Publications by authors named "Ping Sun"

816 Publications

Expression of SAMHD1 and its mutation on prognosis of colon cancer.

Oncol Lett 2022 Sep 8;24(3):303. Epub 2022 Jul 8.

Department of Pathology, Wuxi No. 2 People's Hospital, Affiliated Wuxi Clinical College of Nantong University, Wuxi, Jiangsu 214002, P.R. China.

The expression of sterile α motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1) and its mutation play a key role in the prognosis of colon cancer. The aim of the present study was to investigate the mechanism and the role of SAMHD1 in colon cancer. Microarray data from 187 patients with colon cancer and 45 adjacent normal tissue obtained from the Gene Expression Omnibus (GEO) were analyzed. A protein-protein interaction (PPI) network was constructed to identify key genes associated with colon cancer prognosis. Cox proportional hazard regression and survival analyses were performed to identify the potential for SAMHD1 to serve as a prognostic biomarker. Immunohistochemistry (IHC) and immunofluorescence (IF) were performed to assess the expression levels and distribution of SAMHD1 in tissues and cells. Western blotting (WB) and Cell Counting Kit-8 (CCK-8) assays were used to identify the proliferation and apoptotic effects of SAMHD1 on HT-29 (Cas9-SAMHD1) cell lines. A total of 6,905 consistently differentially expressed genes were identified in the GEO database. Through the PPI network, SAMHD1 was found to be associated with Kirsten rat sarcoma virus (KRAS). SAMHD1 expression was negatively associated with KRAS. Proportional hazards regression and survival analyses demonstrated that low expression of SAMHD1 was associated with increased patient mortality. IHC and IF results demonstrated that SAMHD1 expression in patients with colon cancer was decreased compared with controls (both P<0.05). CCK-8 and WB results showed that proliferation was significantly promoted, and the expression levels of apoptosis-related proteins were significantly inhibited in the D137N and D311A groups as a result of a mutation in the deoxynucleoside triphosphohydrolase (dNTPase) site (both P<0.05 vs. wild-type). Proliferation was inhibited and apoptosis-related protein expression levels were promoted in the wild-type (WT) and D137N groups following 20 µg/ml 5-fluorouracil (5-FU) treatment (both P<0.05). WB and CCK-8 results showed cell proliferation was promoted and cell apoptosis-related protein expression was inhibited in the D137N group following treatment with 20 µg/ml 5-FU (all P<0.05) compared with the WT group. In conclusion, SAMHD1 expression was low in colon cancer. The dNTPase function of SAMHD1 may inhibit colon cancer cell proliferation and may enhance apoptosis. In addition, first-line chemotherapy with 5-FU has a time-dependent effect, which may provide novel options for clinical treatment of colon cancer.
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http://dx.doi.org/10.3892/ol.2022.13423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353240PMC
September 2022

Effects of polystyrene microplastics acute exposure in the liver of swordtail fish (Xiphophorus helleri) revealed by LC-MS metabolomics.

Sci Total Environ 2022 Aug 4:157772. Epub 2022 Aug 4.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471003, China. Electronic address:

As global pollution, microplastics pollution has aroused growing concerns. In our experiment, the effect of microplastics acute exposure on the liver of swordtail fish was investigated by using LC-MS metabolomics. Fishes treated with high concentration polystyrene microspheres (1 μm) for 72 h were divided into three concentration groups: (A) no microplastics, (B): 1 × 10 microspheres L, (C): 1 × 10 microspheres L. Metabolomic analysis indicated that exposure to microplastics caused alterations of metabolic profiles in swordtail fish, including 37 differential metabolites were identified in B vs. A, screened out ten significant metabolites, which involved 14 metabolic pathways. One hundred three differential metabolites were identified in C vs. A, screened out 16 significant metabolites, which involved 30 metabolic pathways. Six significant metabolites were overlapping in group B vs. A and C vs. A; they are 3-hydroxyanthranilic acid, l-histidine, citrulline, linoleic acid, pantothenate, and xanthine. In addition, four metabolic pathways are overlapping in group B vs. A and C vs. A; they are beta-alanine metabolism, biosynthesis of amino acids, linoleic acid metabolism, and aminoacyl-tRNA biosynthesis. These differential metabolites were involved in oxidative stress, immune function, energy metabolism, sugar metabolism, lipid metabolism, molecule transport, and weakened feed utilization, growth performance, nutrient metabolism, and animal growth. Furthermore, we found that the number of interfered amino acids and microplastics showed a dose-effect. In summary, great attention should be paid to the potential impact of microplastics on aquatic organisms.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157772DOI Listing
August 2022

The influence of alkali-modified biochar on the removal and release of Zn in bioretention systems: Adsorption and immobilization mechanism.

Environ Pollut 2022 Aug 2:119874. Epub 2022 Aug 2.

College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education of China, Ocean University of China, Qingdao, 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao, 266100, China.

Generally, Zn in stormwater runoff is considered as low toxicity, but in the senarios of roads and zinc-based materials roof runoff, the concentration of Zn becomes extremely high and cannot be ignored. Bioretention systems are used to remove heavy metals from stormwater runoff, while Zn adsorption is insufficient by conventional filler and is prone to secondary release when exposed to acid rain or high salinity runoff. This study integrated batch experiments and density functional theory calculation to investigate the mechanisms of how KOH-modified biochar (KBC) influences the removal and release of Zn in bioretention systems. The results revealed that KBC adsorbed 89.0-97.5% Zn in the influent, the main adsorption mechanism were complexation and precipitation, and precipitation is more important. In addition, 67% of Zn was immoblized as the residual form by KBC. In acidic and saline runoff, KBC reduced Zn secondary release by 43.6% and 37.08% compared to the results in the absence of KBC, which was attributed to the convertion of most dissolved Zn in acidic and saline runoff into residual Zn. Therefore, KBC has a considerable application potential not only to decontaminate the runoff of roads and Zn-containing roofs, but also to deal with secondary Zn release in acid rain or under the treatment of snow-melting agents.
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http://dx.doi.org/10.1016/j.envpol.2022.119874DOI Listing
August 2022

Simultaneous incorporation of gallium oxide and tantalum microparticles into micro-arc oxidation coating of titanium possessing antibacterial effect and stimulating cellular response.

Biomater Adv 2022 Apr 22;135:212736. Epub 2022 Apr 22.

Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. Electronic address:

Orthopedic implants with both osteogenesis and antibacterial functions are particularly promising for bone repair and substitutes. In this study, a micro-arc oxidation (MAO) coating containing titanium dioxide (TiO), gallium oxide (GaO) and tantalum oxide (TaO) on the titanium surface (MGT) was fabricated by dispersing GaO and Ta microparticles in the electrolyte. The results showed that the simultaneous incorporation of GaO and Ta microparticles into the MAO coating resulted in optimized surface performance (e.g., micro-topography, roughness, wettability, surface energy, and protein absorption) of MGT compared with pure titanium (pTi). In addition, MGT exhibited outstanding corrosion resistance owing to the presence of both GaO and Ta microparticles, which exhibit excellent corrosion resistance and their microparticles were incorporated into the micropores of the coating. Moreover, MGT with good cytocompatibility and optimized surface resulted in improved cellular responses (e.g., proliferation and osteogenic differentiation) of rat bone mesenchymal stem cells, which was attributed to Ta microparticles with outstanding osteogenic bioactivity. Furthermore, the excellent antibacterial effect of MGT was attributed to the slow release of Ga from the coating. Thus, the simultaneous incorporation of GaO and Ta microparticles into the MAO coating of MGT exhibited excellent cytocompatibility, osteogenic bioactivity, antibacterial functions, and corrosion resistance, suggesting that MGT possesses great potential for bone repair applications.
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http://dx.doi.org/10.1016/j.bioadv.2022.212736DOI Listing
April 2022

Community Structure of Phyllosphere Bacteria in Different Cultivars of Fingered Citron ( 'Fingered') and Their Correlations With Fragrance.

Front Plant Sci 2022 15;13:936252. Epub 2022 Jul 15.

Jinhua Academy of Agricultural Sciences, Jinhua, China.

In recent years, plant metabolomics and microbiome studies have suggested that the synthesis and secretion of plant secondary metabolites are affected by microbial-host symbiotic interactions. In this study, six varieties of fingered citron ( 'Fingered') are sampled to study their phyllosphere bacterial communities and volatile organic compounds (VOCs). High-throughput sequencing is used to sequence the V5-V7 region of the 16S rRNA of the fingered citron phyllosphere bacteria, and the results showed that Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes were the dominant bacterial phylum in the phyllosphere of fingered citron. There were significant differences in the phyllosphere bacteria community between XiuZhen and the remaining five varieties. The relative abundance of was highest in XiuZhen, and , , , and were also dominant. Among the remaining varieties, was the genus with the highest relative abundance, while the relative abundances of all the other genera were low. Headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to analyze and identify the aroma compounds of six different fingered citron, and a total of 76 aroma compounds were detected in six varieties. Pinene, geraniol, and linalool were found to be the primary VOCs that affect the aroma of fingered citron based on relative odor activity value. The correlation analysis showed 55 positive and 60 negative correlations between the phyllosphere bacterial flora and aroma compounds of fingered citron. The top 10 genera in the relative abundance were all significantly associated with aroma compounds. This study provides deep insight into the relation between bacteria and VOCs of fingered citron, and this may better explain the complexity of the analysis of bacterial and metabolic interactions.
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http://dx.doi.org/10.3389/fpls.2022.936252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335054PMC
July 2022

Preparation of resistant glucan by high pressure processing (HPP) and enzymatic methods increases indigestibility.

Int J Biol Macromol 2022 Jul 26;219:150-158. Epub 2022 Jul 26.

Qilu University of Technology (Shandong Academy of Sciences), Shandong Food Ferment Industry Research & Design Institute, Jinan 250000, China. Electronic address:

This study describes a new method for producing high-quality resistant glucan by characterizing the structural mechanism of indigestibility. The structures and properties of resistant glucans were characterized before and after in vitro simulated digestion. The results demonstrated that high-pressure processing (HPP) led to the complete disappearance of crystal peaks and increased the efficiency of the two enzymes (α-amylase and transglucosidase). Moreover, α-1,6 and β-linkages were abundant in the connecting parts of the long and branching chains in the resulting resistant glucans, thus hindering the ability of digestive enzymes to hydrolyze short chains with a degree of polymerization (DP) ≤ 6. In addition, transglucosidase activity led to a higher proportion of short chains (DP 3-6), further promoting indigestibility. We demonstrated that, without rectification and decolorization, the dietary fiber content was >75 %, and the degree of branching increased to 50.9 %, indicating higher indigestibility than that resistant glucans produced by traditional methods.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.173DOI Listing
July 2022

Physical Activities and Associated Factors Among HIV/AIDS Patients: A Questionnaire Survey.

Patient Prefer Adherence 2022 19;16:1703-1712. Epub 2022 Jul 19.

Infectious Disease, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People's Republic of China.

Purpose: Regular physical activity is essential for maintaining health and improving people's overall quality of life living with HIV; improving metabolic side effects associated with taking highly active antiretroviral therapy (HAART). This study investigated physical activity levels and associated factors among HIV/AIDS patients.

Patients And Methods: A single-center, cross-sectional study was conducted from July to September 2021. A convenience sample of 377 HIV/AIDS patients was recruited from a medical center in Zhuhai, China. Data were collected through self-report questionnaires and patients' medical records.

Results: The average physical activity of HIV/AIDS patients was 1007.15±868.63 met-min/w; 53.8% of patients expressed medium-level activity, and 46.2% in low-level activity. Log-link gamma generalized linear model results showed that gender, T-CD4 at the latest visit, self-efficacy, and perceived barriers were significant predictors of assessing physical activity levels.

Conclusion: Nearly half of HIV/AIDS patients have low levels of physical activity. In particular, women and patients with lower CD4 cells, lower self-efficacy, and more perceived barriers had lower levels of physical activity. More knowledge is needed on the causes of physical inactivity among people with HIV, and physical activity programs need to be developed for people living with HIV in developing countries.
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http://dx.doi.org/10.2147/PPA.S360517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309313PMC
July 2022

Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I.

Drug Deliv Transl Res 2022 Jul 25. Epub 2022 Jul 25.

Grade Three Laboratory of Traditional Chinese Medicine Preparation of the National Administration of Traditional Chinese Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250014, China.

Polyphyllin I (PPI), an effective active ingredient in Paris polyphylla, has a diverse set of pharmacological properties. However, due to its poor solubility and oral absorption, its application and development are limited. In the study, we were committed to improving the solubility of PPI by developing a self-microemulsifying drug delivery system of PPI (PPI-SMEDDS), screening the best preparation process, and evaluating the quality and the in vivo pharmacokinetics of PPI, and PPI-SMEDDS following oral administration to rats were also studied. In addition, the pharmacological activities against human lung adenocarcinoma cell A549 in vitro were assessed. The best formulation had 15.89% ethyl oleate, 47.38% Cremophor RH40, and 36.73% 1,2 propylene glycol. The produced PPI-SMEDDS was clear and transparent, with an average particle size of 24.51 nm and a zeta potential of -17.54 ± 0.51 mV. In vitro, the cumulative release rate of PPI-SMEDDS was nearly 80% within 2 h. PPI-SMEDDS had a substantially greater area under the curve than PPI following oral treatment in rats, and the relative bioavailability of PPI in rats was 278.99%. More importantly, the anti-tumor effect of PPI-SMEDDS in vitro was significantly greater than that of PPI. These findings suggested that PPI-SMEDDS has the potential to improve the solubility, oral bioavailability of PPI, and anti-tumor effect, laying the groundwork for future research on the new PPI dosage form.
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http://dx.doi.org/10.1007/s13346-022-01212-8DOI Listing
July 2022

Ciclopirox inhibits NLRP3 inflammasome activation via protecting mitochondria and ameliorates imiquimod-induced psoriatic inflammation in mice.

Eur J Pharmacol 2022 Jul 19;930:175156. Epub 2022 Jul 19.

Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, China. Electronic address:

The maturation and secretion of interleukin-1β (IL-1β) mediated by NLRP3 inflammasome activation plays an important role in the progression of many inflammatory diseases. Inhibition of NLRP3 inflammasome activation may be a promising strategy to treat these inflammation-driven diseases, such as psoriasis. As a broad-spectrum antifungal agent, ciclopirox (CPX) is widely used in the treatment of dermatomycosis. Although CPX has been reported to have anti-inflammatory effects in many studies, there has been little research into its underlying mechanisms. In our study, CPX reduced lipopolysaccharide (LPS)/nigericin-induced NLRP3 inflammasome activation (IC: 1.684 μM). Mechanistically, CPX upregulated peroxisome proliferator-activated receptor-γ coactivator-1α expression (by 82.7% at 5 μM and 87.5% at 10 μM) to protect mitochondria. Our studies showed that CPX reduced mitochondrial reactive oxygen species production, increased mitochondrial membrane potential, elevated mitochondrial biosynthesis, and up-regulated intracellular adenosine triphosphate level. Furthermore, treatment with CPX promoted the up-regulation of mRNA expression, which involved mitochondrial biosynthesis (NRF1, NRF2, TFAM) and antioxidation (SOD1 and CAT). In addition, CPX ameliorated inflammatory response in imiquimod-induced psoriasis mice. This study provides a potential pharmacological mechanism for CPX to treat psoriasis and other NLRP3-driven inflammatory diseases.
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http://dx.doi.org/10.1016/j.ejphar.2022.175156DOI Listing
July 2022

Varying Clinical Phenotypes of Mitochondrial DNA T12811C Mutation: A Case Series Report.

Front Med (Lausanne) 2022 4;9:912103. Epub 2022 Jul 4.

Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.

The T12811C mitochondrial DNA (mtDNA) mutation has been reported in Leber hereditary optic neuropathy (LHON) previously, with vision loss as the main manifestation. The involvement of other organ systems, including the central and peripheral nervous system, heart, and extraocular muscles, has not been well described. This case series report investigated four patients with T12811C mtDNA mutation, verified through a next generation sequencing. Two male patients presented with bilateral subacute visual decrease combined with involvement of multiple organ systems: leukoencephalopathy, hypertrophic cardiomyopathy, neurosensory deafness, spinal cord lesion and peripheral neuropathies. Two female patients presented with progressive ptosis and ophthalmoplegia, one of whom also manifested optic atrophy. This study found out that patients harboring T12811C mtDNA mutation manifested not only as vision loss, but also as a multi-system disorder affecting the nervous system, heart, and extraocular muscles.
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http://dx.doi.org/10.3389/fmed.2022.912103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291510PMC
July 2022

Change of Cytokines in Chronic Hepatitis B Patients and HBeAg are Positively Correlated with HBV RNA, Based on Real-world Study.

J Clin Transl Hepatol 2022 Jun 18;10(3):390-397. Epub 2021 Sep 18.

Outpatient Department of Hepatitis, The Sixth Affiliated People's Hospital of Dalian Medical University, Dalian, Liaoning, China.

Background And Aims: The natural course of chronic hepatitis B virus (HBV) infection is widely studied; however, follow-up studies of the same patients are scanty. Here, we studied the dynamic changes of serum HBV RNA and cytokines in hepatitis B virus e antigen (HBeAg)-positive patients treated with entecavir (ETV) to explore the relationship between the HBV serum viral nucleic acids and host immunity.

Methods: Thirty-three chronic hepatitis B patients who are HBeAg-positive, with high virus load (HBV DNA >20,000 IU/mL), and received standard nucleos(t)ide analogue (NA) antiviral therapy (ETV) for more than 48 weeks were included. The serum levels of HBV nucleic acids and selected cytokines were measured at 0, 12, 24, and 48 weeks respectively.

Results: Serum HBV RNA could still be detected while serum HBV DNA had fallen below the detection limit in patients treated with ETV. There was a strong positive correlation between HBV RNA and HBeAg, with a concomitant decrease in the secretion of cytokines from type 1 helper T (Th1)/type 2 helper T (Th2)/interleukin (IL)-17 producing T (Th17) cells. IL-4 and IL-10 were the main cytokines negatively associated with serum HBV RNA.

Conclusions: HBeAg can be used to reflect the load of HBV RNA indirectly, because serum HBV RNA has not been widely used in clinical practice. Meanwhile, serum IL-4 and IL-10 might be explored in combination with HBV RNA in guiding future clinical antiviral therapy.
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http://dx.doi.org/10.14218/JCTH.2021.00160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240249PMC
June 2022

Pregnancy and Adverse Obstetric Outcomes After Hysteroscopic Resection: A Systematic Review and Meta-Analysis.

Front Surg 2022 27;9:889696. Epub 2022 Jun 27.

School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objective: Although the randomized controlled trial (RCT) of the efficacy of hysteroscopic resection in women with uterine septum has not shown any significant correlation in recent research, motivation for deeper study remains insufficient. In this study, the objective was to determine pregnancy-related outcomes, along with adverse obstetric outcomes, following hysteroscopic resection and also to determine whether women with hysteroscopic resection bear the same outcomes as women with normal uterine cavities.

Search Methods: From January 1995 to February 2022, a systematic literature review was conducted to identify all studies published concerning the gestation outcomes of women with and without hysteroscopic resection while comparing the gestation outcomes of women after hysteroscopic resection and with a normal uterine cavity. Our primary outcome was the live birth rate (LBR). The secondary outcomes were term delivery, preterm delivery, spontaneous miscarriage, malpresentation, cesarean section, and other adverse obstetric outcomes.

Results: 22 studies were included in this meta-analysis. The control groups of 14 studies were treated women, and the control groups of the other 8 studies were patients bearing a normal uterine cavity. Hysteroscopic resection was related to a higher rate of term delivery (OR = 2.26, 95% CI, 1.26-4.05), and a lower rate of spontaneous abortion (OR = 0.50, 95% CI, 0.27-0.93), and a lower rate of malpresentation (OR = 0.31, 95% CI, 0.19-0.50). Nevertheless, in comparison with the normal uterus group, the rates of preterm birth, cesarean section, and postpartum hemorrhage after resection did not return to normal levels.

Conclusion: Hysteroscopic resection can effectively reduce the risk of abortion and malpresentation in patients possessing a uterine septum while increasing the term delivery rate. Although well-designed RCTs should confirm our meta-analysis, it still bears recommending to patients.
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http://dx.doi.org/10.3389/fsurg.2022.889696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271824PMC
June 2022

The therapeutic effect of Fufang Zhenshu Tiaozhi (FTZ) on osteoclastogenesis and ovariectomized-induced bone loss: evidence from network pharmacology, molecular docking and experimental validation.

Aging (Albany NY) 2022 Jul 12;14(14):5727-5748. Epub 2022 Jul 12.

Department of Endocrinology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510000, Guangdong, China.

Fufang Zhenshu Tiaozhi (FTZ) has been widely used in clinical practice and proven to be effective against aging-induced osteoporosis in mice. This study aimed to explore the mechanism of FTZ against osteoclastogenesis and ovariectomized-induced (OVX) bone loss through the network pharmacology approach. The ingredients of FTZ were collected from the previous UPLC results, and their putative targets were obtained through multiple databases. Differentially expressed genes (DEGs) during osteoclastogenesis were identified through multi-microarrays analysis. The common genes between FTZ targets and DEGs were used to perform enrichment analyses through the clusterProfier package. The affinity between all FTZ compounds and enriched genes was validated by molecular docking. The effects of FTZ on osteoclastogenesis and bone resorption were evaluated by TRAP staining, bone resorption assay and RT-qPCR , while its effects on bone loss by ELISA and Micro-CT . Enrichment analyses indicated that the inhibitory effects of FTZ may primarily involve the regulation of inflammation, osteoclastogenesis, as well as TNF-α signaling pathway. 130 pairs docking results confirmed FTZ ingredients have good binding activities with TNF-α pathway enriched genes. FTZ treatment significantly reduced TRAP, TNF-α, IL-6 serum levels and increased bone volume in OVX mice. Consistently, experiments revealed that FTZ-containing serum significantly inhibited osteoclast differentiation, bone resorption, and osteoclast related mRNA expression. This study revealed the candidate targets of FTZ and its potential mechanism in inhibiting osteoclastogenesis and bone loss induced by OVX, which will pave the way for the application of FTZ in the postmenopausal osteoporosis treatment.
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http://dx.doi.org/10.18632/aging.204172DOI Listing
July 2022

Berberine inhibited the formation of metastasis by intervening the secondary homing of colorectal cancer cells in the blood circulation to the lung and liver through HEY2.

Phytomedicine 2022 Sep 27;104:154303. Epub 2022 Jun 27.

Department of Emergency, The Affiliated Wuxi NO. 2 People's Hospital of Nanjing Medical University, No. 68 Zhongshan Road, Wuxi 214000, PR China. Electronic address:

Background: Metastasis is the leading cause of death in patients with colorectal cancer (CRC). The 5-year survival rate of CRC patients in whom the cancer has spread to distant sites is 13.5%. The most common sites of CRC metastasis are liver and lung. The principal therapies for CRC metastatic disease are surgery, but its benefits are limited.

Purpose: This study aimed to reveal the regulatory mechanism of berberine on secondary homing of CRC cells to form metastatic focus. This was more valuable than the previous direct study of the migration and metastasis characteristics of CRC cells.

Methods: In this study, we used the functional enrichment analysis of differentially expressed genes after berberine treatment and investigated co-expression modules related with CRC metastasis by WGCNA. PPI and survival analyses of significant modules were also conducted. The biological functions of berberine in CRC lung and liver metastasis were investigated by a series of in vitro and in vivo experiments: MTT, colony formation and mouse tail vein injection. And we scanned through the entire extracellular domain of HEY2 protein for autodocking analysis with berberine.

Results: We found the differentially expressed genes (DEGs) after berberine treatment were related with cancer progression and metastasis related pathways. Through WGCNA analysis, four cancer progression and metastasis related modules were detected. After PPI and survival analysis, we identified and validated HEY2 as a hub gene, high expression and poor survival at the metastatic stage. Functionally, berberine inhibited the survival, invasion and migration of CRC cells in vitro and in vivo. Mechanistically, berberine treatment down-regulated the expression of HEY2, metastasis related protein E-cadherin, β-catenin and Cyclin D1 during Mesenchymal epithelial transformation (MET). Berberine and HEY2 showed a significant interaction, and berberine binded to HEY2 protein at the residue HIS-99 interface with a hydrogen-bond distance of 1.9A.

Conclusions: We revealed that berberine could significantly inhibit the expression of hub gene HEY2 and metastasis related proteins E-cadherin and β-catenin and Cyclin D1 during MET in CRC lung and liver metastases. In total, HEY2 was a promising candidate biomarker for prognosis and molecular characteristics in CRC metastasis.
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http://dx.doi.org/10.1016/j.phymed.2022.154303DOI Listing
September 2022

Giant Cell Arteritis Presenting With Ocular Symptoms: Clinical Characteristics and Multimodal Imaging in a Chinese Case Series.

Front Med (Lausanne) 2022 20;9:885463. Epub 2022 Jun 20.

Department of Ophthalmology, Eye Ear Nose and Throat Hospital of Fudan University, Shanghai, China.

Purpose: To evaluate demographic and clinical characteristics of a Chinese population with giant cell arteritis using multimodal imaging focusing on ophthalmic examinations.

Design: Retrospective observational case series.

Materials And Methods: In the neuro-ophthalmology division of the Eye, Ear, Nose, and Throat Hospital, Shanghai, we evaluated the demographic and clinical characteristics of patients diagnosed with giant cell arteritis between January 2016 and June 2021. Results of routine ophthalmic examinations including fundus examination, optical coherence tomography, color duplex ultrasonography of ocular and superficial temporal arteries, orbital magnetic resonance imaging, and superficial temporal artery biopsy were evaluated.

Results: A total of 15 patients (22 eyes; ten male and five female) were evaluated with a mean age of 77.0 ± 8.5 years. Among them, seven had bilateral involvement that occurred simultaneously or sequentially. Twelve patients presented with arteritic anterior ischemic optic neuropathy, two with arteritic anterior ischemic optic neuropathy combined with cilioretinal artery occlusion, and one with cotton-wool spots. In acute stages of optic neuropathy and retinopathy, optical coherence tomography revealed optic disc edema, thickening of the inner retinal nerve fiber layer and ganglion cell layer, and loss of layer structure. In late stages, optical coherence tomography revealed diffuse atrophy of the inner retina. The "halo" sign was observed in 12 patients in the superficial temporal artery ultrasound, and seven out of eight patients who underwent biopsy demonstrated classic giant cell arteritis pathological changes. Most patients having poor visual acuity but ability to perceive light; 10/22 eyes had permanent vision loss.

Conclusion: Although rare in Asians, giant cell arteritis may be underdiagnosed among elderly Chinese patients presenting with anterior ischemic optic neuropathy. Non-invasive superficial temporal artery ultrasound detecting inflammatory thickening of the intima as the "halo" sign combined with routine elevated erythrocyte sedimentation rate and C-reactive protein may be helpful in diagnosing patients with a high probability of having giant cell arteritis.
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http://dx.doi.org/10.3389/fmed.2022.885463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251180PMC
June 2022

Partial response to Zercepac® plus chemotherapy in triple-positive advanced breast cancer with changed molecular typing.

Minerva Med 2022 Jul 5. Epub 2022 Jul 5.

The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong Province, P.R. China -

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http://dx.doi.org/10.23736/S0026-4806.22.08243-XDOI Listing
July 2022

ST8SIA6-AS1 Promotes the Epithelial-to-Mesenchymal Transition and Angiogenesis of Pituitary Adenoma.

J Oncol 2022 22;2022:7960261. Epub 2022 Jun 22.

Department of Traditional Chinese Medicine, People' Hospital of Chengyang, Qingdao, 266109 Shandong, China.

To investigate the effect of long noncoding RNA ST8SIA6-AS1 on the epithelial-to-mesenchymal transition (EMT) and angiogenesis of pituitary adenoma and its possible mechanism. The expression levels of ST8SIA6-AS1 and HOXA9 in noninvasive pituitary adenoma and invasive pituitary adenoma were detected using qRT-PCR. sh-ST8SIA6-AS1 transfection silenced the expression of ST8SIA6-AS1 in GH3 and GTI-1 cells. The effects of ST8SIA6-AS1 on the proliferation, invasion, angiogenesis, and EMT of GH3 and GTI-1 pituitary adenoma cells were detected. The migration ability of cells was detected through scratch assay. Dual luciferase analysis verified the targeting relationship between ST8SIA6-AS1 and miR-5195-3p. ST8SIA6-AS1 and HOXA9 were highly expressed in invasive pituitary adenoma. In pituitary adenomas, miR-5195-3p directly targeted HOXA9. miR-5195-3p is the target gene of ST8SIA6-AS1. ST8SIA6-AS1 knockdown inhibited the proliferation, invasion, angiogenesis, and EMT of pituitary adenoma. HOXA9 expression mediates the biological effect of ST8SIA6-AS1. ST8SIA6-AS1 targets miR-5195-3p to regulate the expression of HOXA9 and promote the EMT of pituitary adenomas.
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http://dx.doi.org/10.1155/2022/7960261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242794PMC
June 2022

Exploring the Core Genes of Schizophrenia Based on Bioinformatics Analysis.

Genes (Basel) 2022 May 27;13(6). Epub 2022 May 27.

Clinical Research Center and Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.

Schizophrenia is a clinical syndrome composed of a group of symptoms involving many obstacles such as perception, thinking, emotion, behavior, and the disharmony of mental activities. Schizophrenia is one of the top ten causes of disability globally, accounting for about 1% of the global population. Previous studies have shown that schizophrenia has solid genetic characteristics. However, the diagnosis of schizophrenia mainly depends on symptomatic manifestations, and no gene can be used as a clear diagnostic marker at present. This study explored the hub genes of schizophrenia by bioinformatics analysis. Three datasets were selected and downloaded from the GEO database (GSE53987, GSE21138, and GSE27383). GEO2R, NCBI's online analysis tool, is used to screen out significant gene expression differences. The genes were functionally enriched by GO and KEGG enrichment analysis. On this basis, the hub genes were explored through Cytoscape software, and the immune infiltration analysis and diagnostic value of the screened hub genes were judged. Finally, four hub genes (NFKBIA, CDKN1A, BTG2, GADD45B) were screened. There was a significant correlation between two hub genes (NFKBIA, BTG2) and resting memory CD4 T cells. The ROC curve results showed that all four hub genes had diagnostic value.
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http://dx.doi.org/10.3390/genes13060967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223283PMC
May 2022

High Expression of RECQL Protein in ER-Positive Breast Tumours Is Associated With a Better Survival.

Front Oncol 2022 31;12:877617. Epub 2022 May 31.

Women's College Research Institute, Women's College Hospital, University of Toronto, Toronto, ON, Canada.

Background: RECQL (also known as RECQ1 and RECQL1) is a gene of recent interest in breast cancer and an association between high levels of RECQL protein in breast cancer tumour cells and good survival of patients has been reported.

Methods: To validate this association, we measured the RECQL protein levels in tumours of 933 breast cancer patients using immunohistochemistry analysis and followed the patients for death from breast cancer.

Results: Women with a level of RECQL protein above the 75th percentile had better 15-year disease-specific survival among ER-positive patients (62.5% vs. 48.7%, HR= 0.72, 95%CI= 0.52-0.98, p-value = 0.04), but not among ER- patients (48.9% vs. 48.0%, HR= 1.07, 95%CI= 0.67-1.69, p-value= 0.79). Among the ER-negative patients, high RECQL protein levels were associated with better survival among women who received tamoxifen treatment (67.0% vs. 51.5%, HR= 0.64, 95%CI= 0.41-0.99, p-value= 0.04).

Conclusion: RECQL might be a new predictive marker for tamoxifen treatment among ER-positive patients.
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http://dx.doi.org/10.3389/fonc.2022.877617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195420PMC
May 2022

Impact of germline mutations in cancer-predisposing genes on long-term survival in patients with epithelial ovarian cancer.

Br J Cancer 2022 Jun 16. Epub 2022 Jun 16.

Women's College Research Institute, Women's College Hospital, 76 Grenville Street, Toronto, ON, Canada.

Background: Several clinical and tumour factors impact on ovarian cancer survival. It is important to evaluate if germline mutations impact long-term outcomes among patients with epithelial ovarian cancer.

Methods: We followed 1422 Ontario women with ovarian cancer. Clinical information was obtained from medical records and vital status was determined by registry linkage. Germline genetic testing was performed for 12 susceptibility genes. We estimated 20-year cancer-specific survival according to various factors.

Results: Twenty-year survival was inferior for women with serous cancers vs. other types (22.3% vs. 68.6%; P < 0.0001). Of the 1422 patients, 248 (17.4%) carried a germline mutation; 119 BRCA1; 75 BRCA2; 7 in a mismatch repair (MMR) gene and 47 in one of seven other genes. Among serous patients, 20-year survival was 28.9% for similar for women with a BRCA1 (28.9%), BRCA2 (21.2%) or no mutation (21.6%). Among endometrioid patients, 20-year survival was poor for women with a BRCA vs. no mutation (47.3% vs. 70.4%; P = 0.004). Six of the seven MMR mutation carriers are currently alive, while all three PALB2 mutation carriers died within 3 years of diagnosis. Among women with Stage III/IV serous cancers, 20-year survival was 9.4% for those with vs. 46.5% for those with no residual disease (HR = 2.91; 95% CI 2.12-4.09, P < 0.0001).

Conclusions: The most important predictor of long-term survival was no residual disease post surgery. BRCA mutation status was not predictive of long-term survival while those with MMR mutations had excellent survival. Larger studies on PALB2 carriers are needed.
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http://dx.doi.org/10.1038/s41416-022-01840-4DOI Listing
June 2022

Retrospective Analysis of Real-World Management of EGFR-Mutated Advanced NSCLC, After First-Line EGFR-TKI Treatment: US Treatment Patterns, Attrition, and Survival Data.

Drugs Real World Outcomes 2022 Jun 3. Epub 2022 Jun 3.

Real World Science and Digital, AstraZeneca, Cambridge, UK.

Background And Objectives: Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are standard-of-care first-line (1L) treatment for EGFR mutation-positive advanced/metastatic non-small cell lung cancer. In 2015, osimertinib, a third-generation EGFR-TKI, received US accelerated approval for second-line (2L) EGFR T790M-positive non-small cell lung cancer treatment. The objective of this US study was to characterize treatment patterns, attrition, and survival in EGFR mutation-positive non-small cell lung cancer, after 1L first-/second-generation EGFR-TKI treatment.

Methods: We retrospectively analyzed 1029 patients diagnosed with stage IIIB/IV non-small cell lung cancer from 1 January, 2011 to 31 December, 2018 using the US electronic medical record CancerLinQ Discovery database. Demographic/disease characteristics, EGFR mutations, treatments, and death dates were collected.

Results: From 1 January, 2011 to 31 December, 2014 (< 2015 cohort), 519 patients received 1L EGFR-TKIs and 510 between 1 January, 2015 and 31 December, 2018 (≥ 2015 cohort). Median follow-up from advanced diagnosis was 19.8 months (interquartile range: 9.9-33.4 months). Twenty-eight percent of patients (288/1029) died without receiving 2L, and 52% (539/1029) initiated 2L with 35% (186/539) receiving osimertinib; in the < 2015 and ≥ 2015 cohorts, the same proportion initiated 2L (52%; 272/519 vs 267/510, respectively). Median overall survival from advanced diagnosis for patients initially diagnosed with stage I-IIIA disease was 43.3 months (95% confidence interval 30.9-73.7), vs 26.4 months (95% confidence interval 24.4-28.1) for stage IIIB-IV; all-cause mortality hazard ratio: 1.56 (95% confidence interval 1.2-2.0; p = 0.001).

Conclusions: We identified disease stage, performance status, and central nervous system metastasis as survival predictors, highlighting the importance of optimal 1L treatment selection. Over a quarter of patients died before initiating 2L; half progressed after 1L and received 2L, of whom a third received 2L osimertinib.
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http://dx.doi.org/10.1007/s40801-022-00302-wDOI Listing
June 2022

Establishment of an induced pluripotent stem cell (iPSC) line (INNDSUi001-A) from a healthy female Chinese Han.

Stem Cell Res 2022 07 23;62:102819. Epub 2022 May 23.

Research Institute of Neuromuscular and Neurodegenerative Diseases and Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, People's Republic of China. Electronic address:

We have established the human induced pluripotent stem cell (hiPSC) line (INNDSUi001-A) from skin fibroblasts of a healthy 24-year-old female individual by using non-integrating reprogramming method. The resulted cells had typical features of embryonic stem cell as indicated by expression of specific pluripotency markers and had the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a healthy control for disease study.
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http://dx.doi.org/10.1016/j.scr.2022.102819DOI Listing
July 2022

Carbon Emissions of the Tourism Telecoupling System: Theoretical Framework, Model Specification and Synthesis Effects.

Int J Environ Res Public Health 2022 05 14;19(10). Epub 2022 May 14.

School of Geography and Ocean Science, Nanjing University, Nanjing 210023, China.

The flows of people and material attributed to international tourism exert a major impact on the global environment. Tourism carbon emissions is the main indicator in this context. However, previous studies focused on estimating the emissions of destinations, ignoring the embodied emissions in tourists' origins and other areas. This study provides a comprehensive framework of a tourism telecoupling system. Taking China's international tourism as an example, we estimate the carbon emissions of its tourism telecoupling system based on the Tourism Satellite Account and input-output model. We find that (1) the proposal of a tourism telecoupling system provides a new perspective for analyzing the carbon emissions of a tourism system. The sending system (origins) and indirect spillover system (resource suppliers) have been ignored in previous studies. (2) In the telecoupling system of China's international tourism, the emission reduction effect of the sending system is significant. (3) The direct spillover system (transit) and indirect spillover system's spatial transfer effects of environment responsibility are remarkable. (4) There is a large carbon trade implied in international tourism. This study makes us pay attention to the carbon emissions of tourists' origins and the implied carbon trading in tourism flows.
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http://dx.doi.org/10.3390/ijerph19105984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142052PMC
May 2022

The texture of plant protein-based meat analogs by high moisture extrusion: A review.

J Texture Stud 2022 May 17. Epub 2022 May 17.

College of Food Science, Northeast Agricultural University, Harbin, China.

Meat analogs produced by high moisture extrusion (HME) are considered to be one of the products that have great potential for replacing real meat. The key issue as a meat analog is whether the texture can meet the standards of real meat. Nowadays, there have been some advances in the textural characterization of meat analogs, which are discussed in detail in this review. Firstly, this review describes the current characterizations of meat analogs in terms of fiber structure, hardness, springiness, tensile resistant force and sensory evaluation. Then, methods for analyzing the texture of meat analogs, such as texture analyzer, microstructure-based methods, and other methods for characterizing fiber structure, are summarized. In addition, these characterizations are discussed in relation to the factors that influence the texture of meat analogs during HME. Finally, we propose priorities and some promising methods for future meat analogs conformation studies.
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http://dx.doi.org/10.1111/jtxs.12697DOI Listing
May 2022

Effect of a High Protein, Low Glycemic Index Dietary Intervention on Metabolic Dysfunction-Associated Fatty Liver Disease: A Randomized Controlled Trial.

Front Nutr 2022 27;9:863834. Epub 2022 Apr 27.

Department of Health Management and Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: Metabolic dysfunction-associated fatty liver disease (MAFLD) affects people at an increasingly younger age. The primary treatment for patients with MAFLD is diet-induced weight loss; however, excessive dieting is poorly effective.

Objectives: The aim of this trial was to evaluate whether a high protein and low glycemic index (HPLG) dietary intervention would result in improvement of controlled attenuation parameter (CAP) and related metabolic markers in MAFLD.

Methods: A 12-week controlled, parallel-group, randomized intervention trial was performed. A number of 63 participants with MAFLD were enrolled and randomized between the HPLG dietary group and the balanced diet control group. Both diets had the same hypocaloric level and were prescribed within food limit lists. The primary outcome was CAP. The main secondary outcomes were weight loss and improvement of metabolism-related indexes at week 12 after the program initiation.

Results: A total of 59 participants completed the intervention and were included in the final analysis. The mean age was 39.3 ± 8.9 years and 66.1% were men. In this trial, protein and carbohydrate intakes were significantly higher and lower, respectively, in the HPLG group compared to controls ( < 0.001). At week 12, CAP was significantly reduced in both groups ( < 0.001). However, a significantly greater reduction in liver fat was observed in the HPLG group compared to the control group ( = 0.011), with mean relative reductions of 30.90 dB/m (95% CI, 21.53 to 40.26, < 0.001) and 15.43 dB/m (95% CI, 7.57 to 23.30, < 0.001), respectively. From baseline to week 12, a significantly greater loss in bodyweight was recorded in participants in the HPLG group (6.52 kg; 95% CI, 5.50 to 7.54, < 0.001) compared to control subjects (2.00 kg; 95% CI, 0.89 to 3.11, = 0.001). Moreover, body fat percentage in the HPLG group was significantly reduced compared with the control group ( = 0.002). Within-group improvements in visceral fat, blood pressure, cardiovascular risk factors, and blood glucose-related indicators were detected in patients with MAFLD assigned to the HPLG diet ( < 0.05), but not in those prescribed the control diet ( > 0.05).

Conclusion: Under our experimental conditions, and compared to the traditional balanced diet, an HPLG diet led to a significant CAP remission, bodyweight or fat reduction, and improvement of metabolic markers in patients with MAFLD.

Clinical Trial Registration: ClinicalTrials.gov, identifier: NCT03972631.
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http://dx.doi.org/10.3389/fnut.2022.863834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097015PMC
April 2022

Quantitative evaluation of the synergistic effect of biochar and plants on immobilization of Pb.

J Environ Manage 2022 Aug 6;316:115200. Epub 2022 May 6.

College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education of China, Ocean University of China, Qingdao, 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao, 266100, China.

Biochar and plant cooperation in remediation of heavy metal contaminated soil is effective and important, but there still have knowledge gaps of synergistic effect between the two and the synergistic pathway has not been clarified. We prepared the Enteromorpha prolifera biochar at 400 °C and 600 °C (denoted as BC400 and BC600). The Pb fractions changes in soil and Pb toxicity in Brassica juncea were investigated by adding 30 g kg biochar to soil containing 1200 mg kg Pb in a pot experiment. There was a significant synergistic effect between biochar and plants on Pb immobilization in soil, according to the "E > 0" of Pb fractions in the interaction equation. Pb immobilization rates of biochar-plant treatments (BJBC4 and BJBC6) were 12.47% and 11.38% higher than biochar treatment (BC4, BC6), and 17.66% and 16.28% plant treatment (BJ). BJBC4 had a better immobilization effect than BJBC6. Biochar alleviated the phytotoxicity of Pb by increasing the antioxidant enzymes activities of plants. These results indicated two synergistic pathways: (1) The high pH and oxygen-containing functional groups of biochar could immobilize Pb through ion exchange, precipitation, or complexation. (2) Biochar enhanced the activity of the antioxidant enzyme system in plants thus improving the Pb tolerance of plants. Statistical analysis methods such as the partial least squares path modeling (PLS-PM) also confirmed the pathways. In a word, clear synergistic effects and pathways could guide the application of biochar and plants in Pb-contaminated soil.
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http://dx.doi.org/10.1016/j.jenvman.2022.115200DOI Listing
August 2022

Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).

Stem Cell Res 2022 07 27;62:102794. Epub 2022 Apr 27.

Research Institute of Neuromuscular and Neurodegenerative Diseases and Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, People's Republic of China; Mitochondrial Medicine Laboratory, Qilu Hospital (Qingdao), Shandong University, Qingdao 266035, Shandong, People's Republic of China; Brain Science Research Institute, Shandong University, Jinan 250012, Shandong, People's Republic of China. Electronic address:

Becker muscular dystrophy (BMD), an X-linked recessive disorder caused of mutation in the dystrophin gene, is characterized by progressive muscle degeneration and proximal muscle weakness. We generated a human induced pluripotent stem cell (hiPSC) line from the fibroblasts isolated from patient with BMD by non-integrating reprogramming methods. The iPSC line highly expresses pluripotency markers, displays the normal karyotype and is able to differentiate into the three germ layers in vitro. The iPSC line will be a useful tool to study the pathogenesis of BMD and for drug screening.
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http://dx.doi.org/10.1016/j.scr.2022.102794DOI Listing
July 2022

Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb.

Int J Anal Chem 2022 26;2022:7067245. Epub 2022 Apr 26.

School of Pharmacy, Linyi University, Linyi 276000, China.

In this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL for both rhein and aloe-emodin. By contrast, the cutoff value for emodin was 2,000 ng mL. No competitive inhibition was observed up to 5,000 ng mL of physcion, chrysophanol, sennoside A, sennoside B, or rhaponticin. Semiquantitative analyses of the total contents of rhein and aloe-emodin in raw drug materials via our colloidal gold immunochromatographic strips produced results agreeable with those determined by HPLC. Taken together, our findings suggest that the implementation of our colloidal gold immunochromatographic strips provides a rapid one-step method for estimating the total contents of rhein and aloe-emodin, which may represent a powerful tool for quality control of rhubarb.
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http://dx.doi.org/10.1155/2022/7067245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064498PMC
April 2022

Rice husk biochar modified-CuCoO as an efficient peroxymonosulfate activator for non-radical degradation of organic pollutants from aqueous environment.

RSC Adv 2021 Dec 10;11(62):39467-39475. Epub 2021 Dec 10.

College of Biological, Chemical Sciences and Engineering, Jiaxing University Jiaxing 314001 Zhejiang PR China

A series of rice husk biochar (RHBC) modified bimetallic oxides were prepared using a simple pyrolysis method to activate peroxymonosulfate (PMS) for the degradation of acid orange G (OG). The results demonstrated that 50 mg L OG was completely decomposed by 1 mM PMS activated with 100 mg L RHBC-CuCoO within 15 min at initial pH 3.4. The OG degradation rate constant of RHBC-CuCoO/PMS (0.95 × 10 min) was five times greater than that of CuCoO/PMS (0.19 × 10 min), suggesting that the introduction of RHBC significantly improved the activity of bimetallic oxides. The effects of the initial pH, catalyst dosage, PMS concentration and reaction temperature on OG removal were also studied. The degradation products of OG were analysed using a gas chromatography-mass spectrometer (GC-MS). Electron paramagnetic resonance (EPR) and quenching experiments showed that singlet oxygen (O) was the main active species. The RHBC-CuCoO/PMS oxidation system is not only unaffected by inorganic anions (Cl, NO , HCO ) and humic acid (HA), but also could remove other typical pollutants of acetaminophen (ACT), sulfathiazole (STZ), rhodamine B (RhB), and bisphenol A (BPA). These findings show that RHBC-CuCoO has great potential for practical applications in the removal of typical organic pollutants.
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http://dx.doi.org/10.1039/d1ra06914dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044812PMC
December 2021

Bilateral Oophorectomy and the Risk of Breast Cancer in BRCA1 Mutation Carriers: A Reappraisal.

Cancer Epidemiol Biomarkers Prev 2022 Jul;31(7):1351-1358

Women's College Research Institute, Women's College Hospital, Toronto, Ontario, Canada.

Background: The lack of consensus on whether bilateral oophorectomy impacts risk of developing breast cancer among BRCA1 mutation carriers might be attributed to various biases, specifically, cancer-induced testing bias due to inclusion of prevalent cases. We conducted two complementary matched case-control analyses to evaluate the association of oophorectomy and BRCA1 breast cancer.

Methods: A research questionnaire was administered every two years to collect information on exposures and disease. In the first analysis, we limited the study to prevalent breast cancer cases (diagnosed prior to study entry; n = 2,962) who were matched to controls on year of birth and country of residence (n = 4,358). In the second approach, we limited to 330 incident cases (diagnosed in the follow-up period) and 1,548 matched controls. Conditional logistic regression was used to estimate the adjusted odds ratios (OR) and 95% confidence intervals (CI) of invasive breast cancer.

Results: In the first approach, there was a significant inverse association between oophorectomy and the risk of developing breast cancer [OR = 0.43; 95% confidence interval (CI), 0.34-0.55; P < 00001]. In the second approach, there was no association between oophorectomy and risk (OR = 1.21; 95% CI, 0.87-1.70; P = 0.26).

Conclusions: The inclusion of women with a personal history of breast cancer prior to ascertainment likely impacts upon the association of oophorectomy and BRCA1 breast cancer risk.

Impact: Oophorectomy is unlikely a determinant of breast cancer risk in BRCA1 mutation carriers but should be offered at age 35 to reduce the risk of ovarian and fallopian tube cancer.
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http://dx.doi.org/10.1158/1055-9965.EPI-21-1196DOI Listing
July 2022
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