Dr. Perpetua Pinto-do-Ó, PhD - i3S - Instituto de Investigação e Inovação em Saúde & INEB - Instituto Nacional de Engenharia Biomédica Universidade do Porto - Research Scientist

Dr. Perpetua Pinto-do-Ó

PhD

i3S - Instituto de Investigação e Inovação em Saúde & INEB - Instituto Nacional de Engenharia Biomédica Universidade do Porto

Research Scientist

Porto, Porto | Portugal

Main Specialties: Biochemical Genetics, Biology, Cardiovascular Disease, Hematology

Additional Specialties: Stem Cell Biology, Cellular Biology

ORCID logohttps://orcid.org/0000-0002-6477-6030


Top Author

Dr. Perpetua Pinto-do-Ó, PhD - i3S - Instituto de Investigação e Inovação em Saúde & INEB - Instituto Nacional de Engenharia Biomédica Universidade do Porto - Research Scientist

Dr. Perpetua Pinto-do-Ó

PhD

Introduction

Perpetua Pinto-do-Ó is an Investigator at the INEB (Instituto de Engenharia Biomédica) & I3S (Instituto de Investigação e Inovação em Saúde, Universidade do Porto), where she leads a research group focused on Stem Cell and Regenerative Biology. Perpetua differentiated background enables her to explore both basic and applied science from a multitude of perspectives. Shortly, after completing a degree in Biology and a three years internship in Pharmacology (Faculty of Medicine, Porto University/Department for Molecular Medicine, Karolinska Institute, Stockholm), Perpetua enrolled in the Gulbenkian PhD Program in Biology and Medicine (IGC, Oeiras, Portugal) in 1995. This was her opportunity to get in touch with Developmental Biology and Stem Cells, an area that she pursed, and in which got a PhD degree from Umeå University, Sweden (1996-2002). During this period, Perpetua was involved in the development of two cell lines, one derived from embryonic stem (ES) cells (A.K.A. HPC-7) and the other from adult bone marrow stem/progenitor cells (A.K.A. HPC-5), which proved as an in vitro immortalized source of a high-fidelity proxy for mouse and human hematopoietic stem/progenitor cells. These lines were henceforth used by several laboratories worldwide remaining up-today as a reference in the field accounting so far over 140 citations. Afterwards, she undertook a postdoctoral training at Instituto de Biologia Molecular e Celular-IBMC (2003-2006), which allowed her to develop further skills in molecular immunology and infection. By the end of 2007, she became a researcher at INEB, where she launched a Stem Cell and Regenerative Biology research line.
Along with her long-time interest on basic stem cell biology mechanisms, Perpetua got interested on understanding the role stem/progenitor cells allocated to specific tissue/organs might have on the regeneration in the adult organism. Therefore, and as a counterpart to her experience with the highly regenerative hematopoietic system, her team at INEB initially focused on the heart (a poorly regenerative/repair system) as a model in this investigation. As a result, a series of in vivo and in vitro tools were explored, implemented and newly-created thereby allowing the establishment of a new research program on Cardiovascular Biology and Repair at the Institute. Currently, and following the recent resuming of the investigation on hematopoiesis, her lab is equipped with a highly regenerative and a poorly regenerative/repair working models. Her longstanding scientific interests are comparative studies focused on “the whether and how” stem/progenitor cells keep the homeostasis and functionally restore distinct organ-systems.
Besides her activities as a researcher, Perpetua has been the President of the Portuguese Society for Stem Cells and Cell Therapy (SPCE-TC) (http://spce-tc.org/) for the biennium 2016-2017, re-elected for the 2018-2019 biennium. Moreover, since 2008 she has been responsible/collaborator for teaching distinct courses on cell engineering and stem cells in regenerative therapies at the University of Porto; she is an Adjunct Professor (2012-) and Invited Professor (2018-) at Instituto de Ciências Biomédicas Abel Salazar (ICBAS) of the University of Porto (2012-), where she is the coordinator of the "Stem Cell Biology" course.

Primary Affiliation: i3S - Instituto de Investigação e Inovação em Saúde & INEB - Instituto Nacional de Engenharia Biomédica Universidade do Porto - Porto, Porto , Portugal

Specialties:

Additional Specialties:

Research Interests:


View Dr. Perpetua Pinto-do-Ó’s Resume / CV

Education

Jan 2003 - Jul 2006
Institute for Molecular and Cell Biology
Postdoctoral fellow (FCT)
Infection and Immunity
Oct 1996 - Nov 2002
Umea Universitet
PhD student
Stem Cell Biology
Oct 1995 - Sep 1996
Instituto Gulbenkian de Ciencia
Post-graduate Diploma (1st year PhD Gulbenkian Programme in Biology and Medicine)
Graduate Studies, IGC, Oeiras, Portugal
Sep 1994 - Sep 1995
Karolinska Institutet
Guest researcher (Swedish Institute fellwoship)
Molecular Medicine (Pharmacology-Biochemistry)
Jan 1993 - Sep 1994
Faculdade de Medicina da Universidade do Porto
Internship (Pharmacology)
Instituto de Farmacologia e Terapêutica
Dec 1986 - Dec 1992
Universidade do Porto Faculdade de Ciências
Degree in Biology
Scientific branch

Experience

Apr 2019
Principal Investigator
Group leader
Stem Cell in Regenerative Biology and Repair (SCRIpt) group, i3S - Instituto de Investigação e Inovação
Dec 2007 - Jan 2018
Assistant Researcher (with tenure)
Group leader
Stem Cell in Regenerative Biology and Repair (SCRIpt) group, i3S - Instituto de Investigação e Inovação
Jan 2018
Invited Assistant Professor
Stem Cell Biology course, coordinator
Instituto de Ciências Biomédicas Abel Salazar [ICBAS], University of Porto
Oct 2014
Invited Scientist

Institut Pasteur, Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot
Dec 2012
Assistant Researcher (with tenure)
Team leader
Former INEB "NewTherapies" group (2009-2014); team leader and core CV, i3S "Microenvironments for New Therapies" group (2014-2018)
Jan 2012 - Apr 2012
Adjunct Professor
Adjunct Professor
Instituto de Ciências Biomédicas Abel Salazar [ICBAS], University of Porto
Dec 2007
Assistant Researcher (Ciência2007 awardee)
Stem cell biology coordinator/team
Instituto Nacional de Engenharia Biomédica, Universidade do Porto
Jan 2003
Postdoctoral fellow (Immunology of Infection)

Institute for Molecular and Cell Biology (IBMC)
Nov 2002
Ph.D. degree
Ph.D. student
Umeå University, Department of Molecular Biology (CMB), Sweden (1996-2002)

Publications

38Publications

736Reads

21Profile Views

160PubMed Central Citations

Establishing a Link between Endothelial Cell Metabolism and Vascular Behaviour in a Type 1 Diabetes Mouse Model.

Cell Physiol Biochem 2019 ;52(3):503-516

Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine of the University of Porto, Porto, Portugal.

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http://dx.doi.org/10.33594/000000036DOI Listing
April 2019
9 Reads
2.875 Impact Factor

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies.

J Vis Exp 2019 03 21(145). Epub 2019 Mar 21.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto; INEB - Instituto de Engenharia Biomédica, Universidade do Porto; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto;

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http://dx.doi.org/10.3791/56924DOI Listing
March 2019
5 Reads

Neonatal Apex Resection Triggers Cardiomyocyte Proliferation, Neovascularization and Functional Recovery Despite Local Fibrosis.

Stem Cell Reports 2018 03 1;10(3):860-874. Epub 2018 Mar 1.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal; INEB - Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S22136711183006
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http://dx.doi.org/10.1016/j.stemcr.2018.01.042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918841PMC
March 2018
14 Reads

Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate.

Stem Cell Reports 2017 07 22;9(1):136-148. Epub 2017 Jun 22.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal. Electronic address:

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http://dx.doi.org/10.1016/j.stemcr.2017.05.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511108PMC
July 2017
87 Reads

Restoring heart function and electrical integrity: closing the circuit.

NPJ Regen Med 2017 7;2. Epub 2017 Apr 7.

i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

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http://dx.doi.org/10.1038/s41536-017-0015-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665620PMC
April 2017
4 Reads

Decellularized human colorectal cancer matrices polarize macrophages towards an anti-inflammatory phenotype promoting cancer cell invasion via CCL18.

Biomaterials 2017 04 5;124:211-224. Epub 2017 Feb 5.

i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, 4200-135, Portugal; INEB-Institute of Biomedical Engineering, University of Porto, Porto, 4200-135, Portugal; Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, 4200-319, Portugal. Electronic address:

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http://dx.doi.org/10.1016/j.biomaterials.2017.02.004DOI Listing
April 2017
5 Reads
8.557 Impact Factor

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult.

Biomaterials 2016 10 30;104:52-64. Epub 2016 Jun 30.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4200-135, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto 4200-135, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Porto 4050-313, Portugal; Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur. Institut Pasteur, Paris, France. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S01429612163032
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http://dx.doi.org/10.1016/j.biomaterials.2016.06.062DOI Listing
October 2016
6 Reads
8.557 Impact Factor

Optimized Heart Sampling and Systematic Evaluation of Cardiac Therapies in Mouse Models of Ischemic Injury: Assessment of Cardiac Remodeling and Semi-Automated Quantification of Myocardial Infarct Size.

Curr Protoc Mouse Biol 2015 Dec 2;5(4):359-391. Epub 2015 Dec 2.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

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http://dx.doi.org/10.1002/9780470942390.mo140293DOI Listing
December 2015
10 Reads
1 Citation

Building and repairing the heart: what can we learn from embryonic development?

Biomed Res Int 2014 17;2014:679168. Epub 2014 Apr 17.

Instituto de Engenharia Biomédica (INEB), Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal ; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.

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http://dx.doi.org/10.1155/2014/679168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016833PMC
January 2015
11 Reads
2 Citations

Sca-1+ cardiac progenitor cells and heart-making: a critical synopsis.

Stem Cells Dev 2014 Oct 14;23(19):2263-73. Epub 2014 Jul 14.

1 Stem-Cell Microenvironments in Repair/Regeneration Team, Microenvironments for NewTherapies Group, INEB-Instituto Nacional de Engenharia Biomédica, Universidade do Porto , Porto, Portugal .

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http://dx.doi.org/10.1089/scd.2014.0197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172562PMC
October 2014
13 Reads
11 Citations
3.730 Impact Factor

Stable phenotype and function of immortalized Lin-Sca-1+ cardiac progenitor cells in long-term culture: a step closer to standardization.

Stem Cells Dev 2014 May 14;23(9):1012-26. Epub 2014 Feb 14.

1 INEB-Instituto de Engenharia Biomédica, Universidade do Porto , Porto, Portugal .

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http://dx.doi.org/10.1089/scd.2013.0305DOI Listing
May 2014
36 Reads
5 Citations
3.730 Impact Factor

Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure.

ACS Nano 2014 Mar 7;8(3):2033-47. Epub 2014 Feb 7.

Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan.

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http://dx.doi.org/10.1021/nn4058984DOI Listing
March 2014
35 Reads
21 Citations
12.881 Impact Factor

Primary bone marrow mesenchymal stromal cells rescue the axonal phenotype of Twitcher mice.

Cell Transplant 2014 Feb 27;23(2):239-52. Epub 2013 Jun 27.

Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal.

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http://dx.doi.org/10.3727/096368913X669752DOI Listing
February 2014
32 Reads
5 Citations
3.570 Impact Factor

Adult stem cells and biocompatible scaffolds as smart drug delivery tools for cardiac tissue repair.

Curr Med Chem 2013 ;20(28):3429-47

Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan.

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http://dx.doi.org/10.2174/09298673113209990032DOI Listing
February 2014
9 Reads
2 Citations
3.853 Impact Factor

MIQuant--semi-automation of infarct size assessment in models of cardiac ischemic injury.

PLoS One 2011 30;6(9):e25045. Epub 2011 Sep 30.

Instituto de Engenharia Biomédica (INEB), Universidade do Porto, Porto, Portugal.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025045PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184116PMC
March 2012
8 Reads
5 Citations
3.234 Impact Factor

Systemic delivery of bone marrow-derived mesenchymal stromal cells diminishes neuropathology in a mouse model of Krabbe's disease.

Stem Cells 2011 Nov;29(11):1738-51

Nerve Regeneration Group, IBMC-Instituto de Biologia Molecular e Celular,Universidade do Porto, Porto, Portugal.

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http://dx.doi.org/10.1002/stem.724DOI Listing
November 2011
36 Reads
11 Citations
6.523 Impact Factor

TNF-alpha regulates the effects of irradiation in the mouse bone marrow microenvironment.

PLoS One 2010 Feb 1;5(2):e8980. Epub 2010 Feb 1.

Angiogenesis Laboratory, Centro de Investigação em Patobiologia Molecular, Instituto Portugues de Oncologia de Francisco Gentil, Lisboa, Portugal.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008980PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813873PMC
February 2010
18 Reads
12 Citations
3.234 Impact Factor

The thymus as a target for mycobacterial infections.

Microbes Infect 2007 Nov-Dec;9(14-15):1521-9. Epub 2007 Aug 31.

Laboratory of Microbiology and Immunology of Infection, Institute for Molecular and Cell Biology (IBMC), University of Porto, Rua do Campo Alegre 823, 4150-084 Porto, Portugal.

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http://dx.doi.org/10.1016/j.micinf.2007.08.006DOI Listing
March 2008
9 Reads
17 Citations
2.861 Impact Factor

Establishment of multipotent hematopoietic progenitor cell lines from ES cells differentiated in vitro.

Methods Enzymol 2003 ;365:202-14

Umeå Centre for Molecular Medicine, Umeå University, 901 87 Umeå, Sweden.

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http://dx.doi.org/10.1016/s0076-6879(03)65014-7DOI Listing
January 2004
8 Reads
2 Citations
2.090 Impact Factor

Lhx2 expression in hematopoietic progenitor/stem cells in vivo causes a chronic myeloproliferative disorder and altered globin expression.

Haematologica 2003 Dec;88(12):1336-47

Umeå Center for Molecular Medicine, Umeå University, 901 87 Umeå, Sweden.

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December 2003
8 Reads
5 Citations
5.870 Impact Factor

Hematopoietic progenitor/stem cells immortalized by Lhx2 generate functional hematopoietic cells in vivo.

Blood 2002 Jun;99(11):3939-46

Department of Molecular Biology, Umeå University, Sweden.

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http://dx.doi.org/10.1182/blood.v99.11.3939DOI Listing
June 2002
7 Reads
19 Citations
10.452 Impact Factor

Multipotent hematopoietic progenitor cells immortalized by Lhx2 self-renew by a cell nonautonomous mechanism.

Exp Hematol 2001 Aug;29(8):1019-28

Department of Microbiology, University of Umeå, Umeå, Sweden.

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http://dx.doi.org/10.1016/s0301-472x(01)00666-xDOI Listing
August 2001
4 Reads
2.475 Impact Factor

Expression of the LIM-homeobox gene LH2 generates immortalized steel factor-dependent multipotent hematopoietic precursors.

EMBO J 1998 Oct;17(19):5744-56

Department of Microbiology, University of Umeå, 901 87 Umeå, Sweden.

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http://dx.doi.org/10.1093/emboj/17.19.5744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170902PMC
October 1998
3 Reads
10.434 Impact Factor

Short-term vs. sustained inhibition of proximal tubule Na,K-ATPase activity by dopamine: cellular mechanisms.

Clin Exp Hypertens 1997 Jan-Feb;19(1-2):73-86

Department of Molecular Medicine, Karolinska Institute, Stockholm, Sweden.

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http://dx.doi.org/10.3109/10641969709080805DOI Listing
April 1997
3 Reads
1.456 Impact Factor

Studies on the pharmacology of the inward transport of L-DOPA in rat renal tubules.

Br J Pharmacol 1996 Jun;118(3):741-7

Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1909740PMC
http://dx.doi.org/10.1111/j.1476-5381.1996.tb15462.xDOI Listing
June 1996
2 Reads
4.842 Impact Factor

Antagonistic actions of renal dopamine and 5-hydroxytryptamine: increase in Na+, K(+)-ATPase activity in renal proximal tubules via activation of 5-HT1A receptors.

Br J Pharmacol 1996 Mar;117(6):1199-203

Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1909776PMC
http://dx.doi.org/10.1111/j.1476-5381.1996.tb16716.xDOI Listing
March 1996
1 Read
4.842 Impact Factor

Antagonistic actions of renal dopamine and 5-hydroxytryptamine: effects of amine precursors on the cell inward transfer and decarboxylation.

Br J Pharmacol 1996 Mar;117(6):1187-92

Department of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1909791PMC
http://dx.doi.org/10.1111/j.1476-5381.1996.tb16714.xDOI Listing
March 1996
4 Reads
4.842 Impact Factor

Assessment of renal dopaminergic system activity during cyclosporine A administration in the rat.

Br J Pharmacol 1995 Aug;115(8):1349-58

Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1908888PMC
http://dx.doi.org/10.1111/j.1476-5381.1995.tb16623.xDOI Listing
August 1995
2 Reads
4.842 Impact Factor

Birth dates.

Nature 1995 Aug;376(6539):381

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http://dx.doi.org/10.1038/376381g0DOI Listing
August 1995
3 Reads
42.351 Impact Factor

Studies on the nature of the antagonistic actions of dopamine and 5-hydroxytryptamine in renal tissues.

Hypertens Res 1995 Jun;18 Suppl 1:S47-51

Institute of Pharmacology & Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://dx.doi.org/10.1291/hypres.18.supplementi_s47DOI Listing
June 1995
3 Reads
2.658 Impact Factor

Cell inward transport of L-DOPA and 3-O-methyl-L-DOPA in rat renal tubules.

Br J Pharmacol 1994 Jun;112(2):611-5

Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1910353PMC
http://dx.doi.org/10.1111/j.1476-5381.1994.tb13118.xDOI Listing
June 1994
4 Reads
4.842 Impact Factor