Itaconate as a promising therapeutic agent to ameliorate cognitive impairment. Moreover, the mechanistic insights into the ACMSD/PIC pathway improve our understanding of it as a potential therapeutic target for neurological diseases beyond BM-associated neurocognitive disorders.
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http://dx.doi.org/10.1016/j.bcp.2022.115137DOI Listing
June 2022

Flocculation of Chlorella vulgaris-induced algal blooms: critical conditions and mechanisms.

Environ Sci Pollut Res Int 2022 Jun 14. Epub 2022 Jun 14.

College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, 550025, China.

Algal blooms have posed great threats to livestocks and human health. Although flocculation is effective, its efficiency may hinder the direct application for algal blooms. In this study, critical (optimal) conditions and mechanisms for AlCl, FeCl, poly-aluminum chloride (PAC), chitosan, and polydimethyldiallylammonium chloride (PDADMAC)-induced flocculation of Chlorella vulgaris (C. vulgaris) were studied. Results identified the critical conditions which can cause flocculation efficiencies over 90% in 45 min for the five flocculants. Specifically, 4~10-mg/L doses of PDADMAC were proved to be appropriate for the treatment of C. vulgaris-induced algal blooms at pH 6.0~12.0. To probe the underlying mechanisms, functional groups involved in flocculation, zeta potential, and species distribution were analyzed during flocculation. FT-IR results indicated that N-H stretching in amine and C-H deformation in aliphatics were involved in algal flocculation with FeCl, and C-H deformation played an important role with PDADMAC, PAC, and chitosan. For AlCl, zeta potential and species distribution results suggested that charge neutralization and adsorption bridging were responsible for algal flocculation at pH 6~8. However, adsorption bridging and sweeping effects were the main mechanisms at pH >3 for FeCl. The flocculation mechanisms for the rest of the three polymers were charge neutralization, adsorption bridging, and sweeping. Meanwhile, all the flocculation processes followed second-order kinetics. Strong linkages were found between the rate constant, fractal dimension, and flocculation efficiency (P < 0.05). The results of critical flocculation conditions and mechanisms indicated that PDADMAC was an excellent flocculant for C. vulgaris removing and recycling, especially in water bloom treatment.
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http://dx.doi.org/10.1007/s11356-022-21383-8DOI Listing
June 2022

Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway.

J Ethnopharmacol 2022 Sep 7;295:115449. Epub 2022 Jun 7.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing, 210023, China; State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Ethnopharmacological Relevance: Pinelliae Rhizoma Praeparatum (PRP) is a traditional processed product of Pinellia ternata (Thunb.) Berit., which mainly used for treating cold asthma (CA). However, the mechanism of action of PRP for treating CA have not been fully elucidated.

Aim Of The Study: To investigate the core active constituents and the pharmacological mechanism of PRP against CA.

Materials And Methods: Ovalbumin (OVA) and cold water-induced cold asthma model were established in male mice. The effects of water extract from PRP were evaluated by general morphological observation, expectorant activity, airway hyperresponsiveness, mucus hypersecretion, inflammatory cytokines, etc. Additionally, the mRNA and protein expression of mucin 5AC (MUC5AC) and aquaporin 5 (AQP5) in vivo and in vitro were detected by immunohistochemistry (IHC), qRT-PCR, and western blotting. The mechanisms of action were investigated through network pharmacology and transcriptomic, and validated through western blotting and molecular docking.

Results: PRP exhibited a favorable expectorant activity, and significantly reduced the airway inflammation, mucus secretion, and hyperresponsiveness in cold asthma model. It also reduced the levels of IL-4, IL-5, IL-8, and IL-13 in bronchoalveolar lavage fluid (BALF) and IL-4 and total IgE in serum, while obviously increased the levels of IL-10 and IFN-γ in serum for asthmatic mice. Meanwhile, PRP also attenuated the pathological changes and mucus production in cold asthmatic mice. Moreover, the downregulation of MUC5AC and upregulation of AQP 5 were detected by western blotting and qRT-PCR after administration with PRP both in vivo and in vitro. PRP expectedly inhibited the protein expression of PKC-α, SRC, p-EGFR, p-ERK1/2, p-JNK, p-p38, p-PI3K, and p-Akt levels in vivo.

Conclusions: These combined data showed that PRP suppressed the allergic airway inflammation of CA by regulating the balance of Th1 and Th2 cytokines and the possible involvement of the PKC/EGFR/MAPK/PI3K-Akt signaling pathway. Pentadecanoic acid, licochalcone A, β-sitosterol, etc. were considered as main active ingredients of PRP against CA. This study provides a novel perspective of the classical herbal processed product PRP in the treatment of CA.
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http://dx.doi.org/10.1016/j.jep.2022.115449DOI Listing
September 2022

Allantoin Inhibits Compound 48/80-Induced Pseudoallergic Reactions In Vitro and In Vivo.

Molecules 2022 May 27;27(11). Epub 2022 May 27.

Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.

Pseudoallergic reactions are hypersensitivity reactions mediated by an IgE-independent mechanism. Since allantoin (AT)-mediated pseudoallergy has not been studied, in this study, our objective is to investigate the anti-pseudoallergy effect of AT and its underlying mechanism. In vitro, β-hexosaminidase (β-Hex) and histamine (HIS) release assays, inflammatory cytokine assays, toluidine blue staining, and F-actin microfilament staining were used to evaluate the inhibitory effect of AT in RBL-2H3 cells stimulated with Compound 48/80 (C48/80). Western blot analysis is further performed to investigate intracellular calcium fluctuation-related signaling pathways. In vivo, Evans Blue extraction, paw swelling, and the diameter of Evans Blue extravasation were evaluated, and skin tissues are examined for histopathological examination in mice with passive cutaneous anaphylaxis (PCA) induced by C48/80. Body temperature is measured, and the levels of cytokines are further determined by ELISA kits in mice with active systemic anaphylaxis (ASA) induced by C48/80. The results show that AT dose-dependently inhibited degranulation in C48/80-stimulated RBL-2H3 cells by inhibiting β-Hex and HIS release, reducing the levels of TNF-α, IL-8, and MCP-1, inhibiting shape changes due to degranulation and disassembling the F-actin cytoskeleton. Furthermore, AT dose-dependently inhibits the phosphorylation of PLCγ and IP3R. In vivo, AT decreased Evans Blue extravasation, paw swelling, and the diameter of Evans Blue extravasation and significantly ameliorate pathological changes and mast cell degranulation in C48/80-induced PCA. Furthermore, AT help the mice recover from the C48/80-induced decrease in body temperature and decreased the levels of cytokines in C48/80-treated ASA mice. Our results indicate that allantoin inhibits compound 48/80-induced pseudoallergic reactions. AT has the potential to be used in IgE-independent anti-allergic and anti-inflammatory therapies.
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http://dx.doi.org/10.3390/molecules27113473DOI Listing
May 2022

Effect of Midwives' Application of Intelligent Delivery Room Management System on Delivery Outcome.

Comput Math Methods Med 2022 27;2022:4912053. Epub 2022 May 27.

Shijiazhuang Fourth Hospital, 16 Tanggu street, Chang'an District, Shijiazhuang City, Hebei Province, China.

Objective: To investigate the impact of midwives using an intelligent delivery room management system on the outcome of deliveries.

Method: A total of 100 primiparas admitted to the department of obstetrics and gynecology of our hospital from January 2019 to June 2020 were selected as the research objects. They were randomly assigned to one of two groups: control or observation. The control group got standard obstetric care. On the basis of the control group, midwives in the observation group applied the intelligent delivery room management system for delivery management. The outcomes of childbirth, postpartum anxiety, and postpartum depression were recorded and compared between the two groups.

Results: The observation group's first and second stages of labour were shorter than the control group's ( < 0.05), postpartum NRS score was lower than the control group's ( < 0.05), neonate Apgar score was higher than the control group's ( < 0.05), and the rate of vaginal delivery to caesarean section was lower than the control group's ( < 0.05). There was no statistical significance in prenatal S-AI scores between the observation group and the control group ( > 0.05). After delivery, the S-AI score of the observation group was lower than that of the control group, and the comparison result was statistically significant ( < 0.05). There was no significant difference in prenatal EPDS scores between the observation group and the control group ( > 0.05). After delivery, the EPDS score of the observation group was lower than that of the control group, and the comparison result was statistically significant ( < 0.05).

Conclusion: Midwives may employ sophisticated delivery room management technologies to improve birth outcomes and reduce maternal anxiety and depression, and it is something that should be extensively promoted in clinic.
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http://dx.doi.org/10.1155/2022/4912053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166941PMC
June 2022

Conditional knockout of Cdc20 attenuates osteogenesis in craniofacial bones.

Tissue Cell 2022 May 20;77:101829. Epub 2022 May 20.

Department of Prosthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, National Clinical Research Center for Oral Diseases, Beijing Key Laboratory of Digital Stomatology, Beijing, China. Electronic address:

Craniofacial bone defects cause significant problems to patients with harmful consequences. Mesenchymal stem cells (MSCs) can self-renew and exhibit multilineage differentiation, which could be applied to bone regeneration. However, craniofacial bone tissue MSCs have unique properties, differing in their characteristics to MSCs derived from long bones. CDC20 promotes osteogenic differentiation in long bones; however, its role in craniofacial bone tissues remains unknown. In this study, we found that Cdc20 conditional knockout in mice triggered distinctive cranial and mandibular bone loss. Moreover, the osteogenic differentiation potential of cranial suture-derived MSCs and mandibular bone marrow-derived MSCs was impaired in Cdc20 conditional knockout mice. The conditional knockout of Cdc20 impaired osteogenesis in craniofacial bones. Our findings provide new insights into craniofacial bone regeneration and the treatments of craniofacial bone-related diseases.
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http://dx.doi.org/10.1016/j.tice.2022.101829DOI Listing
May 2022

Radiotherapy-activated NBTXR3 nanoparticles modulate cancer cell immunogenicity and TCR repertoire.

Cancer Cell Int 2022 Jun 3;22(1):208. Epub 2022 Jun 3.

Nanobiotix, Paris, France.

Background: Radiotherapy is a powerful and widely used technique for the treatment of solid tumors. Beyond its ability to destroy tumor cells, it has been demonstrated that radiotherapy can stimulate the anti-tumor immune response. Unfortunately, this effect is mainly restricted to the irradiated lesion, as tumor control outside the treated field (called the 'abscopal effect') is rarely obtained. In addition, many pro-tumoral factors prevent this anti-tumor immune response from being sustained and efficient. We previously reported that radiotherapy-activated NBTXR3 produced a significant CD8-dependent abscopal effect in immunocompetent mice bearing CT26.WT tumors, while radiotherapy failed to generate such a response.

Methods: To identify the mechanisms that may explain this response, we evaluated the capacity of radiotherapy-activated NBTXR3 to modulate the immunogenicity of tumor cells by analysis of immunogenic cell death biomarkers and immunopeptidome sequencing. In vivo, we analyzed treated tumors for CD4+, CD8 + and CD68 + cell infiltrates by immunohistochemistry and digital pathology and sequenced the T cell receptor (TCR) repertoire in both treated and untreated distant tumors.

Results: We showed that NBTXR3 activated by radiotherapy both increased immunogenic cell death biomarkers and modulated the immunopeptidome profile of CT26.WT cells. Immunohistochemistry analysis of treated tumors revealed a significant increase in CD4+, CD8 + and CD68 + cell infiltrates for NBTXR3 activated by radiotherapy group, compared to radiotherapy. We also measured significant modifications in TCR repertoire diversity in the radiotherapy-activated NBTXR3 group, both in treated and distant untreated tumors, compared to radiotherapy alone.

Conclusions: These results indicate that radiotherapy-activated NBTXR3 can act as an effective immunomodulator, modifying tumor cell immunogenicity and impacting the lymphocyte population.
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http://dx.doi.org/10.1186/s12935-022-02615-wDOI Listing
June 2022

Percutaneous lumbopelvic fixation using modified iliac screw implantation: A technique description and case report.

Asian J Surg 2022 May 30. Epub 2022 May 30.

Department of Orthopedics, Zibo Municipal Hospital, Zibo, 255400, China.

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http://dx.doi.org/10.1016/j.asjsur.2022.05.057DOI Listing
May 2022

Childhood trauma and cognitive deficits in patients with schizophrenia: mediation by orbitofrontal cortex H-shaped sulci volume.

J Psychiatry Neurosci 2022 May-Jun;47(3):E209-E217. Epub 2022 Jun 2.

From the Peking University Huilongguan Clinical Medical School, Beijing Huilongguan Hospital, Beijing, P. R. China (L. Wang, Yin, Feng, Zhou, Huang, Zhang, Chen, Fan, S. Tan, Z. Wang, B. Tian, Y. Tan); the Department of Pharmacy, Peking University First Hospital, Beijing, P.R. China (Cui); the Department of Psychiatry, Yale University School of Medicine, New Haven, CT (Luo, Li); the Institute of Biomedicine and Translational Medicine, Department of Physiology, Faculty of Medicine, University of Tartu, Tartu, Estonia (L. Tian)

Background: A line of evidence has shown that childhood trauma and patterns of H-shaped sulci in the orbitofrontal cortex (OFC) are associated with cognitive deficits in patients with schizophrenia. Studies have also suggested that childhood trauma is associated with OFC volumetrics. This study investigated the interrelationship between childhood trauma, OFC H-shaped sulci volume and cognitive function in patients with first-episode schizophrenia. We hypothesized that OFC H-shaped sulci volume would mediate the relationship between childhood trauma and cognitive function in patients with first-episode schizophrenia.

Methods: We recruited patients with first-episode schizophrenia ( = 63) and healthy controls ( = 48), and quantified OFC H-shaped sulci volumes with 3.0 T high-resolution MRI. We assessed cognitive function and childhood trauma experiences using the MATRICS Consensus Cognitive Battery (MCCB) and the Childhood Trauma Questionnaire (CTQ).

Results: Patients with first-episode schizophrenia had smaller left OFC H-shaped sulci volumes, more severe childhood trauma experiences and worse cognitive function than healthy controls. CTQ total score and emotional and physical neglect subscores were negatively correlated with left OFC H-shaped sulci volume. CTQ total score and emotional neglect and sexual abuse subscores were negatively correlated with cognitive function in patients with first-episode schizophrenia. Interestingly, the CTQ total score and physical neglect subscore were positively correlated with cognitive function in healthy controls. Left OFC H-shaped sulci volume played a mediating role in CTQ emotional neglect subscore, CTQ total score and MCCB composite score.

Limitations: The small sample size and retrospective design need to be considered.

Conclusion: Childhood trauma might contribute to cognitive deficits in patients with first-episode schizophrenia by affecting left OFC H-shaped sulci volume. This finding can help in the design of strategies to improve cognitive function in patients with first-episode schizophrenia.
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http://dx.doi.org/10.1503/jpn.210178DOI Listing
June 2022

Mass Spectrometry-Based Quantitative Proteomics Analysis for Better Understanding of Telomeric Zinc Finger-Associated Protein-Induced Pathogenesis in Cardiomyocytes.

Int Heart J 2022 ;63(3):566-577

School of Clinical Medicine, Tsinghua University.

Telomere length is highly related to cardiovascular diseases. Telomeric zinc finger-associated protein (TZAP) directly binds to telomeric TTAGGG repeats via zinc finger domains and triggers the initiation of the telomere trimming process. However, proteomics analysis of TZAP in cardiomyocytes is slightly unknown. In our study, TZAP was overexpressed by adenovirus transfection in cultured H9c2 cardiomyocytes, and then mass spectrometry-based quantitative proteomics research strategies, including Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, subcellular localizations, predicted functional domains, and protein-protein interaction (PPI) analysis, were performed to explore TZAP-induced potential pathogenesis in cardiomyocytes. A total of 184 upregulated and 228 downregulated differentially expressed proteins (DEPs) were identified among identified 5693 quantifiable proteins in TZAP-overexpressed cardiomyocytes. These DEPs were mainly distributed in the nucleus, cytoplasm, and plasma membrane. DEPs were enriched in biological processes including cardiac muscle cell contraction, acute inflammatory response, cell-cell junction assembly, and macromolecule biosynthetic process. They were enriched in 9 KEGG pathways, including Hippo signaling pathway, protein digestion and absorption, and cytokine receptor interaction, and enriched in 17 protein domains, including translation initiation factor 1A/IF-1, class I histocompatibility antigen, and zinc finger. PPI analysis indicated that TZAP interacted with NDUFC2, Gja1, and HDAC2. Further, as proteins closely related to cardiovascular function, the mRNA levels of BRD4, Gja1, HDAC2, MAP2K3, Plakophilin 4, and Syndecan 1 significantly decreased, while Trpm7, clusterin, and NDUFC2 remarkably increased in TZAP-overexpressed cardiomyocytes by RT-PCR assay, which were consistent with the proteomics analysis. Collectively, we provided candidate proteins and enrichment pathways in TZAP-overexpressed cardiomyocytes, which need further investigation.
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http://dx.doi.org/10.1536/ihj.21-354DOI Listing
June 2022

Melamine self-assembly and dehydrogenation on Ag(111) studied by tip-enhanced Raman spectroscopy.

J Chem Phys 2022 May;156(20):204301

Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

The adsorption and self-assembly structures of melamine molecules on an Ag(111) surface are studied by low temperature scanning tunneling microscopy (STM) combined with tip-enhanced Raman spectroscopy (TERS). Two ordered self-assembly phases of melamine molecules on Ag(111) were studied by STM and TERS, combining with first-principles simulations. The α-phase consists of flat-lying melamine molecules, while the β-phase consists of mixed up-standing/tilted melamine molecules. Moreover, dehydrogenation of melamine can be controlled by annealing the sample as well as by a tip-enhanced photo-catalytic effect. Our work demonstrates TERS as a powerful tool not only for investigating the configuration and vibration properties of molecules on a metal surface with high spatial resolution but also for manipulating the chemical reactions with tip and photo-induced effects.
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http://dx.doi.org/10.1063/5.0091353DOI Listing
May 2022

Genetic susceptibility to high myopia in Han Chinese population.

Open Life Sci 2022 17;17(1):512-516. Epub 2022 May 17.

College of Life Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, 271016, China.

High myopia is a common ocular genetic disease in the world. The study sought to investigate the effect of the Insulin-like growth factor-1 (IGF-1) and Matrix metalloproteinase-9 (MMP-9) genes polymorphisms on high myopia in a Han population of China. This study recruited 216 unrelated Han Chinese subjects, including 103 cases with high myopia and 113 controls. Four tagging single nucleotide polymorphisms (SNPs) of and genes were genotyped using the Sequenom MassARRAY method. The chi-square test showed that the family history was significantly correlated with myopia. The SNP genotypes were all in Hardy-Weinberg equilibrium ( > 0.05). Among the four SNPs, there were statistically significant differences in the genotype and allele frequencies of between the groups ( = 0.024). The significant associations of between cases and controls also appeared after Bonferroni multiple correction ( = 0.024). Then, there were significant differences in the genotypes dominant model and codominant model of between groups ( = 0.007 and = 0.004, respectively). showed a significant difference between the cases and the controls in the recessive model ( = 0.037). Our findings indicated that of was associated with myopia in the population. The result suggested gene locus may play a role in myopia.
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http://dx.doi.org/10.1515/biol-2022-0055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123300PMC
May 2022

Surgery in platinum-resistant recurrent epithelial ovarian carcinoma.

World J Clin Cases 2022 Apr;10(12):3739-3753

Department of Gynecology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, Zhejiang Province, China.

Background: Ovarian cancer is one of the three most common malignant tumors of the female reproductive tract and ranks first in terms of mortality among gynecological tumors. Epithelial ovarian carcinoma (EOC) is the most common ovarian malignancy, accounting for 90% of all primary ovarian tumors. The clinical value of cytoreductive surgery in patients with platinum-resistant recurrent EOC remains largely unclear.

Aim: To evaluate the feasibility of secondary cytoreductive surgery for treating platinum-resistant recurrent EOC.

Methods: This was a retrospective study of the clinical data of patients with platinum-resistant EOC admitted to the Cancer Hospital of the University of Chinese Academy of Sciences between September 2012 and June 2018. Patient baseline data were obtained from clinical records. Routine follow-up of disease progression was performed as follows. CA125 assessment and physical examination were performed every 3 wk during treatment, including gynecological examination. Imaging assessment was carried out every 12 wk by B-mode ultrasound, computed tomography, or magnetic resonance imaging. The primary outcome was progression-free survival (PFS). Secondary outcomes included overall survival (OS), chemotherapy-free interval (CFI), and complications. Follow-up ended on April 15, 2019.

Results: A total of 38 patients were included. R0 resection was achieved in 25 (65.8%) patients and R1/2 in 13 (34.2%). Twenty-five (65.8%) patients required organ resection. Nine (23.7%) patients had operative complications, 36 (94.7%) received chemotherapy, and five (13.2%) had targeted therapy. Median PFS and OS were 10 (95%CI: 8.27-11.73) months and 28 (95%CI: 12.75-43.25) months, respectively; median CFI was 9 (95%CI: 8.06-9.94) months. R0 resection and postoperative chemotherapy significantly prolonged PFS and OS (all < 0.05), and R0 resection also significantly prolonged CFI ( < 0.05). Grade ≥ 3 complications were observed, including rectovaginal fistula ( = 1), intestinal and urinary fistulas ( = 1), and renal failure-associated death ( = 1). Except for the patient who died after surgery, all other patients with complications were successfully managed. Two patients developed intestinal obstruction and showed improvement after conservative treatment.

Conclusion: Secondary cytoreductive surgery is feasible for treating platinum-resistant recurrent EOC. These findings provide important references for the selection of clinical therapeutic regimens.
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http://dx.doi.org/10.12998/wjcc.v10.i12.3739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100723PMC
April 2022

Evaluation of C-shaped canals in maxillary molars in a Chinese population using CBCT.

BMC Med Imaging 2022 May 29;22(1):104. Epub 2022 May 29.

Department of Endodontics, Guiyang Hospital of Stomatology, Guiyang, 550002, Guizhou, China.

Introduction: The aim of this study was to evaluate the prevalence and characteristics of C-shaped root canals in maxillary first (MFMs) and second (MSMs) molars in a Southwestern Chinese population using cone-beam computed tomographic (CBCT).

Methods: CBCT images of MFMs (n = 1488) and MSMs (n = 1547) from 1508 subjects enrolled in Guiyang Hospital of Stomatology between July 2018 to February 2021 were evaluated for the incidence and types of C-shaped root canals. Differences by age, sex, left or right side, and bilateral symmetry were also evaluated.

Results: C-shaped root canals were present in 2.93% MFMs and MSMs (n = 3035) in the Southwestern Chinese population. A significant higher incidence was observed in the MSMs (5.24%) than in the MFMs (0.54%). Increased incidences were noted in teeth with fused root. Two major types and 5 subtypes of C-shaped canal system of maxillary molars were defined, and the most common type of C-shaped canals is fusion of mesial-buccal (MB) and distal-buccal (DB) canals (Type I subtype C). No significant gender and age differences were noted in the prevalence of C-shaped root canals in this population, and no significant difference was seen in its incidence in the left or right MFMs and MSMs. The bilateral occurrence was observed in 1.27% of the patients.

Conclusion: C-shaped root canals are more frequently present in MSMs than in MFMs in the Southwestern Chinese population, with Type I subtype C (MB-DB canal fusion) as the most common subtype and low incidence of bilateral symmetry.
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http://dx.doi.org/10.1186/s12880-022-00831-4DOI Listing
May 2022
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