Dr Phetole Walter Mahasha, PhD in Medical Immunology - South African Medical Research Council (SAMRC) - Senior Scientist

Dr Phetole Walter Mahasha

PhD in Medical Immunology

South African Medical Research Council (SAMRC)

Senior Scientist

Cape Town, Western Province | South Africa

Main Specialties: Biochemical Genetics, Biology, Clinical & Laboratory Immunology, Epidemiology, Infectious Disease, Medical Genetics, Medical Microbiology, Public Health

Additional Specialties: Medical Immunology, Medical Biochemistry, Biochemistry and Microbiology

ORCID logohttps://orcid.org/0000-0002-5750-3595

Dr Phetole Walter Mahasha, PhD in Medical Immunology - South African Medical Research Council (SAMRC) - Senior Scientist

Dr Phetole Walter Mahasha

PhD in Medical Immunology
Introduction

I am currently employed by the South African Medical Research Council (SAMRC), Cochrane South Africa Centre as a Senior Scientist where I prepare research findings for publication in leading academic journals in collaboration with other Unit staff and lead/coordinate ongoing projects on vaccine acceptance and uptake. I am a registered Professional Natural Scientist (registration number: 119189) holding a PhD in Medical Immunology and an M.Sc. degree in Medical Sciences (Medical Biochemistry). I have also completed training courses in ICH GCP, CITI, Good Clinical Practices and Protecting Human Research Participants, and Good Clinical Laboratory Practice (GCLP). I have over 8 years’ research experience on HIV/AIDS and over two years on TB. Dr. I was previously employed by EQUIP Innovation for Health (Right to Care) as a Senior Research Manager working in 17 countries across Africa, South East Asia, Eastern Europe and the Caribbean towards achieving the UNAIDS 90- 90-90 targets, the project is funded by USAID which include HIV Self-testing (HIV)-Co-PI, Multi-month Scripting and Dispensing (MMSD) and other projects include Pre-exposure prophylaxis (PreP) demo projects (Namibia), Hepatitis C Virus projects (HCV) (Ukraine and Myanmar) and Costing and cost-effectiveness assessments of various innovative interventions. I also was previously employed by the University of Limpopo as a Senior Lecturer in the Department of Pre-Clinical Sciences (Medical Biochemistry), lectured Medical and Nutritional Biochemistry.

I have completed an NIH FIC Postdoctoral Research Fellowship with the University of Venda (South Africa), HIV/AIDS & Global Health Research Programme, Department of Microbiology and the University fo Virginia (USA), Center for Global Health where I worked as a Genetic Epidemiology/Statistical Genetics and Metabonomics Research Fellow conducting analytical and genetic epidemiological research with existing data sets. Primary analytical methods included statistical modelling of common genetic variant factors influencing a trait or clinical outcome and statistical modelling of rare genetic variant factors influencing a trait or clinical outcome including the use of burden or aggregate marker test. I worked under the mentorship of Drs. Richard Guerrant, Infectious Diseases and International Health and Josyf Mychaleckyj, Public Health Sciences Administration at UVA and Dr. Bessong at Univen collaboratively analyzing existing GWAS samples from Gambia and Sierra Leone with local South African children after obtaining and isolating salivary DNA and another Postdoctoral fellowship with the National Institute for Communicable Diseases, in the Cell Biology Laboratory within the Centre for HIV and STIs.

I graduated with a PhD in Medical Immunology with the University of Pretoria (South Africa) at a graduation ceremony held on the 5th of September 2014, with a research project titled: “Compartmentalization, Adaptive Evolution and Therapeutic Response of HIV-1 in the Gastrointestinal Tract (GIT) of African patients infected with Subtype C: Implications for the Enhancement of Therapeutic Efficacy”. I completed a B.Sc. degree with the University of the North (now University of Limpopo), where I majored in Microbiology and Biochemistry. During my Honours work at the same institution, I completed a project on cancer research titled, “Determination of the influence of lithium chloride (LiCl) on pro- and anti-apoptotic proteins in PK cells (15)”. These studies solidified my desire to pursue a career in health research, and highlighted the need to focus on transitional research that is of critical importance to South Africa and the developing world. As a result of a growing interest in HIV-1/AIDS and AIDS-related infections including tuberculosis and malnutrition and enteric diseases, I seized the opportunity to continue my scientific training in TB research with Dr. Gey van Pittius and Professor Rob Warren at the University of Stellenbosch (South Africa), where I successfully co-designed and completed a research project entitled, “Trafficking of the Mycobacterium tuberculosis PE and PPE proteins”. I graduated for my M.Sc. in Medical Sciences at a graduation ceremony held at the University of Stellenbosch in December 2007.

These research projects provided me with a range of different skills that are necessary for the development of a successful career in health research, including laboratory-based experience in tissue and cell culture, DNA isolation, immunohistochemistry, SDS-PAGE, Western blotting, PCR, site-directed mutagenesis, cloning, and gene knockout techniques, the use of statistical genomics/genetic epidemiology techniques and tools. Teaching experience has ranged from assisting undergraduate students with their practicals (as a lab demonstrator) to the marking of their scripts and the supervision of undergraduate research activities. I also facilitated lectures on HIV and TB pathology and pathogenesis and on how to write a scientific abstract, paper/manuscript and dissertation/thesis. I also learned a lot working in the field, managing a project for collecting saliva samples to determine the impact of malnutrition and enteric diseases on child growth and development.

Through out my studies and research career over the years financial support was provided by the NIH Forgaty International Center, South African National Research Foundation, South African Medical Research Council, Poliomyelitis Research Foundation, Department of Immunology University of Pretoria, University of Pretoria Postgraduate Scholarship, University of Stellenbosch Postgraduate Bursary, DST/NRF Centre of Excellence in Biomedical Tuberculosis Research and US/MRC Centre for Molecular and Cellular Biology Bursary. I have published more than 8 articles/papers published in reputable journals and have been serving as an editorial board member of repute in international journals.

My Research Interest is inVaccinology, Infectious Diseases, HIV/AIDS Research, Tuberculosis (TB) Research, Malnutrion and Enteric Pathogens Research, Clinical Research and Clinical Trials and Public Health research.

Primary Affiliation: South African Medical Research Council (SAMRC) - Cape Town, Western Province , South Africa

Specialties:

Additional Specialties:

Research Interests:


View Dr Phetole Walter Mahasha’s Resume / CV
Metrics

6

Publications

797

Profile Views

122

Reads

168

PubMed Central Citations

Education
Sep 2014
University of Pretoria
PhD
Dec 2007
University of Stellenbosch
M.Sc.
Oct 2004
University of Limpopo
B.Sc. Honours
Apr 2003
University of Limpopo
B.Sc.
Top co-authors
Chris Seebregts
Chris Seebregts

Jembi Health Systems NPC

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Guido Poli
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San Raffaele Scientific Institute

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Sharon Cassol
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University of KwaZulu-Natal

2
Theresa Rossouw
Theresa Rossouw

University of KwaZulu-Natal

2
Edana Cassol
Edana Cassol

San Raffaele Scientific Institute

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Susan Malfeld
Susan Malfeld

University of Pretoria

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Massimo Alfano
Massimo Alfano

San Raffaele Scientific Institute

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Abdallah M Abdallah
Abdallah M Abdallah

Pathogen Genomics Laboratory

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Theo Verboom
Theo Verboom

Vrije Universiteit Medical Center

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Publications

6Publications

122Reads

168PubMed Central Citations

Access to HIV care and treatment for migrants between Lesotho and South Africa: a mixed methods study.

BMC Public Health 2018 May 29;18(1):668. Epub 2018 May 29.

EQUIP - Innovation for Health, 1006 Lenchen Avenue North, Centurion, South Africa.

View Article
May 2018
23 Reads
2.26 Impact Factor

HIV drug resistance levels in adults failing first-line antiretroviral therapy in an urban and a rural setting in South Africa.

HIV Med 2017 02 28;18(2):104-114. Epub 2016 Jun 28.

Africa Centre Population Health, University of KwaZulu-Natal, South Africa.

View Article
February 2017
34 Reads
2 PubMed Central Citations(source)
3.99 Impact Factor

Impaired CD4+ T-cell restoration in the small versus large intestine of HIV-1-positive South Africans receiving combination antiretroviral therapy.

J Infect Dis 2013 Oct 6;208(7):1113-22. Epub 2013 Jun 6.

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston MA 02215, USA.

View Article
October 2013
15 Reads
7 PubMed Central Citations(source)
6.00 Impact Factor

Persistent microbial translocation and immune activation in HIV-1-infected South Africans receiving combination antiretroviral therapy.

J Infect Dis 2010 Sep;202(5):723-33

MRC Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria and the Tshwane Academic Division of the National Health Laboratory Service, Pretoria, South Africa.

View Article
September 2010
2 Reads
79 PubMed Central Citations(source)
6.00 Impact Factor

PPE and PE_PGRS proteins of Mycobacterium marinum are transported via the type VII secretion system ESX-5.

Mol Microbiol 2009 Aug 7;73(3):329-40. Epub 2009 Jul 7.

Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands.

View Article
August 2009
48 Reads
80 PubMed Central Citations(source)
4.42 Impact Factor

10 Reads
Top co-authors
Chris Seebregts
Chris Seebregts

Jembi Health Systems NPC

2
Guido Poli
Guido Poli

San Raffaele Scientific Institute

2
Sharon Cassol
Sharon Cassol

University of KwaZulu-Natal

2
Theresa Rossouw
Theresa Rossouw

University of KwaZulu-Natal

2
Edana Cassol
Edana Cassol

San Raffaele Scientific Institute

2
Susan Malfeld
Susan Malfeld

University of Pretoria

2
Massimo Alfano
Massimo Alfano

San Raffaele Scientific Institute

1
Abdallah M Abdallah
Abdallah M Abdallah

Pathogen Genomics Laboratory

1
Theo Verboom
Theo Verboom

Vrije Universiteit Medical Center

1