Publications by authors named "Pin Lv"

98 Publications

Orf virus ORF120 protein positively regulates the NF-κB pathway by interacting with G3BP1.

J Virol 2021 Jul 21:JVI0015321. Epub 2021 Jul 21.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Orf virus (ORFV) is a highly epitheliotropic parapoxvirus with zoonotic significance that induces proliferative lesions in the skin of sheep, goats and humans. Several viral proteins encoded by ORFV, including NF-κB inhibitors, play important roles in hijacking host-associated proteins for viral evasion of the host innate immune response. However, the roles of proteins with unknown functions in viral replication and latent infection remain to be explored. Here, we present data demonstrating that the ORF120, an early-late ORFV encoded protein, activates the nuclear factor-κB (NF-κB) pathway in the early phase of infection, which implies that ORFV may regulate NF-κB through a biphasic mechanism. DUAL membrane yeast two-hybrid system and coimmunoprecipitation experiments revealed that the ORF120 protein interacts with Ras-GTPase-activating protein (SH3 domain) binding protein 1 (G3BP1). The overexpression of the ORF120 protein can efficiently increase the expression of G3BP1 and nuclear translocation of NF-κB-p65 in OFTu and HeLa cells. The knockdown of G3BP1 significantly decreased ORF120-induced NF-κB activation, indicating that G3BP1 is involved in ORF120-induced NF-κB pathway activation. Dual-luciferase reporter assay revealed that ORF120 could positively regulate the NF-κB pathway through the full-length G3BP1 or the domain of G3BP1. In conclusion, we demonstrate, for the first time, that the ORF120 protein is capable of positively regulating NF-κB signaling by interacting with G3BP1, providing new insights into ORFV pathogenesis and a theoretical basis for antiviral drug design. As part of the host innate response, the NF-κB pathway plays a partial antiviral role in nature by regulating the innate immune response. Thus, the NF-κB pathway is probably the most frequently targeted intracellular pathway for subversion by anti-immune modulators that are encoded by a wide range of pathogens. Various viruses, including Poxviruses, encode several proteins that prepare the host cell for viral replication by inhibiting cytoplasmic events, leading to the initiation of NF-κB transcriptional activity. However, NF-κB activity is hypothesized to facilitate viral replication to a great extent. The significance of our research is in the exploration of the activation mechanism of NF-κB induced by the ORFV ORF120 protein interacting with G3BP1, which helps not only to explain the ability of ORFV to modulate the immune response through the positive regulation of NF-κB but also to show the mechanism by which the virus evades the host innate immune response.
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http://dx.doi.org/10.1128/JVI.00153-21DOI Listing
July 2021

ETS like-1 protein ELK1-induced lncRNA LINC01638 accelerates the progression of papillary thyroid cancer by regulating Axin2 through Wnt/β-catenin signaling pathway.

Authors:
Pin Lv Yuan Xue

Bioengineered 2021 Dec;12(1):3873-3885

The General Surgery Department, The Second Hospital of the University of ShanXi, Taiyuan, Shanxi Province, China.

Papillary thyroid carcinoma (PTC) characterized by distant metastasis is a major public health issue among women worldwide. LncRNA LINC01638 is reportedly a critical oncogene in the development of certain cancers. However, the biological function of LINC01638 in PTC is currently unclear. The goal of this study was to identify LINC01638 expression level and its role in PTC progression. The expression of LINC01638 was detected applying qRT-PCR. CCK-8 assay, colony formation assay, immunofluorescence staining and flow cytometric analysis were performed to assess cell proliferation and cell cycle. In addition, cell migration and invasion were examined via wound healing assay, transwell assay and western blot analysis. We found that LINC0163 was upregulated in PTC cells compared with normal thyroid gland epithelial cell line Nthy-ori3-1. ELK1 could act as a transcription factor of LINC01638 and induce LINC01638 expression. LINC01638 silencing inhibited cell proliferation, migration and invasion, and obstructed the progress of TPC-1 cell cycle. LINC0163 silencing activated Axin2 while suppressing the expressions of β-catenin, Cyclin-D1 and c-MYC. Rescue experiment utilizing the transfection of Axin2 overexpression plasmid weakened LINC01638 overexpression-enhanced TPC-1 cell proliferation, metastasis, cell cycle progress and Wnt/β-catenin pathway. These results indicate that LINC0163 regulates PTC progression via inhibition of Wnt/β-catenin and activation of Axin2, which may develop into a novel therapeutic strategy for PTC treatment.
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http://dx.doi.org/10.1080/21655979.2021.1935404DOI Listing
December 2021

Ultrasmall Fe(III)-Tannic Acid Nanoparticles To Prevent Progression of Atherosclerotic Plaques.

ACS Appl Mater Interfaces 2021 Jul 19. Epub 2021 Jul 19.

Department of Radiology, Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.

Macrophage accumulation is central to the pathogenesis of atherosclerotic plaques. Reducing macrophages in plaques is an appealing approach to attenuate the development of atherosclerosis. Chemodynamic therapy, specifically inhibiting hydrogen peroxide (HO)-rich cells in slightly acidic microenvironment, has emerged as a new method in tumor treatment. Herein, we manufactured ultrasmall dopamine-modified hyaluronic acid (HD)-stabilized Fe(III)-tannic acid nanoparticles (HFTNPs). HFTNPs can specifically accumulate in inflammatory macrophages in atherosclerotic plaques, provide brighter magnetic resonance images, promote reactive oxygen species (ROS) generation, and induce the death of inflammatory macrophages without damaging normal cells and tissues. In conclusion, HFTNPs have a tremendous potential as safe and effective diagnostic and therapeutic reagents for atherosclerosis.
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http://dx.doi.org/10.1021/acsami.1c09480DOI Listing
July 2021

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities.

Front Environ Sci Eng 2022 30;16(3):35. Epub 2021 Jun 30.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084 China.

The distribution of antibiotic resistance genes (ARGs) has been intensively studied in large-scale wastewater treatment plants and livestock sources. However, small-scale decentralized sewage treatment facilities must also be explored due to their possible direct exposure to residents. In this study, six wastewater treatment facilities in developed rural areas in eastern China were investigated to understand their risks of spreading ARGs. Using metagenomics and network analysis tools, ARGs and bacterial and viral communities were identified in the influent (INF) and effluent (EFF) samples. The dominant ARGs belonged to the bacitracin class, which are different from most of municipal wastewater treatment plants (WWTPs). The dominant hosts of ARGs are in bacterial communities and in viral communities. Furthermore, a positive relationship was found between ARGs and phages. The ARGs significantly correlated with phages were all hosted by specific genera of bacteria, indicating that phages had contributed to the ARG's proliferation in sewage treatment facilities. Paying significant concern on the possible enhanced risks caused by bacteria, viruses and their related ARGs in decentralized sewage treatment facilities is necessary.

Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s11783-021-1469-4 and is accessible for authorized users.
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http://dx.doi.org/10.1007/s11783-021-1469-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255336PMC
June 2021

Statistical Confirmation of a Method of US Determination of Bone Age.

Radiology 2021 Jul 18;300(1):176-183. Epub 2021 May 18.

From the Departments of Pathology (J.W.) and Medical Ultrasound (Y.Z., Q.F., P.L., K.H., C.Z.), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Rd, Wuhan 430030, China.

Background There is limited literature on conventional US to assess bone age. Purpose To determine the diagnostic performance of US in the assessment of abnormal bone age in Chinese children. Materials and Methods In this prospective study, children and young adults aged between birth and 19 years from a large provincial teaching hospital were enrolled from January to November 2020. Children without clinical diseases potentially affecting skeletal growth were included in the normal-value group. Children with clinically suspected growth disturbances who were undergoing bone age evaluation were included in the validation group. Ossification ratios (ie, the ratio of the height of the epiphyseal ossification center to the entire epiphysis, including the cartilaginous component) of the radius, ulna, and femur from all the children were measured using US. Ultrasonic skeletal maturity scores (ie, the summation of ossification ratios of the radius, ulna, and femur multiplied by 100) collected from children in the normal-value group were used for score-for-age curve fitting through Box-Cox power exponential distribution. Test performance characteristics for the ability of US to help diagnose abnormal bone age were determined using radiographic bone age as a reference standard. Statistically significant difference between groups was determined by using a paired-sample test. Results A total of 1089 children (median age, 9 years [interquartile range, 3-14 years]; 578 boys) were enrolled, including 929 children (mean age, 8 years [interquartile range, 4-12 years]; 515 boys) in the normal-value group and 160 children (mean age, 9 years [interquartile range, 7-11 years]; 63 boys) in the validation group. Ultrasonic bone ages in the validation group were evaluated with use of the lists of normal score-for-age values. With radiographic bone age as a reference standard, US could help diagnose abnormal bone age with high sensitivity (93% [14 of 15 participants; 95% CI: 66, 100] for boys, 100% [14 of 14 participants; 95% CI: 73, 100] for girls) and specificity (98% [47 of 48 participants; 95% CI: 88, 100] for boys, 98% [81 of 83 participants; 95% CI: 91, 100] for girls). Conclusion The US scoring system established can be used to evaluate bone age with high sensitivity and specificity. Clinical trial registration no. ChiCTR1900027917 © RSNA, 2021
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http://dx.doi.org/10.1148/radiol.2021204353DOI Listing
July 2021

Causal structural covariance network revealing atrophy progression in Alzheimer's disease continuum.

Hum Brain Mapp 2021 Aug 12;42(12):3950-3962. Epub 2021 May 12.

Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

The structural covariance network (SCN) has provided a perspective on the large-scale brain organization impairment in the Alzheimer's Disease (AD) continuum. However, the successive structural impairment across brain regions, which may underlie the disrupted SCN in the AD continuum, is not well understood. In the current study, we enrolled 446 subjects with AD, mild cognitive impairment (MCI) or normal aging (NA) from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. The SCN as well as a casual SCN (CaSCN) based on Granger causality analysis were applied to the T1-weighted structural magnetic resonance images of the subjects. Compared with that of the NAs, the SCN was disrupted in the MCI and AD subjects, with the hippocampus and left middle temporal lobe being the most impaired nodes, which is in line with previous studies. In contrast, according to the 194 subjects with records on CSF amyloid and Tau, the CaSCN revealed that during AD progression, the CaSCN was enhanced. Specifically, the hippocampus, thalamus, and precuneus/posterior cingulate cortex (PCC) were identified as the core regions in which atrophy originated and could predict atrophy in other brain regions. Taken together, these findings provide a comprehensive view of brain atrophy in the AD continuum and the relationships among the brain atrophy in different regions, which may provide novel insight into the progression of AD.
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http://dx.doi.org/10.1002/hbm.25531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288084PMC
August 2021

Multiscale investigation on the chemical and anatomical changes of lignocellulosic biomass for different severities of hydrothermal treatment.

Sci Rep 2021 Apr 19;11(1):8444. Epub 2021 Apr 19.

INRAE, AgroParisTech, UMR SayFood, Université Paris-Saclay, 91300, Massy, France.

The chemical changes sustained by lignocellulosic biomass during hydrothermal treatment are reflected at multiple scales. This study proposes to benefit from this multiscale nature in order to provide a global understanding of biomass alterations during hydrothermal treatment. For this purpose, complementary imaging techniques-confocal Raman microscopy and X-ray nano-tomography-analysed by image processing and coupled to chemical measurements were used. This unique combination of analyses provided valuable information on topochemical and morphological changes of poplar samples, without the artefacts of sample preparation. At the cell wall level, holocellulose hydrolysis and lignin modifications were observed, which corresponded to anatomical modifications observed at higher scales. Overall, after treatment, samples shrank and had thinner cell walls. When subjected to more severe pre-treatments, cells were disrupted and detached from adjacent cells. Anatomical changes were then used to obtain quantitative indicators of the treatment severity. The effects of treatment at different scales can thus be quantitatively connected in both directions, from micro to macro and from macro to micro.
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http://dx.doi.org/10.1038/s41598-021-87928-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055998PMC
April 2021

SM22 Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B.

Oxid Med Cell Longev 2021 16;2021:5564884. Epub 2021 Mar 16.

Department of Biochemistry and Molecular Biology, College of Basic Medicine, Key Laboratory of Medical Biotechnology of Hebei Province, Hebei Medical University, Shijiazhuang, Hebei 050017, China.

Vascular smooth muscle cell (VSMC) apoptosis is a major defining feature of abdominal aortic aneurysm (AAA) and mainly caused by inflammatory cell infiltration. Smooth muscle (SM) 22 prevents AAA formation through suppressing NF-B activation. However, the role of SM22 in VSMC apoptosis is controversial. Here, we identified that SM22 loss contributed to apoptosis of VSMCs via activation of macrophages. Firstly, deficiency of SM22 enhanced the interaction of VSMCs with macrophages. Macrophages were retained and activated by VSMCs via upregulating VCAM-1 expression. The ratio of apoptosis was increased by 1.62-fold in VSMCs treated with the conditional media (CM) from activated RAW264.7 cells, compared to that of the control CM ( < 0.01), and apoptosis of VSMCs was higher than that of WT VSMCs ( < 0.001). Next, circRasGEF1B from activated macrophages was delivered into VSMCs promoting ZFP36 expression via stabilization of ZFP36 mRNA. Importantly, circRasGEF1B, as a scaffold, guided ZFP36 to preferentially bind to and decay Bcl-2 mRNA in a sequence-specific manner and triggered apoptosis of VSMCs, especially in VSMCs. These findings reveal a novel mechanism by which the circRasGEF1B-ZFP36 axis mediates macrophage-induced VSMC apoptosis via decay of Bcl-2 mRNA, whereas VSMCs have a higher sensitivity to apoptosis.
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http://dx.doi.org/10.1155/2021/5564884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026322PMC
June 2021

Prospective multicenter study on the incidence of surgical site infection after emergency abdominal surgery in China.

Sci Rep 2021 Apr 8;11(1):7794. Epub 2021 Apr 8.

Department of Emergency Medicine, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266000, People's Republic of China.

There is still a lack of relevant studies on surgical site infection (SSI) after emergency abdominal surgery (EAS) in China. This study aims to understand the incidence of SSI after EAS in China and discuss its risk factors. All adult patients who underwent EAS in 47 hospitals in China from May 1 to 31, 2018, and from May 1 to June 7, 2019, were enrolled in this study. The basic information, perioperative data, and microbial culture results of infected incision were prospectively collected. The primary outcome measure was the incidence of SSI after EAS, and the secondary outcome variables were postoperative length of stay, ICU admission rate, ICU length of stay, 30-day postoperative mortality, and hospitalization cost. Univariate and multivariate logistic regression were used to analyze the risk factors. The results were expressed as the odds ratio and 95% confidence interval. A total of 953 patients [age 48.8 (SD: 17.9), male 51.9%] with EAS were included in this study: 71 patients (7.5%) developed SSI after surgery. The main pathogen of SSI was Escherichia coli (culture positive rate 29.6%). Patients with SSI had significantly longer overall hospital (p < 0.001) and ICU stays (p < 0.001), significantly higher ICU admissions (p < 0.001), and medical costs (p < 0.001) than patients without SSI. Multivariate logistic regression analysis showed that male (P = 0.010), high blood glucose level (P < 0.001), colorectal surgery (P < 0.001), intestinal obstruction (P = 0.045) and surgical duration (P = 0.007) were risk factors for SSI, whereas laparoscopic surgery (P < 0.001) was a protective factor. This study found a high incidence of SSI after EAS in China. The occurrence of SSI prolongs the patient's hospital stay and increases the medical burden. The study also revealed predictors of SSI after EAS and provides a basis for the development of norms for the prevention of surgical site infection after emergency abdominal surgery.
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http://dx.doi.org/10.1038/s41598-021-87392-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032698PMC
April 2021

Ego- and allo-network disconnection underlying spatial disorientation in subjective cognitive decline.

Cortex 2021 04 25;137:35-48. Epub 2021 Jan 25.

Department of Radiology, Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China; Department of Radiology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, China; Institute of Brain Science, Nanjing University, Nanjing, 210008, China. Electronic address:

Patients with Alzheimer's disease (AD) related dementia and mild cognitive impairment experience difficulties with spatial navigation (SN). However, SN has rarely been investigated in individuals with subjective cognitive decline (SCD), a preclinical stage with elevated progression rate to symptomatic AD. In this study, 30 SCD subjects and 30 controls underwent cognitive scale (CS) evaluation, a 2D computerized SN test, and resting-state functional magnetic resonance imaging scanning. Two SN brain networks (ego-network and allo-network), each with 10 selected spherical regions, were defined. We calculated the average network functional connectivity (FC) and region-to-region FC within the two networks and evaluated correlations with SN performance. Compared with the controls, the SCD group performed worse in the SN test and showed decreased FC between the right retrosplenial and right prefrontal cortices in the ego-network, and between the right retrosplenial cortex and right hippocampus in the allo-network. The logistic regression model based on SN and FC measures revealed a high area under the curve of .880 in differentiating SCD individuals from controls. These results suggest that SN network disconnection contributes to spatial deficits in SCD, and SN and FC measures could benefit the preclinical detection of subjects with incipient AD dementia.
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http://dx.doi.org/10.1016/j.cortex.2020.12.022DOI Listing
April 2021

Deep Learning-Based Classification and Voxel-Based Visualization of Frontotemporal Dementia and Alzheimer's Disease.

Front Neurosci 2020 21;14:626154. Epub 2021 Jan 21.

National Institute of Healthcare Data Science at Nanjing University, Nanjing, China.

Frontotemporal dementia (FTD) and Alzheimer's disease (AD) have overlapping symptoms, and accurate differential diagnosis is important for targeted intervention and treatment. Previous studies suggest that the deep learning (DL) techniques have the potential to solve the differential diagnosis problem of FTD, AD and normal controls (NCs), but its performance is still unclear. In addition, existing DL-assisted diagnostic studies still rely on hypothesis-based expert-level preprocessing. On the one hand, it imposes high requirements on clinicians and data themselves; On the other hand, it hinders the backtracking of classification results to the original image data, resulting in the classification results cannot be interpreted intuitively. In the current study, a large cohort of 3D T1-weighted structural magnetic resonance imaging (MRI) volumes ( = 4,099) was collected from two publicly available databases, i.e., the ADNI and the NIFD. We trained a DL-based network directly based on raw T1 images to classify FTD, AD and corresponding NCs. And we evaluated the convergence speed, differential diagnosis ability, robustness and generalizability under nine scenarios. The proposed network yielded an accuracy of 91.83% based on the most common T1-weighted sequence [magnetization-prepared rapid acquisition with gradient echo (MPRAGE)]. The knowledge learned by the DL network through multiple classification tasks can also be used to solve subproblems, and the knowledge is generalizable and not limited to a specified dataset. Furthermore, we applied a gradient visualization algorithm based on guided backpropagation to calculate the contribution graph, which tells us intuitively why the DL-based networks make each decision. The regions making valuable contributions to FTD were more widespread in the right frontal white matter regions, while the left temporal, bilateral inferior frontal and parahippocampal regions were contributors to the classification of AD. Our results demonstrated that DL-based networks have the ability to solve the enigma of differential diagnosis of diseases without any hypothesis-based preprocessing. Moreover, they may mine the potential patterns that may be different from human clinicians, which may provide new insight into the understanding of FTD and AD.
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http://dx.doi.org/10.3389/fnins.2020.626154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858673PMC
January 2021

Cyclodextrin pendant polymer as an efficient drug carrier for scutellarin.

Drug Deliv 2020 Nov;27(1):1741-1749

Pharmacy Department, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing, P.R. China.

A novel β-cyclodextrin pendant polymer (ε-PL-CD), composed of poly(ε-lysine) (ε-PL) main chain and glycine-β-cyclodextrin (Gly-CD) side chains, was prepared by a simple two-step procedure. The ε-PL-CD was investigated as a drug carrier of hydrophobic drug scutellarin (SCU). The characterization and complexation mode of the SCU:ε-PL-CD were researched in both solution and solid state by means of photoluminescence spectroscopy, H and 2D NMR, X-Ray powder diffraction (XRPD), thermal gravimetric analysis, Particle size and Zeta potential. The solubility test indicated that the solubilizing ability of SCU:ε-PL-CD was significantly improved compared with SCU:β-CD and free SCU. Besides, in cell experiment, it was found that SCU:ε-PL-CD has a strong inhibitory effect on the growth and invasion of tumor cells. The present study provides useful information for ε-PL-CD as a drug carrier material.
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http://dx.doi.org/10.1080/10717544.2020.1856223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738300PMC
November 2020

Long Non-Coding RNA NCK1-AS1 Serves an Oncogenic Role in Gastric Cancer by Regulating miR-137/NUP43 Axis.

Onco Targets Ther 2020 6;13:9929-9939. Epub 2020 Oct 6.

Department of General Surgery, The Second Hospital of Shanxi Medical University, Taiyuan 030001, People's Republic of China.

Introduction: Long non-coding RNA (lncRNA) NCK1-AS1 could regulate multiple cancer progression. However, little is known regarding the roles and acting mechanisms of NCK-AS1 in gastric cancer (GC) progression. This work was aimed to explore the relationship between NCK1-AS1 and GC progression to illustrate the mechanisms of NCK1-AS1.

Methods: NCK1-AS1 expression level in GC tissues and cells was measured with a quantitative real-time PCR method. In vitro experiments including cell counting kit-8 assay, colony formation assay, wound-healing assay, and transwell invasion assay were employed to detect biological roles of NCK1-AS1 in GC progression. In vivo experiments were performed to analyze the roles of NCK1-AS1 on GC malignant phenotype. Moreover, mechanisms behind the biological roles of NCK1-AS1 in GC were investigated using bioinformatic analysis, luciferase activity reporter assay, RNA immunoprecipitation assay, and rescue experiments.

Results: NCK1-AS1 was found to have elevated expression in GC tissues and cells in comparison with normal counterparts. Loss-of-function experiments showed knockdown of NCK1-AS1 refrained GC cell proliferation, colony formation, migration, and invasion in vitro. Animal experiments showed silence of NCK1-AS1 suppresses tumor growth in vivo. Functionally, NCK1-AS1 serves as a sponge for microRNA-137 (miR-137) to upregulate nucleoporin 43 (NUP43) expression in GC. Rescue experiments proved the carcinogenic role of NCK1-AS1/miR-137/NUP43 axis in GC progression.

Discussion: In conclusion, the NCK1-AS1/miR-137/NUP43 axis was identified that could contribute to GC malignancy behaviors.
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http://dx.doi.org/10.2147/OTT.S259336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547806PMC
October 2020

A long non-coding RNA specifically expressed in early embryos programs the metabolic balance in adult mice.

Biochim Biophys Acta Mol Basis Dis 2021 01 13;1867(1):165988. Epub 2020 Oct 13.

Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200438, China. Electronic address:

Many Long non-coding RNAs (lncRNAs) are specifically expressed in early embryos, but the physiological functions of most of them remain largely unknown. Here, we show that deficiency of lncenc1, an early embryo-specific lncRNA, altering glucose and lipid balance in adult mice. Newly weaned lncenc1-deficient mice prefer to use lipids as a fuel source. When mice were fed a normal chow diet (NCD), glucose intolerance and insulin resistance were observed in adult lncenc1-deficient mice. Under high-fat diet (HFD) conditions, however, lncenc1-deficient mice became healthier and could resist food-induced obesity and metabolic disturbances. Furthermore, AKT/mTOR-regulated lipogenesis in liver was reduced in lncenc1-deficient mice fed a HFD. MEFs lacking lncenc1 showed impaired glycolysis and lipogenesis, suggesting that the metabolic defects may already exist in embryos. Our study demonstrated the essential roles of lncenc1 in adult metabolism, providing experimental data that support the "fetal origin" of adult metabolic disorders.
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http://dx.doi.org/10.1016/j.bbadis.2020.165988DOI Listing
January 2021

Bioinspired Surfaces With Switchable Wettability.

Front Chem 2020 12;8:692. Epub 2020 Aug 12.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, China.

The surface wettability of plants exhibits many unique advantages, which enhances the environmental adaptability of plants. In view of the rapid development of responsive materials, smart surfaces have been explored extensively to regulate surface wettability through external stimuli. Herein, we summarized recent advancements in bioinspired surfaces with switchable wettability. Typical bioinspired surfaces with switchable wettability and their emerging applications have been reviewed. In the end, we have discussed the remaining challenges and provided perspective on future development.
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http://dx.doi.org/10.3389/fchem.2020.00692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434979PMC
August 2020

Ag nanoparticle ink coupled with graphene oxide cellulose paper: a flexible and tunable SERS sensing platform.

Opt Lett 2020 Aug;45(15):4208-4211

Surface-enhanced Raman scattering (SERS) is highly promising for ultra-sensitive detection in a series of applications. Although extensive advances have been achieved in SERS technologies, the preparation of highly efficient SERS substrates still suffers from several limitations, including complex preparation procedures, high cost, and instability for long time storage. To address these problems, we report a novel, to the best of our knowledge, SERS platform that combines graphene oxide (GO) and cellulose composite paper with colloidal silver nanoparticle (Ag NP) ink. As an efficient substrate, the GO and cellulose composite paper that features hierarchical micro-nanostructures and improved interaction with target molecules can be fabricated on a large scale, and the Ag NP ink can be well stored, avoiding being oxidized in ambient conditions. In this way, our SERS platform not only reduces the cost, but also improved the stability. The sensitivity, reproducibility, and tunable SERS detection performance were evaluated using rhodamine 6G as probing molecules. To demonstrate the capability of our SERS platform in practical analysis, the SERS spectra of two monosodium salt solutions of different concentrations have been collected. The SERS platform has revealed great potential for practical application of SERS technologies.
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http://dx.doi.org/10.1364/OL.400131DOI Listing
August 2020

Humus formation driven by ammonia-oxidizing bacteria during mixed materials composting.

Bioresour Technol 2020 Sep 7;311:123500. Epub 2020 May 7.

Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin 150030, China.

The aim of this study was to identify the effects of ammonia-oxidizing bacteria (AOB) inoculation on humus formation. Both nitrogen conversion and humus formation were considered as the main processes, because NH-N-like compounds not only substrates of nitrification, but also precursors of humus. During composting, the inoculation of AOB indeed increased humus concentration by fixing NH emission as NH-N, but it has also promoted nitrogen transformation. While the main reason was the changed bacteria community structure caused by inoculating AOB. Moreover, the relationship between bacteria and nitrogen transformation and humus formation has become closer. And bacteria were more likely to synthesize humus. Therefore, it is conjectured that AOB inoculation could not only provide NH-N for humus formation, but also enhance the anabolism of microorganisms. This suppose has been confirmed by structural equation model in this study. Therefore, AOB inoculation has a driving effect on promoting humus formation.
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http://dx.doi.org/10.1016/j.biortech.2020.123500DOI Listing
September 2020

Author Correction: The roles of p38 MAPK → COX2 and NF-κB → COX2 signal pathways in age-related testosterone reduction.

Sci Rep 2020 Mar 10;10(1):4652. Epub 2020 Mar 10.

Department of cell biology, Hebei Medical University, Shijiazhuang, 050017, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-61687-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064487PMC
March 2020

miR-373 inhibits autophagy and further promotes apoptosis of cholangiocarcinoma cells by targeting ULK1.

Kaohsiung J Med Sci 2020 Jun 3;36(6):429-440. Epub 2020 Mar 3.

Institute of Clinical Medicine, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan Province, People's Republic of China.

Intrahepatic cholangiocarcinoma is a malignant tumor originating from intrahepatic bile ducts. Surgical therapy, radiotherapy, and chemotherapy are taken to treat this disease, but it is prone to recurrence and metastasis, with poor prognosis. Therefore, it is of great significance to explore new targets and molecular mechanisms for the development of cholangiocarcinoma cells. Clinical cholangiocarcinoma tissues from patients and four human cholangiocarcinoma cell lines were analyzed for microRNA-373 (miR-373) expression. For investigating whether miR-373 directly modulated unc-51 like autophagy activating kinase 1 (ULK1), dual-luciferase reporter assay was performed. In addition, CCK-8 assay, flow cytometry, western blot, and immunofluorescence were applied to evaluate the proliferation, apoptosis, and autophagy of cholangiocytic hepatocellular carcinoma cells. miR-373 downregulation was observed in clinical tissues and cell lines of cholangiocarcinoma. Overexpression of miR-373 reduced proliferation, enhanced apoptosis, and raised expression levels of pro-apoptosis proteins including BCL2 associated X (Bax), Caspase-3, and Caspase-9. Moreover, overexpression of miR-373 downregulated expression levels of microtubule-associated protein 1A/1B-light chain 3 (LC3)-II, Beclin-1, and promoted P62 expression on mRNA and protein levels. After miR-373 knockdown, all indexes of apoptosis and autophagy mentioned above were reversed. Luciferase activity was decreased after cotransfection of miR-373 mimic and wild-type ULK1 vector. Also, miR-373 overexpression inhibited ULK1 expression. Importantly, overexpression of miR-373 weakened expressions of ULK1, LC3, Beclin-1, and Bcl-2, and enhanced expressions of P62, Bax, Caspase-3, and Caspase-9. miR-373 mimic treatment and subsequent ULK1 overexpression, induced reverse regulation in expressions of these proteins, compared with overexpression of miR-373 only. miR-373 targeted ULK1 to initiate inhibition of autophagy and subsequent promotion of apoptosis in cholangiocarcinoma cells.
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http://dx.doi.org/10.1002/kjm2.12191DOI Listing
June 2020

Comparison of Fracture Load and Surface Wear of Microhybrid Composite and Ceramic Occlusal Veneers.

J Prosthodont 2020 Jun 24;29(5):387-393. Epub 2020 Mar 24.

Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

Purpose: To compare in vitro fracture load, surface wear, and roughness after thermal cycling and cyclic mechanical fatigue loading among cemented microhybrid resin-based composite and ceramic occlusal veneers fabricated at two thicknesses (1.5 and 2.5 mm).

Materials And Methods: Sixty-four extracted premolars without root canal treatment were prepared and restored with occlusal veneers of two thicknesses (1.5 and 2.5 mm), using four different materials: microhybrid composite (MC), fiber-reinforced microhybrid composite (FMC), heat-pressed lithium disilicate ceramic (HPC), and computer-aided design/computer-aided manufactured lithium disilicate ceramic (CCC). The specimens underwent thermal cycling and cyclic mechanical fatigue loading, and were then subjected to fracture testing, with loads at failure recorded as fracture load. Wear and surface roughness were recorded before and after fatigue loading. Results were analyzed using one-way ANOVA, two-way ANOVA (α = 0.05).

Results: All specimens survived thermal cycling and cyclic mechanical fatigue loading. At 1.5-mm thickness, the mean fracture load of FMC was highest (3926.48 ± 556.54 N), while that of CCC was highest (3066.45 ± 559.94 N) at 2.5 mm. Regardless of thickness, the fracture load of CCC was higher than that of HPC (p = 0.004 and p = 0.023). The wear of MC and FMC was significantly higher than those of HPC and CCC (p ≤ 0.001), but was similar in terms of the wear rate of tooth enamel. HPC exhibited the lowest surface roughness after fatigue loading (p ≤ 0.001).

Conclusion: All tested occlusal veneers exhibited a fracture load considerably exceeding the maximum occlusal force in the posterior dentition. When the attainable space for restoration varies, different occlusal veneer materials should be considered. The surface wear and roughness also need to be considered when selecting materials.
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http://dx.doi.org/10.1111/jopr.13156DOI Listing
June 2020

Pressure-induced unexpected -2 oxidation states of bromine and superconductivity in magnesium bromide.

Phys Chem Chem Phys 2020 Feb 22;22(5):3066-3072. Epub 2020 Jan 22.

Laboratory of High Pressure Physics and Material Science, School of Physics and Physical Engineering, Qufu Normal University, Qufu, 273165, P. R. China.

Finding novel compounds with unusual crystal structures and physical properties is always an important goal for the materials and chemistry community. Pressure becomes attractive due to its unique ability to break down many fundamental rules by modifying the chemical properties of elements, overcoming reaction barriers and shortening interatomic distances, leading to the formation of some novel materials with unexpected properties. In this work, for the first time we have analyzed the high-pressure phase diagram, crystal structures and electron properties of the Mg-Br system up to 200 GPa using unbiased structure searching techniques. Besides the already known MgBr, here we report that three unusual stoichiometries of Mg-Br compounds can be stabilized at high pressures as MgBr, MgBr and MgBr. Firstly, among the predicted stable compounds, we find that the MgBr in the I4/mmm structure stabilized at 178 GPa behaves as a typical electride, indicating that the formation pressure of an electride for Mg can be significantly reduced by bonding with Br atoms. Secondly, it is surprising that the unexpected oxidation states of Br approaching -2 are observed in the predicted I4/mmm MgBr and Pm3[combining macron]m MgBr compounds. Furthermore, P2/m MgBr and I4[combining macron]2m MgBr phases are predicted as superconductors with an estimated T of 23.2 and 0.49 K, respectively. Our work represents a significant step toward understanding the high pressure behaviors of alkaline earth halides and searching for novel high temperature superconductors.
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http://dx.doi.org/10.1039/c9cp05627kDOI Listing
February 2020

Chronic stress reduces spermatogenic cell proliferation in rat testis.

Int J Clin Exp Pathol 2019 1;12(5):1921-1931. Epub 2019 May 1.

The Fifth People's Hospital of Shanghai, Institutes of Biomedical Sciences, Fudan University Shanghai 200032, China.

Male reproductive dysfunction induced by mental stress and environmental factors has increased greatly in recent years. Previous studies of the male rat reproductive system under stress conditions evaluated changes in physiology and pathophysiology. However, no genome-wide study has been applied to such models. Here we studied the histopathologic changes in testes of rats under different durations of stress and used RNA sequencing (RNA-seq) to investigate the testicular transcriptome and detect differentially expressed genes. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry were used to verify these. Chronic stress resulted in significant histopathologic changes in seminiferous tubules and RNA-seq showed that growing numbers of genes were dysregulated with increasing stress exposure. Gene Ontology (GO) analysis showed that many biological processes of cell proliferation-associated terms were highly significantly enriched among downregulated genes, from chronically stressed groups. Proliferating cell nuclear antigen (PCNA) was used as a key marker of cell proliferation. RT-qPCR and immunohistochemistry indicated that PCNA mRNA and protein expression levels were greatly decreased with prolonged stress, thereby contributing to the attenuation of spermatogenic cell proliferation in the rat testis. This could provide a new scientific basis for the study of male reproductive dysfunction caused by stress.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947124PMC
May 2019

Effect of manganese dioxide on the formation of humin during different agricultural organic wastes compostable environments: It is meaningful carbon sequestration.

Bioresour Technol 2020 Mar 13;299:122596. Epub 2019 Dec 13.

College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The study aims to accelerate the formation of humin (HM) with the addition MnO to achieve carbon sequestration during different material composting. The results indicated that the addition of MnO could improve the concentration of HM by increasing of the content in functional groups during corn straw (CS) and chicken manure (CM) composting. With the addition of MnO, non-aromatic functional groups were responsible for the increase of the HM concentration in CM, while aromatic functional groups were dominating for CS. Although the formation mechanism of HM varied significantly across different materials, the MnO promoted more abundant functional groups to participate the formation of recalcitrant fluorescence components in CS and CM. In addition, the aromatization of HM structure was improved by adding the MnO. Therefore, the addition of MnO not only increase carbon sequestration but also increase the compost product resilience during the decompose of agricultural organic wastes.
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http://dx.doi.org/10.1016/j.biortech.2019.122596DOI Listing
March 2020

Mesoporous g-CN/β-CD nanocomposites modified glassy carbon electrode for electrochemical determination of 2,4,6-trinitrotoluene.

Talanta 2020 Feb 30;208:120410. Epub 2019 Sep 30.

Key Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei, 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei, 230009, PR China.

A novel method using mesoporous g-CN and β-cyclodextrin nanocomposite (mpg-CN/β-CD) for the electrochemical detection of 2,4,6-trinitrotoluene (TNT) is presented. The unique structure of the mesoporous g-CN/β-CD nanocomposite facilitates both mpg-CN electrocatalysis and β-CD inclusion-complexation of the analytes. When compared to GCE or mpg-CN modified GCE, the mpg-CN/β-CD modified glassy carbon electrode exhibits superior performance in the detection of TNT. Hence, we used mpg-CN/β-CD modified GCE for the development of TNT detection method using linear sweep voltammetry at -0.45 V reduction potential with 100 s accumulation time. The TNT calibration curve is linear within the 1-100 μM concentration range and the corresponding sensitivity and LOD values are 0.2 μA/μM and 68 ppb respectively. When p-nitrophenol and o-nitrophenol introduced into the matrix, the mpg-CN/β-CD modified GCE sensor showed high selectivity and sensitivity to the nitroaromatic compounds. Optimized mpg-CN/β-CD modified GCE sensor was used for the detection of TNT in a natural lake water sample with an 96.8% peak recovery. The results shown in work illustrate the potential of using g-CN/β-CD modified GCE sensors in monitoring TNT and other nitroaromatic compounds in environmental analysis.
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http://dx.doi.org/10.1016/j.talanta.2019.120410DOI Listing
February 2020

The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation.

Mol Cell 2020 01 30;77(2):368-383.e7. Epub 2019 Oct 30.

Key Laboratory of Metabolism and Molecular Medicine of Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; The Fifth People's Hospital of Shanghai and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200438, China. Electronic address:

Interphase chromatin is hierarchically organized into higher-order architectures that are essential for gene functions, yet the biomolecules that regulate these 3D architectures remain poorly understood. Here, we show that scaffold attachment factor B (SAFB), a nuclear matrix (NM)-associated protein with RNA-binding functions, modulates chromatin condensation and stabilizes heterochromatin foci in mouse cells. SAFB interacts via its R/G-rich region with heterochromatin-associated repeat transcripts such as major satellite RNAs, which promote the phase separation driven by SAFB. Depletion of SAFB leads to changes in 3D genome organization, including an increase in interchromosomal interactions adjacent to pericentromeric heterochromatin and a decrease in genomic compartmentalization, which could result from the decondensation of pericentromeric heterochromatin. Collectively, we reveal the integrated roles of NM-associated proteins and repeat RNAs in the 3D organization of heterochromatin, which may shed light on the molecular mechanisms of nuclear architecture organization.
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http://dx.doi.org/10.1016/j.molcel.2019.10.001DOI Listing
January 2020

Tunable optical delay with low intensity loss in a cascade structure Er-doped optical fiber amplifier.

Appl Opt 2019 Jul;58(19):5257-5261

In this paper, we propose a new pumping scheme-a dual-frequency laser-pumped cascade structure of Er-doped optical fiber based on the theory of coherent population oscillation. While realizing slow light transmission, the loss of output signal light power is reduced. Using numerical simulation, we compare the effects of a single fiber without pumping, a 980 nm single pumping single fiber, and a dual-frequency laser-pumped cascade structure of Er-doped optical fiber on signal light intensity loss coefficient τ and maximum time delay with the increase of input signal light power. The results show that the signal light intensity loss coefficient τ of the three structures decreases gradually with the increase of input signal light power. However, the transmission of lower signal light intensity loss coefficient τ=0.0823 and a larger time delay of 0.15 ms can be obtained under the dual-frequency laser-pumped cascade structure of Er-doped optical fiber. On the basis of the dual-frequency laser-pumped cascade structure of Er-doped optical fiber, the signal light intensity loss coefficient τ decreases gradually with the increase of pump ratio M (M=P∶P) and the decrease of length ratio N (N=L∶L; L and L represent the length of the first and second cascaded optical fibers, respectively). Moreover, we compare the effects of ion density of the Er-doped optical fiber on the time delay of three structures. The dual-frequency laser-pumped cascade structure of Er-doped optical fiber can provide a larger time delay and smaller signal light intensity loss coefficient τ at low ion density of the Er-doped optical fiber.
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http://dx.doi.org/10.1364/AO.58.005257DOI Listing
July 2019

Reversal of ciprofloxacin-induced testosterone reduction by probiotic microbes in mouse testes.

Gen Comp Endocrinol 2019 12 3;284:113268. Epub 2019 Sep 3.

Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang 050017, China.

CPFX is a highly effective antibiotic, but it has been reported to significantly impair both testicular function and structure in rats. In this study, we assessed reversal of CPFX-induced variation in mice testicular structure and testosterone synthesis by probiotic microbes in the infected model and normal model. We detected testicular weight, testicular structure and Leydig cell variables in numbers. We detected the levels of serum testosterone and steroidogenic enzymes, as well as DBC1, Sirt1, NF-κB, and related redox state and inflammatory response in the testes. The results showed that probiotic microbes had significantly elevated serum testosterone levels and steroidogenic enzymes, higher Sirt1, anti-oxidative enzymes and anti-inflammatory cytokine expression, and lower NF-κB, DBC1, oxidative damage, pro-inflammatory cytokine expression. The results suggest that the testis-protective, antiinflammatory and antioxidation effects of probiotics largely resulted from its ability to decrease oxidative stress and preserve antioxidant activity by stabilizing antioxidant defense systems, reducing oxidative damage and inflammatory response.
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http://dx.doi.org/10.1016/j.ygcen.2019.113268DOI Listing
December 2019

The roles of p38 MAPK → COX2 and NF-κB → COX2 signal pathways in age-related testosterone reduction.

Sci Rep 2019 07 22;9(1):10556. Epub 2019 Jul 22.

Department of cell biology, Hebei Medical University, Shijiazhuang, 050017, China.

In our study, we explored changes in the redox status and inflammatory response in the testes of the SAMP8 model of varying ages (2, 4, 8, 10 months old) compared with control mice SAMR1 by the methods of immunohistochemical staining, Western blotting, RT-PCR and Luminex multi-analyte cytokine profiling. We found that as ROS and inflammation levels increased during aging, steroidogenic enzymes (StAR and P450scc) reduced and led to the decline of testosterone production eventually. The pathways of P38 MAPK → COX2 and NF-κB → COX2 were detected by using specific inhibitors of SB203580 and Bay 11-7082 in isolated Leydig cells. These results indicated that activation of both p38 MAPK → COX2 and NF-κB → COX2 signaling pathways are functionally linked to the oxidative stress response and chronic inflammation during aging, and mediate their inhibitory effects on testosterone production.
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http://dx.doi.org/10.1038/s41598-019-46794-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646396PMC
July 2019

Disruption of nuclear speckles reduces chromatin interactions in active compartments.

Epigenetics Chromatin 2019 07 17;12(1):43. Epub 2019 Jul 17.

The Fifth People's Hospital of Shanghai, and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.

Background: Nuclei of eukaryotes contain various higher-order chromatin architectures and nuclear bodies (NBs), which are critical for proper nuclear functions. Recent studies showed that active chromatin regions are associated with nuclear speckles (NSs), a type of NBs involved in RNA processing. However, the functional roles of NSs in 3D genome organization remain unclear.

Results: Using mouse hepatocytes as the model, we knocked down SRRM2, a core protein component scaffolding NSs, and performed Hi-C experiments to examine genome-wide chromatin interactions. We found that Srrm2 depletion disrupted the NSs and changed the expression of 1282 genes. The intra-chromosomal interactions were decreased in type A (active) compartments and increased in type B (repressive) compartments. Furthermore, upon Srrm2 knockdown, the insulation of TADs was decreased specifically in active compartments, and the most significant reduction occurred in A1 sub-compartments. Interestingly, the change of intra-TAD chromatin interactions upon Srrm2 depletion was not associated with the alteration of gene expression.

Conclusions: We show that disruption of NSs by Srrm2 knockdown causes a global decrease in chromatin interactions in active compartments, indicating critical functions of NSs in the organization of the 3D genome.
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http://dx.doi.org/10.1186/s13072-019-0289-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636040PMC
July 2019

Research on Adaptive Multi-Source Information Fault-Tolerant Navigation Method Based on No-Reference System Diagnosis.

Sensors (Basel) 2019 Jul 1;19(13). Epub 2019 Jul 1.

College of Automation Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China.

In order to obtain accurate and optimized navigation sensor information, it is necessary to study information fusion and fault diagnosis with high reliability, high precision and high autonomy, and then to propose a rapid and accurate intelligent decision-making scheme based on multi-source and heterogeneous navigation information. In view of the existing fault-tolerant navigation federated filter structure, the method of assuming the reference system (inertial navigation system) to be fault-free and then diagnosing the measuring sensor fault is generally adopted. Considering that the structure of the filter can't detect and isolate the faults of the inertial navigation system, the performance of the MEMS inertial navigation system declines due to complex environments resulting from vibrations and temperature changes; additionally, external interference may lead to the direct failure of the MEMS inertial device. Therefore, this paper studies a fault-tolerant navigation method based on a no-reference system. For the sensor sub-system of a custom micro air vehicle (MAV), a fault detection method based on a reference-free system is proposed. Based on the fault type analysis, some improvements have been made to the existing residual chi-square detection method, and an interactive residual fault detection method with distributed states is proposed. On this basis, aiming at the characteristics of a reference-free system, the weight distribution scheme of the reference system and the tested systems are studied, and a self-regulation filter fusion and fault detection method based on reference-free system is designed.
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http://dx.doi.org/10.3390/s19132911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651474PMC
July 2019