Publications by authors named "Ping Li"

5,128 Publications

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Characterization of chemical constituents and metabolites in rat plasma after oral administration of San Miao Wan by ultra-high performance liquid chromatography tandem Q-Exactive Orbitrap mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 May 28;1178:122793. Epub 2021 May 28.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, Jiangsu Province, PR China; Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, Jiangsu Province, PR China. Electronic address:

San Miao Wan (SMW), composed of Phellodendri Chinensis Cortex, Atractylodis Lanceae Rhizoma and Achyranthis Bidentatae Radix, is widely used for the treatment of gout, hyperuricemia and other diseases. In the present study, an overall identification strategy based on ultra-high performance liquid chromatography tandem Q-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap/MS) method was established to characterize the multiple chemical constituents of SMW and its metabolites in rat plasma after oral administration of SMW. A total of 76 constituents including alkaloids, organic acids, lactones, terpenes, saponins, sterones and others types of components were identified in the extract of SMW. After the oral administration of SMW, 47 prototype constituents and 66 metabolites were identified in rat plasma samples. The related metabolic pathways mainly involved reduction, demethylation, hydroxylation, methylation and glucuronide conjunction. The proposed method could be a useful approach to identify the chemical constituents of SMW and its metabolic components. Our study provide a universal strategy for the analysis of the components and metabolites of the traditional Chinese medicine prescription (TCP) extracts and plasma after administration using UPLC-Q-Exactive Orbitrap/MS method. It will assist with clarifying the substance basis of effective components in SMW. It also provides a rapid method for overall analysis of chemical constituents and metabolites of SMW.
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http://dx.doi.org/10.1016/j.jchromb.2021.122793DOI Listing
May 2021

The receptor-like cytoplasmic kinase RIPK regulates broad-spectrum ROS signaling in multiple layers of plant immune system.

Mol Plant 2021 Jun 12. Epub 2021 Jun 12.

Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address:

Production of reactive oxygen species (ROS) via the activity of respiratory burst oxidase homologs (RBOHs) plays a vital role in multiple layers of the plant immune system, including pathogen-associated molecular pattern-triggered immunity (PTI), damage-associated molecular pattern-triggered immunity (DTI), effector-triggered immunity (ETI) and systemic acquired resistance (SAR). It is generally established that RBOHD was activated by different receptor-like cytoplasmic kinases (RLCKs) in responses to different immune elicitors. In this study, we found that RPM1-INDUCED PROTEIN KINASE (RIPK), an RLCK VII subfamily member, contributes to ROS production in multiple layers of the plant immune system. ripk mutants showed reduced ROS production in response to treatment with all examined immune elicitors that trigger PTI, DTI, ETI, and SAR, respectively. We found that RIPK can directly phosphorylate the N-terminal region of RBOHD in vitro, and the levels of phosphorylated S343/S347 residues of RBOHD were reduced in ripk mutants upon treatment with all immune elicitors. Phosphorylation of RIPK was required for its function in regulating RBOHD-mediated ROS production. Similar to rbohd, ripk mutants showed reduced stomatal closure, impaired SAR, and were susceptible to the necrotrophic bacterium Pectobacterium carotovorum. Collectively, our results indicate that RIPK regulates broad-spectrum RBOHD-mediated ROS signaling during PTI, DTI, ETI, and SAR, leading to subsequent RBOHD-dependent immune responses.
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http://dx.doi.org/10.1016/j.molp.2021.06.010DOI Listing
June 2021

Indoor simulation test research on cumulative longitudinal displacement of rail based on force and displacement sensors data collection.

Sci Prog 2021 Apr-Jun;104(2):368504211023287

School of Civil Engineering, Central South University, Changsha, Hunan, China.

The train sometimes needs to brake frequently on the turnout, although the braking force does not exceed the limit resistance of fastener, cumulative displacement of rail occurs because of the long-term effect of the train brakes, thus, the relationship between the cumulative displacement of rail and the number of train braking actions should be explored. Aiming at the spring bar type III fastener, a 1:1 physical indoor simulation test was carried out, and an electromagnetic relay device was used to simulate the train load, force, and displacement sensors for data collection. Then a single load no more than the maximum resistance of fastener was applied to the rail end to explore the relationship between the number of loads and the rail cumulative deformation. The rail longitudinal cumulative displacement changes linearly in positive correlation with the number of load actions, and increases faster when the number of load actions is small. As the number of repeated loads increases, the above-mentioned relationship approximately and credibly obeys the power function distribution. Repeatedly applying load no more than the maximum longitudinal resistance of fastener to the rail, the existence of the rail cumulative displacement caused by frequent train braking can be demonstrated, and the relationship curve between the rail displacement and the number of loads can be obtained. Applying the fitting formula, the rail displacement after a specific number of loading times can be attained, and then referring to specific codes, we can determine whether it will exceed the safety limit.
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http://dx.doi.org/10.1177/00368504211023287DOI Listing
June 2021

degradation, biocompatibility and antibacterial properties of pure zinc: assessing the potential of Zn as a guided bone regeneration membrane.

J Mater Chem B 2021 Jun 15. Epub 2021 Jun 15.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.

Membrane exposure is a common complication after the guided bone regeneration (GBR) procedure and has a detrimental influence on the bone regeneration outcomes, while the commercially available GBR membranes show limited exposure tolerance. Recently, zinc (Zn) has been suggested as a promising material to be used as a barrier membrane in GBR therapy for bone augmentation. In this study, the degradation behavior in artificial saliva solution, cytotoxicity and antibacterial activity of pure Zn were investigated to explore its degradation and associated biocompatibility in the case of premature membrane exposure. The results indicated that the degradation rate of Zn in artificial saliva solution was about 31.42 μm year-1 after 28 days of immersion. The corrosion products on the Zn surface were mainly composed of Zn3(PO4)2, Ca3(PO4)2, CaHPO4, Zn5(CO3)2(OH)6 and ZnO. Besides, Zn presented an acceptable in vitro HGF cytocompatibility and a high antibacterial activity against Porphyromonas gingivalis. The preliminary results demonstrate that pure Zn exhibits appropriate degradation behavior, adequate cell compatibility and favorable antibacterial properties in the oral environment and is thus believed to sustain profitable function when membrane exposure occurs. The results provided new insights for understanding the exposure tolerance of Zn based membranes and are beneficial to their clinical applications.
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http://dx.doi.org/10.1039/d1tb00596kDOI Listing
June 2021

RNA and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16.

BMC Genomics 2021 Jun 15;22(1):444. Epub 2021 Jun 15.

Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931, Cologne, Germany.

Background: Autophagy is an evolutionary ancient mechanism that sequesters substrates for degradation within autolysosomes. The process is driven by many autophagy-related (ATG) proteins, including the core members ATG9 and ATG16. However, the functions of these two core ATG proteins still need further elucidation. Here, we applied RNA and tandem mass tag (TMT) proteomic approaches to identify differentially expressed genes (DEGs) and proteins (DEPs) in Dictyostelium discoideum ATG9‾, ATG16‾ and ATG9‾/16‾ strains in comparison to AX2 wild-type cells.

Result: In total, we identified 332 (279 up and 53 down), 639 (487 up and 152 down) and 260 (114 up and 146 down) DEGs and 124 (83 up and 41 down), 431 (238 up and 193 down) and 677 (347 up and 330 down) DEPs in ATG9‾, ATG16‾ and ATG9‾/16‾ strains, respectively. Thus, in the single knock-out strains, the number of DEGs was higher than the number of DEPs while in the double knock-out strain the number of DEPs was higher. Comparison of RNA and proteomic data further revealed, that only a small proportion of the transcriptional changes were reflected on the protein level. Gene ontology (GO) analysis revealed an enrichment of DEPs involved in lipid metabolism and oxidative phosphorylation. Furthermore, we found increased expression of the anti-oxidant enzymes glutathione reductase (gsr) and catalase A (catA) in ATG16‾ and ATG9‾/16‾ cells, respectively, indicating adaptation to excess reactive oxygen species (ROS).

Conclusions: Our study provides the first combined transcriptome and proteome analysis of ATG9‾, ATG16‾ and ATG9‾/16‾ cells. Our results suggest, that most changes in protein abundance were not caused by transcriptional changes, but were rather due to changes in protein homeostasis. In particular, knock-out of atg9 and/or atg16 appears to cause dysregulation of lipid metabolism and oxidative phosphorylation.
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http://dx.doi.org/10.1186/s12864-021-07756-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204557PMC
June 2021

Evidences from infrared and Raman spectra: Xiaomeiling is one reasonable provenance of nephrite materials used in Liangzhu Culture.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 25;261:120012. Epub 2021 May 25.

School of Culture and Tourism, Guizhou Education University, 550018 Guiyang, China.

The Raman and infrared spectra of fresh Xiaomeiling nephrite are investigated and compared with Neolithic nephrite artifacts from the lower reaches of Yangtze River. An extensive comparison shows that these nephrite artifacts probably come from the same nephrite deposit and the spectral characteristics of Xiaomeiling nephrite are highly consistent with these nephrite artifacts, especially from Liangzhu Culture. Other characteristics of Xiaomeiling nephrite, such as primary colors, chemical compositions and rock structures, also mostly coincide with these nephrite artifacts. Combined with the major element compositions of Xiaomeiling nephrite and the nephrite artifacts from Liangzhu Culture, the coupling effect of Na-Al dominated by short-range order of cations in Raman and infrared spectra is discussed in detail. Take into account these evidences, Xiaomieling as one significant provenance of nephrite materials used in Liangzhu Culture ought not to be excluded, although perhaps not a unique source.
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http://dx.doi.org/10.1016/j.saa.2021.120012DOI Listing
May 2021

Identification of Two Novel Compound Heterozygous Mutations Underlying Wolcott-Rallison Syndrome in a Chinese Family.

Front Pediatr 2021 26;9:679646. Epub 2021 May 26.

Institutes of Biomedical Sciences, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan, China.

Wolcott-Rallison syndrome is a rare autosomal recessive inheritance disorder caused by the defectiveness of eukaryotic translation initiation factor 2 alpha kinase 3 (), which encodes the PKR-like endoplasmic reticulum kinase (PERK). Defect in results in a permanent diabetes in early infancy or newborn period, a tendency to develop skeletal fractures and other associated disorders such as severe liver and renal dysfunction, and central hypothyroidism. Two patients with Wolcott-Rallison syndrome-like manifestations in a Chinese family and family members were genetically analyzed to identify if any variations that occurred in , which may cause Wolcott-Rallison syndrome. Whole-exome sequencing (WES) was performed to identify genetic variations, and Sanger sequencing was conducted to verify the identified variations in the family members with Wolcott-Rallison syndrome (WRS) clinical manifestations. Several bioinformatics tools were employed to predict the effect of variations on the protein function. The impact on PERK protein was analyzed by sequential analysis and evolution conservation study. Two novel heterozygous single base variations (c.2818C>T and c.2980G>C) were detected in the proband. PERK has two functional domains: one is regulatory domain (aa 1-576), and the other is catalytic domain (aa 577-1,115). Both variations are missense mutations and locate in catalytic domain of PERK; c.2818C>T resulted in a residue substitution of proline for serine at amino acid site 940 (p.Pro940Ser), and variation c.2980G>C caused an amino acid change at position 994 from glutamic acid to glutamine (p.Glu994Gln). These novel missense variations may affect the physiological functions of PERK protein. Two novel compound heterozygous variations (c.2818C>T, p.Pro940Ser and c.2980G>C, p.Glu994Gln) were found in a Chinese family. The identification of the variations and verification of their pathogenicity extended the variation spectrum of variations causing Wolcott-Rallison syndrome and enriched valuable information for precise medical intervention for Wolcott-Rallison syndrome in China.
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http://dx.doi.org/10.3389/fped.2021.679646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187601PMC
May 2021

Large transition state stabilization from a weak hydrogen bond.

Chem Sci 2020 Jul 2;11(28):7487-7494. Epub 2020 Jul 2.

Department of Chemistry and Biochemistry, University of South Carolina Columbia SC 29208 USA

A series of molecular rotors was designed to study and measure the rate accelerating effects of an intramolecular hydrogen bond. The rotors form a weak neutral O-H⋯O[double bond, length as m-dash]C hydrogen bond in the planar transition state (TS) of the bond rotation process. The rotational barrier of the hydrogen bonding rotors was dramatically lower (9.9 kcal mol) than control rotors which could not form hydrogen bonds. The magnitude of the stabilization was significantly larger than predicted based on the independently measured strength of a similar O-H⋯O[double bond, length as m-dash]C hydrogen bond (1.5 kcal mol). The origins of the large transition state stabilization were studied experimental substituent effect and computational perturbation analyses. Energy decomposition analysis of the hydrogen bonding interaction revealed a significant reduction in the repulsive component of the hydrogen bonding interaction. The rigid framework of the molecular rotors positions and preorganizes the interacting groups in the transition state. This study demonstrates that with proper design a single hydrogen bond can lead to a TS stabilization that is greater than the intrinsic interaction energy, which has applications in catalyst design and in the study of enzyme mechanisms.
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http://dx.doi.org/10.1039/d0sc02806aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159443PMC
July 2020

Benzaldehyde, A New Absorption Promoter, Accelerating Absorption on Low Bioavailability Drugs Through Membrane Permeability.

Front Pharmacol 2021 28;12:663743. Epub 2021 May 28.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Styrax, one of the most famous folk medicines, is a necessary medicine in formulas to help other drugs reach the focal zone and maximize the effectiveness, the mechanism that promotes absorption is not clear yet. This study was carried out to investigate the absorption-promoting effects and the mechanism of benzaldehyde, a key active compound of styrax, on the diffusion rates of drugs with different oral bioavailability. Caco-2 transport experiments were used to investigate the transport rate. Molecular Dynamics Simulation analysis and fluorescence-anisotropy measurements were used to explore the underlying mechanism of absorption-promoting. Validation test was carried out to reveal the absorption-promoting effects of benzaldehyde on high hydrophilicity drugs. Our data indicated that benzaldehyde(50 μM) elevated the cumulative quantity of passively diffusion drugs with high hydrophilicity such as acyclovir and hydrochlorothiazide. MD and membrane fluidity data explained that benzaldehyde can loosen the structure of the lipid bilayer. The validation tests showed that benzaldehyde (140 mg/kg) remarkably increased the C and AUC0-6 of acyclovir and hydrochlorothiazide . These present studies suggested that benzaldehyde can promote the absorption of drugs with a lower oral bioavailability through disturbing the integrity of lipid bilayer enhanced membrane permeability.
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http://dx.doi.org/10.3389/fphar.2021.663743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194254PMC
May 2021

Epidemiological feature, viral shedding, and antibody seroconversion among asymptomatic SARS-CoV-2 carriers and symptomatic/presymptomatic COVID-19 patients.

J Infect Public Health 2021 May 27;14(7):845-851. Epub 2021 May 27.

Department of Epidemiology, Second Military Medical University, Shanghai 200433, China. Electronic address:

Background: Novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pandemic. However, data concerning the epidemiological features, viral shedding, and antibody dynamics between asymptomatic SARS-CoV-2 carriers and COVID-19 patients remain controversial.

Methods: We enrolled 193 SARS-CoV-2 infected subjects in Ningbo and Zhoushan, Zhejiang, China, from January 21 to March 6, 2020. All subjects were followed up to monitor the dynamics of serum antibody immunoglobulin M (IgM) and IgG against SARS-CoV-2 using colloidal gold-labeled and enzyme-linked immunosorbent assays.

Results: Of those, 31 were asymptomatic SARS-CoV-2 carriers, 148 symptomatic COVID-19 patients, and 14 presymptomatic COVID-19 patients. Compared to symptomatic COVID-19 patients, asymptomatic carriers were younger and had higher levels of white blood cell and lymphocyte, lower level of C-reactive protein, and shorter viral shedding duration. Conversion of IgM from positive to negative was shorter in asymptomatic carriers than in COVID-19 patients (7.5 vs. 25.5 days, P = 0.030). The proportion of those persistently seropositive for IgG against SARS-CoV-2 was higher in COVID-19 patients than in asymptomatic carriers (66.1% vs. 33.3%, P = 0.037). Viral load was higher in symptomatic patients than presymptomatic patients (P = 0.003) and asymptomatic carriers (P = 0.004). Viral shedding duration was longer in presymptomatic COVID-19 patients than in asymptomatic carriers (48.0 vs. 24.0 days, P = 0.002). Asymptomatic carriers acquired infection more from intra-familial transmission than did COVID-19 patients (89.0% vs. 61.0%, P = 0.028). In 4 familial clusters of SARS-CoV-2 infection, asymptomatic carriers were mainly children and young adults while severe COVID-19 was mainly found in family members older than 60 years with comorbidities.

Conclusion: Asymptomatic carriers might have a higher antiviral immunity to clear SARS-CoV-2 than symptomatic COVID-19 patients and this antiviral immunity should be contributable to innate and adaptive cellular immunity rather than humoral immunity. The severity of COVID-19 is associated with older age and comorbidities in familial clustering cases.
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http://dx.doi.org/10.1016/j.jiph.2021.05.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154191PMC
May 2021

Maternal smoking during pregnancy and risks to depression and anxiety in offspring: An observational study and genome-wide gene-environment interaction analysis in UK biobank cohort.

J Psychiatr Res 2021 May 27;140:149-158. Epub 2021 May 27.

Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, China. Electronic address:

Background: Maternal smoking during pregnancy (MSDP) has been reported to be associated with increased anxiety and depression behaviors in offspring. However, there is still scant evidence to support the link between MSDP and anxiety/depression.

Methods: Using the subjects from the UK Biobank cohort (n = 371,903-432,881). Logistic regression analyses were first conducted to test the correlation between MSDP and anxiety/depression in offspring. Second, genome-wide gene-environment interaction study (GWGEIS) analyses were conducted by PLINK, using MSDP as environmental factor. Genetic correlation analysis of anxiety/depression and smoking was conducted by the LDSC software using the published genome-wide association study (GWAS) summary data of four smoking traits (n = 337,334-1,232,091), anxiety (n = 31,880) and depression (n = 490,359). Finally, pathway enrichment analysis was carried out to detect the pathway involved in the development of offspring anxiety caused by the interaction of MSDP × SNPs.

Results: Observational analyses showed that anxiety and depression status in offspring were significantly associated with MSDP (all p < 0.0001). Further GWEGI analyses observed significant MSDP-gene interaction effects at UNC80 gene for anxiety (p = 9.09 × 10). LDSC did not detect significant genetic correlation between anxiety and smoking traits. Pathway analysis identified 19 significant pathways for anxiety, such as MANALO_HYPOXIA_UP (FDR = 5.50 × 10), REACTOME_ADHERENS_JUNCTIONS_INTERACTIONS (FDR = 0.0304) and ONDER_CDH1_TARGETS_2_UP (FDR = 0.0371).

Conclusion: Our study results suggested the important impact of MDSP on the risk of anxiety in offspring, partly attributing to environment-gene interactions effects.
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http://dx.doi.org/10.1016/j.jpsychires.2021.05.067DOI Listing
May 2021

Integrated bioinformatics analysis and screening of hub genes in papillary thyroid carcinoma.

PLoS One 2021 11;16(6):e0251962. Epub 2021 Jun 11.

Department of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, PR China.

Background: With the increasing incidence of papillary thyroid carcinoma (PTC), PTC continues to garner attention worldwide; however its pathogenesis remains to be elucidated. The purpose of this study was to explore key biomarkers and potential new therapeutic targets for, PTC.

Methods: GEO2R and Venn online software were used for screening of differentially expressed genes. Hub genes were screened via STRING and Cytoscape, followed by Gene Ontology and KEGG enrichment analysis. Finally, survival analysis and expression validation were performed using the UALCAN online software and immunohistochemistry.

Results: We identified 334 consistently differentially expressed genes (DEGs) comprising 136 upregulated and 198 downregulated genes. Gene Ontology enrichment analysis results suggested that the DEGs were mainly enriched in cancer-related pathways and functions. PPI network visualization was performed and 17 upregulated and 13 downregulated DEGs were selected. Finally, the expression verification and overall survival analysis conducted using the Gene Expression Profiling Interactive Analysis Tool (GEPIA) and UALCAN showed that LPAR5, TFPI, and ENTPD1 were associated with the development of PTC and the prognosis of PTC patients, and the expression of LPAR5, TFPI and ENTPD1 was verified using a tissue chip.

Conclusions: In summary, the hub genes and pathways identified in the present study not only provide information for the development of new biomarkers for PTC but will also be useful for elucidation of the pathogenesis of PTC.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251962PLOS
June 2021

Identification of Susceptible Genes for Chronic Obstructive Pulmonary Disease with Lung Adenocarcinoma by Weighted Gene Co-Expression Network Analysis.

Onco Targets Ther 2021 4;14:3625-3634. Epub 2021 Jun 4.

Department of Respiratory and Critical Care Medicine, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.

Purpose: Chronic obstructive pulmonary disease (COPD) and lung adenocarcinoma (LUAD) are common disorders and usually co-exists. However, genetic mechanisms between COPD and LUAD are rarely reported. This study aims to identify susceptible genes of COPD with LUAD.

Methods: Using the published data of GSE106899, co-expression modules were constructed by weighted gene co-expression network analysis (WGCNA). Subsequently, top 50 genes in the most tumor-related module were identified, among which hub genes were selected and validated.

Results: Twenty co-expression modules were constructed on 13,865 genes from 62 lung tissues of COPD patients with or without LUAD, in which one module (blue) was most related to tumorigenesis. Functional enrichment analyses showed that the genes in the blue module were mainly enriched in cell cycle, DNA transcription/replication and cancer pathways, etc. Combined with protein-protein interaction network, MTA1, PKMYT1 and FZR1 genes had the most intramodular connectivity, which were regarded as the hub genes. However, only FZR1 was validated to be overexpressed in lung tissues of COPD with LUAD and cigarette smoke extract-stimulated A549 cells, a human LUAD cell line.

Conclusion: This study suggests overexpression of FZR1 may play a key role in the tumorigenesis of LUAD in patients with COPD.
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http://dx.doi.org/10.2147/OTT.S303544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187107PMC
June 2021

FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling.

Cell Death Dis 2021 Jun 10;12(6):602. Epub 2021 Jun 10.

Department of Central Lab, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

FKBP4 belongs to the family of immunophilins, which serve as a regulator for steroid receptor activity. Thus, FKBP4 has been recognized to play a critical role in several hormone-dependent cancers, including breast and prostate cancer. However, there is still no research to address the role of FKBP4 on lung adenocarcinoma (LUAD) progression. We found that FKBP4 expression was elevated in LUAD samples and predicted significantly shorter overall survival based on TCGA and our cohort of LUAD patients. Furthermore, FKBP4 robustly increased the proliferation, metastasis, and invasion of LUAD in vitro and vivo. Mechanistic studies revealed the interaction between FKBP4 and IKK kinase complex. We found that FKBP4 potentiated IKK kinase activity by interacting with Hsp90 and IKK subunits and promoting Hsp90/IKK association. Also, FKBP4 promotes the binding of IKKγ to IKKβ, which supported the facilitation role in IKK complex assembly. We further identified that FKBP4 TPR domains are essential for FKBP4/IKK interaction since its association with Hsp90 is required. In addition, FKBP4 PPIase domains are involved in FKBP4/IKKγ interaction. Interestingly, the association between FKBP4 and Hsp70/RelA favors the transport of RelA toward the nucleus. Collectively, FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to potentiate the transcriptional activity and nuclear translocation of NF-κB, thereby promoting LUAD progression. Our findings suggest that FKBP4 may function as a prognostic biomarker of LUAD and provide a newly mechanistic insight into modulating IKK/NF-κB signaling.
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http://dx.doi.org/10.1038/s41419-021-03857-8DOI Listing
June 2021

Mapping-by-sequencing the locus of EMS-induced mutation responsible for tufted-fuzzless seed phenotype in cotton.

Mol Genet Genomics 2021 Jun 10. Epub 2021 Jun 10.

Cotton Fiber Bioscience Research Unit, USDA-ARS-SRRC, New Orleans, LA, 70124, USA.

Cotton fiber mutants are valuable resources for studying functions of altered genes and their roles in fiber development. The n is a recessive tufted-fuzzless seed mutant created through chemical mutagenesis with ethyl methanesulfonate. Genetic analysis indicated that the tufted-fuzzless phenotype is controlled by a single recessive locus. In this study, we developed an F population of 602 progeny plants and sequenced the genomes of the parents and two DNA bulks from F progenies showing the mutant phenotype. We identified DNA sequence variants between the tufted-fuzzless mutant and wild type by aligning the sequence reads to the reference TM-1 genome and designed subgenome-specific SNP markers. We mapped the n locus on chromosome D04 within a genomic interval of about 411 kb. In this region, seven genes showed significant differential expression between the tufted-fuzzless mutant and wild type. Possible candidate genes are discussed in this study. The utilization of the n mutant along with other fiber mutants will facilitate our understanding of the molecular mechanisms of cotton fiber cell growth and development.
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http://dx.doi.org/10.1007/s00438-021-01802-0DOI Listing
June 2021

NHBS-Net: A Feature Fusion Attention Network for Ultrasound Neonatal Hip Bone Segmentation.

IEEE Trans Med Imaging 2021 Jun 9;PP. Epub 2021 Jun 9.

Ultrasound is a widely used technology for diagnosing developmental dysplasia of the hip (DDH) because it does not use radiation. Due to its low cost and convenience, 2-D ultrasound is still the most common examination in DDH diagnosis. In clinical usage, the complexity of both ultrasound image standardization and measurement leads to a high error rate for sonographers. The automatic segmentation results of key structures in the hip joint can be used to develop a standard plane detection method that helps sonographers decrease the error rate. However, current automatic segmentation methods still face challenges in robustness and accuracy. Thus, we propose a neonatal hip bone segmentation network (NHBS-Net) for the first time for the segmentation of seven key structures. We design three improvements, an enhanced dual attention module, a two-class feature fusion module, and a coordinate convolution output head, to help segment different structures. Compared with current state-of-the-art networks, NHBS-Net gains outstanding performance accuracy and generalizability, as shown in the experiments. Additionally, image standardization is a common need in ultrasonography. The ability of segmentation-based standard plane detection is tested on a 50-image standard dataset. The experiments show that our method can help healthcare workers decrease their error rate from 6%-10% to 2%. In addition, the segmentation performance in another ultrasound dataset (fetal heart) demonstrates the ability of our network.
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http://dx.doi.org/10.1109/TMI.2021.3087857DOI Listing
June 2021

The complete chloroplast genome sequence of endangered plant (Arecaceae).

Mitochondrial DNA B Resour 2021 May 27;6(6):1772-1774. Epub 2021 May 27.

School of Ecology and Environmental Sciences Yunnan University, Kunming, Yunnan, China.

is an endangered plant that is endemic to southwest of China. In the present study, the complete chloroplast genome of this species was assembled and characterized using whole genome next-generation sequencing. The complete chloroplast genome showed a circular genome of 158,713 bp size with 36.6% GC content. The genome is of typical structure and contain a pair of inverted repeat (IR) regions with 27,240 bp, separated by one large single-copy (LSC) with 86,395 bp, and one small single-copy (SSC) regions with 17,838 bp. The genome contained 132 genes, including 86 protein-coding genes, 8 rRNA genes and 38 tRNA genes. A phylogenetic tree reconstructed based on 21 chloroplast genomes reveals that is most related with The information provides important genetic basis for the species' future studies on phylogenetic and utilization.
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http://dx.doi.org/10.1080/23802359.2021.1932625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168733PMC
May 2021

2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism.

Cell Discov 2021 Jun 8;7(1):43. Epub 2021 Jun 8.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China.

Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.
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http://dx.doi.org/10.1038/s41421-021-00273-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187413PMC
June 2021

OPG/TRAIL ratio as a predictive biomarker of mortality in patients with type A acute aortic dissection.

Nat Commun 2021 06 7;12(1):3401. Epub 2021 Jun 7.

Beijing Anzhen Hospital of Capital Medical University and Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China.

Following hospital discharge, patients with type A acute aortic dissection (TA-AAD) may present an increase in mortality risk. However, little is known about specific biomarkers associated with post-discharge survival, and there is a paucity of prognostic markers associated with TA-AAD. Here, we identify nine candidate proteins specific for patietns with TA-AAD in a cross-sectional dataset by unbiased protein screening and in-depth bioinformatic analyses. In addition, we explore their association with short-term and long-term mortality in a derivation cohort of patients with TA-AAD, including an internal (n = 300) and external (n = 236) dataset. An elevated osteoprotegerin (OPG)/tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) ratio was the strongest predictor of overall, 30-day, post-30-day mortality in both datasets and was confirmed to be a strong predictor of mortality in an independent validation cohort (n = 400). Based on OPG/TRAIL ratio-guided risk stratification, patients at high risk (>33) had a higher 1-year mortality (55.6% vs. 4.3%; 68.2% vs. 2.6%) than patients at low risk (<4) in both cohorts. In Conclusion, we show that an elevated OPG/TRAIL ratio is associated with a significant increase in short-term and long-term mortality in patients with TA-AAD.
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http://dx.doi.org/10.1038/s41467-021-23787-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185077PMC
June 2021

Rapid Method for Calculating the Conformationally Averaged Electronic Structure of Conjugated Polymers.

J Phys Chem B 2021 Jun 7;125(23):6338-6348. Epub 2021 Jun 7.

Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZD, UK.

We developed a rapid method to calculate the average electronic structure properties of large ensembles of conjugated polymer chains sampling their conformational space. This is achieved by using the localized molecular orbital (MO) method to rapidly compute the MOs and their energies for isolated polymer chains and through using a calibration scheme to further correct the obtained energies by comparison with a few accurate calculations. The method is applied to the study of the density of states and orbital localization characteristics for five polymers. It is shown that all key properties of the individual chain related to the charge mobility can be rationalized in terms of the properties of the constituent monomers, their interaction, and the conformational flexibility of the chain. More specifically we identify the features that lead to greater charge delocalization. Finally, we discuss the prospect of using this method for a computational high-throughput screening of conjugated polymers.
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http://dx.doi.org/10.1021/acs.jpcb.1c02866DOI Listing
June 2021

ZJ316 Attenuates -Induced Gastritis in C57BL/6 Mice.

J Agric Food Chem 2021 Jun 7;69(23):6510-6523. Epub 2021 Jun 7.

Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, China.

is a highly prevalent human-specific pathogen that causes various gastric diseases. In the present study, ZJ316, which could survive well in simulated gastrointestinal conditions, was found to have significant anti- ability. Animal assays revealed that ZJ316 had preventive and therapeutic effects on -induced gastritis. ZJ316 significantly decreased interferon γ (IFN-γ) and interleukin 6 (IL-6) levels, increased the IL-10 level, and repaired mucosal damage. Moreover, 16S rRNA gene sequencing revealed that the relative abundance of could be significantly reduced by ZJ316 administration. Members of the families Dehalobacteriaceae and Geodermatophilaceae were more prevalent in the prevention group, while Lactobacillaceae and Actinomycetaceae were more prevalent in the treatment group. These results indicate that ZJ316 serves as a potential candidate for the prevention and treatment of -induced gastritis by regulating the gastric microbiota and reducing mucosal inflammation.
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http://dx.doi.org/10.1021/acs.jafc.1c01070DOI Listing
June 2021

Integrating Spatial Transcriptomics and Single-Cell RNA-seq Reveals the Gene Expression Profling of the Human Embryonic Liver.

Front Cell Dev Biol 2021 20;9:652408. Epub 2021 May 20.

Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, China.

The liver is one of vital organs of the human body, and it plays an important role in the metabolism and detoxification. Moreover, fetal liver is one of the hematopoietic places during ontogeny. Understanding how this complex organ develops during embryogenesis will yield insights into how functional liver replacement tissue can be engineered and how liver regeneration can be promoted. Here, we combine the advantages of single-cell RNA sequencing and Spatial Transcriptomics (ST) technology for unbiased analysis of fetal livers over developmental time from 8 post-conception weeks (PCW) and 17 PCW in humans. We systematically identified nine cell types, and defined the developmental pathways of the major cell types. The results showed that human fetal livers experienced blood rapid growth and immigration during the period studied in our experiments, and identified the differentially expressed genes, and metabolic changes in the developmental process of erythroid cells. In addition, we focus on the expression of liver disease related genes, and found that 17 genes published and linked to liver disease mainly expressed in megakaryocyte and endothelial, hardly expressed in any other cell types. Together, our findings provide a comprehensive and clear understanding of the differentiation processes of all main cell types in the human fetal livers, which may provide reference data and information for liver disease treatment and liver regeneration.
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http://dx.doi.org/10.3389/fcell.2021.652408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173368PMC
May 2021

Perioperative Exercise Intention and Influencing Factors: A Multi-Centered Cross-Sectional Study.

Front Public Health 2021 20;9:653055. Epub 2021 May 20.

Department of Anesthesiology, Youyang Hospital, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

This study aimed to evaluate the level and factors affecting the perioperative exercise intention in China. This study was a cross-sectional survey in Southwest China. Four hundred and ninety nine participants were randomly sampled in eight medical centers from November 23, 2020 to November 27, 2020. The survey included sociodemographic information and a 24-item modified questionnaire, which aimed to evaluate the attitude toward daily exercise, perception of perioperative exercise, social support and the perioperative exercise intention. A multivariable linear regression model was used to evaluate the effect of different items on the patients' intention for perioperative exercise. A total of 523 responses (95.09%) were collected and 499 (95.41%) were analyzed. The level of exercise intention of the patients during the perioperative period was: 14.83% planned to exercise every day in the hospital, 21.04% planned to exercise every other day, and 35.87% planned to exercise every week. Intensity of daily exercise ( = 0.016), positive attitude of daily exercise ( < 0.001), positive attitude of perioperative exercise ( < 0.001) and social support ( < 0.001) were positively associated with the intention for perioperative exercise. Female ( = 0.012), non-tertiary center ( = 0.011), and preoperative anxiety ( = 0.023) was negatively associated with it. The intention for perioperative exercise was low in Southwest China. The authors aimed to relieve preoperative anxiety, promote the education of perioperative exercise, design perioperative exercise programs, and provide more social support from medical staff and family for inpatients undergoing elective surgery.
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http://dx.doi.org/10.3389/fpubh.2021.653055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172588PMC
June 2021

Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer.

Am J Cancer Res 2021 15;11(5):2005-2024. Epub 2021 May 15.

Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center Houston, Texas, USA.

Prevention of estrogen receptor-negative (ER-) breast cancer is an unmet challenge, although tamoxifen and aromatase inhibitors can successfully decrease the incidence of ER-positive (ER+) breast cancer. PI3K pathway activation has been detected in tamoxifen-resistant ER- breast lesions of patients. Here, we further ratified that the PI3K pathway is significantly activated in premalignant ER- breast lesions compared with paired normal tissues of patients, which prompted our assessment of targeting PI3K on inhibition of ER- mammary tumor initiation and progression. Both genetic knockdown of PIK3CA or intervention with low-doses of a PI3K inhibitor (GDC-0941) prevented the dysplasia phenotype of semi-transformed human ER- mammary epithelial cells in 3-dimensional culture . Importantly, low-dose GDC-0941 treatment significantly delayed mammary tumor initiation in the MMTV- mouse model without exhibiting discernable adverse effects. Interestingly, increased CD8/GZMB T-cells were detected in mammary tissue after GDC-0941 treatment, suggesting enhanced immune surveillance. Mechanistically, elevated expression of potent T-cell chemo-attractants, including CCL5 and CXCL10, were detected both and after GDC-0941 treatment. Furthermore, inhibition of PI3K significantly increased T-cell recruitment in a CCL5/CXCL10-dependent manner. In human ER- breast cancer, PI3K activation is correlated with significantly reduced and expression, suggesting that the decreased CD8 T-cell recruitment and escape of immune surveillance may contribute to ER- breast cancer development. In summary, our study indicates that low-dose PI3K inhibitor treatment may intervene early stage ER- breast cancer development by enhancing immune surveillance via CCL5/CXCL10.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167687PMC
May 2021

visualization of peroxisomal viscosity in the liver of mice with non-alcoholic fatty liver disease by near-infrared fluorescence and photoacoustic imaging.

Chem Sci 2020 Oct 6;11(44):12149-12156. Epub 2020 Oct 6.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University Jinan 250014 People's Republic of China

Non-alcoholic fatty liver disease (NAFLD) can gradually develop into hepatic failure, and early diagnosis is crucial to improve treatment efficiency. The occurrence of NAFLD is closely related to lipid metabolism. Peroxisomes act as the first and main site for lipid metabolism in the hepatocytes, so abnormal lipid metabolism might directly affect peroxisomal viscosity. Herein, we developed a new near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging probe (PV-1) for the real-time visualization of peroxisomal viscosity . This PV-1 encompasses the malononitrile group as the rotor, which emits strong NIRF (at 705 nm) and PA (at 680 nm) signals when rotation is hindered as viscosity increases. Through dual-mode imaging, we discovered distinctly higher viscosity in the liver of NAFLD mice for the first time. We further found the remarkable amelioration of NAFLD upon treatment with -acetylcysteine (NAC). Therefore, we anticipate that the PV-1 imaging method is promising for the early diagnosis and prognostic evaluation of NAFLD.
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http://dx.doi.org/10.1039/d0sc02922jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163019PMC
October 2020

Construction of direct Z-scheme photocatalyst by the interfacial interaction of WO and SiC to enhance the redox activity of electrons and holes.

Chemosphere 2021 May 25;282:130866. Epub 2021 May 25.

College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 401331, China; College of Physics, Chongqing University, Chongqing, 401331, China. Electronic address:

The direct Z-scheme heterojunction structure benefits separation and migration of photoinduced carriers while maintaining original redox ability of each component. Nowadays, most Z-scheme structures are fabricated by g-CN with other narrow band photocatalysts due to its low conduction band (CB). In this paper, SiC, another kind of photoelectric semiconductor with low CB, was employed to prepare direct Z-scheme photocatalyst with 2D WO by simple water oxidation precipitation method. The component and interface band structure of Z-scheme heterojunction WO/SiC (WS) were verified by XPS, KPFM, Mott-Schottky method. The photodegradation efficiency and rate constant values of WS-1 for degrading RhB enhanced 2.5 and 5.3 times respectively compared with pristine WO. Radical capture experiments and ESR tests affirmed that WS-1 photocatalyst produced •OH and •Oactive species, which further confirmed the photogenerated carriers were transmitted through the Z-scheme mode in principle. Band structure investigation showed that the direct Z-scheme structure assembled by WO with high valence band (VB) and SiC with low CB could maintain the high photocatalytic activity of active species. Therefore, this study offers a feasible method for construction of a novel and efficient direct Z-scheme photocatalyst.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130866DOI Listing
May 2021

An insight into the exploration of proliferation of antibiotic resistance genes in high-fat diet induced obesity mice.

Genomics 2021 Jun 2;113(4):2503-2512. Epub 2021 Jun 2.

Laboratory of Nutrition and Development,Beijing Pediatric Research Institute,Key Laboratory of Major Diseases in Children,Ministry of Education,Beijing Children's Hospital,Capital Medical University,National Center for Children's Health, Beijing 100045, PR China. Electronic address:

Using mice as an animal model, we first demonstrated the significant proliferation of ARGs and the change of mobile genetic elements (MGEs) in high-fat diet induced obesity (DIO) mice, which the ermB and tnpA-03 genes mostly increased, illuminating that DIO could enrich the abundance of ARGs. Additionally, Lactobacillus sharply increased in the DIO mice and might contribute to the proliferation of ARGs and dramatical change of MGEs in the HFD groups. Finally, procrustes analysis showed the explanatory variables of the MGEs, the metabolites, and the microbial communities for the ARGs accounted for 94.3%, 53.4%, and 68.1%, respectively, and implying that MGEs might be the most direct factor affecting ARGs, and microbiota could be the main driver of the proliferation of ARGs in the DIO mice.
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http://dx.doi.org/10.1016/j.ygeno.2021.05.041DOI Listing
June 2021

Metabolic cold adaptation in the Asiatic toad: intraspecific comparison along an altitudinal gradient.

J Comp Physiol B 2021 Jun 5. Epub 2021 Jun 5.

CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

The metabolic cold adaptation (MCA) hypothesis predicts an increase in metabolic rate and thermal sensitivity of poikilotherms from cold environments as compared to those from warm environments, when measured under standardized conditions. This compensatory response is also expected to evolve in life history and behavioral traits if the reductions in these phenotypic traits at low temperature involves in a reduction in fitness. We investigated the extent to which the level of energy intake (measured as feeding rate), energy turnover (measured as standard metabolic rate, SMR) and the energy budget (energy allocation to growth and physical activity) are influenced by climatic conditions in three populations of the Asiatic toad (Bufo gargarizans) distributed across an altitudinal gradient of 1350 m in the Qionglai Mountains of Western China. We found a similar thermal reaction norm of SMR at both population and individual levels; therefore, the data did not support the MCA hypothesis. However, there was a co-gradient variation (CoGV) for mass change rate in which the high and medium altitudinal populations displayed slower mass change rates than their counterparts from low altitudes. Moreover, this CoGV pattern was accompanied by a low feeding rate and high physical activity for the high- and medium-altitude populations. Our results highlight that adjustments in energy intake and energy allocation to behaviors, but not energy allocation to metabolism of maintenance, could act as an energetic strategy to accommodate the varied growth efficiency in Asiatic toads along an altitudinal gradient.
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http://dx.doi.org/10.1007/s00360-021-01381-xDOI Listing
June 2021

Prognostic analysis of patients with intra-abdominal infectious complications after laparoscopic-assisted and open radical gastrectomy for gastric cancer - A propensity score-matching analysis.

Surg Oncol 2021 Jun 14;37:101583. Epub 2021 May 14.

Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, China; Department of General Surgery, Fujian Medical University Union Hospital, Fuzhou, China; Key Laboratory of Ministry of Education of Gastrointestinal Cancer, Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Tumor Microbiology, Fujian Medical University, Fuzhou, China. Electronic address:

Background: To investigate the incidence and prognosis of intra-abdominal infectious complications (IaICs) after laparoscopic-assisted gastrectomy (LAG) and open radical gastrectomy (OG) for gastric cancer.

Methods: The data of patients who underwent radical gastrectomy (LAG and OG) for gastric cancer at the Fujian Medical University Union Hospital from January 2000 to December 2014 were retrospectively reviewed. A 1:1 propensity score matching (PSM) was used to reduce bias. The incidence and prognosis of postoperative IaICs in the two groups were analyzed.

Results: After PSM, no significant difference was found in the baseline data between OG (n = 913) and LAG (n = 913). The incidence of IaICs after OG and LAG was 4.1% and 5.1%, respectively (p = 0.264). The Cox multivariate analysis showed that IaICs were an independent risk factor for overall survival (OS) of patients undergoing gastrectomy (hazard ratio [HR]: 1.65, p < 0.001). Further, LAG was an independent protective factor for OS among the patients with IaICs (HR: 0.54, p = 0.036), while tumor diameter of ≥50 mm (p = 0.01) and pathological TNM stage III (p < 0.001) were independent risk factors. The 5-year OS rate was higher in the patients with IaICs who underwent LAG than in those who underwent OG (51.1% vs. 32.4%, p = 0.042). The prognostic nutritional index was similar in both groups before surgery (p = 0.220) but lower on the first, third, and fifth days after OG than after LAG (p < 0.05).

Conclusions: Compared to OG, LAG can improve the prognosis of patients with postoperative IaICs and is therefore recommended for patients at a high risk for IaICs.
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http://dx.doi.org/10.1016/j.suronc.2021.101583DOI Listing
June 2021

Effect of silane coupling agent on compatibility interface and properties of wheat straw/polylactic acid composites.

Int J Biol Macromol 2021 Jun 1;182:2108-2116. Epub 2021 Jun 1.

College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, PR China. Electronic address:

To improve the performance of wheat straw/polylactic acid (WS/PLA) composites, four different silane coupling agents were used for constructing compatible interfaces and then examined by scanning electron microscopy, Fourier transform-infrared spectroscopy, X-ray diffractometry and thermogravimetric analysis. The blending and tensile strengths of silane-modified composites were effectively enhanced, with KH-570-modified composite exhibiting the best blending and tensile strengths. Water resistance analysis of silane-modified composites was reduced and contact angles larger, indicating that water resistance performance of this composite had been effectively improved. The KH-570-modified composite exhibited the best water resistance performance. Strain scanning showed that, in the linear viscoelastic region, the storage modulus (G') of modified composite was larger than that of unmodified composites. Frequency scanning showed that the G' and complex viscosity (η*) of modified composites were greater than those of unmodified composites. From strain analysis and frequency scanning, the modified performance of the silane agent was observed to effectively improve composite interfacial compatibility, with KH-570-modified composite exhibiting the best effect. XRD analysis showed that silane coupling agent modification improved the crystallinity of composites with the improvement of KH-570 the best. And the thermal stability of silane-modified composites was improved and the thermal stability of KH-570-modified composite the best.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.207DOI Listing
June 2021