Publications by authors named "Ping Song"

451 Publications

HSP70-3 Interacts with Phospholipase Dδ and Participates in Heat Stress Defense.

Plant Physiol 2020 Dec 29. Epub 2020 Dec 29.

College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China.

Heat shock proteins (HSPs) function as molecular chaperones and are key components responsible for protein folding, assembly, translocation, and degradation under stress conditions. However, little is known about how HSPs stabilize proteins and membranes in response to different hormonal or environmental cues in plants. Here, we combined molecular, biochemical, and genetic approaches to elucidate the involvement of cytosolic HSP70-3 in plant stress responses and the interplay between HSP70-3 and plasma membrane (PM)-localized phospholipase Dδ (PLDδ) in Arabidopsis (Arabidopsis thaliana). Analysis using pull-down, coimmunoprecipitation, and bimolecular fluorescence complementation revealed that HSP70-3 specifically interacted with PLDδ. HSP70-3 bound to microtubules, such that it stabilized cortical microtubules upon heat stress. We also showed that heat shock induced recruitment of HSP70-3 to the PM, where HSP70-3 inhibited PLDδ activity to mediate microtubule reorganization, phospholipid metabolism, and plant thermotolerance, and this process depended on the HSP70-3-PLDδ interaction. Our results suggest a model whereby the interplay between HSP70-3 and PLDδ facilitates the re-establishment of cellular homeostasis during plant responses to external stresses and reveal a regulatory mechanism in regulating membrane lipid metabolism.
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http://dx.doi.org/10.1093/plphys/kiaa083DOI Listing
December 2020

Clinical Characteristics of Chronic Lung Abscess Associated with Diagnosed Using Metagenomic Next-Generation Sequencing.

Infect Drug Resist 2021 23;14:1191-1198. Epub 2021 Mar 23.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, People's Republic of China.

Purpose: () is a Gram-positive anaerobic bacterium distributed in the oral cavity, with a potential to become pathogenic causing lung abscess. Due to the lack of specificity of symptoms and the difficulty in culture, the diagnosis of lung abscess associated with is delayed. It is essential to elucidate the clinical characteristics of lung abscess associated with .

Methods: From January 2019 to July 2020, five patients with chronic lung abscess associated with diagnosed by pathological biopsy and metagenomic next-generation sequencing (mNGS) were analyzed in this retrospective study.

Results: Among the five patients, four had a history of smoking, three had periodontitis, and two had a history of drinking. The average course of the disease was 6.5 months. High-density flake-like or mass shadows with irregular boundaries were observed in the chest computed tomography (CT) images of the five patients, and liquefactive necrosis was detected in the middle of the lesions; however, no gas-liquid plane or cavity was noted, making it difficult to distinguish a lung cancer. The pathological biopsy of the five patients showed chronic inflammation of lung tissue, and was detected by mNGS in the biopsy or bronchoalveolar lavage fluid samples. Two patients were treated with amoxicillin-clavulanate, two had metronidazole, and one had moxifloxacin. Among them, four recovered after receiving antibiotic treatment, and the remaining one underwent surgical resection due to poor antibiotic treatment effect.

Conclusion: Chronic lung abscess associated with , common in elderly male smokers with poor oral hygiene, is often diagnosed in a delayed manner and misdiagnosed as lung cancer. The mNGS technology is beneficial to the rapid determination of
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http://dx.doi.org/10.2147/IDR.S304569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001108PMC
March 2021

De novo synthesis of pH-responsive, self-assembled, and targeted polypeptide nano-micelles for enhanced delivery of doxorubicin.

Nanotechnology 2021 Mar 12. Epub 2021 Mar 12.

Anhui Polytechnic University, Wuhu, Anhui, CHINA.

Doxorubicin (DOX) is a commonly used anticancer drug, but is inefficient as a therapeutic due to a lack of targeting. Peptide-tuned self-assembly of DOX offers a strategy to improve targeting for greater efficacy. In this work, we designed and prepared an amphiphilic tumor cell targeting peptide, P14 (AAAAFFFHHHGRGD), able to encapsulate DOX by self-assembly to form tumor cell targeting and pH-sensitive nano-micelles. The results showed that a critical P14-micelle concentration of 1.758 mg/L and an average particle size of micelles of 121.64 nm, with entrapment and drug loading efficiencies of 28.02% ± 1.35% and 12.06% ± 0.59%, respectively. The prepared micelles can release 73.52 ± 1.27% DOX within 24 hours in pH 4.5 medium, and the drug cumulative release profile of micelle can be described by first-order model. Compared with free DOX, the micelles exhibited increased ability to inhibit tumor cell growth and cause tumor apoptosis in vitro, with IC50 values of DOX and P14-DOX micelles against human breast cancer cells (MCF-7) of 0.91 ± 0.07 and 0.75 ± 0.06 μg/mL, respectively, and cellular apoptotic rates of DOX and P14-DOX micelles of 70.3% and 42.4%, respectively. Cellular uptake experiments revealed high concentrations of micelles around and inside MCF-7 cells, demonstrating that micelles can target tumor cells. These results indicate the excellent potential for the application of this amphiphilic peptide as a carrier for small-molecule drugs and suggest a strategy for the design of effective anti-tumor drugs.
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http://dx.doi.org/10.1088/1361-6528/abee49DOI Listing
March 2021

The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism.

Int J Nanomedicine 2021 5;16:1849-1867. Epub 2021 Mar 5.

School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People's Republic of China.

Background: With the development of bacterial resistance, the range of effective antibiotics is increasingly becoming more limited. The effective use of nanoscale antimicrobial peptides (AP) in therapeutic and diagnostic methods is a strategy for new antibiotics.

Methods: Combining both AP and cadmium selenide (CdSe) into a composite material may result in a reagent with novel properties, such as enhanced antibacterial activity, fluorescence and favorable stability in aqueous solution.

Results: AP-loaded CdSe NPs (AP-CdSe NPs) showed strong antibacterial activity against multidrug-resistant (MDR) () and () in vitro and in vivo. Colony-forming unit (CFU) and minimum inhibitory concentration (MIC) assays showed that AP-CdSe NPs have highly effective antibacterial activity. The quantitative analysis of apoptosis by flow cytometry analysis further confirmed that MDR and treated with AP-CdSe NPs had death rates of 98.76% and 99.13%, respectively. Also, AP-CdSe NPs was found to inhibit bacterial activity in an in vivo bacteremia model in mice infected with . In addition, the antibacterial mechanism of AP-CdSe NPs was determined by RNA sequencing analysis. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed the molecular mechanism of the antibacterial effect of AP-CdSe NPs. Importantly, histopathology analysis, and hematological toxicity analysis indicated that AP-CdSe NPs had few side effects.

Conclusion: These results demonstrate that AP loaded on CdSe NPs had a higher water solubility, bioavailability and antibacterial effect compared with raw AP. This study reports findings that are helpful for the design and development of antibacterial treatment strategies based on AP.
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http://dx.doi.org/10.2147/IJN.S295928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943780PMC
March 2021

Perforator-Plus Flaps in Lower Extremity Reconstruction.

Clin Plast Surg 2021 Apr 22;48(2):183-192. Epub 2021 Jan 22.

Division of Plastic Surgery, Department of Surgery, University of California Davis Medical Center, University of California at Davis, 2335 Stockton Boulevard, Room 6008, Sacramento, CA 95817, USA. Electronic address:

The perforator-plus flap is a new concept for lower extremity reconstruction. It combines a perforator flap with a traditional skin rotation flap. It can be another option for lower extremity soft tissue defects since the flap has an augmented blood supply. The ability to detect cutaneous perforators has improved with the Duplex scan imaging technique. These advances have made the perforator-plus flap a viable option for soft tissue reconstruction. It can be versatile and reduces donor site morbidity; it is technically simple to perform and faster than traditional free flaps. It achieves durable soft tissue coverage for lower extremity wounds.
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http://dx.doi.org/10.1016/j.cps.2020.12.003DOI Listing
April 2021

Long non-coding RNA VPS9D1-AS1 facilitates cell proliferation, migration and stemness in hepatocellular carcinoma.

Cancer Cell Int 2021 Feb 24;21(1):131. Epub 2021 Feb 24.

Department of Hepatopancreatobiliary Surgery, The First Hospital of Jilin University, 71 Xinmin Street, Changchun, Jilin, China.

Background: Hepatocellular carcinoma (HCC) is a common cancer leading to high morbidity and mortality in worldwide. Previous studies revealed that SEC61 translocon alpha 1 subunit1 (SEC61A) can act as an oncogene in colon adenocarcinoma. However, the functions and molecular mechanism associated with HCC progression remain to be explored. This study aimed at exploring the role of SEC61A1 in HCC progression.

Methods: EdU assay and colony formation assay were applied to assess cell proliferation. The migratory ability of transfected HCC cells was evaluated by transwell migration assay. Sphere formation assay was used to detect the stemneess of HCC cells. Bioinformatics analysis tools and mechanism experiments were used to predict and analyze the potential molecular mechanism associated with the upregulation of SEC61A1 in HCC cells.

Results: Up-regulated SEC61A1 facilitated cell proliferation, migration and stemness in HCC cells. MiR-491-5p negatively regulated SEC61A1 and inhibited HCC cell proliferation and migration by targeting SEC61A1. VPS9D1 antisense RNA 1 (VPS9D1-AS1) could up-regulate SEC61A1 through sponging miR-491-5p. Early growth response 1 (EGR1) was identified as the upstream transcriptional activator for both SEC61A1 and VPS9D1-AS1.

Conclusions: Our study unveiled a novel molecular pathway facilitating HCC cell proliferation, migration and stemness, which may shed new insight into HCC treatment.
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http://dx.doi.org/10.1186/s12935-020-01741-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903704PMC
February 2021

The regulation of protein translation and its implications for cancer.

Signal Transduct Target Ther 2021 Feb 18;6(1):68. Epub 2021 Feb 18.

Department of Medical Oncology, Cancer Institute of Zhejiang University, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

In addition to the deregulation of gene transcriptions and post-translational protein modifications, the aberrant translation from mRNAs to proteins plays an important role in the pathogenesis of various cancers. Targeting mRNA translation are expected to become potential approaches for anticancer treatments. Protein translation is affected by many factors including translation initiation factors and RNA-binding proteins. Recently, modifications of mRNAs mainly N6-methyladenine (mA) modification and noncoding RNAs, such as microRNAs and long noncoding RNAs are involved. In this review, we generally summarized the recent advances on the regulation of protein translation by the interplay between mRNA modifications and ncRNAs. By doing so, we hope this review could offer some hints for the development of novel approaches in precision therapy of human cancers.
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http://dx.doi.org/10.1038/s41392-020-00444-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889628PMC
February 2021

Increased grey matter volume and associated resting-state functional connectivity in chronic spontaneous urticaria: A structural and functional MRI study.

J Neuroradiol 2021 Feb 4. Epub 2021 Feb 4.

Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA; Harvard Medical School, Boston, MA 02125, USA.

Background And Purpose: Chronic itch is one of the most common irritating sensations, yet its mechanisms have not been fully elucidated. Although some studies have revealed relationships between itching and brain function, the structural changes in the brain induced by chronic itching, such as those accompanying chronic spontaneous urticaria (CSU), remain unclear. In this study, we aimed to explore the potential changes in brain structure and the associated functional circuitry in CSU patients to generate insights to aid chronic itch management.

Methods: Forty CSU patients and forty healthy controls (HCs) were recruited. Seven-day urticaria activity score (UAS7) values were collected to evaluate clinical symptoms. Voxel-based morphometry (VBM) and seed-based resting-state functional connectivity (rs-FC) analysis were used to assess structural changes in the brain and associated changes in functional circuitry.

Results: Compared with HCs, CSU patients had significantly increased grey matter (GM) volume in the right premotor cortex, left fusiform cortex, and cerebellum. UAS7 values were positively associated with GM volume in the left fusiform cortex. In CSU patients relative to HCs, the left fusiform cortex as extracted by VBM analysis demonstrated decreased functional connectivity with the right orbitofrontal cortex, medial prefrontal cortex (mPFC), premotor cortex, primary motor cortex (MI), and cerebellum and increased functional connectivity with the right posterior insular cortex, primary somatosensory cortex (SI), and secondary somatosensory cortex (SII). The left cerebellum as extracted from VBM analysis demonstrated decreased functional connectivity with the right supplementary motor area (SMA) and MI in CSU patients relative to HCs.

Conclusions: Our findings indicate that patients suffering from chronic itching conditions, such as CSU, are likely to demonstrate altered GM volume in some brain regions. These changes may affect not only the sensorimotor area but also brain regions associated with cognitive function.
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http://dx.doi.org/10.1016/j.neurad.2021.01.011DOI Listing
February 2021

A Prediction Nomogram for Acute Kidney Injury in Very-Low-Birth-Weight Infants: A Retrospective Study.

Front Pediatr 2020 15;8:575097. Epub 2021 Jan 15.

Department of Nephrology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.

Acute kidney injury (AKI) is recognized as an independent predictor for mortality in very-low-birth-weight (VLBW) infants and is reported to have a high incidence. In this study, we sought to identify the predictors for AKI in VLBW infants and thereby develop a prediction nomogram for the early detection and management of VLBW infants at high risk of developing AKI. We designed a retrospective study wherein we investigated the baseline hospitalization data of VLBW infants treated at our hospital between January 2012 and October 2018. Independent predictors of AKI in VLBW infants, as identified by multivariate logistic regression, were incorporated into a model. Hosmer-Lemeshow test was used to test the goodness of fit of the model, and a receiver operating characteristic (ROC) curve was plotted to assess the discriminative ability of the model. The model was internally validated using the 10-fold cross-validation method. A nomogram was plotted to predict the risk of AKI in VLBW infants on the basis of the results of multivariate logistic regression analysis. We investigated the data of 604 VLBW infants, of which 144 (23.8%) developed AKI; in 111 (77.1%) of these infants, AKI occurred within 7 days of birth. Multivariate logistic regression analysis identified the following as predictive factors for AKI in VLBW infants: gestational age, red blood cell count within 3 days of birth, serum calcium concentration within 3 days of birth, maternal age of ≥35 years, and pulmonary arterial hypertension or myocardial injury. Furthermore, the nomogram was found to be effective in estimating the risk of AKI in VLBW infants, with an area under the curve (AUC) of 0.794 [95% confidence interval (CI): 0.754-0.834; < 0.001]. Internal validation done by cross-validation showed that the average AUC was 0.788. The nomogram developed in this study was found to be sensitive and specific for the preoperative prediction of AKI in VLBW infants, as per the Kidney Disease: Improving Global Outcomes (KDIGO) criteria modified for neonates.
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http://dx.doi.org/10.3389/fped.2020.575097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844316PMC
January 2021

Advancing the Specialty of Plastic Surgery While Helping and Supporting Other Surgical Services at an Academic Medical Center.

Ann Plast Surg 2021 Jan 28. Epub 2021 Jan 28.

From the Division of Plastic Surgery, Department of Surgery, University of California, Davis, Sacramento, CA.

Abstract: While helping and supporting other surgical services at our academic medical center, we noticed that many clinical problems presented to our plastic surgery team are truly unique and challenging. Many of the necessary reconstructive procedures, performed by the senior author, had never been published in plastic surgery textbooks or the literature before. Because of the plastic surgeon's creative problem-solving ability, we use reconstructive treatment plans that also innovate and advance the field of plastic surgery. In this case series, we share our experience with creative solutions for the management of novel and challenging clinical problems faced by our plastic surgery service. The resultant and effective solutions for each challenging case have all been published in reputable plastic surgery journals by the senior author. Once published, our inventive reconstructive solutions can be harnessed by other plastic surgeons and trainees and new standardized techniques or solutions for these unique and challenging clinical problems can be established. Ultimately, plastic surgeons can evolve their specialty through helping and supporting other surgical services at an academic medical center by establishing innovative solutions for ever arising challenging clinical problems. Once again, the plastic surgery team in a tertiary hospital supports many surgical services so that more complex surgical procedures can be performed safely and complications from other surgical services can be managed successfully. Therefore, adequate support for a plastic surgery service, by the hospital or department, is critical in developing a strong plastic surgery program at an academic medical center.
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http://dx.doi.org/10.1097/SAP.0000000000002728DOI Listing
January 2021

Clonal integration and phosphorus management under light heterogeneity facilitate the growth and diversity of understory vegetation and soil fungal communities.

Sci Total Environ 2021 May 25;767:144322. Epub 2020 Dec 25.

College of Horticulture and Forestry Sciences / Hubei Engineering Technology Research Center for Forestry Information, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

The spatial heterogeneity of light and nutrient deficiency occurs in many forest understories. Proper fertilization management of unhealthy forests can benefit forest understory diversity and improve the stability of degraded soil; and clonal integration is a major advantage of resource sharing for many forest understory vegetation, such as pteridophytes. In this study, we tested whether understory soil fertilization and clonal integration under light heterogeneity were able to increase the performance and diversity of understory vegetation and soil microbial communities in nature. Field experiments-with or without phosphorus (P) addition, with intact or severed rhizome, and under homogeneous or heterogeneous light environments-were conducted in the understory of a typical evergreen forest in southeast China. Light heterogeneity, P addition and clonal integration promoted the growth, diversity and evenness of ferns and soil microbial biomass C, N and P (MBC, MBN and MBP) at both experimental plot and patch level. They also increased Chao1 richness and Shannon diversity of soil fungal communities at patch level, especially in the high light patches with P addition. The positive effects of P addition and clonal integration on the growth and diversity of ferns and soil microbial biomass were greatly increased under heterogeneous light. The positive effects of clonal integration on the growth were the greatest in the heterogeneous high light patches. Moreover, the interactive effect of P addition and clonal integration increased soil MBN and MBP. Clonal integration promoted the increased growth and diversity of ferns and soil MBC in the heterogeneous light environment (9.35%-35.19%), and enhanced soil MBN and MBP in the P addition treatment (9.03%-12.96%). The interactive effect of P addition and clonal integration largely led to the transition of fungal groups from slow-growing oligotrophic types to fast-growing copiotrophic types. Our results show that the interactions between clonal integration and/or P addition under light heterogeneity increase the benefits of ferns in light-rich patches, and further promote integrative performance of ferns and soil microbial communities.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144322DOI Listing
May 2021

An Improved Calibration Method for Photonic Mixer Device Solid-State Array Lidars Based on Electrical Analog Delay.

Sensors (Basel) 2020 Dec 20;20(24). Epub 2020 Dec 20.

Key Laboratory of Biomimetic Robots and Systems (Ministry of Education), Beijing Institute of Technology, Beijing 100081, China.

As a typical application of indirect-time-of-flight (ToF) technology, photonic mixer device (PMD) solid-state array Lidar has gained rapid development in recent years. With the advantages of high resolution, frame rate and accuracy, the equipment is widely used in target recognition, simultaneous localization and mapping (SLAM), industrial inspection, etc. The PMD Lidar is vulnerable to several factors such as ambient light, temperature and the target feature. To eliminate the impact of such factors, a proper calibration is needed. However, the conventional calibration methods need to change several distances in large areas, which result in low efficiency and low accuracy. To address the problems, this paper presents an improved calibration method based on electrical analog delay. The method firstly eliminates the lens distortion using a self-adaptive interpolation algorithm, meanwhile it calibrates the grayscale image using an integral time simulating based method. Then, the grayscale image is used to estimate the parameters of ambient light compensation in depth calibration. Finally, by combining four types of compensation, the method effectively improves the performance of depth calibration. Through several experiments, the proposed method is more adaptive to multiscenes with targets of different reflectivities, which significantly improves the ranging accuracy and adaptability of PMD Lidar.
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http://dx.doi.org/10.3390/s20247329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766223PMC
December 2020

Preparation and application of pH-responsive composite hydrogel beads as potential delivery carrier candidates for controlled release of berberine hydrochloride.

R Soc Open Sci 2020 Nov 4;7(11):200676. Epub 2020 Nov 4.

College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, People's Republic of China.

For improving the effective concentration of berberine hydrochloride (BH) in the gastrointestinal tract, a series of pH-responsive hydrogel beads were prepared based on carboxymethylstarch-g-poly (acrylic acid)/palygorskite/starch/sodium alginate (CMS-g-PAA/PGS/ST/SA) in the present work. The developed hydrogel beads were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TG). Effect of palygorskite (PGS) content on the swelling properties of hydrogel beads and BH cumulative release were discussed. The pH responsiveness of hydrogel beads was also investigated in different media. Results illustrated that swelling of hydrogel beads and BH cumulative release from hydrogel beads were obviously affected by PGS content. The swelling ratio and BH cumulative release of composite hydrogel beads remarkably slowed down with PGS content increasing in the range from 10 to 40 wt%. The composite hydrogel beads were pH-responsive. At pH 7.4, the swelling ratio and BH cumulative release from composite hydrogel beads were the fastest among the dissolution media of pH 1.2, pH 6.8 and pH 7.4. The BH cumulative release from hydrogel beads was related to the swelling and relaxation of composite hydrogel beads and could be fitted better by the Higuchi model. The obtained composite hydrogel beads could be potentially used for the development of BH pharmaceutical dosage forms.
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http://dx.doi.org/10.1098/rsos.200676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735363PMC
November 2020

Discovery of novel 2-aryl-3-sulfonamido-pyridines (HoAns) as microtubule polymerization inhibitors with potent antitumor activities.

Eur J Med Chem 2021 Feb 25;211:113117. Epub 2020 Dec 25.

Department of Chemistry, Zhejiang University, Hangzhou, 310027, PR China. Electronic address:

Microtubules play a vital role in cell mitosis. Drugs targeting taxol or vinca binding site of tubulin have been proved an effective way to against cancer. However, drug resistance and cancer recurrence are inevitable, there is an urgent need to search for new microtubule-targeting agents (MTAs). In our study, a series of novel 2-aryl-3-sulfonamido-pyridines (HoAns) had been designed, synthesized, and evaluated for their antiproliferative activities in vitro and in vivo. Among them, compound HoAn32 exhibited the most potent activity with IC values ranging from 0.170 to 1.193 μM in a panel of cancer cell lines. Mechanism studies indicated that compound HoAn32 bound to the colchicine site of β-tubulin, resulting in colony formation inhibition, G2/M phase cell cycle arrest, cell apoptosis as well as increased the generation of ROS in both RKO and SW620 cells. In addition, compound HoAn32 showed potent anti-vascular activity in vitro. Furthermore, compound HoAn32 also exhibited outstanding antitumor activity in SW620 xenograft tumor models without observable toxic effects, which was more potent than that of ABT-751. In conclusion, our findings suggest that compound HoAn32 may be a promising microtubule destabilizing agent and deserves for further development in cancer therapy.
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http://dx.doi.org/10.1016/j.ejmech.2020.113117DOI Listing
February 2021

Achieving an Optimal Outcome in Immediate Breast Reconstruction.

Ann Plast Surg 2021 03;86(3S Suppl 2):S148-S153

From the Division of Plastic Surgery, University of California, Davis, Sacramento, CA.

Abstract: Performing immediate breast reconstruction requires careful consideration of preoperative, intraoperative, and postoperative factors. One of the fundamental necessities is a well-coordinated multidisciplinary team to achieve successful reconstruction. Clear and effective communication between the oncological surgeon and plastic surgeon is imperative. The treatment plan must take into account any potential neoadjuvant and adjuvant therapies. Surgical planning and incisional approach must be carefully considered to optimize a safe oncologic surgery and ensure a viable reconstructive outcome. Furthermore, there are fundamentals to immediate breast reconstruction that must be met to obtain an aesthetically pleasing and structurally durable breast reconstruction. The goal of this article was to highlight important considerations in surgical planning and execution of immediate breast reconstruction so that an optimal outcome can be accomplished after either autologous or implant-based breast reconstruction.
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http://dx.doi.org/10.1097/SAP.0000000000002640DOI Listing
March 2021

Evidence-based regulations for bioinformatic prediction of allergen cross-reactivity are needed.

Regul Toxicol Pharmacol 2021 Mar 15;120:104841. Epub 2020 Dec 15.

Corteva Agriscience, P.O. Box 30 Newark DE, 19714, USA.

The bioinformatic criteria adopted by regulatory agencies to predict the potential cross reactivity between newly expressed proteins in genetically engineered crops and known allergens involves amino acid identity thresholds and was formulated nearly two decades ago based on the opinion of allergy experts. Over the subsequent years, empirical evidence has been developed indicating that better bioinformatic tools based on amino acid similarity are available to detect real allergen cross-reactive risk while substantially reducing false-positive detections. Although the formulation of safety regulations, in the absence of empirical evidence, may require reliance on expert opinion, such expert opinion should not trump empirical evidence once it becomes available. The failure of regulation to maintain consistency with the best available scientific evidence diminishes its value and creates arbitrary barriers to the use of beneficial technologies by society.
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http://dx.doi.org/10.1016/j.yrtph.2020.104841DOI Listing
March 2021

Cone Photoreceptor Degeneration and Neuroinflammation in the Zebrafish Bardet-Biedl Syndrome 2 () Mutant Does Not Lead to Retinal Regeneration.

Front Cell Dev Biol 2020 26;8:578528. Epub 2020 Nov 26.

Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States.

Bardet-Biedl syndrome (BBS) is a heterogeneous and pleiotropic autosomal recessive disorder characterized by obesity, retinal degeneration, polydactyly, renal dysfunction, and mental retardation. BBS results from defects in primary and sensory cilia. Mutations in 21 genes have been linked to BBS and proteins encoded by 8 of these genes form a multiprotein complex termed the BBSome. Mutations in , a component of the BBSome, result in BBS as well as non-syndromic retinal degeneration in humans and rod degeneration in mice, but the role of BBS2 in cone photoreceptor survival is not clear. We used zebrafish mutants to better understand how loss of leads to photoreceptor degeneration. Zebrafish mutants exhibited impaired visual function as larvae and adult zebrafish underwent progressive cone photoreceptor degeneration. Cone degeneration was accompanied by increased numbers of activated microglia, indicating an inflammatory response. Zebrafish exhibit a robust ability to regenerate lost photoreceptors following retinal damage, yet cone degeneration and inflammation was insufficient to trigger robust Müller cell proliferation. In contrast, high intensity light damage stimulated Müller cell proliferation and photoreceptor regeneration in both wild-type and mutants, although the mutants could only restore cones to pre-damaged densities. In summary, these findings suggest that cone degeneration leads to an inflammatory response in the retina and that BBS2 is necessary for cone survival. The zebrafish mutant also represents an ideal model to identify mechanisms that will enhance retinal regeneration in degenerating diseases.
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http://dx.doi.org/10.3389/fcell.2020.578528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726229PMC
November 2020

Wireless Transmission Method for Large Data Based on Hierarchical Compressed Sensing and Sparse Decomposition.

Sensors (Basel) 2020 Dec 13;20(24). Epub 2020 Dec 13.

The Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, Beijing Institute of Technology, Beijing 100081, China.

With the widespread application of wireless sensor networks, large-scale systems with high sampling rates are becoming more and more common. The amount of original data generated by the wireless sensor network is very large, and transmitting all the original data back to the host wastes network bandwidth and energy. This paper proposes a wireless transmission method for large data based on hierarchical compressed sensing and sparse decomposition. This method includes a hierarchical signal decomposition method based on the same sparse basis and different sparse basis hierarchical compressed sensing method with a mask. Compared with the traditional compressed sensing method, this method reduces the error of signal reconstruction, reduces the amount of calculation during signal reconstruction, and reduces the occupation of hardware resources. We designed comparison experiments between the traditional compressed sensing algorithm and the method proposed in this article. In addition, the experiments' results prove that our proposed method reduces the execution time, as well as the reconstruction error, compared with the traditional compressed sensing algorithm, and it can achieve better reconstruction at a relatively low compression ratio.
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http://dx.doi.org/10.3390/s20247146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764053PMC
December 2020

Isocitrate dehydrogenase 1 from Acinetobacter baummanii (AbIDH1) enzymatic characterization and its regulation by phosphorylation.

Biochimie 2021 Feb 5;181:77-85. Epub 2020 Dec 5.

Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases and Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, Anhui Normal University, Wuhu, 241000, Anhui, China. Electronic address:

Acinetobacter baumannii encodes all enzymes required in the tricarboxylic acid (TCA) cycle and glyoxylate bypass except for isocitrate dehydrogenase kinase/phosphatase (IDHKP), which can phosphorylate isocitrate dehydrogenase (IDH) at a substrate-binding Ser site and control the carbon flux in enterobacteria, such as Escherichia coli. The potential kinase was not successfully pulled down from A. baumannii cell lyase; therefore, whether the IDH 1 from A. baumannii (AbIDH1) can be phosphorylated to regulate intracellular carbon flux has not been clarified. Herein, the AbIDH1 gene was cloned, the encoded protein was expressed and purified to homogeneity, and phosphorylation and enzyme kinetics were evaluated in vitro. Gel filtration and SDS-PAGE analyses showed that AbIDH1 is an 83.5 kDa homodimer in solution. The kinetics showed that AbIDH1 is a fully active NADP-dependent enzyme. The Michaelis constant K is 46.6 (Mn) and 18.1 μM (Mg) for NADP and 50.5 (Mn) and 65.4 μM (Mg) for the substrate isocitrate. Phosphorylation experiments in vitro indicated that AbIDH1 is a substrate for E. coli IDHKP. The activity of AbIDH1 treated with E. coli IDHKP immediately decreased by 80% within 9 min. Mass spectrometry indicated that the conserved Ser113 of AbIDH1 is phosphorylated. Continuous phosphorylation-mimicking mutants (Ser113Glu and Ser113Asp) lack almost all enzymatic activity. Side-chain mutations at Ser113 (Ser113Thr, Ser113Ala, Ser113Gly and Ser113Tyr) remarkably reduce the enzymatic activity. Understanding the potential of AbIDH1 phosphorylation enables further investigations of the AbIDH1 physiological functions in A. baumannii.
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http://dx.doi.org/10.1016/j.biochi.2020.12.001DOI Listing
February 2021

The antihypertension effect of hydrogen sulfide (HS) is induced by activating VEGFR2 signaling pathway.

Life Sci 2021 Feb 27;267:118831. Epub 2020 Nov 27.

College of Pharmacy, Xinxiang Medical University, Xinxiang, China; Henan International Joint Laboratory of Cardiovascular Remodeling and Drug Intervention, Xinxiang 453003, China; Xinxiang Key Laboratory of Vascular Remodeling Intervention and Molecular Targeted Therapy Drug Development, Xinxiang 453003, China. Electronic address:

Aims: Previous studies demonstrated that HS has an antihypertension effect on hypertension, but the mechanism involved is unclear until now. The aim of the study is to elucidate the effect of HS on PH and the mechanism involved.

Main Methods: In this study, GYY4137 (a HS donor) were administered to spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) by intraperitoneally injection daily for consecutive 14 days. Systolic blood pressure (SBP), endothelial-dependent relaxation (EDR), plasma malondialdehyde (MDA), superoxide dismutase (SOD), and HS levels were measured. Human umbilical vein endothelial cells (HUVECs) were also used to elucidate the mechanism involved in the protect effect of HS on the injured vessels.

Key Findings: Our results showed that GYY4137 normalized the SBP (P < 0.0001), increased EDR (P < 0.01), reduced oxidative stress (increased the content of SOD and reduced the content of MDA) of SHR. Meanwhile, GYY4137 could promote the proliferation (P < 0.01) and migration (P < 0.01) of HUVECs, increase the expression of endothelial NO synthase (eNOS) and Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) both in SHR and HUVECs treated with GYY4137. In addition to the above results, the PIP3/Akt signaling pathway was activated and the expression of caspase 3 was increased by GYY4137. However, all the above effects of GYY4137 were blocked by ZD6474 (a VEGFR2 inhibitor).

Significance: GYY4137 had a hypotensive and vascular protect effect on PH. This effect might be mediated through upregulating the expression of VEGFR2, which subsequently alleviating oxidant-provoked vascular endothelial dysfunction, and promoting the proliferation and migration of endothelial cells in SHR.
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http://dx.doi.org/10.1016/j.lfs.2020.118831DOI Listing
February 2021

Carbapenemase OXA-423: A Novel OXA-23 Variant in .

Infect Drug Resist 2020 10;13:4069-4075. Epub 2020 Nov 10.

Department of Clinical Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu, People's Republic of China.

Purpose: A novel variant of OXA-23, named OXA-423, was identified in an clinical isolate. The aim of this study was to analyse the resistance phenotype of OXA-423.

Methods: The strain WY-0713 was isolated from an intensive care unit patient. PCR was used to detect the -like genes. Amplifying, cloning and sequencing were performed for the complete -like. The novel and its ancestor were cloned into the expression vector pET-28b(+), and transformed into Rosetta (DE3) for antibiotic susceptibility testing. SDS-PAGE, modified Hodge test and CarbaNP test were used for detecting the expression of OXA-423 and OXA-23.

Results: PCR screening of WY-0713 was positive for -like genes. Sequencing of the PCR product identified a novel  named which encoding  OXA-423. OXA-423 differed from OXA-23 by a crucial amino acid substitution (Val128Ala). The V128A substitution was located at the conserved active-site motifs SAV of OXA-23. Antibiotic susceptibility testing performed using isogenic showed that the MICs of Rosetta (pET-OXA-423) for penicillins and carbapenems were lower (reduced MICs 4-fold to 16-fold) than that of Rosetta (pET-OXA-23). The MICs of cefotaxime, ceftazidime and aztreonam for both transformants remained the same as the acceptor strain. Moreover, OXA-423 was slightly inhibited by sulbactam, clavulanic acid and tazobactam. SDS-PAGE analysis showed that OXA-423 and OXA-23 were conspicuously expressed. Modified Hodge test and CarbaNP test were positive demonstrated both of them were functional.

Conclusion: OXA-423, the first report of an amino acid substitution located at conserved active-site motifs of OXA-23, conferred lower MIC values of penicillins and carbapenems as compared with OXA-23, while without affecting the resistance profiles of expanded-spectrum cephalosporins and aztreonam.
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http://dx.doi.org/10.2147/IDR.S277364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666985PMC
November 2020

Positive RT-PCR Test Results in 420 Patients Recovered From COVID-19 in Wuhan: An Observational Study.

Front Pharmacol 2020 7;11:549117. Epub 2020 Oct 7.

Departments of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Objective: During the follow-up of patients recovered from coronavirus disease 2019 (COVID-19) in the quarantine and observation period, some of the cured patients showed positive results again. The recurrent positive RT-PCR test results drew widespread concern. We observed a certain number of cured COVID-19 patients with positive RT-PCR test results and try to analyze the factors that caused the phenomenon.

Methods: We conducted an observational study in COVID-19 patients discharged from 6 rehabilitation stations in Wuhan, China. All observed subjects met the criteria for hospital discharge and were in quarantine. Data regarding age, sex, body mass index (BMI), course of disease, comorbidity, smoking status and alcohol consumption, symptoms in and out of quarantine, and intervention were collected from the subjects' medical records and descriptively analyzed. The main outcome of this study was the RT-PCR test result of the observed subjects at the end of quarantine (negative or positive). Logistic regression analysis was used to identify the influencing factors related to recurrent positive RT-PCR test results.

Results: In this observational study, 420 observed subjects recovered from COVID-19 were included. The median age was 56 years, 63.6% of the subjects were above 50 years old, and 50.7% (213/420) were female. The most common comorbidities were hypertension [26.4% (111/420)], hyperlipidemia [10.7% (45/420)], and diabetes [10.5% (44/420)]. 54.8% (230/420) manifested one or more symptoms at the beginning of the observation period, the most common symptoms were cough [27.6% (116/420)], shortness of breath 23.8% (100/420)], and fatigue [16.2% (68/420)], with fever rare [2.6% (11/420)]. A total of 325 subjects were exposed to comprehensive intervention; 95 subjects were absence of intervention. The recurrence rate of positive RT-PCR test results with comprehensive intervention was 2.8% (9/325), and that with no intervention was 15.8% (15/95). The results of logistic regression analysis showed that after adjusted for factors such as age, sex, and comorbidity and found out that comprehensive intervention was correlated with the recurrent positive RT-PCR test results. There was appreciably less recurrence in the comprehensive intervention group.

Conclusions: The factors related to positive RT-PCR test results in observed subjects recovered from COVID-19 were age, comorbidity, and comprehensive intervention, among which comprehensive intervention might be a protective factor.

Clinical Trial Registration: Chictr.org.cn, identifier ChiCTR2000030747.
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http://dx.doi.org/10.3389/fphar.2020.549117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577046PMC
October 2020

Citronellal ameliorates doxorubicin-induced hepatotoxicity via antioxidative stress, antiapoptosis, and proangiogenesis in rats.

J Biochem Mol Toxicol 2021 Feb 13;35(2):e22639. Epub 2020 Oct 13.

Henan International Joint Laboratory of Cardiovascular Remodeling and Drug Intervention, Xinxiang, China.

Doxorubicin (DOX) is a very effective broad-spectrum anticancer drug, yet its clinical application is badly restricted due to its serious side effects. Citronellal (CT), a specialized metabolite of plants found in Cymbopogon spp., is proved to exhibit many beneficial properties. In the current study, we intended to investigate the effect of CT on DOX-induced hepatotoxicity in rats. Rats were treated with CT (200 mg/kg b.w./day orally), and given DOX (2.5 mg/kg b.w./week, intraperitoneally) to induce hepatotoxicity for six consecutive weeks. The results showed that CT administration could attenuate the DOX-induced pathological changes of liver tissues and ameliorated the inappropriate alteration of liver function biomarkers (serum glutamic aspartate aminotransferase, glutamic pyruvic transaminase, and albumin) in serum and oxidative stress parameters (malondialdehyde, superoxide dismutase, and reduced glutathione) in the liver. Moreover, CT mitigated the Bax/Bcl-2 ratio and caspase-3 expression to inhibit cell apoptosis. Further study indicated that CT therapy could enhance the protein levels of p-PI3K, p-Akt, and CD31 in the liver. These results demonstrate that CT can ameliorate DOX-induced hepatotoxicity in rats mediated by antioxidative stress, antiapoptosis, and proangiogenesis.
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http://dx.doi.org/10.1002/jbt.22639DOI Listing
February 2021

Corrigendum: Construction of SARS-CoV-2 Virus-Like Particles by Mammalian Expression System.

Front Bioeng Biotechnol 2020 9;8:1026. Epub 2020 Sep 9.

Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China.

[This corrects the article DOI: 10.3389/fbioe.2020.00862.].
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http://dx.doi.org/10.3389/fbioe.2020.01026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509488PMC
September 2020

Li-Doping Effect on Characteristics of ZnO Thin Films Resistive Random Access Memory.

Micromachines (Basel) 2020 Sep 24;11(10). Epub 2020 Sep 24.

Key Laboratory of Electronics Engineering, College of Heilongjiang Province, Heilongjiang University, Harbin 150080, China.

In this study, a Pt/Ag/LZO/Pt resistive random access memory (RRAM), doped by different Li-doping concentrations was designed and fabricated by using a magnetron sputtering method. To determine how the Li-doping concentration affects the crystal lattice structure in the composite ZnO thin films, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) tests were carried out. The resistive switching behaviors of the resulting Pt/Ag/LZO/Pt devices, with different Li-doping contents, were studied under direct current (DC) and pulse voltages. The experimental results showed that compared with the devices doped with Li-8% and -10%, the ZnO based RRAM device doped by 5% Li-doping presented stable bipolar resistive switching behaviors with DC voltage, including a low switching voltage (<1.0 V), a high endurance (>10 cycles), long retention time (>10 s), and a large resistive switching window. In addition, quick switching between a high-resistance state (HRS) and a low-resistance state (LRS) was achieved at a pulse voltage. To investigate the resistive switching mechanism of the device, a conduction model was installed based on Ag conducting filament transmission. The study of the resulting Pt/Ag/LZO/Pt devices makes it possible to further improve the performance of RRAM devices.
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http://dx.doi.org/10.3390/mi11100889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599982PMC
September 2020

Synthesis of structurally defined chondroitin sulfate: Paving the way to the structure-activity relationship studies.

Carbohydr Polym 2020 Nov 24;248:116796. Epub 2020 Jul 24.

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China. Electronic address:

Chondroitin sulfate (CS) is one of the major and widespread glycosaminoglycans, a family of structurally complex, linear, anionic hetero-co-polysaccharides. CS plays a vital role in various normal physiological and pathological processes, thus, showing varieties of biological activities, such as anti-oxidation, anti-atherosclerosis, anti-thrombosis, and insignificant immunogenicity. However, the heterogeneity of the naturally occurring CS potentially leads to function unspecific and limits further structure-activity relationship studies. Therefore, the synthesis of CS with well-defined and uniform chain lengths is of major interest for the development of reliable drugs. In this review, we examine the remarkable progress that has been made in the chemical, enzymatic and chemoenzymatic synthesis of CS and its derivatives, providing a broad spectrum of options to access CS of well controlled chain lengths.
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http://dx.doi.org/10.1016/j.carbpol.2020.116796DOI Listing
November 2020

Construction of SARS-CoV-2 Virus-Like Particles by Mammalian Expression System.

Front Bioeng Biotechnol 2020 30;8:862. Epub 2020 Jul 30.

Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China.

Virus-like particle (VLP) is a self-assembled nanostructure incorporating key viral structural proteins. VLP resembles molecular and morphological features of authentic viruses but is non-infectious and non-replicating due to lack of genetic materials. Successful applications of VLP has been shown in vaccinological and virological research. As an accessibly safe and relevant substitute of naturally pathogenic viruses, the construction of SARS-CoV-2 VLPs is much in demand in the ongoing fight against 2019 Coronavirus disease (COVID-19) pandemics. In the current study, using mammalian expression system, which is advantageous in maintaining correct protein glycosylation patterns, we efficiently constructed SARS-CoV-2 VLPs. We showed that among four SARS-CoV-2 structural proteins, expression of membrane protein (M) and small envelope protein (E) are essential for efficient formation and release of SARS-CoV-2 VLPs. Moreover, the corona-like structure presented in SARS-CoV-2 VLPs from Vero E6 cells is more stable and unified, as compared to those from HEK-293T cells. Our data demonstrate that SARS-CoV-2 VLPs possess molecular and morphological properties of native virion particles, which endow such VLPs with a promising vaccine candidate and a powerful tool for the research of SARS-CoV-2.
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http://dx.doi.org/10.3389/fbioe.2020.00862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409377PMC
July 2020

β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer.

Mol Cancer 2020 08 24;19(1):129. Epub 2020 Aug 24.

Labortary of Cancer Biology, Key Lab of Biotherapy in Zhejiang, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Heat shock transcription factor1 (HSF1) was overexpressed to promote glutaminolysis and activate mTOR in colorectal cancer (CRC). Here, we investigated the mechanism for cancer-specific overexpression of HSF1.

Methods: HSF1 expression was analyzed by chromatin immunoprecipitation, qRT-PCR, immunohistochemistry staining and immunoblotting. HSF1 translation was explored by polysome profiling and nascent protein analysis. Biotin pulldown and m6A RNA immunoprecipitation were applied to investigate RNA/RNA interaction and m6A modification. The relevance of HSF1 to CRC was analyzed in APC and APC HSF1mice.

Results: HSF1 expression and activity were reduced after the inhibition of WNT/β-catenin signaling by pyrvinium or β-catenin knockdown, but elevated upon its activation by lithium chloride (LiCl) or β-catenin overexpression. There are much less upregulated genes in HSF1-KO MEF treated with LiCl when compared with LiCl-treated WT MEF. HSF1 protein expression was positively correlated with β-catenin expression in cell lines and primary tissues. After β-catenin depletion, HSF1 mRNA translation was impaired, accompanied by the reduction of its m6A modification and the upregulation of miR455-3p, which can interact with 3'-UTR of HSF1 mRNA to repress its translation. Interestingly, inhibition of miR455-3p rescued β-catenin depletion-induced reduction of HSF1 m6A modification and METTL3 interaction. Both the size and number of tumors were significantly reduced in APC mice when HSF1 was genetically knocked-out or chemically inhibited.

Conclusions: β-catenin suppresses miR455-3p generation to stimulate m6A modification and subsequent translation of HSF1 mRNA. HSF1 is important for β-catenin to promote CRC development. Targeting HSF1 could be a potential strategy for the intervention of β-catenin-driven cancers.
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http://dx.doi.org/10.1186/s12943-020-01244-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446108PMC
August 2020

Involvement of Arabidopsis phospholipase D δ in regulation of ROS-mediated microtubule organization and stomatal movement upon heat shock.

J Exp Bot 2020 10;71(20):6555-6570

College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

Reactive oxygen species (ROS) are plant metabolic and signaling molecules involved in responses to various external stresses, but the existence of ROS receptors and how plants respond to ROS remain largely unknown. Here we report that the plasma membrane-localized phospholipase D δ (PLDδ) protein is crucial for sensing heat shock-induced ROS to initiate reorganization of guard cell microtubules in Arabidopsis cotyledons. Heat shock of wild-type Arabidopsis cotyledons stimulated ROS production which disrupted microtubule organization and induced stomatal closure, whereas this process was markedly impaired in pldδ mutants. Moreover, wild-type PLDδ, but not the Arg622-mutated PLDδ, complemented the pldδ phenotypes in heat shock-treated plants. ROS activated PLDδ by oxidizing cysteine residues, an action that was required for its functions in ROS-induced depolymerization of guard cell microtubules, stomatal closure, and plant thermotolerance. Additionally, lipid profiling reveals involvement of microtubule organization in the feedback regulation of glycerolipid metabolism upon heat stress. Together, our findings highlight a potential mechanosensory role for PLDδ in regulating the dynamic organization of microtubules and stomatal movement, as part of the ROS-sensing pathway, during the response to external stresses.
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http://dx.doi.org/10.1093/jxb/eraa359DOI Listing
October 2020

Simultaneously Improved Thermal and Dielectric Performance of Epoxy Composites Containing TiCT Platelet Fillers.

Polymers (Basel) 2020 Jul 19;12(7). Epub 2020 Jul 19.

School of Renewable Energy, North China Electric Power University, Beijing 102206, China.

Polymer composites with enhanced thermal and dielectric properties can be widely used in electric and energy related applications. In this work, epoxy composites have been prepared with TiCT, one of the most studied MXene materials that can be massively produced by direct etching using hydrofluoric acid. The addition of conductive two dimensional TiCT platelet fillers leads to improved but anisotropic thermal conductivity of the composites. The through-plane thermal conductivity reaches 0.583 WmK and the in-plane thermal conductivity reaches 1.29 WmK when filler content is 40 wt% (21.3 vol%), achieving enhancements of 2.92 times and 10.65 times respectively, as compared with epoxy matrix. The dielectric permittivity of epoxy composite is enhanced by a factor of ~2.25 with 40 wt% fillers, and the dielectric losses are within a small value of 0.02. The results prove the effectiveness of TiCT in simultaneously improving thermal and dielectric performance of epoxy composites, and it is deduced that further improvements may be obtained by using TiCT nanoflake fillers.
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http://dx.doi.org/10.3390/polym12071608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408071PMC
July 2020