Publications by authors named "Ping Lu"

965 Publications

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen.

Animals (Basel) 2021 May 22;11(6). Epub 2021 May 22.

Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels ( < 0.05), while reducing the ROS and MDA levels ( < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.
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http://dx.doi.org/10.3390/ani11061506DOI Listing
May 2021

Ceramide Synthase 6 Maximizes p53 Function to Prevent Progeny Formation from Polyploid Giant Cancer Cells.

Cancers (Basel) 2021 May 5;13(9). Epub 2021 May 5.

Department of Microbiology & Immunology, Medical University of South Carolina, Charleston, SC 29425, USA.

Polyploid giant cancer cells (PGCC) constitute a transiently senescent subpopulation of cancer cells that arises in response to stress. PGCC are capable of generating progeny via a primitive, cleavage-like cell division that is dependent on the sphingolipid enzyme acid ceramidase (ASAH1). The goal of this study was to understand differences in sphingolipid metabolism between non-polyploid and polyploid cancer cells to gain an understanding of the ASAH1-dependence in the PGCC population. Steady-state and flux analysis of sphingolipids did not support our initial hypothesis that the ASAH1 product sphingosine is rapidly converted into the pro-survival lipid sphingosine-1-phosphate. Instead, our results suggest that ASAH1 activity is important for preventing the accumulation of long chain ceramides such as C-ceramide. We therefore determined how modulation of C-ceramide, either through CerS6 or p53, a known PGCC suppressor and enhancer of CerS6-derived C-ceramide, affected PGCC progeny formation. Co-expression of the CerS6 and p53 abrogated the ability of PGCC to form offspring, suggesting that the two genes form a positive feedback loop. CerS6 enhanced the effect of p53 by significantly increasing protein half-life. Our results support the idea that sphingolipid metabolism is of functional importance in PGCC and that targeting this signaling pathway has potential for clinical intervention.
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http://dx.doi.org/10.3390/cancers13092212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125704PMC
May 2021

Visible-Light-Induced Photocatalyst-Free Aerobic Hydroxyazidations of Indoles: A Highly Regioselective and Stereoselective Synthesis of -2-Azidoindolin-3-ols.

J Org Chem 2021 Jun 1. Epub 2021 Jun 1.

Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.

A visible-light-promoted aerobic hydroxyazidation of indole derivatives with TMSN is described. The reaction proceeded under photocatalyst-free conditions to furnish -2-azidoindolin-3-ols with high regioselectivity and stereoselectivity. The protocol is operationally simple, and the starting materials (e.g., 1-(pyrimidin-2-yl) indoles, azidotrimethylsilane, and air) are readily available. The proposed mechanism in which substrates act as photocatalysts upon excitation using blue light-emitting diodes (LEDs) was supported by experimental studies.
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http://dx.doi.org/10.1021/acs.joc.1c00186DOI Listing
June 2021

Photocatalytic Approach for Construction of 5,6-Dihydroimidazo[2,1-]isoquinolines and Their Luminescent Properties.

J Org Chem 2021 Jun 1. Epub 2021 Jun 1.

Department of Chemistry, Zhejiang University, Hangzhou, P. R. China.

A visible-light-driven photoredox reaction of tetrahydroisoquinoline with 2-azirines is described. 4,7-Bis(4-methoxyphenyl)benzo[][1,2,5]thiadiazole, a benzothiadiazole (BTD) derived fluorophore, is used as an organic photoredox catalyst, and the reaction offers an efficient access to 5,6-dihydroimidazo[2,1-]isoquinolines with a broad range of functional groups. The resulting 5,6-dihydroimidazo[2,1-]isoquinolines present strong photoluminecence in solutions and powders and could be applied in the fabrication of blue OLED devices.
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http://dx.doi.org/10.1021/acs.joc.1c00590DOI Listing
June 2021

Correction: Oct-4 and Nanog promote the epithelial-mesenchymal transition of breast cancer stem cells and are associated with poor prognosis in breast cancer patients.

Oncotarget 2021 May 11;12(10):1024-1025. Epub 2021 May 11.

Breast disease and Reconstruction center, Breast cancer key lab of Dalian, the Second Hospital of Dalian Medical University, Dalian, 116023, China.

[This corrects the article DOI: 10.18632/oncotarget.2506.].
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http://dx.doi.org/10.18632/oncotarget.27791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121612PMC
May 2021

Efficient Red Electroluminescence From Phenanthro[9,10-d]imidazole-Naphtho[2,3-c][1,2,5]thiadiazole Donor-Acceptor Derivatives.

Chem Asian J 2021 May 18. Epub 2021 May 18.

State Key Lab of Supramolecular Structure and Materials, Department of Chemistry, Jilin University, 2699 Qianjin Avenue, 130012, Changchun, P. R. China.

Red emission is one of the three primary colors and is indispensable for full color displays. Fluorescent materials that can generate efficient red electroluminescence (EL) are limited and need to be developed. In this work, we report efficient red emitters based on phenanthro[9,10-d]imidazole-naphtho[2,3-c][1,2,5]thiadiazole donor-acceptor derivatives. The molecules, abbreviated as PINzP and PINzPCN, exhibited high photoluminescence quantum yield (PLQY) up to unity in doped films. They can also reach a relatively high PLQY of ∼30% in neat films. PINzP and PINzPCN were capable of generating efficient red EL in doped devices with a maximum external quantum efficiency (EQE) of 6.96% and 5.92%, respectively.
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http://dx.doi.org/10.1002/asia.202100391DOI Listing
May 2021

Influences of submerged plant collapse on diet composition, breadth, and overlap among four crane species at Poyang Lake, China.

Front Zool 2021 May 17;18(1):24. Epub 2021 May 17.

The Institute of Biology and Resources, Jiangxi Academy of Sciences, Nanchang, 330096, China.

Background: Interannual variation in resource abundance has become more unpredictable, and food shortages have increasingly occurred in the recent decades. However, compared to seasonal fluctuations in resource abundance, the influences of interannual variation in resource abundance on the dietary niches of consumers remain poorly understood. Poyang Lake, China, is a very important wintering ground for the globally endangered Siberian Crane (Leucogeranus leucogeranus), White-naped Crane (Grus vipio), and Hooded Crane (G. monacha), as well as the non-endangered Eurasian Crane (G. grus). Tubers of Vallisneria spp., the dominant submerged macrophytes at Poyang Lake, is an important food for cranes. Nevertheless, submerged macrophytes have experienced serious degradation recently. In this study, we used metabarcoding technology to explore the consequences of Vallisneria tuber collapse on the diet compositions, breadths, and overlaps of the four crane species based on fecal samples collected in winter 2017 (a year with tuber collapse) and winter 2018 (a year with high tuber abundance).

Results: Compared to previous studies, our study elucidates crane diets in an unprecedented level of detail. Vallisneria tubers was confirmed as an important food source of cranes. Surprisingly, the grassland plant Polygonum criopolitanum was also found to be an important food source in the feces of cranes. Agricultural fields were important foraging sites for Siberian Cranes, White-naped Cranes, and Hooded Cranes, providing foods that allowed them to survive in winters with natural food shortages. However, the three crane species preferred natural wetlands to agricultural fields when the abundance of natural foods was high. The abundance of Vallisneria tubers, and probably P. criopolitanum, greatly influenced the dietary compositions, breadths and overlap of cranes. During periods of preferred resource shortage, White-naped Cranes and Hooded Cranes widened their dietary niches, while Siberian Cranes maintained a stable niche width. The dietary niche overlap among crane species increased substantially under conditions of plentiful preferred food resources.

Conclusions: Our study emphasizes the superior quality of natural wetlands compared to agricultural fields as foraging habitats for cranes. To provide safer and better foraging areas for cranes, it is urgent to restore the submerged plants at Poyang Lake. While high dietary niche overlap is often interpreted as intense interspecific competition, our study highlights the importance of taking food abundance into account.
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http://dx.doi.org/10.1186/s12983-021-00411-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130136PMC
May 2021

Bulked segregant CGT-Seq-facilitated map-based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides).

Plant Biotechnol J 2021 May 5. Epub 2021 May 5.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.

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http://dx.doi.org/10.1111/pbi.13609DOI Listing
May 2021

Complete chloroplast genome sequencing of Job's tears ( L.): genome structure, comparative analysis, and phylogenetic relationships.

Mitochondrial DNA B Resour 2021 Apr 15;6(4):1399-1405. Epub 2021 Apr 15.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Job's tears, also known as adlay, is a valuable plant that has commonly been used in traditional Chinese medicine, as well as an edible food. Due to the lack of knowledge of its genetics and gaps in its evolutionary analysis, breeding of adlay has been hindered. Here, we report five complete chloroplast genomes of various species and varieties in the genus by Illumina sequencing, while their genome structure, comparative analysis, and phylogenetic relationships were conducted. Genome sizes ranged from 140,860 to 140,864 bp in length, GC contents were 38.43%, and genome architecture was of a typical quadripartite structure. We annotated 82~83 protein-coding genes and 46~47 non-coding RNA genes in each genome and they functionally associated with self-replication, photosynthesis, cytochrome synthesis and other unknown functions. Three codons that encoded tryptophan, arginine and leucine were used frequently at rates of 41.42, 37.98, and 32.28% respectively. The preferred codons consistently ended with A or T. A total of 146 simple sequence repeats (SSR), 9 insertions and deletions (InDels) and 143 single nucleotide polymorphisms (SNPs) were observed among genomes. The InDel and SNP variations were mostly distributed in intergenic regions. It confirmed that , , , and were closely genera and the genetic distance of to was closer t These results give us more insight into the evolution of in a wide range of evolutionary studies.
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http://dx.doi.org/10.1080/23802359.2021.1911704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057079PMC
April 2021

Relationship Between Glycosylated Hemoglobin and Short-Term Mortality of Spontaneous Intracerebral Hemorrhage.

Front Neurol 2021 16;12:648907. Epub 2021 Apr 16.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

The relationship between glycosylated hemoglobin (HbA1c) and prognosis of spontaneous intracerebral hemorrhage (SICH) patients has not been fully elucidated. This study aimed to reveal the relationship between HbA1c levels and short-term mortality after patient admission with SICH. It was a large-scale, multicenter, cross-sectional study. From August 1, 2015, to July 31, 2019, a total of 41910 SICH patients were included in the study from the Chinese Stroke Center Alliance (CSCA) program. Finally, we comprehensively analyzed the data from 21,116 patients with SICH. HbA1c was categorized into four groups by quartile. Univariate and multivariate logistic regression analyses were used to assess the association between HbA1c levels and short-term mortality in SICH patients. The average age of the 21,116 patients was 62.8 ± 13.2 years; 13,052 (61.8%) of them were male, and 507 (2.4%) of them died. Compared to the higher three quartiles of HbA1c, the lowest quartile (≤5.10%) had higher short-term mortality. In subgroup analysis with or without diabetes mellitus (DM) patients, the mortality of the Q3 group at 5.60-6.10% was significantly lower than that of the Q1 group at ≤5.10%. After adjustment for potential influencing factors, the ROC curve of HbA1c can better predict the short-term mortality of patients with SICH (AUC = 0.6286 < 0.001). Therefore, we concluded that low or extremely low HbA1c levels (≤5.10%) after stroke were associated with higher short-term mortality in SICH patients, with or without DM.
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http://dx.doi.org/10.3389/fneur.2021.648907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085396PMC
April 2021

Visible-light driven rapid bacterial inactivation on red phosphorus/titanium oxide nanofiber heterostructures.

J Hazard Mater 2021 07 19;413:125462. Epub 2021 Feb 19.

Department of Blood Transfusion & Department of Nephrology & Medical Research Center & Department of Physical Medicine and Rehabilitation, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China; School of Environmental Science and Engineering, State Key Laboratory of Bio-fibers and Eco-textiles, Shandong Collaborative Innovation Center of Marine Biobased Fibers and Ecological Textiles, Qingdao University, Qingdao 266071, China; School of Basic Medicine, Medical College, Qingdao University, Qingdao 266071, China. Electronic address:

Photocatalytic water disinfection has emerged as a promising approach for water purification. However, exploring efficient and rapid visible light driven materials for photocatalytic bacterial inactivation is still a challenging problem. Herein, red phosphorus/titanium oxide ([email protected]) nanofibers were developed for effective water disinfection by a vacuum ampoule strategy. The complete E. coli and S. aureus (7-log CFU mL) could be rapidly killed within 25 min and 30 min over the optimized [email protected] heterostructure under the white LED irradiation. The efficient photocatalytic antibacterial activity should be mainly ascribed to the synergetic enhancement in light absorption by RP decoration and charge migration and separation by the interface between TiO and RP. And then more unpaired photo-carriers would be transferred to the surface to facilitate the generation of photo-holes, •O radicals, and HO species, which could destroy the bacterial cells efficiently.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125462DOI Listing
July 2021

Models Based on Dynamic Clinicopathological Indices for Predicting Prognosis During the Perioperative Period for Patients with Colorectal Cancer.

J Inflamm Res 2021 21;14:1591-1601. Epub 2021 Apr 21.

Department of Gastrointestinal Oncology Surgery, Hubei Cancer Hospital, The Seventh Clinical School Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Background: Recent studies have found that clinicopathological indices, such as inflammatory and biochemical indices, play a significant role in the prognosis of colorectal cancer (CRC) patients. However, few studies have focused on the effect of dynamic changes in these indicators. In our study, we studied the influence of dynamic changes in inflammatory and biochemical indices on patient outcomes during the perioperative period.

Methods: We enrolled 551 patients from Hubei Cancer Hospital who had undergone radical resection of CRC and collected the results of laboratory examinations performed within 1 week before surgery and at the first admission after surgery. The whole population was randomly divided into the training (386) and testing (185) cohorts. We used postoperative inflammatory and biochemical indices/preoperative inflammatory and biochemical indices (ΔX) to reflect the dynamic changes. Chi-square tests, Kaplan-Meier survival analyses, and univariate and multivariate Cox regression analyses were used to evaluate the prognosis. The prediction accuracies of models for overall survival (OS) and disease-free survival (DFS) were estimated through Harrell's concordance index (the C-index) and Brier scores. Nomograms of the prognostic models were plotted for evaluations of individualized outcomes.

Results: The median follow-up time of the 551 patients was 35.6 (range: 1.1-73.8) months. Ultimately, the prognostic models based on age, sex, TNM stage, pathological conditions, inflammatory and biochemical indices, CEA, and CA199 were found to have exceptional performance for OS and DFS. The C-index of the nomogram for OS was 0.806 (95% CI, 0.75-0.86) in the training cohort and 0.921 (95% CI, 0.87-0.96) in the testing cohort. The C-index of the nomogram for DFS was 0.781 (95% CI, 0.74-0.82) in the training cohort and 0.835 (95% CI, 0.78-0.88) in the testing cohort.

Conclusion: We successfully established a novel model based on inflammatory and biochemical indices to guide clinical decision-making for CRC.
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http://dx.doi.org/10.2147/JIR.S302435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071089PMC
April 2021

[Distribution of Antibiotic Resistance Genes and Microbial Communities in a Fishery Reclamation Mining Subsidence Area].

Huan Jing Ke Xue 2021 May;42(5):2541-2549

Low Carbon Energy Institute, China University of Ming and Technology, Xuzhou 221000, China.

The widespread use of antibiotics in the aquaculture industry has caused antibiotic resistance genes (ARGs) pollution. Metagenomics technology was used to detect and analyze the relative abundance of ARGs and microbial community structure in a fishery reclamation mining subsidence area. A total of 29 ARGs were detected, and had the highest relative abundance in all the samples, reaching 1.96×10-1.19×10. The relative abundance of sulfonamide and tetracycline ARGs in sediments was relatively high and the relative abundance of multidrug ARGs in well water was relatively high. Proteobacteria was the most dominant bacterial phylum in all the samples, and Chloroflexi and Euryarchaeota were relatively abundant in the sediments. was the most dominant bacterial genus in the sediments, and and were the dominant bacterial genera in the well water. The correlation analysis between the ARGs and microorganisms showed that the genera and ARGs were mainly correlated to a moderate degree, and multiple genera had significant positive correlations with ARGs. The distribution of ARGs was affected by the structure of the microbial community. The sediments and well water in the fishery reclamation mining subsidence area were both contaminated by ARGs, and corresponding control measures should be strengthened to protect the regional environment.
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http://dx.doi.org/10.13227/j.hjkx.202009166DOI Listing
May 2021

Direct validation of dune instability theory.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Laboratoire Matière et Système Complexes, Université de Paris, CNRS, 75205 Paris Cedex 13, France.

Modern dune fields are valuable sources of information for the large-scale analysis of terrestrial and planetary environments and atmospheres, but their study relies on understanding the small-scale dynamics that constantly generate new dunes and reshape older ones. Here, we designed a landscape-scale experiment at the edge of the Gobi desert, China, to quantify the development of incipient dunes under the natural action of winds. High-resolution topographic data documenting 42 mo of bedform dynamics are examined to provide a spectral analysis of dune pattern formation. We identified two successive phases in the process of dune growth, from the initial flat sand bed to a meter-high periodic pattern. We focus on the initial phase, when the linear regime of dune instability applies, and measure the growth rate of dunes of different wavelengths. We identify the existence of a maximum growth rate, which readily explains the mechanism by which dunes select their size, leading to the prevalence of a 15-m wavelength pattern. We quantitatively compare our experimental results with the prediction of the dune instability theory using transport and flow parameters independently measured in the field. The remarkable agreement between theory and observations demonstrates that the linear regime of dune growth is permanently expressed on low-amplitude bed topography, before larger regular patterns and slip faces eventually emerge. Our experiment underpins existing theoretical models for the early development of eolian dunes, which can now be used to provide reliable insights into atmospheric and surface processes on Earth and other planetary bodies.
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http://dx.doi.org/10.1073/pnas.2024105118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092407PMC
April 2021

The dual role of complement in cancers, from destroying tumors to promoting tumor development.

Cytokine 2021 Jul 10;143:155522. Epub 2021 Apr 10.

Department of Medical Oncology, Hubei Cancer Hospital, the Seventh Clinical School Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Colorectal Cancer Clinical Research Center of HuBei Province, Wuhan, China; Colorectal Cancer Clinical Research Center of Wuhan, Wuhan, China. Electronic address:

Complement is an important branch of innate immunity; however, its biological significance goes far beyond the scope of simple nonspecific defense and involves a variety of physiological functions, including the adaptive immune response. In this review, to unravel the complex relationship between complement and tumors, we reviewed the high diversity of complement components in cancer and the heterogeneity of their production and activation pathways. In the tumor microenvironment, complement plays a dual regulatory role in the occurrence and development of tumors, affecting the outcomes of the immune response. We explored the differential expression levels of various complement components in human cancers via the Oncomine database. The gene expression profiling interactive analysis (GEPIA) tool and Kaplan-Meier plotter (K-M plotter) confirmed the correlation between differentially expressed complement genes and tumor prognosis. The tumor immune estimation resource (TIMER) database was used to statistically analyze the effect of complement on tumor immune infiltration. Finally, with a view to the role of complement in regulating T cell metabolism, complement could be a potential target for immunotherapies. Targeting complement to regulate the antitumor immune response seems to have potential for future treatment strategies. However, there are still many complex problems, such as who will benefit from this therapy and how to select the right therapeutic target and determine the appropriate drug concentration. The solutions to these problems depend on a deeper understanding of complement generation, activation, and regulatory and control mechanisms.
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http://dx.doi.org/10.1016/j.cyto.2021.155522DOI Listing
July 2021

The anti-cancer effect of metoclopramide on triple-negative breast cancer cells.

Pharmazie 2021 Apr;76(4):172-174

Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan Province, P. R. China.

Triple-negative breast cancer (TNBC) is the most aggressive type of breast cancers. Chemotherapy is the most important therapeutic option for TNBC, and chemotherapy-induced nausea and vomiting (CINV) is inevitable. Metoclopramide is a good and cost-effective therapeutic option for chemotherapy-induced nausea and vomiting. However, it is not commonly used in breast cancer because it can increase serum prolactin levels by blocking dopamine D2 receptor. This study aimed at elucidating the effect of metoclopramide on triple-negative breast cancer, MDA-MB-231 cells were treated with various concentrations of metoclopramide, the cell proliferation was detected by MTT method, the apoptosis rate was detected by Annexin V/PI double staining method, the expression change of death-related protein was detected by Western Blot. We found that metoclopramide inhibits cell proliferation and induces cell apoptosis of MDA-MB-231 in a concentration-dependent manner, and the Bcl family was involved in this process.
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http://dx.doi.org/10.1691/ph.2021.0977DOI Listing
April 2021

Atomic Structure of Surface-Densified Phases in Ni-Rich Layered Compounds.

ACS Appl Mater Interfaces 2021 Apr 12;13(15):17478-17486. Epub 2021 Apr 12.

Materials Science and Engineering, Rutgers University, Newark, New Jersey 08854, United States.

In this work, we report the presence of surface-densified phases (β-NiO, γ-NiO, and δ-NiO) in LiNiO (LNO)- and LiNiAlO (LNA)-layered compounds by combined atomic level scanning transmission electron microscopy (STEM) and electron energy loss spectroscopy (EELS). These surface phases form upon electrochemical aging at high state of charge corresponding to a fully delithiated state. A unique feature of these phases is the periodic occupancy by Ni in the Li layer. This periodic Ni occupancy gives rise to extra diffraction reflections, which are qualitatively similar to those of the LiNiO spinel structure, but these surface phases have a lower Ni valence state and cation content than spinel. These experimental results confirm the presence of thermodynamically stable surface phases and provide new insights into the phenomena of surface phase formation in Ni-rich layered structures.
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http://dx.doi.org/10.1021/acsami.1c00143DOI Listing
April 2021

The Role of the Signaling Pathways Involved in the Effects of Hydrogen Sulfide on Endoplasmic Reticulum Stress.

Front Cell Dev Biol 2021 19;9:646723. Epub 2021 Mar 19.

Institute of Biomedical Informatics, Bioinformatics Center, School of Basic Medical Sciences, Henan University, Kaifeng, China.

Endoplasmic reticulum (ER) is a kind of organelle with multiple functions including protein synthesis, modification and folding, calcium storage, and lipid synthesis. Under stress conditions, ER homeostasis is disrupted, which is defined as ER stress (ERS). The accumulation of unfolded proteins in the ER triggers a stable signaling network named unfolded protein response (UPR). Hydrogen sulfide is an important signal molecule regulating various physiological and pathological processes. Recent studies have shown that HS plays an important role in many diseases by affecting ERS, but its mechanism, especially the signaling pathways, is not fully understood. Therefore, in this review, we summarize the recent studies about the signaling pathways involved in the effects of HS on ERS in diseases to provide theoretical reference for the related in-depth researches.
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http://dx.doi.org/10.3389/fcell.2021.646723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017186PMC
March 2021

Pipeline Monitoring Using Highly Sensitive Vibration Sensor Based on Fiber Ring Cavity Laser.

Sensors (Basel) 2021 Mar 16;21(6). Epub 2021 Mar 16.

National Energy Technology Laboratory, 3610 Collins Ferry Road, Morgantown, WV 26505, USA.

A vibration fiber sensor based on a fiber ring cavity laser and an interferometer based single-mode-multimode-single-mode (SMS) fiber structure is proposed and experimentally demonstrated. The SMS fiber sensor is positioned within the laser cavity, where the ring laser lasing wavelength can be swept to an optimized wavelength using a simple fiber loop design. To obtain a better signal-to-noise ratio, the ring laser lasing wavelength is tuned to the maximum gain region biasing point of the SMS transmission spectrum. A wide range of vibration frequencies from 10 Hz to 400 kHz are experimentally demonstrated. In addition, the proposed highly sensitive vibration sensor system was deployed in a field-test scenario for pipeline acoustic emission monitoring. An SMS fiber sensor is mounted on an 18" diameter pipeline, and vibrations were induced at different locations using a piezoelectric transducer. The proposed method was shown to be capable of real-time pipeline vibration monitoring.
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http://dx.doi.org/10.3390/s21062078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002286PMC
March 2021

Integrated multi-beam optical phased array based on a 4  ×  4 Butler matrix.

Opt Lett 2021 Apr;46(7):1566-1569

We design and demonstrate the first, to the best of our knowledge, silicon-based multi-beam optical phased array (MOPA), incorporating a 4×4 Butler matrix beamforming network. The one-dimensional end-fire array consists of 16 emitters at a uniform pitch together with their corresponding phase shifters and is shared among the beams to realize large-scale aliasing-free beam-steering at reduced complexity. Experimental results show that the device is capable of individual beam aliasing-free operation with a field of view up to 46°. The steering envelope shows a plateau where the peak intensities fluctuate within 0.5 dB. The beamforming and beam-steering performance are also evaluated for simultaneous multi-beam operation. Our work validates the feasibility of beamforming-network-based MOPAs, which are promising for applications including light detection and ranging and free-space optical communication.
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http://dx.doi.org/10.1364/OL.419828DOI Listing
April 2021

CD74 promotes perineural invasion of cancer cells and mediates neuroplasticity via the AKT/EGR-1/GDNF axis in pancreatic ductal adenocarcinoma.

Cancer Lett 2021 Jun 22;508:47-58. Epub 2021 Mar 22.

State Key Laboratory of Oncogenes and Related Genes, Department of Biliary-Pancreatic Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, PR China. Electronic address:

Perineural invasion (PNI) is a common feature of pancreatic ductal adenocarcinoma (PDAC) and is one of the important causes of local recurrence in resected pancreatic cancer, but the molecular mechanism remains largely unexplored. Here, we used immunohistochemistry staining to determine the expression of CD74. Then the in vivo PNI model, in vitro neuroplasticity assay, cell proliferation assay, wound healing and Transwell-based invasion assay were performed to examine the function of CD74 in pancreatic cancer cell lines. ChIP assay and Luciferase reporter assay were used to illustrate the mechanism underlying CD74 induced GDNF expression. We confirmed that the expression level of CD74 was an independent predictor of PNI and poor prognosis for PDAC. Moreover, we found that upregulation of CD74 on PDAC enhanced its migration and invasive capabilities and potentiated the secretion of neurotrophic factor GDNF to promote the neuroplasticity. Mechanistically, CD74 promoted GDNF production via the AKT/EGR-1/GDNF axis in PDAC. Taken together, our findings suggest a supportive role of CD74 in the PNI of PDAC, and deepen our understanding of how cancer cells promote neuroplasticity in the microenvironment of PDAC.
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http://dx.doi.org/10.1016/j.canlet.2021.03.016DOI Listing
June 2021

Identification of an intraocular microbiota.

Cell Discov 2021 Mar 9;7(1):13. Epub 2021 Mar 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, China.

The current dogma in ophthalmology and vision research presumes the intraocular environment to be sterile. However, recent evidence of intestinal bacterial translocation into the bloodstream and many other internal organs including the eyes, found in healthy and diseased animal models, suggests that the intraocular cavity may also be inhabited by a microbial community. Here, we tested intraocular samples from over 1000 human eyes. Using quantitative PCR, negative staining transmission electron microscopy, direct culture, and high-throughput sequencing technologies, we demonstrated the presence of intraocular bacteria. The possibility that the microbiome from these low-biomass communities could be a contamination from other tissues and reagents was carefully evaluated and excluded. We also provide preliminary evidence that a disease-specific microbial signature characterized the intraocular environment of patients with age-related macular degeneration and glaucoma, suggesting that either spontaneous or pathogenic bacterial translocation may be associated with these common sight-threatening conditions. Furthermore, we revealed the presence of an intraocular microbiome in normal eyes from non-human mammals and demonstrated that this varied across species (rat, rabbit, pig, and macaque) and was established after birth. These findings represent the first-ever evidence of intraocular microbiota in humans.
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http://dx.doi.org/10.1038/s41421-021-00245-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943566PMC
March 2021

A Low Albumin-to-Globulin Ratio Predicts a Poor Prognosis in Patients With Metastatic Non-small-cell Lung Cancer.

Front Med (Lausanne) 2021 1;8:621592. Epub 2021 Mar 1.

Department of Medical Oncology, Hubei Cancer Hospital, The Seventh Clinical School Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The serum albumin-to-globulin ratio (AGR) may be a useful prognostic factor for various cancers. This study aimed to evaluate the prognostic value of the AGR in patients with metastatic non-small-cell lung cancer (NSCLC). A retrospective study was conducted on patients with stage IV NSCLC diagnosed in Hubei Cancer Hospital from July 2012 to December 2013. The formula for calculating the AGR was serum albumin/total protein-serum albumin. The chi-square test or Fisher's exact test was used to analyze the classified variables. The Kaplan-Meier method was used to analyze the overall survival (OS) rate, which was plotted with the R language. The impact of the AGR on OS and progression-free survival (PFS) was analyzed by a multivariate Cox proportional hazard model. A total of 308 patients were included in the study population. The optimal cutoff values for the AGR in terms of OS and PFS were 1.12 and 1.09, respectively, as determined by X-Tile software. Kaplan-Meier curve analysis showed that the difference in survival rate between patients with different AGR levels was statistically significant ( = 0.04). The OS of patients with a high AGR (≥1.12) was longer than that of patients with a low AGR (<1.12). PFS in the high AGR group were better than those in the low AGR group (16.90 vs. 32.07months, = 0.008). The univariate and multivariate models proved that the AGR was an independent prognostic factor in metastatic NSCLC patients in terms of both OS ( = 0.009, hazard ratio [HR] = 0.55, 95% confidence interval [95% CI] = 0.35-0.86) and PFS ( = 0.004, HR = 0.55, 95% CI = 0.37-0.83). The AGR, which is measured in routine clinical practice, is an independent prognostic factor in terms of OS and PFS in metastatic NSCLC and can serve as a prognostic tool for metastatic NSCLC.
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http://dx.doi.org/10.3389/fmed.2021.621592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956965PMC
March 2021

The relationship between treatment-induced hypertension and efficacy of anlotinib in recurrent or metastatic esophageal squamous cell carcinoma.

Cancer Biol Med 2021 Mar 16. Epub 2021 Mar 16.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Objective: In this post-hoc analysis, we evaluated anlotinib treatment-induced hypertension as a potential predictive factor of efficacy in esophageal squamous cell carcinoma (ESCC) patients.

Methods: A total of 109 patients enrolled in the anlotinib group in a phase 2 trial were included. The tumor response was assessed by computed tomography at week 3, week 6, and then every 6 weeks until progressive disease was observed. The primary endpoint of the study was progression free survival (PFS). The secondary endpoints included overall survival (OS) and objective response rate (ORR).

Results: In all patients, the median PFS was 3.02 months [95% confidence interval (CI): 2.63-3.65 months] and the OS was 6.11 months (95% CI: 4.40-7.79 months). The ORR was 7.34% (95% CI: 3.22%-13.95%). A total of 59 (54%) patients were diagnosed with treatment-induced hypertension (Group A), and the remaining patients ( = 50, 46%) were in Group B. Baseline prognostic factors were similar between the 2 groups. Patients in Group A had a longer PFS and OS and higher ORR. When stratifying patients using a previously known history of hypertension, treatment-induced hypertension was a predictor only for patients without previous hypertension, who had longer PFS [hazard ratio (HR): 0.40, 95% CI: 0.24-0.68] and OS (HR: 0.37, 95% CI: 0.21-0.67).

Conclusions: We showed, for the first time, a correlation between treatment-induced hypertension and better prognoses in recurrent or metastatic ESCC patients treated with anlotinib, without a previously known history of hypertension. Treatment-induced hypertension may be a simple and low cost predictor for anlotinib antitumor efficacy in these patients, which may also reflect the intended target inhibition.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0187DOI Listing
March 2021

Dose escalation and expansion (phase Ia/Ib) study of GLS-010, a recombinant fully human antiprogrammed death-1 monoclonal antibody for advanced solid tumors or lymphoma.

Eur J Cancer 2021 May 7;148:1-13. Epub 2021 Mar 7.

The First Affiliated Hospital of Bengbu Medical School, No 287 Changhuai Road, Longzihu District, Bengbu, 233000, China.

Background: GLS-010, a novel engineered fully human immunoglobin G4 monoclonal antibody, can specially block the PD-1/PD-L1/2 axis and reactivate the antitumor immunity.

Aim: This phase Ia/Ib study was carried out to evaluate the safety, recommended phase II dose (R2PD), and primary antitumor effects of GLS-010 in patients with advanced, refractory lymphoma and solid tumors.

Methods: In phase Ia study, patients with refractory solid tumors and lymphoma enrolled and received GLS-010 at a dose of 1, 4, or 10 mg/kg Q2W; 240 mg Q3W or Q2W. The primary objective was to assess the dose-limiting toxicity (DLT). In phase Ib study, doses were expanded in 9 specific tumors to ensure the R2PD and explore the efficacy. Tumor mutation burden level and PD-L1 expression were also assessed with whole-exome sequencing and immunohistochemistry (SP263), respectively.

Results: Up to April 18, 2020, a total of 289 patients (n = 24, phase Ia; n = 265, phase Ib) were enrolled. DLT was not observed in phase Ia part. The T, CL, and V were similar among all dose groups and different tumors. The most common treatment-emergent adverse events (TEAEs) were anemia, leukopenia, elevated alanine aminotransaminase/asparate aminotransferase (ALT/AST), and elevated bilirubin. And hypothyroidism was the most common immune-related adverse event (irAE). The incidence of grade ≥3 TEAE was 39.8%, while grade ≥3 irAE was only 4.5%. Based on safety studies, pharmacokinetics/pharmacodynamics, and preclinical data, 240-mg Q2W was recommended as the expansion dose. The overall objective response rate was 23.6%, with 10 patients achieving complete response. Patients with a high PD-L1 expression level (31.3% Versus. 13.7%, p = 0.012) or t-issue tumor mutation burden level (31.3% Versus. 5.6%, p = 0.009) showed a significantly better response.

Conclusion: GLS-010 showed acceptable safety profile and favorable clinical response. The dose of 240 mg Q2W was an optimal recommended dose as monotherapy.
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http://dx.doi.org/10.1016/j.ejca.2021.01.020DOI Listing
May 2021

Ultrafast Laser Processing of Optical Fibers for Sensing Applications.

Sensors (Basel) 2021 Feb 19;21(4). Epub 2021 Feb 19.

National Research Council Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.

A review of recent progress in the use of infrared femtosecond lasers to fabricate optical fiber sensors that incorporate fiber Bragg gratings (FBG) and random fiber gratings (RFG) is presented. The important advancements in femtosecond laser writing based on the phase mask technique now allow through-the-coating (TTC) fabrication of Bragg gratings in ultra-thin fiber filaments, tilted fiber Bragg gratings, and 1000 °C-resistant fiber Bragg gratings with very strong cladding modes. As an example, through-the-coating femtosecond laser writing is used to manufacture distributed fiber Bragg grating sensor arrays for oil pipeline leak detection. The plane-by-plane femtosecond laser writing technique used for the inscription of random fiber gratings is also reviewed and novel applications of the resultant devices in distributed temperature sensing, fiber lasers and fiber laser sensors are discussed.
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http://dx.doi.org/10.3390/s21041447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922569PMC
February 2021

Silicate-Enhanced Heterogeneous Flow-Through Electro-Fenton System Using Iron Oxides under Nanoconfinement.

Environ Sci Technol 2021 03 24;55(6):4045-4053. Epub 2021 Feb 24.

The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Herein, a silicate-enhanced flow-through electro-Fenton system with a nanoconfined catalyst was rationally designed and demonstrated for the highly efficient, rapid, and selective degradation of antibiotic tetracycline. The key active component of this system is the FeO nanoparticle filled carbon nanotube (FeO-in-CNT) filter. Under an electric field, this composite filter enabled HO generation, which was converted to reactive oxygen species accompanied by the redox cycling of Fe/Fe. The presence of the silicate electrolyte significantly boosted the HO yield by preventing the O-O bond dissociation of the adsorbed OOH*. Compared with the surface coated FeO on the CNT (FeO-out-CNT) filter, the FeO-in-CNT filter demonstrated 1.65 times higher value toward the degradation of the antibiotic tetracycline. Electron paramagnetic resonance and radical quenching tests synergistically verified that the dominant radical species was the O or HO· in the confined FeO-in-CNT or unconfined FeO-out-CNT system, respectively. The flow-through configuration offered improved tetracycline degradation kinetics, which was 5.1 times higher (at flow rate of 1.5 mL min) than that of a conventional batch reactor. Liquid chromatography-mass spectrometry measurements and theoretical calculations suggested reduced toxicity of fragments of tetracycline formed. This study provides a novel strategy by integrating state-of-the-art material science, Fenton chemistry, and microfiltration technology for environmental remediation.
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http://dx.doi.org/10.1021/acs.est.1c00349DOI Listing
March 2021

DOCK4 Is a Platinum-Chemosensitive and Prognostic-Related Biomarker in Ovarian Cancer.

PPAR Res 2021 3;2021:6629842. Epub 2021 Feb 3.

NHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Public Health School, Fudan University, Shanghai, China.

Ovarian carcinoma (OV) is a lethal gynecological malignancy. Most OV patients develop resistance to platinum-based chemotherapy and recurrence. Peroxisome proliferator-activated receptors (PPARs) are the ligand activating transcription factor of the nuclear receptor superfamily. PPARs as important transcriptional regulators regulate important physiological processes such as lipid metabolism, inflammation, and wound healing. Several reports point out that PPARs can also have an effect on the sensitivity of tumor cells to platinum-based chemotherapy drugs. However, the role of PPAR-target related genes (PPAR-TRGs) in chemotherapeutic resistance of OV remains unclear. The present study is aimed at optimizing candidate genes by integrating platinum-chemotherapy expression data and PPAR family genes with their targets. The gene expression profiles were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database. A total of 4 genes (, , , and ) were the candidate differentially expressed genes (DEGs) of PPAR-TRGs with platinum chemosensitivity. After conducting numerous survival analyses using different cohorts, we found that only the upexpression of has important significance with the poor prognosis of OV patients. Meanwhile, is detected in plasma and enriched in neutrophil and monocyte cells of the blood. We further found that there were significant correlations between expression and the levels of CD4+ T cell infiltration, dendritic cell infiltration, and neutrophil infiltration in OV. In addition, we verified the expression level of in OV cell lines treated with platinum drugs and found that is potentially responsive to platinum drugs. In conclusion, is potentially associated with immune cell infiltration and represents a valuable prognostic biomarker in ovarian cancer patients.
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http://dx.doi.org/10.1155/2021/6629842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878079PMC
February 2021

High fat suppresses SOD1 activity by reducing copper chaperone for SOD1 associated with neurodegeneration and memory decline.

Life Sci 2021 May 16;272:119243. Epub 2021 Feb 16.

Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou 510260, China. Electronic address:

High fat consumption leads to reactive oxygen species (ROS) which is associated with age-progressive neurological disorders. Cu/Zn superoxide dismutase (SOD1) is a critical enzyme against ROS. However, the relationship between SOD1 and the high-fat-induced ROS and neurodegeneration is poorly known. Here we showed that, upon treatment with a saturated fatty acid palmitic acid (PA), the SOD1 activity was decreased in mouse neuronal HT-22 cell line accompanied by elevation of ROS, but not in mouse microglial BV-2 cell line. We further showed that PA decreased the levels of copper chaperone for SOD1 (CCS) in HT-22 cells, which promoted the nuclear import of SOD1 and decreased its activity. We demonstrated that the reduction of CCS is involved in the PA-induced decrease of SOD1 activity and elevation of ROS. In addition, compared with the adult mice fed with a standard diet, the high-fat-diet adult mice presented an increase of plasma free fatty acids, reduction of hippocampal SOD1 activity and CCS, mitochondrial degeneration and long-term memory decline. Taken together, our findings suggest that the high-fat-induced lower CCS level is essential for SOD1 suppression which may be associated with neurodegeneration and cognitive decline.
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http://dx.doi.org/10.1016/j.lfs.2021.119243DOI Listing
May 2021

LncRNA SNHG12 downregulates RAGE to attenuate hypoxia-reoxygenation-induced apoptosis in H9c2 cells.

Biosci Biotechnol Biochem 2021 Mar;85(4):866-873

The Ganzhou Hospital Affiliated to Nanchang University (The Ganzhou People's Hospital), Ganzhou, China.

Ischemia-reperfusion (I/R) injury causes cardiac dysfunction through several mechanisms including the irregular expression of some long noncoding RNA. However, the role of SNHG12 in myocardial I/R injury remains unclear. Here, we found the increase of the SNHG12 level in hypoxia-reoxygenation (H/R)-injured-H9c2 cells. SNHG12 silencing enhanced the apoptosis of H/R-injured H9c2 cells, while SNHG12 overexpression relieved the cardiomyocyte apoptosis induced by H/R stimulation. Additionally, the suppression of SNHG12 significantly boosted the H/R-induced expression and the production of TNF-α, IL-6, and IL-1β, as well as the activation of NF-κB, which were fully reversed after overexpression of SNHG12. Mechanistically, SNHG12 adversely regulated the production of receptor for advanced glycation end products (RAGE) in H/R-stimulated H9c2 cells. Antibody blocking of RAGE alleviated the apoptosis of H/R-injured H9c2 cells. Collectively, we have determined a valuable mechanism by which the high level of SNHG12 contributes to H9c2 cells against H/R injury through the reduction of RAGE expression.
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http://dx.doi.org/10.1093/bbb/zbaa090DOI Listing
March 2021