Publications by authors named "Ping Mo"

19 Publications

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Proposal of Lentzea deserti (Okoro et al. 2010) Nouioui et al. 2018 as a later heterotypic synonym of Lentzea atacamensis (Okoro et al. 2010) Nouioui et al. 2018 and an emended description of Lentzea atacamensis.

PLoS One 2021 4;16(2):e0246533. Epub 2021 Feb 4.

Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, Changsha, Hunan, China.

The taxonomic relationship of Lentzea atacamensis and Lentzea deserti were re-evaluated using comparative genome analysis. The 16S rRNA gene sequence analysis indicated that the type strains of L. atacamensis and L. deserti shared 99.7% sequence similarity. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the genomes of two type strains were 88.6% and 98.8%, respectively, greater than the two recognized thresholds values of 70% dDDH and 95-96% ANI for bacterial species delineation. These results suggested that L. atacamensis and L. deserti should share the same taxonomic position. And this conclusion was further supported by similar phenotypic and chemotaxonomic features between them. Therefore, we propose that L. deserti is a later heterotypic synonym of L. atacamensis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246533PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861442PMC
February 2021

sp. nov., an endophytic actinomycete isolated from the roots of .

Int J Syst Evol Microbiol 2019 Jun 2;71(3). Epub 2021 Feb 2.

School of Life Science, Hunan University of Science and Technology, Xiangtan 411201 Hunan, PR China.

A novel endophytic actinomycete, designated strain Gen 01, was isolated from the roots of and characterized by using a polyphasic approach. The predominant cellular fatty acids were iso-C, summed feature 3, iso H-C, C and iso-C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine inositol, phosphatidylinositol and unidentified phospholipids. The major menaquinone was MK-8 (H). The DNA G+C content of the genome sequence, consisting of 7 177 725 bp, was 74.5 mol%. Phylogenetic analysis of the full-length 16S rRNA gene sequences showed that strain Gen 01 belongs to the genus with the highest sequence similarity to CGMCC 4.1532 (98.9 %) and lower than 98.7 % similarity to other species of the genus with validly published names. The average nucleotide identity and digital DNA-DNAhybridization values between strain Gen 01 and CGMCC 4.1532 were 84.6 and 30.9 %, respectively. Furthermore, the morphological, physiological and biochemical characteristics were sufficient to categorize strain Gen 01 as being distinct from CGMCC 4.1532. Consequently, based on phenotypic and genotypic characteristics, strain Gen 01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gen 01 (=CICC 24820=JCM 33840).
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http://dx.doi.org/10.1099/ijsem.0.004680DOI Listing
June 2019

Retraction Note to: Resibufogenin suppresses tumor growth and inhibits glycolysis in ovarian cancer by modulating PIM1.

Naunyn Schmiedebergs Arch Pharmacol 2021 Mar;394(3):575

Department of Gynecology, Qingdao Hospital of Traditional Chinese Medicine, Renmin Road 4, Qingdao, 266000, Shandong, China.

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http://dx.doi.org/10.1007/s00210-020-02016-8DOI Listing
March 2021

sp. nov. and sp. nov., isolated from the leaves and rhizosphere soil of .

Int J Syst Evol Microbiol 2020 Dec;70(12):6458-6467

College of Forestry, Northwest A &F University, Yangling, 712100 Shaanxi, PR China.

Two novel actinobacteria, designated strains GY16 and T44, were isolated from the leaves and rhizosphere soil of , respectively. A polyphasic approach was used for determining their taxonomic position. Results of 16S rRNA gene sequence analysis indicated that strain GY16 exhibited highest similarities to subsp. CGMCC 4.1593 (98.82 %), KCTC 19241 (98.76 %), NRRL B-16523 (98.69 %), KACC 20186 (98.69 %) and NBRC 13407 (98.69 %), and strain T44 showed 99.2, 99.1, 99.1 and <98.7 % sequence similarities to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and other species, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GY16 formed an independent subclade, which indicated that strain GY16 should belong to a potential novel species; and strain T44 was closely related to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and DSM 40224. However, the multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization values between closely related relatives were far from the species-level thresholds. In addition, phenotypic and chemotaxonomic characteristics further confirmed that strains GY16 and T44 belonged to two distinct species. Based on these results, it is concluded that the isolated strains represent novel species within the genus , for which the names sp. nov. (type strain GY16=CICC 24807=KCTC 49326) and sp. nov. (type strain T44=CICC 24819=JCM 33918) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004556DOI Listing
December 2020

Blade-like structure of graphene oxide sheets decorated with cuprous oxide and silicon carbide nanocomposites as bactericidal materials.

J Colloid Interface Sci 2020 Oct 20;578:698-709. Epub 2020 Jun 20.

Key Laboratory of Clean Chemistry Technology of Guangdong Regular Higher Education Institutions, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address:

In the current work, graphene oxide nanosheets decorated with cuprous oxide nanospheres (GO/CuO) and with silicon carbide nanowires (GO/SiC) were synthesized and controlled for using as blade-like antibacterial agents. Preparation of GO sheets with <2 nm thickness was performed through a modified Hummers approach. Controlled CuO spheres with a mean size of 40 nm were prepared through a wet-chemical approach. For comparative studies, a single step chemical deposition method was used to prepare GO/CuO and GO/SiC nanocomposites for using as antibacterial active materials. The nanomaterials' biological behavior and bacterial-resistance were assessed via selected Gram-negative and gram-positive bacteria and yeast strains. GO/CuO nanocomposite exhibited higher antibacterial activity against different bacterial strains than GO/SiC composite. GO/CuO exhibited average activity index, minimum inhibitory concentration (MIC) values, and viable cell numbers of 1.523, 10.438 µg/mL, and 82.962% for Bacillus subtilis, Brevibacillus brevis and Bacillus thuriginesis), 1.453, 32.00 µg/mL, and 68.418% for Pseudomonas aeruginosa and Escherichia coli) and 68.608% for Candida albicans, respectively. The antimicrobial efficiency of the blade-like GO/CuO was elucidated by scanning electron microscopy through the complete wrapping of the cell membranes and disrupting their shape morphology. GO nanosheets could increase the CuO dispersion in the aqueous solution, prevent their agglomeration, and stabilize its action in aqueous solution with high microbial toxicity.
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http://dx.doi.org/10.1016/j.jcis.2020.06.058DOI Listing
October 2020

Resibufogenin suppresses tumor growth and inhibits glycolysis in ovarian cancer by modulating PIM1.

Naunyn Schmiedebergs Arch Pharmacol 2019 12 10;392(12):1477-1489. Epub 2019 Jul 10.

Department of Gynecology, Qingdao Hospital of Traditional Chinese Medicine, Renmin Road 4, Qingdao, 266000, Shandong, China.

Ovarian cancer is a common human malignancy of the female reproductive system. However, chemotherapy has been proven to have limited effectiveness in a majority of patients. Resibufogenin (RB) is a major active ingredient in cinobufacini, which has been used in the treatment of human malignancies as adjunct agents. This study was designed to examine the anti-cancer effect of RB and the underlying mechanisms in ovarian cancer. Our results showed that RB treatment resulted in cell death, cell cycle arrest, and apoptosis in ovarian cancer cells. The anti-growth and pro-apoptotic effects of RB were also validated in xenograft mice models. Proteomics analysis indicated that RB was able to alter the expressions of several genes, which were involved in the regulation of glycolysis. The suppression effect of RB in the glycolysis pathway of ovarian cancer cells was validated by decreased glucose consumption, lactate production, and extracellular acidification rate (ECAR). We proposed that PIM1 functioned as the key target that mediated the anti-cancer effect of RB against ovarian cancer cells. Our results have revealed that RB downregulated PIM1 in ovarian cancer cells and its downstream genes involved in glycolysis. Moreover, our results indicated that the anti-growth activities and suppressing effect of RB on glycolysis were enhanced significantly by PIM1 knockdown but was attenuated by ectopic PIM1 expression. This provided evidence to support the role of PIM1 in the anti-cancer activities of RB.
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http://dx.doi.org/10.1007/s00210-019-01687-2DOI Listing
December 2019

Non-canonical Opioid Signaling Inhibits Itch Transmission in the Spinal Cord of Mice.

Cell Rep 2018 Apr;23(3):866-877

Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address:

Chronic itch or pruritus is a debilitating disorder that is refractory to conventional anti-histamine treatment. Kappa opioid receptor (KOR) agonists have been used to treat chronic itch, but the underlying mechanism remains elusive. Here, we find that KOR and gastrin-releasing peptide receptor (GRPR) overlap in the spinal cord, and KOR activation attenuated GRPR-mediated histamine-independent acute and chronic itch in mice. Notably, canonical KOR-mediated G signaling is not required for desensitizing GRPR function. In vivo and in vitro studies suggest that KOR activation results in the translocation of Ca-independent protein kinase C (PKC)δ from the cytosol to the plasma membrane, which in turn phosphorylates and inhibits GRPR activity. A blockade of phospholipase C (PLC) in HEK293 cells prevented KOR-agonist-induced PKCδ translocation and GRPR phosphorylation, suggesting a role of PLC signaling in KOR-mediated GRPR desensitization. These data suggest that a KOR-PLC-PKCδ-GRPR signaling pathway in the spinal cord may underlie KOR-agonists-induced anti-pruritus therapies.
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http://dx.doi.org/10.1016/j.celrep.2018.03.087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937707PMC
April 2018

Streptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil.

Int J Syst Evol Microbiol 2018 Jun 12;68(6):1890-1895. Epub 2018 Apr 12.

Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, PR China.

A novel actinomycete isolate, designated strain MK44, was isolated from a Manganese-polluted soil sample collected near Xiangtan Manganese Mine, South Central China and subjected to a polyphasic taxonomic characterization. Comparison of 16S rRNA gene sequences showed that strain MK44 was a member of the genus Streptomyces and most closely related to Streptomyces specialis JCM 16611 (97.9 %) and Streptomyces mayteni JCM 16957 (97.4 %). The DNA-DNA relatedness between strain MK44 and the above two related type species were 30.9±0.3 and 29.9±3.5 %, respectively, values which are far lower than the 70 % threshold for the delineation of a novel prokaryotic species. Furthermore, the results of physiological, biochemical and chemotaxonomic tests allowed further phenotypic differentiation. Therefore, it is concluded that strain MK44 represents a novel species of the genus Streptomyces, for which the name Streptomyces manganisoli sp. nov. is proposed. The type strain is MK44 (=GDMCC 4.137=KCTC 39920).
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http://dx.doi.org/10.1099/ijsem.0.002762DOI Listing
June 2018

Distinct roles of NMB and GRP in itch transmission.

Sci Rep 2017 11 13;7(1):15466. Epub 2017 Nov 13.

Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, 63110, USA.

A key question in our understanding of itch coding mechanisms is whether itch is relayed by dedicated molecular and neuronal pathways. Previous studies suggested that gastrin-releasing peptide (GRP) is an itch-specific neurotransmitter. Neuromedin B (NMB) is a mammalian member of the bombesin family of peptides closely related to GRP, but its role in itch is unclear. Here, we show that itch deficits in mice lacking NMB or GRP are non-redundant and Nmb/Grp double KO (DKO) mice displayed additive deficits. Furthermore, both Nmb/Grp and Nmbr/Grpr DKO mice responded normally to a wide array of noxious stimuli. Ablation of NMBR neurons partially attenuated peripherally induced itch without compromising nociceptive processing. Importantly, electrophysiological studies suggested that GRPR neurons receive glutamatergic input from NMBR neurons. Thus, we propose that NMB and GRP may transmit discrete itch information and NMBR neurons are an integral part of neural circuits for itch in the spinal cord.
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http://dx.doi.org/10.1038/s41598-017-15756-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684337PMC
November 2017

Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil.

Antonie Van Leeuwenhoek 2017 Mar 8;110(3):297-304. Epub 2016 Nov 8.

School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.

An actinomycete strain, designated strain LUSFXJ, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA-DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642 and Streptomyces lanatus CGMCC 4.137, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJ is 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJ are MK-9(H), MK-9(H), MK-9(H) and MK-8(H). The polar lipid profile of strain LUSFXJ was found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C, anteiso-C, iso-C, C and Summed feature 3. Strain LUSFXJ was found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJ represents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJ (=GDMCC 4.133 = KCTC 39829).
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http://dx.doi.org/10.1007/s10482-016-0797-zDOI Listing
March 2017

Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations.

Sci Signal 2016 07 19;9(437):ra71. Epub 2016 Jul 19.

Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO 63110, USA. Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA. Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA. Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.

The transient receptor potential channels (TRPs) respond to chemical irritants and temperature. TRPV1 responds to the itch-inducing endogenous signal histamine, and TRPA1 responds to the itch-inducing chemical chloroquine. We showed that, in sensory neurons, TRPV4 is important for both chloroquine- and histamine-induced itch and that TRPV1 has a role in chloroquine-induced itch. Chloroquine-induced scratching was reduced in mice in which TRPV1 was knocked down or pharmacologically inhibited. Both TRPV4 and TRPV1 were present in some sensory neurons. Pharmacological blockade of either TRPV4 or TRPV1 significantly attenuated the Ca(2+) response of sensory neurons exposed to histamine or chloroquine. Knockout of Trpv1 impaired Ca(2+) responses and reduced scratching behavior evoked by a TRPV4 agonist, whereas knockout of Trpv4 did not alter TRPV1-mediated capsaicin responses. Electrophysiological analysis of human embryonic kidney (HEK) 293 cells coexpressing TRPV4 and TRPV1 revealed that the presence of both channels enhanced the activation kinetics of TRPV4 but not of TRPV1. Biochemical and biophysical studies suggested a close proximity between TRPV4 and TRPV1 in dorsal root ganglion neurons and in cultured cells. Thus, our studies identified TRPV4 as a channel that contributes to both histamine- and chloroquine-induced itch and indicated that the function of TRPV4 in itch signaling involves TRPV1-mediated facilitation. TRP facilitation through the formation of heteromeric complexes could be a prevalent mechanism by which the vast array of somatosensory information is encoded in sensory neurons.
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http://dx.doi.org/10.1126/scisignal.aaf1047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310287PMC
July 2016

Descending control of itch transmission by the serotonergic system via 5-HT1A-facilitated GRP-GRPR signaling.

Neuron 2014 Nov 30;84(4):821-34. Epub 2014 Oct 30.

Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address:

Unlabelled: Central serotonin (5-hydroxytryptophan, 5-HT) modulates somatosensory transduction, but how it achieves sensory modality-specific modulation remains unclear. Here we report that enhancing serotonergic tone via administration of 5-HT potentiates itch sensation, whereas mice lacking 5-HT or serotonergic neurons in the brainstem exhibit markedly reduced scratching behavior. Through pharmacological and behavioral screening, we identified 5-HT1A as a key receptor in facilitating gastrin-releasing peptide (GRP)-dependent scratching behavior. Coactivation of 5-HT1A and GRP receptors (GRPR) greatly potentiates subthreshold, GRP-induced Ca(2+) transients, and action potential firing of GRPR(+) neurons. Immunostaining, biochemical, and biophysical studies suggest that 5-HT1A and GRPR may function as receptor heteromeric complexes. Furthermore, 5-HT1A blockade significantly attenuates, whereas its activation contributes to, long-lasting itch transmission. Thus, our studies demonstrate that the descending 5-HT system facilitates GRP-GRPR signaling via 5-HT1A to augment itch-specific outputs, and a disruption of crosstalk between 5-HT1A and GRPR may be a useful antipruritic strategy.

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http://dx.doi.org/10.1016/j.neuron.2014.10.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4254557PMC
November 2014

RhoE is associated with relapse and prognosis of patients with colorectal cancer.

Ann Surg Oncol 2013 Jan 1;20(1):175-82. Epub 2012 Sep 1.

State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Background: RhoE, an atypical Rho protein, is differently deregulated in some solid tumors, and there are conflicting data describing the role of RhoE in tumor cell migration and invasion. This study aimed to investigate RhoE expression in human colorectal cancer and its relationship with clinicopathological features and prognosis.

Methods: Colorectal cancer and adjacent normal tissues from 202 patients were examined by immunohistochemistry. Staining evaluation results were analyzed statistically in relation to various clinicopathological parameters, disease-free survival, and overall survival. RhoE expression was also investigated by immunohistochemistry in 80 node metastases and the corresponding primary lesions, and by Western blot test in six cancer and adjacent normal tissues. The relationship between RhoE and invasion was examined by transwell assay and Western blot test.

Results: The positive rate for RhoE in colorectal cancer was significantly higher than that of normal colorectal tissues. In colorectal cancer, RhoE expression was significantly correlated with depth of invasion, lymph node metastasis, and distant metastasis. Consistently, overexpression of RhoE in SW620 cells up-regulated vimentin, down-regulated E-cadherin, increased the expression of matrix metalloproteinase (MMP) 9, and enhanced cell invasion in vitro; in contrast, silencing of RhoE by a specific siRNA caused opposite effects. Most importantly, disease-free and overall survivals were significantly poorer for patients with RhoE-positive tumors than for those with RhoE-negative tumors.

Conclusions: These findings emphasize the positive role of RhoE in invasion and metastasis in human colorectal cancer. It could also serve as an independent prognostic marker in addition to the tumor, node, metastasis staging system.
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http://dx.doi.org/10.1245/s10434-012-2472-6DOI Listing
January 2013

The TNF-α/ROS/HIF-1-induced upregulation of FoxMI expression promotes HCC proliferation and resistance to apoptosis.

Carcinogenesis 2012 Nov 25;33(11):2250-9. Epub 2012 Jul 25.

State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University Xi'an 710032, Shaanxi Province, People's Republic of China.

The proliferation-specific transcription factor Forkhead box M1 (FoxM1) acts as a master regulator of cancer cell growth and survival and plays an important role in the development of hepatocellular carcinoma. However, the molecular mechanisms that regulate FoxM1 expression remain largely unknown. In the current study, we demonstrated that tumor necrosis factor (TNF)-αα induced FoxM1 expression and transactivated its promoter activity in hepatoma cells. Serial 5" deletion and site-directed mutagenesis revealed that the induction of FoxM1 expression by TNF-α was dependent upon the hypoxia-inducible factor 1 (HIF1)-1 and HIF1-3/4 binding sites within the FoxM1 promoter. Furthermore, at the transcriptional level, the stabilization of HIF-1α via reactive oxygen species generation led to the binding of HIF-1α to the FoxM1 promoter and resulted in increased FoxM1 expression. The inhibition of both HIF-1α expression and reactive oxygen species generation significantly decreased TNF-α-induced FoxM1 overexpression. Consequently, the upregulation of FoxM1 promoted the proliferation of hepatoma cells and enhanced their resistance to TNF-α-induced apoptosis. Consistently, there was a positive correlation between HIF-1α and FoxM1 expression in 406 human hepatocellular carcinoma tissues, and the combination of these two parameters was a powerful predictor of poor prognosis in hepatocellular carcinoma patients after curative resection. Here, we report a new molecular mechanism by which FoxM1 expression is regulated by the TNF-α/reactive oxygen species/HIF-1 pathway, and this mechanism results in the proliferation of hepatoma cells and their resistance to apoptosis.
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http://dx.doi.org/10.1093/carcin/bgs249DOI Listing
November 2012

Distinguishing pancreatic cancer from chronic pancreatitis and healthy individuals by (1)H nuclear magnetic resonance-based metabonomic profiles.

Clin Biochem 2012 Sep 19;45(13-14):1064-9. Epub 2012 May 19.

State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.

Objectives: To develop a noninvasive and accessible diagnostic method for pancreatic cancer (PC).

Design And Methods: We presented a metabolomic method, pattern recognition techniques applied to (1)H nuclear magnetic resonance ((1)H NMR) spectra, to investigate the plasma metabolites obtained from 19 patients with PC, 20 patients with chronic pancreatitis (CP) and 20 healthy individuals.

Results: Metabolic changes associated with PC included abnormal amino acid and lipid metabolism, and possible multiple metabolic syndrome. PC elevated plasma levels of N-acetyl glycoprotein (NAG), dimethylamine (DMA), very low density lipoprotein (VLDL), and acetone, and reduced levels of 3-hydroxybutyrate, lactate, high density lipoprotein (HDL), low density lipoprotein (LDL), citrate, alanine, glutamate, glutamine, histidine, isoleucine, lysine, and valine. These metabolites could be a biomarker group for PC that distinguishes between PC and CP patients and healthy individuals.

Conclusions: NMR-based metabonomic strategy appears as a promising approach for distinguishing pancreatic cancer and identifying new strategies for prevention or therapy in the clinical practice.
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http://dx.doi.org/10.1016/j.clinbiochem.2012.05.012DOI Listing
September 2012

CacyBP/SIP protein promotes proliferation and G1/S transition of human pancreatic cancer cells.

Mol Carcinog 2011 Oct 25;50(10):804-10. Epub 2011 Jan 25.

State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi, China.

Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of the ubiquitin-mediated proteolysis, could participate in beta-catenin degradation, which was found to be related to the malignant phenotypes of pancreatic cancer previously. However, the role of CacyBP/SIP itself in pancreatic cancer has not been investigated. In the present study, CacyBP/SIP expression was assayed and manipulated to reveal the potential mechanism in pancreatic cancer carcinogenesis. Here, we show that CacyBP/SIP is over-expressed in pancreatic cancer cells. Down-regulation of CacyBP/SIP by small interference RNA (siRNA) severely suppresses the proliferation and tumorigenesis in pancreatic cancer. G1/S transition arrest induced by inhibition of CacyBP/SIP is at least partly mediated by down-regulation of Cyclin E and CDK2 as well as up-regulation of p27 and Rb. Collectively, CacyBP/SIP as an enhancer of pancreatic cancer malignance might develop into another possible therapeutic target.
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http://dx.doi.org/10.1002/mc.20737DOI Listing
October 2011

Adenoviral-mediated RNA interference targeting URG11 inhibits growth of human hepatocellular carcinoma.

Int J Cancer 2011 Jun 8;128(12):2980-93. Epub 2010 Oct 8.

State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.

Hepatocellular carcinoma (HCC) is the second most common malignancy in Asia, with a 5-year survival rate of less than 5% due to high recurrence after surgery and resistance to chemotherapy. A variety of therapeutic interventions to treat HCC, particularly gene therapy, have recently been investigated in tumor model systems to provide a more complete understanding of hepatocarcinogenesis and effectively design therapeutic strategies to treat this disease. In our study, we constructed an adenoviral vector expressing small interfering RNA (siRNA) targeting a newly discovered gene named upregulated gene 11 (URG11). We introduced this vector into HCC cells to investigate the role of URG11 in HCC carcinogenesis. We observed that upon URG11 knockdown, HCC cell proliferation was inhibited through downregulation of several G1-S phase related molecules including cyclin D1 and apoptosis was induced as a result of Bcl-2 downregulation. Besides decreased expression of cyclin D1, CDK4, pRb and Bcl-2, URG11 also suppressed several other proteins including CAPN9, which was identified by cDNA microarray and 2D gel electrophoresis. Moreover, Ad-URG11-siRNA significantly suppressed HCC tumor growth in nude mice. In conclusion, Ad-URG11-siRNA can significantly suppress HCC tumor growth in vitro and in vivo by silencing the URG11 gene, and the use of this vector for gene therapy may represent a novel strategy to treat human HCC.
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http://dx.doi.org/10.1002/ijc.25624DOI Listing
June 2011

[Effect of design and operation parameters on volatile alkylsulfides removal in subsurface constructed wetlands].

Huan Jing Ke Xue 2010 Feb;31(2):345-51

Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

A pilot-scale subsurface constructed wetland wastewater treatment system was sampled for one year to study the effects of bed aspect ratio, substrate medium size, water depth, HLR (hydraulic loading rate) and temperature (season) on removal of volatile alkylsulfides such as DMS (dimethylsulfide) and DMDS (dimethyldisulfide). The yearly experimental results demonstrated that the system showed good performance for DMS and DMDS removal in wastewater under different HLR ranging from 12 cm x d(-1) to 86 cm x d(-1). The system could remove 86% of DMS, and 95% of DMDS, respectively. ANOVA statistical analysis shows that HLR and temperature (season) are major factors controlling the system performance for the target analytes. According to ANOVA test, the HLR caused significant differences (p < 0.01) on the average DMS effluent concentrations, and temperature (season) caused significant differences (p < 0.01) on the average DMS and DMDS effluent concentrations. However, bed aspect ratio, substrate medium size and water depth did not cause significant differences (p > 0.05) on the average DMS and DMDS effluent concentrations. A survey of dissolved oxygen and ORP indicates that the constructed wetlands system showed strong reduced condition. On the basis of investigations of electron acceptors (such as SO4(2-), NO3- and NO2-) and dissolved organic pollutants (such as TOC and acetic acid) concentrations along with the length of constructed wetlands, it can be concluded that sulfate reduction and methanogenisis were estimated to be significant for DMS and DMDS removal in constructed wetland beds.
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February 2010

Effects of mu and kappa opioid receptor agonists and antagonists on contraction of isolated colon strips of rats with cathartic colon.

World J Gastroenterol 2004 Jun;10(11):1672-4

Department of General Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.

Aim: To study the effects of mu and kappa opioid receptor agonists and antagonists on the isolated colon strips of rats with cathartic colon.

Methods: Cathartic colon model was established by feeding rats with contact laxatives, and effects of mu and kappa opioid receptor agonists and antagonists on electricity-stimulated contraction of isolated colon strips of rats with cathartic colon were observed.

Results: Compared with control group, exogenous mu and kappa agonists inhibited significantly electricity-stimulated contraction of strips of cathartic colon (8.50+/-0.89 mm, 6.24+/-0.91 mm, 3.35+/-0.6 mm vs 11.40+/-0.21 mm P<0.01; 8.98+/-0.69 mm, 6.89+/-0.71 mm, 4.43+/-0.99 mm vs 11.40+/-0.21 mm, P<0.01). In contrast, the exogenous mu antagonist significantly enhanced electricity-stimulated contraction of isolated colon strips (13.18+/-0.93 mm, 15.87+/-0.98 mm, 19.46+/-1.79 mm vs 11.40+/-0.21 mm, P<0.01), but kappa antagonist had no effect on the isolated colon strips of rats with cathartic colon.

Conclusion: Mu and kappa opioid receptors are involved in the regulation of colon motility of rats with cathartic colon.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572778PMC
http://dx.doi.org/10.3748/wjg.v10.i11.1672DOI Listing
June 2004