Publications by authors named "Ping Chen"

2,628 Publications

  • Page 1 of 1

Morphological Variations of the Posterior Superior Iliac Spine in Chinese Population: Potential Effects on the Reliability of Palpation.

Biomed Res Int 2022 8;2022:6290133. Epub 2022 Aug 8.

Department of Orthopaedics, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.

Objectives: The posterior superior iliac spine (PSIS) is an important anatomical landmark often involved in spinal manipulation and surgical bone harvest. Hence, knowledge of variations in the PSIS may be predictive and valuable in clinical settings. Taking the complex morphology into account, the study is aimed at proposing a classification of PSIS in the Chinese population.

Methods: An anatomical study was undertaken on 288 human ilia. First, the morphological features of variations in the PSIS were noted following visual inspection. Then, 12 variable anatomical parameters were measured in order to determine the differences based on morphology, side, and sex.

Results: Overall, four types of PSIS were found among 288 bones, including type I "V-shape" (106, 36.8%), type II "U-shape" (121, 42.0%), type III "W-shape" (36, 12.5%), and type IV "ossification-shape" (25, 8.7%). There were no significant sex or bilateral differences in the morphological distribution of the PSIS ( > 0.05). Furthermore, the measurements showed that type I was the narrowest and type III the broadest ( < 0.05). Moreover, female specimens had an overall larger distance and width of surrounding landmarks ( < 0.05), and a significant difference was found in the width of the PSIS between the left and right sides ( < 0.05).

Conclusion: The PSIS samples displayed multiple morphological variations and could be classified into four types. In addition, sex-based or bilateral differences existed in the size and relative positions. It is thus likely that differences in the morphology and asymmetry of the PSIS provide references for palpation, bone harvest, and other clinical settings.
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http://dx.doi.org/10.1155/2022/6290133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377927PMC
August 2022

EGR2 is a hub-gene in myocardial infarction and aggravates inflammation and apoptosis in hypoxia-induced cardiomyocytes.

BMC Cardiovasc Disord 2022 Aug 15;22(1):373. Epub 2022 Aug 15.

Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Chongqing Medical University, #76 Linjiang Road, Yuzhong District, Chongqing, 400010, China.

Background: Myocardial infarction (MI) is characterized by coronary artery occlusion, ischemia and hypoxia of myocardial cells, leading to irreversible myocardial damage. Therefore, it is urgent to explore the potential mechanism of myocardial injury during the MI process to develop effective therapies for myocardial cell rescue.

Methods: We downloaded the GSE71906 dataset from GEO DataSets, and the R software was used to identify the differentially expressed genes (DEGs) in mouse heart tissues of MI and sham controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed to understand the significantly activated signaling pathways in MI. Protein-protein interaction (PPI) network was constructed to highlight the hub genes in DEGs. The Western Blot, qRT-PCR and TUNEL staining were used to explore the function of hub gene in hypoxia-induced cardiomyocytes in vitro.

Results: A total of 235 DEGs were identified in GSE71906 dataset. Functional enrichment analysis revealed that the upregulated genes were primarily associated with the inflammatory response and apoptosis. 20 hub genes were identified in PPI network, and the early growth response 2 (EGR2) was highlighted. In vitro. We confirmed the EGR2 was upregulated induced by hypoxia and revealed the upregulated EGR2 aggravates pro-inflammation and pro-apoptotic genes expression. In addition, EGR2 knockout mitigates hypoxia-induced inflammation and apoptosis in cardiomyocytes.

Conclusion: The present study identified the EGR2 was a hub gene in myocardial damage during MI process, the excessive EGR2 aggravates hypoxia-induced myocardial damage by accelerating inflammation and apoptosis in vitro. Therefore, targeting EGR2 offers a potential pharmacological strategy for myocardial cell rescue in MI.
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http://dx.doi.org/10.1186/s12872-022-02814-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377070PMC
August 2022

A clinical strain with carried by an integrative and conjugative element in China.

Front Microbiol 2022 29;13:929800. Epub 2022 Jul 29.

Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China.

Objective: To precisely determine the species of a carbapenem-resistant strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) formation mechanism.

Methods: Single-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE were aligned. At the same time, the transfer ability of and the antibiotic sensitivity of 18091276 were tested.

Results: This bacterium was , and its drug resistance mechanism is the capture of the gene cassette by the Tn-like type 1 integron (- ) to form In before its capture by the ΔTn-carrying ICE . The acquisition of confers carbapenem resistance to 18091276.

Conclusion: The formation of -carrying ICE , its further integration into the chromosomes, and transposition and recombination of other elements promote bacterial gene accumulation and transmission.
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http://dx.doi.org/10.3389/fmicb.2022.929800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374279PMC
July 2022

The Prediction of Enterprise Stock Change Trend by Deep Neural Network Model.

Comput Intell Neurosci 2022 2;2022:9193055. Epub 2022 Aug 2.

International College, Krirk University, Maha Nakhon, Bangkok 10220, Thailand.

This study aims to accurately predict the changing trend of stocks in stock trading so that company investors can obtain higher returns. In building a financial forecasting model, historical data and learned parameters are used to predict future stock prices. Firstly, the relevant theories of stock forecasting are discussed, and problems in stock forecasting are raised. Secondly, the inadequacies of deep neural network (DNN) models are discussed. A prediction trend model of enterprise stock is established based on long short-term memory (LSTM). The uniqueness and innovation lie in using the stock returns of Bank of China securities in 2022 as the training data set. LSTM prediction models are used to perform error analysis on company data training. The 20-day change trend of the company's stock returns under different models is predicted and analyzed. The results show that as the number of iterations increases, the loss rate of the LSTM training curve keeps decreasing until 0. The average return price of the LSTM prediction model is 14.01. This figure is closest to the average real return price of 13.89. Through the forecast trend analysis under different models, LSTM predicts that the stock change trend of the enterprise model is closest to the changing trend of the actual earnings price. The prediction accuracy is better than other prediction models. In addition, this study explores the characteristics of high noise and complexity of corporate stock time series, designs a DNN prediction model, and verifies the feasibility of the LSTM model to predict corporate stock changes with high accuracy.
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http://dx.doi.org/10.1155/2022/9193055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363192PMC
August 2022

Improving Output Efficiency of InGaN-Based MQW Green Laser Diodes by Modulating Indium Content of Quantum Barriers and Using Composite Lower Waveguide Layers.

Nanomaterials (Basel) 2022 Jul 27;12(15). Epub 2022 Jul 27.

State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Potential barriers between the waveguide layer and MQW active region may influence injection efficiency significantly, which is important in improving output characteristics of GaN-based green laser diodes (LDs). In this study, potential barriers and injection efficiency of LDs are investigated by simulation methods. It is found that different indium content in quantum barrier layers results in different potential barrier heights, leading to different recombination rates in upper and lower waveguide layers, and the injection efficiency can be modulated effectively. An eclectic choice of indium content can suppress recombination in two waveguide layers, improving the output characteristics of green LDs. Additionally, a composite lower waveguide layer structure is proposed to reduce the negative effect of potential barriers. High output power and low threshold current are achieved owing to the reduction in electron injection blockage and hole leakage effects.
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http://dx.doi.org/10.3390/nano12152581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370496PMC
July 2022

Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from (Hook.f.) Woodson.

Plants (Basel) 2022 Jul 28;11(15). Epub 2022 Jul 28.

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.

a traditional medicinal plant used primarily as tea. It has a potential health benefit from its rich bioactive substances. This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the leaf. The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC). The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides. Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW). It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively. Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract. The principal component analysis revealed a positive correlation between the constituents, bioactivities, and extracts. The overall result showed as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
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http://dx.doi.org/10.3390/plants11151964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370764PMC
July 2022

Introduction to the Special Issue.

Hemoglobin 2022 Jan;46(1)

Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

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http://dx.doi.org/10.1080/03630269.2021.2008955DOI Listing
January 2022

THALASSEMIA in ASIA 2021: Thalassemia in Guangxi Province, People's Republic of China.

Hemoglobin 2022 Jan;46(1):33-35

Key Laboratory of Thalassemia Research, Thalassemia Research Institute, Key Laboratory of Thalassemia Medicine, Chinese Academy of Medical Sciences, First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.

Guangxi Province is located in the southwest of the People's Republic of China (PRC). The province has a population of 50.12 million with a birth rate of 13.31%. Thalassemia is a major health problem in Guangxi Province. About 20.0-25.0% of the population carries thalassemia genes, which is acknowledged to be the highest prevalence in China. National and provincial programs for thalassemia prevention and control have been introduced. Premarital screening and prenatal diagnosis (PND) for the prevention of thalassemic fetuses are available. Blood transfusions, iron chelation therapy, and stem cell transplantation are also available for transfusion-dependent thalassemic patients.
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http://dx.doi.org/10.1080/03630269.2021.2008960DOI Listing
January 2022

Acute cardiorespiratory response to air quality index in healthy young adults.

Environ Res 2022 Aug 7;214(Pt 3):113983. Epub 2022 Aug 7.

Department of Toxicology, School of Public Health, Anhui Medical University, Hefei, 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Anhui Medical University, Hefei, 230032, China. Electronic address:

Background: Little is known about the acute health impacts of air quality index (AQI) on cardiorespiratory risk factors.

Objectives: To assess the short-term links of AQI with cardiorespiratory risk factors in young healthy adults.

Methods: We performed a longitudinal panel study with 4 repeated visits in 40 healthy young adults in Hefei, Anhui Province, China from August to October 2021. Cardiorespiratory factors included systolic blood pressure (BP), diastolic BP (DBP), mean arterial pressure (MAP) and fractional exhaled nitric oxide (FeNO). We collected hourly AQI data from a nearby air quality monitoring site. Linear mixed-effects model was applied to assess the effects of AQI on BP and FeNO.

Results: The study participants (75.0% females) provided 160 pairs of valid health measurements with average age of 24 years. The mean AQI level was 44.43 during the study period. There were significant positive associations of AQI with three BP parameters and FeNO at different lag periods. For example, an interquartile range increase in AQI (26.54 unit) over lag 0-24 h was associated with increments of 6.69 mmHg (95%CI: 2.95-10.44), 5.71 mmHg (95%CI: 3.30-8.13), 6.04 mmHg (95%CI: 3.46-8.62) and 5.67% (95%CI: 1.05%-16.05%) in SBP, DBP, MAP and FeNO, respectively. The results were robust after controlling for PM. We did not find effect modifications by gender, BMI, physical activity, or AQI category level on the associations.

Conclusions: The current findings on associations of AQI with cardiorespiratory factors might add evidence of the acute adverse cardiorespiratory consequences following air pollution.
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http://dx.doi.org/10.1016/j.envres.2022.113983DOI Listing
August 2022

Rapid titration with oral sustained-release morphine plus subcutaneous morphine in a multi-center, randomized control study of cancer patients with moderate to severe cancer pain.

Jpn J Clin Oncol 2022 Aug 10. Epub 2022 Aug 10.

Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, China.

Background: Pain is one of the most common concomitant symptoms among cancer patients. Pharmacologic agents are regarded as a cornerstone of cancer pain management. 'Dose titration' with short-acting morphine is widely accepted. Such a titration method is very complicated. The analgesic background establishment is often delayed. Titration based on sustained-release opioids is also recommended, but the onset of analgesic effect requires hours, whereas the rescue analgesia is always needed. This study evaluated the optimized morphine titration scheme with a simultaneous combination of sustained-release morphine and subcutaneous morphine.

Methods: In a multicenter, 7-day, randomized controlled study, patients with moderate to severe cancer pain were assigned to receive either sustained-release morphine and subcutaneous morphine simultaneously (rapid titration) or only subcutaneous morphine to dose titration. The primary outcome was the safety and the number of times of rescue therapy as needed in the first 24 h.

Results: A total of 108 patients with moderate to severe cancer pain were included in the study. The number of times of rescue analgesics in the first 24 h significantly reduced in the rapid titration group (0.4 ± 0.48 vs. 2.3 ± 0.78, P = 0.000). No differences in the intensity of opioid-related symptoms were found between the two groups.

Conclusions: Rapid titration is safe and efficient, which could significantly decrease rescue analgesics in the first 24 h and achieve better analgesic efficacy for cancer pain patients.
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http://dx.doi.org/10.1093/jjco/hyac128DOI Listing
August 2022

Effectiveness of 2 Pretreatment Methods in Antifogging of Goggles in a COVID-19 Isolation Ward: A Randomized Controlled Trial.

J Emerg Nurs 2022 Jun 28. Epub 2022 Jun 28.

Introduction: This study aimed to compare the effectiveness of the pretreatment of goggles with iodophor solution and antibacterial hand sanitizer to reduce the fogging of goggles.

Methods: A total of 90 health care workers were divided into a control group (n = 30), an iodophor solution group (n = 30), and an antibacterial hand sanitizer group (n = 30). This study evaluated the degree of fogging of goggles and the light transmission, comfort, eye irritation, and the impact of goggles on the medical work of staff.

Results: The antibacterial hand sanitizer group had the lowest amount of goggle fogging and the most transparent view. Participants in the control group reported the worst light transmission and comfort level, followed by the iodophor solution group. In contrast, the goggles in the antibacterial hand sanitizer group had the best light transmission and comfort level. The iodophor solution group participants reported more eye irritation. Participants in the control group reported that the goggles severely impacted their medical work, with a less severe impact reported by the iodophor solution group. The antibacterial hand sanitizer group did not report any impact on their medical work.

Conclusion: When the goggles were internally coated with antibacterial hand sanitizer solution (diluted 1:1 with distilled water), the antifog effect was significant. Moreover, the goggles treated with antibacterial hand sanitizer had a clearer field of vision, were reported as non-irritating to the eyes, and significantly improved the efficiency of COVID-19 health care workers, including emergency nurses and providers.
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http://dx.doi.org/10.1016/j.jen.2022.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237021PMC
June 2022

dGLYAT modulates Gadd45-mediated JNK activation and cell invasion.

Cell Div 2022 Aug 6;17(1). Epub 2022 Aug 6.

The First Rehabilitation Hospital of Shanghai, Shanghai Key Laboratory of Signaling and Diseases Research, School of Life Science and Technology, Tongji University, Shanghai, China.

Background: Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion.

Results: We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and cell invasion. Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. Finally, Gadd45 knockdown suppressed scrib depletion-induced JNK pathway activation and cell invasion.

Conclusions: These evidences reveal the role of dGLYAT and Gadd45 in JNK-dependent cell invasion, and provide insight for the roles of their human homologs in cancers.
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http://dx.doi.org/10.1186/s13008-022-00080-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357319PMC
August 2022

Transient Inhibition of Trastuzumab-Tumor Binding to Overcome the "Binding-Site Barrier" and Improve the Efficacy of a Trastuzumab-Gelonin Immunotoxin.

Mol Cancer Ther 2022 Aug 5. Epub 2022 Aug 5.

University at Buffalo, State University of New York, Buffalo, New York, United States.

We have recently shown that co-administration of monoclonal antibodies (mAb) with anti-idiotypic distribution enhancers (AIDEs) that inhibit mAb binding to tumor antigens enabled increased intra-tumoral mAb distribution and increased efficacy of an antibody-drug conjugate (trastuzumab emtansine, T-DM1). In the present work, a PK/PD model was applied to predict the impact of this optimization strategy on the within-tumor distribution and anti-tumor efficacy of trastuzumab-gelonin, where the released payload (gelonin) is expected to exhibit negligible bystander activity. Immunofluorescence histology was used to investigate trastuzumab-gelonin distribution in solid tumors following dosing with or without co-administration of anti-trastuzumab AIDEs. Anti-tumor efficacy of trastuzumab-gelonin, with or without co-administration of AIDEs, was also evaluated in tumor-bearing mice. Trastuzumab-gelonin efficiently induced cytotoxicity when applied to NCI-N87 cells in culture (IC50: 0.224±0.079 nM). PK/PD simulations predicted that anti-idiotypic single-domain antibodies AIDEs with dissociation rate constants between 0.03-0.2 hour-1 would provide optimal enhancement of trastuzumab-gelonin efficacy. LE8 and 1HE, anti-trastuzumab AIDEs, were selected for evaluation in vivo. Co-administration of trastuzumab-gelonin with the inhibitors increased the portion of tumor area that stained positive for trastuzumab-gelonin by 58% (p=0.0059). Additionally, LE8 or 1HE co-administration improved trastuzumab-gelonin efficacy in NCI-N87 xenograft bearing mice by increasing the percent increase in life span (%ILS) from 27.8% (for trastuzumab-gelonin administered alone) to 62.5% when administered with LE8 (p=0.0007) or 83.3% (p=0.0007) when administered with 1HE. These findings support the hypothesis that transient, competitive inhibition of mAb-tumor binding can improve the intra-tumoral distribution and efficacy of immunotoxins when applied for treatment of solid tumors.
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http://dx.doi.org/10.1158/1535-7163.MCT-22-0192DOI Listing
August 2022

PVR/TIGIT and PD-L1/PD-1 expression predicts survival and enlightens combined immunotherapy in lung squamous cell carcinoma.

Transl Oncol 2022 Oct 1;24:101501. Epub 2022 Aug 1.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 17 Panjiayuannanli, Beijing, China. Electronic address:

PVR/TIGIT and PD-L1/PD-1 axes play essential roles in tumor immune evasion and could be potential targets for combined immunotherapy. We aimed to evaluate the expression status of the above-mentioned immune markers in lung squamous cell carcinoma (LUSC), and investigate their survival impact and relevance with the immune microenvironment and clinicopathological features. We retrospectively collected specimens from 190 LUSC patients, who underwent pulmonary surgeries, and we performed immunohistochemistry assays of PVR, TIGIT, PD-L1, PD-1 and CD8. In our cohort, the positive rate of PVR was 85.8%, which was much higher than the positive rate of PD-L1 at 26.8%. A total of 32 (16.8%) patients demonstrated co-expression of PVR/PD-L1. High TIGIT density was correlated with positive PD-L1 expression, high PD-1 density, and high CD8 density (PD-L1, P=0.033; PD-1, P<0.001; CD8, P<0.001), and positive PVR expression was correlated with positive PD-L1 expression (P=0.046). High TIGIT density and high PVR/TIGIT expression were correlated with advanced TNM stage (TIGIT density, P=0.020; PVR/TIGIT expression, P=0.041). Patients with positive PVR expression, high TIGIT density, high PVR/TIGIT expression and PVR/PD-L1 co-expression exhibited a significantly worse prognosis (PVR, P=0.038; TIGIT, P=0.027; PVR/TIGIT, P=0.014; PVR/PD-L1, P=0.018). Multivariate analysis demonstrated that PVR/PD-L1 co-expression (Hazard ratio [HR], 1.756, 95% CI, 1.152-2.676, P=0.009) was an independent prognostic factor in LUSC patients. In conclusion, we demonstrated the expression status of PVR/TIGIT and PD-L1/PD-1 in LUSC. PVR/PD-L1 co-expression was an independent prognostic factor in LUSC patients and may serve as a potential predictive biomarker for dual-targeting immunotherapy.
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http://dx.doi.org/10.1016/j.tranon.2022.101501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352965PMC
October 2022

Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers.

Front Bioeng Biotechnol 2022 18;10:909388. Epub 2022 Jul 18.

Department of Thoracic Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.

Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher's exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related () and tet methylcytosine dioxygenase 2 () genes were significantly enriched in lymphatic metastases ( ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated and might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes.
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http://dx.doi.org/10.3389/fbioe.2022.909388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341247PMC
July 2022

Transition-Metal-Free Barium Hydride Mediates Dinitrogen Fixation and Ammonia Synthesis.

Angew Chem Int Ed Engl 2022 Aug 2:e202205805. Epub 2022 Aug 2.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Transition-metal-mediated dinitrogen fixation has been intensively investigated. The employment of main group elements for this vital reaction has recently sparked interest because of new dinitrogen reaction chemistry. We report ammonia synthesis via a chemical looping process mediated by a transition-metal-free barium hydride (BaH ). Experimental and computational studies reveal that the introduction of hydrogen vacancies is essential for creating multiple coordinatively unsaturated Ba sites for N activation. The adjacent lattice hydridic hydrogen (H ) then undergoes both reductive elimination and reductive protonation to convert N to NH . The ammonia production rate supports this hydride-vacancy mechanism via a chemical looping route that far exceeds that of a catalytic process. The BaH -mediated chemical looping process has prospects in future technologies for ammonia synthesis using transition-metal-free materials.
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http://dx.doi.org/10.1002/anie.202205805DOI Listing
August 2022

Identification of Key Headspace Volatile Compounds Signaling Preference for Rice over Corn in Adult Females of the Rice Leaf Folder .

J Agric Food Chem 2022 Aug 2;70(32):9826-9833. Epub 2022 Aug 2.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Volatile organic compounds are important for herbivorous insects in locating their host plants. The rice leaf folder, (Guenée), is a devastating migratory insect pest of rice in Asian countries. Although can develop even better on corn than on rice plants in insectaries, it rarely occurs on corn plants in the field. We hypothesized that plant volatile-mediated oviposition preference for rice over corn in adult females may be the reason for the observed rare field occurrence of the pest on corn plants. The present study was conducted to identify the olfactory active volatile compounds (OAVCs) that enable females to discriminate rice from corn plants. In cage tests, rice plants were highly preferred for oviposition over corn plants by females. From headspace, chemical analyses identified 15 rice unique, 8 corn unique, and 28 common volatile compounds. Fourteen OAVCs, including seven common, five rice unique, and two corn unique, were determined. In electroantennogram tests, the rice unique and common OAVCs activated the antennal responses in . In Y-tube olfactometer tests, ()-2-hexenal and 3-hexanol(common OAVCs) and ()-3-hexenyl acetate and ()-2-hexen-1-ol (rice unique OAVCs) attracted more females than the control, and only blends with both rice unique and common OAVCs were highly preferred over the control. Our results provide insights into the chemical cues used by adult females in host location, which may aid the development of novel crop protection strategies based on the manipulation of host-finding behaviors of .
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http://dx.doi.org/10.1021/acs.jafc.2c01948DOI Listing
August 2022

Highly Effective Markerless Genetic Manipulation of Using a Mutated PheS-Based Counterselectable Marker.

Front Microbiol 2022 14;13:947821. Epub 2022 Jul 14.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

is an important zoonotic pathogen, however, an efficient markerless genetic manipulation system is still lacking for further physiological and pathological studies on this bacterium. Several techniques have been developed for markerless genetic manipulation of utilizing either a temperature-sensitive vector or a counterselectable markers (CSMs), however, at present, the efficiency of these techniques is not very satisfactory. In this study, we developed a strategy for markerless genetic manipulation of employing a CSM based on a conditionally lethal mutant allele of , which encodes the α-subunit of phenylalanyl-tRNA synthetase (PheS). This mutant , , was constructed by introducing site-directed mutations for a T261S/A315G double-substitution and a number of silent mutations to decrease its similarity with the endogenous wild type gene (). Additionally, five potentially strong promoters from were screened for their ability to drive high-level expression of mPheS, thus endowing the carrier strain with sufficient sensitivity to the phenylalanine analog -chloro-phenylalanine (-Cl-phe). Insertion of these P- cassettes into a vector or into the chromosomal locus a linked erythromycin resistance gene revealed that allele driven by promoters P and P renders sensitive to as low as 0.01% (or 0.5 mM) of -Cl-phe. This offers two potential CSMs for with -Cl-phe as a counterselective agent. P- was revealed to be 100% efficient for counter-selection in by application in a precise gene deletion. Using P- as a CSM, a two-step insertion and excision strategy for markerless genetic manipulation of were developed, supplying a powerful tool for markerless genetic manipulation of .
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http://dx.doi.org/10.3389/fmicb.2022.947821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329067PMC
July 2022

Serum Serine and the Risk of All-Cause Mortality: A Nested Case-Control Study From the China Stroke Primary Prevention Trial (CSPPT).

Front Nutr 2022 12;9:946277. Epub 2022 Jul 12.

Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health, College of Food Sciences and Nutritional Engineering, China Agricultural University, Beijing, China.

Background: Serine plays a key role in numerous cellular processes, the levels and metabolism is therefore of critical importance. However, few data are available to illustrate the association of serine with long-term health effects, especially, the predictive value for long-term mortality.

Objective: This study was conducted to evaluate the relationship between serum serine levels and all-cause mortality in general hypertensive patients in a longitudinal cohort, and to examine the potential effect modifiers.

Methods: A nested case-control (NCC) study was conducted utilizing 20702 hypertensive participants from the China Stroke Primary Prevention Trial (CSPPT), a randomized, double-blind, actively controlled trial conducted from May 2008 to August 2013 in China. The current study included 291 cases of all-cause mortality and 291 controls matched on age (≤ 1 year), sex and treatment group. All-cause mortality was the main outcome in this analysis, which included death due to any reason.

Results: With the increase in serum serine levels, the risk of all-cause mortality first increased before flattening. After adjusting for related variables, the risk of mortality increased significantly with the increase of serum serine levels. Compared with group Q1, the mortality risk of group Q2, Q3 and Q4 were significantly increased [ORs, 95% CI: Q2: 2.32, (1.32-4.07); Q3: 2.59, (1.48-4.54); and Q4: 1.85, (1.07-3.22)]. In the exploratory analysis, we observed three effect modifiers, total homocysteine, 5-Methyltetrahydrofolate, and estimated glomerular filtration rate significantly modified the serum serine and all-cause mortality association.

Conclusion: Serum serine levels were significantly associated with an increased risk of all-cause mortality in hypertensive patients. Our results and findings, if confirmed further, suggest that serum serine should be considered as a marker for screening risk factors of mortality.

Clinical Trial Registration: [https://www.clinicaltrials.gov/ct2/show/study/NCT00794885.], identifier [CSPPT, NCT00794885].
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http://dx.doi.org/10.3389/fnut.2022.946277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315370PMC
July 2022

Circulating TP73-AS1 and CRNDE serve as diagnostic and prognostic biomarkers for non-small cell lung cancer.

Cancer Med 2022 Jul 24. Epub 2022 Jul 24.

Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, China.

Background: Circulating long noncoding RNAs (lncRNAs) are considered a new class of biomarkers for the diagnosis and prognosis of various malignancies. We aimed to identify circulating lncRNAs as biomarkers for the diagnosis and prognosis of non-small cell lung cancer (NSCLC).

Methods: The expression of 14 candidate lncRNAs was measured in matched cancer and ipsilateral normal lung tissues of 20 patients with NSCLC using quantitative reverse-transcription PCR. In plasma samples from training and testing sets, significantly and aberrantly expressed lncRNAs, TA73-AS1 and CRNDE, were further analyzed. Receiver operating characteristic (ROC) curves were constructed, and the areas under the ROC curves (AUC) were obtained to assess diagnostic performance. The Kaplan-Meier survival analysis was used to assess the impact of plasma TA73-AS1 and CRNDE expression on tumor-free survival (TFS) of patients with NSCLC. The effect of TP73-AS1 expression on NSCLC cells was investigated in vitro.

Results: AUC values of plasma TA73-AS1 and CRNDE were 0.822 and 0.815 in the training set and 0.843 and 0.804 in the testing set, respectively, to distinguish NSCLC from healthy controls. The combination of plasma TP73-AS1, CRNDE, and two classical tumor markers, carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1), showed excellent diagnostic performance for NSCLC (AUC =0.927 in the training set; AUC = 0.925 in the testing set). Furthermore, the high expression of the two plasma lncRNAs correlated with worse TFS in patients with NSCLC. In vitro cell model studies revealed that TP73-AS1 overexpression facilitated NSCLC cell survival, invasion, and migration.

Conclusion: Circulating TP73-AS1 and CRNDE could be potential biomarkers for the diagnosis and prognostic prediction of NSCLC.
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http://dx.doi.org/10.1002/cam4.5013DOI Listing
July 2022

A sensitive UPLC-MS/MS method for simultaneous quantification of one-carbon metabolites & co-factors in human plasma.

J Pharm Biomed Anal 2022 Sep 16;219:114944. Epub 2022 Jul 16.

Jinan University College of Pharmacy, Guangzhou 510630, PR China. Electronic address:

One-carbon metabolism is an important metabolic pathway involved in many diseases, such as congenital malformations, tumours, cardiovascular diseases, anaemia, depression, cognitive diseases and liver disease. However, the current methods have specific defects in detecting and qualifying the related compounds of one-carbon metabolism. In this study, a validated method was established to simultaneously quantify 22 one-carbon metabolites & co-factors in human plasma and applied to the study of correlation between one-carbon metabolism and colorectal cancer in human plasma samples, which were from 44 healthy subjects and 55 colorectal cancer patients. The method used ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS), and the analytes included betaine, L-carnitine, L-cystathionine, L-cysteine, dimethylglycine, DL-homocysteic acid, homocysteine, methionine, pyridoxal hydrochloride, pyridoxamine dihydrochloride, pyridoxine dihydrochloride, S-(5'-Adenosyl)-L-homocysteine, serine, choline chloride, folic acid, glycine, pyridoxal phosphate monohydrate, riboflavin, taurine, 5-methyltetrahydrofolate, S-(5'-adenosyl)-L-methionine disulfate salt, trimethylamine oxide. The developed method was successfully applied to the quantification of 22 one-carbon metabolites & co-factors in human plasma from colorectal cancer patients and healthy individuals. The plasma concentrations of dimethylglycine was significantly decreased in the patients compared with the healthy individuals, while L-cystathionine was increased.
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http://dx.doi.org/10.1016/j.jpba.2022.114944DOI Listing
September 2022

Item selection methods with exposure and time control for computerized classification test.

Br J Math Stat Psychol 2022 Jul 15. Epub 2022 Jul 15.

Collaborative Innovation Center of Assessment for Basic Education Quality, Beijing Normal University, Beijing, China.

Computerized classification testing (CCT) commonly chooses items maximizing information at the cut score, which yields the most information for decision-making. However, a corollary problem is that all examinees will be given the same set of items, resulting in high test overlap rate and unbalanced item bank usage, which threatens test security. Moreover, another pivotal issue for CCT is time control. Since both the extremely long response time (RT) and large RT variability across examinees intensify time-induced anxiety, it is crucial to reduce the number of examinees exceeding the time limitation and the differences between examinees' test-taking times. To satisfy these practical needs, this paper proposes the novel idea of stage adaptiveness to tailor the item selection process to the decision-making requirement in each step and generate fresh insight into the existing response time selection method. Results indicate that a balanced item usage as well as short and stable test times across examinees can be achieved via the new methods.
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http://dx.doi.org/10.1111/bmsp.12281DOI Listing
July 2022

Another perspective to explain green tea cream: Utilizing engineered catechin-caffeine complex.

Food Res Int 2022 Aug 21;158:111542. Epub 2022 Jun 21.

Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. Electronic address:

Green tea cream, the precipitate formed in cooled green tea infusion, appears a negative effect on flavor. To explain the mechanism of green tea cream, complexations between four catechins (CATs) and caffeine (CAF) were engineered and characterized. Dynamic light scattering, high performance liquid chromatography, scanning electron microscopy, fourier-transform infrared spectroscopy, UV-visible absorption spectrum, and x-ray diffraction were used to reveal the differences between ester catechins and non-ester catechins in the dynamic complexation process, particle shape, and precipitate mechanism with caffeine. The results indicated that CATs and CAF affected each other's microenvironment in aqueous solution and Guanidine hydrochloride (GH) weakened the effect both in solution and precipitate. In addition, the 1:1 complex structure of EGC-CAF was first proved, the hydrogen bond, CH…π and π…π interaction were the forces of CAT-CAF complexes by XRD. These results provided a new perspective to the mechanism analysis of green tea cream.
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http://dx.doi.org/10.1016/j.foodres.2022.111542DOI Listing
August 2022

Efficacy and safety of sequential therapy with sorafenib and regorafenib for advanced hepatocellular carcinoma: a two-center study in China.

J Gastrointest Oncol 2022 Jun;13(3):1266-1277

Liver Cancer Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Background: Regorafenib is a standard 2nd-line treatment for patients with advanced hepatocellular carcinoma (HCC), but the efficacy and safety of sequential therapy with sorafenib and regorafenib among advanced HCC patients in China is not clear.

Methods: This was a retrospective, two-center, cohort study of advanced HCC patients who received sequential therapy of sorafenib and regorafenib from October 2018 to April 2020 at 2 Chinese institutions. The patients were converted directly to regorafenib after failing to respond to sorafenib monotherapy. The patients underwent evaluations every 4-6 weeks to determine the efficacy and safety of the treatment according to physiological, laboratory, and radiological results. A radiological evaluation using computed tomography or magnetic resonance imaging scans was conducted. The outcomes included overall survival (OS) and progression-free survival (PFS).

Results: A total of 43 patients received regorafenib as a 2nd-line treatment after sorafenib progression. Of these patients, 26 (60.5%) and 17 (39.5%) were diagnosed with Barcelona Clinic Liver Cancer (BCLC) stages B and C, respectively. The median PFS was 11.0 [95% confidence interval (CI): 5.8-16.2] months, and the median OS was 17.0 (95% CI: 12.8-21.2) months. Conversely, the most common toxicities were hand-foot skin reaction (48.8%), diarrhea (32.6%), and hypertension (14%). The most common grade 3-4 toxicities were hypoalbuminemia (4.7%), anemia (4.7%), and thrombocytopenia (4.7%). Alpha-fetoprotein (AFP) ≥400, alanine transaminase (ALT) ≥60 IU/L, and aspartate aminotransferase (AST) ≥60 IU/L before 2nd-line treatment were associated with PFS in the univariable analyses. The Cox proportional-hazards regression analysis showed that AFP [hazard ratio (HR) =0.225; 95% CI: 0.073-0.688; P=0.009], ALT (HR =0.195; 95% CI: 0.051-0.741; P=0.016), AST (HR =0.209; 95% CI: 0.063-0.697; P=0.011), and presence of extrahepatic metastasis (HR =0.074; 95% CI: 0.009-0.608; P=0.015) before 2nd-line treatment were independently associated with PFS.

Conclusions: The sequential therapy of sorafenib and regorafenib is well-tolerated and effective in advanced HCC patients after sorafenib progression based on our two-center real-world data. Patients with good liver function reserve and a high level of AFP before 2nd-line treatment may benefit from sequential treatment. These results still need further validation.
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http://dx.doi.org/10.21037/jgo-22-397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274072PMC
June 2022

Role of kinesin family member 14 in disease monitoring and prognosis in patients with gastrointestinal cancer.

Oncol Lett 2022 May 16;23(5):156. Epub 2022 Mar 16.

Department of Oncology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

Kinesin family member 14 (KIF14) is not only involved in numerous essential biological activities, such as cytokinesis and myelination, but also regulates several malignant behaviors and progression of cancer. However, its role in gastrointestinal cancer is rarely reported. Therefore, the present study aimed to investigate the association of KIF14 expression with disease-free survival (DFS) and overall survival (OS) times in patients with gastrointestinal cancer. A total of 101 patients with gastrointestinal cancer (36 patients with gastric cancer and 65 patients with colorectal cancer) were retrospectively reviewed, and their cancer samples were collected to detect the protein and mRNA expression levels of KIF14 using immunohistochemistry and reverse transcription-quantitative PCR, respectively. KIF14 protein expression was increased in cancer tissues compared with adjacent tissues (all P<0.001). The protein expression levels of KIF14 were positively associated with T stage (P<0.001), distant metastases (P=0.007) and TNM stage (P<0.001), while KIF14 mRNA expression was positively associated with T stage (P<0.001), lymph node metastasis (P=0.004), distant metastases (P=0.001) and TNM stage (P<0.001). High protein and mRNA expression levels of KIF14 were associated with worse DFS (P<0.001) and OS (P=0.016) times. In addition, high KIF14 protein expression independently predicted unfavorable DFS times (P=0.007). Subgroup analysis revealed that in patients with gastric cancer, KIF14 expression was associated with DFS and OS times, while in patients with colorectal cancer, KIF14 expression was only associated with DFS time, but not with OS time. In conclusion, KIF14 expression was not only associated with advanced pathological differentiation and TNM stage but was also associated with poor survival time in patients with gastrointestinal cancer. These results indicate the potential of KIF14 as a biomarker for gastrointestinal cancer prognosis.
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http://dx.doi.org/10.3892/ol.2022.13276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258591PMC
May 2022

Theaflavin: a natural candidate to restrain thrombosis.

Food Funct 2022 Jul 18;13(14):7572-7581. Epub 2022 Jul 18.

Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

Many clinical studies have demonstrated the beneficial effects of black tea on cardiovascular diseases. However, the antiplatelet and antithrombotic activities of theaflavin (TF-1) remain unknown. In this study, we aimed to investigate the beneficial effects of TF-1 on platelet activation and thrombosis formation both and . Firstly, the antiplatelet activity of TF-1 was analyzed using platelets isolated from human blood aggregometry, flow cytometry, the ELISA kit, western blot and fluorescence microscopy. Subsequently, the analysis of the hemostatic state and thrombosis formation was carried out in C57BL/6 mice based on the tail bleeding time and an FeCl-induced arterial thrombus model. The results showed that TF-1 could prominently inhibit platelet aggregation in a dose-dependent manner, and attenuate P-selectin expression, fibrinogen binding, spreading and thromboxane A2 (TxA2) formation. Western blot analysis showed that TF-1 potently inhibited spleen tyrosine kinase (Syk) and Akt (ser473/474) phosphorylation. The data further confirmed the inhibition of platelet activation by TF-1 with a prolonged arterial occlusion time (from 15.0 ± 1.1 minutes to 40.0 ± 5.4 minutes). All the results indicated that TF-1 is a powerful inhibitor of platelet activation and thrombosis formation in C57BL/6 mice, and could be developed as a novel food-based inhibitor of thrombotic disorders.
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http://dx.doi.org/10.1039/d2fo00152gDOI Listing
July 2022

Glaucocalyxin A Inhibits the Malignancies of Gastric Cancer Cells by Downregulating MDM2 and RNF6 MiR-3658 and the SMG1-UPF mRNA Decay Pathway.

Front Oncol 2022 22;12:871169. Epub 2022 Jun 22.

Department of Medical Affairs, Hangzhou Huqingyu Hall Pharmaceutical Co., Ltd., Hangzhou, China.

Gastric cancer (GC) ranks as the most common gastrointestinal cancer and is among the leading causes of cancer death worldwide. Glaucocalyxin A (GLA), an entkauranoid diterpene isolated from Rab-dosia japonica var., possesses various bioactivities. To date, the data on the effect of GLA on GC are still minimal, and the molecular mechanisms remain largely unknown. Herein, we found that GLA could significantly inhibit the proliferation, cell adhesion, and invasion of HGT-1, SNU-1, SNU-6, and NCI-N87 GC cells in a dose-dependent manner. GLA enhanced the apoptosis of the GC cells as evidenced by the increased caspase-3 activity and the elevated levels of cleaved caspase-3 and cleaved PARP in GC cells in the presence of GLA. We then showed that the downregulation of Murine Double Minute Clone 2 (MDM2) and Ring Finger Protein 6 (RNF6) by GLA was implicated in the GLA-induced inhibition of the GC cells. Furthermore, MDM2 and RNF6 were identified as the targets of miR-3658 that was downregulated in the GC cells and upregulated by GLA. Moreover, it was shown that miR-3658 was hypermethylated in the GC cells, and GLA could rescue the expression of miR-3658 demethylation by abrogating EZH2-mediated epigenetic silencing. In addition to the miR-3658-MDM2/RNF6 regulatory axis, activation of the SMG1-UPF mRNA decay pathway contributed to the downregulation of MDM2 and RNF6 by GLA in the GC cells. The inhibitory effect of GLA on gastric cancer and the expression of MDM2 and RNF6 was also validated in study. Our findings suggest that has the therapeutic potential for GC by downregulating oncogenes posttranscriptional regulation.
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http://dx.doi.org/10.3389/fonc.2022.871169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258495PMC
June 2022

Intermetallic PtFe Electrocatalysts for the Oxygen Reduction Reaction: Ordering Degree-Dependent Performance.

Small 2022 Aug 10;18(31):e2202916. Epub 2022 Jul 10.

Hefei National Research Center for Physical Sciences at the Microscale, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.

Platinum-based atomically ordered alloys (i.e., intermetallic compounds) have distinct advantages over disordered solid solution counterparts in boosting the cathodic oxygen-reduction reaction (ORR) in proton-exchange-membrane fuel cells. Nevertheless, the pivotal role of ordering degree of intermetallic catalysts in promoting ORR performance has been ignored heavily so far, probably owing to the lack of synthetic routes for controlling the ordering degree, especially for preparing highly ordered intermetallic catalysts. Herein, a family of intermetallic PtFe catalysts with similar particle size of 3-4 nm but varied ordering degree in a wide range of 10-70% are prepared. After constructing the PtFe/Pt core/shell structure with around 3 Pt-layer skin, a positive correlation between the ordering degree of the intermetallic catalysts and their ORR activity and durability is identified. Notably, the highly ordered PtFe/Pt catalyst exhibits a high mass activity of 0.92 A mg at 0.9 V as cathode catalyst in H -O fuel cell, with only 24% loss after accelerated durability tests. The ordering degree-dependent performance can be ascribed to the compressive strain effect induced by the intermetallic PtFe core with smaller lattice parameters, and the more thermodynamically stable intermetallic structure compared to disordered alloys.
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http://dx.doi.org/10.1002/smll.202202916DOI Listing
August 2022

A New Axial Stress Measurement Method for High-Strength Short Bolts Based on Stress-Dependent Scattering Effect and Energy Attenuation Coefficient.

Sensors (Basel) 2022 Jun 22;22(13). Epub 2022 Jun 22.

College of Mechanical Engineering, Chongqing University, Chongqing 400044, China.

The accurate estimation of axial stresses is a major problem for high-strength bolted connections that needs to be overcome to improve the assembly quality and safety of aviation structures. However, the conventional acoustoelastic effect based on velocity-stress dependence is very weak for short bolts, which leads to large estimation errors. In this article, the effect of axial stress on ultrasonic scattering attenuation is investigated by calculating the change in the energy attenuation coefficient of ultrasonic echoes after applying axial preload. Based on this effect, a stress-dependent attenuation estimation model is developed to measure the bolt axial stress. In addition, the spectrum of the first and second round-trip echoes is divided into several frequency bands to calculate the energy attenuation coefficients, which are used to select the frequency band sensitive to the axial stress changes. Finally, the estimation model between axial stress and energy attenuation coefficients in the sensitive frequency band is established under 20 steps of axial preloads. The experimental results show that the energy attenuation coefficient in the sensitive band corresponds well with axial stress. The average relative error of the predicted axial stress is 6.28%, which is better than that of the conventional acoustoelastic effect method. Therefore, the proposed approach can be used as an effective method to measure the axial stress of short bolts in the assembly of high-strength connections.
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http://dx.doi.org/10.3390/s22134692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269293PMC
June 2022

Increased Incidence of Hashimoto Thyroiditis in Selenium Deficiency: A Prospective 6-Year Cohort Study.

J Clin Endocrinol Metab 2022 Aug;107(9):e3603-e3611

Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, 710061, China.

Context: In 2015, we reported an increased prevalence of thyroid disease in a county of low habitual selenium (Se) intake in comparison to a neighboring county with higher intake in a cross-sectional survey in Shaanxi Province, China.

Objective: To explore longitudinal effects of low Se status, a prospective cohort study was conducted in the same area from 2013 to 2019, and thyroid peroxidase autoantibodies (TPO-Abs) and disease incidence were compared.

Methods: A total 1254 individuals from 1500 reinvited participants were successfully enrolled. Venous blood, fingernails, and urine samples were collected and analyzed to evaluate thyroid status, TPO-Abs, serum Se, and urinary iodine. Diagnosis of Hashimoto thyroiditis (HT) was based on elevated thyrotropin, presence of TPO-Abs, and ultrasound characteristics. Se deficiency was categorized using a serum concentration of 80 µg/L as a threshold, and tested by logistic regression for a relationship to TPO-Abs and HT.

Results: Se deficiency was observed in 46.2% of participants from the adequate-Se county (Ziyang) and in 89.7% from the low-Se county (Ningshan). Se concentrations in fingernails differed strongly by residency (Ziyang vs Ningshan; 678.7 vs 364.3 μg/kg; Z = -9.552; P < .001). Newly diagnosed HT in Ziyang was less frequent than in Ningshan (0.09% vs 0.31%; χ 2 = 4.350; P = .037). The conversion rate to seropositive TPO-Abs was 10.2% in Ningshan vs 5.6% in Ziyang. Excluding iodine as confounding factor, low-Se was confirmed as a risk factor for HT (relative risk [95% CI]; 3.65 [1.03-12.90]; P < .05).

Conclusion: The data indicate an increased incidence of TPO-Ab seroconversion with low Se supply and support the hypothesis that Se deficiency contributes to HT as a modifiable risk factor.
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http://dx.doi.org/10.1210/clinem/dgac410DOI Listing
August 2022
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