Publications by authors named "Ping Xu"

1,786 Publications

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Unveiling the Coercivity-Induced Electrocatalytic Oxygen Evolution Activity of Single-Domain CoFeO Nanocrystals under a Magnetic Field.

J Phys Chem Lett 2022 Aug 8:7476-7482. Epub 2022 Aug 8.

MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Spin polarization modulation in ferromagnetic materials has become an effective way to promote the electrocatalytic oxygen evolution reaction (OER). Herein, to reveal the coercivity-related OER performance, single-domain ferromagnetic CoFeO (CFO) nanocrystals with different coercivities are synthesized and subjected to OER under an in situ tunable magnetic field. As the more ordered spin polarization state of CFO with a higher coercivity can afford a facilitated electron transfer process, the magnetic field-assisted OER activity can be more improved with an increase in coercivity. Specifically, the decreased magnitudes of the overpotential, Tafel slope, and charge transfer resistance increase on the samples with higher coercivity. The CFO with the largest coercivity (7500 Oe) shows the best OER performance with an overpotential of 350 mV at a current density of 10 mA cm under a magnetic field of 14000 G. In addition, a hysteresis effect that maintains enhanced OER current density after the magnetic field has been withdrawn is observed, where higher coercivity affords a longer hysteresis period. The exploration of coercivity-related OER enhancement may provide new insights into the design and synthesis of promising "magnetic effect" catalysts.
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http://dx.doi.org/10.1021/acs.jpclett.2c01843DOI Listing
August 2022

Two-dimensional chromatography in screening of bioactive components from natural products.

Phytochem Anal 2022 Aug 7. Epub 2022 Aug 7.

College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, China.

Introduction: Screening and analysis of bioactive components from natural products is a fundamental part of new drug development and innovation. Two-dimensional (2D) chromatography has been demonstrated to be an effective method for screening and preparation of specific bioactive components from complex natural products.

Objective: To collect details of application of 2D chromatography in screening of natural product bioactive components and to outline the research progress of different separation mechanisms and strategies.

Methodology: Three screening strategies based on 2D chromatography are reviewed, including traditional separation-based screening, bioactivity-guided screening and affinity chromatography-based screening. Meanwhile, in order to cover these aspects, selections of different separation mechanisms and modes are also presented.

Results: Compared with traditional one-dimensional (1D) chromatography, 2D chromatography has unique advantages in terms of peak capacity and resolution, and it is more effective for screening and identifying bioactive components of complex natural products.

Conclusion: Screening of natural bioactive components using 2D chromatography helps separation and analysis of complex samples with greater targeting and relevance, which is very important for development of innovative drug leads.
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http://dx.doi.org/10.1002/pca.3168DOI Listing
August 2022

Amniotic Membrane Enhances the Characteristics and Function of Stem Cell-Derived Retinal Pigment Epithelium Sheets by Inhibiting the Epithelial-Mesenchymal Transition.

Acta Biomater 2022 Aug 3. Epub 2022 Aug 3.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China. Electronic address:

Human pluripotent stem cell-derived retinal pigment epithelium (iRPE) is an attractive cell source for disease modeling and cell replacement therapy of retinal disorders with RPE defects. However, there are still challenges to develop appropriate culture conditions close to in vivo microenvironment to generate iRPE sheets, which mimic more faithfully the characteristics and functions of the human RPE cells. Here, we developed a simple, novel platform to construct authentic iRPE sheets using human amniotic membrane (hAM) as a natural scaffold. The decellularized hAM (dAM) provided a Bruch's membrane (BM)-like bioscaffold, supported the iRPE growth and enhanced the epithelial features, polarity distribution and functional features of iRPE cells. Importantly, RNA-seq analysis was performed to compare the transcriptomes of iRPE cells cultured on different substrates, which revealed the potential mechanism that dAM supported and promoted iRPE growth was the inhibition of epithelial mesenchymal transition (EMT). The tissue-engineered iRPE sheets survived and kept monolayer when transplanted into the subretinal space of rabbits. All together, our results indicate that the dAM imitating the natural BM allows for engineering authentic human RPE sheets, which will provide valuable biomaterials for disease modeling, drug screening and cell replacement therapy of retinal degenerative diseases. STATEMENT OF SIGNIFICANCE: : Engineered RPE sheets have a great advantage over RPE cell suspension for transplantation as they support RPE growth in an intact monolayer which RPE functions are dependent on. The substrates for RPE culture play a critical role to maintain the physiological functions of the RPE in stem cell therapies for patients with retinal degeneration. In this study, we constructed engineered iRPE sheets on the decellularized human amniotic membrane (dAM) scaffolds, which contributed to enhancing epithelial features, polarity distribution and functional features of iRPE. dAM exhibited the ability of anti-epithelial mesenchymal transition (EMT) to support iRPE growth. Furtherly, the results of transplanted in vivo demonstrated the feasibility of iRPE sheets in retina regenerative therapy. Engineering RPE sheets on dAM is a promising strategy to facilitate the development of iRPE replacement therapy and retinal disease modeling.
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http://dx.doi.org/10.1016/j.actbio.2022.07.064DOI Listing
August 2022

Phosphoproteome profiling of mouse liver during normal aging.

Proteome Sci 2022 Aug 5;20(1):12. Epub 2022 Aug 5.

State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China.

Background: Aging is a complex biological process accompanied by a time-dependent functional decline that affects most living organisms. Omics studies help to comprehensively understand the mechanism of aging and discover potential intervention methods. Old mice are frequently obese with a fatty liver.

Methods: We applied mass spectrometry-based phosphoproteomics to obtain a global phosphorylation profile of the liver in mice aged 2 or 18 months. MaxQuant was used for quantitative analysis and PCA was used for unsupervised clustering.

Results: Through phosphoproteome analysis, a total of 5,685 phosphosites in 2,335 proteins were filtered for quantitative analysis. PCA analysis of both the phosphoproteome and transcriptome data could distinguish young and old mice. However, from kinase prediction, kinase-substrate interaction analysis, and KEGG functional enrichment analysis done with phosphoproteome data, we observed high phosphorylation of fatty acid biosynthesis, β-oxidation, and potential secretory processes, together with low phosphorylation of the Egfr-Sos1-Araf/Braf-Map2k1-Mapk1 pathway and Ctnnb1 during aging. Proteins with differentially expressed phosphosites seemed more directly related to the aging-associated fatty liver phenotype than the differentially expressed transcripts. The phosphoproteome may reveal distinctive biological functions that are lost in the transcriptome.

Conclusions: In summary, we constructed a phosphorylation-associated network in the mouse liver during normal aging, which may help to discover novel antiaging strategies.
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http://dx.doi.org/10.1186/s12953-022-00194-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354360PMC
August 2022

Electronegativity Enhanced Strong Metal-Support Interaction in [email protected] for Enhanced Alkaline Hydrogen Evolution.

ACS Appl Mater Interfaces 2022 Aug 5. Epub 2022 Aug 5.

School of Physics, Harbin Institute of Technology, Harbin 150001, China.

Precious metals (Pt, Ir, Ru, and so on) and related compounds usually demonstrate superb catalytic activity for electrochemical hydrogen production. However, scarcity and stability are still challenges for hydrogen evolution reaction, even for single-atomic-site electrocatalysts. Herein, a fluorine (F) doping strategy is proposed to enhance the strong metal-support interaction between the F-doped NiN support and the loaded ruthenium (Ru) species. Via synergistically modulating both the Ru loading amount and F doping concentration, outstanding HER activity was achieved in [email protected] with an overpotential (η) of 115 mV at 100 mA cm, superior to the benchmark Pt/C (η = 201 mV). Density functional theory simulation in combination with X-ray photoelectron spectra and X-ray absorption spectroscopy characterizations convincingly demonstrate that, with the strongest electronegativity, F doping could effectively stabilize Ru atoms doped in the F-NiN substrate and simultaneously reduce the H bonding strength, which accelerated the desorption of H. These findings provide a facile strategy to modulate both catalytic activities and stabilities of heteroatom-loaded catalytic materials.
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http://dx.doi.org/10.1021/acsami.2c09507DOI Listing
August 2022

Clinical and Genetic Analysis of Retinitis Pigmentosa with Primary Angle Closure Glaucoma in the Chinese Population.

Curr Eye Res 2022 Aug 3:1-7. Epub 2022 Aug 3.

Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Purpose: Retinitis pigmentosa (RP) constitutes a class of common inherited retinal dystrophies. Patients with RP and comorbid primary angle-closure glaucoma (PACG) have been described, but the relationship between the diseases remains unclear. This study investigated the clinical and genetic characteristics of Chinese patients with RP and comorbid PACG.

Methods: Of 1356 patients with RP, we analyzed the genetic features of 39 RP patients with PACG using next-generation sequencing and reviewed their clinical characteristics.

Results: In total, 18 patients with acute PACG and 21 patients with chronic PACG were included in this study; their age at examination was 50.54 ± 12.99 years (range, 25.0-71.0 years), and their age at PACG onset was 46.04 ± 14.50 years (range, 24.9-68.0 years). Additionally, the mean lens thickness (LT) was 4.49 ± 0.44 μm, and the mean axial length (AL) was 22.63 ± 1.17 mm. Notably, the prevalence of PACG in patients with RP was 2.88%; this was higher than the prevalence in the general population. This could be explained by nanophthalmos, thickened lentis, ectopia lentis, or zonular insufficiency. Furthermore, patients with a shorter AL, a greater LT, iridociliary cysts, or nanophthalmos exhibited earlier development of PACG. Overall, 30 disease-causing variants spanning 17 genes were identified in 56.41% of the patients, and was the most common mutation gene.

Conclusions: Our findings revealed that there is a strong association between RP and PACG. Furthermore, intraocular pressure (IOP) should be measured in patients with RP to protect them from the aggravated damage of an elevated IOP.
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http://dx.doi.org/10.1080/02713683.2022.2085303DOI Listing
August 2022

MOFs-Derived Flower-Like Hierarchically Porous Zn-Mn-Se/C Composite for Extraordinary Rate Performance and Durable Anode of Sodium-Ion and Potassium-Ion Batteries.

Small 2022 Jul 31:e2203964. Epub 2022 Jul 31.

National Key Laboratory of Science and Technology for National Defence on High-strength Structural Materials, Central South University, Changsha, 410083, P. R. China.

The slow kinetics and poor structural stability prevent transition metal selenides from being widely used in sodium-ion batteries (SIBs) and potassium-ion batteries (PIBs). Herein, the "flower-like" porous carbon anchored by Zn-Mn binary selenides ([email protected]) composites are fabricated by selenizing the modified hierarchically metal-organic frameworks. The 2D conductive hierarchically flakes' abundant pore structure and multiple active sites shorten the ion diffusion length and promote conductivity, while the synergistic effect of the binary metals and intrinsic large pseudocapacitive contribution effectively improve capacity and rate performance. [email protected] composites exhibit impressive rate capability of 294.4 mA h g at 10 A g and excellent cyclic stability with 369.6 mA h g specific capacity retention at 2 A g after 1000 cycling in SIBs. It is noted that 156.9 mA h g can be retained at 5 A g and 227.0 mA h g is remained after 500 cycles at 2 A g in PIBs. The ex situ X-ray diffraction patterns and transmission electron microscopy pictures are used to confirm the conversion reaction processes of the Zn-Mn-Se. Designing high-performance energy storage materials may benefit greatly from the universal synthesis technology of bimetallic sulfide anodes for enhanced SIBs and PIBs.
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http://dx.doi.org/10.1002/smll.202203964DOI Listing
July 2022

Evaluation of nursing students' efficacy, attitude, and confidence level in a perioperative setting using virtual-reality simulation.

Nurs Forum 2022 Jul 31. Epub 2022 Jul 31.

Department of Surgery, National University Hospital, Singapore City, Singapore.

Aims: This study aimed to evaluate the efficacy, attitude, and level of confidence among nursing students as scrub nurses in a perioperative environment via virtual-reality simulation.

Background: The advantages of virtual reality have been demonstrated to provide a safe and effective mechanism to educate and evaluate students' performances, but the use of virtual reality in perioperative nursing remained limited.

Methods: Nineteen sessions were conducted in a room equipped with virtual-reality headsets and two touch controllers. The surgical workflow of a scrub nurse was developed based on qualitative perspectives from nursing faculties and hospital clinicians working in operating theaters. A survey on the efficacy, attitude, and level of confidence among the participants was conducted after the virtual-reality simulation.

Results: Two hundred and seven nursing students due for their clinical posting in operating theaters completed the evaluation. More than 90% of them indicated "neutral" and above in terms of efficacy, attitude, and confidence level with Cronbach's α values exceeding .90.

Conclusions: Virtual reality offers promising potential as an alternative clinical experience to physical simulation for nursing students to increase their efficacy, attitude, and level of confidence as scrub nurses in a perioperative environment through a standardized scenario-based digital environment.
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http://dx.doi.org/10.1111/nuf.12783DOI Listing
July 2022

The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas.

J Ovarian Res 2022 Jul 30;15(1):89. Epub 2022 Jul 30.

Department of Gynaecology, Women's Hospital, School of Medicine, Zhejiang University, 310006, Hangzhou, P.R. China.

Background: Many factors can affect the recurrence of endometriosis after surgery, however, whether endometriotic cyst fluid contributes to endometriosis recurrence after surgical excision of ovarian endometriomas remains unclear. The objective of this study was to determine the presence of endometrial cells in ovarian endometriosis cyst fluid and the potential differences between these cells and those in the cyst wall.

Methods: Samples of cyst fluid (n = 39) and drainage fluid (n = 14) were collected from patients with ovarian endometriomas undergoing laparoscopic surgery. Drainage fluid from 14 patients without endometriosis was used as a control. The presence of endometrial cells in cyst fluid and drainage fluid was determined by cell culture in vitro and immunostaining. In addition, cyst fluid endometrial fragments and viscosity were analysed by transcriptome sequencing analysis and apparent diffusion coefficients, respectively. An animal model was used to confirm the ability of endometrial cells in cyst fluid to form new lesions.

Results: We found endometrium-like tissues in 71.8% (28/39) of cyst fluid and 71.4% (10/14) of drainage fluid samples by histopathological examination, and the presence of endometrioid tissue in cyst fluid was related to the viscosity of the cyst fluid. The living endometrial cells in cyst fluid and drainage fluid were confirmed by cell culture in vitro and immunostaining. Moreover, the adhesion ability of endometrial fragments in cyst fluid was significantly higher than that of ectopic tissues in the cyst wall (P < 0.05). In addition, living endometrial cells in the cyst fluid were able to adhere and alive in the animal model.

Conclusions: The existence of living endometrial cells with high adhesion ability in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas due to cyst fluid outflow during the surgical procedure.
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http://dx.doi.org/10.1186/s13048-022-01018-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338681PMC
July 2022

Distribution and clinical significance of circulating CD8CD28 regulatory T cells in the peripheral blood of patients with pulmonary tuberculosis.

BMC Pulm Med 2022 Jul 30;22(1):291. Epub 2022 Jul 30.

Department of Clinical Laboratory, The Fifth People's Hospital of Suzhou, 10, Guanqian Road, Suzhou, 215000, Jiangsu, People's Republic of China.

Background: Regulatory T cells (Treg cells) in the peripheral blood of patients with pulmonary tuberculosis (PTB) may be closely related to the progression of PTB. In this study, the distribution characteristics and clinical importance of CD8CD28 Treg cells in patients with tuberculosis were systematically analyzed, and the role and importance of CD8CD28 Treg cells in influencing the immune response and progression of tuberculosis were discussed, which will provide immunological indices and reference values for the clinical diagnosis of tuberculosis.

Methods: Flow cytometry, sputum smears and computed tomography imaging were used to analyze the distribution characteristics of CD8CD28 Treg cells in the peripheral blood of patients with PTB and the correlation between CD8CD28Treg cells and clinical and immune indices.

Results: The percentages of CD4CD25 and CD8CD28 Treg cells in the peripheral blood of patients with PTB were significantly higher than those in the healthy control (HC) group. Further analysis showed that the percentage of CD4CD25Treg cells in the Stage II group was significantly higher than that in the HC group. The percentages of CD4CD25 and CD8CD28 Treg cells increased significantly in patients in the Stage II group. The proportion of CD8CD28 Treg cells was directly proportional to the degree of positivity in sputum smears, while CD4CD25Treg cells did not exhibit this trend. The correlations between the percentage of CD4CD25 and CD8CD28 Treg cells and the percentage of lymphocyte subsets were examined. The percentage of CD8CD28 Treg cells was negatively correlated with the percentage of CD4T cells and positively correlated with the CD8T cell percentage in the HC and PTB groups. The percentage of CD4 + CD25Treg cells was positively correlated with the percentage of CD4T cells only in the PTB group.

Conclusions: This study was the first to show that the proportion of CD8CD28 Treg cells in the peripheral blood of patients with PTB was significantly increased, and the increase in CD8CD28 Treg cells was related to the progression of PTB, which may affect the proportion of immune cell subsets by inhibiting the immune response, resulting in the progression of PTB.
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http://dx.doi.org/10.1186/s12890-022-02088-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338636PMC
July 2022

Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-Growing .

Front Bioeng Biotechnol 2022 12;10:955097. Epub 2022 Jul 12.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Sustainable and environment-friendly microbial fermentation processes have been developed to produce numerous chemicals. However, the high energy input required for sterilization and substantial fresh water consumption restrict the economic feasibility of traditional fermentation processes. To address these problems, , a promising microbial chassis with low nutritional requirements, high salt tolerance and rapid growth rate can be selected as the host for chemical production. In this study, was metabolic engineered to produce 2,3-butanediol (2,3-BD), an important platform chemical, through non-sterilized fermentation with seawater-based minimal medium after expressing a 2,3-BD synthesis cluster and deleting two byproduct encoding genes. Under optimized fermentative conditions, 41.27 g/L 2,3-BD was produced with a productivity of 3.44 g/L/h and a yield of 0.39 g/g glucose by recombinant strain ΔΔ-pETRABC. This study confirmed the feasibility of non-sterilized fermentation using seawater to replace freshwater and other valuable chemicals may also be produced through metabolic engineering of the emerging synthetic biology chassis .
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http://dx.doi.org/10.3389/fbioe.2022.955097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315368PMC
July 2022

From Rate-Limiting Enzyme to Therapeutic Target: The Promise of NAMPT in Neurodegenerative Diseases.

Front Pharmacol 2022 12;13:920113. Epub 2022 Jul 12.

Innovation Center of Nursing Research, West China School of Nursing, Department of Gastrointestinal Surgery, National Clinical Research Center for Geriatrics, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University, Chengdu, China.

Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD) salvage pathway in mammals. It is of great significance in the metabolic homeostasis and cell survival via synthesizing nicotinamide mononucleotide (NMN) through enzymatic activities, serving as a key protein involved in the host's defense mechanism. The NAMPT metabolic pathway connects NAD-dependent sirtuin (SIRT) signaling, constituting the NAMPT-NAD-SIRT cascade, which is validated as a strong intrinsic defense system. Neurodegenerative diseases belong to the central nervous system (CNS) disease that seriously endangers human health. The World Health Organization (WHO) proposed that neurodegenerative diseases will become the second leading cause of human death in the next two decades. However, effective drugs for neurodegenerative diseases are scant. NAMPT is specifically highly expressed in the hippocampus, which mediates cell self-renewal and proliferation and oligodendrocyte synthesis by inducing the biosynthesis of NAD in neural stem cells/progenitor cells. Owing to the active biological function of NAMPT in neurogenesis, targeting NAMPT may be a powerful therapeutic strategy for neurodegenerative diseases. This study aims to review the structure and biological functions, the correlation with neurodegenerative diseases, and treatment advance of NAMPT, aiming to provide a novel idea for targeted therapy of neurodegenerative diseases.
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http://dx.doi.org/10.3389/fphar.2022.920113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322656PMC
July 2022

Simultaneous quantification of multiple amino acid adducts from sulfur mustard-modified human serum albumin in plasma at trace exposure levels by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry after propionyl derivatization.

J Chromatogr A 2022 Aug 19;1678:463354. Epub 2022 Jul 19.

State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China. Electronic address:

Sulfur mustard (HD) is a highly toxic vesicant and is prohibited by the Organisation for the Prohibition of Chemical Weapons (OPCW). HD can modify human serum albumin (HSA) to generate hydroxyethylthioethyl (HETE) adducts, which could be utilized as biomarkers for verifying HD exposure in forensic analysis. Here, five amino acid adducts generated from pronase digestion of HD-exposed human serum albumin (HD-HSA) in plasma were selected as biomarkers to retrospectively detect HD exposure. HD-HSA was precipitated from plasma with acetone, digested by pronase, derivatized with propionic anhydride (PA), and analysed with ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-TQ MS). The limits of detection (LODs) and limits of quantification (LOQs) of the HD exposure concentrations were evaluated as 1.00 ng/mL at S/N≥3 and 3.00 ng/mL at S/N≥10, respectively, which are approximately 60 times lower than those of the reported method. The approach shows good linearity (R≥0.997) from 3.00 ng/mL to 10.0 µg/mL of HD-exposed human plasma with satisfactory precision and accuracy. The developed approach was applied to analysing samples from the 6 OPCW Biomedical Proficiency Test (BioPT). The study showed that the developed approach was also suitable for analysing human plasma samples that were exposed to six of HD analogues, which were common impurities in sulfur mustard mixtures. Moreover, the method was successfully applied to plasma from other species, including rabbits, rats and cattle. This study provides a reliable and sensitive tool for the retrospective detection of vesicants exposure based on multiple biomarkers.
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http://dx.doi.org/10.1016/j.chroma.2022.463354DOI Listing
August 2022

Motion and Load Analysis of the Flexible Platform Based on Noncontact Processing.

Micromachines (Basel) 2022 Jun 24;13(7). Epub 2022 Jun 24.

State Key Laboratory of Mechanical Transmission, Chongqing University, Chongqing 400030, China.

In this paper, we explore the applicability of the positioning stage based on flexible hinges for noncontact processing. According to the actual application of the positioning stage, Hooke's law, the Euler-Bernoulli beam theory, and the geometric relationship of the structure are applied to analyze the coupled displacement in the movement of the positioning stage and the changes in the performance of the positioning stage caused by external loads. The coupled-displacement matrix and the external-load matrix obtained from the analysis are substituted into the ideal-displacement expression of the positioning stage to obtain the displacement expression of the platform in noncontact machining. The platform trajectory obtained by the referenced curve is analyzed. In addition, the coupled displacement in the X- and Y-directions and the coupled displacement caused by the external load in the Z-direction are nanoscales and about one-thousandth of the output displacement, which meets the requirement of tracking accuracy for micron-level machining. Finally, we use finite element analysis (FEA) and experiments to prove the correctness of the theoretical analysis.
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http://dx.doi.org/10.3390/mi13070988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317106PMC
June 2022

Associations between Circulating SELENOP Level and Disorders of Glucose and Lipid Metabolism: A Meta-Analysis.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.

Selenoprotein P (SELENOP) is an extracellular antioxidant, selenium transporter, and hepatokine interfering with glucose and lipid metabolism. To study the association between the circulating SELENOP concentration and glucose and lipid metabolic diseases (GLMDs), including gestational diabetes (GD), metabolic syndrome (MetS), non-alcoholic fatty liver disease, obesity, and type 2 diabetes, as well as the individual markers, a meta-analysis was conducted by searching multiple databases from their establishment through March 2022 and including 27 articles published between October 2010 and May 2021, involving 4033 participants. Participants with GLMDs had higher levels of SELENOP than those without GLMDs (standardized mean difference = 0.84, 95% CI: 0.16 to 1.51), and the SELENOP levels were positively correlated with the markers of GLMDs (pooled effect size = 0.09, 95% CI: 0.02 to 0.15). Subgroup analyses showed that the SELENOP concentrations were higher in women with GD and lower in individuals with MetS than their counterparts, respectively. Moreover, SELENOP was positively correlated with low-density lipoprotein cholesterol, but not with the other markers of GLMDs. Thus, the heterogenicity derived from diseases or disease markers should be carefully considered while interpreting the overall positive association between SELENOP and GLMDs. Studies with a larger sample size and advanced design are warranted to confirm these findings.
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http://dx.doi.org/10.3390/antiox11071263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311835PMC
June 2022

Host genetic determinants drive compartment-specific assembly of tea plant microbiomes.

Plant Biotechnol J 2022 Jul 25. Epub 2022 Jul 25.

Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.

Diverse host factors drive microbial variation in plant-associated environments, whereas their genetic mechanisms remain largely unexplored. To address this, we coupled the analyses of plant genetics and microbiomes in this study. Using 100 tea plant (Camellia sinensis) cultivars, the microbiomes of rhizosphere, root endosphere and phyllosphere showed clear compartment-specific assembly, whereas the subpopulation differentiation of tea cultivars exhibited small effects on microbial variation in each compartment. Through microbiome genome-wide association studies, we examined the interactions between tea genetic loci and microbial variation. Notably, genes related to the cell wall and carbon catabolism were heavily linked to root endosphere microbial composition, whereas genes related to the metabolism of metal ions and small organic molecules were overrepresented in association with rhizosphere microbial composition. Moreover, a set of tea genetic variants, including the cytoskeleton-related formin homology interacting protein 1 gene, were strongly associated with the β-diversity of phyllosphere microbiomes, implying their interactions with the overall structure of microbial communities. Our results create a catalogue of tea genetic determinants interacting with microbiomes and reveal the compartment-specific microbiome assembly driven by host genetics.
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http://dx.doi.org/10.1111/pbi.13897DOI Listing
July 2022

Inhibition of STAT3 signal pathway recovers postsynaptic plasticity to improve cognitive impairment caused by chronic intermittent hypoxia.

Sleep Breath 2022 Jul 23. Epub 2022 Jul 23.

Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi Medical University, 149 Dalian Road, Zunyi, Guizhou, 563003, China.

Purpose: Chronic intermittent hypoxia (CIH) is a major cause of cognitive dysfunction in people with obstructive sleep apnea syndrome (OSAS), as it damages synapse structure, and function. This study aimed to investigate the potential mechanisms resulting in cognitive impairment caused by CIH in patients with OSAS.

Methods: Healthy adult SD male rats (n = 36) were randomly divided into four groups: control, CIH, WP1066, and dimethyl sulfoxide (DMSO). The CIH, WP1066, and DMSO groups were exposed to intermittent hypoxic environments for 8 h per day for 28 d. The WP1066 group received intraperitoneal injection of WP1066, a selective signal transducer and activator of transcription-3 (STAT3) inhibitor. All the experimental rats were subjected to the Morris water maze. Hippocampal tissue samples (n = 6 per group) were used for western blot analysis, and brain tissue samples (n = 3 per group) were used for immunohistochemistry and hematoxylin and eosin staining.

Results: The cognition of rats exposed to prolonged CIH was impaired. P-STAT3 expression was found to be higher in CIH rats than in control rats. Postsynaptic density95 (PSD95) expression was significantly reduced in rats with CIH-induced learning and memory impairment, but it significantly increased after the STAT3 signaling pathway was blocked, which improved learning and memory ability. However, inhibition of the STAT3 signaling pathway failed to improve the decline of synaptophysin (SYP) protein caused by CIH.

Conclusions: When rats are exposed to CIH, STAT3 in the brain is activated, PSD95 and SYP levels decrease, and cognition is impaired. Inhibition of the STAT3 signaling pathway increases PSD95 to recover postsynaptic plasticity, thereby improving cognitive dysfunction.
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http://dx.doi.org/10.1007/s11325-022-02671-6DOI Listing
July 2022

Association of Serum Apolipoprotein A5 Concentration with Nonalcoholic Fatty Liver Disease in Ningbo, China.

Contrast Media Mol Imaging 2022 8;2022:7015528. Epub 2022 Jul 8.

Department of Gastroenterology, The Ningbo Seventh Hospital, Ningbo 315200, China.

Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease characterized by the excessive accumulation of hepatocyte fat and steatosis in the absence of alcohol or any other clear contributing factors to liver injury. NAFLD has been confirmed to be closely associated with obesity, insulin resistance, and dyslipidemia. Genetic polymorphism studies have shown the relations between the apolipoprotein A5 gene (APOA5) and NAFLD. However, the association between the serum ApoA5 level and NAFLD remains unclear. Between September 2018 and August 2019, adults who attended the hospital-based health checkup center were enrolled in this study. Anthropometric examination, laboratory investigations on fasting blood, and abdominal ultrasonography were performed. The serum ApoA5 level was determined by enzyme-linked immunosorbent assay. A total of 517 eligible participants (317 females and 200 males) were involved in this study, with a mean age of 54.7 ± 16.7 years. The mean ApoA5 concentration was 28.8 ± 4.7 g/ml, among which the males had higher concentration levels than females (29.3 ± 4.5 vs. 28.5 ± 4.7 g/mL, =0.04). Serum ApoA5 level was not significantly correlated with NAFLD or metabolic profiles. However, the prevalence rate of hypertriglyceridemia (triglyceride ≥ 1.7 mmol/L) showed a significant inverted "U"-shaped trend in individuals with the serum ApoA5 level of quartile one to quartile four after adjusting the confounding factors. Moreover, individuals with higher serum ApoA5 levels were also more likely to suffer from hyperglycemia. The ApoA5 levels and the prevalence of hypertriglyceridemia are in an inverted "U-shaped" correlation, but there is no significant difference between ApoA5 levels, NAFLD, and metabolic syndrome.
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http://dx.doi.org/10.1155/2022/7015528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286930PMC
July 2022

Effectiveness of automated alerting system compared to usual care for the management of sepsis.

NPJ Digit Med 2022 Jul 19;5(1):101. Epub 2022 Jul 19.

Department of Emergency Medicine, Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

There is a large body of evidence showing that delayed initiation of sepsis bundle is associated with adverse clinical outcomes in patients with sepsis. However, it is controversial whether electronic automated alerts can help improve clinical outcomes of sepsis. Electronic databases are searched from inception to December 2021 for comparative effectiveness studies comparing automated alerts versus usual care for the management of sepsis. A total of 36 studies are eligible for analysis, including 6 randomized controlled trials and 30 non-randomized studies. There is significant heterogeneity in these studies concerning the study setting, design, and alerting methods. The Bayesian meta-analysis by using pooled effects of non-randomized studies as priors shows a beneficial effect of the alerting system (relative risk [RR]: 0.71; 95% credible interval: 0.62 to 0.81) in reducing mortality. The automated alerting system shows less beneficial effects in the intensive care unit (RR: 0.90; 95% CI: 0.73-1.11) than that in the emergency department (RR: 0.68; 95% CI: 0.51-0.90) and ward (RR: 0.71; 95% CI: 0.61-0.82). Furthermore, machine learning-based prediction methods can reduce mortality by a larger magnitude (RR: 0.56; 95% CI: 0.39-0.80) than rule-based methods (RR: 0.73; 95% CI: 0.63-0.85). The study shows a statistically significant beneficial effect of using the automated alerting system in the management of sepsis. Interestingly, machine learning monitoring systems coupled with better early interventions show promise, especially for patients outside of the intensive care unit.
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http://dx.doi.org/10.1038/s41746-022-00650-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296632PMC
July 2022

CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.

Int J Biol Sci 2022 27;18(10):4171-4186. Epub 2022 Jun 27.

Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, 510315 Guangzhou, China.

CCDC65 is a member of the coiled-coil domain-containing protein family and was only reported in gastric cancer by our group. We first observed that it is downregulated in lung adenocarcinoma based on the TCGA database. Reduced CCDC65 protein was shown as an unfavorable factor promoting the clinical progression in lung adenocarcinoma. Subsequently, CCDC65 mice were found possibly dead of hydrocephalus. Compared with the CCDC65 mice, the downregulation of CCDC65 in CCDC65 mice significantly increased the formation ability of lung cancer induced by urethane. In the subsequent investigation, we observed that CCDC65 functions as a tumor suppressor repressing cell proliferation and . Molecular mechanism showed that CCDC65 recruited E3 ubiquitin ligase FBXW7 to induce the ubiquitination degradation of c-Myc, an oncogenic transcription factor in tumors, and reduced c-Myc binding to ENO1 promoter, which suppressed the transcription of ENO1. In addition, CCDC65 also recruited FBXW7 to degrade ENO1 protein by ubiquitinated modulation. The downregulated ENO1 further reduced the phosphorylation activation of AKT1, which thus inactivated the cell cycle signal. Our data demonstrated that CCDC65 is a potential tumor suppressor by recruiting FBWX7 to suppress c-Myc/ENO1-induced cell cycle signal in lung adenocarcinoma.
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http://dx.doi.org/10.7150/ijbs.69332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274497PMC
July 2022

Outcome evaluation of decompression surgery combined with cerebellar tonsillectomy compared without cerebellar tonsillectomy for Chiari type I malformation patients.

Neurosurg Rev 2022 Jul 11. Epub 2022 Jul 11.

Department of Neurology, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi Province, China.

Background: Surgical intervention is an important treatment option to improve the prognosis for Chiari type I malformation (CM-I) patients. However, there is no consensus about surgical strategies. The article intends to evaluate the effect of decompression combined with or without cerebellar tonsillectomy in the treatment of CM-I.

Method: Following PRISMA's principles, Embase, PubMed, Web of Science, and Cochrane databases and references to relevant articles were searched to include only original articles on decompression combined with or without cerebellar tonsillectomy in CM-I patients. Through meta-analysis, the data on clinical improvement, radiological improvement, and complications were pooled.

Results: Nine articles, including 399 CM-I patients undergoing decompression alone and 343 undergoing decompression with cerebellar tonsillectomy, meet the inclusion standard. In comparison, the improvement rate of clinical symptoms or signs in patients with cerebellar tonsillectomy is higher and statistically significant (RR: 0.85, 95% CI: 0.75-0.95; P = 0.006). However, decompression with cerebellar tonsillectomy is also significantly higher in the incidence of postoperative complications (RR: 0.54, 95% CI: 0.36-0.80; P = 0.002). No statistical difference is discovered between the two groups in the improvement rate of syringomyelia (RR: 0.77, 95% CI: 0.57-1.03; P = 0.08).

Conclusions: Although decompression with cerebellar tonsillectomy is superior than decompression alone in clinical improvement for CM-I patients, it also faces a higher risk of postoperative complications. The reduction of syringomyelia in the two groups can be considered equally effective without significant differences. In the future, the results of the research require multicenter and large-scale randomized controlled trials to verify in clinical practice.

Trial Registration: CRD42022321750 (PROSPERO).
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http://dx.doi.org/10.1007/s10143-022-01829-wDOI Listing
July 2022

Targeted intelligent mesoporous polydopamine nanosystems for multimodal synergistic tumor treatment.

J Mater Chem B 2022 Jul 27;10(29):5644-5654. Epub 2022 Jul 27.

Chongqing Key Laboratory for Pharmaceutical Metabolism Research, Chongqing pharmacodynamic evaluation engineering technology research center, College of Pharmacy, Chongqing Medical University, Chongqing 400016, P. R. China.

Developing intelligent responsive platforms to carry out high-performance therapy is of great interest for the treatment of tumors and their metastases. However, effective drug loading, activity maintenance, off-target leakage, and response to collaborative therapy remain great challenges. Herein, a targeted intelligent responsive mesoporous polydopamine (MPDA) nanosystem was reported for use in gene-mediated photochemotherapy for synergistic tumor treatment. First, the MPDA was surface modified to maintain a positive charge near the surface and to impart active targeting. Then, gambogic acid (GA) was encapsulated in the MPDA, solidified by phase change materials (PCMs), and finally loaded with siRNA by electrostatic interactions to obtain the smart nanodelivery system ([email protected]/si). and experiments showed that it not only effectively avoids siRNA inactivation and accidental release of GA, but also possesses potential for targeted accumulation to tumor tissue and mild-temperature photothermal therapy and chemotherapy near infrared (NIR) radiation. Additionally, the release of siRNA could also effectively inhibit tumor invasion and metastasis to realize multimodal synergistic therapy. Overall, our studies provide a promising idea for synergistic tumor and metastasis treatment based on vector construction.
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http://dx.doi.org/10.1039/d2tb00973kDOI Listing
July 2022

Targeting p65 to inhibit Cas3 transcription by Onjisaponin B for radiation damage therapy in p65+/- mice.

Phytomedicine 2022 Sep 4;104:154317. Epub 2022 Jul 4.

School of food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address:

Background: In response to radiation injury, p65 becomes activated. The formation of p65 is one target of Onjisaponin B (OB), but it has not been studied in radioprotection. In addition, there is a binding site for p65 in the promoter region of Cas3. This study evaluates the use of OB as an intervention to modulate p65/Cas3 following radiation exposure.

Purpose: This study aimed to confirm that OB regulated the transcription of Cas3 via p65 to overcome radiation-induced damage.

Study Design And Methods: Cells and mice were exposed to X-rays at a dose of 6 Gy. Immunofluorescence was used to locate intracellular p65. For the protein and mRNA analyses, Western blotting and RT-qPCR-based assays were conducted accordingly. HE staining was used to observe pathological changes in tissues. DNA damage was detected by the comet assay and DNA ladder assay. Next, apoptosis was detected by flow cytometry and Hoechst staining.

Results: Compared with the radiation group, the expression levels of p-p65 and c-Cas3 in the drug group were significantly down-regulated by OB 20 µg/ml. When the expression of p65 was suppressed in V79 and TC cells, OB did not significantly inhibit the activation of p65 or Cas3 in response to irradiation, nor did it significantly inhibit the phosphorylation of p65 and subsequent nuclear translocation. Overexpression of p65 in V79 and MTEC-1 cells resulted in OB significantly inhibiting the activation of p65 and Cas3, and the phosphorylation and translocation of p65 into the nucleus. At 3 d for V79 cells and 24 h for MTEC-1 cells after radiation, compared with the Cas3 over plasmid transfection group, the drug transfection group had no significant effect on reducing apoptosis. In p65+/- mice, expression of the p65 gene was knocked down, leading to increased tissue apoptosis and inflammation, and serious tissue pathological changes. The inhibition of p65 activation by OB after radiation exposure was not apparent in the thymus, although it was observed in the lung.

Conclusions: OB interfered with radiation injury by targeting and regulating p65/Cas3. Therefore, it has been concluded that p65 is an important target molecule for the treatment of radiation injury.
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http://dx.doi.org/10.1016/j.phymed.2022.154317DOI Listing
September 2022

Sodium butyrate attenuates bovine mammary epithelial cell injury by inhibiting the formation of neutrophil extracellular traps.

Int Immunopharmacol 2022 Jul 8;110:109009. Epub 2022 Jul 8.

College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address:

Neutrophil extracellular traps (NETs) are an important means by which the body fights against exogenous bacteria. However, studies have shown that excessive NETs release can damage other cells. Accumulating evidence has shown that butyric acid can alleviate the inflammatory response of cells. However, the effect of butyric acid on Staphylococcus aureus-induced NETs formation and its underlying mechanism are still unclear. In this study, western blotting, immunofluorescence and CCK-8 assays were used to examine the effect of NETs formation by sodium butyrate (NaB). The results showed that NaB suppressed the release of S. aureus-induced NETs formation, as indicated by decreases in the levels of DNA, histones, myeloperoxidase, and neutrophil elastase. S. aureus can induce autophagy, and autophagy plays a key role in the formation of NETs. Our data showed that NaB activated mammalian target of rapamycin (mTOR) and the kinases protein kinase B (AKT) and unc-51 like kinase 1 (ULK1) at Ser757 and inhibited AMP-activated protein kinase (AMPK). To explore whether NaB inhibited the formation of NETs by inhibiting autophagy, we added 3-methyladenine (autophagy inhibitor) (3-MA, 5 mM) to bovine neutrophils, and the results showed that 3-MA significantly inhibited NETs release. Furthermore, we found that NETs and their component histones exhibited significantly increased the cytotoxic effects on bovine mammary epithelial cells (BMECs), indicating that NETs and their component histones play a key role in BMEC damage. In conclusion, NaB can reduce the excessive formation of NETs by inhibiting autophagy, thus reducing the damaging effect of NETs on BMECs.
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http://dx.doi.org/10.1016/j.intimp.2022.109009DOI Listing
July 2022

Relationship Between Negative Emotions and Job Burnout in Medical Staff During the Prevention and Control of the COVID-19 Epidemic: The Mediating Role of Psychological Resilience.

Front Psychiatry 2022 22;13:857134. Epub 2022 Jun 22.

Emergency Department, Zigong Fourth People's Hospital, Zigong, China.

We herein investigated the relationship between psychological status and the various emotions of medical staff during the prevention and control of coronavirus disease 2019 (COVID-19) epidemic. In this study, the convenience sampling method was used to select medical staff members as participants, and a cross-sectional study design was implemented. The instruments included the Burnout Clinical Subtype Questionnaire (BCSQ-36), the 10-item Connor-Davidson Resilience Scale (CD-RISC-10), the self-rated 16-item Quick Inventory of Depressive Symptomatology (QIDS-SR16), and the Self-rating Anxiety Scale (SAS). In total, 876 medical staff members were selected in this study. The CD-RISC-10 was negatively correlated with all other scales ( < 0.01). The hierarchical regression coefficients of the SAS and QIDS-SR16 against the BCSQ-36 mediated by the CD-RISC-10 were < 0.01, and the significance of the F values in all hierarchical regression equations was P < 0.01 (Sobel test, < 0.01). Medical staff burnout during the COVID-19 epidemic was affected by anxiety and depression, and psychological resilience had a mediating role. Attending to changes in the negative emotions of medical staff and improving their psychological resilience are beneficial to alleviate job burnout.
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http://dx.doi.org/10.3389/fpsyt.2022.857134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256987PMC
June 2022

A rare case of recurrent primary dumbbell-shaped spinal hydatidosis.

Radiol Case Rep 2022 Sep 1;17(9):3224-3227. Epub 2022 Jul 1.

Lanzhou University Second Hospital, Lanzhou, Gansu 730000, China.

Spinal hydatidosis, which affects the thoracic vertebrae, is not only an extremely rare occurrence, but is also characterized by a high recurrence rate. Here, we reported a case of 67-years-old man who presented with recurrent spinal hydatid disease. The condition was originally misdiagnosed as Schwannoma via medical imaging, but eventually confirmed by postoperative pathology. He was subjected to surgery, combined with adjuvant drug therapy. Unfortunately, he experienced recurrent spinal hydatid disease and had to undergo hydatid cyst excision in over 5 years.
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http://dx.doi.org/10.1016/j.radcr.2022.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256548PMC
September 2022

Nobiletin protects retinal ganglion cells in models of ocular hypertension in vivo and hypoxia in vitro.

Lab Invest 2022 Jul 8. Epub 2022 Jul 8.

Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China.

Glaucoma, a common cause of blindness, is characterized by the progressive loss of retinal ganglion cells (RGCs). Growing evidence suggests that nobiletin (NOB) is a promising neuroprotective drug; however, its effects on glaucomatous neurodegeneration remain unknown. Using rat models of microbead occlusion in vivo and primary RGCs model of hypoxia in vitro, we first demonstrate that NOB reduces RGC apoptosis by a TUNEL assay, Hoechst 33342 staining and FluoroGold (FG) retrograde labeling. This effect does not depend on intraocular pressure (IOP) lowering. Additionally, NOB partially restored the functional and structural damage of inner retinas, attenuated Müller glial activation and oxidative stress caused by ocular hypertension. At 2 weeks after IOP elevation, NOB further enhanced Nrf2/HO-1 pathway in RGCs to withstand the cumulative damage of ocular hypertension. With the administration of HO-1 inhibitor tin-protoporphyrin IX (SnPP), the protective effect of NOB was attenuated. Overall, these results indicate that NOB exerts an outstanding neuroprotective effect on RGCs of glaucomatous neurodegeneration. Besides, interventions to enhance activation of Nrf2/HO-1 pathway can slow the loss of RGCs and are viable therapies for glaucoma.
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http://dx.doi.org/10.1038/s41374-022-00813-8DOI Listing
July 2022

A scoping review on monitoring mental health using smart wearable devices.

Math Biosci Eng 2022 05;19(8):7899-7919

Department of Nursing, The Third Xiangya Hospital, Central South University, Changsha 410013, China.

With the continuous development of the times, social competition is becoming increasingly fierce, people are facing enormous pressure and mental health problems have become common. Long-term and persistent mental health problems can lead to severe mental disorders and even death in individuals. The real-time and accurate prediction of individual mental health has become an effective method to prevent the occurrence of mental health disorders. In recent years, smart wearable devices have been widely used for monitoring mental health and have played an important role. This paper provides a comprehensive review of the application fields, application mechanisms, common signals, common techniques and results of smart wearable devices for the detection of mental health problems, aiming to achieve more efficient and accurate prediction for individual mental health, and to achieve early identification, early prevention and early intervention to provide a reference for improving the level of individual mental health.
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http://dx.doi.org/10.3934/mbe.2022369DOI Listing
May 2022

Chemically labeled ThUBD permits rapid and super-sensitive imaging of polyubiquitination signals.

Analyst 2022 Jul 22;147(15):3434-3443. Epub 2022 Jul 22.

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Institute of Lifeomics, Beijing 102206, P. R. China.

Polyubiquitination signal deliver diverse cellular signal, which have been recognized as a sophisticated ubiquitin code. The perception and transduction of ubiquitination signal depend on the specificity and sensitivity of the ubiquitin-binding domain. Accurate and sensitive detection of polyubiquitination signal is crucial for revealing the dynamic cellular ubiquitin-regulated events. Western blotting (WB) and immunohistochemistry (IHC) are the most widely used biochemical strategies to detect ubiquitination signal on substrates under diverse physiological and pathological conditions. However, anti-ubiquitin antibodies fail to reflect polyubiquitination signal unbiasedly because of their strong preference for K63-linked ubiquitin chains. Herein, we demonstrated that our previously developed tandem hybrid ubiquitin-binding domain (ThUBD) chemically labeled with a reporter group such as horseradish peroxidase (ThUBD-HRP) could significantly improve the robustness and sensitivity of polyubiquitination signal detection. This advanced method was named TUF-WB Plus (TUF-WB). The TUF-WB method significantly increases the sensitivity and accuracy of ubiquitin detection and requires a shorter experimental operation time. Furthermore, it enables the ThUBD-HRP probe to function as a powerful tool for spatial polyubiquitination detection in cells by immunohistochemistry. Our newly developed ThUBD-HRP probe and TUF-WB method provide a robust and powerful tool for ubiquitination signal detection with hypersensitivity in an unbiased manner.
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http://dx.doi.org/10.1039/d2an00577hDOI Listing
July 2022
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