Publications by authors named "Ping Liang"

592 Publications

Correction to: MicroRNA-351 Promotes the Proliferation and Invasion of Glioma Cells Through Downregulation of NAIF1.

J Mol Neurosci 2021 Nov 16. Epub 2021 Nov 16.

Department of Neurosurgery, Children's Hospital of Chongqing Medical University, Chongqing Key Laboratory of Pediatrics, No. 136 Zhongshan 2nd Road, Chongqing, 400014, Yuzhong, China.

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http://dx.doi.org/10.1007/s12031-021-01925-4DOI Listing
November 2021

Review of clinical tumor ablation advance in Asia.

Int J Hyperthermia 2021 ;38(1):1639-1649

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Tumor ablation has been widely applied in Asia, accounting for 44.65% of clinical studies worldwide. We reviewed 5853 clinical studies to provide insight on the advance of tumor ablation in Asia chronologically and geographically among different techniques and organs. Since 1998, tumor ablation application has dramatically evolved in Asia. All kinds of ablation techniques, including percutaneous ethanol injection (PEI), radiofrequency ablation (RFA), microwave ablation (MWA), laser ablation (LA), cryoablation (CA), high-intensity focused ultrasound (HIFU), and irreversible electroporation (IRE), have been applied, with the first application of PEI and the most popular application of RFA. Twenty-five countries and one district in Asia have applied tumor ablation in various organs, including liver, lung, uterus, thyroid, kidney, pancreas, bone, prostate, breast, adrenal gland, lymph node parathyroid, esophagus, etc. Due to the high incidence of tumors as well as advanced economy and technology, East Asia accounted for 93.87% of studies, led by China (45.00%), Japan (32.72%), South Korea (12.10%), and Taiwan (4.03%). With the enrichment of evidence from large-scale multicenter and randomized control studies, China and South Korea have issued several guidelines on tumor ablation for liver, lung, and thyroid, which provided recommendations for global standardization of tumor ablation techniques. Therefore, Asia has made active contribution to global tumor ablation therapy.KeypointsKey point 1: Asia accounted for 44.65% of clinical studies worldwide on tumor ablation.Key point 2: Twenty-five countries and one district in Asia have used tumor ablation in various organs, and East Asia accounted for 93.87% of studies, led by China (45.00%), Japan (32.72%), South Korea (12.10%), and Taiwan (4.03%).Key point 3: China and South Korea have issued several guidelines on tumor ablation for liver, lung, and thyroid, which provided recommendations for global standardization of tumor ablation techniques.
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http://dx.doi.org/10.1080/02656736.2021.1983037DOI Listing
January 2021

The association between environmental exposure to perchlorate, nitrate, and thiocyanate and all-cause and cause-specific mortality.

Environ Sci Pollut Res Int 2021 Nov 12. Epub 2021 Nov 12.

Department of Cardiology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Center for Translational Medicine, The Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The aim is to examine the environmental exposure to perchlorate, nitrate, and thiocyanate and their associations with all-cause and cause-specific mortality in a general population. A total of 17,982 participants were enrolled from the National Health and Nutrition Examination Survey and urinary perchlorate, nitrate, and thiocyanate are measured using ion chromatography coupled with electrospray tandem mass spectrometry. Multivariable linear and logistic regression models were performed to explore the associations between urinary perchlorate, nitrate, and thiocyanate with all-cause and cause-specific mortality. Restricted cubic spline models were used to explore the nonlinearity. During a 7.5-year of follow-up, 1730 deaths occurred. Kaplan-Meier analysis suggested that urinary perchlorate, thiocyanate, and nitrate were associated with all-cause mortality (all log-rank P<0.001). After adjusted for traditional risk factors, we found that urinary perchlorate was inversely associated with cancer mortality (hazard ratio [HR] 0.87; 95% confidence interval [0.76, 0.99]; P=0.038) while nitrate was positively related to cancer mortality (HR 1.19, [1.05, 1.34]; P=0.006). Besides, urinary thiocyanate exposure was U-shape associated with the risk of all-cause mortality (P for nonlinearity<0.001). Urinary perchlorate was inversely while nitrate was positively associated with the risk of cancer mortality. The thiocyanate exposure was U-shape associated with the risk of all-cause mortality.
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http://dx.doi.org/10.1007/s11356-021-17423-4DOI Listing
November 2021

Requirements for human cardiomyocytes.

Cell Prolif 2021 Oct 27:e13150. Epub 2021 Oct 27.

Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou, China.

'Requirements for human cardiomyocytes', jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research, is the first guideline for human cardiomyocytes in China. This standard specifies the technical requirements, test methods, test regulations, instructions for use, labelling requirements, packing requirements, storage requirements, transportation requirements and waste disposal requirements for human cardiomyocytes, which is designed to normalize and standardize human cardiomyocyte research and production. It was originally released by the China Society for Cell Biology on 9 January 2021. We hope that the publication of this guideline will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardization of human cardiomyocytes for applications.
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http://dx.doi.org/10.1111/cpr.13150DOI Listing
October 2021

Noninvasive assessment of kidney dysfunction in children by using blood oxygenation level-dependent MRI and intravoxel incoherent motion diffusion-weighted imaging.

Insights Imaging 2021 Oct 21;12(1):146. Epub 2021 Oct 21.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, Hubei, China.

Objectives: To explore whether multiparametric approach including blood oxygenation level-dependent MRI (BOLD-MRI) and intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) can be applied in the assessment of renal function in children with chronic kidney disease (CKD).

Materials And Methods: This prospective study included 74 children (CKD stage 1-3, 51; CKD stage 4-5, 12; healthy volunteers, 11) for renal MRI examinations including coronal T2WI, axial T1WI and T2WI, BOLD-MRI, and DWI sequences. We measured the renal cortex and medulla T2*, ADC, D, D, and f values on BOLD and DWI images. Appropriate statistical methods were applied for comparing MRI-derived parameters among the three groups and calculating the correlation coefficients between MRI-derived parameters and clinical data. Receiver operating characteristic (ROC) curves were used to assess the diagnostic performance of MRI-derived parameters.

Results: There were significant differences in cortex T2*, ADC, D, f and medulla T2*, ADC, D among the three groups. Cortex T2*, ADC, D, f and medulla T2*, ADC, D had a trend: CKD stage 4-5 < CKD stage 1-3 < healthy volunteers. Cortex and medulla T2*, ADC, D were significantly correlated with eGFR, serum creatinine (Scr), cystatin C. In addition, cortex T2* and eGFR showed the highest correlation coefficient (r = 0.824, p < 0.001). Cortex D and medulla T2* were optimal parameters for differentiating healthy volunteers and CKD stage 1-3 or CKD stage 4-5 and CKD stage 1-3, respectively.

Conclusions: BOLD-MRI and IVIM-DWI might be used as a feasible method for noninvasive assessment of renal function in children with CKD.
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http://dx.doi.org/10.1186/s13244-021-01091-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531182PMC
October 2021

Matrix solid-phase dispersion based on cucurbit[7]uril-assisted dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for the determination of benzimidazole fungicides from vegetables.

J Chromatogr A 2021 Nov 2;1658:462592. Epub 2021 Oct 2.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China; Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guiyang 550025, China. Electronic address:

A new method involving matrix solid-phase dispersion (MSPD) and dispersive liquid-liquid microextraction (DLLME) was optimized with the aid of stoichiometry and applied to the extraction, purification, and determination of benzimidazole residues in vegetables. Carbendazim, thiabendazole, and thiophanate-methyl were selectively extracted from vegetables using cucurbit[7]uril as the MSPD extractant and transferred to an aqueous solution, then further enriched using DLLME with acetonitrile and chloroform as dispersive and extraction solvents, respectively. The optimal extraction conditions of MSPD and DLLME were selected by two-level full-factorial design and central-composite design (CCD). The developed method (MSPD-DLLME-HPLC-UV) showed good linearity in the range of 0.025-5 μg/g, with R > 0.9984. Intra- and interday precisions were 5.3-10.9% and 10.6-12.4%, respectively, and the limit of detection was between 0.004 and 0.007 μg/g of fresh weight. This method was applied to the analysis of four different types of vegetables, and the recoveries ranged from 65.4% to 124.0%. The method was environmentally friendly, easy to operate, and sensitive.
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http://dx.doi.org/10.1016/j.chroma.2021.462592DOI Listing
November 2021

Development of a Toll-Like Receptor-Based Gene Signature That Can Predict Prognosis, Tumor Microenvironment, and Chemotherapy Response for Hepatocellular Carcinoma.

Front Mol Biosci 2021 21;8:729789. Epub 2021 Sep 21.

Department of Interventional Ultrasound, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.

Emerging evidence highlights the implications of the toll-like receptor (TLR) signaling pathway in the pathogenesis and therapeutic regimens of hepatocellular carcinoma (HCC). Herein, a prognostic TLR-based gene signature was conducted for HCC. HCC-specific TLRs were screened in the TCGA cohort. A LASSO model was constructed based on prognosis-related HCC-specific TLRs. The predictive efficacy, sensitivity, and independency of this signature was then evaluated and externally verified in the ICGC, GSE14520, and GSE76427 cohorts. The associations between this signature and tumor microenvironment (stromal/immune score, immune checkpoint expression, and immune cell infiltrations) and chemotherapy response were assessed in HCC specimens. The expression of TLRs in this signature was verified in HCC and normal liver tissues by Western blot. Following si-MAP2K2 transfection, colony formation and apoptosis of Huh7 and HepG2 cells were examined. Herein, we identified 60 HCC-specific TLRs. A TLR-based gene signature (MAP2K2, IRAK1, RAC1, TRAF3, MAP3K7, and SPP1) was conducted for HCC prognosis. High-risk patients exhibited undesirable outcomes. ROC curves confirmed the well prediction performance of this signature. Multivariate Cox regression analysis demonstrated that the signature was an independent prognostic indicator. Also, high-risk HCC was characterized by an increased immune score, immune checkpoint expression, and immune cell infiltration. Meanwhile, high-risk patients displayed higher sensitivity to gemcitabine and cisplatin. The dysregulation of TLRs in the signature was confirmed in HCC. MAP2K2 knockdown weakened colony formation and elevated apoptosis of Huh7 and HepG2 cells. Collectively, this TLR-based gene signature might assist clinicians to select personalized therapy programs for HCC patients.
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http://dx.doi.org/10.3389/fmolb.2021.729789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490642PMC
September 2021

Survival benefits analyses of T1a renal cell carcinoma patients treated with microwave ablation.

Eur J Radiol 2021 Nov 17;144:109951. Epub 2021 Sep 17.

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, China. Electronic address:

Purpose: The individual decision-making reference of Microwave ablation (MWA) for T1a RCC treatment is not clear, and it may not benefit all the patients equally. Therefore, we quantitatively evaluated the distinct survival benefits of patients with T1a RCC stratified by survival predictors.

Materials And Methods: A total of 237 patients with T1a RCC who underwent MWA over the last 16 years were retrospectively reviewed for survival benefit analysis. Cox proportional hazard models were used to control for the prognostic variables of OS, CSS, and PFS. Survival rates were calculated using the Kaplan-Meier method and compared by log-rank analysis. Linear extrapolation was used to compute median survival periods.

Results: The OS benefit was significantly dependent on age (HR:2.499, 95% CI: 1.245-5.016, p = 0.010) and age-adjusted Charlson comorbidity index (CCI) score (HR:3.956, 95% CI, 1.409-11.110, p = 0.009). OS in patients aged <75 years or with an age-adjusted CCI score <7 was significantly prolonged (44.68, 65.55 months) compared to OS in patients aged ≥75 years or with CCI score ≥7 (p < 0.001 for both). PFS benefit was significantly dependent on age-adjusted CCI (HR:3.325, 95% CI, 1.390-7.956, p = 0.007), patient type (HR:0.4, 95% CI, 0.214-0.745, p = 0.004), and tumour growth pattern (HR:12.562, 95% CI, 1.552-101.696, p = 0.018). PFS in incipient patients was significantly prolonged (33.75 months) compared to that in the relapsed patients (p = 0.037). Patients with an age-adjusted CCI score <7 or without tumour protruding into the renal pelvis, lived free from recurrence or metastasis (55.69 or 101.61 months) longer than that in patients with an age-adjusted CCI score ≥7 or with tumour protruding into the renal pelvis (p < 0.01 for both). None of the variables was associated with CSS benefit.

Conclusions: The OS and PFS benefits from MWA were not equal for all T1a RCC patients. Age ≥75 years and age-adjusted CCI ≥ 7 significantly shortened OS. Age-adjusted CCI ≥ 7, relapsed RCC, and RCC protruding into the renal pelvis significantly shortened the PFS period. For a better survival prognosis, appropriate patient triage is still needed.
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http://dx.doi.org/10.1016/j.ejrad.2021.109951DOI Listing
November 2021

Insight into enhanced activation of permanganate under simulated solar irradiation: Rapid formation of manganese species.

Water Res 2021 Oct 16;205:117669. Epub 2021 Sep 16.

State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

Herein, permanganate [Mn(VII)] was activated by simulated solar (SS) (SS/Mn(VII)), resulting in rapid degradation of micropollutants in several minutes, with rates of target micropollutants outnumbered those in the Mn(VII) alone and SS. To explore the mechanism in this process, 4-cholorphenol (4-CP), p-hydroxybenzoic acid (p-HBA), and enrofloxacin (ENR) were selected as model compounds. Lines of evidence indicated that reactive manganese species (RMnS) (i.e., Mn(III) and Mn(V)) rather than radicals from Mn(VII) photolysis participated in the conversion of model compounds. Interestingly, roles of RMnS differed among three model compounds, suggesting their selectivity toward micropollutants. Increasing Mn(VII) dosage proved greater micropollutant degradation, while impacts of pH on SS/Mn(VII) performance varied among model compounds. P-HBA and ENR showed the lowest degradation efficiency at alkaline, whereas 4-CP demonstrated the best performance at alkaline, indicating the reactivity of RMnS varied toward micropollutants at different pH values. The quantum yield of Mn(VII) was 8.36 ± 0.03 X 10 mol Einstein at pH 7.0. Effects of common co-existing constituents (Cl, HCO, and humic acid (HA)) on micropollutant degradation by SS/Mn(VII) were examined. Specifically, HCO positively influenced the 4-CP and p-HBA degradation, whereas ENR was not affected, likely owing to the selectivity of RMnS-HCO complexes. HA was conducive to degrade p-HBA due to the production of RMnS-HA complexes, but unfavorable for ENR and 4-CP degradation because of the competitive light absorption and Mn(VII). Furthermore, a number of degradation products of 4-CP, p-HBA, and ENR were identified and possible pathways were proposed accordingly. The effectiveness of this process for micropollutant degradation in real waters, natural sunlight, ultraviolet and visible light via cut-off filtering SS emission was confirmed. This work revealed a great potential of applying SS/Mn(VII) for the marked degradation of micropollutants and facilitated the understandings of Mn(III)/Mn(V) behaviors.
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http://dx.doi.org/10.1016/j.watres.2021.117669DOI Listing
October 2021

Microwave ablation surgical resection for treatment naïve hepatocellular carcinoma within the Milan criteria: a follow-up of at least 5 years.

Cancer Biol Med 2021 Sep 30. Epub 2021 Sep 30.

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, China.

Objective: Thermal ablation poses challenges in the surgical resection (SR) of small hepatocellular carcinoma (HCC), and its therapeutic outcomes for larger lesions remain debated.

Methods: This retrospective study evaluated 729 patients with HCC meeting the Milan criteria, who were treated with curative SR or microwave ablation (MWA) between 2008 and 2014. Overall survival (OS), cancer-specific survival (CSS), disease-free survival (DFS), and local tumor progression (LTP) were compared after propensity score matching (PSM). Co-variates associated with OS, CSS, LTP, and DFS were identified. The risk of death and tumor progression were compared.

Results: During the median follow-up of 78.6 months, 253 patients were included in each group after PSM. For tumors ≤ 3.0 cm and 3.1-4.0 cm, MWA achieved comparable results in terms of OS, CSS, DFS, and LTP. For tumors 4.1-5.0 cm, MWA had lower OS, CSS, and DFS rates (all < 0.05) than SR. Higher LTP rates were observed in the MWA group for tumors 4.1-5.0 cm, although the difference was not significant ( = 0.18). Complication rates ( = 0.41) were similar, but MWA led to less estimated blood loss ( < 0.01) and shorter postoperative hospitalization times ( < 0.01).

Conclusions: MWA achieved comparable long-term oncologic outcomes with SR for ≤ 4 cm HCC, with lower complication rates and faster recovery.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0625DOI Listing
September 2021

Characterization of the molecular mechanisms underlying azithromycin-induced cardiotoxicity using human-induced pluripotent stem cell-derived cardiomyocytes.

Clin Transl Med 2021 09;11(9):e549

Key Laboratory of combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, China.

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http://dx.doi.org/10.1002/ctm2.549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444559PMC
September 2021

Hole-filling based on content loss indexed 3D partial convolution network for freehand ultrasound reconstruction.

Comput Methods Programs Biomed 2021 Nov 16;211:106421. Epub 2021 Sep 16.

Laboratory of Beijing Engineering Research Center of Mixed Reality and Advanced Display, School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China. Electronic address:

Background And Objective: During the 3D reconstruction of ultrasound volume from 2D B-scan ultrasound images, holes are usually found in the reconstructed 3D volumes due to the fast scans. This condition will affect the positioning and judgment of the doctor to the lesion. Hence, in this study, we propose to fill the holes by using a novel content loss indexed 3D partial convolution network for 3D freehand ultrasound volume reconstruction. The network can synthesize novel ultrasound volume structures and reconstruct ultrasound volume with missing regions with variable sizes and at arbitrary locations.

Methods: First, the 3D partial convolution is introduced into the convolutional layer, which is masked and renormalized to be conditioned on only valid voxels. Then, the mask in the next layer is automatically updated as a part of the forward pass. To better preserve texture and structure details of the reconstruction results, we couple the adversarial loss of the least squares generative adversarial network (LSGAN) with the innovative content loss, which consists of the context loss, the feature-matching loss and the total variation loss. Thereafter, we introduce a novel spectral-normalized LSGAN by adding spectral normalization (SN) to the generator and discriminator of the LSGAN. The proposed method is simple in formulation, and is stable in training.

Results: Experiments on public and in-vivo ultrasound datasets and comparisons with popular algorithms demonstrate that the proposed approach can generate high-quality hole-filling results with preserved perceptual image details.

Conclusions: Considering the high quality of the hole-filling results, the proposed method can effectively fill the missing regions in the reconstructed 3D ultrasound volume from 2D ultrasound image sequences.
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http://dx.doi.org/10.1016/j.cmpb.2021.106421DOI Listing
November 2021

Consensus Guidelines for the Definition of Time-to-Event End Points in Image-guided Tumor Ablation: Results of the SIO and DATECAN Initiative.

Radiology 2021 12 28;301(3):533-540. Epub 2021 Sep 28.

From the Department of Radiology, Center of Image-guided Tumor Ablation, Amsterdam University Medical Centers, Location VUMC, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands (R.S.P., H.J.S., M.R.M.); Department of Radiology, Onze Lieve Vrouwe Gasthuis (OLVG) Hospital, Amsterdam, the Netherlands (R.S.P.); Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Mass (M.A., S.N.G.); Department of Radiology, King's College, St Thomas' Hospital, London, England (A.A.); Department of Radiology, National Cancer Center Hospital, Tokyo, Japan (Y.A.); Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Mass (R.A., D.A.G.); Department of Radiology, Institut de Cancérologie Gustave Roussy, Villejuif, France (T.d.B.); Department of Radiology, Medical University Innsbruck, Innsbruck, Austria (R.B.); Clinical and Epidemiological Research Unit, Comprehensive Cancer Centre, Institut Bergonié, Bordeaux, France (C.B.); Department of Radiology, Kantonsspital Winterthur, Winterthur, Switzerland (C.A.B.); Department of Radiology, Biomedical Engineering, and Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, Wis (C.L.B., F.T.L.); Department of Radiology, University Hospital Southampton, Southampton, England (D.J.B.); 2nd Department of Radiology, University General Hospital "ATTIKON" Medical School, National and Kapodistrian University of Athens, Athens, Greece (E.B., D.F., A.K.); Department of Radiology, Mayo Clinic, Rochester, Minn (M.R.C.); Department of Radiology, Dipartimento di Scienze Medico-Chirurgiche, IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy (G.C.); Department of Radiology, Yale University School of Medicine, New Haven, Conn (J.C., H.S.K., D.C.M.); Department of Radiology, IRCCS Ospedale San Raffaele, Milan, Italy (F.d.C.); Department of Epidemiology and Data Science, Decision Modeling Center, Amsterdam University Medical Centers, Location VUMC, Amsterdam, the Netherlands (V.M.H.C., B.I.L.W.); Department of Radiology, University of Pisa, Pisa University Hospital, Pisa, Italy (L.C.); Department of Radiology, CHUV University of Lausanne, Lausanne, Switzerland (A.D.); and Department of Radiology, Brown Medical School, Cape Cod Hospital, Hyannis, Mass (D.E.D.); Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY (J.P.E., C.T.S., S.B.S.); Department of Radiology, University Hospital of Strasbourg, Strasbourg, France (A.G.); Department of Radiology, The London Clinic, London, England (A.R.G.); Society of Interventional Oncology, Washington, DC (T.G.); Department of Radiology, University Hospital of Dijon, Dijon, France (B.G.); Department of Radiology, University Hospitals Schlewsig-Holstein, Lübeck, Germany (T.H.); Department of Radiology, Agostino Gemelli University Policlinic, Catholic University of the Sacred Heart, Rome, Italy (R.I.); Department of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea (T.W.K., M.W.L., H.R.); Department of Radiology, Universitätsklinikum Augsburg, Augsburg, Germany (T.K.); Department of Radiology, University of Washington Medical Center, Seattle, Wash (S.K.); Department of Radiology, David Geffen School of Medicine, Ronald Reagan UCLA Medical Center, Los Angeles, Calif (E.W.L., D.S.L.); Department of Radiology, Chinese PLA General Hospital, Beijing, China (P.L.); Department of Radiology, IEO Istituto Europeo di Oncologia, Gruppo Ospedaliero San Donato, Milan, Italy (G.M.); Department of Radiology, Ospedale Valduce, Como, Italy (M.F.M.); Department of Radiology, St George's University Hospitals, London, England (R.M.); Department of Radiology, Hospital of the University of Pennsylvania, Philadelphia, Pa (G. Nadolski, M.C.S.); Department of Radiology, Baptist Health of South Florida, Miami Cardiac and Vascular Institute, Miami, Fla (G. Narayanan); Department of Radiology, University of California, San Diego Health, San Diego, Calif (I.N.); Department of Radiology, Cooley Dickinson Hospital, Northampton, Mass (B.N.); Department of Radiology, European Institute of Oncology, IRCCS, Milan, Italy (F.O.); Department of Radiology, Academic Hospital Ruprecht-Karls-University Heidelberg, SLK Clinics GmbH, Heilbronn, Germany (P.L.P.); Department of Radiology, Tan Tock Seng Hospital, Singapore (U.P.); Department of Radiology, Klinikum der Universität München, Munich, Germany (J.R.); Department of Radiology, Medical College of Wisconsin, Milwaukee, Wis (W.R.); Department of Radiology, Northwestern University, Chicago, Ill (R.S.); Department of Radiology, Humanitas University, Ospedale Generale, Milan, Italy (L.A.S.); Department of Radiology, Stanford University, Stanford University Medical Center, Stanford, Calif (D.S., D.S.W.); Department of Radiology, John Radcliffe Hospital, Oxford, England (R.U.); Department of Radiology, University of Frankfurt, University Hospital Frankfurt Johann Wolfgang Goethe-University, Frankfurt, Germany (T.J.V.); and Department of Radiology and Imaging Science, National Institutes of Health, Bethesda, Md (B.J.W.); Department of Radiology, Image-guided Therapy and Interventional Oncology Unit, Hadassah Hebrew University Hospital, Jerusalem, Israel (S.N.G.); and Department of Radiology, Noordwest Ziekenhuisgroep, Alkmaar, the Netherlands (M.R.M.).

There is currently no consensus regarding preferred clinical outcome measures following image-guided tumor ablation or clear definitions of oncologic end points. This consensus document proposes standardized definitions for a broad range of oncologic outcome measures with recommendations on how to uniformly document, analyze, and report outcomes. The initiative was coordinated by the Society of Interventional Oncology in collaboration with the Definition for the Assessment of Time-to-Event End Points in Cancer Trials, or DATECAN, group. According to predefined criteria, based on experience with clinical trials, an international panel of 62 experts convened. Recommendations were developed using the validated three-step modified Delphi consensus method. Consensus was reached on when to assess outcomes per patient, per session, or per tumor; on starting and ending time and survival time definitions; and on time-to-event end points. Although no consensus was reached on the preferred classification system to report complications, quality of life, and health economics issues, the panel did agree on using the most recent version of a validated patient-reported outcome questionnaire. This article provides a framework of key opinion leader recommendations with the intent to facilitate a clear interpretation of results and standardize worldwide communication. Widespread adoption will improve reproducibility, allow for accurate comparisons, and avoid misinterpretations in the field of interventional oncology research. Published under a CC BY 4.0 license. See also the editorial by Liddell in this issue.
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http://dx.doi.org/10.1148/radiol.2021203715DOI Listing
December 2021

Clinical and Radiological Features of Urachal Carcinoma and Infection.

Front Oncol 2021 7;11:702116. Epub 2021 Sep 7.

Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Purpose: To explore the clinical and radiological differences between urachal carcinoma and urachal infection.

Methods: Clinical and imaging information for 13 cases of urachal carcinoma and 14 cases of urachal infection confirmed by pathology were retrospectively analyzed. The size, location, shape, margin, lesion composition, calcification, T1 and T2 signal intensity, peripheral lymph nodes, degree of enhancement, adjacent bladder wall, and apparent diffusion coefficient (ADC) value were examined in both groups, and distinguish features were determined. The student -test or Mann-Whitney test was used for quantitative data, and Fisher's exact test was used for qualitative data. Kappa coefficient consistency test was used to evaluate the interobserver agreement.

Results: Sex, hematuria, abdominal pain, calcification, and thickening of adjacent bladder wall can distinguish between urachal carcinoma and urachal infection ( < 0.05). There were no statistical differences in age ( = 0.076), size ( = 0.797), location ( = 0.440), shape ( = 0.449), margin ( = 0.449), lesion composition ( = 0.459), T1 signal intensity ( = 0.196), T2 signal intensity ( = 0.555), peripheral lymph nodes ( = 0.236), degree of enhancements ( = 0.184) and ADC value ( = 0.780) between two groups.

Conclusion: The following clinical and imaging features help distinguish urachal carcinoma from urachal infection: sex, hematuria, abdominal pain, calcification, and thickening of the adjacent bladder wall.
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http://dx.doi.org/10.3389/fonc.2021.702116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454411PMC
September 2021

E2A ablation enhances proportion of nodal-like cardiomyocytes in cardiac-specific differentiation of human embryonic stem cells.

EBioMedicine 2021 Sep 3;71:103575. Epub 2021 Sep 3.

Department of Physiology and Pathophysiology, Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. Electronic address:

Background: Human sinoatrial cardiomyocytes are essential building blocks for cell therapies of conduction system disorders. However, current differentiation protocols for deriving nodal cardiomyocytes from human pluripotent stem cells (hPSCs) are very inefficient.

Methods: By employing the hPSCs to cardiomyocyte (CM) in vitro differentiation system and generating E2A-knockout hESCs using CRISPR/Cas9 gene editing technology, we analyze the functions of E2A in CM differentiation.

Findings: We found that knockout of the transcription factor E2A substantially increased the proportion of nodal-like cells in hESC-derived CMs. The E2A ablated CMs displayed smaller cell size, increased beating rates, weaker contractile force, and other functional characteristics similar to sinoatrial node (SAN) cells. Transcriptomic analyses indicated that ion channel-encoding genes were up-regulated in E2A ablated CMs. E2A directly bounded to the promoters of genes key to SAN development via conserved E-box motif, and promoted their expression. Unexpect enhanced activity of NOTCH pathway after E2A ablation could also facilate to induct ventricle workingtype CMs reprogramming into SAN-like cells.

Interpretation: Our study revealed a new role for E2A during directed cardiac differentiation of hESCs and may provide new clues for enhancing induction efficiency of SAN-like cardiomyocytes from hPSCs in the future.

Funding: This work was supported by the NSFC (No.82070391, N.S.; No.81870175 and 81922006, P.L.), the National Key R&D Program of China (2018YFC2000202, N.S.; 2017YFA0103700, P.L.), the Haiju program of National Children's Medical Center EK1125180102, and Innovative research team of high-level local universities in Shanghai and a key laboratory program of the Education Commission of Shanghai Municipality (ZDSYS14005).
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http://dx.doi.org/10.1016/j.ebiom.2021.103575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426208PMC
September 2021

Microwave ablation of benign thyroid nodules: 3-year follow-up outcomes.

Head Neck 2021 11 30;43(11):3437-3447. Epub 2021 Aug 30.

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Purpose: To evaluate the effectiveness of microwave ablation (MWA) for the treatment of thyroid nodules according to nodule composition.

Materials And Methods: This study evaluated 171 patients with 180 benign thyroid nodules (BTNs) that had been treated with ultrasound-guided MWA. The volume reduction rate (VRR) of thyroid nodules and factors, which had an influence on the VRR, were assessed. Therapeutic success was defined as a >50% volume reduction at the 12-month follow-up. Multivariate regression analysis was used to identify independent predictors of VRR for BTNs after MWA treatment.

Results: The mean diameter and volume of the nodules were 4.3 ± 1.3 cm and 18.9 ± 2.1 ml, respectively. The VRRs at the 1-, 3-, 6-, 12-, 24-, and 36-month follow-ups were 47.1%, 68.2%, 79.7%, 87.4%, 90.1%, and 93.2%, respectively. Of the 180 BTNs, there were 87 solid, 74 predominantly solid, and 19 predominantly cystic nodules. Solid nodules showed significantly lower VRRs compared with the predominantly solid and predominantly cystic nodules at the 1-, 3-, and 6-month follow-ups. For the multivariate regression analysis, the cyst component was an active prognostic factor for the VRR at the 1-, 3-, and 6-month follow-ups; the cyst component was not significantly associated with the VRR at the 12-, 24-, and 36-month follow-ups.

Conclusion: Our study suggested that ultrasound-guided MWA is an effective and safe procedure for the treatment of BTNs. Solid nodules indicate a lower VRR and less efficient than predominant solid nodules and predominant cystic nodules after MWA.
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http://dx.doi.org/10.1002/hed.26842DOI Listing
November 2021

Huaier granule prevents the recurrence of early-stage hepatocellular carcinoma after thermal ablation: A cohort study.

J Ethnopharmacol 2021 Dec 21;281:114539. Epub 2021 Aug 21.

Department of Gastrointestinal Surgery, Affiliated Tumor Hospital, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, 530021, PR China; Guangxi Clinical Research Center for Colorectal Cancer, Nanning, Guangxi Zhuang Autonomous Region, 530021, PR China. Electronic address:

Ethnopharmacological Relevance: Clinical trials have demonstrated that Trametes robinophila Murr (Huaier granule) can inhibit recurrence and metastasis after hepatocellular carcinoma (HCC) resection, but its efficacy as an adjuvant therapy after thermal ablation of early HCC is unknown.

Aim Of The Study: To analyze the prognostic value and side effects of Huaier granules in HCC patients undergoing thermal ablation.

Materials And Methods: Clinical information from 340 eligible subjects with early-stage HCC who were admitted to our department from September 1, 2008 to January 1, 2019 was extracted from the electronic medical record database. They were divided into the thermal ablation + TCM group and the thermal ablation group. Differences in their overall survival (OS), progression-free survival (PFS), extrahepatic metastatic rate (EMR), and therapeutic side effects (TSEs) between the two groups were compared. Beneficiaries of the integrated treatment and adequate treatment length were predicted.

Results: The median follow-up was 32.5 months (range 2-122 months). The 1-year, 3-year and 5-year OS rates in the integrated treatment group and the control group were 93.2% vs. 92.6%, 54.5% vs. 51.4%, 23.5% vs. 19.7% (p = 0.110, HR 0.76(0.54-1.07)). The 1-year, 3-year and 5-year PFS rates were 78.8% vs. 69.4%, 50.6% vs. 40.6%, 35.3% vs. 26.5%, respectively (p = 0.020, HR 0.67(0.48-0.94)). The median OS (35 vs. 31 months) and PFS (24 vs. 12.5 months) were longer in the integrated treatment group. The EMR in the integrated treatment group was significantly lower than that in the control group (p = 0.018, HR 0.49 (0.27-0.89)). Patients with any two of the following three factors might be predicted to be beneficiaries of the integrated treatment, including younger than 65 years (p =0.039, HR 0.70 (0.50-0.98)), single tumor (p = 0.035, HR 0.70 (0.50-0.98), and tumor size ≤3 cm (p = 0.029, HR 0.69 (0.50-0.96). Patients with continuous oral administration of TCM following ablation had a lower probability of recurrence and metastasis within 2 years (p = 0.015, HR 0.67 (0.49-0.93)). Although the integrated treatment group reported a higher incidence of nausea and emesis, there were no significant differences between the two groups.

Conclusion: TCM following ablation may prolong PFS and suppress recurrence in patients with HCC, with continuous oral administration for more than 2 years maybe experience a greater benefit. The TSEs of the treatment are mild and can be tolerated.
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http://dx.doi.org/10.1016/j.jep.2021.114539DOI Listing
December 2021

Enhanced removal of organoarsenic by chlorination: Kinetics, effect of humic acid, and adsorbable chlorinated organoarsenic.

J Hazard Mater 2022 01 3;422:126820. Epub 2021 Aug 3.

State Key Laboratory of Urban Water Resource and Environment, School of Environmental, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

In this study, the effective removal of organoarsenic by the combined process of "chlorination + Fe(II)" was achieved. Chlorine could effectively degrade roxarsone (ROX) over pH from 5 to 10. The fitting results of acid-base protonation model proved that the degradation of ROX was mainly attributed to the reaction of HOCl and deprotonated ROX. The transformation of arsenic species conformed to the fitting results of two-channel kinetic model, in which 32.4% of ROX was oxidized to As(V) via electron transfer pathway (ii) and the rest was converted into monochloro-ROX via electrophilic substitution pathway (i). Humic acid inhibited the degradation of ROX due to the competitive consumption of chlorine and the restraint on the pathway ii. Subsequently, an enhanced removal of total arsenic achieved after chlorination, due to that the generating As(V) and monochloro-ROX were easier adsorbed compared with ROX, over 97.8% of total arsenic was removed by ferric (oxyhydr)oxides which in-situ formed from the oxidation of Fe(II). Additionally, toxicity studies indicated that the acute toxicity was significantly eliminated by adding Fe(II) after chlorination, likely due to the removal of As(V) and chlorinated products. Furthermore, organoarsenic was also effectively removed by the combined process of "chlorination + Fe(II)" in real water.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126820DOI Listing
January 2022

Prognostic Value of miR-137 in Children with Medulloblastoma and its Regulatory Effect on Tumor Progression.

Neuromolecular Med 2021 Aug 18. Epub 2021 Aug 18.

Department of Neurosurgery, Children's Hospital of Chongqing Medical University, No. 136, 2nd Zhongshan Road, Yuzhong District, Chongqing, 400014, People's Republic of China.

Medulloblastoma is a malignant tumor with high incidence and poor prognosis in adolescents and children. MicroRNA-137 (miR-137) has been found to be abnormally expressed in cancers such as pancreatic cancer. The purpose of this study is to explore the expression of miR-137 in MB and its role in cell physiological activities to determine the significance of miR-137 in the prognosis of MB. First, the expression of miR-137 in MB tissues and cell lines was analyzed by qRT-PCR. Then the Kaplan-Meier survival curve was used to analyze the significance of miR-137 expression in the prognosis, and the Cox regression model was used to explore the correlation between miR-137 expression and clinical characteristics. The effects of miR-137 on MB cell activities were analyzed by MTT assay, Transwell assays, and flow cytometry. It can be concluded from the results that the expression of miR-137 is down-regulated in MB tissues and cells. The down-regulation of miR-137 was significantly related to the poor prognosis of MB, and significantly related to clinical indicators. Up-regulated miR-137 inhibited cell proliferation, migration, invasion, and cell cycle progression, as well as induced cell apoptosis by targeting KDM1A. This study can conclude that miR-137 may be used as a prognostic biomarker of MB.
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http://dx.doi.org/10.1007/s12017-021-08684-wDOI Listing
August 2021

A comprehensive analysis of gorilla-specific LINE-1 retrotransposons.

Genes Genomics 2021 Oct 18;43(10):1133-1141. Epub 2021 Aug 18.

Department of Microbiology, College of Science and Technology, Dankook University, Cheonan, 31116, Republic of Korea.

Background: Long interspersed element-1 (LINE-1 or L1) is the most abundant retrotransposons in the primate genome. They have approximately 520,000 copies and make up ~ 17% of the primate genome. Full-length L1s can mobilize to a new genomic location using their enzymatic machinery. Gorilla is the second closest species to humans after the chimpanzee, and human-gorilla split 7-12 million years ago. The gorilla genome provides an opportunity to explore primate origins and evolution.

Objective: L1s have contributed to genome diversity and variations during primate evolution. This study aimed to identify gorilla-specific L1s using a more recent version of the gorilla reference genome (Mar. 2016 GSMRT3/gorGor5).

Methods: We collected gorilla-specific L1 candidates through computational analysis and manual inspection. L1Xplorer was used to identify whether full-length gorilla-specific L1s were intact. In addition, to determine the level of sequence conservation between intact fulllength gorilla-specific L1s, two ORFs of intact L1s were aligned with the L1PA2 consensus sequence.

Results: 2002 gorilla-specific L1 candidates were identified through computational analysis. Among them, we manually inspected 1,883 gorilla-specific L1s, among which most of them belong to the L1PA2 subfamily and 12 were intact L1s that could influence genomic variations in the gorilla genome. Interestingly, the 12 intact full-length gorilla-specific L1s have 14 highly conserved nonsynonymous mutations, including 6 mutations and 8 mutations in ORF1 and ORF2, respectively. In comparison to the intact full-length chimpanzee-specific L1s and human-specific hot-L1s, two of these in ORF1 (L256F and E293G) were shown as gorilla-specific nonsynonymous mutations.

Conclusion: The gorilla-specific L1s may have had significantly affected the gorilla genome to compose a genome different form that of other primates during primate evolution.
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http://dx.doi.org/10.1007/s13258-021-01146-4DOI Listing
October 2021

Near-Infrared-Excited Multicolor Afterglow in Carbon Dots-Based Room-Temperature Afterglow Materials.

Angew Chem Int Ed Engl 2021 Oct 9;60(41):22253-22259. Epub 2021 Sep 9.

Key Laboratory for Biobased Materials and Energy of Ministry of Education/Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.

Room-temperature afterglow (RTA) materials with long lifetime have shown tremendous application prospects in many fields. However, there is no general design strategy to construct near-infrared (NIR)-excited multicolor RTA materials. Herein, we report a universal approach based on the efficient radiative energy transfer that supports the reabsorption from upconversion materials (UMs) to carbon dots-based RTA materials (CDAMs). Thus, the afterglow emission (blue, cyan, green, and orange) of various CDAMs can be activated by UMs under the NIR continuous-wave laser excitation. The efficient radiative energy transfer ensured the persistent multicolor afterglow up to 7 s, 6 s, 5 s, and 0.5 s by naked eyes, respectively. Given the unusual afterglow properties, we demonstrated preliminary applications in fingerprint recognition and information security. This work provides a new avenue for the activation of NIR-excited afterglow in CDAMs and will greatly expand the applications of RTA materials.
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http://dx.doi.org/10.1002/anie.202108696DOI Listing
October 2021

Patient-specific iPSC-derived endothelial cells reveal aberrant p38 MAPK signaling in atypical hemolytic uremic syndrome.

Stem Cell Reports 2021 Sep 12;16(9):2305-2319. Epub 2021 Aug 12.

Renal Division, Department of Medicine, Peking University First Hospital, 8 Xishiku Street, Beijing 100034, China; Peking University Institute of Nephrology, Beijing 100034, China; Key Laboratory of Renal Disease, Ministry of Health of China, Beijing 100034, China; Key Laboratory of Chronic Kidney Disease Prevention and Treatment, Ministry of Education of China, Beijing 100034, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100034, China.

Atypical hemolytic uremic syndrome (aHUS) is a rare disease associated with high morbidity and mortality. Existing evidence suggests that the central pathogenesis to aHUS might be endothelial cell damage. Nevertheless, the role of endothelial cell alterations in aHUS has not been well characterized and the underlying mechanisms remain unclear. Utilizing an induced pluripotent stem cell-derived endothelial cell (iPSC-EC) model, we showed that anti-complement factor H autoantibody-associated aHUS patient-specific iPSC-ECs exhibited an intrinsic defect in endothelial functions. Stimulation using aHUS serums exacerbated endothelial dysfunctions, leading to cell apoptosis in iPSC-ECs. Importantly, we identified p38 as a novel signaling pathway contributing to endothelial dysfunctions in aHUS. These results illustrate that iPSC-ECs can be a reliable model to recapitulate EC pathological features, thus providing a unique platform for gaining mechanistic insights into EC injury in aHUS. Our findings highlight that the p38 MAPK signaling pathway can be a therapeutic target for treatment of aHUS.
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http://dx.doi.org/10.1016/j.stemcr.2021.07.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452517PMC
September 2021

Dealloying-Derived Nanoporous CuSn Alloy as Stable Anode Materials for Lithium-Ion Batteries.

Materials (Basel) 2021 Aug 3;14(15). Epub 2021 Aug 3.

School of Applied Physics and Materials, Wuyi University, Jiangmen 529020, China.

The volume expansion during Li ion insertion/extraction remains an obstacle for the application of Sn-based anode in lithium ion-batteries. Herein, the nanoporous (np) CuSn alloy and CuSn/Sn composite were applied as a lithium-ion battery anode. The as-dealloyed np-CuSn has an ultrafine ligament size of 40 nm and a high BET-specific area of 15.9 m g. The anode shows an initial discharge capacity as high as 1200 mA h g, and it remains a capacity of higher than 600 mA h g for the initial five cycles at 0.1 A g. After 100 cycles, the anode maintains a stable capacity higher than 200 mA h g for at least 350 cycles, with outstanding Coulombic efficiency. The ex situ XRD patterns reveal the reverse phase transformation between CuSn and LiCuSn. The CuSn/Sn composite presents a similar cycling performance with a slightly inferior rate performance compared to np-CuSn. The study demonstrates that dealloyed nanoporous CuSn alloy could be a promising candidate for lithium-ion batteries.
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http://dx.doi.org/10.3390/ma14154348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346966PMC
August 2021

Generation of an induced pluripotent stem cell line from a patient carrying FBN1/c.6734 G > A mutation.

Stem Cell Res 2021 08 14;55:102459. Epub 2021 Jul 14.

Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China. Electronic address:

Mutations in FBN1 (gene encodes the matrix protein fibrillin 1), are usually associated with Marfan syndrome (MS). This syndrome contains ocular, skeletal, cardiovascular, pulmonary, cutaneous and neurological abnormalities. Here, we introduced an induced pluripotent stem cell (iPSC) line from a patient who suffered from severe cardiovascular disease and carried a c.6734G > A mutation in FBN1. Dermal fibroblasts of the patient were reprogrammed with non-integrating Sendai virus (SeV). Generated iPSC line exhibited normal karyotype, showed embryonic stem cell-like morphology, expressed pluripotency markers, and was capable of differentiating into three germ layers. This iPSC line will be a valuable tool for studying MS.
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http://dx.doi.org/10.1016/j.scr.2021.102459DOI Listing
August 2021

Nanoengineered biomimetic Cu-based nanoparticles for multifunational and efficient tumor treatment.

Biomaterials 2021 09 8;276:121016. Epub 2021 Jul 8.

Laboratory of Controllable Preparation and Application of Nanomaterials, CAS Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29 East Road Zhongguancun, Beijing, 100190, People's Republic of China. Electronic address:

The microwave dynamic therapy (MDT) mediated by cytotoxic reactive oxygen species (ROS) is a promising anticancer therapeutic method. However, the therapeutic efficiency of MDT is restricted by several limitations including insufficient ROS generation, strong proangiogenic response, and low tumor-targeting efficiency. Herein, we find that Cu-based nanoparticles can produce oxygen under microwave (MW) irradiation to raise the generation of ROS, such as •O, •OH and O, especially •O. On this basis, a nanoengineered biomimetic strategy is designed to improve the efficiency of MDT. After intravenous administration, the nanoparticles accumulate to the tumor site through targeting effect mediated by biomimetic modification, and it can continuously produce oxygen to raise the levels of ROS in tumor microenvironment under MW irradiation for MDT. Additionally, Apatinib is incorporated as antiangiogenic drug to downregulate the expression of vascular endothelial growth factor (VEGF), which can effectively inhibit the tumor angiogenesis after MDT. Hence, the tumor inhibition rate is as high as 96.79%. This study provides emerging strategies to develop multifunctional nanosystems for efficient tumor therapy by MDT.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121016DOI Listing
September 2021

Colonic metastasis from hepatocellular carcinoma after treated by ablation and transarterial chemoembolization manifested by intestinal obstruction: A case report and review of the literature.

J Cancer Res Ther 2021 Jul;17(3):814-817

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, China.

Hepatocellular carcinoma (HCC) with gastrointestinal tract metastasis is a rare condition. Recently, we encountered one case of HCC with direct invasion to the colon, which was manifested by intestinal obstruction. The patient was an 86-year-old man who underwent multiple transarterial chemoembolization and microwave ablation treatment for HCC lesions for 10 years. Two months after last palliative microwave ablation, computed tomography showed a 7.0-cm liver tumor directly invading the hepatic flexure of colon with the symptom of right abdominal pain and discontinuous nausea and vomiting. HCC colon metastasis with intestinal obstruction was diagnosed. Intestinal adhesion lysis and colostomy were performed. The patient survived 10 months after surgery and died of tumor progression.
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http://dx.doi.org/10.4103/jcrt.JCRT_217_20DOI Listing
July 2021

Ultrasound-guided percutaneous microwave ablation of bilateral renal cell carcinomas: Focus upon safety and efficacy.

J Cancer Res Ther 2021 Jul;17(3):803-807

Department of Interventional Ultrasound, Chinese PLA Medical School, Beijing, China.

Objective: To retrospectively review the safety and efficacy of ultrasound (US)-guided percutaneous microwave ablation (MWA) of bilateral renal cell carcinomas (BRCCs).

Patients And Methods: In this study, eight patients of BRCCs with 20 lesions seen from November 2012 to October 2018 were enrolled retrospectively. The patients were treated with US -guided percutaneous MWA and were followed up with contrast-enhanced US and computed tomography or magnetic resonance imaging.

Results: Technical success was achieved 20 tumors. The 18 lesions were performed to ablate 90%; 2 lesions were repeated because of detecting residual ablated tumor. Except six patients were noted fever, no severe complications occurred. The median follow-up time was 24 months (range 7-59 months). Among the eight patients, there was no local tumor recurrence in 6 of 8 completely ablated tumor lesions. One patient was retreated by MWA 6 months after ablation, and another was retreated after 19 months due to local tumor recurrence. No patients have obvious change of renal function and lost their life.

Conclusion: US-guided percutaneous MWA is a beneficial treatment for BRCC.
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http://dx.doi.org/10.4103/jcrt.jcrt_1906_20DOI Listing
July 2021

BCL6B hypermethylation predicts metastasis and poor prognosis in early-stage hepatocellular carcinoma after thermal ablation.

J Cancer Res Ther 2021 Jul;17(3):644-651

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Aims: The aim of this study was to evaluate the role of BCL6B methylation in the progression of early-stage hepatocellular carcinoma (HCC) after thermal ablation.

Settings And Design: This is a retrospective study and written informed consent was obtained from all patients or their legal guardians.

Subjects And Methods: Between October 2008 and December 2013, 73 patients with early-stage HCC within the Milan criteria, who received thermal ablation, were recruited.

Statistical Analysis Used: Based on methylation-specific polymerase chain reaction, the relationship between BCL6B methylation and patient characteristics and prognosis was analyzed using univariate, multivariate, and Kaplan-Meier analysis.

Results: The median follow-up period was 56 (8-110) months. For the BCL6B unmethylated group, the 1-, 3- and 5-year metastasis and overall survival (OS) rates after thermal ablation were 10.0%, 10.0%, and 40.0% and 100%, 100% and 90.0%, respectively. The 1-, 3-, and 5-year metastasis and OS rates of the methylated group were 23.8%, 66.7% and 88.9% and 66.2%, 71.4% and 41.3%, respectively. Levels of absolute count lymphocyte, serum cholinesterase and albumin in the BCL6B unmethylated group were higher than those in the methylated group (P = 0.020, 0.000, and 0.009, respectively). Kaplan-Meier analysis revealed that BCL6B methylation was related to metastasis and poor prognosis (P = 0.001 and 0.018, respectively). Univariate analysis revealed that BCL6B methylation was a risk factor for metastasis and poor prognosis (odds ratio [OR]: 5.663; 95% confidence interval [CI], 1.745-18.375, P = 0.004 and OR: 3.734; 95% CI, 1.151-12.110, P = 0.028, respectively). Multivariate analysis revealed that BCL6B methylation was an independent risk factor for metastasis (OR: 3.736; 95% CI, 1.000-13.963,P = 0.05) and not for prognosis (OR: 2.780; 95% CI, 0.835-9.250,P = 0.096).

Conclusions: BCL6B methylation could be a valuable prognostic factor for metastasis and poor prognosis in early-stage HCC after thermal ablation, which is an independent risk factor for metastasis. Our findings provide insights for combining ablation and epigenetic therapy for patients with HCC.
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http://dx.doi.org/10.4103/jcrt.JCRT_1504_20DOI Listing
July 2021

Percutaneous microwave ablation versus robot-assisted hepatectomy for early hepatocellular carcinoma: A real-world single-center study.

Dig Liver Dis 2021 Jul 6. Epub 2021 Jul 6.

Department of Interventional Ultrasound, The First Center of Chinese PLA General Hospital, Address:28 Fuxing Road, Beijing, 100853 China. Electronic address:

Background: Both microwave ablation and robot-assisted hepatectomy are representative minimally invasive treatments for early hepatocellular carcinoma. Our study compares the practicability and medium-term therapeutic efficacy between them.

Methods: Patients with early HCC treated by MWA or RH from 2013 to 2019 were included. Propensity score matching (PSM) and inverse probability of treatment weight (IPTW) were used to minimize baseline imbalance. Operation trauma, postoperative recovery, complications, cost and oncological efficacy were compared.

Results: 401 patients with a median follow-up of 28 months were included (MWA n = 240; RH n = 161). After PSM, 3-year recurrence-free survival (RFS), overall survival (OS) and cancer-specific survival (CSS) of MWA group and RH group were 52.2% vs 65.8%, 91.5% vs 91.3% and 91.5% vs 91.3%, respectively. OS and CSS were comparable (p = 0.44 and 0.96), while RFS of MWA was slightly lower but not significant (p = 0.097). The above results after IPTW followed the same trend. After PSM, MWA showed advantages in operation time and blood loss, while RH performed better in postoperative liver function. There was no significant difference in incidence of severe complications between two groups.

Conclusions: For early HCC parents, both treatments can achieve good, safe and comparable medium-term therapeutic effects. MWA is more minimally invasive, while RH has better accuracy and causes less damage to liver parenchyma.
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http://dx.doi.org/10.1016/j.dld.2021.04.008DOI Listing
July 2021

A multicenter 10-year oncologic outcome of ultrasound-guided percutaneous microwave ablation of clinical T1 renal cell carcinoma: will it stand the test of time?

Eur Radiol 2021 Jun 30. Epub 2021 Jun 30.

Department of Interventional Ultrasound, PLA Medical College & Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China.

Objectives: We updated the experience on percutaneous microwave ablation for renal cell carcinoma with five-center data and long-term follow-up.

Methods: This retrospective study reviewed the T1N0M0 renal cell carcinoma patients who underwent microwave ablation between April 2006 and December 2019. Clinicopathological and procedural data were collected. Technical effectiveness and complications were assessed, and the Kaplan-Meier method was used for cancer-specific survival, disease-free survival, overall survival, and local neoplastic process analyses.

Results: A total of 323 consecutive patients (mean age, 62.9 years ± 14.0) with 371 biopsy-proved tumors (mean diameter, 2.9 cm ± 1.2) were enrolled, and 42.6% of the tumors were located adjacent to collecting system/bowel and technical effectiveness was achieved in 360 (97.0%) tumors. For 275 cT1a patients, during median follow-up time of 66.0 months (IQR, 58.4-73.6), 10-year local neoplastic processes, cancer-specific survival, disease-free survival, and overall survival rates were 1.9%, 87.4%, 71.8, and 67.5%, respectively. For 48 cT1b patients, during the median follow-up time of 30.4 months (IQR, 17.7-44.8), 5-year local tumor progression, cancer-specific survival, disease-free survival, and overall survival rates were 11.3%, 91.4%, 69.1, and 89.2%, respectively. Major complications showed no differences between cT1a (3.5%) and cT1b (6.9%) patients (p = 0.28). A clinical risk stratification system was developed based on multivariable model to predict DFS and CSS with c-indexes of 0.78 (95% CI: 0.71-0.85) and 0.77 (95% CI: 0.65-0.90), respectively.

Conclusions: With matured follow-up at five institutions, ultrasound-guided percutaneous microwave ablation is a reliable treatment option for cT1a renal cell carcinoma even in dangerous location and appears to be promising for cT1b tumors.

Key Points: • To our knowledge, this is the first multicenter cohort of long-term oncologic outcomes with percutaneous MWA of cT1 RCC. • The predicting model we developed is accurate to predict the long-term DFS and CSS, which can help to provide a better MWA prognostication over routinely available clinical information. • The available evidence shows that microwave ablation of clinical stage T1 RCC is safe and reliable with promising long-term oncologic outcomes, especially for cT1a RCC with excellent 10-year results.
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http://dx.doi.org/10.1007/s00330-021-07900-2DOI Listing
June 2021
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