Publications by authors named "Petro Yu Barzilovich"

2 Publications

  • Page 1 of 1

Polyamines Counteract Carbonate-Driven Proteasome Stalling in Alkaline Conditions.

Biomolecules 2020 11 24;10(12). Epub 2020 Nov 24.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

Cancer cells tend to increase intracellular pH and, at the same time, are known to intensively produce and uptake polyamines such as spermine. Here, we show that various amines, including biogenic polyamines, boost the activity of proteasomes in a dose-dependent manner. Proteasome activity in the classical amine-containing buffers, such as 2-(N-morpholino)ethanesulfonic acid (MES), Tris, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glycylglycine, bis-Tris propane, and bicine, has a skewed distribution with a maximum at pH of 7.0-8.0. The activity of proteasomes in buffers containing imidazole and bis-Tris is maintained almost on the same level, in the pH range of 6.5-8.5. The third type of activation is observed in buffers based on the amino acids arginine and ornithine, as well as the natural polyamines spermine and spermidine. Proteasome activity in these buffers is dramatically increased at pH values greater than 7.5. Anionic buffers such as phosphate or carbonate, in contrast, inhibit proteasome activity during alkalization. Importantly, supplementation of a carbonate-phosphate buffer with spermine counteracts carbonate-driven proteasome stalling in alkaline conditions, predicting an additional physiological role of polyamines in maintaining the metabolism and survival of cancer cells.
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http://dx.doi.org/10.3390/biom10121597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760842PMC
November 2020

Cation-π and lone pair-π interactions combined in one: the first experimental evidence of (H3O-lp)+···π-system binding in a crystal.

Chemphyschem 2011 Nov 14;12(16):2895-8. Epub 2011 Jun 14.

A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119991, Vavilova Str., 28, Moscow, Russia.

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http://dx.doi.org/10.1002/cphc.201100294DOI Listing
November 2011
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